727 results about "Angiogenesis" patented technology

Hydrogel comprises hydroxyapatite nanowires and a base material for forming the hydrogel, and further comprises glucose oxidase (GOx), gallic acid, magnesium ions (Mg < 2 + >) and the like, the gallic acid and the Mg < 2 + > form Mg-MOF, and the GOx is loaded on the Mg-MOF, so that the diabetes microenvironment of a bone defect part is locally adjusted, and bone defect repair is facilitated. Through verification, the sustained-release gallic acid of GOx-MOF participates in cascade reaction, GOx is removed to generate H2O2, the benefit is maximized, and the sustained-release gallic acid has good effects of controlling blood glucose, removing ROS, regulating inflammation and promoting osteogenesis and angiogenesis. The research is expected to play a role in microenvironment regulation of diabetes mellitus bone regeneration and minimally invasive treatment of bone defects.

The invention provides a recombinant keratin polypeptide as well as a preparation method and application thereof, and relates to the technical field of genetic engineering. The amino acid sequence of the recombinant keratin polypeptide is as shown in any one of SEQ ID No. 1, SEQ ID No. 4, SEQ ID No. 7 and SEQ ID No. 10. The recombinant keratin polypeptide has a good soothing effect and the effects of stimulating skin angiogenesis and improving local blood circulation, and can be widely applied to the industries of medicines, cosmetics, skin care products and the like.

The invention provides a composition for promoting hair regeneration after chemotherapy as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: modifying the surface of a gold nanocage with polydopamine, loading the modified gold nanocage on an exosome, complexing magnesium ions and zinc ions to prepare a modified exosome, preparing a nano drug-loaded liposome from curcumin, resveratrol and epigallocatechin gallate, mixing the nano drug-loaded liposome with the modified exosome to prepare a multi-layer liposome, and preparing the multi-layer liposome from the nano drug-loaded liposome. And mixing with a penetration enhancer, and adding into a temperature-sensitive hydrogel system to prepare the composition for promoting hair regeneration after chemotherapy. The composition for promoting hair regeneration after chemotherapy has a good transdermal absorption promoting effect, can slowly release drugs, promotes hair follicles to be converted from a resting stage to a growing stage under the synergistic effect of the drugs, regulates and controls multiple pathways (such as anti-apoptosis, angiogenesis promotion and anti-inflammation) at the same time, is matched with a complex pathological mechanism of hair regeneration after chemotherapy, and can promote hair regeneration after chemotherapy. Wide application prospects are realized.

The invention relates to the technical field of biological medicine, in particular to a wound repair material and a preparation method and application thereof.The wound repair material is in a gel shape and comprises hydrogel and platelet-rich plasma, the hydrogel is formed by dynamic crosslinking of collagen and oxidized hyaluronic acid, and the hydrogel physically encapsulates the platelet-rich plasma. The gelatinous wound repair material provides bionic mechanical support through a dynamic cross-linked network, synergistically regulates and controls an inflammation microenvironment and sequential release of PRP growth factors, remarkably promotes angiogenesis and wound healing, is suitable for the clinical treatment fields of diabetic ulcer, chronic difficult-to-heal wounds and the like, and has broad application prospects. The material has the functions of mechanical support, anti-inflammatory regulation and growth factor controlled release.

The invention provides an anti-aging repair composition containing PDRN, and lipid nanoparticles and application thereof. The composition provided by the invention is prepared from polydeoxyribonucleotide, micro-current tetrapeptide-1 and palmitoyl tripeptide-5. According to the application, the polydeoxyribonucleotide, the micro-current tetrapeptide-1 and the palmitoyl tripeptide-5 are compounded for use, so that the composition has a synergistic effect in the aspects of promoting cell proliferation, inhibiting an intracellular ROS (reactive oxygen species) level and the like, and has remarkable antioxidant, anti-inflammatory, anti-aging, soothing and repairing effects. The PDRN-containing composition is prepared into the lipid nanoparticles, so that the stability of the PDRN-containing composition is improved, the permeability of the PDRN-containing composition on the skin surface is improved, the bioavailability of the PDRN-containing composition is improved, the cell survival rate and cell uptake are promoted, the expression of adenosine A2A receptors, the synthesis of collagen, angiogenesis and the inhibition of anti-inflammatory factors are promoted, and a remarkable anti-aging effect is achieved.

The invention discloses photo-thermal controlled-release nitric oxide anti-biofilm hydrogel based on click reaction crosslinking as well as a preparation method and application of the photo-thermal controlled-release nitric oxide anti-biofilm hydrogel based on click reaction crosslinking, the hydrogel is prepared by taking azide group-containing four-arm polyethylene glycol and alkynyl modified hyaluronic acid as matrixes through click chemistry rapid crosslinking under the catalysis of copper-based nanoparticles, the antibacterial biological membrane has remarkable dual anti-biological membrane and anti-inflammatory effects, and can be applied to wound treatment of bacterial biological membrane infection; through a wound surface coating form, under near-infrared light irradiation, copper nanoparticles generate a mild photothermal effect to remove a biological membrane, and Cu < 2 + > is released to promote fibroblast proliferation; nitric oxide can be controllably released, a biological membrane is synergistically eliminated by enhancing lipid peroxidation and destroying a bacterial membrane structure, meanwhile, angiogenesis is promoted, collagen deposition is stimulated, and the wound healing process is accelerated; the invention has the functions of efficient antibiosis and tissue regeneration, and has wide clinical application prospect and development value in the field of infectious wound treatment.

Disclosed are methods, means, and compositions of matter useful for the stimulation of angiogenesis directly by administration of membrane vesicles, such as fibroblast-derived exosomes, and / or through induction of angiogenic cytokines from blood cells contacted with fibroblast-derived exosomes. The invention provides means of treating conditions in which angiogenesis is beneficial through local or systemic administration of exosomes, including those derived from fibroblasts, wherein the fibroblasts are cultured under basal conditions or conditions of hypoxia. In other embodiments exosomes derived from fibroblasts are utilized to augment endogenous regenerative processes, such as hematopoiesis, angiogenesis and neurogenesis, as well as augment regenerative processes stimulated by administration of exogenous therapeutics such as cells, growth factors, or genes.

The invention discloses a pharmaceutical composition for promoting angiogenesis and tissue regeneration and application thereof. The pharmaceutical composition of the present invention comprises an acidic secretory protein rich in cysteine, a disaccharide chain proteoglycan, and an XV-type collagen alpha1 chain. The biological material system loaded with the three types of proteins can promote angiogenesis, and can improve the tissue regeneration efficiency of bone, cartilage and skin defect areas.

A novel strategy for wound healing involving the coating of oxygen nanobubbles with exosomes and incorporating them into a polyvinyl alcohol (PVA) / gelatin hybrid hydrogel. In this work, we not only mitigate wound hypoxia but importantly have an efficient delivery method of exosomal nanoparticles, which to date has been confounded by very low to lack of uptake by cells in hypoxia. Furthermore, the self-healing properties of the hydrogel, along with its gelatin component, aids in hemostasis, thereby benefiting acute and surgical wound healing. Additionally, the crosslinking bonds within the hydrogel facilitate the decomposition of hydrogen peroxide (H2O2), alleviating wound inflammation. The multifunctional hydrogel provides for enhanced wound healing through increased angiogenesis, enhanced exosome delivery, hypoxia mitigation, and inflammation inhibition.

The invention discloses a composition for preventing hair loss and promoting hair growth as well as a preparation method and application thereof, and particularly belongs to the technical field of cosmetic raw material compounding. The anti-hair loss and hair growth composition is prepared from the following components in parts by mass: 12 to 16 parts of polygonum multiflorum root extract, 9 to 13 parts of ginger root extract, 0.7 to 1.3 parts of cymbidium hookerianum root extract and 1 to 2 parts of astragalus sinicus extract. According to the composition provided by the invention, the polygonum multiflorum root extract, the ginger root extract, the cymbidium hookerianum root extract and the astragalus sinicus extract have a multi-mechanism synergistic effect, so that the activity of 5 alpha-reductase is inhibited, damage of DHT to hair follicles is reduced, hair follicle angiogenesis is promoted, and hair follicle degeneration is delayed; the shampoo containing the anti-hair loss and hair growth composition provided by the invention has excellent anti-hair loss and hair growth effects.

The present invention relates to the use of lemon exosomes in the preparation of a drug for preventing, alleviating, or treating skin ulcers. The invention creatively extracts and purifies exosomes from lemons and studies their physiological effects. Lemon exosomes can reduce the expression of inflammatory factors in macrophages, promote the healing of skin ulcers, lower blood sugar, and promote skin angiogenesis. Lemon exosomes have the advantages of good efficacy, simple raw material acquisition, and minimal toxic and side effects.

The invention relates to bone paste for repairing bone wounds and a preparation method of the bone paste, and belongs to the technical field of biomedical material manufacturing. The bone paste is prepared from nanoscale calcium-phosphorus biphase ceramic, biomimetic mineralized collagen fiber, biodegradable polymer, modified hyaluronic acid, beta-cyclodextrin coated recombinant human bone morphogenetic protein-7, an angiogenesis accelerant and an immunoregulation additive according to a certain proportion. The nano-scale calcium-phosphorus dual-phase ceramic is hydroxyapatite and beta-tricalcium phosphate dual-phase ceramic; the biomimetic mineralized collagenous fiber is prepared by mixing type I collagen freeze-dried fiber with hydroxypropyl chitosan and performing mineralization; the modified hyaluronic acid is hyaluronic acid-sulfydryl and hyaluronic acid-hydrazide; the angiogenesis accelerant is a polylactic acid sustained release microsphere loaded with tanshinone and hydroxyl polyethylene glycol biotin; the immunoregulation additive is disodium glycyrrhizinate, lentinan and astragalus polysaccharide. The bone paste can effectively improve the bone induction capacity, promote vascularization generation and reduce the immunogenicity risk.

The composition is of a network structure and is composed of strontium ions and chlorogenic acid, and the weight ratio of the strontium ions to the chlorogenic acid is 1: (9-11). The composition and hydrogel are mixed to prepare a microneedle patch, and the microneedle patch is prepared into a medical instrument for wound repair. Through verification, the SrC-MPNs is continuously released by the SrC-MPNs (at) MN-PP, so that the substances are dissolved and absorbed into tissue fluid around a wound, and strontium ions and chlorogenic acid in the SrC-MPN can stably and continuously exert the effects of resisting bacteria and oxidation, promoting angiogenesis, promoting wound healing and the like. An in-vivo experiment result shows that the SrC-MPNs (at) MN-PP can promote in-vivo and in-vitro wound healing, promote M2 macrophage polarization, remove excessive ROS and promote angiogenesis and tissue regeneration so as to promote healing of diabetic wounds.

The invention relates to the technical field of composite hydrogel preparation, in particular to a nano composite hydrogel preparation process based on a microfluidic technology. The gel comprises the following components in parts by mass: 50-70 parts of a gel matrix, 5-9 parts of a nano enhancement system, 8-14 parts of a functional carrier, 3-6 parts of a dynamic response system, 4-7 parts of a hyaluronic acid-sulfhydrylated heparin compound and 0.5-1.5 parts of L-ascorbyl palmitate. Precise mixing and directional arrangement of all component materials on a micro-level are realized through a microfluidic technology, so that a nano reinforcing system and a functional carrier are uniformly distributed in a gel matrix, the synergistic effect of all the components is fully exerted, the mechanical property and the biological function of the gel are effectively improved, and the preparation method is suitable for industrial production. And quaternized chitosan nanofibers, a dynamic response system for responding to external environment change and a hyaluronic acid-sulfhydrylated heparin compound for promoting angiogenesis are introduced, so that the gel has the biological functions of resisting bacteria and promoting angiogenesis through a synergistic effect.

The invention is applicable to the technical field of hydrogel, and provides dynamic cross-linked hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: cross-linking oxidized dextran and carboxymethyl chitosan containing phenylboronic acid modification groups; glucanase is added before the hydrogel is formed, and calcium peroxide nanoparticles coated with polydopamine are introduced; wherein the mass concentration of the oxidized dextran is 2-10% w / v, the mass concentration of the carboxymethyl chitosan containing the phenylboronic acid modified group is 2% w / v, the concentration of the glucanase is 10-100 U, and the concentration of the calcium peroxide nanoparticles coated with the polydopamine is 0.1-1% w / v. The hydrogel disclosed by the invention shows good cell compatibility, angiogenesis promoting capability and bioactivity for promoting dental pulp regeneration in multiple in-vitro and in-vivo experiments, is suitable for tissue engineering fields such as dental pulp injury repair and the like, and effectively solves the technical problem that conventional hydrogel is insufficient in cell survival support in three-dimensional culture and the like.

The invention belongs to the technical field of medical gel, and particularly relates to modified hydrogel for skin wound healing and a preparation method thereof.The modified hydrogel is prepared from, by weight, 2-5 parts of drug-loaded GDVLNs, 1-2 parts of chitosan, 0.6-1.5 parts of antioxidant, 0.2 part of LAP, 1.5 parts of MA, 18 parts of gel matrix and 100 parts of buffer solution, the drug-loaded GDVLNs prepared from the traditional Chinese medicine active ingredients and the ginseng vesicles have the functions of resisting oxidative stress, promoting angiogenesis, promoting cell proliferation and the like, and the MA modified hydrogel system can realize slow release and controlled release of the drug-loaded GDVLNs and play a long-acting healing promoting role.

Methods for identifying a modulator of angiogenesis and methods for modulating angiogenesis in a mammalian subject are described herein. In some embodiments, the methods include obtaining data sets from a model for angiogenesis and generating a causal relationship network model based on the obtained data. In some embodiments, the methods include identifying, from the causal relationship network model, a causal relationship unique in angiogenesis, where a gene, lipid, protein, metabolite, transcript, or SNP associated with the unique causal relationship is identified as a modulator of angiogenesis.

The invention discloses a preparation method of antibacterial dual-oxygen-supply manganese-doped carbon dot-oxygen-carrying microbubbles, and belongs to the field of preparation of medical configuration products, and the preparation method comprises the following steps: attaching biotinylated manganese-doped carbon dots to oxygen-carrying microbubbles by using biotin-avidin connection to prepare micron-level manganese-doped carbon dot-oxygen-carrying microbubbles; the size of the micron-level manganese-doped carbon dot-oxygen-carrying microbubble is 1000-2500nm. The antibacterial dual-oxygen-supply manganese-doped carbon dot-oxygen-carrying microbubble prepared by the invention has the advantages of simple synthesis, clear structure, adjustable size and the like, and the material preparation method is simple, mild in reaction condition and suitable for large-scale production; manganese-doped carbon dot-oxygen-carrying microbubbles provide dual oxygen supply to enhance the sonodynamic therapy effect, so that efficient antibacterial activity is achieved, angiogenesis is promoted, wound healing is accelerated, and the method is a promising wound healing therapy method.

The invention discloses an adipose-derived stem cell exosome compound loaded with silver nanoparticles and application of the adipose-derived stem cell exosome compound loaded with the silver nanoparticles, and ADSC-Exos loaded with the silver nanoparticles is developed by integrating AgNPs into an exosome of ADSCs. In vivo, the Exo-AgNPs can accelerate healing of infected wounds by reducing bacterial colonization and promoting collagen deposition, angiogenesis and M2 type macrophage polarization. In vitro, the Exo-AgNPs activates the functions of fibroblasts and vascular endothelial cells, and shows an excellent antibacterial property. In addition, the Exo-AgNPs induces polarization of M2 type macrophages and inhibits secretion of proinflammatory cytokines by activating autophagy. The results show that Exo-AgNPs is expected to become an ideal biological material for treating infected wounds.

The invention belongs to the technical field of nano-drugs, and discloses a multifunctional nano-drug delivery system for treating neovascular eye diseases and a preparation method of the multifunctional nano-drug delivery system. The nano drug delivery system disclosed by the invention is a composite nano preparation which takes dendritic mesoporous silica nanoparticles as a carrier and co-loads cerium zirconium oxide nano enzyme and an anti-VEGF (vascular endothelial growth factor) drug, various active oxygen can be efficiently removed, oxygen is continuously released through catalytic decomposition of hydrogen peroxide, a retina hypoxia microenvironment is improved, and the retina hypoxia effect is improved. According to the present invention, the anti-VEGF drug delivery system can reduce the oxidative stress induced retinal pigment epithelial cell apoptosis rate, reduce the inflammatory reaction, and simultaneously achieve the slow release and the controlled release of the anti-VEGF drug, and the drug effect maintenance time can achieve more than 60 days, such that the multi-target synergistic treatment can be achieved through the synergistic regulation of the multiple pathological links such as oxygen deficit-oxidative stress-inflammation-angiogenesis, and the anti-VEGF drug delivery effect can be achieved. The choroidal neovascularization can be effectively inhibited by single intravitreal injection, and a new strategy of multi-target collaborative treatment is provided for neovascular macular degeneration and other eye diseases.

The invention discloses a magnetoelectric stent for realizing bone repair under external field driving and a preparation method and application thereof.The stent comprises a stent body based on a triple-period minimal curved surface structure and a magnetoelectric nano-particle coating, and the stent body comprises a convoluted and / or through hole structure; and the magnetoelectric nanoparticle coating is loaded on the inner surface of at least part of the pore structure. The magnetoelectric stent is suitable for providing better mechanical support for the early stage of bone repair of large-area bone defects, meanwhile, under the synergistic effect of magnetoelectric stimulation, osteogenesis and angiogenesis coupling can be promoted, and bone defect repair under the complex conditions of oral clinical alveolar bone vertical bone increment and systemic diseases such as inflammation and the like is achieved.

The present disclosure relates to novel compounds and pharmaceutical compositions thereof, and methods for promoting healthy aging of skin, the treatment of skin disorders, the treatment of cardiovascular disorders, the treatment of renal disorders, the treatment of angiogenesis disorders, such as cancer, treatment of tissue damage, such as non-cardiac tissue damage, the treatment of evolving myocardial infarction, the treatment of ischemic injury, and the treatment of various other disorders, such as complications arising from diabetes with the compounds and compositions of the invention. Other disorders can include, but are not limited to, atherosclerosis, coronary artery disease, diabetic nephropathy, diabetic neuropathy, diabetic retinopathy, diabetic cardiomyopathy, infections of the skin, peripheral vascular disease, stroke, asthma, and the like.

The present invention relates to a novel identified stem cell vMSC having angiogenesis characteristics, the vMSCs identified after being cultured under the culture conditions of the present invention are different from existing MSCs, are novel MSCs expressing CD141 cell surface antigens, have excellent cell expansibility, have an effect of directly forming blood vessels, have a paracrine effect of other MSCs, and are different from existing stem cells. The stem cell therapeutic agent for revascularization has a higher survival rate and a higher residual rate in tissues than conventional stem cell therapeutic agents, and can be used as a stem cell therapeutic agent for revascularization in which blood vessels are directly formed.

The invention relates to a myocardial tissue construct with a bionic structure and a function as well as a preparation method and application of the myocardial tissue construct. The myocardial tissue construct with both the bionic structure and the function comprises the following components: a hydrogel matrix suitable for 3D printing rheological properties, a cross-linking agent dispersed in the hydrogel matrix, inorganic biological material particles with myocardial protection and vascularization biological activity promotion, myocardial cells and vascularization related cells, the myocardial cells and the vascularization-related cells are regularly arranged in a three-dimensional space by simulating a cell distribution mode in a natural myocardial tissue; preferably, the regularly arranged structure comprises at least one of spatial arrangement of myocardial cell-angiogenesis related cells in a left-right / up-down side-by-side mode and spatial arrangement of myocardial cell-angiogenesis related cells in a core-shell / shell-core coaxial mode.

The invention provides a hydrogel dressing for synergistically promoting chronic wound healing through near-infrared controlled release of ROS (reactive oxygen species) and NO (nitric oxide) as well as a preparation method and application of the hydrogel dressing. Belongs to the technical field of biomedical materials. The hydrogel is prepared from the following components: upconversion nanoparticles as a core, silicon dioxide wrapping, carbon dot and L-arginine loading, maltotriose modification, and mixing and gelling with 2-amino-2-fluoro-2-deoxyadenosine as a hydrogel carrier, so as to obtain the hydrogel dressing. The obtained hydrogel dressing has the functions of inhibiting bacteria, promoting cell proliferation, promoting revascularization and the like, and remarkably promotes skin wound healing.

The invention relates to the technical field of biomedical materials, in particular to a microneedle patch for diabetes wound healing and a preparation method and application thereof. The microneedle patch comprises a substrate and a microneedle array arranged on the surface of the substrate, the substrate is prepared from a first raw material system comprising polyvinyl alcohol, phenylboronic acid modified oxidized hyaluronic acid, bromothymol blue and first drug micromolecules; the microneedle array is prepared from a second raw material system which comprises methylacryloylated gelatin, 2-(methylacryloyloxy) ethyl choline phosphate and platelet cell membrane wrapped MOF loaded second drug micromolecules. The microneedle patch has the characteristics of promoting angiogenesis, being excellent in antibacterial property and slow release, also has sensitive pH responsiveness and ROS responsiveness at the same time, can adjust the release dose of the medicine according to the wound surface microenvironment change (pH value change and ROS concentration change), can monitor the wound surface state in real time according to the wound surface pH value change indication, and is suitable for being popularized and used clinically.

The invention discloses application of a tumor-derived acellular extracellular matrix in preparation of a medicine for promoting angiogenesis and tissue regeneration. The invention provides a strategy for regulating and controlling tissue regeneration based on a tumor-derived acellular extracellular matrix microenvironment, a novel angiogenesis promoting biological material is constructed by combining the high similarity of a tumor microenvironment and a regeneration microenvironment such as angiogenesis, and high-efficiency regeneration is realized in various tissue defects. In addition, due to the characteristic of rapid proliferation of the tumor tissue, the productivity bottleneck of a traditional acellular extracellular matrix raw material source is broken through, and sustainable biological raw materials are provided for large-scale production. The method successfully solves the problem that a specific tissue-derived acellular extracellular matrix is difficult to regenerate in a complex damaged microenvironment.

The invention provides application of injectable hydrogel in preparation of a medicine for treating myocardial infarction. The injectable hydrogel is obtained by mixing aniline tetramer grafted gelatin, adipic dihydrazide modified hyaluronic acid and an aldehyde group-containing metal polyphenol complex. The injectable hydrogel has antioxidant activity, conductivity and angiogenesis promoting activity performance, and myocardial repair is promoted through synergistic regulation and control.

The invention provides application of alpha-ketobutyrate in preparation of drugs for prevention and / or treatment of skeletal muscle atrophy, and belongs to the technical field of biomedicine.The human endogenous metabolite alpha-ketobutyrate is applied to prevention and treatment of skeletal muscle atrophy, the side effect is small, the safety is high after long-term use, and the effect is obvious. Serious immunoreaction or toxic reaction can be avoided, and skeletal muscle senescence and muscular atrophy can be effectively prevented and treated. The alpha-ketobutyrate can improve the muscle index of skeletal muscles of the old mice, increase the number of muscle fibers, increase the cross sectional area, increase the number of muscle cell nucleuses and the expression of nuclear fiber layer protein LamB1 of the muscle cell nucleuses, and can also increase the capillary angiogenesis rate in muscle tissues of the old mice. Alpha-ketobutyrate has good pharmacological characteristics and low side effects, and provides an important basis for developing clinical drugs for treating amyotrophy.

The invention relates to the technical field of biological materials, in particular to composite hydrogel as well as a preparation method and application thereof. In particular to an ion response type ELPs-SA composite hydrogel constructed based on elastin-like polypeptides (ELPs) and sodium alginate (SA) as well as a preparation method and application of the ion response type ELPs-SA composite hydrogel. According to the hydrogel, elastic-like polypeptides ELP-Vs (such as adhesion promotion, angiogenesis, antibiosis and the like) with biological functions are introduced and react with SA, CaCO3 and gluconolactone (GDL) together, and the non-covalent cross-linked three-dimensional network hydrogel is formed under mild conditions. The obtained hydrogel has excellent mechanical strength (the energy storage modulus is 450-1773 Pa), a proper microporous structure (the pore diameter is 50-100 microns) and good biocompatibility, and can support three-dimensional culture of cells and promote angiogenesis and immunoregulation. The material has a wide application prospect in the biomedical fields of tissue engineering, wound repair, organoid construction and the like.