228 results about "Antimicrobial drug" patented technology

The invention discloses a full D-type antibacterial peptide with high enzymolysis stability and broad-spectrum antibacterial activity and application thereof. The antibacterial peptide is obtained by sequentially and repeatedly arranging D-type tryptophan, D-type arginine and D-type lysine for four times and carrying out C-terminal amidation; the amino acid sequence of the gene is (D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-NH2, and is marked as DWRK-12. The antibacterial peptide shows broad-spectrum antibacterial activity and excellent stability, can effectively resist clinically separated multidrug resistant strains, and keeps high cell selectivity at the same time. Due to the characteristics, the antibacterial peptide has important application value in the aspect of developing novel antibacterial drugs, especially in the field of treatment of infection of multiple drug-resistant bacteria.

The invention discloses acinetobacter baumannii and application of the acinetobacter baumannii in construction of a mastitis animal model. The acinetobacter baumannii strain is acinetobacter baumannii AbST3475NMG202308, and the preservation number of the acinetobacter baumannii strain is CGMCC (China General Microbiological Culture Collection Center) No.34450 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms). Experiments prove that the acinetobacter baumannii has high pathogenicity and can be used for establishing a typical animal model caused by acinetobacter baumannii infection, the animal model can be specifically a mastitis animal model, and a certain technical support is provided for subsequent research and development of drugs or vaccines; the acinetobacter baumannii can also be used for screening antibacterial drugs and developing diagnostic reagents and vaccines. The method has an important application value.

The invention belongs to the technical field of microorganisms, and particularly relates to application of marine phycobacterium LZ-14T in production of indole-3-acetic acid. According to the present invention, the interphycobacterium LZ-14T is named as Maricatenella alexandrii gen. Nov., sp. Nov. The present invention discovers that the interphycobacterium LZ-14T can achieve the effect of significantly inhibiting the synthesis of the PSP toxin by releasing the IAA signal molecule under the condition of promoting the growth of the host algae. The new discovery can provide a new theoretical basis for analyzing the generation mechanism of paralytic shellfish poison of alexandrium chainchain and exploring scientific prevention and control of red tide. The invention also finds that the IAA produced by the phycobacterium LZ-14T can inhibit the growth of escherichia coli, and the result lays a foundation for the preparation of new antibacterial drugs.

The application discloses a microbial drug sensitivity detection method. The application comprises: loading a microbial database, establishing a corresponding relationship between secondary microbial names and primary categories; loading a drug breakpoint database based on the "antimicrobial drug sensitivity experiment execution standard"; placing a standard culture dish in an image acquisition device to acquire an image, and calibrating an image analysis system with a vernier caliper; inputting sample information to be detected, acquiring and uploading the image; an image analysis system extracts the position of a drug sensitivity paper sheet, identifies the edge of a bacterial inhibition zone and calculates the diameter; the system extracts corresponding drug breakpoint thresholds based on the microbial category, compares the measured diameter and determines the sensitivity classification (S / I / R); and finally, the result is uploaded to a LIS system. The method can realize the automation, standardization and high efficiency of drug sensitivity detection.

Provided are a non-ribosomal antifungal peptide MaAFP1, a preparation method therefor and a use thereof. The molecular formula of the antifungal peptide MaAFP1 is C41H74N18O12, wherein an amino acid sequence from the N-terminus to the C-terminus is (D)Arg-(L)Arg(OH)-(D)Orn-(L)Thr-(D)Orn-(L)Arg(OH)-(D)Tyr. Fermentation is performed by using Metarhizium anisopliae CQMa421, a fermentation broth is collected, and separation and purification are performed by means of ion exchange chromatography and high performance liquid chromatography so as to obtain the antifungal peptide MaAFP1. The structure of MaAFP1 is analyzed by comprehensively using an Edman degradation method, mass spectrometry, infrared spectroscopy, nuclear magnetic resonance spectroscopy, and circular dichroism spectroscopy. By performing an in vitro bacteriostatic test, it is found that MaAFP1 has a good inhibitory effect on a variety of animal and plant pathogenic fungi, and has the value of the development of a novel antibacterial drug.

The invention belongs to the technical field of medicines, and particularly relates to an aryl dicarboxylic acid compound, a preparation method and application of the aryl dicarboxylic acid compound as an antibacterial agent. The compound is shown as a formula (1), wherein R1, R2, R3, R4, R5, R6, R7, R8, R9 and R10 are independently selected from hydrogen and halogen. Two ends of the molecule are connected through an N-phenylpropanamide connecting arm, the molecule is a compound with a novel structure, and the series of compounds have a relatively strong inhibition effect on acinetobacter baumannii and are worthy of further research and development.

The invention relates to the technical field of biological pharmacy, and particularly discloses application of antibacterial small molecule peptide in preparation of antibacterial infection drugs. The antibacterial small molecule peptide is VeAMP, and the amino acid sequence of the antibacterial small molecule peptide is MKIVKRILLVL-NH2. The small molecule peptide VeAMP provided by the invention shows remarkable antibacterial activity on pan-drug-resistant acinetobacter baumannii, has the advantages of rapid sterilization, low hemolysis, low cytotoxicity and good in-vivo safety, is not easy to induce bacterial drug resistance after being exposed for a long time at a sub-inhibitory concentration, can effectively reduce tissue bacterial load, remarkably improves the survival rate of infected mice, and has a good application prospect. The invention provides a novel and safe candidate antibacterial agent which is not easy to generate drug resistance for preventing and treating drug-resistant acinetobacter baumannii infection.

This invention discloses a polymer-loaded drug-eluting nanoparticle with high encapsulation efficiency and its application in coatings and surface film materials. The polymer-loaded drug-eluting nanoparticles are prepared via emulsion polymerization, simultaneously loading antibacterial drugs with different log P values. The preparation process is simple, and the resulting product has small particle size, high molecular weight, high uniformity, and high drug encapsulation efficiency, achieving the substitution of non-reactive surfactants with reactive surfactants. A coating can be prepared using the above-mentioned polymer-loaded drug-eluting nanoparticles. The coating can be further used to prepare sustained-release antibacterial surface film materials through cross-linking film formation, exhibiting good mechanical properties, excellent chemical resistance, and high safety. This invention can be widely applied to various environmental and material surfaces, including but not limited to surface modification of instruments and building materials in residential and medical environments, thereby achieving a long-lasting antibacterial effect.

This invention discloses a silk fibroin-based biomimetic artificial tympanic membrane with a gradient structure and its preparation method, comprising the following steps: encapsulating and dissolving an antibacterial drug in an organic solvent and then adding it dropwise to a silk fibroin solution; casting the film and inducing the formation of a β-sheet structure with methanol to obtain an outer dense layer; electrospinning the silk fibroin solution to obtain oriented silk fibroin fiber felt, and cross-linking it in an ethanol solution to obtain an intermediate fiber layer; mixing the silk fibroin solution with a pore-forming agent and casting it onto the intermediate fiber layer; covering the intermediate fiber layer with the outer dense layer; after pre-freezing and lyophilizing, immersing the membrane in deionized water to dissolve the pore-forming agent to form an inner porous layer; then placing it in a PBS buffer containing growth factors and shaking treatment; and finally drying to obtain the silk fibroin-based biomimetic artificial tympanic membrane with a gradient structure. This invention's three-layer biomimetic artificial tympanic membrane possesses antibacterial and regenerative functions, while also exhibiting excellent mechanical properties, meeting the requirements of a biomimetic tympanic membrane.

The application discloses a kind of β-hairpin structure self-assembly capture antibacterial peptide J-1-RF and its application, it is related to biological medicine technical field.The amino acid sequence of the β-hairpin structure self-assembly capture antibacterial peptide J-1-RF is as shown in SEQ ID NO.4.The application selects the broad-spectrum antibacterial short peptide J-1 derived from honeybee royal jelly as template peptide, designs and transforms according to the amino acid sequence characteristics of short peptide J-1, obtains hydrophilic and hydrophobic amino acid alternately arranged structure unit, and uses it as self-assembly peptide fiber skeleton. D PG and RRRF two turning sequences are used for β-hairpin peptide design, so that it has the dual effect of antibacterial and bacterial capture.The β-hairpin structure self-assembly capture antibacterial peptide J-1-RF provided by the application can form fiber network structure, has good bacteriostatic effect and high stability, and has excellent biocompatibility, which provides effective technical support for developing new antibacterial drugs.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

This invention provides the application of Vibrio receptor protein type VI collagen α2 from the tongue sole as a target protein for blocking Vibrio pathogen adhesion and infection of tongue sole cells, providing an important foundation for the research and development of strategies and disease control technologies for the prevention and treatment of vibrio infections in tongue sole. This invention discovers that tongue sole type VI collagen α2 can mediate the binding of various Vibrio pathogens to tongue sole, and can serve as a candidate protein for blocking Vibrio adhesion and infection of tongue sole cells, thus providing a new strategy for the prevention and treatment of vibrio diseases in tongue sole. This invention also provides a new approach for the development of novel antibacterial drugs, which has application value in the prevention and treatment of tongue sole diseases.

A method of coating a metallic substrate includes immersing a metallic substrate in a polymer composition including a conductive 3,4-ethylene dioxythiophene (EDOT) polymer, a gelatin polyelectrolyte, an antibacterial drug, an organic solvent, and an inorganic salt solute, and further coating the polymer composition onto a surface of the metallic substrate by cyclic voltammetry to form a coating on the surface of the metallic substrate. The coating on the metallic substrate is performed such that the gelatin and the antibacterial drug are uniformly distributed throughout the coating, and the coating has a thickness between 7.0 and 15.0 micrometers. The metallic substrate is a stainless steel (SS). An implantable medical device including a metallic substrate coated by the present method.

The invention discloses a perfluoroalkyl-substituted pyrimido [1, 2-b] indazole ketone compound and a preparation method thereof, and belongs to the technical field of compounds containing two or more heterocyclic rings. Comprising the following steps: with enaminone, a perfluoroalkane compound and aminoindazole as raw materials, carrying out a reaction under blue light irradiation in the presence of an organic solvent and alkali to prepare the perfluoroalkyl-substituted pyrimido [1, 2-b] indazole ketone compound; according to the invention, through visible light mediated dehalogenation and cyclization free radical addition, high-yield synthesis of the perfluoroalkyl substituted pyrimido [1, 2-b] indazole ketone compound is realized; the prepared perfluoroalkyl-substituted pyrimido [1, 2-b] indazolone compound has excellent antibacterial activity, can be used for preparing antibacterial drugs, and has outstanding industrial application value.

This invention belongs to the field of antimicrobial peptide technology, and particularly relates to a broad-spectrum antimicrobial peptide with low hemolytic activity and its applications, as well as a broad-spectrum antibacterial drug. The antimicrobial peptide is named AY6, and its amino acid sequence is shown in SEQ ID NO.1. Antimicrobial peptide AY6 exhibits significant antibacterial effects against both Gram-positive and Gram-negative bacteria, with a bactericidal rate far exceeding that of traditional antibiotics, enabling highly effective responses to bacterial infections. AY6 has low hemolytic activity and good biocompatibility, making it suitable for medical anti-infective applications. The antimicrobial peptide provided in this application has a short sequence, is easy to synthesize chemically, can save on large-scale production costs, and has broad application prospects.

The invention relates to the technical field of chemical synthesis of medicines, in particular to a pleuromutilin derivative, a preparation method thereof and an antibacterial active composition. The structural formula of the pleuromutilin derivative is a compound with a structure as shown in a formula I, or pharmaceutically and / or veterinarily acceptable salt, a solvent compound, an optical isomer and a polymorphic compound of the pleuromutilin derivative, wherein R is selected from any one of a group consisting of R and R. According to the scheme provided by the invention, the compound has good antibacterial activity and is suitable for being used as a novel antibacterial drug for preventing and treating human or animal bacterial infectious diseases.

This invention belongs to the field of pharmaceutical technology, specifically relating to biomimetic nanomedicines for treating subcutaneous drug-resistant bacterial infections, their preparation methods, and applications. In this invention, dopamine hydrochloride is added during the synthesis of a metal-organic framework to prepare a dandelion-shaped carbon nanozyme precursor. This precursor is then carbonized to obtain a carbon nanozyme with targeting, photoresponsiveness, and peroxidase activity. Finally, berberine hydrochloride is loaded onto the carbon nanozyme to obtain the biomimetic nanomedicine. The biomimetic nanomedicine provided by this invention firstly possesses good biocompatibility; secondly, it has a unique dandelion-like morphology, which is beneficial for its targeting effect; and finally, it exhibits strong photoresponsiveness in the near-infrared region, enabling synergistic and efficient bactericidal action through the mechanisms of antibacterial drugs, photothermal therapy, and chemokinetics. It has significant application value in the preparation of drugs for treating subcutaneous drug-resistant bacterial infections.

The invention provides a compound for preparing an MBL and / or SBL enzyme inhibitor, application of the compound and a medicine of the compound, and belongs to the field of medicine. The structure of the compound is shown as a formula I. The compound provided by the invention has good and broad-spectrum inhibitory activity on clinically common metal beta lactamase (MBL) and serine beta lactamase (SBL), can be used as an MBL and / or SBL enzyme inhibitor, and is used for preparing antibacterial drugs, especially drugs for resisting drug-resistant bacteria. In addition, the compound disclosed by the invention is combined with beta-lactam antibiotic meropenem and the like for use, so that the compound has relatively good antibacterial activity on various beta-lactam antibiotic drug-resistant bacteria. The compound disclosed by the invention has great potential in preparation of MBL and SBL dual broad-spectrum inhibitors and medicines for resisting beta-lactam antibiotic-resistant bacteria. The invention provides a new choice for the use of antibacterial drugs, and has a good application prospect.

The invention relates to the technical field of gels, and particularly discloses a wound repair gel responding to a wound surface microenvironment and a preparation method thereof, the gel is prepared from the following raw materials in percentage by mass: 3.0%-6.0% of a response gel matrix, 0.5%-2.0% of an active pharmaceutical ingredient, 0.5%-1.2% of a bionic repair component, 2.0%-4.0% of a moisturizing and stabilizing agent and the balance of water for injection. The response gel matrix comprises oxidized sodium alginate and methylacryloylated chitosan, the active drug component comprises a pH sensitive antibacterial drug and an enzyme sensitive healing promoting drug, and the bionic repairing component is a compound of recombinant human collagen and zinc sulfate. Bacterial reproduction is timely inhibited and infection is controlled at the early stage of wound healing; after entering the later healing stage, the composition continuously promotes epithelial cell proliferation and tissue regeneration, and meanwhile, the composition can remarkably promote tissue regeneration, reduce scar formation and realize antibacterial and healing-promoting synergy.

The invention discloses a self-assembled nano-capture antibacterial peptide KIL4 and a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence is shown as SEQ ID No.1. The preparation method comprises the following steps: adopting an alpha-spiral heptapeptide repetitive sequence (abcdefg) 4 template, and selecting lysine to provide positive charges at positions b and c; isoleucine and leucine are respectively filled to a position a and a position d to provide intermolecular hydrophobic force, and lysine and glutamic acid are selected to be respectively filled to a position e and a position g to provide intermolecular electrostatic force; the position f of the center of the hydrophilic surface of the polypeptide is filled with tryptophan to optimize the hydrophobicity of the polypeptide, on the basis, the sequence is repeated for four times, and the invention further discloses application of the polypeptide in preparation of drugs for treating gram-positive bacteria or / and gram-negative bacteria infectious diseases. The antibacterial peptide disclosed by the invention has dual effects of resisting bacteria and capturing bacteria, has excellent biocompatibility, and provides an effective technical support for developing novel antibacterial drugs.

The application discloses application of antibacterial peptide BMAP-28 and antibiotics in combined preparation of antibacterial drugs and belongs to the technical field of biological medicine. The antibiotics are selected from one or more of the following: ciprofloxacin, gentamicin and cefixime. The application combines the antibacterial peptide BMAP-28 with antibiotics such as ciprofloxacin and gentamicin, finds that the combination has a significant synergistic antibacterial effect on Enterobacter sakazii, can greatly save the dosage of the drug, reduce the toxic side effect and block the evolution process of drug resistance. Through the destruction of the bacterial cell membrane and the assistance of the antibiotic effect, the combination can effectively overcome the multiple drug resistance, widen the antibacterial spectrum and has a wide clinical application prospect.

The application belongs to the technical field of pharmaceutical chemistry, and particularly relates to a pyrimidine and thienopyrimidine derivative, a preparation method and application thereof. The pyrimidine derivative in the application is a compound with the structure of formula I, and the thienopyrimidine derivative is a compound with the structure of formula II. The pyrimidine and thienopyrimidine derivative can be used as an AcrB efflux pump inhibitor, and can significantly enhance the antibacterial activity when combined with an antibacterial drug. The Nile red experiment shows that the compound 64c can completely inhibit the AcrB-mediated efflux at a concentration of 50 μM, and the effect is better than that of the positive control PA beta N. The compound in the application has novel structure and excellent activity, and can be used for preparing an AcrB efflux pump inhibitor for reversing bacterial drug resistance and an antibacterial sensitizing drug.

A method of treating a wound may include topically administering a statin composition including a statin selected from atorvastatin, fluvastatin, lovastatin, pitavastatin, pravastatin, rosuvastatin, simvastatin, or combination thereof. The statin composition may be administered in an ointment, powder, or liquid format. The method may also include topically administering one or more antimicrobial drugs.

A system and method for identification of target sites in a pathogenic genome and combating a pathogenic infection have been provided. The present disclosure utilizes the fact that a conserved stretch of nucleotide sequence in genomic neighborhood of genes important for bacteria can be targeted to disrupt the overall functioning of the pathogen. The method involves identification of nucleotide repeat sequences in the DNA. The method and system also involve administration of a cocktail comprising antimicrobial drugs, biofilm inhibitors and a construct. The genomic neighborhood or vicinity or ‘flanking genes’ refers to regions lying within a predefined number of genes to the identified conserved stretch of nucleotide repeat sequence (or its reverse complement) on the candidate pathogen genome or within a distance of predefined number of bases with respect to the conserved stretch of nucleotide repeat sequence (or its reverse complement) on the candidate pathogen genome.

The invention discloses a silk fibroin-based bionic artificial tympanic membrane with a gradient structure and a preparation method thereof, and the preparation method comprises the following steps: wrapping and dissolving an antibacterial drug in an organic solvent, then dropwise adding into a silk fibroin solution, casting to form a membrane, and then inducing by methanol to form a beta-folded structure to obtain an outer compact layer; carrying out electrostatic spinning on the silk fibroin solution to obtain a directionally arranged silk fibroin fiber felt, and soaking and cross-linking in an ethanol solution to obtain a middle fiber layer; mixing the silk fibroin solution with a pore-foaming agent, and pouring the mixture on the middle fiber layer; covering the middle fiber layer with the outer compact layer, pre-freezing and freeze-drying, immersing into deionized water to dissolve out the pore-foaming agent so as to form an inner porous layer, putting into a PBS buffer solution containing growth factors for oscillation treatment, and drying, thereby obtaining the silk fibroin-based bionic artificial tympanic membrane with the gradient structure. The artificial tympanic membrane with the three-layer bionic structure has antibacterial and regeneration promoting functions, has excellent mechanical properties and meets the requirements of bionic tympanic membranes.

This invention discloses an antimicrobial peptide SI-20 and its application in the preparation of antimicrobial drugs, belonging to the field of biomedical technology. The amino acid sequence of the antimicrobial peptide SI-20 is shown in SEQ ID NO.2. The antimicrobial peptide SI-20 provided by this invention has a highly efficient killing ability against a variety of Gram-negative / positive bacteria, effectively retaining antimicrobial activity while significantly reducing cytotoxicity and improving therapeutic efficacy. Simultaneously, this antimicrobial peptide SI-20 exhibits good stability in serum environments and under heat treatment, strongly inhibits biofilm formation, and possesses rapid and efficient antimicrobial properties, providing a highly promising candidate resource for the development of highly effective and low-toxicity antimicrobial drugs, with broad application potential.

The present application relates to the new use of Baotouweng decoction in the synergistic antibacterial effect of ceftriaxone, tetracycline and amikacin antibiotics. In the preparation of the composition or compound preparation, the dosage ratio of Baotouweng decoction and ceftriaxone, tetracycline and amikacin is between 6400:1-12800:1. The FIC index of Baotouweng decoction combined with ceftriaxone, amikacin or tetracycline is 0.1875-0.375, which is less than 0.5, that is, Baotouweng decoction combined with ceftriaxone, amikacin or tetracycline has synergistic antibacterial effect. The present application can effectively reduce the dosage of ceftriaxone, amikacin or tetracycline, and the MIC value of Baotouweng decoction combined with ceftriaxone, amikacin or tetracycline is reduced by 2-16 times compared with that of single use. Baotouweng decoction can be used as an antibacterial drug resistance reversal agent to improve the antibacterial therapeutic effect under the condition of reducing the dosage of antibacterial drugs.

The invention belongs to the technical field of biology, and discloses a polypeptide derived from a deep sea ecological environment, an application of the polypeptide in preparation of an antibacterial preparation and an antibacterial drug. According to the application of the polypeptide disclosed by the invention in the preparation of the antibacterial preparation, the polypeptide comprises one or more of amino acid fragments with sequences shown as SEQ ID NO: 1-9, the compound has good bacteriostatic or bactericidal activity on one or more of escherichia coli, halomonas aeruginosa, acinetobacter picoteri, staphylococcus aureus, linear preristeria, klebsiella pneumoniae and vibrio parahaemolyticus, and has extremely low toxicity on mammalian cells under an effective concentration. The compound has excellent bacteriostatic activity, bacteriostatic broad-spectrum property and biocompatibility, and has very excellent application prospects in multiple fields such as food preservation, agricultural disease prevention and control, feed additives and the like.

The invention discloses an antibacterial peptide and application thereof in preventing and treating dental plaque and gingival problems, and belongs to the technical field of biology. According to the invention, two cationic antibacterial peptides LR18 and LR18-2 with amphiphilic alpha-helical structures are designed from the beginning. The two antibacterial peptides have good antibacterial and bactericidal activity on oral periodontal pathogenic bacteria (including fusobacterium nucleatum, porphyromonas gingivalis and streptococcus mutans), the minimum inhibitory concentration (MIC) is 62.5-500 [mu] g / mL, and the minimum bactericidal concentration (MBC) is 62.5-2000 [mu] g / mL. The structure is simple, the chemical synthesis difficulty is low, a high-purity product can be directly synthesized, the cell hemolytic activity is low, the safety is high, the product can be used for preparing oral antibacterial drugs and / or oral care products, and oral diseases are prevented and treated by inhibiting the growth of oral periodontal pathogenic bacteria.