The invention discloses a
tissue culture method for bulbils of brachystemma chinense, and belongs to the technical field of
pteridophyte propagation and cultivation. The method specifically comprises the following steps: (1) selecting an explant, namely selecting mature bulbils of brachygyne tenuifolia as the explant; (2) disinfecting the explants, and washing bulbils of the
phoenix dactylifera under
tap water; placing the washed bulbils in a tea bag sterilized at high temperature, disinfecting with a
mixed solution, and rinsing with
sterile water; (3) preparing a sterile
solid culture medium, adopting the culture medium, adding
agar, adjusting the pH value, sterilizing at high temperature, and cooling to obtain the sterile
solid culture medium; (4) inoculating and culturing, namely inoculating the bulbils of the phoenix tree into a sterile
solid culture medium, and culturing the bulbils of the phoenix tree in an illumination
incubator to obtain seedlings of the phoenix tree; and (5) transferring the seedlings, wherein the seedlings can be transferred after the seedlings are observed to completely expand leaves. According to the method, the culture period of the david
fern is shortened, the method is not limited by seasons, and
tissue culture seedlings are formed in one step, so that rapid propagation is realized, the cost is reduced, and the consistency and health degree of
plant growth are enhanced.