158 results about "Type I collagen" patented technology

The invention discloses a preparation method of a bionic cornea stroma lens for refractive correction, which comprises the following steps: solution preparation: dissolving type I collagen in an acetic acid solution, adding a film aid, fully stirring, and centrifugally defoaming to obtain a type I collagen composite solution; the I-type collagen composite solution is poured into an assembling groove, the curvature radius of titanium metal is set to be 6.8-8.0 mm, the titanium metal serves as a working electrode material, platinum metal serves as a counter electrode material, current parameters are set according to the needed thickness of the lens blank, the density is 3.50-6.50 mA / cm < 2 >, and the thickness of the lens blank is set to be 3.50-6.50 mA / cm < 2 >; a high-transparency cornea embryo material which is uniform in curvature in all directions and similar to human cornea fiber structure arrangement is formed after electrochemical assembly and cross-linking curing, accurate cutting machining is carried out through excimer laser, a concave / convex matrix lens with the needed correction degree is obtained, the correction precision is high after cornea interlayer implantation, and the correction precision is high. And meanwhile, the compound has excellent performance in the aspects of in-vivo stability and biological safety.

The invention provides a polyethylene glycol derivative-collagen cartilage repair gel which is characterized by being prepared from the following components in parts by weight: 8 to 12 parts of collagen (namely COL) solution, 0.5 to 2 parts of polydeoxyribonucleotide (namely PDRN), 4 to 8 parts of polyethylene glycol disuccinimide succinate (namely SS-PEG-SS) solution and 0.1 to 0.4 part of tannic acid (namely TA), and particularly discloses a preparation method and application of the polyethylene glycol derivative-collagen cartilage repair gel. In a word, the composite gel and the collagen are subjected to efficient amidation reaction through SS-PEG-SS to form a stable three-dimensional network, so that the structural support of the type I collagen, the tissue repair of PDRN and the anti-inflammatory and anti-oxidation characteristics of tannic acid are successfully integrated; therefore, the composite material has the comprehensive performance of good structural stability, high mechanical strength, good biocompatibility, controllable degradation period, excellent needle passing performance and the like, and shows the wide application prospects of clear repair effect, remarkable component synergistic effect, mature production process, outstanding cost effectiveness and the like.

The invention provides a recombinant I-type collagen with a thermostable triple helix structure and application thereof, and belongs to the technical field of protein engineering, the amino acid sequence of the recombinant I-type collagen is shown as SEQ ID NO: 1; the invention also provides a construction method of the expression system and a preparation method of the recombinant I-type collagen. The invention also provides practical industrial application according to the characteristics of the protein.

The invention belongs to the field of daily chemicals, and discloses a PDRN-containing composition with firming and anti-wrinkle effects, and the composition comprises PDRN, saururus chinensis extract, vetiver root oil, rusty rose seed oil and ginseng seed oil. According to the composition provided by the invention, the PDRN, the saururus chinensis extract, the vetiver root oil, the rusty rose seed oil and the ginseng seed oil are compounded for use, and the components are mutually matched and synergistically promoted to obtain a synergistic effect in the aspect of promoting the secretion of type I collagen, so that the composition has remarkable tightening and anti-wrinkle effects. In addition, the composition provided by the invention also has a certain barrier repairing effect, can significantly reduce the TEWL value of skin, is mild and non-irritant, and is suitable for people with sensitive skin.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention relates to a composition for preventing and improving skin aging and a cosmetic containing the same. The composition comprises a red rice extract and PDRN. The composition disclosed by the invention has a synergistic effect on promoting the generation of type I collagen, so that skin aging can be remarkably prevented and / or improved.

The invention discloses a composition containing stem cells and collagen and application of the composition in anti-aging products, and relates to the technical field of cosmetics, and the composition is prepared from 0.1-5 parts of umbilical cord mesenchymal stem cells, 1-20 parts of type I collagen, 3-15 parts of chitosan-hyaluronic acid microspheres, 1-10 parts of plant stem cell extract and 1-5 parts of exosome wrapped by liposome. Through component bionics, delivery system innovation and multi-target cooperation, the composition gives consideration to safety, effectiveness and use experience, and is expected to form differentiated competitiveness in a high-end anti-aging cosmetic market.

A method for synergistically promoting wound healing by means of moderate-intensity pulse ultrasonic waves and zinc oxide nanoparticle-type-I collagen hydrogel comprises the following steps that zinc oxide nanoparticles and type-I collagen hydrogel are physically mixed and crosslinked in advance and applied to a chronic infected wound, and then the wound is accelerated to heal through multiple times of MIPUS stimulation. The effect of the ZNP-COL hydrogel is enhanced through MIPUS, immune cell polarization can be activated, and the proportion of anti-inflammatory M2 type macrophages is increased; wherein ZNP is capable of releasing zinc ions and reactive oxygen substances and has antibacterial activity; meanwhile, ZNP can generate current and an electric field under stimulation of MIPUS, calcium ions on the surface of a cell membrane are activated to flow inwards, and proliferation of anti-inflammatory M2 type macrophages is promoted; the COL hydrogel is used as a medium to transmit ultrasonic waves and fix the ZNP, and the function of the ZNP at the local position is enhanced. The method solves the problems of bacterial infection and chronic inflammation at the same time, is safe, simple and reusable, and can be widely applied to infection of other parts besides the application.

The invention relates to the technical field of medical instruments, in particular to a variable-diameter blood seepage prevention artificial blood vessel based on a multi-layer composite structure, which comprises a composite artificial blood vessel main body, and a bioactive composite layer is arranged on the outer side of a gradient pore polyester woven layer. The main body of the composite artificial blood vessel is composed of more units such as the first artificial blood vessel part, the second artificial blood vessel part, the third artificial blood vessel part and the fourth artificial blood vessel part, and the inner diameter difference between every two adjacent units is 2.0 mm, so that the variable-diameter structure is more suitable for natural blood vessels of people clinically; in addition, the bioactive composite layer comprises type I collagen, heparin sodium and VEGF sustained release microspheres, the collagen / heparin composite layer forms a hydrated gel barrier when in contact with blood, the errhysis volume is reduced to 1 / 16 of that of a traditional product, endothelial cell migration is promoted by utilizing TiOnanotube array layer and VEGF sustained release synergy, and in addition, under the pulsating pressure of 120 mmHg, through 1 million times of cycle tests, the effective rate of the collagen / heparin composite layer is greatly increased. The structural integrity retention rate is larger than 99%, and the mechanical fatigue resistance is high.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for detecting the degradation capacity of human macrophages to liver collagen in vitro. According to the scheme provided by the invention, the content of CTX I, CTX III, C3M or C1M of the liver type I collagen degradation fragment in the macrophage supernatant is detected through Elisa, the degradation capability of the macrophage on the liver collagen can be accurately reflected, and the macrophage with the liver collagen degradation capability is qualified when the CTX I of the macrophage culture supernatant is greater than 100ng / mL.

The invention discloses a fully-protected polypeptide and a preparation method thereof, belongs to the technical field of polypeptide compound synthesis, and particularly relates to fully-protected peptide resin prepared through solid-phase synthesis. Carrying out cutting and unitary cyclization on the full-protection peptide resin to obtain unitary cyclic peptide; carrying out binary cyclization treatment on the unitary cyclic peptide to obtain a bicyclic peptide compound Cyclo (His-Cys-Lys-Gly-His-Cys-Lys-Gly-, and bridging a disulfide bond with Cysamp; the binary cyclization comprises cyclization under the action of iodo methanol and ascorbic acid; or the binary cyclization is cyclized in DMSO (Dimethylsulfoxide). The anti-aging and anti-oxidation bicyclic peptide compound prepared by the preparation method disclosed by the invention has the following beneficial effects of low toxicity, good stability, capability of improving I-type collagen expression, good anti-oxidation effect and good anti-inflammatory effect.

The invention relates to an application of Quercetin (QUE) or a derivative thereof in a medicine for resisting vocal cord fibrosis and a preparation of the Quercetin or the derivative thereof. The quercetin can significantly inhibit abnormal deposition of extracellular matrix (ECM) after vocal cord injury, improve reduction of hyaluronic acid (HA) and elastic fibers (EF), and reduce overexpression of type I collagen (COL1A1) and fibronectin (FN1). Besides, by regulating PI3K / AKT and MAPK signal channels, the quercetin inhibits proliferation and migration of fibroblasts and promotes apoptosis of the fibroblasts, so that vocal cord fibrosis is effectively relieved. The invention also provides an application of a preparation of the quercetin and the derivative thereof combined or not combined with other anti-fibrosis drugs (such as 5-fluorouracil and dexamethasone) in treatment of vocal cord fibrosis, wherein the dosage forms of the preparation comprise an oral preparation, an injection, a spray or a local gel preparation.

This invention relates to a gentle whitening and anti-aging composition for sensitive skin, its preparation method, and its application, belonging to the field of cosmetic technology. The gentle whitening and anti-aging composition includes hibiscus flower extract, Tokyo cherry blossom leaf extract, redwood seed extract, and vitamin C peptides. The Tokyo cherry blossom leaf extract is obtained using a eutectic solvent (DES) combined with enzymatic extraction and resin purification. This invention constructs a multi-level synergistic whitening and anti-aging network using the above components. Efficacy testing verifies that the components in the gentle whitening composition provided by this invention can synergistically enhance the composition's ability to inhibit tyrosinase activity, scavenge DPPH free radicals, and promote type I collagen synthesis, thereby achieving skin brightening and whitening, firming and anti-wrinkle effects, improved elasticity, and reduced fine lines.

This invention relates to an anti-wrinkle repair composition containing red camellia extract and its application. The anti-wrinkle repair composition of this invention comprises the following components in weight percentages: polyol 1: 1-25%; red camellia extract: 0.1-15%; creatine: 0.1-2%; carnosine: 0.01-0.5%; recombinant collagen: 0.01-5%; tetrahydrocurcumin inclusions: 1-20%; ergothioneine: 0.0001-1%; water: balance. This invention combats aging through multiple pathways and approaches, providing multi-dimensional skin anti-oxidation and anti-glycation, scavenging free radicals, promoting the production of type I collagen in the skin's extracellular matrix, and strengthening the skin barrier. This composition can be used as an active ingredient in various types of cosmetics, including aqueous solutions, lotions, creams, and shampoos.

This invention provides an organ fibrosis-related micropeptide, OFMP, and its applications. This type of micropeptide, OFMP, is a translational product with endogenous biological activity and stable existence, encoded by an open reading frame (ORF) in a long non-coding RNA. It is the first time that the long non-coding RNA encoding the micropeptide has been found to be significantly related to organ fibrosis and can encode the micropeptide. Synthetic peptides prepared based on the aforementioned OFMP micropeptide show significant inhibitory effects on several key indicators of organ fibrosis, including type I collagen α1 chain gene, α-smooth muscle actin, transforming growth factor β1, and connective tissue growth factor. This indicates that the OFMP micropeptide, the nucleotide sequence (ORF) encoding the micropeptide, and the long non-coding RNA sequence containing the nucleotide sequence have important clinical diagnostic and therapeutic value for organ fibrosis.

The invention belongs to the technical field of food preparation, and particularly relates to a biological information technology guidance-based compound bovine bone collagen peptide beverage, which is prepared from the following raw materials: bovine bone collagen peptide powder, red date polysaccharide, astragalus polysaccharide, calcium carbonate, chondroitin sulfate and sodium hyaluronate. When the compound bovine bone collagen peptide beverage is drunk, bovine bone contains a large amount of collagen, is rich in type I collagen and is richer in amino acid composition, and a large number of research results show that protein is not completely hydrolyzed into amino acid after being ingested, most of the protein is absorbed in a peptide form, and the protein is absorbed in a peptide form. Therefore, the selected bovine bone collagen peptide powder can be better absorbed by the human body. The red dates have the effects of maintaining beauty, keeping young and resisting aging. In addition, astragalus membranaceus and other traditional Chinese medicine extracts are supplemented in the compound bovine bone collagen peptide beverage, so that the functional characteristics of the beverage are enhanced, and the original bad flavor of animal peptide is improved.

The present application relates to the technical field of biomimetic materials, in particular to a multiphase biomimetic mineralized collagen scaffold, a preparation method and application thereof.The preparation method is as follows: type I collagen solution is self-assembled with solid copper sulfide nanoparticles, and through the technology of liquid phase mineralization, intracellular mineralization of collagen fibrils occurs, forming a multiphase biomimetic mineralized collagen scaffold similar to the nanostructure and Young's modulus of natural bone.The scaffold can improve the energy metabolism of cells by regulating the oxidative phosphorylation of bone marrow mesenchymal stem cells, thereby promoting the osteogenic differentiation of the cells; in addition, the scaffold releases copper ions to simulate enzyme activity, catalyzing the generation of a large amount of active oxygen in bacteria, leading to the rupture of the bacterial membrane and the overflow of the contents, thereby achieving the effect of antibacterial.The scaffold can treat simple bone defects or infected bone defects by regulating the energy metabolism of cells and simulating enzyme activity, thereby providing more new methods for the preparation of drugs for realizing bone regeneration and anti-infection in clinic.

This invention discloses a polypeptide with anti-wrinkle and oil-controlling effects, belonging to the field of cosmetic polypeptide technology. The polypeptide is heptapeptide-6, with the amino acid sequence Asn-Asp-Glu-Gly-Leu-Tyr-Leu. At concentrations of 0.001 mg / mL to 0.1 mg / mL, heptapeptide-6 significantly promotes the synthesis of type I collagen and elastin by human skin fibroblasts, exhibiting excellent anti-wrinkle and firming activity. At concentrations of 1 ppm to 50 ppm, it significantly inhibits lipid accumulation in *C. elegans* and lipid expression in zebrafish, with a maximum lipid inhibition rate of 24.38%, and also demonstrates clear oil-controlling activity. This invention's polypeptide has a single structure, overcoming the shortcomings of existing skincare polypeptides with single efficacy and complex formulations. It features stable processing, high safety, and is suitable as a core active ingredient in anti-wrinkle and oil-controlling cosmetics, providing a new avenue for the development and application of multifunctional skincare polypeptides.

The invention discloses a fully-protected polypeptide and a preparation method thereof, belongs to the field of polypeptide synthesis and application, and particularly relates to fully-protected peptide resin prepared through solid-phase synthesis. Carrying out cutting and monocyclization on the full-protection peptide resin to obtain monocyclic peptide; the monocyclic peptide is subjected to binary cyclization treatment to obtain a cyclic peptide compound Cyclo (His-Lys-Cys-Gly-His-Lys-Cys-Gly-, and a disulfide bond is in bridge connection with Cysamp; and the binary cyclization is cyclized under the action of iodine methanol and ascorbic acid. The cyclopeptide compound prepared by the invention has the advantages of low toxicity, good stability, good antioxidant effect and good anti-inflammatory effect, and the expression of type I collagen can be improved. Therefore, the invention has the advantages of low toxicity, good stability, good antioxidant effect and good anti-inflammatory effect, and can improve the expression of the type I collagen.

This invention discloses carboxymethyl sucrose amino acid salts, their preparation, compositions, and applications. The salts are prepared from carboxymethyl sucrose and arginine, or carboxymethyl sucrose and glycine, through a chemical reaction. The resulting salts undergo a series of processes including primary alcohol precipitation, redissolution, secondary alcohol precipitation, redissolution, tertiary alcohol precipitation, soaking, pulverization, filtration, and drying to obtain carboxymethyl sucrose arginine and carboxymethyl sucrose glycine salts. A composition is prepared by mixing these two salts. Both salts and the composition significantly inhibit the activity of matrix metalloproteinase 1 and promote the expression of type I collagen, thus delaying aging by maintaining the stability of collagen morphology and structure in the extracellular matrix. The preparation method of this invention is simple and the process conditions are mild. The product has the advantages of being weakly acidic, gentle on the skin, highly safe, and having good anti-aging effects. It can be widely used in skin care cosmetics and has promising application prospects.

The application belongs to the technical field of biomedical raw materials, and particularly relates to a Yanbian yellow cattle bone-derived type I collagen as well as a preparation method and application thereof. The preparation method comprises the following steps: crushing and layering and screening fat-removed Yanbian yellow cattle bones to obtain Yanbian yellow cattle bone particles, gradiently immersing the Yanbian yellow cattle bone particles in acid to obtain mineral-removed Yanbian yellow cattle bone particles, and then removing impure proteins; using pepsin for enzymolysis to obtain an enzymolysis liquid, and then performing salting-out to obtain a crude extract; and further purifying the obtained crude extract to obtain the Yanbian yellow cattle bone-derived type I collagen. The preparation method effectively removes inorganic salts (hydroxyapatite), fat and non-collagen protein impurities in bone tissues, and the content of the finally obtained type I collagen is greater than or equal to 800 mg / g, and the product has high purity.

The application relates to a recombinant type I collagen and application thereof, which utilizes the characteristics of Pichia pastoris capable of secreting and expressing recombinant proteins, secretes the recombinant type I collagen on the culture medium supernatant, and reduces the difficulty of protein purification. In addition, the integrin site RGD is inserted into the collagen to form a recombinant type I collagen with high stability and high expression. According to the relative proliferation of cells and the relative adhesion experiment of cells, it can be seen that the recombinant type I collagen has the effects of promoting the proliferation of skin fibroblasts, improving the skin, promoting wound healing, promoting the adhesion of fibroblasts in the skin layer and the like. Therefore, the recombinant type I collagen has great potential applications in the fields of skin care and beauty, medical treatment and the like.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

Provided are a high-stability recombinant type I collagen and a use thereof, relating to the technical field of collagens. By means of the directional selection of existing amino acid sequences, potential enzyme cutting sites and unstable sequence fragments are avoided during sequence design, enabling efficient expression in Pichia pastoris; and in addition, after being purified, the recombinant type I collagen still has high stability. The recombinant type I collagen takes into account factors such as related biological active sites and hydrophilicity, has related biological activity while maintaining high stability, and has good practicability.

The invention discloses application of cyclic peptide or salt thereof in cosmetics, and belongs to the technical field of cosmetic peptides, the natural cyclic peptide comprises alanine (Ala), glycine (Gly), proline (Pro), valine (Val) and tyrosine (Tyr), and is a compound with the following formula I. The invention further discloses a preparation method of the natural cyclic peptide. The cyclopeptide compound disclosed by the invention has the effects of promoting generation of type I collagen and elastin, inhibiting generation of matrix metalloproteinase I (MMP-1) under UVA irradiation and preventing collagen degradation, so that the cyclopeptide compound has the effects of resisting wrinkles, tightening skin and the like.

The invention discloses a preparation method of a bicyclic peptide compound, belongs to the technical field of polypeptide compound synthesis, and particularly relates to full-protection peptide resin prepared through solid-phase synthesis. Carrying out cutting and unitary cyclization on the full-protection peptide resin to obtain unitary cyclic peptide; carrying out binary cyclization treatment on the unitary cyclic peptide to obtain a bicyclic peptide compound Cyclo (His-Cys-Lys-Gly-His-Cys-Lys-Gly-, and bridging a disulfide bond with Cysamp; the binary cyclization comprises cyclization under the action of iodo methanol and ascorbic acid; or the binary cyclization is cyclized in DMSO (Dimethylsulfoxide). The anti-aging and anti-oxidation bicyclic peptide compound prepared by the preparation method disclosed by the invention has the following beneficial effects of low toxicity, good stability, capability of improving I-type collagen expression, good anti-oxidation effect and good anti-inflammatory effect.

The invention relates to the field of bone tissue engineering and regenerative medicine, in particular to non-denatured bone protein calcium superfine powder with bone tissue oriented growth and rapid repair effects and a preparation method and application of the non-denatured bone protein calcium superfine powder, and the non-denatured bone protein calcium superfine powder comprises the following components in percentage by weight: 25%-45% of non-denatured type I collagen, 0.005%-0.05% of bone morphogenetic protein-2, 0.001%-0.02% of transforming growth factor-beta1, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium The bone tissue repairing agent is prepared from, by weight, 0.001%-0.02% of basic fibroblast growth factors, 20%-40% of ultramicro hydroxyapatite nanoparticles, 5%-15% of chitosan oligosaccharide, 3%-10% of mannitol calcium complex, 2%-8% of sodium hyaluronate, 0.01%-0.1% of vitamin D3, 0.01%-0.1% of vitamin K2, 5%-15% of chondroitin sulfate, 1%-4% of casein phosphopeptides and the balance auxiliary materials, and directional growth and rapid repairing of bone tissue are promoted.

The invention relates to sesquiterpenoids Oxyphyllene F and Oxyphyllene G which are separated from fructus alpiniae oxyphyllae and application of the sesquiterpenoids Oxyphyllene G. Experimental research finds that the two compounds can inhibit the expression of fibronectin, type I collagen and alpha-smooth muscle actin in rat renal tubular duct epithelial cells induced by TGF-beta1, have anti-renal fibrosis activity, and can be used for preparing anti-renal fibrosis drugs. The fructus alpiniae oxyphyllae can be effectively used for preparing kidney protection drugs, the preparation method is good in reproducibility, the purity of the obtained compound is high, further pharmacological and clinical research on the compound is facilitated, the medicinal value of the fructus alpiniae oxyphyllae is expanded, and the economic and social benefits are huge.

The invention discloses application of gaboxadol or a pharmaceutically acceptable salt thereof in preparation of a medicine for treating liver fibrosis diseases, and belongs to the technical field of biological medicines, and research shows that the compound can influence collagen fiber crosslinking through targeted inhibition of the function of prolyl-4-hydroxylase a1 (prolyl-4-hydroxylase alpha 1, P4ha1), so that the anti-fibrosis effect is achieved. The invention further discloses an application of the gaboxadol or the pharmaceutically acceptable salt thereof in preparation of the medicine for treating the liver fibrosis diseases, and the application of the gaboxadol or the pharmaceutically acceptable salt thereof in preparation of the medicine for treating the liver fibrosis diseases and the application of the gaboxadol or the pharmaceutically acceptable salt thereof in preparation of the medicine for treating the liver fibrosis diseases. In a carbon tetrachloride induced mouse hepatic fibrosis model, the content of hydroxyproline and collagen deposition in I-type collagen molecules of the liver can be remarkably reduced by injecting the gaboxadol hydrochloride into the intraperitoneal cavity, and the hardness of the liver is reduced. A surface plasma resonance experiment proves that the probe has specific binding capacity with P4ha1. Toxicity experiments show that the compound is good in safety under the effective dosage. Therefore, the invention provides a new drug candidate and a theoretical basis for the treatment of hepatic fibrosis.

To provide a plant extract composition capable of significantly promoting secretion of type I collagen (COL-I) and elastin that are anti-aging proteins.SOLUTION: A plant extract composition is provided, the plant extract composition containing cherry blossom extract and prickly pear extract, and the solid component weight ratio of the cherry blossom extract to the prickly pear extract being 75:25-10:90. Preferably, the plant extract composition does not contain any other plant extracts.SELECTED DRAWING: None