878results about "Surgical veterinary" patented technology

The invention relates to an animal craniocerebral injury model construction method and system, and the method comprises the following steps: 1, selecting a plurality of healthy male SD (Sprague Dawley) rats, breeding the rats in different cages in a 12-hour illumination environment and a 6-hour dark environment at the temperature of 25 DEG C, dividing the rats into three groups, and enabling the rats to eat and drink water; 2, the rats are divided into an X group, a Y group and a Z group through random grouping, the X group is a mild craniocerebral injury group, the Y group is a moderate craniocerebral injury group, the Z group is a severe craniocerebral injury group, and the number of the rats in each group is equal; according to the animal craniocerebral injury model, animal craniocerebral injury of different degrees can be simulated, the injury degree can be evaluated, and the model has good repeatability, reliability and stability and has good reference value in practical application.

The invention relates to the technical field of extracranial strike testing, in particular to digitally-controlled extracranial strike construction equipment of a traumatic brain injury model. Comprising a test platform, a sliding rail installed on the test platform and arranged in the length direction of the test platform, an animal fixing platform slidably connected with the test platform through the sliding rail, an impact structure arranged on one side of the test platform and a driving structure used for driving the animal fixing platform to move towards the impact structure. The impact structure is fixedly connected to the test platform, the output end of the impact structure faces the animal fixing platform, an experimental animal is installed on the animal fixing platform, the driving structure drives the animal fixing platform to enable the experimental animal to actively impact, and the impact structure enables the experimental animal to impact a fixed acting point at a certain initial speed. The system is more in line with the injury mechanism of a patient with clinical actual craniocerebral trauma, and a tester can accurately control and monitor parameters according to the moving speed and the moving distance of the animal fixing platform.

The invention provides a tumor living body sampling device, and relates to the field of biological sampling, the tumor living body sampling device comprises: a sampler main body, the sampler main body is a pistol-shaped structure; the inner sampling needle is coaxially and rotatably connected to the inner side of the reversing seat, the inner sampling needle is rotatably connected to the inner side of the sampling outer cylinder, the right cross sections of the sampling outer cylinder and the inner sampling needle are of arch-shaped structures, and the right end parts of the sampling outer cylinder and the inner sampling needle are of needle-shaped spine structures; hollow sampling openings are formed in arch-shaped parts on the right sides of the sampling outer cylinder and the inner sampling needle; the sampling transmission assembly is arranged in the sampler main body, and the sampling operation block drives the sampling outer cylinder and the inner sampling needle to rotate reversely at the same time through the sampling transmission assembly. The tumor tissue is rapidly and completely cut off, tearing damage is avoided, samples are isolated from the tissue, the samples are prevented from being mixed, and the problems that in the existing sampling process, the tissue is difficult to effectively cut off, tissue tearing is easily caused, and tissue samples are not pure are solved.

Provided is a technology which can properly perform a predetermined treatment on an abdomen of a test fish while reliably holding the test fish in a predetermined posture by suppressing damage as much as possible. A test fish holding device includes a flexible holder, the holder having a holding groove extending along a front-rear direction on an upper surface side and internally holding the test fish loaded from an upper opening portion. The holder has a slit extending along the front-rear direction in a bottom portion of the holding groove and exposing the abdomen of the test fish held inside the holding groove through a lower surface side. A width of the slit is widened by generating a tensile force from an inside toward an outside on the lower surface side of the holder.

The invention relates to a segmented cutting device and system for a rumen fistula. The segmented cutting device comprises a first handle unit, a second handle unit, a first baffle unit, a second baffle unit, a first cutting processing unit and a second cutting processing unit. The device has the advantages that a stable holding point is provided for an operator through cooperative use of the first handle unit and the second handle unit, the whole device is convenient to control, accurate operation is facilitated, misoperation caused by unstable holding is avoided, and the risk of accidentally injuring dairy cows is reduced; the first baffle unit and the second baffle unit are used in cooperation and can abut against the fistula inner wall and the fistula outer wall, a cutting area can be isolated from surrounding dairy cow tissue, the protection effect is achieved, contact between a cutter and other tissue is reduced, the risk of injury to other parts of the dairy cow body is reduced, the cutting position is accurately limited, and the cutting efficiency is improved. The cutter is prevented from deviating from the preset position in the cutting process.

The invention discloses an intelligent control method and system for irreversible electroporation regeneration of cartilage tissue. The method comprises the following steps: selecting an experimental animal to establish an osteoarthritis model, and carrying out quantitative analysis on the osteoarthritis model through an algorithm; designing and preparing a specific gene vector; performing an electroporation process to introduce the specific gene vector into the target cartilage tissue; monitoring gene transfection efficiency and expression level in real time, and dynamically adjusting electroporation parameters according to real-time monitoring data; the cartilage regeneration and function recovery conditions are evaluated through a multi-modal imaging and histological method; and performing comprehensive analysis based on an evaluation result, and dynamically adjusting an electroporation parameter and a gene transfection strategy. The system comprises an osteoarthritis model establishment module, a carrier design module, a carrier import module, a dynamic adjustment module, an image evaluation module and a comprehensive analysis module. According to the method, quantitative evaluation and dynamic feedback adjustment of the cartilage repair effect are realized through an algorithm and multi-modal data analysis.

The invention belongs to the technical field of biological electrodes, and particularly relates to a flexible multi-channel implantable biological electrode, an implanting device and a method, and the biological electrode is formed by fixing at least two single-channel electrodes into a bundle in a spiral winding or parallel fixing mode; adjacent single-channel electrodes abut against and are fixed and insulated from each other, the single-channel electrodes are respectively provided with exposure sites used for collecting electroneurographic signals and / or electromyographic signals or used for nerve electrical stimulation and / or electromyographic stimulation at the implantation sections, and the single-channel electrodes have determined relative positions and jointly form multiple channels; and the exposed sites are arranged at the end part and / or the side surface of the implantation section of the single-channel electrode. Compared with the prior art, the problem that each single-channel electrode in a'multi-channel 'biological electrode in the prior art does not have certain relevance is solved. According to the scheme, all the single-channel electrodes forming the flexible multi-channel biological electrode have a clear relative position relation, and electrode exposure point positions can be arranged at designated positions according to needs.

The invention relates to the technical field of biology and new medicine, in particular to a preparation method, an identification method and a histopathologic analysis method of a rat uterus scar diverticulum model. The preparation method of the rat uterus scar diverticulum model comprises the following steps: sequentially simulating rat pregnancy by using female progestational hormone, and infecting a uterus incision by using group B streptococcus (GBS) to obtain the rat uterus scar diverticulum model. The preparation method comprises the following steps: constructing a pseudopregnant rat model; preparing a GBS bacterial solution with a certain concentration, and immersing 3-0 silk threads with the length of about 1cm into the bacterial solution for 24 hours for later use; an incision with the length of about 2 cm is formed in the uterus on the two sides of the rat, and the incision is sutured discontinuously by an absorbable line. The simulated pregnant mouse is used, the influence of uterine hyperemia during human delivery and hormone change after female progestational hormone withdrawal on the uterine microenvironment is met, the formation of the human uterine scar diverticulum simulated by using the group B streptococcus infection incision is related to cesarean delivery incision infection, and therefore the success rate of the obtained model is high.

The present invention is an apparatus and method for ligation of livestock animals. The ligation apparatus or band provides topical active agent(s) that alleviate pain during various ligature procedures. This invention also relates to a method that includes applying a loop or band around a body part for elastomeric ligation, whereby the band material supplies a topical active agent (s) to the body part to reduce the pain of ligation.

The invention discloses a rat caudal vertebra intervertebral disc degeneration molding device which comprises an anesthesia box, a tail outlet hole is formed in the anesthesia box, a bearing table is arranged on the outer side of the anesthesia box, an arc-shaped locking plate is slidably installed on the bearing table, a containing groove is formed between the arc-shaped locking plate and the bearing table, and a sleeve is slidably installed on the arc-shaped locking plate in the circumferential direction; a puncture cylinder is in threaded connection with the interior of the sleeve, a graduated scale is axially arranged on the outer side wall of the puncture cylinder, a puncture needle is installed in the puncture cylinder and comprises a base and a needle head connected to the base, the base is installed in the puncture cylinder, the needle head sequentially penetrates through the puncture cylinder, the sleeve and the arc-shaped locking plate and then extends into the containing groove, and a bandage is arranged above the bearing table. An X-ray machine is arranged on the periphery of the bearing plate. The invention further discloses a rat caudal vertebra intervertebral disc degeneration modeling method using the rat caudal vertebra intervertebral disc degeneration modeling device. According to the rat caudal vertebra intervertebral disc degeneration modeling device and method, few tools are used, the process is simple, and application and popularization are convenient.

The utility model discloses a rat thrombus forming device which comprises a blood collecting assembly and a thrombus forming assembly, the blood collecting assembly is used for collecting blood of a rat, and the blood collected by the blood collecting assembly is conveyed to the thrombus forming assembly to prepare thrombus; the blood sampling assembly comprises a bottom plate and a top plate, the top plate is located over the bottom plate, a vertical supporting rod is connected between the center of the bottom plate and the center of the top plate, the top plate can rotate around the supporting rod, a plurality of round holes are formed in the top plate in the annular direction, and blood collecting pipes are vertically placed in the round holes; a liquid outlet is formed in the bottom of the blood collecting pipe, a blood transfer pipe is connected to the liquid outlet, and the head end of the blood transfer pipe extends to the thrombus forming assembly; the thrombus forming assembly comprises a rectangular forming groove, a forming template made of rubber is fixed to the bottom of the forming groove, pits with honeycomb holes are densely distributed in the forming template, the diameter of each pit is 2-3 mm, and the depth of each pit is 2-3 mm.

The utility model discloses a pair of adjustable nerve bruise forceps with pressure sensing function, which comprises a first forceps body, a second forceps body and a bruise clip, one end of the forceps body far away from a user is provided with a buckle, the buckle is connected with the bruise clip, and the first forceps body and the second forceps body drive the bruise clip to close after applying external force so as to bruise nerves. The bruise clamp is provided with various types of adaptive buckles, the bruise width of the nerve bruise clamp is adjusted to adapt to different experiment requirements and requirements of experiment objects, a pressure sensor is arranged at the position, close to the buckles, of the clamp body, the pressure sensor sends a sensing signal to a controller, an operator can control own force through a display screen of the sensor, and the operation is convenient. And the nerve injury degree is kept consistent. The dependence of the crushing degree on an operator is reduced, the operation fatigue is relieved, and a more accurate, scientific and efficient medical instrument is provided for animal experiment modeling of nerve crushing injuries.

The invention discloses an adjustable spinal cord impact vibration injury device and an injury method thereof, and belongs to the technical field of spinal cord injury experiment research. Two lifting sliding rods are fixedly installed at the rear end of the base, and lifting sliding blocks capable of vertically sliding are installed on the lifting sliding rods. An angle adjusting mechanism is installed on the lifting sliding block, the movable end of the angle adjusting mechanism is detachably connected with a supporting tool used for fixing the experimental animal, and the angle adjusting mechanism is used for adjusting the angle of the supporting tool so that different parts of the experimental animal can face the base; the base is further provided with an impact triggering mechanism. Through the mode, the experimental animal spinal cord impact oscillation injury model prepared by adopting the technical scheme can well simulate the injury process of a clinical spinal cord injury patient and the pathophysiological change after injury; and the spinal cord impact vibration injury models with different injury modes and different injury degrees are prepared by changing the free falling body height of the experimental animal, the vertical impact rods made of different materials and the impact point location of the experimental animal.

The invention discloses a preparation method and system of a mouse craniocerebral thoracico-abdominal combined injury dynamic model based on AI, and belongs to the technical field of biomechanical injury. The method comprises the steps that impact is applied to a mouse skull, and the impact is conducted along an organ synergistic conduction chain so as to induce the craniocerebral thoracico-abdominal combined injury of the mouse; in the impact applying process, multi-modal physiological data are synchronously collected; and inputting the collected multi-modal physiological data into an AI analysis model, analyzing the injury mechanism of the craniocerebral thoracic-abdominal combined injury, and establishing a dynamic model based on an analysis result. According to the technical scheme provided by the invention, high-simulation modeling of multi-organ cascade injury is realized by constructing the organ synergistic conduction chain from the brain to the thoracic cavity; in combination with multi-modal physiological data synchronous acquisition and an AI analysis model, injury time sequence characteristics can be accurately extracted, a causal path of a local injury initiation system response is established, and the precision and practicability of compound injury mechanism prediction are improved.

The invention relates to the technical field of medical experimental instruments, and discloses a stereotactic experimental mouse cerebrospinal fluid automatic extraction device which comprises a supporting seat, a supporting frame is fixedly connected to the top of the supporting seat, a first air cylinder is fixedly connected to the outer wall of the supporting frame, and the output end of the first air cylinder penetrates through the outer wall of the supporting frame and is fixedly connected with an extraction pipe. A second air cylinder is fixedly connected to the inner wall of the supporting seat, a first sliding groove is formed in the outer wall of the supporting seat, an adjusting mechanism is arranged in the supporting seat, the positioning mechanism is arranged in the supporting seat and comprises a positioning block, and a first supporting rod is fixedly connected to the outer wall of the positioning block. The mouse is positioned through the positioning mechanism, and the head of the mouse is clamped, so that the head of the mouse is fixed, the stability of the cerebrospinal fluid extraction process is improved, the operation difficulty is reduced, and the extraction device can extract the cerebrospinal fluid of the mouse more stably.

The invention provides a construction method and application of an osteoarthritis animal model. According to the construction method, a standardized experimental Beagle dog is selected, an improved Hulth method is used for establishing the osteoarthritis model, a mode of inducing the experimental dog to move is optionally adopted for further treatment, and the animal model close to the clinical actual situation is constructed. The animal model obtained through the construction method fits traumatic arthritis caused by increasingly worn joints in actual life, the model stability is good, and a new animal model with important practical value is provided for research and development of osteoarthritis drugs and medical instruments.

According to the organ tissue sampler for the frozen dead pigs, the head of an electric drill is fixedly connected with a drill bit seat, a drill rod connected with a motor is arranged on the drill bit seat, one of a first sawtooth disc and a second sawtooth disc is selected to be matched with the drill rod, and the outer diameter of the first sawtooth disc is larger than that of the second sawtooth disc; the center of the second sawtooth disc is sleeved with a mounting sleeve, and one end of the mounting sleeve is detachably connected with the drill rod and serves as a mounting end. An opening of the sample storage cavity is located in the front face of the second sawtooth disc, and a sampling tongue is connected to the sampling end. Through cooperation of an electric drill and a first sawtooth disc, the external skin of the frozen pigs died of illness is rapidly cut, so that internal tissue visceral organs are exposed; through cooperation of an electric drill and a second sawtooth disc, internal tissues and visceral organs are accurately sampled, through cooperation of a sampling tongue and a sample storage cavity, samples are conveniently collected, and the samples are loaded into collection consumables such as a centrifugal tube or a sampling bottle.

The invention discloses a method for establishing a cerebral ischemia reperfusion injury model, and relates to the technical field of biomedicine. Comprising the following steps: S1, preparing experimental materials, and selecting healthy adult male SD rats; s2, anesthesia and fixation: placing the to-be-treated SD rat in a closed container containing 7% isoflurane until the to-be-treated SD rat is unconscious, then transferring the to-be-treated SD rat to an operating table, continuing to give isoflurane with the concentration of 2%-3% to maintain a deep anesthesia state, and keeping the body temperature by adopting a constant-temperature blanket; s3, surgical operation: cutting a 1cm long mouth of the skin along the median line of the neck, and carefully separating common carotid artery and vagus nerve on two sides to avoid damage to surrounding tissues; the left side external jugular vein telecentric end is ligated with a silk thread to reduce the amount of bleeding. According to the scheme, the establishment process of the cerebral ischemia reperfusion injury model is described in detail, the establishment process comprises the steps of experimental material preparation, anesthesia and fixation, surgical operation, ischemia induction, reperfusion, nursing, data analysis and the like, and complete reference is provided for related research.

The utility model discloses a continuous injection device for a biomarker chip. Comprising an injector shell, a needle arranged at one end of the injector shell, a chip push rod arranged in the injector shell in a limited mode, a main push rod inserted into the injector shell in a matched mode and connected with the chip push rod, and a chip push rod spring arranged on the chip push rod in a sleeving mode. A chip clip is arranged on the injector shell and close to one end of the needle head, a chip boosting plate is arranged in the chip clip in a limiting manner, and a chip spring is arranged below the chip boosting plate; and a plurality of biomarker chips are carried on the top of the chip boosting plate. According to the utility model, through transformation and upgrading on the basis of the prior art, continuous operation of biomarker chip injection is realized, and the injection efficiency is improved; and the shell and the internal structure are further optimized, so that the device is more efficient and convenient.

The invention belongs to the technical field of medical instruments, and discloses a surgical instrument for building a traumatic brain injury mouse model.The surgical instrument comprises a tube blade, a limiter, a holding piece and a vacuum device, the tube blade is used for rotationally cutting into brain tissue of a mouse, the limiter is movably installed on the tube blade in the axial direction of the tube blade, and the vacuum device is arranged on the holding piece; the limiting device is used for abutting against the skull of the mouse, the holding piece is provided with an airflow channel, one end of the holding piece is connected with the pipe blade, one end of the airflow channel is communicated with the pipe blade, and the vacuum device is connected to the other end of the holding piece and connected with the other end of the airflow channel. According to the surgical instrument for building the traumatic brain injury mouse model, the problems that an injury area is uncontrollable and a focus area is difficult to quantify and unify in the current traumatic brain injury mouse model building process can be solved, so that the success rate and efficiency of model building are improved to the maximum extent.

The invention discloses a myocardial ischemia reperfusion injury animal model construction method and application thereof in the technical field of model animal preparation. The method comprises the steps of hypertension and hyperlipidemia pretreatment, standardized ischemia-reperfusion operation, immune microenvironment regulation and control and model evaluation and verification. According to the invention, model construction is carried out on an integrated model operation table, so that a standardized ischemia-reperfusion process is realized, reproducibility of a myocardial injury degree and an immune cell infiltration rule of the model is realized, and suitability of the model and nano-drug research is enhanced; and a reliable tool is provided for research and development of targeted drugs such as immune cell membrane coated nanoparticles.

The invention discloses an isolator for establishing a bone nonunion animal model and a preparation method of the isolator, and relates to the technical field of medical instruments, and the preparation method comprises the following steps: step 1, obtaining a hydrogen-containing silicone oil prepolymer with a side chain containing Si-H bonds; 2, obtaining a silica gel macromonomer with an epoxy group on a side chain; step 3, obtaining an anti-inflammatory functionalized silica gel macromonomer; 4, reconstructing the skeleton model in a three-dimensional manner, and creating a defect isolator model; 5, adding the anti-inflammatory functionalized silica gel macromonomer obtained in the step 3 and a reactive diluent into a mixing tank, adding a cross-linking agent and polyether modified polydimethylsiloxane under mechanical stirring, stirring for 30 minutes, defoaming for 2 hours under 500Pa vacuum, and standing and curing in a darkroom after defoaming; and 6, a multi-material-box DLP photocuring 3D printer is adopted for isolator printing. According to the method, observation interference on the bone nonunion pathological process is fundamentally reduced, and high-fidelity modeling construction is achieved.

The invention provides a method for establishing a mouse postoperative delirium model under a simulated high altitude urgent entry environment, and relates to the technical field of animal model establishment. According to the method for establishing the model for simulating postoperative delirium of the mouse in the sudden entering plateau environment, a control group (group C), a plateau group (group H), a POD model group (group P) and a plateau POD model group (group HP) are included, behavior changes of the mouse are evaluated through a Y maze experiment, then the mouse is killed, brain tissue is taken, and the mouse is subjected to postoperation delirium simulation in the sudden entering plateau environment. The mouse hippocampus tissue structure damage change is observed through morphological methods such as Nissl staining and a transmission electron microscope, meanwhile, blood is taken through mouse eye sockets, the concentration of inflammatory related indexes IL-6 and TNF-alpha in serum is measured through an ELISA method, and the stability and repeatability of model establishment are known. The plateau postoperative delirium mouse model prepared by the invention can generate obvious damage to brain tissues and neurological functions, and is high in operability and good in repeatability.

The invention relates to the technical field of medical equipment, in particular to a device for improving the treatment effect of a needle type electrode, and the device comprises a positioning and guiding subsystem, a treatment regulation and control subsystem, a safety monitoring subsystem and a display early warning subsystem. Gridding position information of the electrode treatment model is obtained through a positioning and guiding subsystem, electrode treatment points are determined based on the position information, and the matching degree of different electrode treatment points is analyzed through the positioning and guiding subsystem to obtain a target treatment point; a potential prediction model is arranged in the treatment regulation and control subsystem, the target treatment point is analyzed based on the prediction model to obtain the potential distribution condition of the target treatment point, and the treatment regulation and control subsystem regulates and controls the electrode treatment scheme based on the potential distribution condition; the safety monitoring subsystem monitors different target treatment points to obtain monitoring results; and the display early warning subsystem carries out real-time display and safety early warning on the needle type electrode treatment process in combination with the monitoring result, the electrode treatment model and the target treatment point.

The utility model relates to the technical field of animal husbandry midwifery, in particular to a multifunctional midwifery auxiliary device and obstetric table integrated structure which comprises an obstetric table and an obstetric table, and a groove is formed in one side of the obstetric table; the adjusting mechanism is arranged at the bottom of the inner wall of the obstetric table, and the adjusting mechanism extends to the other side of the obstetric table; the supporting mechanism is arranged on the adjusting mechanism, and the adjusting mechanism is used for driving the supporting mechanism to move left and right; by arranging the fixing plate, the one-way threaded rod, the first rotating handle, the moving table, the limiting groove, the transverse rod, the second U-shaped ring and the fixing rod, the hip of a cow can be fixed, meanwhile, the weight bearing force generated when a midwifery holds the middle portion of the fixing rod by hands can be effectively reduced, time and labor are saved when the device is used, the overall labor burden is reduced, and the working efficiency is improved. The operation is convenient and simple, and integrated connection is realized, so that the efficiency of aiding cow delivery is improved.

The invention discloses a low-intensity transcranial focused ultrasound cerebral apoplexy rehabilitation animal experiment method and system. The method sequentially comprises the following steps of ultrasonic parameter safety calibration, animal screening and baseline establishment, cerebral apoplexy animal model preparation and establishment, ultrasonic intervention treatment, neurobehavioral dynamic evaluation, tissue sample acquisition and processing and multi-modal tissue inspection. The system comprises a stimulation unit, a positioning unit and a control unit, and is used for realizing accurate and parameter-controllable ultrasonic intervention. By constructing a complete research closed loop from parameter safety verification and standardized model intervention to multi-dimensional effect evaluation, the problems of blind parameter selection, single evaluation system and nonstandard experimental process in the prior art are solved, and a set of standardized and repeatable research normal form is provided. The rehabilitation effect of the LIFU on the cerebral apoplexy can be comprehensively and objectively evaluated, and parameter optimization can be guided.

The utility model discloses an eyeball holder. The eyeball holder comprises a clamping assembly, the clamping assembly comprises a first clamping arm and a second clamping arm which are arranged in a crossed mode. A first clamping part is arranged at one end of the first clamping arm; a second clamping part is arranged at one end of the second clamping arm; the first clamping part is detachably connected with the second clamping part; the first clamping part and the second clamping part are each of a hemispherical structure with a containing space inside. The side wall of the first clamping part and the side wall of the second clamping part are each provided with at least two through holes. The device has the beneficial effects that when the eyeball is taken, the contact area between the first clamping part and the in-vitro eyeball and the contact area between the second clamping part and the in-vitro eyeball are large, and damage to the in-vitro eyeball is avoided; and along with the continuous tightening of the clamping assembly, liquid between the in-vitro eyeball and the clamping instrument is gradually separated through the through hole, so that the solution for soaking the in-vitro eyeball is prevented from hindering the work of the clamping instrument, and the in-vitro eyeball is prevented from sliding off from the clamping instrument.

The present application relates to the field of model construction, in particular to a kind of construction method of vertebral artery compression rat model, the inner diameter and blood flow velocity of normal rat vertebral artery are obtained by ultrasonic detection;Anesthetize rat, skin preparation, disinfection, incision, expose vertebral artery;According to the required compression degree, select the tissue clip of corresponding specification, fix on vertebral artery, keep vertebral artery in compression state;Suture incision, disinfection, bandage;After rat awakens, daily care, feed, until incision healing;Ultrasonic detection is carried out, the inner diameter and blood flow velocity of the vertebral artery of rat model are obtained, compared with the inner diameter and blood flow velocity of the vertebral artery of the same age healthy rat or rat before modeling, whether it meets the standard of successful modeling is judged.

The sampling detection device comprises a clamp body, a handle, a movable tool apron, a sample collection container and a cutting tool bit, the cutting tool bit comprises a tool and a butt joint, the butt joint is fixed to the end of the tool, the end of the butt joint is provided with an annular medicine storage bin, and an opening of the annular medicine storage bin faces the tool; a medicine storage and release structure is arranged in the annular medicine storage bin, an extrusion part is further arranged on the butt joint in a sliding mode, and an elastic pre-tightening part is arranged between the extrusion part and the butt joint. According to the sampling detection device, through a single handle driving source and an ingenious mechanical structure design, multiple functional units of cutting, sampling, transferring and dosing are highly integrated, multi-functional synchronous cooperation under coherent mechanical actions is achieved, the livestock ears serve as mechanical transmission media, and a dosing mechanism is automatically triggered in the movement process after sampling. Therefore, the time, the position and the triggering condition of liquid medicine release and the sampling action form internal and inevitable mechanical linkage and closed-loop feedback.