302 results about "Type I collagen" patented technology

The invention discloses a recombinant XII type collagen as well as a preparation method and application thereof. The amino acid sequence of the recombinant XII type collagen is any one of (A1)-(A3); (A1) is an amino acid sequence as shown in SEQ ID NO: 1; (A2) is an amino acid sequence which is obtained by substitution, deletion or insertion of one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO: 1 and has the same functional protein; and (A3) is an amino acid sequence of a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of the sequence (A1) or (A2). The recombinant XII type collagen provided by the invention has good antioxidant activity, and has a repairing effect on the problems of skin laxity, wrinkle increase and the like caused by degradation of type I collagen fibers of aged skin.

The invention belongs to the field of biomedical materials, and particularly discloses a preparation method and application of a 3D printing porous composite scaffold. I-type collagen and silk fibroin composite gel is modified on a 3D printing three-element doped hydroxyapatite / polycaprolactone (PCL) composite scaffold with a polydopamine coating, the three-element doped hydroxyapatite is synthesized by using a chemical precipitation method, and Mg < 2 + >, Sr < 2 + > and SeO3 < 2-> ions are doped in crystal lattices. The 3D printing porous composite scaffold provided by the invention effectively simulates the structure and function of soft and hard combination of natural bones, is helpful for regulating and controlling the stem cell fate to improve the bone regeneration process, repairs bone defects while eliminating tumors, and can be applied to bone defect repair or osteosarcoma treatment.

The invention discloses a cyclic peptide compound as well as a preparation method and application thereof, belongs to the field of polypeptide synthesis and application, and particularly relates to full-protection peptide resin prepared by solid-phase synthesis. Carrying out cutting and monocyclization on the full-protection peptide resin to obtain monocyclic peptide; the monocyclic peptide is subjected to binary cyclization treatment to obtain a cyclic peptide compound Cyclo (His-Lys-Cys-Gly-His-Lys-Cys-Gly-, and a disulfide bond is in bridge connection with Cysamp; and the binary cyclization is cyclized under the action of iodine methanol and ascorbic acid. The cyclopeptide compound prepared by the invention has the advantages of low toxicity, good stability, good antioxidant effect and good anti-inflammatory effect, and the expression of type I collagen can be improved. Therefore, the invention has the advantages of low toxicity, good stability, good antioxidant effect and good anti-inflammatory effect, and can improve the expression of the type I collagen.

The invention discloses a preparation method of a bionic cornea stroma lens for refractive correction, which comprises the following steps: solution preparation: dissolving type I collagen in an acetic acid solution, adding a film aid, fully stirring, and centrifugally defoaming to obtain a type I collagen composite solution; the I-type collagen composite solution is poured into an assembling groove, the curvature radius of titanium metal is set to be 6.8-8.0 mm, the titanium metal serves as a working electrode material, platinum metal serves as a counter electrode material, current parameters are set according to the needed thickness of the lens blank, the density is 3.50-6.50 mA / cm < 2 >, and the thickness of the lens blank is set to be 3.50-6.50 mA / cm < 2 >; a high-transparency cornea embryo material which is uniform in curvature in all directions and similar to human cornea fiber structure arrangement is formed after electrochemical assembly and cross-linking curing, accurate cutting machining is carried out through excimer laser, a concave / convex matrix lens with the needed correction degree is obtained, the correction precision is high after cornea interlayer implantation, and the correction precision is high. And meanwhile, the compound has excellent performance in the aspects of in-vivo stability and biological safety.

The invention belongs to the technical field of functional foods and health foods, and particularly relates to an anti-saccharification and anti-aging aronia melanocarpa fruit composition and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 150 to 400 parts of aronia melanocarpa fruit fermentation liquor, 10 to 80 parts of lycium ruthenicum, 10 to 80 parts of ginseng, 0.5 to 2.5 parts of grape seed extract, 10 to 70 parts of collagen peptide, 0.5 to 4 parts of sodium hyaluronate, 0.1 to 0.8 part of resveratrol and 0.03 to 0.3 part of yeast extract. The content of type I collagen peptide in the skin is increased, the generation of in-vitro anti-glycation end products is inhibited, and the anti-glycation and anti-aging effects are achieved.

The invention provides a superfine-diameter PCL / COL nanofiber membrane as well as a preparation method and application thereof, and relates to the technical field of skin wound repair materials. The PCL / COL nanofiber membrane material is prepared from PCL and I-type collagen as raw materials through electrostatic spinning and vacuum drying. According to the preparation method disclosed by the invention, an electrostatic spinning technology is combined with strict regulation and control on parameters, so that the stable and superfine 40-nanoscale PCL / COL nanofiber membrane material is prepared. The diameter of the superfine PCL / COL nanofiber membrane material reaches the bionic scale of the minimum natural collagen of an extracellular matrix, and meanwhile, the superfine PCL / COL nanofiber membrane material shows more excellent hydrophilicity and lower surface roughness, has good biological safety and mechanical stability, not only realizes high matching with a natural ECM hierarchical structure, but also has good application prospects. By enhancing the cell-material interface interaction, the adhesion, proliferation and migration capacities of fibroblasts are remarkably improved, the wound healing period can be effectively shortened, an excellent repair promoting effect is shown, and a solid foundation is laid for clinical transformation of materials.

The invention discloses application of a siRNA composition in preparation of a medicine for treating liver diseases and the medicine. The siRNA composition at least comprises PCBP2-siRNA and PD-L1-siRNA. The invention discovers that PCBP2-siRNA and PD-L1-siRNA can effectively reduce type I collagen deposition in a mouse hepatic fibrosis model induced by CCL4 for the first time. The result shows that the two siRNAs both have a potential anti-fibrosis effect. And when the PCBP2-siRNA and the PD-L1-siRNA are used in a combined manner, the inhibition effect of the PCBP2-siRNA and the PD-L1-siRNA on the type I collagen deposition is more obvious than that of the PCBP2-siRNA which is independently used with any siRNA. The result shows that the liver fibrosis degree can be more effectively relieved through combined application of the two siRNAs.

The invention belongs to the technical field of biological medicine, and particularly discloses an artificial valve tissue calcification model and a preparation method and application thereof. The preparation method comprises the following steps: providing human valvular interstitial cells; the method comprises the following steps: providing a hydrogel system, wherein the hydrogel system comprises type I collagen, fibrinogen and Matrigel matrigel; constructing an engineered heart valve tissue strip by taking the hydrogel system as a skeleton structure between valve interstitial cells; and culturing the engineered heart valve tissue strip by using a calcification induction culture solution, so that the valve tissue generates a calcification phenotype. The model provided by the invention can generate a valve calcification phenotype which is similar to that after human heart valve calcification and is represented by valve interstitial cell activation, extracellular matrix remodeling such as collagen mass secretion and calcium deposition region generation. The model provided by the invention is low in cost, can be produced in batches, meets the research on the pathogenesis of extracorporeal valve calcification, and makes up for the dilemma that no artificial valve calcification model is available for drug research and development at present.

The invention belongs to the field of microorganisms, and particularly relates to application of lactobacillus paracasei L9 in preparation of a preparation for improving pulmonary fibrosis of an aged mouse. According to the lactobacillus paracasei L9 disclosed by the invention, the synthesis of I-type collagen is reduced by regulating and controlling gene expression, so that pulmonary fibrosis is relieved; meanwhile, the L9 improves the content of propionic acid and butyric acid in intestinal tracts and serum by regulating the abundance of SCFA-producing microbial flora in the intestinal tracts, so that pulmonary fibrosis is inhibited, and the L9 is a potential ideal preparation for improving or treating aging-related pulmonary fibrosis.

The invention relates to bone paste for repairing bone wounds and a preparation method of the bone paste, and belongs to the technical field of biomedical material manufacturing. The bone paste is prepared from nanoscale calcium-phosphorus biphase ceramic, biomimetic mineralized collagen fiber, biodegradable polymer, modified hyaluronic acid, beta-cyclodextrin coated recombinant human bone morphogenetic protein-7, an angiogenesis accelerant and an immunoregulation additive according to a certain proportion. The nano-scale calcium-phosphorus dual-phase ceramic is hydroxyapatite and beta-tricalcium phosphate dual-phase ceramic; the biomimetic mineralized collagenous fiber is prepared by mixing type I collagen freeze-dried fiber with hydroxypropyl chitosan and performing mineralization; the modified hyaluronic acid is hyaluronic acid-sulfydryl and hyaluronic acid-hydrazide; the angiogenesis accelerant is a polylactic acid sustained release microsphere loaded with tanshinone and hydroxyl polyethylene glycol biotin; the immunoregulation additive is disodium glycyrrhizinate, lentinan and astragalus polysaccharide. The bone paste can effectively improve the bone induction capacity, promote vascularization generation and reduce the immunogenicity risk.

The invention provides a small extracellular vesicle composite collagen hydrogel injection, and belongs to the technical field of osteoarthritis, and the small extracellular vesicle composite collagen hydrogel injection is prepared by the following steps: S1, mixing a sodium alginate solution with a type I collagen solution to obtain a mixed solution, adding a CaCl2 solution into the mixed solution to form gel, adding an FGF18 solution into the gel, and mixing to obtain FGF18 sodium alginate / collagen hydrogel; s2, transfecting a Lenti-IGF-1-EGF lentiviral vector to P3 generation human umbilical cord mesenchymal stem cells, culturing to obtain P5 generation IGF-1 overexpressed MSCs cells, namely P5 generation IGF-1-MSCs cells, co-culturing with the P5 generation IGF-1-MSCs cells by using curcumin, and separating and extracting a small extracellular vesicle solution rich in curcumin; and S3, mixing the small extracellular vesicle solution rich in curcumin with the FGF18 sodium alginate / collagen hydrogel, so as to obtain the small extracellular vesicle composite collagen hydrogel injection. The invention provides a small extracellular vesicle composite collagen hydrogel injection, which achieves the purposes of relieving inflammatory injury of osteoarthritis and enhancing the treatment effect of osteoarthritis.

The present invention belongs to the technical field of optimized coding gene, and discloses a recombinant human type I collagen, a preparation method thereof and an application thereof, and its amino acid sequence is shown as SEQ ID No.1. The recombinant type I collagen provided by the present invention has a high expression level, has better cell migration promotion, cell proliferation promotion and cell adhesion promotion properties, has high safety, and can be widely applied to fields such as medicine, medical devices, biological materials, tissue engineering, cosmetics and the like.

The invention discloses an animal source type I collagen liposome as well as a preparation method and application thereof. The method comprises the following steps: respectively dispersing two phospholipids and cholesterol in an organic solvent, carrying out rotary evaporation on the organic solvent, carrying out ultrasonic elution by using a buffer solution, homogenizing to form liposome balloons with uniform particle sizes, dialyzing by using the buffer solution, centrifuging to obtain liposome suspended matters, adding the suspended matters into an animal-derived I-type collagen solution with fixed concentration and pH, and carrying out freeze drying to obtain the collagen liposome. Stirring for a certain time at constant temperature, and centrifuging to obtain a final substance. The collagen coated with the lipidosome has a complete triple helix structure, and the D90 of the lipidosome is 680.51 nm. The liposome can be used for high-end cosmetics, such as essence, emulsion, cream, cream and other products.

The invention provides a polyethylene glycol derivative-collagen cartilage repair gel which is characterized by being prepared from the following components in parts by weight: 8 to 12 parts of collagen (namely COL) solution, 0.5 to 2 parts of polydeoxyribonucleotide (namely PDRN), 4 to 8 parts of polyethylene glycol disuccinimide succinate (namely SS-PEG-SS) solution and 0.1 to 0.4 part of tannic acid (namely TA), and particularly discloses a preparation method and application of the polyethylene glycol derivative-collagen cartilage repair gel. In a word, the composite gel and the collagen are subjected to efficient amidation reaction through SS-PEG-SS to form a stable three-dimensional network, so that the structural support of the type I collagen, the tissue repair of PDRN and the anti-inflammatory and anti-oxidation characteristics of tannic acid are successfully integrated; therefore, the composite material has the comprehensive performance of good structural stability, high mechanical strength, good biocompatibility, controllable degradation period, excellent needle passing performance and the like, and shows the wide application prospects of clear repair effect, remarkable component synergistic effect, mature production process, outstanding cost effectiveness and the like.

The invention discloses a recombinant I-type collagen, a preparation method and application, and belongs to the technical field of binding tissue peptides, the recombinant I-type collagen is formed by sequentially connecting the following modules in series from the N terminal to the C terminal: a signal peptide, a truncated I-type collagen and a His tag; the nucleotide sequence of the truncated I type collagen is as shown in any one of SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6; the nucleotide sequence of the truncated I type collagen is as shown in any one of SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6; according to the invention, a mammalian expression system and a 293T cell line are used for expressing recombinant I-type collagen, so that collagen with natural biological activity is obtained, and the proliferation efficiency and repair capacity of the collagen for promoting HSF cells can be improved.

The invention discloses a preparation method and application of a fish I-type collagen sodium alginate supramolecular assembly, fish processing byproducts are used as raw materials, and the fish I-type collagen is prepared by an acetic acid extraction and salting-out column chromatography purification integrated technology. The preparation method comprises the following steps: by taking fish type I collagen as a raw material, adding sodium alginate, and carrying out composite reaction, so as to obtain a mixed solution of the fish type I collagen, the sodium alginate and the fish type I collagen sodium alginate supramolecular assembly; and purifying the product by using a salting-out membrane separation integration technology to obtain the fish type I collagen sodium alginate supramolecular assembly. The fish type I collagen sodium alginate supramolecular assembly prepared by the invention keeps a triple helix structure of the type I collagen, shows good biocompatibility, and solves the application bottleneck problems of poor solubility and stability of the fish type I collagen and the like; the composition has the advantages of excellent solubility, high thermal stability, remarkable anti-wrinkle and moisturizing effects and good safety, so that the composition is very suitable for being applied to the field of anti-aging cosmetics.

The invention relates to an anti-aging composition and application thereof. The anti-aging composition is prepared from the following raw materials: dendrobium officinale, camellia japonica and polygonatum kingianum. Dendrobium officinale, camellia japonica and polygonatum kingianum cooperate with one another to achieve a synergistic effect, secretion of type I collagen (COL-1) can be promoted, secretion of matrix metalloproteinase-1 (MMP-1) can be inhibited, and decomposition of collagen in extracellular matrixes can be slowed down. The anti-aging composition can increase skin elasticity, improve the skin barrier function, reduce the percutaneous water loss rate and delay skin aging.

The present invention relates to a bone trauma repair bone paste and a preparation method thereof, belonging to the technical field of biomedical material manufacturing. The bone paste of the present invention is prepared by mixing nano calcium phosphate biphasic ceramics, biomimetic mineralized collagen fibers, biodegradable polymers, modified hyaluronic acid, β-cyclodextrin-coated recombinant human bone morphogenetic protein-7, angiogenesis promoters and immunomodulatory additives in a certain proportion; the nano calcium phosphate biphasic ceramics are hydroxyapatite and β-tricalcium phosphate biphasic ceramics; the biomimetic mineralized collagen fibers are prepared by mineralizing freeze-dried fibers of type I collagen admixed with hydroxypropyl chitosan; the modified hyaluronic acid is hyaluronic acid-thiol and hyaluronic acid-hydrazide; the angiogenesis promoter is a polylactic acid sustained-release microsphere loaded with tanshinone and hydroxy polyethylene glycol biotin; the immunomodulatory additive is disodium glycyrrhizinate, lentinan and astragalus polysaccharide. The bone paste can effectively improve the bone induction ability, promote angiogenesis and reduce the risk of immunogenicity.

The invention discloses application of a compound in preparation of a skin and / or scalp care product, the structural formula of the compound is # imgabs0, and compared with retinoic acid, the compound provided by the invention has higher cell safety concentration and lower irritation, has a more excellent effect in the aspect of cell damage repair, and has a better cell moisturizing effect; compared with the retinyl phytosphingosine, the compound provided by the invention has a better effect in the aspects of inhibiting the activity of elastase and improving type I collagen; compared with retinoyl phytosphingosine, the compound provided by the invention shows a better effect in the aspects of resisting lipid peroxidation and inhibiting malassezia reproduction, and also has better stability. The compound provided by the invention has good application value in the field of preparing cosmetics, medicines and health care products with corresponding effects or indications.

The invention provides a recombinant I-type collagen with a thermostable triple helix structure and application thereof, and belongs to the technical field of protein engineering, the amino acid sequence of the recombinant I-type collagen is shown as SEQ ID NO: 1; the invention also provides a construction method of the expression system and a preparation method of the recombinant I-type collagen. The invention also provides practical industrial application according to the characteristics of the protein.

The invention belongs to the field of biomedical materials, and particularly discloses a preparation method and application of a 3D printing porous composite scaffold. I-type collagen containing growth factors and silk fibroin composite gel are modified on a 3D printing three-element doped hydroxyapatite / polycaprolactone (PCL) composite scaffold with a polydopamine coating, the three-element doped hydroxyapatite is synthesized by using a chemical precipitation method, and Zn < 2 + >, Sr < 2 + > and SeO < 3-> ions are doped in crystal lattices. The 3D printing porous composite scaffold provided by the invention effectively simulates the structure and function of soft and hard combination of natural bones, is helpful for regulating and controlling the stem cell fate to improve the bone regeneration process, repairs bone defects while eliminating tumors, and can be applied to bone defect repair or osteosarcoma treatment.

The invention discloses an extracellular vesicle derived from rhodomonas mucilaginosa and a hydrogel composition of the extracellular vesicle. The extracellular vesicle is obtained by carrying out ultrafiltration concentration and gradient ultracentrifugation on a culture bacterium solution. Compared with an original culture solution or a viable bacterium preparation thereof, the treatment efficiency and the safety of the extracellular vesicles are remarkably improved. Based on the extracellular vesicle, the invention also provides a hydrogel composition loaded with the extracellular vesicle, and the hydrogel composition is constructed from the extracellular vesicle, type I collagen, carboxymethyl chitosan and four-arm polyethylene glycol benzaldehyde in a chemical crosslinking manner, and has good adhesiveness, self-repairing ability and injectability. The extracellular vesicle, the hydrogel composition and the hydrogel preparation of the extracellular vesicle can serve as a medicine or a medicine carrier to be applied to atopic dermatitis, can specifically target 2-type inflammatory factors, and relieve pathological changes such as skin lesion phenotype, epidermis thickening, mast cell infiltration and inflammatory factor expression increase of an MC903-induced atopic dermatitis mouse model.

The invention belongs to the field of daily chemicals, and discloses a PDRN-containing composition with firming and anti-wrinkle effects, and the composition comprises PDRN, saururus chinensis extract, vetiver root oil, rusty rose seed oil and ginseng seed oil. According to the composition provided by the invention, the PDRN, the saururus chinensis extract, the vetiver root oil, the rusty rose seed oil and the ginseng seed oil are compounded for use, and the components are mutually matched and synergistically promoted to obtain a synergistic effect in the aspect of promoting the secretion of type I collagen, so that the composition has remarkable tightening and anti-wrinkle effects. In addition, the composition provided by the invention also has a certain barrier repairing effect, can significantly reduce the TEWL value of skin, is mild and non-irritant, and is suitable for people with sensitive skin.

The invention provides a medicinal and edible composition for preventing and treating osteoporosis and a preparation method thereof. The medicinal and edible composition comprises the following raw materials in parts by weight: 15-24 parts of prepared rehmannia root, 12-15 parts of dogwood, 12-20 parts of Chinese yam, 15-20 parts of astragalus membranaceus, 3-10 parts of angelica sinensis and 10-20 parts of folium cortex eucommiae; the medicinal and edible composition provided by the invention promotes osteoblast differentiation, inhibits osteoclast absorption, regulates bone metabolism balance, increases the maximum load of thighbone, and improves bone density and bone strength by increasing osteoprotegerin and reducing type I collagen C-terminal peptide and tartrate-resistant acid phosphatase. The medicinal and edible composition disclosed by the invention is high in safety, can be developed into medicines, health care products or functional foods, and is applied to prevention and treatment of osteoporosis.

The invention discloses biological glue and a preparation method thereof.The preparation method comprises the steps that gel and activation liquid are prepared, and the gel is prepared by evenly mixing I-type collagen freeze-dried powder, chitosan and a fibrinogen solution; and mixing the freeze-dried thrombin with a calcium chloride solution to prepare an activation solution. The meninx suturing device has the advantages that sliding in the meninx suturing process is effectively reduced, an operation is easier to carry out, the risk that the meninx or a repairing material slides after the operation can be reduced, and a wound is evenly healed; most of the components are type I collagen which is beneficial to meninx injury repair, and the components contain chitosan which has antibacterial and hemostatic effects.

The invention relates to glassine ester as well as a preparation method and application thereof. The structural formula of the glassine ester is shown as a formula (I), each R1 is independently selected from hydrogen and-C (= O) R, and at least one R1 is-C (= O) R; r is selected from C7-C21 alkyl groups and C7-C21 unsaturated chain alkyl groups. The glassine ester has a remarkable DPPH free radical scavenging effect, can remarkably improve expression of elastin, hyaluronic acid and I-type collagen in cells, and has excellent effects of oxidation resistance, moisturizing and / or tightening and wrinkle resistance when being added into cosmetics as a functional component. # imgabs0 #

The invention discloses an anti-aging composition and application thereof. According to the anti-aging composition disclosed by the invention, the anti-aging composition is prepared by compounding dimethyl silanol hyaluronate, radix pseudostellariae cyclic peptide B and a medicago sativa extract. The medicago sativa extract disclosed by the invention is obtained by adopting a pulsed ultrasound-assisted subcritical extraction technology, and can play a better anti-aging effect. Experiments verify that the composition can significantly increase the content of retinoic acid binding receptor RAR alpha and RXR gamma proteins and type I collagen, inhibit cell aging and achieve an anti-aging effect similar to that of retinol, but compared with retinol, the composition is mild and non-irritant, can significantly improve the preservative efficacy of a product when being used in skin care products, and can reduce the dosage of preservatives.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention relates to a composition for preventing and improving skin aging and a cosmetic containing the same. The composition comprises a red rice extract and PDRN. The composition disclosed by the invention has a synergistic effect on promoting the generation of type I collagen, so that skin aging can be remarkably prevented and / or improved.

The present invention is concerned with a manufactured article prepared from a biomaterial comprising isolated and purified type I collagen fibrils derived from abalone and a method of preparing such an article. The manufactured article can be prepared by 3D bioprinting, and therefore the present invention also envisages a novel bioink for a 3D bioprinter comprising an isolated and purified type I collagen fibril and cartridges containing such a bioink. The invention additionally relates to a method of isolating type I collagen fibrils from abalone. The manufactured article is useful in a number of biomedical applications including as an implantable device where it can function as a 3D bioprinted hard or soft tissue scaffold for tissue engineering.