100 results about "Doxycycline" patented technology

The invention discloses a nucleic acid aptamer TET-HeR of tetracycline and application of the nucleic acid aptamer TET-HeR, and belongs to the technical field of nucleic acid aptamers. The nucleic acid aptamer TET-HeR of the tetracycline is a combination of an aptamer TET-A1 and an aptamer TET-C1, the nucleotide sequence of the aptamer TET-A1 is as shown in SEQ ID NO.5, and the nucleotide sequence of the aptamer TET-C1 is as shown in SEQ ID NO.7. The nucleic acid aptamer TET-HeR of the tetracycline is a combination of the aptamer TET-A1 and the aptamer TET-C1. The invention further discloses the application of the nucleic acid aptamer TET-HeR of the tetracycline in detection of the tetracycline. The affinity and dissociation constant of the nucleic acid aptamer TET-HeR of the tetracycline and the tetracycline is 62.7 nM, and the nucleic acid aptamer TET-HeR of the tetracycline has high affinity; the compound has no obvious affinity to tetracycline analogues such as aureomycin and doxycycline, has weak affinity to oxytetracycline, and has good specificity; the method can be used for specific detection of tetracycline.

The invention provides an acid-resistant and anti-pollution composite nanofiltration membrane as well as a preparation method and application thereof, and relates to the technical field of membrane materials. The preparation method comprises the following steps: soaking the surface of a support membrane with a mixed solution I; the mixed solution I is prepared from doxycycline, 4-aminopyridine and deionized water; pouring an oil phase solution into the surface of the soaked support membrane to carry out interfacial polymerization reaction; the oil phase solution is composed of 1, 3-benzene disulfonyl chloride and cyclohexane; and carrying out heat treatment on the support membrane after the interfacial polymerization reaction to obtain the acid-resistant anti-pollution composite nanofiltration membrane. According to the prepared composite nanofiltration membrane, doxycycline is selected as a water-phase monomer for the first time, meanwhile, 1, 3-benzene disulfonyl chloride is selected as an oil-phase monomer, 4-aminopyridine is selected as an additive, and the prepared composite nanofiltration membrane not only has acid resistance and pollution resistance, but also has high permeation flux and high separation performance.

The application provides a multifunctional nano particle for targeting abdominal aortic aneurysm and a preparation method and application thereof, and belongs to the field of nanobiomedicine, wherein polyphenol oxidation self-polymer nanoparticles are used as carriers, doxycycline is loaded, and a targeting ligand cRGD is modified on the surface of the nano carrier; the neovasculature in the media and adventitia of AAA sites helps accumulation of the nanoparticles in the abdominal aortic aneurysm, and the integrin αν3 beta receptor highly expressed on the surface of the diseased cell membrane can recognize the cRGD with high affinity, and then mediate the targeted endocytosis of the nanoparticles; after intravenous injection, the nanoparticles can be effectively enriched in the lesion site and achieve long-term retention, and can release DC in response to the high ROS level in the tumor microenvironment; the drug is rapidly released to take effect, and the non-specific toxic side effects are reduced; the free radical scavenging capacity of the nanoparticles can be synergized with the DC activity to treat AAA through multiple mechanisms such as anti-inflammatory, antioxidant, macrophage repolarization promotion, anti-apoptosis and calcification inhibition, and MMPs inhibition.

The invention discloses a construction and verification method of an HEK293T cell line capable of stably overexpressing human ANT2, which comprises the following steps: cloning a human ANT2 gene, constructing the human ANT2 gene into a lentivirus expression plasmid pLVX-TRE3G, co-transfecting the lentivirus expression plasmid pLVX-TRE3G and a helper plasmid pLVX-Tet3G into an HEK293T-ACE2 cell, and carrying out puromycin and G418 pressurized screening and Western blot identification to obtain the HEK293T cell line capable of stably overexpressing human ANT2. A result shows that the expression quantity of the ANT2 is the highest when the HEK293T-ACE2-ANT2 cell is induced for 12 hours at the Doxycycline of 6mg / L; when the ANT2 is induced to express, the virus nucleic acid load and the N protein expression level after SARS-CoV-2 infection are obviously reduced, which indicates that the ANT2 has an inhibition effect on SARS-CoV-2 infection replication, and a cell model and an experimental basis are provided for exploring the effect of the ANT2 in virus infection resistance.

The present invention relates to nucleic acids and modified immune cells or precursors thereof, comprising a first polynucleotide encoding an NF-κB p65-rTetO fusion protein comprising one or more transactivation domains (TAD) of human NF-κB p65 fused to a reverse Tet operator (rTetO) binding domain; a second polynucleotide comprising a Tet operator region (TetOR) for providing inducible expression of one or more transgene(s) operatively linked thereoto; and a third polynucleotide comprising a promoter directing transcription of p65-rTetO from a first strand of DNA, where the p65-rTetO is configured to induce transcription of a transgene of interest operatively linked to the TetOR in the presence of tetracycline or doxycycline (Dox) from an opposite strand of DNA.

The invention discloses a core-shell structure microneedle patch loaded with tetracycline antibacterial drugs and simvastatin as well as a preparation method and application of the core-shell structure microneedle patch, and belongs to the field of biomedical materials. An antibacterial drug doxycycline is loaded on a shell structure of a substrate layer and a needle body of the microneedle patch, and a core structure contains simvastatin-loaded polydopamine nanoparticles. The microneedle patch can release antibacterial drugs in stages, and the number of bacteria at local parts of a focus can be rapidly reduced in a short time through burst release of the antibacterial drugs in the first stage, so that inflammation spreading is controlled; in the second stage, the antibacterial medicine is slowly released, so that the formation of a new plaque biofilm is inhibited for a long time, meanwhile, simvastatin is slowly released, the effect of locally promoting osteogenesis is achieved, and the synergistic effect of antibiosis and bone regeneration is achieved. The compound has a good application prospect in the treatment of chronic periodontitis, especially intractable periodontitis combined with systemic diseases, such as chronic periodontitis combined with diabetes mellitus.

The invention provides a preparation method and application of a doxycycline (DOX) molecularly imprinted surface plasmon resonance (SPR) sensing chip. According to the chip, an SPR bare gold chip is used as a substrate, DOX is used as a template molecule, a molecularly imprinted polymer (MIP) recognition membrane is constructed on the surface of the chip, and collaborative integration of the MIP technology and the SPR technology is achieved. According to the strategy, the chip is endowed with specific recognition capability on template molecules DOX, and local refractive index change caused by combination of the DOX and the recognition film can be detected in real time in an unmarked manner, so that the detection sensitivity and accuracy are remarkably improved. By utilizing an SPR (Surface Plasmon Resonance) analyzer, the chip can realize rapid quantitative detection of DOX in a low concentration range, shows good reproducibility, and can be applied to detection of actual samples.

The present application relates to a kind of pluripotent stem cell co-differentiation method for generating vascularized heart organoids, the method comprises: (1) in iPSCs, obtain iEC-iPSCs by transferring exogenous gene Tet 3G and ETV2;(2) the iEC-iPSCs in step (1) are mixed with iPSCs, add culture medium I, prepare blastula after culture;(3) the blastula in step (2) is induced by using the heart organoid differentiation method combined with doxycycline, and vascularized heart organoids are prepared after differentiation culture.The present application uses Tet-on 3G inducible expression system, promotes the differentiation of part of cells in heart organoids to endothelial cell lineage by regulating the expression of ETV2 gene in part of iPSCs in blastula, and then forms vascularized heart organoids.

The invention discloses a strain for efficiently degrading doxycycline in a water body and application of the strain. The Sphingobacterium hotanense DX14 screened by the invention can be used for efficiently degrading the doxycycline in the water body, so that the strain has the potential of being developed into a microbial agent for degrading the doxycycline in the water body, and has a good application prospect for solving the problems that the doxycycline in the antibiotic-containing water body exceeds the standard and the like. Meanwhile, the invention further provides an environment-friendly application method of the sphingobacterium mesophilum DX14, the sphingobacterium mesophilum DX14 is recovered, and genome DNA of the sphingobacterium mesophilum DX14 is completely degraded, so that the doxycycline resistance gene carried by the sphingobacterium mesophilum DX14 is effectively prevented from being diffused in a water body, and the yield of the doxycycline resistance gene is improved. And the effect of comprehensively protecting the environment is achieved.

The invention discloses a nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1) with a capability of degrading multiple pollutants and an application of the nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1, and belongs to the technical field of screening and application of functional microorganisms. The strain is derived from a chicken manure aerobic composting environment, the preservation number is GDMCC No: 67351, and nitro polycyclic aromatic hydrocarbons, polycyclic aromatic hydrocarbons and tetracycline antibiotics can be efficiently degraded at the same time. In a single pollutant with the concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceed 97% in 8 h, the degradation rate of nitrofluoranthene reaches 99.01% in 48 h, and the degradation rate of phenanthrene reaches 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the 48-hour degradation rate is kept to be 78% or above. The strain is high in degradation efficiency and strong in environmental adaptability, provides a new microbial resource for bioremediation of a combined pollution site, and has a wide application prospect.

The invention discloses a Brucella strain and application of the Brucella strain in repairing tetracycline antibiotic pollution. In the process of domesticating and culturing activated sludge collected from a sewage treatment plant, a Brucella strain which is resistant to salt and alkali and capable of degrading tetracycline is obtained, is named as a WSW-Y3 strain and is preserved in Guangdong Microbial Culture Collection Center on May 21, 2025, and the preservation number is GDMCC NO: 66368. In addition to tetracycline degradation, the WSW-Y3 strain can also degrade oxytetracycline and doxycycline, tetracycline antibiotic residues in the environment can be degraded by using the WSW-Y3 strain, and tetracycline antibiotic pollution can be repaired. In addition, the WSW-Y3 strain is loaded on the biochar to prepare the carbon-based microbial agent, so that the degradation rate of tetracycline is further improved. The method is beneficial to repair of tetracycline antibiotic pollution.

The application discloses a complex preparation containing bovine lactoferrin and lyticase and application thereof, and belongs to the technical field of biology. The preparation is composed of bovine lactoferrin and streptococcal lyticase, and both are prepared through a baculovirus-insect cell eukaryotic expression system. Researches show that the complex preparation has a significant synergistic antibacterial effect. The complex preparation not only can effectively inhibit a plurality of gram-positive bacteria, but also can overcome the limitation that single streptococcal lyticase has weak activity on gram-negative bacteria, and significantly enhances the inhibiting effect on escherichia coli and salmonella. Especially, the complex preparation still has clear in-vitro antibacterial activity on multi-drug resistant escherichia coli such as ciprofloxacin and doxycycline, thereby providing a new way for developing a new type of drug for resisting drug-resistant bacteria.

The invention belongs to the field of materials and water treatment, and provides preparation of a lanthanum-containing Schwertmannite material and application of the lanthanum-containing Schwertmannite material in antibiotic wastewater treatment.The preparation method comprises the steps that Schwertmannite is added into an alkali solution, La salt is added, and the lanthanum-containing Schwertmannite material is obtained through reaction, aging, sediment collection, washing, drying and grinding; the mass ratio of the Schwertmannite to the La salt is (1-2): (0.147-1.47). The lanthanum-containing Schwertmannite material can rapidly remove antibiotic residues in water, the preparation method is simple, the cost is low, the used raw materials are wide in source, and the lanthanum-containing Schwertmannite material has good application prospects. The material does not generate secondary pollution in the process of treating environmental pollutants, and is environment-friendly. Under the condition of no external energy input, the lanthanum-containing Schwertmannite material provided by the invention can remove more than 90% of doxycycline, and is obviously superior to the Schwertmannite synthesized by a common chemical method.

The present application relates to the technical field of pharmaceutical analysis, and specifically discloses a method for detecting the purity of doxycycline for injection. The present application mainly solves the problems caused by ion pair reagent and high-salt buffer in the existing chromatographic method, such as serious pollution of the system pipeline and the chromatographic column, difficulty in connecting with mass spectrometry for impurity structure identification, and insufficient separation degree of key polar impurities such as 4-epi doxycycline. The present application purifies the sample by physical dilution and filtration, and prepares uniform aerosol by ultrasonic atomization. The ion mobility spectrometry technology is adopted to realize the efficient separation of gas-phase ions under a weak electric field according to the difference in ion collision cross section (CCS) value, and the epimer which is difficult to distinguish by traditional methods can be excellently separated. The multi-modal sensor is used to synchronously collect ultraviolet, fluorescent and terahertz multi-dimensional optical signals. Finally, based on the intrinsic physical parameter database, the intelligent analysis is carried out by using the machine learning algorithm, and the qualitative and quantitative results are automatically output.

The invention belongs to the field of medicines, and particularly relates to a valnemulin long-acting compound preparation as well as a preparation method and application thereof. The valnemulin long-acting compound preparation comprises the following components in percentage by mass: 5%-10% of valnemulin, 10%-30% of doxycycline, 0.5%-2.0% of tartaric acid, 10%-20% of an oil phase, 10%-30% of an emulsifier, 2%-10% of a co-emulsifier and the balance of water. The nano-emulsion is prepared from valnemulin, doxycycline and corresponding auxiliary materials, so that the release rate of valnemulin can be effectively delayed, the two medicines can be synchronously eliminated in vivo, and a synergistic antibacterial effect is achieved; moreover, the drug is wrapped in the core of the nanoemulsion, so that the solubility of the valnemulin in water is improved, the doxycycline is prevented from forming an insoluble complex with metal ions in water, and the stability of the drug is improved.

The present invention discloses a method for screening a riboswitch that specifically recognizes doxycycline, as well as its rational modification and application, relating to the field of gene expression regulation. The invention provides a doxycycline-specific riboswitch and establishes a high-throughput screening method based on flow cytometry. Computer-assisted prediction of riboswitch binding sites and calculation of mutation sites are used to obtain a doxycycline riboswitch with a further enhanced activation factor. Dose-response curves show that the riboswitch exhibits a linear correlation at 40-100 μg / L. The constructed whole-cell sensor is low-cost, highly stable, and simple to operate, making it suitable for rapid on-site detection.

Provided herein are methods of treating a neurodegenerative disorder (e.g., Parkinson's disease) or symptoms thereof in a patient by administering to the patient doxycycline (or a pharmaceutically acceptable salt thereof), either alone or in combination with other therapeutic agents (e.g., levodopa).

The invention discloses a Chinese monad strain capable of degrading tetracycline antibiotics and application of the Chinese monad strain. The Chinese monad strain is preserved in Guangdong Microbial Culture Collection Center on May 21, 2025, the taxonomic name of the Chinese monad strain is Sinomonas soli, and the preservation number of the Chinese monad strain is GDMCC NO: 66366. The Chinese monad strain has good environmental adaptability, can effectively degrade tetracycline antibiotics such as tetracycline, oxytetracycline, doxycycline and the like, can be used for degrading tetracycline antibiotic residues in the environment, and reduces adverse effects of the tetracycline antibiotics on the ecological environment. The method is beneficial to development of preparations for degrading the tetracycline antibiotics and restoration of environments polluted by the tetracycline antibiotics.

This invention belongs to the pharmaceutical field, specifically relating to a long-acting compound preparation of vonimuramin, its preparation method, and its application. The long-acting compound preparation of vonimuramin of this invention comprises the following components and their mass percentages: vonimuramin 5%–10%, doxycycline 10%–30%, tartaric acid 0.5%–2.0%, oil phase 10%–20%, emulsifier 10%–30%, co-emulsifier 2%–10%, and the balance being water. This invention, by preparing vonimuramin and doxycycline with corresponding excipients into a nanoemulsion, can effectively delay the release rate of vonimuramin, enabling the two drugs to be eliminated simultaneously in vivo, thus achieving a synergistic antibacterial effect. Furthermore, the drugs are encapsulated in the nanoemulsion core, which improves the solubility of vonimuramin in water while preventing doxycycline from forming insoluble complexes with metal ions in water, thereby improving the stability of the drugs.

This invention discloses a strain of *Pseudomonas nicosulfuron-methyl* capable of degrading multiple pollutants. Pseudomonas nicosulfuronedens HD1 and its applications belong to the field of functional microbial screening and application technology. This strain, originating from an aerobic composting environment of chicken manure (accession number GDMCC No:67351), can simultaneously and efficiently degrade nitropolycyclic aromatic hydrocarbons (NPH), polycyclic aromatic hydrocarbons (PAHs), and tetracycline antibiotics. In single pollutants at a concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceeded 97% after 8 hours; the degradation rate of nitrofluoranthene reached 99.01% after 48 hours; and the degradation rate of phenanthrene reached 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the degradation rate remained above 78% after 48 hours. This strain exhibits high degradation efficiency and strong environmental adaptability, providing a new microbial resource for the bioremediation of multi-contaminated sites and possessing broad application prospects.

Disclosed are a method for screening a riboswitch capable of specifically recognizing doxycycline as well as rational engineering and application of the riboswitch, and belongs to the field of gene expression regulation. The present disclosure provides a doxycycline specific riboswitch, and establishes a high-throughput screening method based on flow cytometry, and obtains the doxycycline riboswitch with further improved activation fold by way of computer-aided prediction of binding sites of the riboswitch and calculation of mutation sites. According to a dose-response curve, the riboswitch shows linear correlation at 40-100 μg / L, and meanwhile, a whole-cell sensor constructed is low in price, high in stability and simple to operate, and can be used for on-site rapid detection.

The invention discloses a p16-OSKM transgenic mouse model and a reprogramming senescence cell construction method and application, and belongs to the technical field of cell biology and regenerative medicine. According to the method, a p16-OSKM hybrid mouse capable of specifically inducing an OSKM factor in a p16 high expression cell is obtained on the basis of hybridization of a p16-Ink4a-CreERT2 transgenic mouse, a Rosa26-CAG-LSL-rtTA3-IRES-mKate2 transgenic mouse and a Col1a1-TRE-OSKM-IRES-mCherry transgenic mouse. The selective activation of the OSKM factor in senescent cells with high expression of p16 can be realized by adding doxycycline into primary embryo fibroblasts separated from the mouse under an in-vitro culture condition, so that the senescent cells enter a cell cycle again and recover division and multiplication capacities. The technology provides an accurate model platform for basic research, and can be used for aging mechanism exploration, cell fate regulation and control, chronic disease treatment, development and screening of anti-aging drugs and the like.

The invention discloses a method for efficiently constructing a personalized pancreatic cancer organoid and screening a tumor reactive TCR (T cell receptor), which comprises the following steps: transferring RSPO1 and WNT3A genes into the pancreatic cancer organoid, and inducing expression and secretion of RSPO1 and WNT3A in the organoid by adding Dox (doxycycline). The pancreatic cancer antigen tumor reactive TCR can be screened from the PBMC of a pancreatic cancer patient by using the constructed transgenic organ.

The invention provides a microbial composition for degrading doxycycline. The microbial composition is prepared from the following strains: acinetobacter Guilin HZJK-2, achromobacter sp. Adx-3 and plant growth-promoting bacteria JX15, wherein the acinetobacter Guilin HZJK-2 and the achromobacter sp. Adx-3 are used for degrading doxycycline; wherein the preservation number of the acinetobacter Guilin HZJK-2 is CGMCC (China General Microbiological Culture Collection Center) No.22143; the preservation number of the achromobacter adx-3 is CCTCC (China Center for Type Culture Collection) NO: M 208176; the preservation number of the plant growth-promoting bacterium JX15 is CGMCC (China General Microbiological Culture Collection Center) No.5622. The microbial composition provided by the invention has an excellent degradation effect on doxycycline, and the degradation rate exceeds 93%.

The application belongs to the field of materials and water treatment, and provides a preparation of lanthanum-containing schroeckingerite material and application of the material in antibiotic wastewater treatment, comprising: adding schroeckingerite to an alkali solution, then adding a La salt, performing a reaction, aging, collecting a precipitate, washing, drying, grinding, and obtaining the lanthanum-containing schroeckingerite material; the mass ratio of the schroeckingerite to the La salt is (1-2):(0.147-1.47). The lanthanum-containing schroeckingerite material can quickly remove antibiotic residues in water, has a simple preparation method, low cost, and a wide source of raw materials, and has a good application prospect. The material does not produce secondary pollution in the process of treating environmental pollutants, and is friendly to the environment. In the absence of external energy input, the lanthanum-containing schroeckingerite material can remove more than 90% of doxycycline, and is obviously superior to ordinary chemical synthesis of schroeckingerite.

The invention discloses a nano antibody Nb DX-6F capable of specifically recognizing doxycycline and an application of the nano antibody Nb DX-6F. The amino acid sequence of the VHH of the nano antibody Nb DX-6F is as shown in SEQ ID No.1, and the nano antibody has excellent organic solvent tolerance and acid and alkali tolerance. An ELISA detection method is established based on the nano antibody, the detection range of doxycycline by the method is 0.17-14.25 ng / mL, the half inhibitory concentration (IC50) is 1.34 ng / mL, the lowest limit of detection (LOD) is 0.05 ng / mL, and the detection sensitivity is high. Therefore, when the nano antibody Nb DX-6F is used for detecting doxycycline residues in an actual sample, the detection result is good in stability, high in sensitivity and high in accuracy, and the nano antibody has extremely high application value in detection of doxycycline or preparation of doxycycline detection products.

According to the application of the doxycycline and the compound composition of the doxycycline in preparation of the corneal injury repairing medicine, corneal collagenase is inhibited through the doxycycline, repairing of corneal epithelium is facilitated, and the oculentum preparation can act on cornea more enduringly, so that corneal repairing of alkali burn patients, dry eye patients and ocular surface injury patients is promoted, and the curative effect of the oculentum preparation is improved. According to the eye ointment, corneal epithelial cells can be effectively treated, regeneration and healing of the corneal epithelial cells are accelerated, hormones and non-steroidal anti-inflammatory components are added, ocular surface inflammatory response can be specifically inhibited, symptoms such as red and swollen eyes and pain can be relieved, the repairing effect of corneal epithelium can be enhanced, all the components are uniformly dispersed in the eye ointment by optimizing the preparation process, the stability of the eye ointment is guaranteed, and the eye ointment has good application prospects. The phenomena of layering, devitrification and the like are not easy to occur in the storage process, and stable and reliable product quality is ensured.

The present invention provides a single-plasmid inducible expression system and its preparation method and application, which belong to the field of genetic engineering technology. The single-plasmid inducible expression system comprises a single plasmid, and the nucleic acid sequence of the plasmid is shown in SEQ ID No. 1. The single-plasmid inducible expression system constructed by the present invention overcomes the complex operation of twice-establishing the system of traditional induced expression. Only one gene transduction is required to screen and obtain the expected transgenic cell line, which simplifies the operation process and improves efficiency. Puromycin can be used for clone screening after gene transduction, and the tetracycline antibiotic doxycycline is used to induce target gene expression. Compared with the lentiviral system, it is simple to operate, saves costs, can insert a larger exogenous gene length, and has a larger vector capacity; the background expression is lower, the induced expression multiple is higher, and efficient gene expression can be achieved while having lower background leakage.

The application provides a strain of Gilmaniella vishniacov Sarocladium kiliense ) 19-11C and an application thereof, preservation number of the Gilmaniella vishniacov Sarocladium kiliense ) 19-11C is CGMCC No.41846. As a fungus, the Gilmaniella vishniacov 19-11C is naturally insensitive to tetracycline antibiotics, can survive and play a degradation function in a high-concentration drug environment. The strain does not carry horizontally transferable bacterial drug resistance genes, fundamentally avoiding the potential environmental risk of causing drug resistance gene spread in the repair process. The strain shows wide adaptability and stability to changes in temperature, pH and nutritional conditions in the environment, effectively decomposes doxycycline through various biochemical pathways such as removal and oxidation, significantly reduces the biological toxicity, and provides an environmentally friendly and ecologically safe biological solution for the residue treatment of tetracycline antibiotics in the environment.

The invention relates to application of paeonol in preparation of a medicine for improving colorectal cancer tumor microenvironment and a colorectal cancer treatment medicine comprising paeonol and doxycycline, and belongs to the technical field of traditional Chinese medicine application. According to the paeonol, the tumor microenvironment is regulated by regulating tumor-related macrophages. When the paeonol is combined with the doxycycline to treat the colorectal cancer, the tumor microenvironment is regulated through the paeonol, so that the anti-colorectal cancer effect of the doxycycline is enhanced. The paeonol, the doxycycline and the combination of the paeonol and the doxycycline are used in colorectal cancer cell tests, tumor-related macrophage tests and mouse liver metastasis xenotransplantation model tests to verify that when the paeonol and the doxycycline are used together for treating colorectal cancer, the paeonol makes up the defect that the doxycycline is independently used; and the immunosuppressive tumor microenvironment cannot be effectively targeted, and a synergistic effect is formed by the immunosuppressive tumor microenvironment and the tumor microenvironment.