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This invention provides a multi-to-multi drug screening method for CRISPRa / i GPCR libraries based on a three-dimensional spatial hybrid screening model and its applications, belonging to the field of biomedical technology. The method includes the following steps: Step (1): Constructing a screening cell pool; Step (2): Classifying the drugs to be screened; Step (3): Constructing a drug hybrid screening model; Step (4): Grouping drugs according to mutually perpendicular X, Y, and Z planes, with drugs in each plane mixed to form independent pools; Step (5): Processing each pool into a cell pool; Step (6): Detecting and analyzing the changes in sgRNA abundance in each pool; Step (7): Decoding to determine the drug-GPCR interaction pairs. This invention offers higher screening throughput, realizes a multi-target, multi-drug screening mode, reduces costs and time, and enables large-scale research on drug-gene interaction networks, which is of great significance for drug research and development.

The present application relates to the technical field of bridge engineering test, in particular to a model test device and test method for force state of a rotating cable at a rotating cable saddle, the model test device comprising a force applying mechanism, a horizontal turning device, a rotating cable saddle device and an anchoring device, the force applying mechanism being used to connect with one end of a main cable test piece and apply tension to the main cable test piece, the anchoring device being used to fix the other end of the main cable test piece, the horizontal turning device and the rotating cable saddle device being arranged between the force applying mechanism and the anchoring device and being used for winding of the main cable test piece, the horizontal turning device being used to turn the main cable test piece from vertical to horizontal and enter the rotating cable saddle device horizontally and tangentially, and the rotating cable saddle device being used to simulate a rotating cable saddle so that the main cable test piece tangentially leaves the rotating cable saddle device. The present application realizes simulation test of different force states of the main cable of a single-tower single-cable suspension bridge at the rotating cable saddle.