14results about How to "Realize research" patented technology

The invention discloses a Neper-DEM-based apparent crystal rock modeling and calculation method with a bedding structure, relates to the field of numerical calculation of a tunnel engineering technology, and solves the problem that a simulation and calculation method for apparent crystal rocks with transverse isotropy such as gneiss and granite gneiss is lacked in the prior art. The method comprises the following steps: constructing a polycrystal structure with crystal characteristics in Neper, and generating a geometric parameter text of crystal grains from the polycrystal structure; constructing a discrete element rock sample model in the DEM; performing a numerical simulation test on the discrete element rock sample model to obtain a stress-strain curve and a sample failure mode, wherein the numerical simulation is realized by adopting a Brazilian splitting test and a triaxial test; according to a numerical simulation result, discrete element sample calculation parameters are calibrated, and the effectiveness of the modeling and calculation method of the discrete element rock sample model is verified; according to the modeling method, construction and assignment of the crystal structure and the bedding structure are achieved at the same time, so that the rock structure is closer to a real rock structure.

The invention relates to the technical field of biology, in particular to an agrobacterium tumefaciens-mediated open cotton hairy root transformation method which comprises the following steps: selecting eight-day-old cotton seedlings, transversely cutting a hypocotyledonary axis 0.5-1cm above a rhizome junction, and cutting off an underground part; then, agrobacterium rhizogenes liquid is injected into the notch of the hypocotyl, the notch of the hypocotyl is subjected to vacuum filtration treatment, then seedlings are cut into wet vermiculite and placed in a seedling raising tray, a proper amount of clear water is added into the seedling raising tray, the seedling raising tray is covered with a moisturizing cover, dark treatment is conducted for 24 h, and then light culture is conducted in the environment with the relative humidity being 65-75%; an efficient induction transformation system of the cotton hairy roots under the open condition can be established. The method for rapidly inducing the cotton transgenic hairy roots under the open condition is established, sterile operation is not needed, a culture medium does not need to be used, cotton genotype limitation is avoided, and a simple, convenient and efficient research method is provided for cotton gene function analysis and root system biology research.

The invention belongs to the technical field of space plasma environment detection, and relates to a low-level high-precision detection device for a gated high voltage based on a field-effect transistor, and the device comprises a level processing circuit which is realized based on the field-effect transistor, is used for filtering a high-voltage part of the inputted gated high voltage, and only outputs a low voltage; the ADC conversion circuit is used for periodically outputting gated high-voltage level data after analog-to-digital conversion at a set frequency under the control of the data processing unit; and the data processing unit is realized on the basis of the FPGA and is used for controlling the ADC conversion circuit, collecting level data output by the ADC conversion circuit, packaging the level data and outputting the packaged level data to a PC (Personal Computer) end through an RS422 interface. By adopting the device provided by the invention, the measuring range of the oscilloscope is reduced from 300V to 3V, and the measuring precision is improved from 50V / div to 0.5 V / div, so that the problem of low measuring precision of gating high-voltage high-low level switching is solved, and the risk of high-voltage discharge caused by direct measurement of high voltage by an oscilloscope meter pen is avoided.

The invention provides a test system and a test method for water vapor migration in a soil freezing and thawing process, and the test system is provided with a soil sample loading device, a refrigeration system, a water supplementing system, a traffic load simulation system, an air pressure control system and a data analysis system. The influence of contact water replenishing, non-contact water replenishing and non-water replenishing on water vapor migration in the one-dimensional freezing and thawing process of the soil can be realized; the influence of different temperatures and different freezing times on water vapor migration in the soil freezing and thawing process is researched by adjusting the temperature and the freezing time of the refrigerating device; by adjusting the frequency and the size of the output load, the influence of the traffic load effect on the water vapor migration of the soil body in the soil freezing and thawing process is researched. The device is simple, and research and visual display of the influence of soil moisture migration under different water replenishing conditions, freezing and thawing temperatures, freezing and thawing time and traffic load conditions can be realized.

The present invention discloses a two-dimensional magnetic lattice vibration experimental device and method. It includes an oscillating component, a shooting component and an oscillator mechanism. The oscillator mechanism is provided with a plurality of oscillators with magnets. The oscillating component is used to touch the oscillator to oscillate and conduct a two-dimensional lattice vibration experiment. The shooting component is used to shoot the two-dimensional lattice vibration experiment. The oscillator mechanism includes two different "spring-magnet" oscillators. Each "spring-magnet" oscillator includes a closely wound spring, a columnar hollow magnet and a rubber ring. The upper end of the closely wound spring is provided with a columnar hollow magnet, and the lower end of the closely wound spring is inserted into a square acrylic plate. The present invention is used to "macroscopically" simulate the phonon and lattice wave phenomena in the crystal lattice. By adjusting parameters such as the vibration amplitude and frequency, the physical experiment teaching of the phonon band theory is made more intuitive. By changing the periodicity and spatial structure of the magnetic oscillator, the characteristic differences of the two-dimensional lattice vibration mode and the mechanical wave behavior under different interactions can be shown.

The application discloses a kind of deep water reservoir thermal stratification and water ecological environment response comprehensive simulation device and method, belong to ecological water conservancy experimental technical field.Device includes the main simulation column of the height-diameter ratio greater than 5:1, stratified temperature control module, dissolved oxygen control module, vertical profile monitoring module and host computer.Stratified temperature control module forms the water temperature stratification of upper warm lower cold by upper independent heating unit and lower cooling unit;Dissolved oxygen control module cooperates by surface oxygenation unit and bottom oxygen consumption / deoxidation unit to build the dissolved oxygen gradient of upper high lower low;Vertical profile monitoring module is by the lifting platform of stepping motor drive, carries out vertical physicochemical parameter real-time monitoring by multiple-parameter sensor group;Host computer uniformly dispatches each module operation.The application can accurately reproduce deep water reservoir thermal stratification structure and its ecological effect, provide controllable, repeatable indoor experimental platform for algal bloom growth and consumption, endogenous pollution and greenhouse gas emission mechanism research.

This invention provides a multi-to-multi drug screening method for CRISPRa / i GPCR libraries based on a three-dimensional spatial hybrid screening model and its applications, belonging to the field of biomedical technology. The method includes the following steps: Step (1): Constructing a screening cell pool; Step (2): Classifying the drugs to be screened; Step (3): Constructing a drug hybrid screening model; Step (4): Grouping drugs according to mutually perpendicular X, Y, and Z planes, with drugs in each plane mixed to form independent pools; Step (5): Processing each pool into a cell pool; Step (6): Detecting and analyzing the changes in sgRNA abundance in each pool; Step (7): Decoding to determine the drug-GPCR interaction pairs. This invention offers higher screening throughput, realizes a multi-target, multi-drug screening mode, reduces costs and time, and enables large-scale research on drug-gene interaction networks, which is of great significance for drug research and development.

The invention discloses a component-limited insect culture medium containing nanoparticles, and relates to the technical field of culture medium preparation, and the component-limited insect culture medium is composed of saccharides, amino acids, inorganic salts, vitamins, organic acids and trace elements. The chemical component limited culture medium comprises 10-15 g / L of saccharides, 15-25 g / L of amino acid, 6.5-15.0 g / L of inorganic salt, 0.01-0.5 g / L of vitamins, 0.2-2.0 g / L of organic acid, 0.001-0.01 g / L of trace elements and nano-particles, and the mass of the nano-particles accounts for 0.01%-0.1% of the total mass of the culture medium. The culture medium is free of serum and animal source components, the nano-particles are added into the serum-free culture medium, higher batch stability is achieved, and the culture medium is suitable for large-scale production. The recovery and passage requirements of various insect cells can be met.

The present application relates to the technical field of bridge engineering test, in particular to a model test device and test method for force state of a rotating cable at a rotating cable saddle, the model test device comprising a force applying mechanism, a horizontal turning device, a rotating cable saddle device and an anchoring device, the force applying mechanism being used to connect with one end of a main cable test piece and apply tension to the main cable test piece, the anchoring device being used to fix the other end of the main cable test piece, the horizontal turning device and the rotating cable saddle device being arranged between the force applying mechanism and the anchoring device and being used for winding of the main cable test piece, the horizontal turning device being used to turn the main cable test piece from vertical to horizontal and enter the rotating cable saddle device horizontally and tangentially, and the rotating cable saddle device being used to simulate a rotating cable saddle so that the main cable test piece tangentially leaves the rotating cable saddle device. The present application realizes simulation test of different force states of the main cable of a single-tower single-cable suspension bridge at the rotating cable saddle.

The invention discloses a wheat grain microstructure change observation method and device based on multi-modal fusion imaging, and the method comprises the following steps: S1, inputting the X-ray and infrared modal data of wheat grains into a fusion backbone network, and generating a fusion feature map; s2, establishing a comparison optimization learning model for foreground targets and background details of wheat grain imaging; and S3, constructing an adaptive weight loss constraint function to realize wheat multi-modal image enhancement representation. According to the invention, a multi-modal fusion imaging method is utilized to overcome the defects of single-modal imaging, such as low imaging quality and single imaging channel. And meanwhile, redundant information brought by fusion is reduced by adopting a multi-level feature integration coupling contrast learning method, so that multi-modal image enhancement representation of the wheat grains is realized.

The invention discloses a large natural caving method ore drawing model which comprises a model box body, the model box body is a detachable spliced box body, fixed angle steel stand columns are arranged on the vertical side edges of the box body, and the four corners of a bottom plate of the box body and the angle steel stand columns are embedded and fixed. The vertical edges of four side plates of the box body are respectively spliced and fixed with the angle steel upright posts through detachable connecting pieces, and the bottom edges of the side plates are connected with the side edges of the bottom plate to define the model box body; the bottom plate comprises a back plate for bearing the pressure of ore particles in the model box body, a three-dimensional ore drawing opening is formed in the middle area of the back plate, a plane ore drawing opening is formed along one side edge of the back plate, and a detachable three-dimensional ore drawing baffle and a detachable plane ore drawing baffle are arranged on the bottom plate to seal the three-dimensional ore drawing opening and the plane ore drawing opening respectively. The three-dimensional ore drawing opening or the plane ore drawing opening is opened according to specific experiments to be in butt joint with the ore drawing structure. The ore drawing simulation experiment of the natural caving method can be well achieved, the applicability is wide, practicability is high, and operability is simple.

The invention relates to an explosion testing device and method based on a gravity ballistic pendulum method. The structure of the device mainly comprises an integral frame, a pendulum bob, an energy storage spring, a counterweight, an explosion box body and a displacement sensor, the integral frame comprises a top plate, top plate supporting columns, a middle bearing platform, bottom plate supporting columns and a bottom plate. The pendulum bob comprises a hammer head, four supporting rods and a top disc, the hammer head is connected with the top disc through the supporting rods, a gasket needs to be used when the pendulum bob is installed, and the gasket is installed between the top disc and the middle bearing platform of the integral installation frame. The energy storage spring is mounted at the boundary of the circular boss on the lower end surface of the top plate; the counter weight is mounted on a top disc of the pendulum bob; the explosion box is mounted in the center of the bottom plate; a basic applied displacement sensor is installed in a box body on the lower end face of the top plate. According to the invention, an innovative gravity pendulum explosion test method is matched with damping energy measurement, so that the actual explosion working condition is simulated in an open environment, corresponding data measurement is completed, and the influence of explosion impact on the service safety performance of materials and structures is evaluated.

The invention discloses a two-dimensional magnetic lattice point vibration experiment device and method. Comprising an oscillation starting assembly, a shooting assembly and a vibrator mechanism, a plurality of vibrators with magnets are arranged on the vibrator mechanism, the oscillation starting assembly is used for touching the vibrators to start oscillation for a two-dimensional lattice point vibration experiment, and the shooting assembly is used for shooting the condition of the two-dimensional lattice point vibration experiment; the vibrator mechanism comprises two different spring-magnet vibrators, each spring-magnet vibrator comprises a closely-wound spring, a columnar hollow magnet and a rubber ring, the upper end of the closely-wound spring is sleeved with the columnar hollow magnet, and the lower end of the closely-wound spring is inserted into the square acrylic plate. The device is used for'macroscopically 'simulation of phonons and lattice wave phenomena in crystal lattices, physical experiment teaching of the phonon energy band theory is more intuitive by adjusting parameters such as vibration amplitude and frequency, and the physical experiment teaching of the phonon energy band theory is more intuitive by changing the periodicity and space structure of the magnetic oscillators. And characteristic differences of two-dimensional lattice point vibration modes and mechanical wave behaviors under different interactions can be displayed.

The invention discloses a system and a method for testing the anti-interference electricity tolerance characteristic of a centrifugal pump, and the system comprises a power supply loop, a process loop and a fault recorder which are connected with one another, and the power supply loop comprises an interference electricity simulator, a frequency converter, a motor and a torque sensor. The voltage sag simulator is used for simulating three-phase short circuit, single-phase grounding short circuit faults, two-phase grounding short circuit faults and voltage drop characteristics of the two-phase short circuit faults. The process loop comprises a centrifugal pump, a first pressure gauge, a water tank, a second pressure gauge, an electric control valve and a turbine flowmeter. The influence of different interference electricity depths and time on performance parameters such as shaft power, lift and efficiency when various short circuit faults occur is reflected by detecting the torque change of the motor, the flow change of the centrifugal pump and the pressure change, and the technical response characteristics of the centrifugal pump under the interference electricity working condition are studied and tested to determine the interference electricity of the centrifugal pump. The research on the anti-interference electricity tolerance characteristic of the centrifugal pump is realized.