653 results about "Antimicrobial drug" patented technology

The invention discloses application of GGTI298 in prevention and control of rice blast, and belongs to the technical field of rice blast prevention and control. According to the technical scheme, the novel medical application of the GGTI298 is disclosed, and the GGTI298 has good antibacterial activity on magnaporthe oryzae and can inhibit conidia germination and appressorium formation of the magnaporthe oryzae so as to influence pathogenicity. The GGTI298 has a prospect of preventing and treating magnaporthe oryzae infection, and a reference basis is provided for development of antibacterial drugs based on a GGTI298 pilot structure.

The invention discloses application of a low-toxicity broad-spectrum antibacterial peptide taking WW as a symmetric center, WK is repeated twice and symmetrically distributed on two sides, WW is taken as the symmetric center to obtain an antibacterial peptide WKWKWWKWKW-NH2 (N2W2), and tryptophan at the C terminal and the N terminal of the N2W2 is replaced with leucine and / or isoleucine to obtain the antibacterial peptide. The antibacterial peptide can basically maintain the excellent antibacterial activity of the parent peptide, and the hemolytic activity of the antibacterial peptide is greatly reduced, so that the safety is improved. Wherein the antibacterial activity of IL-N2W2 is the highest, but the stability of IL-N2W2 needs to be improved, so that D-IL-N2W2 is obtained by carrying out D-type amino acid substitution on IL-N2W2. The D-IL-N2W2 keeps the high antibacterial activity and low toxicity of IL-N2W2, can still completely inhibit the growth of bacteria after being incubated with high-concentration trypsin or chymotrypsin, has great potential in the aspect of relieving the drug resistance of antibiotics, and has good application prospects in preparation of clinical antibacterial drugs.

The invention discloses a full D-type antibacterial peptide with high enzymolysis stability and broad-spectrum antibacterial activity and application thereof. The antibacterial peptide is obtained by sequentially and repeatedly arranging D-type tryptophan, D-type arginine and D-type lysine for four times and carrying out C-terminal amidation; the amino acid sequence of the gene is (D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-NH2, and is marked as DWRK-12. The antibacterial peptide shows broad-spectrum antibacterial activity and excellent stability, can effectively resist clinically separated multidrug resistant strains, and keeps high cell selectivity at the same time. Due to the characteristics, the antibacterial peptide has important application value in the aspect of developing novel antibacterial drugs, especially in the field of treatment of infection of multiple drug-resistant bacteria.

The invention discloses a preparation method of an antibacterial PEEK material for bone implantation. The preparation method comprises the following steps: carrying out sand blasting treatment on the surface of a PEEK sample; immersing the sandblasted PEEK sample into a mixed solution containing concentrated sulfuric acid and hydrogen peroxide, and carrying out chemical sulfonation treatment; immersing the sulfonated PEEK sample into a dopamine solution, so that dopamine is oxidized and self-polymerized on the surface to form a PDA coating; immersing the PEEK sample with the PDA coating into an antibacterial drug solution, so that the antibacterial drug is loaded on the surface; carrying out heat treatment on the PEEK sample loaded with the antibacterial drug; thoroughly cleaning the sample, removing uncombined antibacterial drug molecules, and drying; through sand blasting treatment and sulfonation treatment, the surface roughness and hydrophilicity of the PEEK material are remarkably improved, and favorable conditions are provided for adhesion of a dopamine coating and an antibacterial agent; due to introduction of the dopamine coating, the biocompatibility and the adhesion of the material are enhanced, and the dopamine coating also serves as an effective carrier of antibacterial drugs, so that the antibacterial function is maximized.

The invention relates to a preparation method of antibacterial gamma-polyglutamic acid superabsorbent hydrogel. The preparation method comprises the following steps: (1) preparing a gamma-polyglutamic acid solution; (2) dissolving an antibacterial agent in the gamma-polyglutamic acid solution, and fully stirring and uniformly mixing to obtain a mixed solution 1; adjusting the pH value of the mixed solution 1, heating the mixed solution 1 to 60 DEG C, and continuously reacting for 1-2 hours to obtain a mixed solution 2; cooling the mixed solution 2 to room temperature, adding a cross-linking agent, and uniformly mixing to obtain a mixed solution 3; standing the mixed solution 3 at room temperature for 1-3 hours and then carrying out gradient heating reaction to obtain an antibacterial gelatinous product; and (3) dehydrating the antibacterial gelatinous product, and carrying out vacuum negative pressure drying to obtain a dried hydrogel product, namely the antibacterial gamma-polyglutamic acid super absorbent hydrogel. The antibacterial gamma-polyglutamic acid superabsorbent hydrogel prepared by the invention has good biocompatibility, is safe and non-toxic, also has excellent water absorption performance and antibacterial performance, and has a good application prospect.

The invention provides a patient data management system based on a cloud platform, and relates to the field of intelligent management. And then performing semantic analysis and associated coding fusion on the basic information and the operation information of the patient by adopting a data analysis technology based on a natural language to obtain an antibacterial drug use reference information fusion semantic feature vector of the patient, and generating an antibacterial drug name suitable for the condition of the patient based on the vector obtained by fusion. In this way, the defects of manual medicine selection according to experience can be overcome to a certain extent, and then the accuracy and rationality of use of the antibacterial medicine are effectively improved.

The invention provides a plant lactobacillus YH001 with bacteriostatic activity as well as a screening method and application of the plant lactobacillus YH001. The phytobacterium plantarum YH001 has the taxonomic name of Lactiplantibacillus plantarum, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No: 65693, the preservation number of Lactiplantibacillus plantarum YH001 is CGMCC NO: 65693, and the preservation number is CGMCC NO: 65693. The preservation date is December 30, 2024. The strain has excellent biological characteristics of no hemolysis, acid resistance, cholate resistance and the like, and has wide application potential in the fields of food, medicine and the like. The fermentation supernatant still keeps high bacteriostatic activity after being treated at a high temperature of 121 DEG C, and shows excellent thermal stability. Therefore, the strain has a wide application prospect in research and development of antibacterial agents, food leavening agents and preservative products.

The invention discloses an antibacterial drug delivery system based on a dynamic response type microneedle patch and a preparation method, and belongs to the field of biomedical materials.The antibacterial drug delivery system comprises a substrate layer and a needle point layer arranged on the surface of the substrate layer, the substrate layer is a mixed matrix of polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), and the needle point layer is made of nano-silver; the needle tip layer is a phenylboronic acid modified oxidized hyaluronic acid and polyvinyl alcohol composite system. The antibacterial drug delivery system based on the dynamic response type microneedle patch, namely the microneedle patch with the pH response drug release function, is suitable for antibacterial treatment of infectious wounds, the mechanical strength of the needle point layer is improved, it can be better guaranteed that a microneedle completely penetrates into the skin, and the delivery efficiency is improved; meanwhile, drug release can be responded according to the pH of a bacterial infection part, and the antibacterial effect is good.

The invention discloses a beta-hairpin structure self-assembly capture antibacterial peptide and application thereof, and relates to the technical field of biological medicine. The amino acid sequence of the beta-hairpin structure self-assembly capture antibacterial peptide is as shown in any one of SEQ ID NO. 3-6. A broad-spectrum antibacterial short peptide Jelleine-1 (J-1) from bee royal jelly is selected as a template peptide, design and transformation are performed according to amino acid sequence characteristics of the short peptide J-1, and a hydrophilic and hydrophobic amino acid alternate arrangement structural unit is obtained and serves as a self-assembly peptide fiber skeleton. Beta-hairpin peptide design is carried out by adopting two turning sequences of DPG and RRRF, so that the beta-hairpin peptide has dual effects of resisting bacteria and capturing bacteria. The beta-hairpin structure self-assembly capture antibacterial peptide provided by the invention can form a fiber network structure, has a good antibacterial effect, high stability and excellent biocompatibility, and provides an effective technical support for developing novel antibacterial drugs.

The invention discloses an ultrashort-sequence antibacterial peptide rich in lysine and tryptophan, an antibacterial peptide hydrogel and application of the ultrashort-sequence antibacterial peptide and the antibacterial peptide hydrogel. The preparation method comprises the following steps: combining lysine and tryptophan to obtain polypeptide containing five or six amino acids; then amidation is carried out on the C terminal of the polypeptide, fatty acid chain modification is carried out on the N terminal and side chain amino groups, the ultra-short sequence antibacterial peptide rich in lysine and tryptophan is obtained, and the antibacterial peptide has broad-spectrum antibacterial activity for resisting gram-positive and gram-negative bacteria. An antibacterial peptide micelle self-assembly experiment shows that C12-2KW can be self-assembled into stable hydrogel in a 7.17 mu M PBS (Phosphate Buffer Solution), the hydrogel internally has a compact net-shaped structure, can completely cover the peritoneal injury surface and physically isolate the peritoneal injury surface from surrounding tissues, and has a good effect on treating wound infection caused by MRSA (Methicillin-Resistant Staphylococcus Aureus); meanwhile, the compound has the effect of resisting intestinal wall and abdominal wall adhesion after an operation, is low in toxicity, has a very good application prospect in the aspect of preparing clinical antibacterial drugs, and is expected to become a candidate drug of a novel antibiotic.

The invention belongs to the technical field of medicines, and particularly relates to a bionic nano-drug for resisting subcutaneous drug-resistant bacterial infection as well as a preparation method and application thereof. Dopamine hydrochloride is added in the synthesis process of a metal-organic framework to prepare a dandelion-shaped carbon nano-enzyme precursor, then the nano-enzyme precursor is carbonized to obtain a carbon nano-enzyme with targeting property, photoresponse and peroxidase activity, and finally berberine hydrochloride is loaded on the carbon nano-enzyme to obtain the bionic nano-drug. The bionic nano-drug provided by the invention firstly has good biocompatibility, secondly has a unique dandelion-shaped form, is beneficial to playing a targeting role, and finally has relatively strong light response capability in a near-infrared region, and can synergistically and efficiently sterilize through action mechanisms of antibacterial drugs, photothermal therapy and chemical kinetics, so that the bionic nano-drug has a good application prospect. The compound has an important application value in preparation of drugs for treating subcutaneous drug-resistant bacterium infection.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention discloses a preparation method of ocimum gratissimum carbon dots and application of the ocimum gratissimum carbon dots in antibacterial drugs and food preservation. The preparation method of the ocimum gratissimum carbon dots comprises the following steps: performing steam distillation on ocimum gratissimum leaves to remove essential oil, drying in the shade, and crushing; adding an alcohol solvent into the powder, extracting, filtering, concentrating and drying to obtain an alcohol extract; dispersing the alcohol extract in distilled water, and stirring and pyrolyzing on a heating table; and diluting the pyrolysis product with distilled water, centrifuging, taking supernate, filtering with a filter membrane, dialyzing, and freeze-drying to obtain the carbon dots. The obtained carbon dots have a carbon dot-vesicle coexisting structure, and the average particle size is 5.732 + / -0.5 nm. The prepared ocimum gratissimum carbon dots can be applied to preparation of antibacterial drugs, especially drugs for inhibiting the activity of bacillus cereus and listeria, and the field of food preservation, and have the advantages of being wide in raw material source, simple in preparation method, low in cost, environmentally friendly and the like.

The invention discloses a copper ion complex based on a protein template as well as a preparation method and application of the copper ion complex. The copper ion complex takes a protein template as a carrier, and is obtained by in-situ complexing Cu < 2 + > with a copper ion carrier; the preparation method is a one-step green synthesis technology, mild synthesis conditions not only avoid the use of an organic solvent, but also do not need large-scale instruments and the like, so that the synthesis cost is greatly saved, and the formed copper ion complex has the advantages of high drug effect, small toxic and side effects, lasting effect, good stability and the like, and is suitable for industrial production. The problems that an existing copper ion complex is poor in solubility and low in bioavailability are solved, and the copper ion complex is applied to preparation of anti-tumor and / or antibacterial drugs and has extremely high anti-tumor and antibacterial activity.

The invention discloses a nano-drug delivery method for enhancing LGP kidney targeted release and application of the nano-drug delivery method, and relates to the technical field of nano-drugs. According to the nano-drug delivery method for enhancing LGP kidney targeted release and the application thereof, a hydrophobic modified chitosan polymer is synthesized; preparing HCS nanoparticles; and constructing a drug loaded nanoparticle, namely, HCS-LGP NPs. According to the invention, the solubility of the medicine is increased, the distribution of the medicine in vivo is changed, and the targeting of the medicine is improved, so that the treatment effect is improved, and adverse reactions are reduced. Due to good biocompatibility and modifiability, chitosan is widely used as a nano delivery carrier of anti-tumor and antibacterial drugs.

The invention relates to plant lactobacillus and application thereof. Wherein the gene of the molecular marker for the plant lactobacillus has a nucleotide sequence selected from one of the following sequences: (1) a nucleotide sequence as shown in SEQ ID NO: 24; (2) a nucleotide sequence having at least 97%, 98%, 99% or higher homology with the nucleotide sequence as shown in SEQ ID NO: 24; and (3) a nucleotide sequence with one or more nucleotide sequences, such as 1, 2, 3, 4, 5 or more nucleotide substitutions, deletion or insertion, in the nucleotide sequence as shown in SEQ ID NO: 24. The plant lactobacillus provided by the invention is non-toxic, has good biological characteristics, has a good curative effect on prevention and / or treatment of vaginal infection and related diseases, and avoids the problems of increased drug resistance, high recurrence rate and the like caused by use of antibiotic and other antibacterial drugs during treatment of vaginal infection and related diseases.

The invention belongs to the field of polypeptides, and particularly relates to an antibacterial peptide derived from marine microorganisms, an antibacterial composition and application. Based on a deep learning algorithm, five potential antibacterial peptides are mined from a global marine microbiome database, and the five antibacterial peptides are expressed in yarrowia lipolytica. Experimental tests show that the five antibacterial peptides have differentiated antibacterial effects on different bacteria, and the antibacterial peptide shown in SEQ ID NO: 3 can have an obvious inhibition effect on escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, has low hemolysis and has a positive application prospect. The antibacterial peptide disclosed by the invention has the potential of further developing novel antibacterial drugs or antibacterial additives.

The invention discloses an antibacterial peptide as well as a preparation method and application thereof. The antibacterial peptide has an amino acid sequence as shown in any one of SEQ ID NO: 1-11. The antibacterial peptide prepared by the scheme provided by the invention has very strong bacteriostasis and sterilization capabilities on methicillin-resistant staphylococcus aureus and multidrug-resistant escherichia coli, has application potential of becoming an antibacterial agent, a preservative or a disinfectant, and is not easy to generate drug resistance.

The invention discloses an antibacterial peptide PPI45 / PPI47 as well as a preparation method and application of the antibacterial peptide PPI45 / PPI47. According to the invention, fungal defensin Plectasin is used as a template, and the self-assembled antibacterial peptide PPI45 / PPI47 is designed. The expression of the self-assembled antibacterial peptide PPI45 / PPI47 in pichia pastoris is realized by optimizing the gene sequence of the antibacterial peptide and constructing a specific expression vector, a perfect purification system is established, large-scale production can be realized, and the self-assembled antibacterial peptide PPI45 / PPI47 can be applied to the fields of antibacterial drug development, hydrogel wound treatment and the like, and has wide application value and market prospect.

The invention provides a marine biological alkali compound. The chemical formula of the marine biological alkali compound is as follows: # imgabs0. The marine biological alkali compound or pharmaceutically acceptable salt thereof can be used for preparing antibacterial drugs or anti-infective drugs. The invention also provides a separation and extraction preparation method or a chemical synthesis preparation method of the marine alkaloid compound or the pharmaceutically acceptable salt thereof, and the preparation method is simple and easy to industrialize. The invention also provides application of the marine biological alkali compound or the pharmaceutically acceptable salt thereof in preparation of antibacterial drugs or anti-infection drugs for human or fish.

The invention discloses a phenanthro [9, 10-d] imidazole quaternary ammonium salt derivative as well as preparation and antibacterial application thereof. According to the series of compounds, phenanthro [9, 10-d] imidazole quaternary ammonium salt derivatives which are novel in structure and good in antibacterial activity are prepared by taking phenanthro quinone as a lead compound and modifying the structure of phenanthro quinone, the structural general formula of the phenanthro [9, 10-d] imidazole quaternary ammonium salt derivatives is shown in the specification, and R is selected from H, C6-C14 alkyl or C3-C8 alkylene. The phenanthro [9, 10-d] imidazole quaternary ammonium salt derivative prepared by the invention has a relatively good antibacterial effect on gram-positive bacteria such as staphylococcus aureus S.aureus ATCC 29213 and various clinical methicillin-resistant staphylococcus aureus (MRSA), meanwhile, the water solubility is improved, the biotoxicity of a parent body is reduced, and the phenanthro [9, 10-d] imidazole quaternary ammonium salt derivative has relatively high yield and is suitable for industrial production. The compound is expected to be further developed into a clinical candidate antibacterial drug. # imgabs0 #

The invention discloses a klebsiella pneumoniae bacteriophage vBKpnPGZMUVR2301 and application thereof, and relates to the technical field of biology. The klebsiella pneumoniae bacteriophage vBKpnPGZMUVR2301 is preserved in Guangdong Microbial Culture Collection Center on October 14, 2024, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 65268-B1. The bacteriophage keeps stable titer in a wide pH range (4-10) and a temperature interval (4-50 DEG C), and the highest titer can reach more than 109 pfu / mL. The bacteriophage can efficiently split klebsiella pneumoniae. The invention provides powerful technical support for developing novel antibacterial drugs and treatment schemes for klebsiella pneumoniae, and shows wide application prospect and potential clinical value.

The invention belongs to the field of antibacterial peptides, and particularly relates to a marine antibacterial peptide based on machine learning mining and application thereof. The invention discloses a method for mining marine antibacterial peptides based on machine learning, and 10 marine antibacterial peptides are screened, and the amino acid sequence of the marine antibacterial peptides is shown as any one of SEQ ID NO: 1-10. Wherein the Peptide 5 and the Peptide 8 have a broad-spectrum antibacterial characteristic, and the drug-resistant bacteria MIC (Minimum Inhibitory Concentration) of the Peptide 5 and the Peptide 8 is as low as 2-8 mu g / mL and is superior to that of The Peptide 2 and the Peptide 7 belong to strain specific antibacterial peptides, and are highly specific to bacillus subtilis. The antibacterial peptide has a wide application prospect in the field of preparation of antibacterial dressings and antibacterial drugs.

The invention discloses acinetobacter baumannii and application of the acinetobacter baumannii in construction of a mastitis animal model. The acinetobacter baumannii strain is acinetobacter baumannii AbST3475NMG202308, and the preservation number of the acinetobacter baumannii strain is CGMCC (China General Microbiological Culture Collection Center) No.34450 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms). Experiments prove that the acinetobacter baumannii has high pathogenicity and can be used for establishing a typical animal model caused by acinetobacter baumannii infection, the animal model can be specifically a mastitis animal model, and a certain technical support is provided for subsequent research and development of drugs or vaccines; the acinetobacter baumannii can also be used for screening antibacterial drugs and developing diagnostic reagents and vaccines. The method has an important application value.

The invention discloses an application of NSC348884 in preparation of an antibacterial drug, the bacteria of the NSC348884 are gram-negative bacteria or drug-resistant gram-negative bacteria, and the gram-negative bacteria are acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa or klebsiella pneumoniae; based on an antibacterial drug high-throughput screening technology, it is found from 1280 small molecule compounds that the benzimidazole compound NSC348884 has direct bacteriostasis and sterilization effects on gram-negative bacteria and drug-resistant gram-negative bacteria, and no drug resistance is generated after the benzimidazole compound NSC348884 is continuously applied for 14 days. Further research shows that NSC348884 can interfere with lipid synthesis of gram-negative bacterium cell membranes, inhibit formation of biological membranes and induce lipid metabolism disorder, so that bacteriostatic and bactericidal effects are achieved. In addition, the NSC348884 can also enhance the sensitivity of the drug-resistant bacteria to the imipenem and the polymyxin B.

The invention provides a method for preparing a degradable bone-guided regeneration membrane with an asymmetric membrane layer, which comprises the following steps of: cutting a magnesium metal sheet into a magnesium net or a magnesium bracket with small holes by laser, carrying out heat treatment on the magnesium net or the magnesium bracket by using HF (Hydrogen Fluoride) to obtain a layer of MgF4 coating on the surface, and immersing the magnesium net or the magnesium bracket into a macromolecular solution until a solvent of the macromolecular coating is completely volatilized; then preparing an electrostatic spinning membrane on the surface of the sample: preparing a dense polymer membrane layer containing an antibacterial drug on the surface of the sample close to one side of the fiber tissue by adopting a hot pressing method, or preparing a dense electrostatic spinning ordered fiber membrane which contains an antibacterial nano-drug and is arranged in parallel by adopting an electrostatic spinning method; the designed structure and the used materials can realize an antibacterial function and shield fiber tissues from growing in. The nanofiber membrane which contains osteogenesis promoting substances such as nano-hydroxyapatite or nano-strontium and is conveyed disorderly is prepared on the side close to the bone tissue by adopting an electrostatic spinning technology, and the designed structure and the contained substances can promote osteogenesis.

The invention relates to lactobacillus mucilaginosus and application thereof. Wherein the gene of the molecular marker for fermenting lactobacillus mucus has one of the following nucleotide sequences: (1) a nucleotide sequence as shown in SEQ ID NO: 24; (2) a nucleotide sequence having at least 97%, 98%, 99% or higher homology with the nucleotide sequence as shown in SEQ ID NO: 24; and (3) a nucleotide sequence with one or more nucleotide sequences, such as 1, 2, 3, 4, 5 or more nucleotide substitutions, deletion or insertion, in the nucleotide sequence as shown in SEQ ID NO: 24. The fermented lactobacillus mucus provided by the invention is non-toxic, has good biological characteristics, has good curative effects on prevention and / or treatment of vaginal infection and related diseases, and avoids the problems of increased drug resistance, high recurrence rate and the like caused by use of antibiotic and other antibacterial drugs during treatment of vaginal infection and related diseases.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide cAMP-1116nc with a broad-spectrum antibacterial effect and application of the antibacterial peptide cAMP-1116nc. The antibacterial peptide cAMP-1116nc is obtained by carrying out N-terminal acetylation modification and C-terminal amidation modification on a polypeptide as shown in SEQ ID NO. 1. The antibacterial peptide cAMP-1116nc is an antibacterial peptide cAMP-1116nc. The antibacterial peptide cAMP-1116nc disclosed by the invention shows remarkable antibacterial activity in a plurality of gram-positive bacteria and gram-negative bacteria. The antibacterial peptide cAMP-1116nc can efficiently kill clinically common gram-negative pathogenic bacteria acinetobacter baumannii under the conditions that the pH is equal to 2, the pH is equal to 7.4 and the pH is equal to 8.4, has relatively low cytotoxicity, has the potential of preparing antibacterial drugs, and is suitable for developing antibacterial products with multiple purposes, including the antibacterial drugs, disinfectants and biological preservatives. The invention provides a novel functional polypeptide for the field of antibacterial peptides, and the novel functional polypeptide has important practical application value.

The invention provides an antibacterial peptide derived from zebra fish, the amino acid sequence of the antibacterial peptide is SEQ ID NO: 1, and the sequence of coding nucleotide of the antibacterial peptide is SEQ ID NO: 2. The antibacterial peptide provided by the invention can be used for preparing products for preventing, treating and resisting bacteria. The zebrafish antibacterial peptide mP60-81 disclosed by the invention has a remarkable antibacterial effect on gram-positive bacteria and gram-negative bacteria, is wide in antibacterial spectrum, has great use value, and has better application in the aspect of developing and preparing antibacterial agents. The antibacterial peptide is derived from zebra fish, can be applied to aquaculture as a feed additive, and can also be developed into an antibacterial agent, an antibacterial drug and the like, so that the antibacterial peptide has a wide application prospect.

The invention discloses a broad-spectrum antibacterial peptide as well as a screening method and application thereof, and belongs to the technical field of antibacterial peptides, a bioinformatics method is mainly adopted for searching a new antibacterial peptide, then amino acid sequence optimization is carried out, and finally the antibacterial peptide with a better antibacterial effect is obtained. Sterilization activity evaluation is carried out by using various known strains with drug resistance, and the result shows that the antibacterial peptide provided by the invention has broad-spectrum antibacterial activity on drug-resistant bacteria, more comprehensive antibacterial effect and wider indications; the compound has the advantages of no hemolysis, low cytotoxicity, efficient in-vivo antibacterial activity and high stability, and has application safety; the compound has a wide application prospect of being developed into a human body antibacterial drug.