13180 results about "Tissue culture" patented technology

An method and method for generating an electrical signal for use in biomedical applications, including two timing-interval generators, each optionally driving a multistep sequencer; analog, digital or hybrid means for combining the resulting timed signals into a complex electrical signal; optional filtering means for blocking direct current, removing selected frequency components from the resulting signal, and / or providing voltage step-up if needed; and conductive means for coupling the resulting signal to a human or animal body, food, beverage or other liquid, cell or tissue culture, or pharmaceutical material, in order to relieve pain, stimulate healing or growth, enhance the production of specific biochemicals, or devitalize selected types of organisms.

According to the invention, there is provided seed and plants of the corn variety designated I285291. The invention thus relates to the plants, seeds and tissue cultures of the variety I285291, and to methods for producing a corn plant produced by crossing a corn plant of variety I285291 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety I285291 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety I285291.

According to the invention, there is provided seed and plants of the corn variety designated CV952947. The invention thus relates to the plants, seeds and tissue cultures of the variety CV952947, and to methods for producing a corn plant produced by crossing a corn plant of variety CV952947 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV952947 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV952947.

According to the invention, there is provided seed and plants of the corn variety designated CV112211. The invention thus relates to the plants, seeds and tissue cultures of the variety CV112211, and to methods for producing a corn plant produced by crossing a corn plant of variety CV112211 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV112211 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV112211.

According to the invention, there is provided seed and plants of the corn variety designated I351729. The invention thus relates to the plants, seeds and tissue cultures of the variety I351729, and to methods for producing a corn plant produced by crossing a corn plant of variety I351729 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety I351729 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety I351729.

A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and / or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.

The present invention relates to a method for isolating stem / progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem / progenitor cells from the tissue cultures. The isolated stem cell cells can have embryonic stem cell-like properties and can be used for various therapeutic purposes. In one embodiment, the invention relates to the isolation and cultivation of stem cells such as epithelial and / or mesenchymal stem / progenitor cells under conditions allowing the cells to undergo mitotic expansion. Furthermore, the invention is directed to a method for the differentiation of the isolated stem / progenitor cells into epithelial and / or mesenchymal cells.

The invention provides seed and plants of the lettuce line designated PS 06518893. The invention thus relates to the plants, seeds and tissue cultures of lettuce line PS 06518893, and to methods for producing a lettuce plant produced by crossing a plant of lettuce line PS 06518893 with itself or with another lettuce plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of lettuce line PS 06518893, including the gametes of such plants.

According to the invention, there is provided seed and plants of the corn variety designated CV847814. The invention thus relates to the plants, seeds and tissue cultures of the variety CV847814, and to methods for producing a corn plant produced by crossing a corn plant of variety CV847814 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV847814 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV847814.

According to the invention, there is provided seed and plants of the corn variety designated CV995128. The invention thus relates to the plants, seeds and tissue cultures of the variety CV995128, and to methods for producing a corn plant produced by crossing a corn plant of variety CV995128 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV995128 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV995128.

A novel cotton variety, designated as FM 991B2R, is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by crossing plants of variety FM 991B2R with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of FM 99IB2R and to plants of FM 991B2R reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 991B2R.

Connective tissue, including neo-tendons and ligaments, has been constructed using biodegradable synthetic scaffolds seeded with tenocytes. The scaffolds are preferably formed from biodegradable fibers formed of a polymer such as polyglycolic acid-polylactic acid copolymers, and seeded with cells isolated from autologous tendon or ligament by means of enzymatic digestion or direct seeding into tissue culture dishes from explants. The cell polymer constructs are then surgically transplanted to replace missing segments of functioning tendon or ligament.

According to the invention, there is provided seed and plants of the corn variety designated I325350. This invention thus relates to the plants, seeds and tissue cultures of the variety I325350, and to methods for producing a corn plant produced by crossing a corn plant of variety I325350 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I325350 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I325350, and also to the SSR and isozyme typing profiles of corn variety I325350.

Powder type culture medium is from plenty of element kinds , trace element and iron salt and vitamin kind of composition, after processing manufacture the original powder that makes powder culture medium. The various components of the medium and the final product were dry powder. The preparation Method: First, concentrate all plenty of elements to collect reserve; Mix trace element and vitamin kind totally to wrap to attach , and then mix the thin medicine and plenty of elements of trace element totally, under the role with centrifugal high speed, making it even scatter and wrap up to glue to unite. So, make the original powder of powder culture medium for manufacture. This medium-dry powder manufacturing easier, does not require the use of complex machines and equipment, and low-cost easy to promote. The product is of high quality in existing product. It can be used in plant breeding, tissue culture and molecular biology research, and can be used for various commercial purposes.

The present invention relates to a skin equivalent and a method for producing the same, wherein the skin equivalent comprises a scaffold and stem / progenitor cells isolated from the amniotic membrane of umbilical cord. These stem / progenitor cells may be mesenchymal (UCMC) and / or epithelial (UCEC) stem cells, which may then be further differentiated to fibroblast and keratinocytes. Further described is a method for isolating stem / progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem / progenitor cells from the tissue cultures. The invention also refers to therapeutic uses of these skin equivalents. Another aspect of the invention relates to the generation of a mucin-producing cell using stem / progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such mucin-producing cells. In yet another aspect, the invention relates to a method for generating an insulin-producing cell using stem / progenitor cells isolated from the amniotic membrane of umbilical cord and therapeutic uses thereof. The invention further refers to a method of treating a bone or cartilage disorder using UCMC. Furthermore, the invention refers to a method of generating a dopamin and tyrosin hydroxylase as well as a HLA-G and hepatocytes using UCMC and / or UCEC. The present invention also refers to a method of inducing proliferation of aged keratinocytes using UCMC.

The present invention provides defined serum-free cell culture media useful in culturing fibroblasts, especially articular chondrocytes, that avoids problems inherent in the use of serum-containing media. The defined media comprise platelet-derived growth factor (PDGF), and chemically defined lipids, or combinations of these compounds. In another aspect, the present invention also provides tissue culture methods that comprise incubating chondrocytes in the defined serum free media. The methods enhance attachment and proliferative expansion of chondrocytes seeded at low density while maintaining their redifferentiation potential.

According to the invention, there is provided seed and plants of the corn variety designated I226218. The invention thus relates to the plants, seeds and tissue cultures of the variety I226218, and to methods for producing a corn plant produced by crossing a corn plant of variety I226218 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety I226218 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety I226218.

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH093858. The invention thus relates to the plants, seeds and tissue cultures of the variety CH093858, and to methods for producing a corn plant produced by crossing a corn plant of variety CH093858 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH093858.

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH041570. The invention thus relates to the plants, seeds and tissue cultures of the variety CH041570, and to methods for producing a corn plant produced by crossing a corn plant of variety CH041570 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH041570.

A method for preparing a tissue culture insert that is used for constructing a transplantable graft of an engineered tissue equivalent comprising living main functional cells, stromal cells and tissue matrix on / in a biological supporting membrane for treatment of body tissue defects.

The invention relates to a bioreactor comprising a chamber for containing cells or tissue cultures within a culture medium. The bioreactor also comprises a detector capable of detecting a change in one or more metabolites associated with growth of the cell or tissue cultures within the chamber and a chamber drive capable of rotating the chamber at a first speed about a first axis and a second speed about a second axis, the second axis being disposed at an angle relative to the first axis. In use, the magnitude of the first speed and the second speed are independently variable of each other.

Described herein are environmentally isolated tissue culture devices that may be used for cell culture, as well as systems including these devices and methods for using them. These devices may include control features for regulating the micro-environment within a well or wells of the device. For example, on-board features may regulate the temperature, humidity, pH, media level, media composition, CO2 / O2 / N2 levels, drug concentration, cell density, byproduct (or product) production, and mixing of materials within the chamber. Material may be added to or withdrawn from the wells of the device without opening the device. Also described herein are controllers for analyzing and controlling the micro- environment within the well. Thus, the plates described herein may be used without requiring a separate incubator, allowing cells to be analyzed (e.g., imaged) continuously, allowing real-time reactions while monitoring under a microscope for hours, days or even weeks.

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH110748. The invention thus relates to the plants, seeds and tissue cultures of the variety CH110748, and to methods for producing a corn plant produced by crossing a corn plant of variety CH110748 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH110748.

According to the invention, there is provided seed and plants of the corn variety designated I180580. This invention thus relates to the plants, seeds and tissue cultures of the variety I180580, and to methods for producing a corn plant produced by crossing a corn plant of variety I180580 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I180580 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I180580, and also to the SSR and isozyme typing profiles of corn variety I180580.