397 results about "Angiogenesis" patented technology

The invention is applicable to the technical field of hydrogel, and provides dynamic cross-linked hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: cross-linking oxidized dextran and carboxymethyl chitosan containing phenylboronic acid modification groups; glucanase is added before the hydrogel is formed, and calcium peroxide nanoparticles coated with polydopamine are introduced; wherein the mass concentration of the oxidized dextran is 2-10% w / v, the mass concentration of the carboxymethyl chitosan containing the phenylboronic acid modified group is 2% w / v, the concentration of the glucanase is 10-100 U, and the concentration of the calcium peroxide nanoparticles coated with the polydopamine is 0.1-1% w / v. The hydrogel disclosed by the invention shows good cell compatibility, angiogenesis promoting capability and bioactivity for promoting dental pulp regeneration in multiple in-vitro and in-vivo experiments, is suitable for tissue engineering fields such as dental pulp injury repair and the like, and effectively solves the technical problem that conventional hydrogel is insufficient in cell survival support in three-dimensional culture and the like.

The invention belongs to the technical field of medical gel, and particularly relates to modified hydrogel for skin wound healing and a preparation method thereof.The modified hydrogel is prepared from, by weight, 2-5 parts of drug-loaded GDVLNs, 1-2 parts of chitosan, 0.6-1.5 parts of antioxidant, 0.2 part of LAP, 1.5 parts of MA, 18 parts of gel matrix and 100 parts of buffer solution, the drug-loaded GDVLNs prepared from the traditional Chinese medicine active ingredients and the ginseng vesicles have the functions of resisting oxidative stress, promoting angiogenesis, promoting cell proliferation and the like, and the MA modified hydrogel system can realize slow release and controlled release of the drug-loaded GDVLNs and play a long-acting healing promoting role.

The invention discloses an adipose-derived stem cell exosome compound loaded with silver nanoparticles and application of the adipose-derived stem cell exosome compound loaded with the silver nanoparticles, and ADSC-Exos loaded with the silver nanoparticles is developed by integrating AgNPs into an exosome of ADSCs. In vivo, the Exo-AgNPs can accelerate healing of infected wounds by reducing bacterial colonization and promoting collagen deposition, angiogenesis and M2 type macrophage polarization. In vitro, the Exo-AgNPs activates the functions of fibroblasts and vascular endothelial cells, and shows an excellent antibacterial property. In addition, the Exo-AgNPs induces polarization of M2 type macrophages and inhibits secretion of proinflammatory cytokines by activating autophagy. The results show that Exo-AgNPs is expected to become an ideal biological material for treating infected wounds.

The invention belongs to the technical field of nano-drugs, and discloses a multifunctional nano-drug delivery system for treating neovascular eye diseases and a preparation method of the multifunctional nano-drug delivery system. The nano drug delivery system disclosed by the invention is a composite nano preparation which takes dendritic mesoporous silica nanoparticles as a carrier and co-loads cerium zirconium oxide nano enzyme and an anti-VEGF (vascular endothelial growth factor) drug, various active oxygen can be efficiently removed, oxygen is continuously released through catalytic decomposition of hydrogen peroxide, a retina hypoxia microenvironment is improved, and the retina hypoxia effect is improved. According to the present invention, the anti-VEGF drug delivery system can reduce the oxidative stress induced retinal pigment epithelial cell apoptosis rate, reduce the inflammatory reaction, and simultaneously achieve the slow release and the controlled release of the anti-VEGF drug, and the drug effect maintenance time can achieve more than 60 days, such that the multi-target synergistic treatment can be achieved through the synergistic regulation of the multiple pathological links such as oxygen deficit-oxidative stress-inflammation-angiogenesis, and the anti-VEGF drug delivery effect can be achieved. The choroidal neovascularization can be effectively inhibited by single intravitreal injection, and a new strategy of multi-target collaborative treatment is provided for neovascular macular degeneration and other eye diseases.

The invention discloses a magnetoelectric stent for realizing bone repair under external field driving and a preparation method and application thereof.The stent comprises a stent body based on a triple-period minimal curved surface structure and a magnetoelectric nano-particle coating, and the stent body comprises a convoluted and / or through hole structure; and the magnetoelectric nanoparticle coating is loaded on the inner surface of at least part of the pore structure. The magnetoelectric stent is suitable for providing better mechanical support for the early stage of bone repair of large-area bone defects, meanwhile, under the synergistic effect of magnetoelectric stimulation, osteogenesis and angiogenesis coupling can be promoted, and bone defect repair under the complex conditions of oral clinical alveolar bone vertical bone increment and systemic diseases such as inflammation and the like is achieved.

The invention provides a hydrogel dressing for synergistically promoting chronic wound healing through near-infrared controlled release of ROS (reactive oxygen species) and NO (nitric oxide) as well as a preparation method and application of the hydrogel dressing. Belongs to the technical field of biomedical materials. The hydrogel is prepared from the following components: upconversion nanoparticles as a core, silicon dioxide wrapping, carbon dot and L-arginine loading, maltotriose modification, and mixing and gelling with 2-amino-2-fluoro-2-deoxyadenosine as a hydrogel carrier, so as to obtain the hydrogel dressing. The obtained hydrogel dressing has the functions of inhibiting bacteria, promoting cell proliferation, promoting revascularization and the like, and remarkably promotes skin wound healing.

The invention provides application of injectable hydrogel in preparation of a medicine for treating myocardial infarction. The injectable hydrogel is obtained by mixing aniline tetramer grafted gelatin, adipic dihydrazide modified hyaluronic acid and an aldehyde group-containing metal polyphenol complex. The injectable hydrogel has antioxidant activity, conductivity and angiogenesis promoting activity performance, and myocardial repair is promoted through synergistic regulation and control.

The invention relates to the technical field of biological materials, in particular to composite hydrogel as well as a preparation method and application thereof. In particular to an ion response type ELPs-SA composite hydrogel constructed based on elastin-like polypeptides (ELPs) and sodium alginate (SA) as well as a preparation method and application of the ion response type ELPs-SA composite hydrogel. According to the hydrogel, elastic-like polypeptides ELP-Vs (such as adhesion promotion, angiogenesis, antibiosis and the like) with biological functions are introduced and react with SA, CaCO3 and gluconolactone (GDL) together, and the non-covalent cross-linked three-dimensional network hydrogel is formed under mild conditions. The obtained hydrogel has excellent mechanical strength (the energy storage modulus is 450-1773 Pa), a proper microporous structure (the pore diameter is 50-100 microns) and good biocompatibility, and can support three-dimensional culture of cells and promote angiogenesis and immunoregulation. The material has a wide application prospect in the biomedical fields of tissue engineering, wound repair, organoid construction and the like.

The invention relates to the technical field of biomedical materials, in particular to a degradable zinc-based composite GBR film with the surface loaded with a nanometer electrostatic spinning fiber coating and preparation and application of the degradable zinc-based composite GBR film. According to the degradable zinc-based composite GBR membrane with the surface loaded with the nano electrostatic spinning fiber coating, the nano fiber coating is implanted into the surface of a zinc-based material such as zinc alloy by adopting an electrostatic spinning technology, and the degradable zinc-based composite GBR membrane with the surface loaded with the nano electrostatic spinning fiber coating can inhibit excessive release of zinc ions, improve biocompatibility of the zinc alloy and the like, promote osteoblast differentiation and promote angiogenesis. The electrostatic spinning coating on the surface of the zinc-based composite GBR membrane serves as a physical barrier to block invasion of epithelial cells and fibrocytes, and angiogenesis is promoted along with degradation of fibers and slow release of drugs; the porous zinc alloy and the like in the middle layer not only provide mechanical support, but also release zinc ions in the degradation process to achieve multiple functions of resisting bacteria and promoting osteogenesis.

The invention relates to a dressing for promoting healing of diabetic wounds. Chitosan is dissolved in a hydrochloric acid solution at room temperature to obtain a chitosan solution; the preparation method comprises the following steps: dissolving loxoprofen in ethanol activated by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide / N-hydroxysuccinimide, dropwise adding into a chitosan solution, adding ethanol while stirring at a high speed by using a magnetic rod to form a transparent solution, and stirring at a low speed in the dark; air-drying the solution in a drying oven to form a solid film, and thoroughly washing the solid film in ethanol to obtain a chitosan-loxoprofen conjugate; the preparation method comprises the following steps: adding chitosan-loxoprofen into a phosphate buffer solution to form a chitosan-loxoprofen solution; dissolving genipin in a phosphate buffer solution to obtain a genipin solution, stirring and mixing the chitosan-loxoprofen solution and the genipin solution at a high speed, cross-linking, and freezing in a refrigerator to obtain the chitosan-loxoprofen / genipin sponge bracket. The dressing disclosed by the invention can effectively relieve chronic inflammation, remarkably improve skin tissue regeneration and stimulate angiogenesis.

The present application relates to the technical field of medicine, in particular to the application of gansix triol A in the preparation of a drug for treating gastric cancer and / or non-small cell lung cancer, gansix triol A plays an anti-gastric cancer role by selectively inhibiting the proliferation of gastric cancer MGC-803 cells and inhibiting angiogenesis, the mechanism involves inhibiting the VEGF / VEGFR2 signal pathway and inducing cell apoptosis; the mechanism of gansix triol A in inhibiting the VEGF / VEGFR2 signal pathway is similar to that of bevacizumab; gansix triol A plays an anti-non-small cell lung cancer role by selectively inhibiting the proliferation of non-small cell lung cancer A549 cells, the mechanism involves inhibiting the MAPK / ERK signal pathway and inducing cell apoptosis. The present application finds that the cytotoxic activity of gansix triol A on MGC-803 cells and A549 cells is significantly stronger than that of tanshinone IIA; gansix triol A has an anti-tumor effect of "one drug with double targets, double inhibition" and "high efficiency and low toxicity".

The invention discloses a method and a system for predicting preeclampsia risk of a pregnant woman based on computer implementation. The method integrates the concentration level of vitamin D (especially 25-hydroxyvitamin D) in serum of pregnant women, angiogenesis biomarkers (such as sFlt-1 / PlGF ratio), conventional clinical indexes (such as mean arterial pressure and body mass index) and personal medical history data. A machine learning algorithm (such as a random forest or a gradient lifting model) is adopted to carry out weighted analysis on the multi-dimensional data, and a dynamic and high-precision prediction model is constructed. The system comprises a data acquisition module, an intelligent prediction module and a clinical decision support module, and can provide real-time risk assessment and personalized intervention suggestions (such as a vitamin D supplement scheme) for medical staff, so that early warning and effective management of preeclampsia are realized, and the risk of pregnancy outcome caused by maternal and infant poor pregnancy is reduced.

Methods for producing prevascularized organoids, and compositions comprising such prevascularized organoids, are provided. Methods of treating ischemic conditions and promoting angiogenesis by administering the prevascularized organoids to a subject in need thereof are also provided.

The invention relates to the technical field of biomedical materials, in particular to a thermoelectric bionic skin for promoting healing of diabetic wounds by restoring a biological electric field microenvironment, and a preparation method comprises the following steps: adding silver selenide nanoparticles into a 10% methacryloyl gelatin solution, putting the precursor solution into a mold, adding 0.25% phenyl (2, 4, 6-trimethyl-1, 3-pentanedione), and stirring for 20-30 minutes to obtain the thermoelectric bionic skin for promoting healing of diabetic wounds by restoring a biological electric field microenvironment. 2, 6-trimethylbenzoyl) lithium phosphate (LAP) photoinitiator is added, ultraviolet light curing forming is carried out, and after demolding, soaking is carried out to remove residual monomers and the initiator; wherein the drug-containing group is prepared by replacing water with an artemisinin solution, and Ag2Se-coated GelMA or Ag2Se-coated GelMA / ART composite hydrogel is obtained. The electric field generated by the thermoelectric effect in the invention activates a voltage-gated calcium ion channel, inhibits PHD2, and up-regulates an HIF-1 / VEGF-A signal axis, thereby promoting angiogenesis. Meanwhile, the artemisinin also plays an anti-inflammatory role.

The application discloses a kind of hardness correlation liver cancer lung premetastatic niche mouse model construction method, it includes the following steps: (1) in vitro enrichment respectively on the low hardness substrate of hardness 6kPa and the high hardness substrate of hardness 16kPa, the condition culture supernatant of mouse liver cancer cell Hepa1-6 that is cultured and grows, respectively named mL-CM and mH-CM;(2) mL-CM and mH-CM are respectively injected into two groups of mice using tail vein injection mode, every 1 day injection 1 time, until the 26th day, during continuous monitoring the recruitment of BMDCs in fresh lung tissue, while with the induction endpoint, i.e. 26th day premetastatic niche mark feature detection includes lung tissue matrix remodeling, immunosuppression, angiogenesis, vascular permeability and inflammation etc..The method has the advantages of short modeling cycle, easy operation, controllable induction condition, can comprehensively and accurately evaluate the common characteristics of liver cancer lung premetastatic niche formation etc..

This invention provides a bilayer programmed release hydrogel dressing based on PD-168077. The hydrogel dressing is formed by photocrosslinking a lower fast-release layer and an upper slow-release layer. The fast-release layer is formed by photocrosslinking and curing a first pregel solution containing a first concentration of methacrylamide hyaluronic acid, methacrylamide collagen, a photoinitiator, and PD-168077. The slow-release layer is formed by photocrosslinking and curing a second pregel solution containing a second concentration of methacrylamide hyaluronic acid, methacrylamide collagen, a photoinitiator, and PD-168077. This invention also provides a method for preparing the dressing and its application. This invention constructs a biomimetic dermal triple network structure through a photopolymerization strategy of "single formulation, dual concentration, dual curing," achieving programmed release of PD-168077 with a fast-then-slow release. This synergistic effect of the drug, including anti-inflammatory, antioxidant, and angiogenesis-promoting properties, along with matrix bioactivity, effectively promotes the healing of chronic diabetic wounds.

A pharmaceutical composition for prevention or treatment of a disease or disorder characterized by chronic inflammation, associated with angiogenesis and fibrosis. The pharmaceutical composition includes a multi-target inhibitor, multi-phase modulator, or multi-kinase inhibitor, such as axitinib, nintedanib, pirfenidone, riociguat, sorafenib, sunitinib, lenvatinib, regorafenib, ponatinib, or pazopanib.

The present invention belongs to the technical field of recombinant adeno-associated virus (rAAV) gene therapy, relates to the preparation of an rAAV-delivered Fc-engineered VEGF receptor fusion protein or an anti-VEGF antibody, and is applied to the treatment of angiogenesis-related fundus diseases, such as age-related macular degeneration, wet maculopathy, diabetic retinopathy and other diseases. Based on the aflibercept-expressing gene, the present invention uses recombinant AAV delivery to achieve long-term stable expression of a target gene in the RPE layer by means of genetic modification and vector optimization, thereby delivering an optimized target gene sequence to fundus cells of a patient.

The present invention relates to an anti-PTK7 antibody and a use thereof. The anti-PTK7 antibody according to the present invention was found to inhibit angiogenesis, as well as the growth, migration, and invasion of human umbilical vein endothelial cells (HUVECs). Additionally, it effectively suppresses tumor growth in vivo, making it a promising therapeutic agent for angiogenesis-related diseases and PTK7-positive cancers. The antibody also has potential for further development as a targeted therapy for intractable cancers, positioning it as a key global therapeutic agent. Furthermore, the antibody can be humanized for clinical applications and serve as a critical component in the development of novel therapeutics.

The present invention provides novel compounds of Formula (I) and pharmaceutically acceptable salts, solvates, hydrates, tautomers, stereoisomers, isotopically labeled derivatives, and compositions thereof. Also provided are methods and kits involving the compounds or compositions for treating or preventing proliferative diseases in a subject, wherein the proliferative diseases include cancer, benign neoplasm, angiogenesis, inflammatory disease, autoinflammatory disease, and autoimmune disease, and the cancer includes leukemia, melanoma, multiple myeloma. Treatment of a subject having a proliferative disease with the compounds or compositions of the present invention can inhibit abnormal activity of cyclin-dependent kinase 7, thus inducing apoptosis and / or inhibiting transcription in the subject.

Disclosed herein are methods and systems for treating cancer including glioblastoma, recurrent glioblastoma, or newly diagnosed glioblastoma, using the administration of ultra-low radio frequency energy (u / RFE®), either alone or in combination with one or more conventional cancer therapies. In some embodiments, the one or more conventional cancer therapies include chemotherapy or an anti-angiogenic therapy or other therapies.

This invention discloses an alkaloid compound and its uses. Specifically, the invention relates to compounds of formula (I), or stereoisomers, tautomers, nitrides, hydrates, solvates, metabolites, pharmaceutically acceptable salts, or prodrugs of compounds of formula (I), and also to pharmaceutical compositions comprising said compounds and their uses. In particular, this alkaloid compound has a significant inhibitory effect on angiogenesis and can be used as a medicament for the prevention or treatment of tumors, inflammation, ophthalmic diseases, abnormal fibrosis or scar formation, cardiovascular and cerebrovascular diseases, nervous system diseases, infectious diseases, metabolic diseases, and diseases related to abnormal angiogenesis in mammals.

The invention discloses hydrogel for ulcers and a preparation method thereof.The hydrogel is obtained through a cross-linking reaction with polyphenol substance modified gelatin, aldehyde natural polymers and methacrylate gelatin as frameworks, and the hydrogel has injectability and can be injected to oral ulcers or pressure ulcers, and the hydrogel can also be used as a hydrogel for ulcers or oral ulcers or pressure ulcers or oral ulcers or pressure ulcers or pressure ulcers or pressure ulcers or pressure ulcers or pressure ulcers or pressure ulcers or pressure ulcers or pressure ulcers or pressure ulcers or pressure ulcers. The hydrogel can be prepared into a sheet shape to be applied to the ulcer surface, has good biocompatibility, compression resistance, wet adhesion, angiogenesis promotion, hemostasis, inflammation resistance and oxidation resistance, and is beneficial to healing of oral ulcers and pressure ulcers.

The application discloses application of tumor-derived acellular extracellular matrix in preparation of a drug for promoting angiogenesis and tissue regeneration. The application proposes a strategy for regulating tissue regeneration based on a tumor-derived acellular extracellular matrix microenvironment, combines the high similarity between a tumor microenvironment and a regeneration microenvironment such as angiogenesis, and constructs a novel angiogenesis-promoting biomaterial, so that high-efficiency regeneration is realized in various tissue defects. In addition, the characteristics of rapid proliferation of tumor tissues break through the production capacity bottleneck of a raw material source of a traditional acellular extracellular matrix, and provide sustainable biological raw materials for large-scale production. The application successfully solves the problem that a specific tissue-derived acellular extracellular matrix is difficult to regenerate in a complex damage microenvironment.

The invention provides lipid nanoparticles taking zinc phosphate as an inner core, a preparation method of the lipid nanoparticles and a medicine for treating aortic aneurysm, and relates to the technical field of biological medicines. The lipid nanoparticles provided by the invention comprise zinc phosphate nanoparticles, DOPA (Dioctyl-Phthalate Polyamide), ionizable cationic lipid, cholesterol and PEG (Polyethylene Glycol) lipid, the zinc phosphate nano-particles are inner cores of the lipid nano-particles. The lipid nanoparticles have excellent biocompatibility and safety and stable structure, and can improve the stability and delivery efficiency of nucleic acid (such as FBN1mRNA) as a carrier, thereby realizing effective treatment of diseases. According to the medicine for treating the aortic aneurysm, provided by the invention, accurate delivery of FBN1mRNA and expression in FBN1 cells can be realized, and angiogenesis and repair are promoted, so that accurate treatment of the aortic aneurysm is realized.

Cyclic dinucleotides (CDRs) are signaling molecules for cancer therapy, acting as second messengers for intracellular events initiated by GPCR activation and stimulating factors for the interferon gene (STING), thus significantly influencing tumor suppression. Simultaneously, when cancer occurs in a subject, angiogenesis and cell growth proceed at very high rates in vivo, creating a hypoxic environment due to incomplete angiogenesis in cancerous tissue. This environment can be highly conducive to the proliferation of anaerobic bacteria such as Salmonella strains or Escherichia coli. Therefore, this invention relates to a DNA construct incorporating a gene encoding an enzyme for synthesizing cancer therapeutic signaling molecules (e.g., CDRs), or to strains transformed with a vector containing the DNA construct. According to the invention, the DNA construct or strains transformed with a vector containing the DNA construct target cancer in a subject and then secrete C-di-AMP or C-di-GMP synthases in the surrounding environment of the cancer, thus enabling highly effective prevention or treatment of cancer while allowing for real-time cancer diagnosis.

The invention discloses double-response hydrogel as well as a preparation method and application thereof. The double-response hydrogel comprises a three-dimensional porous network hydrogel matrix, and tea polyphenol and nano silicon nitride which are loaded in the hydrogel matrix, the three-dimensional porous network hydrogel matrix is prepared from o-nitrobenzene grafted hyaluronic acid and carboxyl phenylboronic acid modified methyl chitosan. According to the hydrogel disclosed by the invention, in a low-pH / high-ROS microenvironment of a diabetic periodontitis focus, boric acid ester bonds are broken, tea polyphenol is released, and the tea polyphenol plays an anti-inflammatory role by efficiently removing active oxygen and inhibiting inflammatory factors; meanwhile, the Schiff base bond is dissociated to release the nano silicon nitride, and the nano silicon nitride significantly promotes angiogenesis and osteogenic differentiation and accelerates bone defect repair by activating a redox signal, inducing cell autophagy, regulating metabolic reprogramming and other pathways.

The invention provides a composition for chronic wound treatment and a preparation method thereof, and relates to the field of medicines. The mask is prepared from the following raw materials in percentage by mass: 20 to 30 percent of methacrylic acid hyaluronic acid, 20 to 30 percent of metacrylic acid ester modified silk fibroin, 40 to 50 percent of carboxymethyl chitosan, 1 to 2 percent of photoinitiator and 5 to 10 percent of hydrogen sulfide sustained-release microspheres, the hydrogen sulfide sustained-release microsphere is a core-shell microsphere which takes GYY4137-PLGA (poly (lactic-co-glycolic acid)) nanoparticles as a core layer and EGCG (epigallocatechin gallate)-whey protein as a shell layer. The composition provided by the invention can slowly release H2S gas, promote angiogenesis of chronic wounds and promote wound healing.

A scaffold for bone-tissue engineering includes a mesh and a coating on the mesh. The mesh includes a plurality of offset layers of filaments of polyester. The coating covers the mesh and includes magnesium hydroxide / copper oxide nanoparticles suspended in gelatin. A method of constructing the scaffold comprises combining magnesium hydroxide / copper oxide nanoparticles with a gelatin solution and coating a polyester mesh with the combined magnesium hydroxide / copper oxide nanoparticle and gelatin solution.