393results about "Surgical veterinary" patented technology

The utility model discloses a rat thrombus forming device which comprises a blood collecting assembly and a thrombus forming assembly, the blood collecting assembly is used for collecting blood of a rat, and the blood collected by the blood collecting assembly is conveyed to the thrombus forming assembly to prepare thrombus; the blood sampling assembly comprises a bottom plate and a top plate, the top plate is located over the bottom plate, a vertical supporting rod is connected between the center of the bottom plate and the center of the top plate, the top plate can rotate around the supporting rod, a plurality of round holes are formed in the top plate in the annular direction, and blood collecting pipes are vertically placed in the round holes; a liquid outlet is formed in the bottom of the blood collecting pipe, a blood transfer pipe is connected to the liquid outlet, and the head end of the blood transfer pipe extends to the thrombus forming assembly; the thrombus forming assembly comprises a rectangular forming groove, a forming template made of rubber is fixed to the bottom of the forming groove, pits with honeycomb holes are densely distributed in the forming template, the diameter of each pit is 2-3 mm, and the depth of each pit is 2-3 mm.

The invention belongs to the technical field of medical instruments, and discloses a surgical instrument for building a traumatic brain injury mouse model.The surgical instrument comprises a tube blade, a limiter, a holding piece and a vacuum device, the tube blade is used for rotationally cutting into brain tissue of a mouse, the limiter is movably installed on the tube blade in the axial direction of the tube blade, and the vacuum device is arranged on the holding piece; the limiting device is used for abutting against the skull of the mouse, the holding piece is provided with an airflow channel, one end of the holding piece is connected with the pipe blade, one end of the airflow channel is communicated with the pipe blade, and the vacuum device is connected to the other end of the holding piece and connected with the other end of the airflow channel. According to the surgical instrument for building the traumatic brain injury mouse model, the problems that an injury area is uncontrollable and a focus area is difficult to quantify and unify in the current traumatic brain injury mouse model building process can be solved, so that the success rate and efficiency of model building are improved to the maximum extent.

The invention discloses a myocardial ischemia reperfusion injury animal model construction method and application thereof in the technical field of model animal preparation. The method comprises the steps of hypertension and hyperlipidemia pretreatment, standardized ischemia-reperfusion operation, immune microenvironment regulation and control and model evaluation and verification. According to the invention, model construction is carried out on an integrated model operation table, so that a standardized ischemia-reperfusion process is realized, reproducibility of a myocardial injury degree and an immune cell infiltration rule of the model is realized, and suitability of the model and nano-drug research is enhanced; and a reliable tool is provided for research and development of targeted drugs such as immune cell membrane coated nanoparticles.

The invention discloses an isolator for establishing a bone nonunion animal model and a preparation method of the isolator, and relates to the technical field of medical instruments, and the preparation method comprises the following steps: step 1, obtaining a hydrogen-containing silicone oil prepolymer with a side chain containing Si-H bonds; 2, obtaining a silica gel macromonomer with an epoxy group on a side chain; step 3, obtaining an anti-inflammatory functionalized silica gel macromonomer; 4, reconstructing the skeleton model in a three-dimensional manner, and creating a defect isolator model; 5, adding the anti-inflammatory functionalized silica gel macromonomer obtained in the step 3 and a reactive diluent into a mixing tank, adding a cross-linking agent and polyether modified polydimethylsiloxane under mechanical stirring, stirring for 30 minutes, defoaming for 2 hours under 500Pa vacuum, and standing and curing in a darkroom after defoaming; and 6, a multi-material-box DLP photocuring 3D printer is adopted for isolator printing. According to the method, observation interference on the bone nonunion pathological process is fundamentally reduced, and high-fidelity modeling construction is achieved.

The invention discloses a low-intensity transcranial focused ultrasound cerebral apoplexy rehabilitation animal experiment method and system. The method sequentially comprises the following steps of ultrasonic parameter safety calibration, animal screening and baseline establishment, cerebral apoplexy animal model preparation and establishment, ultrasonic intervention treatment, neurobehavioral dynamic evaluation, tissue sample acquisition and processing and multi-modal tissue inspection. The system comprises a stimulation unit, a positioning unit and a control unit, and is used for realizing accurate and parameter-controllable ultrasonic intervention. By constructing a complete research closed loop from parameter safety verification and standardized model intervention to multi-dimensional effect evaluation, the problems of blind parameter selection, single evaluation system and nonstandard experimental process in the prior art are solved, and a set of standardized and repeatable research normal form is provided. The rehabilitation effect of the LIFU on the cerebral apoplexy can be comprehensively and objectively evaluated, and parameter optimization can be guided.

The present application relates to the field of model construction, in particular to a kind of construction method of vertebral artery compression rat model, the inner diameter and blood flow velocity of normal rat vertebral artery are obtained by ultrasonic detection;Anesthetize rat, skin preparation, disinfection, incision, expose vertebral artery;According to the required compression degree, select the tissue clip of corresponding specification, fix on vertebral artery, keep vertebral artery in compression state;Suture incision, disinfection, bandage;After rat awakens, daily care, feed, until incision healing;Ultrasonic detection is carried out, the inner diameter and blood flow velocity of the vertebral artery of rat model are obtained, compared with the inner diameter and blood flow velocity of the vertebral artery of the same age healthy rat or rat before modeling, whether it meets the standard of successful modeling is judged.

To provide a corner angle protector capable of preventing foreign matter from flowing into a corner angle during intraocular surgery, and a method for manufacturing the same.SOLUTION: The corner angle protector 1 includes a C-shaped member 2 that is placed at a corner angle through an incision site of an eyeball and is deformable in accordance with a shape of the corner angle in a state where the C-shaped member 2 is placed at the corner angle, and a thread 3 that is connected to one of opposing end portions of the C-shaped member 2 and can be pulled by a user to pull out the C-shaped member 2 placed at the corner angle from the incision site. The end of the C-shaped member 2 to which the thread 3 is connected is formed into a conical shape with a rounded apex.SELECTED DRAWING: Figure 1

The invention provides a TTA bone plate. The TTA bone plate comprises a bone plate body and a plurality of connecting parts connected to the bone plate body. The bone plate body is provided with a first end and a second end which are oppositely arranged in the height extending direction of the bone plate body, and the multiple connecting parts are located on the two opposite sides of the first end in the first direction respectively. The bone plate body is provided with a cavity and two windows formed in the two opposite sides of the bone plate body in the first direction, and the two windows communicate with the cavity. At least two supporting beams which are connected in a crossed mode are arranged in each window, at least one end of each supporting beam is connected with the inner wall of the corresponding window, and the supporting beams are obliquely arranged relative to the height extending direction of the bone plate body. The TTA bone plate not only can bear load pressure in the first direction, the second direction and the third direction, but also can bear load pressure in the direction forming an included angle with the first direction, the second direction or the third direction, so that the TTA bone plate is better in mechanical property, and the treatment effect of the TTA bone plate is better.

The present application relates to the technical field of medicine, and specifically includes the application of immunosuppressants in the preparation of drugs for treating pulmonary hypoperfusion type congenital heart disease, and further provides a premature infant pulmonary hypoperfusion animal model, and results show that lung development is significantly improved after using immunosuppressants. The present application verifies the Wnt pathway which is critical for lung development, indicates that immunosuppressants can improve the Wnt pathway changes caused by pulmonary hypoperfusion, confirms that immunosuppressants have a therapeutic effect on the pulmonary hypoperfusion type congenital heart disease caused by pulmonary hypoplasia, provides a new method and idea for improving lung development of such children, and thereby improves the long-term survival quality of congenital heart disease children in China.

The invention relates to the field of biological robots and neural engineering, and discloses an electrical stimulation control protection device for a turtle robot, which comprises a neck biocompatibility limiter, the neck biocompatibility limiter is of a sleeve structure and is composed of a rigid clamping part and a flexible limiting disc, the neck of the turtle robot penetrates through the rigid clamping part and the flexible limiting disc, and the flexible limiting disc is connected with the neck of the turtle robot. The flexible limiting disc abuts against the tortoise shell of the tortoise robot, the rigid clamping part abuts against the skull of the tortoise robot, the rigid clamping part limits head retraction of the tortoise robot, and the flexible limiting disc is used for ensuring the freedom degree of the tortoise robot in other directions. The mechanical protection, sealing performance and diversion performance of the waterproof sealing electrical stimulation sleeve module ensure the position stability of the electrode under long-term violent activity and water flow impact.

The application provides a construction system and device of a non-human animal model of tension pneumothorax, and the system comprises: a fixing unit for fixing the non-human animal; a first processing unit for puncturing a puncture needle to the parietal pleura, making a guide wire pass through the needle cavity of the puncture needle to enter the pleural cavity, and withdrawing the puncture needle; a second processing unit for inserting a catheter along the guide wire to make the catheter enter the pleural cavity, pulling out the guide wire, and fixing the catheter; a third processing unit for unidirectional air injection into the pleural cavity through the catheter; and a fourth processing unit for monitoring the range of pneumothorax, determining that the non-human animal model of tension pneumothorax is obtained when the lung sliding of the injection side chest is greater than or equal to 4 / 6 area and lung points are observed, stopping air injection, and obtaining the non-human animal model of tension pneumothorax. The construction success rate of the application is 100%, the construction process is rapid, repeatable and objective in determination, and the application can provide a rapid and repeatable platform for diagnostic research, decompression technology evaluation and training.

The invention belongs to the technical field of artificial insemination of bumblebees, and particularly relates to a male bumblebee sperm collecting device. The male bee seminal vesicle extraction platform is arranged on the base, the male bee seminal vesicle extraction platform is used for clamping the male bee seminal vesicle glass sheet, the male bee seminal vesicle glass sheet is used for placing a male bee seminal vesicle, and the male bee seminal vesicle extraction platform drives the male bee seminal vesicle glass sheet to move in the horizontal direction of the base; the insemination needle fixator is vertically arranged on the base in a lifting mode and used for fixing a glass needle, and the glass needle faces the male bee seminal vesicle glass sheet. Through the device, the extraction efficiency can be remarkably improved, so that the sperm exposure time is effectively shortened, and relatively high vitality is kept.

The invention discloses a method for constructing a lumbar spinal stenosis animal model, which comprises the following steps of: fixing an experimental rabbit at a prone position, and then forming a 2-3cm incision in the back side of the waist along the longitudinal direction so as to completely expose an intervertebral plate window between a first lumbar vertebra and a second lumbar vertebra; a disposable venous indwelling needle is used for puncturing the ligamentum flavum at the interlaminal window and then puncturing the disposable venous indwelling needle into the spinal canal, and then a metal needle core of the venous indwelling needle is pulled out, so that the soft catheter part of the venous indwelling needle is indwelled in the spinal canal; uniformly mixing PMMA bone cement powder and liquid to prepare fluid bone cement, extracting the bone cement by using an injector, slowly injecting the bone cement into the spinal canal, and removing the soft catheter after injection is completed; the injected bone cement is adhered to the inner wall of the spinal canal after being solidified, so that the inner diameter and the volume of the spinal canal are reduced in physical space, compression on spinal nerves is formed, and the animal model with lumbar spinal stenosis is obtained. The problem that in the preparation process of an animal model, nerves are prone to being damaged, and research test requirements cannot be met is solved.

The application provides a laboratory mouse tail skinner and an operating method thereof, which comprises a base, a cutting mechanism comprising a first assembly ring and a plurality of cutting assemblies, any cutting assembly comprising a first mounting seat and a cutting knife, the cutting knife being arranged on the first mounting seat and extending towards the center of the first assembly ring, and a skinning mechanism comprising a second assembly ring and a plurality of skinning assemblies, the second assembly ring being arranged concentrically with the first assembly ring, and the skinning assembly comprising a second mounting seat and a skinning knife, the skinning knife being connected to the second mounting seat and facing along the axial direction of the second assembly ring. The application replaces manual operation of a handheld blade with a mechanical structure, effectively avoiding the safety hazard of a sharp blade scratching the fingers of a laboratory worker. In addition, the application has a simple body structure and is convenient to assemble, can flexibly adjust the positions of the cutting assemblies and the skinning assemblies according to laboratory mouse tails of different specifications, has a wide range of applications, and has high practicality and promotional value.

The invention discloses an intelligent positioning type cow dystocia midwifery instrument and an operation method. The intelligent positioning type cow dystocia midwifery instrument comprises an intelligent positioning module, a midwifery execution module, a main control module and a man-machine interaction unit. The intelligent positioning module is used for acquiring the spatial position and posture data of a fetus in a birth canal of a cow, and comprises a stretching type ultrasonic positioning unit and a visual positioning unit; the midwifery execution module comprises an adjustable clamping mechanism and a traction driving mechanism and is used for implementing stable clamping and directional traction on a fetus; the instrument is more convenient to disassemble, clean and maintain due to the modular design, the 10.1-inch high-definition man-machine interaction touch screen integrates the functions of image display, parameter setting, early warning prompt and the like, the interface is visual, the training cost of operators is reduced, the sanitation and safety of operation are ensured through the double disinfection and sterility detection process, and secondary infection is prevented.

To provide a livestock birthing support device that can reduce the physical burden on caregivers assisting in the birthing of livestock. [Solution] The livestock birthing support device comprises a support body 10 having a lower body support section 11 for supporting the lower body of the caregiver and an upper body attachment section 12 for being attached to the upper body of the caregiver, and a powered traction means 20 attached to the support body 10 and wound with a pulling wire. The tip of the pulling wire extended forward from the powered traction means 20 is attached to the fetus inside the livestock mother's body, and the fetus can be removed from the mother's body by winding up the pulling wire.

The urethra minimally invasive knife comprises an insertion rod body, a cutting blade and an injection device, a containing groove is formed in one side of the insertion rod body in the length direction of the insertion rod body, one end of the insertion rod body is a closed end, the other end of the insertion rod body is an open end, the cutting blade is rotationally contained in the containing groove, and the injection device is arranged in the containing groove. The containing groove is communicated with an inner cavity of the insertion rod body, and the injection device is connected with the open end of the insertion rod body, so that liquid is injected into the insertion rod body through the injection device, and therefore the cutting blade is driven to rotate and be exposed out of the opening portion of the containing groove. The inserting rod body can carry the cutting blade to be inserted into the narrow pet urethra more smoothly, then liquid is injected into the inserting rod body through the injection device so that the cutting blade can be driven to rotate and be exposed out of the opening of the containing groove, then the narrow blocked part of the pet urethra can be cut through an operation, the structure is simple, operation is easy, and practicability is high. And the surgical cutting difficulty is effectively simplified.

The utility model discloses an animal limb operation strapping device, which belongs to the technical field of strapping hemostasis and comprises a limb sheath. A plurality of ribbons which are arrayed in the circumferential direction and are not connected with one another are connected to the upper portion of the limb sheath, a threading hole is formed in the end of each ribbon, and all the ribbons can be fastened together by penetrating binding belts into the threading holes; dense anti-licking puncture parts are fixed on the side surface of each ribbon from inside to outside in a penetrating manner, and the dense anti-licking puncture parts are like thorns on a hedgehog body. The binding device is used for binding and stopping bleeding at amputation or amputated limb during animal operation, a bandage is bound at a wound firstly, then the limb sheath is sleeved on the outer side of the bandage, and due to the fact that the anti-licking puncture part on the side face of the bandage is like a thorn on a hedgehog, animals can be effectively prevented from licking or scratching the bandage at the wound, so that the bandage is prevented from falling off, and the wound is prevented from being infected.

The utility model discloses a large-segment bone defect modeling tool which comprises an outer shell, a wire saw is arranged in the outer shell, two sets of rotating discs are arranged in the outer shell, and a driving structure used for rapidly driving the wire saw to move is arranged in the outer shell. The large bone defect modeling tool is provided with the trigger, when the tool is used, the trigger is pressed to push the connecting plate to move, the connecting plate drives the transmission shafts at the two ends to move and drives the fan-shaped plate to rotate through the transmission frame, the fan-shaped plate drives the transmission gear to rotate through the transmission gear block at the top of the fan-shaped plate, and finally the transmission gear drives the wiring cylinder to move through the rotary disc. And then a spring in a movable groove pushes a connecting plate to move in the reverse direction so as to pull the fret saw to move in the reverse direction, the fret saw can be pulled to move back and forth to achieve cutting by repeatedly pressing a trigger for many times, the fret saw can be conveniently and manually driven to conduct cutting, and the problem that driving cannot be conveniently conducted is solved.

The application discloses an adjustable skin wound suture device for livestock pathological sampling and relates to the technical field of medical suture devices.The slide hole plate is symmetrically installed at the axial center position of the end cover, and the inner wall of the slide hole plate is in threaded connection with the outer side of the screw rod.A stop rod is slidably installed on the inner wall of the slide hole plate, and the end of the stop rod, which is far away from the slide hole plate, is an inclined surface that is inclined from top to bottom to one side of the slide hole plate.The adjustable skin wound suture device for livestock pathological sampling is characterized in that the threaded connection of the screw rod and the slide hole plate can adjust the position of the stop rod according to the skin thickness of the suture position, adjust the bending depth of both sides when the skin nail is driven into the skin, and adjust the suture depth.When the device is lifted after the skin nail is driven into the suture position, the movement of the stop rod during resetting is matched with the inclined surface to press down the top of the skin nail, so that the skin nail cannot be separated after resetting, the stop rod still blocks the skin nail, and the suture position is pulled when the device is lifted.

There is described a methane oxidation device for oxidation of methane from a digestive cavity associated with an animal, the device comprising a methane oxidation unit for oxidising methane; wherein the methane oxidation unit comprises an inlet portion in fluid communication with the external environment, the inlet portion further configured to be mounted to a hollow member in fluid communication with a digestive cavity associated with an animal, and further when the methane oxidation unit comprises an outlet portion in fluid communication with the external environment, so that methane from a digestive cavity associated with an animal is oxidised in the oxidisation unit prior to release into the external environment. There is further described an installation gun for installing a methane oxidation device.

The invention discloses adjustable skin wound suturing equipment for livestock pathological sampling, and relates to the technical field of medical suturing equipment. The sliding hole plates are symmetrically installed along the center position of the axis of the end cover, the inner walls of the sliding hole plates are in threaded connection with the outer side of the screw rod, stop levers are slidably installed on the inner walls of the sliding hole plates, and the ends, away from the sliding hole plates, of the stop levers are inclined planes inclined towards one sides of the sliding hole plates from top to bottom; according to the adjustable skin wound suturing equipment for livestock pathological sampling, through threaded connection of the screw rod and the sliding hole plate, according to the skin thickness of the suturing position, the position of the stop rod and the bending depth of the two sides when a skin nail is nailed in can be adjusted, and the suturing depth can be adjusted; the top of the leather nail is pressed down by utilizing the cooperation of the movement of the stop lever during resetting and the inclined surface, so that the situation that the leather nail cannot be separated after nailing and resetting, the stop lever still blocks the leather nail, and the suturing position is pulled during lifting is avoided.

The utility model relates to the technical field of blood sampling devices for large animals, and discloses a sterile blood sampling device for large animals, which comprises a blood sampling needle, a blood sampling main pipe and a plurality of blood sampling branch pipes which are communicated in sequence, a peristaltic pump is arranged on the blood sampling main pipe, the blood sampling main pipe is communicated with a plurality of blood sampling branch pipes through a three-way pipe or a four-way pipe, the tail end of each blood sampling branch pipe is respectively connected with a blood sampling bottle, and a stop valve is arranged on each blood sampling branch pipe. According to the scheme, the peristaltic pump and the blood sampling branch pipes are combined, so that the blood sampling amount of a large animal in the end is ensured, meanwhile, the continuity of collecting blood for different project purposes is effectively improved, and the situation that the whole blood sampling amount is affected by blood coagulation and pipe blockage possibly caused by the fact that blood sampling bottles need to be switched and blood sampling is suspended in the midway is avoided.

The invention provides an electrode suitable for electroporation of early development chick embryos and a preparation method of the electrode. The electrode comprises an upper electrode and a lower electrode; the lower electrode comprises a root end, a middle end and a tail end which are connected with one another; the root end is a straight section which forms an angle of 35-45 degrees with the embryo; the middle end is arc-shaped, and the middle end is in a state of bending downwards and bending forwards at the same time relative to the root end; the tail end is a straight section horizontal to the embryo; the upper electrode is an electrode A suitable for small-range transfection or an electrode B suitable for large-range transfection; the electrode A comprises a straight section and a downward inclined section which are connected with each other and form an angle of 35-45 degrees with an embryo; and the electrode B comprises a straight section, a downward inclined section and a tail section which are connected with one another, wherein the straight section and the embryo form an angle of 35-45 degrees, and the tail section is horizontal to the embryo. By designing a reasonable electrode shape, accurate control of transfection area positioning is facilitated, and gene transfection in an ultra-small target range is realized.

The wound-free lamb castration device comprises an installation frame, an installation groove is formed in the installation frame, a threaded rod is rotatably installed in the installation groove, the outer surface of the threaded rod is in threaded connection with a movable plate, the outer surface of the movable plate is in sliding connection with the inner wall of the installation groove, and an extruder is installed on the outer surface of the movable plate; the motor B drives the threaded rod to rotate, then the movable plate slides back and forth in the mounting groove, the movable plate slides to drive the squeezer to squeeze the castrated portion of a lamb, seminal cord pinch-off is achieved, the castrating operation is completed, the squeezing speed and pressure on the seminal cord can be controlled more accurately, and the castrating effect is better. By means of the technical scheme, the risk of excessive force application or insufficient force application is reduced, the success rate and safety of an operation are improved, the wrist burden of an operator can be effectively relieved when multiple operations are conducted continuously, and operation errors caused by fatigue are reduced.

The invention discloses a zebra fish optic nerve ligation model construction system and device, the system comprises an acquisition module, an anesthesia module, a placement platform and a ligation module, the acquisition module is used for acquiring target basic information; the anesthesia module is used for determining target anesthesia parameters according to the target basic information; performing anesthesia according to the target anesthesia parameters; the placing platform is used for placing target zebra fish; the ligation module is used for clamping sclera by using a sterile tissue clamping tool with a fine tip so as to expose optic nerves; the eyeball is lifted through a sterile tissue clamping tool, and supporting pieces are arranged on the two sides of the eyeball to expand the operation space; separating the optic nerve from the blood vessel of the eyeball through a separation tool; ligating the optic nerve through a ligature; and removing the supporting piece, and resetting the eyeball to obtain the zebra fish optic nerve ligation model. By adopting the embodiment of the invention, the zebrafish optic nerve injury and regeneration model for simulating the chronic inflammation environment can be constructed.

A large animal modeling device for blunt wound research belongs to the technical field of medical experiment auxiliary tools and comprises a base, a first electric turntable and a second electric turntable. A large animal model; the lifting mechanism comprises a fixed rod, a movable rod and an air cylinder, the fixed rod is installed on the first electric rotating disc, a guide hole is formed in the fixed rod, the movable rod is in sliding contact with the inner wall of the guide hole, and the air cylinder is used for controlling the movable rod to move up and down; each blunt mechanism comprises a first transverse plate, a sliding block and a blunt device, the first transverse plate is fixedly connected with the movable rod, the sliding block is connected with the first transverse plate through a flexible part, and the blunt device is fixedly connected with the sliding block; the swinging mechanism comprises a rack, a swinging frame, a first motor, a fixing box and a locking assembly, the swinging frame is rotationally connected with the rack, the first motor controls the swinging frame to rotate, the fixing box is installed on the swinging frame, a sliding groove is formed in the top of the fixing box, and the locking assembly is used for locking the sliding block in the sliding groove.

The utility model relates to the technical field of electric coagulation haemostatic forceps, and discloses a miniature electric coagulation haemostatic forceps for animal test surgery, which comprises two connecting rods, the left sides of the two connecting rods are fixedly connected with extension rods, the exteriors of the two extension rods are connected with sliding sleeves in a sliding manner, elastic assemblies are arranged in the extension rods, and the elastic assemblies are connected with the sliding sleeves in a sliding manner. A sliding groove is formed in the left side of the interior of the extension rod, a hemisphere is fixedly connected to the bottom of the elastic assembly, a plurality of clamping grooves are formed in the bottom side of the interior of the sliding sleeve, an outer clamp body is fixedly connected to the left side of one connecting rod, and an inner clamp body is fixedly connected to the left side of the other connecting rod. According to the haemostatic forceps, after the hemisphere moves to the designated clamping groove position, the spring releases elastic potential energy, the hemisphere is pushed to reset and be clamped in the clamping groove in the bottom of the sliding sleeve, and therefore the length of the haemostatic forceps is locked. The length of the hemostatic forceps can be flexibly adjusted according to the requirements of different operation scenes, and a doctor can operate and use the hemostatic forceps conveniently.

To tag cattle with ear tags to indicate that the cattle have been inoculated against brucellosis.SOLUTION: An EID button tag for tagging and identifying cattle inoculated against brucellosis is provided which includes: a shell having a central opening; a transponder located in the shell; a male punch having a disc-shaped punch body and a stem extending distally from the punch body; and a vaccination identification button having a disc-shaped visual identification body and a mounting stem extending distally from the visual identification body, where the mounting stem is configured for irreversible insertion into the mounting channel. The punch body and the visual identification body are primarily tinted with different colors to provide visual identification of a head of cattle inoculated against brucellosis.SELECTED DRAWING: Figure 1

The invention discloses a method for constructing a chronic heart failure co-disease depression animal model through composite intervention, and belongs to the technical field of disease animal model construction. Firstly, a left anterior descending coronary branch ligation operation is performed on a mouse to construct a heart failure model, and then a depression model is constructed by constraining mouse superposition. After composite modeling, the systolic function of the mouse is obviously reduced, the activity ability is weakened, and myocardial fibrosis and hippocampal tissue damage occur, which indicates that the heart failure co-disease depression model is successfully modeled. The animal model construction method has a good application prospect in research on the pathogenesis, biomarkers and treatment means of heart failure co-disease depression.