The invention discloses a glass plate sampler for collecting micro-surface water samples, and the sampler comprises a glass plate and a fixed sampling bracket, wherein the fixed sampling bracket comprises an outer frame bracket, a first glass scraper, a first plastic plate, a middle partition, a water sample collecting bottle, a fixed partition, a second glass scraper and a second plastic plate; the first plastic plate and the second plastic plate are respectively disposed on both sides of the outer frame bracket, the first glass scraper is disposed on the vertical edge of the first plastic plate, the second glass scraper is disposed on the vertical edge of the second plastic plate, a gap is formed between the two glass scrapers; the middle partition is provided with a circular hole, whichis aligned with the first glass scraper and the second glass scraper; the fixed partition is disposed at the lower part of the outer frame bracket, the bottom of the water sample collecting bottle isplaced on the fixed partition, and the bottle mouth of the water sample collecting bottle is aligned with the circular hole. The device has the advantages of simple structure, low cost, small volume,light weight, convenient carrying and single operation, and is suitable for the research of micro-surface environmental and ecological aspects such as oceans, lakes and estuaries.