Screening method of 79 antibacterial agents in animal foods

A technology of animal food and antibacterial drugs, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as lack, achieve the effect of improving work efficiency and shortening the detection cycle

Inactive Publication Date: 2017-10-03
河南省兽药饲料监察所
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  • Abstract
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  • Claims
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Problems solved by technology

At present, domestic methods for the simultaneous determination and accurate quantification of multi-drug residues in animal products are relatively lacking, so it is urgent to establish a detection technology for simultaneous determination of multi-drug residues

Method used

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  • Screening method of 79 antibacterial agents in animal foods
  • Screening method of 79 antibacterial agents in animal foods
  • Screening method of 79 antibacterial agents in animal foods

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Experimental program
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Embodiment Construction

[0135] A total of 13 categories of 79 kinds of antibacterial drugs were tested, including:

[0136] (a) Sulfonamides: sulfamethoxine, sulfamethoxazole, sulfamethoxine, sulfaquinoxaline, sulfamethazine, sulfachloropyridazine, sulfapyridine, sulfamethoxazole, sulfamethiazine Oxadiazole, sulfamethoxazole, sulfachlorpyrazine, sulfamethoxine, sulfadimethoxine, sulfadiazine, sulfamerazine, sulfisoxazole, benzosulfonamide, sulfamethoxine, sulfathiazole ;

[0137] (b) Cephalosporins: cefquinome;

[0138] (c) Tetracyclines: oxytetracycline, tetracycline, aureomycin;

[0139] (d) Amino alcohols: chloramphenicol, florfenicol, thiamphenicol;

[0140] (e) Macrolides: Tylosin, Tilmicosin, Azithromycin, Clarithromycin, Roxithromycin, Kitamycin A5;

[0141] (f) Lincosamides: lincomycin, clindamycin;

[0142] (g) Fluoroquinolones: enrofloxacin, ciprofloxacin, sarafloxacin, difloxacin, nafloxacin, norfloxacin, pefloxacin, nalidixic acid, cinoxacin, Lomefloxacin, ofloxacin, oxolinic acid, ...

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Abstract

The invention discloses a screening method of 79 antibacterial agents in animal foods. The screening method comprises preparation of a standard working solution, analysis pretreatment on a sample to be tested, drawing of a standard curve, qualitative analysis and quantitative analysis. A high throughput screening and quantitative detection platform is built so that the detection of a single compound is developed into the simultaneous detection of a plurality of compounds in the field. Compared with the prior art, the screening method realizes a detection cost about 1 / 2 the original cost, shortens a sample detection period by 1 / 2 and greatly improves work efficiency. The screening method plays an effective role in the veterinary drug residue monitoring program, the pollution-free animal product testing program and the national animal product routine testing program implemented by the Ministry of Agriculture.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a screening method for 79 kinds of antibacterial drugs in animal food. Background technique [0002] In recent years, my country's animal husbandry has become an important pillar industry of agriculture and has made great achievements, which is inseparable from the development and rational use of veterinary drugs. Veterinary drugs are important substances for the prevention, diagnosis and treatment of animal diseases. When used properly, they can reduce the morbidity and mortality of livestock and poultry, promote animal growth, improve animal product quality and increase feed utilization. Commonly used veterinary drugs in clinical practice include fluoroquinolones, sulfonamides, macrolides, tetracyclines, lincosamines, etc. However, the abuse of drugs in the treatment and prevention of animal diseases is relatively common at present, and the dosage of so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 吴宁鹏吴志明孟蕾耿雨婕宋志超宋善道宋宇方忠意杜红鸽朱雷张崇威
Owner 河南省兽药饲料监察所
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