44 results about "Doxycycline" patented technology

The invention provides an acid-resistant and anti-pollution composite nanofiltration membrane as well as a preparation method and application thereof, and relates to the technical field of membrane materials. The preparation method comprises the following steps: soaking the surface of a support membrane with a mixed solution I; the mixed solution I is prepared from doxycycline, 4-aminopyridine and deionized water; pouring an oil phase solution into the surface of the soaked support membrane to carry out interfacial polymerization reaction; the oil phase solution is composed of 1, 3-benzene disulfonyl chloride and cyclohexane; and carrying out heat treatment on the support membrane after the interfacial polymerization reaction to obtain the acid-resistant anti-pollution composite nanofiltration membrane. According to the prepared composite nanofiltration membrane, doxycycline is selected as a water-phase monomer for the first time, meanwhile, 1, 3-benzene disulfonyl chloride is selected as an oil-phase monomer, 4-aminopyridine is selected as an additive, and the prepared composite nanofiltration membrane not only has acid resistance and pollution resistance, but also has high permeation flux and high separation performance.

The application provides a multifunctional nano particle for targeting abdominal aortic aneurysm and a preparation method and application thereof, and belongs to the field of nanobiomedicine, wherein polyphenol oxidation self-polymer nanoparticles are used as carriers, doxycycline is loaded, and a targeting ligand cRGD is modified on the surface of the nano carrier; the neovasculature in the media and adventitia of AAA sites helps accumulation of the nanoparticles in the abdominal aortic aneurysm, and the integrin αν3 beta receptor highly expressed on the surface of the diseased cell membrane can recognize the cRGD with high affinity, and then mediate the targeted endocytosis of the nanoparticles; after intravenous injection, the nanoparticles can be effectively enriched in the lesion site and achieve long-term retention, and can release DC in response to the high ROS level in the tumor microenvironment; the drug is rapidly released to take effect, and the non-specific toxic side effects are reduced; the free radical scavenging capacity of the nanoparticles can be synergized with the DC activity to treat AAA through multiple mechanisms such as anti-inflammatory, antioxidant, macrophage repolarization promotion, anti-apoptosis and calcification inhibition, and MMPs inhibition.

The invention discloses a construction and verification method of an HEK293T cell line capable of stably overexpressing human ANT2, which comprises the following steps: cloning a human ANT2 gene, constructing the human ANT2 gene into a lentivirus expression plasmid pLVX-TRE3G, co-transfecting the lentivirus expression plasmid pLVX-TRE3G and a helper plasmid pLVX-Tet3G into an HEK293T-ACE2 cell, and carrying out puromycin and G418 pressurized screening and Western blot identification to obtain the HEK293T cell line capable of stably overexpressing human ANT2. A result shows that the expression quantity of the ANT2 is the highest when the HEK293T-ACE2-ANT2 cell is induced for 12 hours at the Doxycycline of 6mg / L; when the ANT2 is induced to express, the virus nucleic acid load and the N protein expression level after SARS-CoV-2 infection are obviously reduced, which indicates that the ANT2 has an inhibition effect on SARS-CoV-2 infection replication, and a cell model and an experimental basis are provided for exploring the effect of the ANT2 in virus infection resistance.

The present invention relates to nucleic acids and modified immune cells or precursors thereof, comprising a first polynucleotide encoding an NF-κB p65-rTetO fusion protein comprising one or more transactivation domains (TAD) of human NF-κB p65 fused to a reverse Tet operator (rTetO) binding domain; a second polynucleotide comprising a Tet operator region (TetOR) for providing inducible expression of one or more transgene(s) operatively linked thereoto; and a third polynucleotide comprising a promoter directing transcription of p65-rTetO from a first strand of DNA, where the p65-rTetO is configured to induce transcription of a transgene of interest operatively linked to the TetOR in the presence of tetracycline or doxycycline (Dox) from an opposite strand of DNA.

The invention provides a preparation method and application of a doxycycline (DOX) molecularly imprinted surface plasmon resonance (SPR) sensing chip. According to the chip, an SPR bare gold chip is used as a substrate, DOX is used as a template molecule, a molecularly imprinted polymer (MIP) recognition membrane is constructed on the surface of the chip, and collaborative integration of the MIP technology and the SPR technology is achieved. According to the strategy, the chip is endowed with specific recognition capability on template molecules DOX, and local refractive index change caused by combination of the DOX and the recognition film can be detected in real time in an unmarked manner, so that the detection sensitivity and accuracy are remarkably improved. By utilizing an SPR (Surface Plasmon Resonance) analyzer, the chip can realize rapid quantitative detection of DOX in a low concentration range, shows good reproducibility, and can be applied to detection of actual samples.

The present application relates to a kind of pluripotent stem cell co-differentiation method for generating vascularized heart organoids, the method comprises: (1) in iPSCs, obtain iEC-iPSCs by transferring exogenous gene Tet 3G and ETV2;(2) the iEC-iPSCs in step (1) are mixed with iPSCs, add culture medium I, prepare blastula after culture;(3) the blastula in step (2) is induced by using the heart organoid differentiation method combined with doxycycline, and vascularized heart organoids are prepared after differentiation culture.The present application uses Tet-on 3G inducible expression system, promotes the differentiation of part of cells in heart organoids to endothelial cell lineage by regulating the expression of ETV2 gene in part of iPSCs in blastula, and then forms vascularized heart organoids.

The invention discloses a nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1) with a capability of degrading multiple pollutants and an application of the nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1, and belongs to the technical field of screening and application of functional microorganisms. The strain is derived from a chicken manure aerobic composting environment, the preservation number is GDMCC No: 67351, and nitro polycyclic aromatic hydrocarbons, polycyclic aromatic hydrocarbons and tetracycline antibiotics can be efficiently degraded at the same time. In a single pollutant with the concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceed 97% in 8 h, the degradation rate of nitrofluoranthene reaches 99.01% in 48 h, and the degradation rate of phenanthrene reaches 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the 48-hour degradation rate is kept to be 78% or above. The strain is high in degradation efficiency and strong in environmental adaptability, provides a new microbial resource for bioremediation of a combined pollution site, and has a wide application prospect.

The application discloses a complex preparation containing bovine lactoferrin and lyticase and application thereof, and belongs to the technical field of biology. The preparation is composed of bovine lactoferrin and streptococcal lyticase, and both are prepared through a baculovirus-insect cell eukaryotic expression system. Researches show that the complex preparation has a significant synergistic antibacterial effect. The complex preparation not only can effectively inhibit a plurality of gram-positive bacteria, but also can overcome the limitation that single streptococcal lyticase has weak activity on gram-negative bacteria, and significantly enhances the inhibiting effect on escherichia coli and salmonella. Especially, the complex preparation still has clear in-vitro antibacterial activity on multi-drug resistant escherichia coli such as ciprofloxacin and doxycycline, thereby providing a new way for developing a new type of drug for resisting drug-resistant bacteria.

The present application relates to the technical field of pharmaceutical analysis, and specifically discloses a method for detecting the purity of doxycycline for injection. The present application mainly solves the problems caused by ion pair reagent and high-salt buffer in the existing chromatographic method, such as serious pollution of the system pipeline and the chromatographic column, difficulty in connecting with mass spectrometry for impurity structure identification, and insufficient separation degree of key polar impurities such as 4-epi doxycycline. The present application purifies the sample by physical dilution and filtration, and prepares uniform aerosol by ultrasonic atomization. The ion mobility spectrometry technology is adopted to realize the efficient separation of gas-phase ions under a weak electric field according to the difference in ion collision cross section (CCS) value, and the epimer which is difficult to distinguish by traditional methods can be excellently separated. The multi-modal sensor is used to synchronously collect ultraviolet, fluorescent and terahertz multi-dimensional optical signals. Finally, based on the intrinsic physical parameter database, the intelligent analysis is carried out by using the machine learning algorithm, and the qualitative and quantitative results are automatically output.

Provided herein are methods of treating a neurodegenerative disorder (e.g., Parkinson's disease) or symptoms thereof in a patient by administering to the patient doxycycline (or a pharmaceutically acceptable salt thereof), either alone or in combination with other therapeutic agents (e.g., levodopa).

This invention discloses a strain of *Pseudomonas nicosulfuron-methyl* capable of degrading multiple pollutants. Pseudomonas nicosulfuronedens HD1 and its applications belong to the field of functional microbial screening and application technology. This strain, originating from an aerobic composting environment of chicken manure (accession number GDMCC No:67351), can simultaneously and efficiently degrade nitropolycyclic aromatic hydrocarbons (NPH), polycyclic aromatic hydrocarbons (PAHs), and tetracycline antibiotics. In single pollutants at a concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceeded 97% after 8 hours; the degradation rate of nitrofluoranthene reached 99.01% after 48 hours; and the degradation rate of phenanthrene reached 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the degradation rate remained above 78% after 48 hours. This strain exhibits high degradation efficiency and strong environmental adaptability, providing a new microbial resource for the bioremediation of multi-contaminated sites and possessing broad application prospects.

Disclosed are a method for screening a riboswitch capable of specifically recognizing doxycycline as well as rational engineering and application of the riboswitch, and belongs to the field of gene expression regulation. The present disclosure provides a doxycycline specific riboswitch, and establishes a high-throughput screening method based on flow cytometry, and obtains the doxycycline riboswitch with further improved activation fold by way of computer-aided prediction of binding sites of the riboswitch and calculation of mutation sites. According to a dose-response curve, the riboswitch shows linear correlation at 40-100 μg / L, and meanwhile, a whole-cell sensor constructed is low in price, high in stability and simple to operate, and can be used for on-site rapid detection.

The invention discloses a p16-OSKM transgenic mouse model and a reprogramming senescence cell construction method and application, and belongs to the technical field of cell biology and regenerative medicine. According to the method, a p16-OSKM hybrid mouse capable of specifically inducing an OSKM factor in a p16 high expression cell is obtained on the basis of hybridization of a p16-Ink4a-CreERT2 transgenic mouse, a Rosa26-CAG-LSL-rtTA3-IRES-mKate2 transgenic mouse and a Col1a1-TRE-OSKM-IRES-mCherry transgenic mouse. The selective activation of the OSKM factor in senescent cells with high expression of p16 can be realized by adding doxycycline into primary embryo fibroblasts separated from the mouse under an in-vitro culture condition, so that the senescent cells enter a cell cycle again and recover division and multiplication capacities. The technology provides an accurate model platform for basic research, and can be used for aging mechanism exploration, cell fate regulation and control, chronic disease treatment, development and screening of anti-aging drugs and the like.

The application belongs to the field of materials and water treatment, and provides a preparation of lanthanum-containing schroeckingerite material and application of the material in antibiotic wastewater treatment, comprising: adding schroeckingerite to an alkali solution, then adding a La salt, performing a reaction, aging, collecting a precipitate, washing, drying, grinding, and obtaining the lanthanum-containing schroeckingerite material; the mass ratio of the schroeckingerite to the La salt is (1-2):(0.147-1.47). The lanthanum-containing schroeckingerite material can quickly remove antibiotic residues in water, has a simple preparation method, low cost, and a wide source of raw materials, and has a good application prospect. The material does not produce secondary pollution in the process of treating environmental pollutants, and is friendly to the environment. In the absence of external energy input, the lanthanum-containing schroeckingerite material can remove more than 90% of doxycycline, and is obviously superior to ordinary chemical synthesis of schroeckingerite.

The application provides a strain of Gilmaniella vishniacov Sarocladium kiliense ) 19-11C and an application thereof, preservation number of the Gilmaniella vishniacov Sarocladium kiliense ) 19-11C is CGMCC No.41846. As a fungus, the Gilmaniella vishniacov 19-11C is naturally insensitive to tetracycline antibiotics, can survive and play a degradation function in a high-concentration drug environment. The strain does not carry horizontally transferable bacterial drug resistance genes, fundamentally avoiding the potential environmental risk of causing drug resistance gene spread in the repair process. The strain shows wide adaptability and stability to changes in temperature, pH and nutritional conditions in the environment, effectively decomposes doxycycline through various biochemical pathways such as removal and oxidation, significantly reduces the biological toxicity, and provides an environmentally friendly and ecologically safe biological solution for the residue treatment of tetracycline antibiotics in the environment.

The invention discloses a PINK1 inducible expression cell line of a porcine umbilical vein endothelial cell and a construction method and application thereof, a stable cell line with controllable PINK1 expression level is constructed by introducing a porcine PINK1 gene into the porcine umbilical vein endothelial cell and combining with an inducible expression system taking doxycycline as an inducer. The cell line is in a low-expression or non-expression state under the condition that an inducer is not added, and dose-dependent induced expression can be realized after doxycycline is added. Experimental results show that the induced expression of PINK1 can regulate and control the expression of angiogenesis related genes, activate mitochondrial autophagy related pathways under oxidative stress conditions, and influence the expression of cell proliferation and apoptosis related proteins at the same time. The cell line is clear in construction method and good in stability, can be used as an in-vitro model for researching angiogenesis, oxidative stress and related regulation and control mechanisms of porcine vascular endothelial cells, and has good practical value.

The invention belongs to the technical field of biology, and discloses an influenza B virus sensitizing cell strain as well as a construction method and application thereof. The influenza B virus sensitizing cell strain STX-BB2 can be used for stably expressing influenza virus receptor ST6GAL1 protein in a human source and controllably inducing and expressing influenza B virus PB2 protein by using doxycycline; the preservation number of the cell strain STX-BB2 is GDMCC (China General Microbiological Culture Collection Center) NO: 67100; the cell strain can enhance the expression ability of a host receptor and the virus replication key gene level at the same time; the infection efficiency and the replication titer of the influenza B virus in MDCK cells can be improved; the method can be widely applied to influenza B virus separation, mechanism research, vaccine seed virus amplification and drug screening model construction.

Disclosed herein are injectable compositions, such as nonaqueous liquid injectable compositions, comprising at least one tetracycline antibiotic, such as doxycycline, and at least one semifluorinated alkane, such as 1-perfluorohexyloctane (F6H8), as well as methods of treatment or prevention of malar edema, with such compositions. The injectable compositions may further be provided in a container closure system, such as a polypropylene (PP) pre-filled syringe, or in a kit.

The invention discloses a silver nanocluster with red emission and detection and antibacterial properties thereof, the silver nanocluster is prepared by adopting dihydrolipoic acid as a ligand and sodium borohydride as a reducing agent through an optimized synthesis process, the quantum yield is as high as 14.8%, the fluorescence lifetime is 7.91 microseconds, and the silver nanocluster has excellent fluorescence characteristics and stability. High-selectivity detection of the heavy metal ion Ni < 2 + > can be realized based on a fluorescence resonance energy transfer mechanism, and the detection limit is as low as 68 nM. Meanwhile, four types of tetracycline antibiotics (TCs) of oxytetracycline (OTC), tetracycline (TC), doxycycline (DOX) and aureomycin (CTC) are recognized through specific ratio fluorescence response. The compound has an efficient inhibition effect on common pathogenic bacteria such as gram-positive bacteria and gram-negative bacteria, and the bacteriostasis rate reaches 80% or above. The hydrogel dressing prepared by compounding AgNCs and a natural polymer material sodium alginate can effectively inhibit wound infection and promote tissue regeneration and wound healing.

The invention relates to the field of cell engineering, and particularly discloses a preparation method and application of a conditionally immortalized human myocardial fibroblast model. According to the method, a Tet-on system driven by a cardiac fibroblast specific promoter is constructed to regulate and control a lentiviral vector of an SV40LT-tsA58 temperature-sensitive mutant gene, and after primary human cardiac fibroblasts are transduced, conditional immortalization of the cells is realized in the presence of an inducer doxycycline. The model can be stably amplified under an induction condition, the immortalized gene expression is closed after an inducer is removed, the primary physiological state of cells is recovered, and the high-fidelity response capability to fibrosis promoting and anti-fibrosis drugs is reserved. According to the method, the in-vitro proliferation limit of primary cells is broken through, the problems of species difference and ethics are avoided, and a humanized, high-fidelity and high-controllability standardized tool is provided for myocardial fibrosis pathological mechanism research and drug screening.

The invention discloses a molecularly imprinted magnetic covalent organic framework as well as a preparation method and application thereof, and relates to the technical field of functional materials. The preparation method comprises the following steps: performing prepolymerization reaction on template molecules and functional monomers in a pore-foaming agent, adding a magnetic metal covalent organic framework for mixing reaction, then adding a cross-linking agent and an initiator for polymerization reaction, separating out a product under the action of an external magnetic field after the reaction is finished, and eluting to remove the template molecules, thereby obtaining the magnetic metal covalent organic framework. The molecularly imprinted magnetic covalent organic framework is obtained; the template molecule is doxycycline. The molecularly imprinted magnetic covalent organic framework has the advantages of efficient magnetic separation and high selectivity, and can simply, stably and reliably detect DOX in a complex sample.

The application provides a Citrobacter murliniae strain DH3 and application thereof in adsorbing heavy metals and / or degrading doxycycline, and belongs to the technical field of environmental microorganisms. The application provides a Citrobacter murliniae strain DH3, and biological preservation has been completed. The Citrobacter murliniae strain DH3 can tolerate various heavy metals including Cu 2+ , Pb 2+ , Cr 6+ , Cd 2+ , has high removal capacity for Pb 2+ and / or Cd 2+ , and has high degradation capacity for doxycycline. Tests find that the Citrobacter murliniae strain DH3 can tolerate the compound pollution conditions of heavy metals and doxycycline, and can achieve the effect of simultaneously removing heavy metals and degrading doxycycline, thereby providing new strain resources for the treatment of aquaculture water pollution.

The invention provides a strain of Sarcocladium kiliense 19-11C and application of the Sarcocladium kiliense 19-11C, and the preservation number of the Sarcocladium kiliense 19-11C is CGMCC (China General Microbiological Culture Collection Center) No. 41846. The Sarcocladium kiliense 19-11C can be used for preparing the Sarcocladium kiliense 19-11C. As a fungus, the strain is natural and insensitive to tetracycline antibiotics, and can survive in a high-concentration drug environment and play a degradation function. The strain does not carry bacterial drug-resistant genes which can be horizontally transferred, so that the potential environmental risk of drug-resistant gene diffusion caused in the repairing process is fundamentally avoided. The strain shows wide adaptability and stability to changes of temperature, pH and nutritional conditions in the environment, the doxycycline is effectively decomposed through various biochemical ways such as removal and oxidation, the biotoxicity of the doxycycline is remarkably reduced, and an environment-friendly and ecologically safe biological solution is provided for treatment of tetracycline antibiotic residues in the environment.

A reliable and convenient method is provided for achieving precise control of gene expression in the liver in vivo. According to the method, a doxycycline inducible gene expression system is generally combined with a sleep beauty transposon carrier and fluid dynamic injection, so that a controllable gene expression system can be effectively integrated into a genome of a hepatocyte. Doxycycline inducible systems generally allow for precise and reversible control of gene expression. In some forms, the combination of genetic alterations and Myc overexpression results in the development of HCC in animals. The methods may also use vectors that allow for inducible expression of tumor antigens. The method also can induce tumor antigen specific T cell immune response, and provides a powerful tool for developing a targeted therapy for HCC.

The present disclosure relates in some aspects to compositions, such as pharmaceutical compositions for topical administration, which are useful for treating or preventing hair loss, hair graying, and dermatological conditions in a subject, such as comprising a tetracycline, for example doxycycline. Also provided are kits comprising the compositions, and methods of using the compositions for treating or preventing hair loss, hair graying, and dermatological conditions.

Disclosed herein are injectable compositions, such as nonaqueous liquid injectable compositions, comprising at least one tetracycline antibiotic, such as doxycycline, and at least one semifluorinated alkane, such as 1-perfluorohexyloctane (F6H8), as well as methods of treatment or prevention of malar edema, with such compositions. The injectable compositions may further be provided in a container closure system, such as a polypropylene (PP) pre-filled syringe, or in a kit.

The invention provides nitrate reducing bacteria C381 as well as a method and application thereof. The nitrate reducing bacteria C381 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36842; the invention provides a novel oral cavity probiotic nitrate reducing bacteria C381, the nitrate reducing bacteria C381 has an inhibition effect on periodontal disease bacteria, and the nitrate reducing bacteria C381 is sensitive to antibiotics such as erythromycin, piperacillin, minocycline, doxycycline, cefazolin, tetracycline, amikacin, gentamicin, vancomycin and cefoperazone, and can be used for preparing oral cavity probiotics for treating periodontal disease bacteria. And the product has good survival ability in the oral cavity.

The invention discloses a bidirectional selective fluorescence detection method for doxycycline and Sc < 3 + > in an environmental sample, and belongs to the technical field of environmental monitoring. The method comprises the following steps: S1, mixing a sensing material aqueous solution with a pretreated environmental sample to form a detection system, and then adjusting the pH value of the detection system to 2; s2, reacting the pH-adjusted detection system at room temperature, detecting the fluorescence intensity at 567 nm under the excitation wavelength of 380 nm, and realizing quantitative detection based on the relationship between the fluorescence intensity and the concentration of a target object; wherein when the sensing material is Sc < 3 + >, the target object is DC; when the sensing material is DC, the target object is Sc < 3 + >, and the two detection modes have no sequence. According to the invention, when the pH value of a detection system is 2, an exclusive coordination reaction can be carried out between DC and Sc < 3 + >, so that the fluorescence emission of DC at 567 nm is specifically sensitized and enhanced, and other tetracycline antibiotics and rare earth ions cannot trigger the specific reaction, thereby avoiding interference.

The application provides a digoxin microtablet and a preparation method thereof. The digoxin microtablet prepared by the application contains 5-100 micrograms of digoxin per tablet, which can meet the dosage requirements of patients of different ages, realize precise dosage administration, and reduce the risk of drug overdose in clinical application. The digoxin microtablet provided by the application can solve the problem that infants and children have difficulty in swallowing the medicine, thereby improving the medication compliance of infants and children. Compared with conventional tablets, the microtablet has a smaller volume. The digoxin microtablet provided by the application is prepared by optimizing the prescription and process, preparing a digoxin compound by a spray drying and hot melt extrusion technology, obtaining a solid dispersion, and having better content uniformity and dissolution rate, and significantly improving the bioavailability.

The invention relates to the technical field of fluorescence sensing and the field of antibiotic fluorescence detection, in particular to a cadmium (II) MOFs (Metal-Organic Frameworks) material as well as a preparation method and application thereof in antibiotic detection. A cadmium (II) MOFs material [Cd (cpt) 2H2O] n can be prepared by adopting a one-pot hydrothermal reaction, the molecular formula is C56H38CdN6O7, and Hcpt is 4 '-(4-(4-carboxyl phenoxy) phenyl)-4, 2': 6 ', 4' '-terpyridyl. The preparation method has the advantages of mild conditions, simplicity and convenience in operation, high yield, good repeatability and the like. The cadmium (II) MOFs material in the prepared cadmium element coordination polymer has good structural stability and fluorescence characteristics and shows high selectivity and sensitivity to tetracycline antibiotics such as tetracycline hydrochloride, aureomycin and doxycycline (THC, CTC and DOX) in antibiotic detection, and the concentration of the tetracycline antibiotics can be preferentially detected through a fluorescence quenching phenomenon.