2230 results about "Tissue culture" patented technology

According to the disclosure, there is provided seed and plants of the corn variety designated CV960136. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV960136, and to methods for producing a corn plant produced by crossing a corn plant of variety CV960136 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV960136 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV960136.

According to the disclosure, there is provided seed and plants of the corn variety designated CV962284. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV962284, and to methods for producing a corn plant produced by crossing a corn plant of variety CV962284 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV962284 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV962284.

According to the invention, there is provided seed and plants of the corn variety designated CV554903. The invention thus relates to the plants, seeds and tissue cultures of the variety CV554903, and to methods for producing a corn plant produced by crossing a corn plant of variety CV554903 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV554903 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV554903.

According to the disclosure, there is provided seed and plants of the corn variety designated CV963205. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV963205, and to methods for producing a corn plant produced by crossing a corn plant of variety CV963205 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV963205 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV963205.

According to the disclosure, there is provided seed and plants of the corn variety designated CV964750. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV964750, and to methods for producing a corn plant produced by crossing a corn plant of variety CV964750 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV964750 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV964750.

According to the disclosure, there is provided seed and plants of the hybrid com variety designated CH010478. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010478, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010478 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010478.

According to the disclosure, there is provided seed and plants of the corn variety designated CV951892. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV951892, and to methods for producing a corn plant produced by crossing a corn plant of variety CV951892 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV951892 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV951892.

The invention relates to the technical field of plant tissue culture, in particular to a Rhododendron pulchrum tissue culture method based on callus regeneration. According to the method, the tender cotyledons of aseptic seedlings are taken as explants, and an efficient Rhododendron pulchrum tissue culture and rapid propagation technical system is established through induction of calluses, subculture multiplication of the calluses, differentiation of adventitious buds, bud strengthening and rooting culture, so that complete tissue culture progeny plants are formed. The induction rate of the callus tissue of the system is 100.0%, the proliferation multiple of the callus tissue in a subculture cycle reaches 5.2 times, 28.5 adventitious buds can be differentiated from a callus tissue block with the size of about 0.5 mm * 0.5 mm * 0.5 mm, the rooting rate reaches 95.8%, the system is not limited by factors such as seasons, climate and regions, and efficient and rapid propagation can be achieved all the year round. Meanwhile, the establishment of the system also lays a technical foundation for genetic transformation improvement, mutant screening breeding and the like of the Rhododendron pulchrum based on the callus.

According to the disclosure, there is provided seed and plants of the corn variety designated CV972960. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV972960, and to methods for producing a corn plant produced by crossing a corn plant of variety CV972960 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV972960 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV972960.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010491. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010491, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010491 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010491.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010511. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010511, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010511 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010511.

The invention discloses a large-scale preparation method of ginseng exosomes, which comprises the following steps: culturing calluses: inoculating explants into an induction culture medium until ginseng calluses are generated; proliferation of the calluses: inoculating the newborn calluses into a proliferation culture medium for proliferation culture; inducing germination and rooting: after the ginseng callus is amplified, transplanting the ginseng callus into a germination induction culture medium until the ginseng callus is germinated, and then transferring the ginseng callus into a rooting culture medium for culturing and rooting; and preparing the exosome, namely grinding root hairs grown from the ginseng callus regeneration plant, uniformly mixing the ground root hairs with PBS (Phosphate Buffer Solution) or performing liquid culture on ginseng suspension cells, and finally performing centrifugal treatment to obtain the ginseng exosome. According to the large-scale preparation method of the ginseng exosome, the endotoxin pollution in the growth and development process is reduced through ginseng callus culture rooting, and then the ginseng exosome is prepared, so that the large-scale preparation requirement in modern production and processing is met.

The invention relates to the technical field of plant stress resistance screening, in particular to a method for screening drought-resistant mutants after EMS mutagenesis of bamboo reed cluster buds, which comprises the following steps: S1, performing hydroponic seedling strengthening on wild tissue culture seedlings and EMS-treated rooting tissue culture seedlings to obtain wild bamboo reed seedlings and EMS-treated bamboo reed seedlings; s2, screening suitable concentration of PEG-6000: screening wild type bamboo reed seedlings by using a series of PEG-6000 solutions with different concentrations, comparing survival rates of the bamboo reed seedlings under different treatments, and screening suitable concentration for simulating drought stress by a PEG-6000 water culture method; s3, simulating drought stress conditions by using the screened PEG-6000 solution with proper concentration as a permeable medium, and screening the EMS treated bamboo reed seedlings to obtain drought-resistant plants. The screening method provided by the invention is not limited by seasons, has high accuracy, and is a simple, convenient and rapid method for screening drought-resistant bamboo reed plants.

The invention discloses a potato cold resistance identification method and system, and relates to the technical field of cold resistance identification. The method comprises the following steps: cultivating potato tissue culture seedlings in a standardized manner, and randomly dividing the seedlings into a low-temperature stress group and a normal-temperature control group; performing standardized low-temperature treatment including slow descent and constant-temperature stages on the stress group; the method comprises the following steps: acquiring multi-source data such as chlorophyll fluorescence parameters, canopy thermal imaging, digital image phenotypes and biochemical indexes, and forming a unified data set after derivative index calculation and standardized fusion; inputting the fusion data into an improved neural network model, training the model through a particle swarm optimization algorithm, and outputting a quantitative potato cold resistance index; automatically evaluating the cold resistance grade according to the potato cold resistance index and generating an identification report; standardization of the identification process, fusion analysis of multi-dimensional indexes and intelligent result analysis are achieved, and the accuracy, efficiency and practicability of potato cold resistance identification are remarkably improved.

The invention discloses a rapid spore propagation method of a cyatheridae rare fern type pteridium aquilinum var. Spinulosa var. Spinulosa. Comprising the following steps: (1) collecting and storing spores; (2) selecting and preparing a spore culture medium; (3) spore treatment and sowing; (4) culturing spores; (5) observing and maintaining gametophytes; (6) transplanting sporophyte young plants; (7) seedling management and protection; (8) transplanting seedlings. When seedlings in the seedling raising box grow to about 5-8 cm high, the seedling raising box is moved into a non-imitation cloth seedling raising bag in an outdoor greenhouse nursery. Compared with tissue culture, the method has the advantages of high success rate, simplicity and high efficiency. A manual climatic box is not needed in a spore-gametophyte cultivation stage, but on a light-cared cultivation frame, so that the operation is simple and easy, and the limitation of space is avoided.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010529. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010529, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010529 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010529.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010438. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010438, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010438 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010438.

The invention discloses a tissue culture method of a citral type cinnamomum bodinieri stem with an axillary bud, and relates to the technical field of forest tree tissue culture. The tissue culture method comprises the following steps: selecting a current-year semi-lignified stem on a citral type cinnamomum bodinieri excellent single plant for disinfection treatment, and inoculating the stem on an axillary bud primary induction culture medium for axillary bud primary induction culture; after stem axillary buds in the axillary bud primary induction culture medium germinate, stem segments with axillary buds are soaked with a phycocyanobilin solution and then inoculated into the adventitious bud induction culture medium, red light irradiation treatment is conducted, conventional adventitious bud induction culture is conducted after red light irradiation, adventitious bud subculture multiplication culture, strong seedling culture and rooting induction culture are conducted, and the stem segments with the axillary buds are obtained. And finally, hardening seedlings and transplanting. According to the tissue culture method provided by the invention, the adventitious bud multiplication coefficient can reach more than 5, the rooting rate reaches up to 90.00%, the average rooting number also reaches 3.44, and the survival rate after two months is more than 85%. The invention provides support for better development and utilization of citral type Cinnamomum bodinieri, and has important significance for popularization and application of citral type Cinnamomum bodinieri.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010528. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010528, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010528 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010528.

The invention provides a tissue protection solution and application thereof. The tissue protection liquid comprises a basic culture medium; vitamins, wherein the vitamins comprise vitamin E; the antioxidant is prepared from at least one of N-acetylcysteine amide, superoxide dismutase and catalase; and a specific cell factor, wherein the specific cell factor comprises at least one of a ROCK inhibitor Y27632 and insulin. Therefore, when the tissue protection liquid is used for storing and transporting a tissue sample, the loss in the process from tissue sample separation to experiment starting can be reduced, the function and activity of tissue cells are ensured, and the success rate of establishment of various primary tissue cultures is effectively improved.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010457. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010457, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010457 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010457.