1455 results about "Gastrointestinal tract" patented technology

The invention discloses lactobacillus kefiranofaciens IMAU12319 and application thereof, belongs to the technical field of microorganisms, and particularly relates to lactobacillus kefiranofaciens IMAU12319 which is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 8, 2024, the preservation address is No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, and the number is CGMCC No.31224. The lactobacillus kefiranofaciens IMAU12319 has the advantages that the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319, and the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319; the invention also relates to an application of the lactobacillus kefiranofaciens IMAU12319 in preparation of a probiotic agent capable of resisting gastrointestinal digestion. The strain disclosed by the invention not only shows good gastrointestinal tract endurance capacity, but also shows relatively strong potential in the aspects of intestinal adhesion, oxidation resistance and bacteriostasis, and has a wide application prospect in leavening agent strains.

The invention relates to the technical field of hippocampus extract, in particular to a hippocampus-sourced neuroprotection and anti-oxidative stress polypeptide and a preparation method thereof.The amino acid sequence of the polypeptide is shown as SEQ ID No.1. The preparation method comprises the following steps that 1, 500 g of fresh hippocampus is weighed, surface silt is removed, freeze-drying is conducted at the temperature of-60 DEG C, then the hippocampus is smashed through a smashing machine and sieved through a 20-mesh sieve, and the hippocampus extract is obtained; dialyzing with distilled water for 24 hours, and freeze-drying to obtain hippocampus powder; (2) weighing 200g of hippocampus powder, adding 1000ml of 95% ethanol, extracting for three times, removing fat-soluble substances, and extracting and freeze-drying precipitates by using a 1 mol / L NaCl solution under the condition of 4 DEG C in combination with ultrasonic assistance to obtain crude hippocampus protein; (3) the hippocampus crude protein is taken, the hippocampus polypeptide is prepared through an ultrasonic-assisted bionic digestion method, and the hippocampus polypeptide has the advantages of being specific in molecular structure, clear in action mechanism, stable in gastrointestinal tracts, capable of protecting nerves, resistant to oxidative stress and the like.

The invention belongs to the technical field of medical image processing, and particularly discloses a CT image segmentation and classification system based on class activation graph guidance, and the specific technical scheme is that the system constructs three functional complementary segmentation and classification networks of a coarse segmentation network, a classification network and a fine segmentation network; according to the method, collaborative optimization and performance improvement of classification and segmentation tasks are realized, a closed-loop optimization process is formed by a multi-task collaborative mechanism, and a bidirectional optimization path of segmentation guide classification and classification feedback segmentation is constructed by utilizing internal correlation existing in the segmentation and classification tasks; a coarse segmentation result containing tumor position information is used for enhancing the tumor discrimination capability of a classification network, a class activation graph generated by the classification network is converted into a spatial position weight, the segmentation network is guided to pay attention to a focus area with discrimination, a finer segmentation mask is generated, and through bidirectional information interaction, the tumor discrimination capability of the classification network is improved. The two dimensions of precise positioning and pathological discrimination of gastrointestinal tumors are remarkably improved.

The invention relates to the technical field of microorganisms, and discloses a multifunctional compound probiotic composition which comprises the following raw materials in percentage by mass: 40-50% of probiotics; 30%-40% of a microcapsule embedding material; prebiotics: 10%-25%; 1%-5% of auxiliary materials; the invention also provides a preparation process of the multifunctional compound probiotic composition. The preparation process comprises the following steps: S1, culturing the probiotics and detecting the activity; s2, a microencapsulation process: preparing a multi-layer microcapsule through a spray drying method and an emulsion crosslinking method, and embedding the probiotics; and S3, mixing the microcapsules with prebiotics and auxiliary materials to obtain the multifunctional compound probiotic composition. By adopting a composite probiotic compatibility technology and through the synergistic effect of different strains, the stability and functionality of intestinal microecology are enhanced, and the effects of improving the survival rate of probiotics in gastrointestinal tracts, promoting planting of beneficial bacteria and enhancing the immunoregulation ability are achieved.

The invention discloses a sea cucumber protein heptapeptide with functions of reducing blood pressure and blood sugar and application of the sea cucumber protein heptapeptide, and belongs to the technical field of bioactive peptide biology. The amino acid sequence of the sea cucumber protein heptapeptide is LPPGPFP. The sea cucumber protein heptapeptide can significantly inhibit the activity of ACE through a competitive inhibition mode, tolerates the gastrointestinal tract digestion environment, also has DPP-IV inhibition activity, can relieve hypertension and type 2 diabetes mellitus at the same time, and has an application value in preparation of products with the efficacy of reducing blood pressure and blood sugar due to multiple functions of the sea cucumber protein heptapeptide. The sea cucumber protein heptapeptide provided by the invention can significantly inhibit the activity of ACE through a competitive inhibition mode, has DPP-IV inhibition activity, has the characteristics of simple structure, safety, strong activity and the like, and has a wide application prospect.

To provide a device and a method for treating at least one of hypertension, pulmonary arteries, diabetes, obesity, heart failure, end-stage renal disease, digestive disease, urological disease, cancers, tumors, pain, asthma or chronic obstructive pulmonary disease by delivering an effective amount of a formulation to a tissue.SOLUTION: In embodiments of the present invention, the formulation may include at least one of a gas, a vapor, a liquid, a solution, an emulsion or a suspension of one or more ingredients. In embodiments of the present invention, amounts of the formulation and / or energy are effective in injuring or damaging tissue, nerves and nerve endings in order to relieve disease symptoms.SELECTED DRAWING: Figure 3B

The invention provides a medical image segmentation method and system based on spatial detail enhanced vision, and relates to the technical field of image processing. The method comprises the following steps: performing initial feature mapping on an input and preprocessed medical image to obtain an embedded feature map; the embedded feature map is input into an encoder for feature extraction, and multi-scale features are obtained; the encoder comprises a plurality of encoding stages, and the number of channels is doubled and the spatial resolution is halved through down-sampling operation between the encoding stages; the multi-scale features are subjected to up-sampling and spatial enhancement reconstruction step by step through a decoder, and a high-precision segmentation result is generated; wherein the decoder comprises a plurality of decoding stages, the decoding stages correspond to the encoding stages, and feature fusion is carried out between the corresponding stages of the encoder and the decoder through jump connection. According to the method, the boundary description precision and the segmentation robustness of the low-contrast image can be improved without increasing the linear complexity, and the method is suitable for medical image segmentation scenes of skin lesions, gastrointestinal polyps and the like.

The invention belongs to the field of bioactive peptides. The invention provides an angiotensin converting enzyme inhibitory peptide as well as a screening method and application thereof. The amino acid sequence of the inhibitory peptide is GKGLW and / or GDGLKW. According to the screening method of the ACE inhibitory peptide, the soybean protein is taken as a raw material, and the ACE inhibitory peptide is prepared and screened by adopting a targeted alkaline protease and chymotrypsin double-enzymolysis technology, so that rapid screening of the ACE inhibitory peptide is realized, and an experimental basis is provided for the structure-function relationship of the ACE inhibitory peptide. The inhibitory peptide provided by the invention has strong antihypertensive activity, high safety and no toxic or side effect. Digestive tract stability determination shows that the inhibitory peptide has good gastrointestinal tract stability in gastrointestinal tract digestion, and can still keep good activity after entering blood.

The invention relates to the technical field of artificial intelligence, and discloses a method and a system for dynamically predicting the risk of harm of feed mycotoxin to livestock and poultry. The method comprises the following steps: constructing a metabolic digital twin model based on a multi-organ coupling dynamics mechanism, connecting a gastrointestinal tract, a liver and a kidney through blood flow parameters to form a closed loop topology network, establishing a toxin migration rate equation, and verifying bioavailability; collecting feed toxin concentration and livestock and poultry physiological data in real time; after distributing the initial toxin load, iteratively generating target organ dynamic concentration distribution by adopting a Runge-Kutta method; an organ exposure index is calculated based on an organ specific hazard threshold, key organs are positioned, and low / high risk levels and toxin accumulation paths are output. The method solves the problems that a traditional static model is low in efficiency and poor in organ difference adaptability when being used for dynamically predicting the risk of harm to livestock and poultry caused by feed mycotoxin.

The invention provides a composition capable of penetrating an intestinal barrier by oral administration. The composition contains a carrier or an auxiliary material of a targeted Microflod cell Dectin-1 receptor, and one or / and a plurality of medicinal components / components, wherein the carrier or the auxiliary material of the target Microflood cell Dectin-1 receptor is beta-glucan, and the carrier or the auxiliary material of the target Microflood cell Dectin-1 receptor is beta-glucan. The effective components / components are traditional Chinese medicine effective components / components, or / and probiotics, or / and chemical medicine components, or / and biological medicines. The invention relates to a composition capable of realizing oral administration and penetrating through an intestinal barrier, which is based on the discovery of the inventor that the composition reaches colon through gastrointestinal tracts, targets colon Microflood cells, is taken by the Microflood cells, penetrates through the intestinal barrier and enters immune tissue Colonic Patches of a colon submucosa. Based on the research, the composition disclosed by the invention can be used for treating various diseases, such as ulcerative colitis, Crohn's disease, colon cancer, rheumatoid arthritis or bacterial pneumonia after being orally taken and absorbed by rectum.

The invention belongs to the technical field of biological medicines, and particularly relates to a plant lactobacillus YYY01 strain and application thereof in anti-inflammation and production of gamma-aminobutyric acid. The preservation number of the plant lactobacillus YYY01 provided by the invention is GDMCC (China General Microbiological Culture Collection Center) NO: 65147. The plant lactobacillus YYY01 has relatively good biological safety, relatively good survival in a gastrointestinal tract environment and certain intestinal tract adhesion capacity; the plant lactobacillus YYY01 can produce gamma-aminobutyric acid at high yield, and has the effects of resisting inflammation, regulating intestinal flora and enhancing immune barrier. Good application prospects are shown in the aspects of improving the yield of gamma-aminobutyric acid and preparing products containing gamma-aminobutyric acid.

The invention relates to the technical field of medical information systems, in particular to an intelligent gastrointestinal tract postoperative complication early warning system and a method thereof.The intelligent gastrointestinal tract postoperative complication early warning system comprises a data acquisition module, a data preprocessing module, a deep learning model module in communication connection with the data acquisition module, and a knowledge distillation module in communication connection with the data preprocessing module; the expert knowledge fusion module is in communication connection with the deep learning model module and is used for fusing expert knowledge into a mixed deep learning model; generating a lightweight prediction model; the dynamic weight adjustment module is in communication connection with the deep learning model module and the knowledge distillation module; the multi-task learning module is in communication connection with the deep learning model module; the early warning output module is in communication connection with the knowledge distillation module and the multi-task learning module and is used for generating complication risk early warning based on an output result of the lightweight prediction model; an early warning result and interpretive analysis are displayed through a visual interface, so that the early warning accuracy is improved, and more comprehensive patient state evaluation is provided for doctors.

The invention discloses a metal ion-mediated composite polysaccharide-based steady-state probiotic loading body as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. Sodium alginate and metal ions are coordinated and self-assembled through interaction of non-covalent supramolecules to form a metal ion-alginate skeleton network, functional guar gum is further filled, nano-scale aggregates are pushed to be spontaneously deposited on the surfaces of thalli, and a compound polysaccharide coating with excellent adhesion performance and steady-state performance is generated, so that the stability of the composite polysaccharide coating is improved. The independent coating of the probiotics is realized. The presence of metal ions increases the affinity of the biological material to the coated probiotics, and guar gum endows the probiotic delivery system with good adhesion characteristics. According to the probiotic loading body, the operation steps of an existing cell surface modification technology are simplified, the density is improved while the usage amount of biological materials is reduced, cells can be protected from being damaged by the gastrointestinal tract stress environment so as to maintain the activity of probiotics, and the colonization capacity and the biological activity of the probiotics in the intestinal tract are improved.

The invention discloses a protease preparation for preventing pet hair ball disease as well as a preparation method and application thereof, and belongs to the technical field of pet nutrition and health. The protease preparation for preventing the pet hair ball disease is mainly prepared from a compound enzyme, vitamins, trace elements, dietary fibers, probiotics, meat powder and a wall material, and the mass ratio of the compound enzyme to the vitamins to the trace elements to the dietary fibers to the probiotics to the meat powder to the wall material is (1-100): (0.1-1): (0.1-10): (1-70): (0.1-10): (10-60): (10-60). The protease preparation provided by the invention can effectively degrade hair and indigestible food, prevents the hair and food residues from forming pellets to block gastrointestinal tracts, prevents hair bulb syndrome from occurring in mechanism, and is simple in preparation process and wide in application prospect.

The invention discloses a lactobacillus casei KC88, and the preservation number of the lactobacillus casei KC88 is CGMCC (China General Microbiological Culture Collection Center) No.32107. Meanwhile, the invention also discloses a screening process and application of the strain. According to the lactobacillus casei KC88 provided by the invention, the expression of a Cdl gene with fusobacterium nucleatum can be down-regulated, and the expression of a Mgl gene of porphyromonas gingivalis can be down-regulated. At the same time, the lactobacillus casei KC88 can inhibit the production of pathogenic bacteria biological membranes and reduce the production of volatile sulfides. And the inactivated surface protein of the casei casei KC88 can inhibit the formation of a pathogenic bacterium biological membrane. Therefore, the lactobacillus casei KC88 viable bacteria and the thermally inactivated bacteria have the effects of relieving breath and improving gastrointestinal functions, and can be applied to the fields of medicines, health care products and the like.

The invention relates to the technical field of gastrointestinal tracts, in particular to a gastrointestinal tract ultrasonic virtual simulation nursing teaching platform which comprises a function module and a hardware configuration module. The function module comprises an anatomy learning module, an ultrasonic simulation module, a case training module and an examination and evaluation module; the hardware configuration comprises a VR head-mounted display, an ultrasonic simulator and an operation panel. The system has the advantages of safe and risk-free training, standardized process learning, immersive scene experience and instant feedback evaluation, a high-fidelity gastrointestinal tract ultrasonic virtual simulation teaching platform for critically ill patients is constructed, a 3D virtual model is constructed by using a virtual simulation technology, knowledge is presented dynamically and visually, real situations such as ICU wards and the like are simulated through VR, and real-time feedback evaluation of the gastrointestinal tracts of the critically ill patients is realized. The learning initiative and the sense of participation are stimulated; an ICU environment is virtualized, an unlimited'trial-and-error 'practice space is provided, the equipment cost is reduced, potential safety hazards are avoided, and space-time and safety barriers are broken.

The present invention pertains to the field of medical devices and introduces an enteral tube system designed for efficient and continuous delivery of fluids into a patient's gastrointestinal tract. The system integrates a one-way air valve along the tubular body, allowing ambient air to equalize negative pressure within the tube. This innovative feature enables the plunger of a syringe connected to the syringe port to be removed and the syringe refilled without disconnecting it, ensuring uninterrupted operation. The system also includes an enteral connector with user-controlled fluid flow pathways, enhancing versatility and precision during fluid administration. By addressing challenges such as negative pressure buildup and simplifying the refilling process, the invention reduces handling complexity and improves overall efficiency. The system is suitable for administering enteral fluids, medications, or diagnostic agents in healthcare settings, offering significant advantages over traditional enteral feeding systems.

The present invention is directed to orally administered compositions of topically acting corticosteroids for the treatment of inflammation of the gastrointestinal tracts such as eosinophilic esophagitis. The present invention also provides a method for treating conditions associated with inflammation of the gastrointestinal tract in an individual. The method comprises administering to an individual in need thereof a pharmaceutical composition of the present invention as orally disintegrating tablets comprising a topically active corticosteroid adsorbed onto a pharmaceutically acceptable carrier such as silicified microcrystalline cellulose.

The invention discloses protein-based nanoparticles as well as a preparation method and application thereof. The protein-based nanoparticles have a core-shell structure, the core is zein particles loaded with active drugs, and the shell comprises a trypsin inhibitor. The preparation method of the protein-based nanoparticles comprises the following steps: 1) preparing a zein dispersion liquid loaded with an active drug and a shell material aqueous solution; and 2) slowly adding the zein dispersion liquid loaded with the active drug into the shell material aqueous solution in a stirring state, and then carrying out rotary evaporation and freeze drying. The protein-based nano-particle has an excellent gastrointestinal digestion resisting effect, various hydrophobic active drugs can be effectively delivered to a lower digestive system, the stability and slow release efficiency of the loaded drugs are remarkably enhanced, and the protein-based nano-particle is good in biocompatibility, safe, non-toxic, simple in preparation process and suitable for industrial production. The method is suitable for large-scale industrial production and application.

The invention discloses tartary buckwheat insoluble dietary fiber additive type biscuits capable of reducing methylglyoxal and a preparation method of the tartary buckwheat insoluble dietary fiber additive type biscuits. According to the method disclosed by the invention, the tartary buckwheat bran is used as a raw material for continuous enzymolysis, so that the tartary buckwheat insoluble dietary fiber bound phenol with relatively high purity is obtained. Various phenolic substances are detected by the obtained tartary buckwheat insoluble dietary fiber combined phenol, so that the tartary buckwheat insoluble dietary fiber combined phenol has remarkable methyl glyoxal removal capacity, and most of methyl glyoxal removal capacity of residues digested by the stomach and the intestine can still be reserved, so that the tartary buckwheat insoluble dietary fiber combined phenol can continuously play a removal role in the gastrointestinal tract. The tartary buckwheat insoluble dietary fiber combined phenol is properly added into the biscuit, so that the content of methylglyoxal in the biscuit can be reduced, the intake of harmful substances in food by a human body is reduced, the occurrence of chronic diseases is slowed down, and the tartary buckwheat biscuit is beneficial to the health of the human body.

The invention belongs to the technical field of preparation of probiotic products, and relates to a lactic acid bacteria microcapsule as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing sodium alginate, modified montmorillonite and sterile water, cooling, adding a prepared lactic acid bacteria seed solution, and fully and uniformly mixing to form an emulsion; spraying the emulsion into a calcium chloride solution by adopting an extrusion method, stirring and curing to obtain microcapsules; and culturing the prepared microcapsule in a lactic acid bacteria liquid culture medium to obtain the lactic acid bacteria microcapsule after the culture is finished. The lactobacillus is effectively protected by adopting an extrusion-method single coating and post-coating culture process, so that the stability of the lactobacillus to a humid and hot environment and the gastrointestinal stress resistance are improved, and meanwhile, the release performance of the lactobacillus in the gastrointestinal tract is ensured. The problem that an existing microcapsule coating technology cannot achieve balance between improvement of the hygrothermal stability and gastrointestinal stress resistance of the probiotics and guarantee of good release performance of the probiotics in the gastrointestinal tract is solved.

The invention belongs to the field of nano preparations, and discloses a preparation method of nano microspheres coated with a nicotinamide mononucleotide shell membrane structure, which comprises the following steps: coating NMN in a pregel formed by chitosan and sodium tripolyphosphate, heating and stirring, adjusting the pH value to separate out chitosan-coated nano microspheres, drying to remove moisture on the surfaces of the nano microspheres, and drying to obtain the nano microspheres coated with the nicotinamide mononucleotide shell membrane structure. Then selecting a water phase and an oil phase in a proper proportion, preparing a chitosan / sodium alginate composite nano-microsphere with sodium alginate as an outer membrane by adopting an emulsion cross-linking method, and washing and drying to obtain the NMN nano-microsphere with a core-shell structure. According to the invention, NMN is coated in the nano-microsphere which is composed of two materials and has a nuclear membrane structure, so that the stability of NMN in a natural environment can be improved, hydrolysis of NMN by gastric acid and gastrointestinal tract enzyme in a drug delivery process can be prevented, the bioavailability of NMN is improved, and a drug sustained release effect is achieved.

The invention provides a spleen and stomach conditioning factor lactobacillus reuteri obtained by fermenting poria cocos powder. The strain has good acid resistance, cholate resistance and antibacterial ability. The invention further provides a probiotic preparation which has an obvious effect on improving the spleen and the stomach. In addition, the invention also provides a composite preparation composition containing Lactobacillus reuteri, and the composition effectively promotes normal peristalsis and digestion and absorption functions of gastrointestinal tracts by regulating the secretion level of gastrointestinal hormones, enhances the immunity of the organism, protects intestinal mucosa barriers, comprehensively improves the symptoms of weakness of the spleen and the stomach, and improves the overall health level.

The invention relates to the technical field of microorganisms, in particular to animal-derived pediococcus acidilactici and application thereof. The pediococcus acidilactici is classified and named as pediococcus acidilactici, the strain number of the pediococcus acidilactici is 12NN1, the pediococcus acidilactici is preserved in Guangdong Microbial Culture Collection Center on January 2, 2025, and the preservation number of the pediococcus acidilactici is GDMCC No: 65706. The strain is resistant to acid and cholate, has a remarkable inhibiting effect on growth and reproduction of pathogenic escherichia coli and staphylococcus aureus, is safe and non-toxic, does not have pathogenicity, and provides beneficial strain resources and biological materials for development of corresponding microbial preparations. Therefore, the compound can be used for preparing a biocontrol preparation for inhibiting the growth of pathogenic bacteria, preparing animal feed and preparing a preparation for preventing, improving and / or treating gastrointestinal tract pathogenic bacteria infection, so that the compound has a wide application prospect.

The present invention discloses a fermented mucus lactobacillus and its application. The present invention provides a fermented mucus lactobacillus A10 with high extracellular polysaccharide production, and its deposit number is CCTCC NO: M20242082. The fermented mucus lactobacillus A10 has strong bile salt and gastrointestinal digestion tolerance, and has obvious antibacterial effects on Escherichia coli and Staphylococcus aureus. The extracellular polysaccharide synthesized by the fermented mucus lactobacillus A10 is a Wzy type extracellular polysaccharide, which is a heteropolysaccharide composed of rhamnose, galactose, glucose, mannose, and glucuronic acid. It has good thermal stability and antioxidant activity, and can significantly scavenge free radicals. The fermented mucus lactobacillus A10 extracellular polysaccharide provided by the present invention can exert antioxidant activity by activating the Keap1-Nrf2 / ARE signaling pathway, and is expected to be developed into a natural antioxidant that interacts with Keap1-Nrf2 in a targeted manner.

The invention provides a gastrointestinal tract disease postoperative rehabilitation method and system based on big data, and relates to the technical field of gastrointestinal tract postoperative rehabilitation, historical rehabilitation patient data is acquired and clustered, historical rehabilitation patient clustering results and disease cluster similarities corresponding to different types of gastrointestinal tract disease clusters in the clustering results are acquired, and the disease cluster similarities are obtained; generating a gastrointestinal tract disease candidate set based on the disease cluster similarity, fusing a preset gastrointestinal tract disease standard vector with the gastrointestinal tract disease candidate set based on a gastrointestinal tract constraint fusion mechanism to generate a gastrointestinal tract disease rehabilitation prediction curve, and constructing a recombinant gastrointestinal tract disease candidate set; performing threshold calculation based on the recombinant gastrointestinal disease candidate set to obtain a dynamic threshold, obtaining a real-time data vector of a to-be-rehabilitated patient, matching a corresponding gastrointestinal disease rehabilitation prediction curve, comparing the real-time data vector of the to-be-rehabilitated patient with the gastrointestinal disease rehabilitation prediction curve, and implementing a corresponding strategy based on a comparison result. The method can assist a physician in generating a treatment scheme.

The invention provides clinical gastrointestinal decompression equipment for the digestive system department, and belongs to the technical field of adjuvant therapy. Comprising a mounting mechanism; a connecting arm is fixedly connected to the front face of the mounting mechanism, a power mechanism is mounted on the side, away from the mounting mechanism, of the connecting arm through bolts, and a rail frame is fixedly mounted on the side, away from the mounting mechanism, of the power mechanism. Through the arrangement, the equipment can be installed on the side edge of a sickbed during use, can adapt to different occasions, can adjust the height to meet the requirements of different patients, realizes the transverse and longitudinal synchronous displacement of the massage mechanism on the abdomen of the patient through the motor driving of the power and linkage mechanism, is large in massage range and excellent in massage effect, can replace manual massage, is not influenced by the external environment, and is suitable for popularization and application. The massage decompression mechanism can be used for hot compress massage, the temperature can be adjusted, the pressure of the gastrointestinal tract is further relieved, and better treatment experience is brought to a patient.

The invention provides a plant lactobacillus for promoting skeletal development in a growth period as well as application and a product of the plant lactobacillus, and belongs to the technical field of microorganisms. The invention provides a lactobacillus plantarum BL25 and a probiotic preparation thereof, the preservation number of the lactobacillus plantarum BL25 is CGMCC (China General Microbiological Culture Collection Center) No.33117, stachyose is adopted as prebiotics, and compared with other prebiotics, the lactobacillus plantarum BL25 can obviously promote the activity of the lactobacillus plantarum BL25. The plant lactobacillus BL25 and the probiotic preparation thereof have the effect of resisting the severe environment of gastrointestinal tracts, and can increase the length of femur and bone density, promote the development of a bone microstructure, improve bone metabolism, increase osteogenesis markers and reduce osteoclast markers, so that the skeletal development in the life growth period is promoted.

The invention provides cubilose peptide and a key preparation method of cubilose peptide maternity cubilose, and the preparation method of the cubilose peptide comprises the following steps: soaking dry cubilose in pure water at 0-4 DEG C according to a material-liquid ratio of 1: 10 for 2 hours, scattering, and spin-drying to obtain soaked cubilose; mixing the soaked cubilose with pure water according to a mass ratio of 1: (15-20), sealing, stewing at 121-123 DEG C for 12-15 minutes, cooling to room temperature, and repeatedly stewing once to obtain fresh stewed cubilose; carrying out an in-vitro digestion experiment on the freshly stewed cubilose, and simulating the digestion process of the freshly stewed cubilose in gastrointestinal tracts to obtain digested cubilose; and centrifuging the digested cubilose to obtain a supernatant, treating the supernatant, carrying out protein sequencing, and carrying out on-line database comparison to screen out peptide fragments with high matching degree and response value greater than 109, thereby obtaining the cubilose peptide. The cubilose peptide can significantly improve the proliferation rate of nerve cells, and provides a cell basis for the development of a nervous system.

The invention belongs to the technical field of feed additives, and particularly relates to slow-release fat powder for pigs and a preparation method of the slow-release fat powder. The slow-release fat powder for pigs comprises an intragastric adhesion-slow release layer, a small intestine forepart bile acid response controlled release layer and a small intestine posterior microbe response controlled release layer. The prepared slow-release fat powder for the pigs is good in stability, accurate release of different nutritional ingredients in different parts of gastrointestinal tracts of the pigs can be achieved, and powerful support is provided for sustainable development of the pig raising industry.