116 results about "Mating" patented technology

The present application relates to a kind of methods for mass propagation of tea corner miridae in laboratory, comprising the following technical steps: collecting tea garden tea corner miridae adult, cultivating fall kui seedling, putting seedling and leading insect, tea corner miridae habitat and feeding, mating and oviposition and nymph hatching and feeding.The present application uses fall kui seedling as the main body of feeding raw material, and the leaf of raw material is relatively thick, strong in harm resistance, not easy to dry, which helps to improve the hatching rate of tea corner miridae eggs, reduce the frequency of raw material supply and replacement, at the same time, the present application saves time and effort, can be realized in laboratory low cost and sustainability fast, efficient, mass propagation of tea corner miridae test insect source.

Disclosed are a genomic mating method for Huaxi cattle based on whole genome single nucleotide polymorphism (SNP) information and an application thereof. The method includes the following specific steps: step 1, extracting deoxyribonucleic acid (DNA) from to-be-hybridized Huaxi cattle individuals for genotyping; step 2, performing genotype data imputation to obtain high-density chip data; step 3, calculating an additive genetic relationship matrix, utilizing genomic best linear unbiased prediction (GBLUP) to obtain genomic estimated breeding values of five important economic traits of a to-be-hybridized Huaxi cattle population, and calculating a comprehensive selection index of the individuals; and step 4, using a genetic algorithm to construct a population optimal mating combination list. In the present invention, the breeding cost is greatly saved and an inbreeding level of offspring populations is reduced.

The invention discloses a sex attractant for bactrocera dorsalis and application of the sex attractant. The invention provides a sex attractant for bactrocera dorsalis. The sex attractant for bactrocera dorsalis comprises ethyl laurate, ethyl myristate, cis-9-hexadecenoic acid ethyl ester and ethyl palmitate. The sex attractant provided by the invention can effectively reduce the population density of male adult bactrocera dorsalis in the field, reduce successful mating of female adult bactrocera dorsalis, and reduce the reproduction rate of bactrocera dorsalis. The sex attractant developed by the invention is a novel bactrocera dorsalis attractant, can enrich compound resources for biological prevention and control of bactrocera pests and promote the development of a pest comprehensive prevention and control strategy towards the directions of high efficiency, economy, greenness and the like, and has very high application value.

A moving plate includes a plate body whose plate surfaces face a front-rear direction within a hood portion, front protruded portions protruding from the front surface of the plate body, and through holes extending through the plate body and the respective front protruded portions. The plate body is movable rearward, that is, in a direction approaching a male mating surface, from an initial position to a fitting position within the hood portion. A female housing includes opening peripheral surface portions expanding toward the front protruded portions, at opening edge portions of female cavities in a female mating surface, respectively. Each front protruded portion has a shape surrounding the entire periphery of the through hole on the front surface side of the plate body.

The invention discloses a lutjanus erythropterus breeding auxiliary method and system based on multi-character collaborative selection, and the method comprises the steps: carrying out phenotype data collection and gene detection, and constructing a standardized phenotype matrix and a genotype matrix; performing genetic parameter estimation and breeding value prediction based on the standardized phenotype matrix and the genotype matrix to obtain breeding value prediction information; the method comprises the following steps: analyzing an association relationship between characters of lutjanus rubripes to be bred, constructing a character association network, and carrying out multi-character dynamic weight configuration to obtain a dynamic weight set; performing genome selection index calculation and parent priority analysis according to the breeding value prediction information and the dynamic weight set to generate a parent selection priority list; and generating a candidate parent pool through the parent selection priority list, performing hybridization combination analysis, performing genetic gain prediction on the generated hybridization combination, and generating a hybridization auxiliary scheme. The genetic gain accuracy and balance of breeding of the lutjanus erythropterus are improved, the limitation of traditional experience selection and matching is broken through, and the breeding efficiency is improved.

The invention relates to a breeding method of Lingnan pigeons, belongs to the field of poultry genetic breeding, and mainly solves the problems of low growth speed and low meat yield of meat pigeons. The method comprises the following steps: firstly, selecting purebred male Shiqi pigeons and purebred female mimassa pigeons to establish a basic group, and naturally mating according to a male-female ratio of 1: 1 to obtain F1-generation hybrid pigeons; measuring the growth performance index and the reproductive performance index of the F1 generation, calculating the breeding index through an index selection method, and selecting and remaining the first 50% of individuals; mating the selected F1-generation male and female pigeons according to a ratio of 1: 1 to obtain an F2-generation, collecting gene samples of the F1-generation male and female pigeons, carrying out whole genome sequencing, and screening out core genes CTNNB1 and MYO9A for regulating and controlling the growth speed and the meat yield; f2-generation individuals conforming to target traits are selected and reserved based on genotypes for cross-crossing fixation to form two-line hybrid pigeons; and finally, carrying out three-way hybridization on the two-line male pigeons as male parents and American king pigeons as female parents to obtain commercial generation Lingnan pigeons. According to the method, the growth performance, the meat yield and the stress resistance of commercial generation pigeons are remarkably improved through combination of multi-generation hybrid advantage integration and a molecular breeding technology.

The invention relates to the technical field of gene engineering, in particular to a transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on a Tol2 transposition subsystem and a construction method of the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry. The preparation method comprises the following steps: S1, constructing a Tol2 transposon expression vector pTol2-ef1a-tjp1a-P2A-mCherry which contains an ef1a promoter, a tjp1a gene, a P2A peptide sequence and an mCherry reporter gene; s2, carrying out in-vitro transcription to prepare Tol2 transposase mRNA; s3, mixing the expression vector with Tol2 transposase mRNA, and microinjecting the mixture into the single-cell stage embryo of the zebra fish; s4, performing fluorescence screening on the F0 generation embryos surviving after injection to obtain the Founder fish with positive transgenosis; s5, after the F0-generation positive fish is bred to be sexually mature, an F1 generation is obtained through mating, transgenic positive individuals with stable inheritance are screened out after identification, and a transgenic zebrafish strain is established. According to the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on the Tol2 transposon system and the construction method of the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on the Tol2 transposon system, efficient integration of exogenous genes is achieved by utilizing the Tol2 transposon system, and the transgenic zebrafish line capable of being stably inherited to the F1 generation is successfully obtained.

The invention provides a teak camel moth artificial feeding feed and a teak camel moth artificial feeding method, the feed comprises the following components by weight: 8% of wheat germ powder, 4% of soybean meal, 4% of yeast powder, 2% of casein, 2% of sucrose, 1.8% of agar powder, 1.5% of cellulose, 0.8% of Webster salt, 0.6% of vitamin C, 0.2% of sorbic acid, 0.3% of methyl p-hydroxybenzoate, 0.1% of cholesterol, 0.1% of choline chloride, 0.05% of inositol, 0.01% of folic acid, and the balance of water. And the balance of deionized water. The invention further provides an artificial feeding method of teak camelmoths, and the artificial feeding method comprises the steps of 1, adult mating and egg laying, 2, egg disinfection and incubation and 3, larva rearing. The feed does not contain host material teak leaves, and full-artificial continuous large-scale feeding of the teak camelmoths can be achieved.

The present disclosure describes methods of using semiochemicals for insect pest control, and more specifically methods of using semiochemicals in agricultural fields for effectively disruptive mating in insect pests based on competitive attraction.

The application discloses an interactive pig breeding and mating method and a computer program product. The mating method is as follows: pedigree data, trait data and genotype data of multiple mating female pigs and multiple mating male pigs are acquired; comprehensive breeding values of each pig are calculated; the coefficient of kinship between each mating female pig and each mating male pig is calculated; all mating combinations are determined according to each mating female pig and each mating male pig, and mating labels corresponding to each mating combination are determined according to the comprehensive breeding value and the coefficient of kinship of each mating combination, wherein the mating labels include mating avoidance, mating suitability and mating optimization; an interactive list is generated according to each mating female pig and each mating male pig, and the mating label corresponding to the mating combination is displayed in a cell; and a mating plan is automatically generated according to the cell selected by a user in the interactive list. The application supports the user to interact with the list and automatically generates the mating plan based on the interactive process, which is very convenient and fast.

The invention discloses a preparation method and application of a tetraploid saccharomyces cerevisiae strain, and belongs to the technical field of bioengineering. Comprising the following steps: (1) knocking out an HO gene of diploid yeast LFN524, separating haploids through spore production, and identifying mating types of candidate haploid strains to obtain haploid strains with opposite mating types of haploids; (2) respectively culturing haploid strains of different mating types, inducing fusion of the haploid strains, and screening successfully fused diploid strains; and (3) inducing the successfully fused diploid strain to fuse again to obtain the tetraploid saccharomyces cerevisiae strain. The nucleic acid content of the strains 8-3 and 8-4 disclosed by the invention is obviously higher than that of a diploid stock plant, and the nucleic acid content can respectively reach 0.118 g / g-DCW and 0.096 g / g-DCW, which are respectively increased by 63.89% and 33.33%.

The invention provides a device for artificial cultivation of queen bees and a cultivation method thereof. The device comprises a plurality of cultivation units which are clamped with one another; a cultivation unit; the breeding box body, the small frame, the queen bee partition plate, the bottom grid plate, the clamping plate and the clamping groove are included, the breeding box body is specifically a square box body with an opening formed in the top face and side through openings formed in the two sides, the queen bee partition plate is clamped in the side through openings to complete the closing operation of the side through openings, and the bottom grid plate is clamped in the position, close to the bottom face, in the breeding box body. The two clamping grooves are formed in the top of the other long shaft side, opposite to the clamping plate, of the cultivation box body. In the queen bee breeding process, 8 days are saved, the utilization rate of the mating group is increased, the non-oviposition neutral period of the female queen bee in the four-area mating box is shortened, the supply amount of larvae is increased, the young bees of the mating group can continuously go out of a house, the fed bees of the mating group do not decline in the whole production season, the breeding quality of the produced queen bee is guaranteed, and the method is economical and practical.

The device comprises a transparent box body, a transparent box bottom and a cotton ball, an installation pipe is arranged at the top of the transparent box body, a plastic bag is placed in the transparent box body through the installation pipe, a bag opening of the plastic bag is arranged at a pipe opening of the installation pipe in a sleeved mode, and the cotton ball is arranged at the bottom of the installation pipe. The cotton ball is dipped with honey water and hung in the plastic bag, the cotton ball is connected to one end of the connecting rope, the other end of the connecting rope extends out of the mounting pipe and is fixed in the mounting pipe through the rubber plug, the connecting pipe penetrates through the rubber plug, the bottom end of the connecting pipe penetrates through the bottom of the rubber plug to be communicated with the transparent box body, and a through hole is formed in the rubber plug. The device has good sealing performance and can maintain high environmental humidity, mating and oviposition behaviors of adults can be conveniently observed through the transparent oviposition chamber, cotton balls dipped with honey water can be conveniently replaced to supplement nutrition for the adults, the oviposition duration of the female ostrinia furnacalis is generally prolonged, and the reproductive power of the female ostrinia furnacalis is improved.

The invention relates to a whole-course artificial cultivation method for bamboo borers. The method comprises the steps of female and male egg recognition and screening, artificial incubation, cultivation carrier preparation, larva transplantation, larva cultivation and the like. According to the method, the male and female eggs are accurately distinguished according to the appearances of the eggs, the high-quality female eggs are screened for incubation, and the technical defects of uneven matching of the male and female eggs, low mating rate, poor survival rate and limited yield in the existing natural cultivation are solved by means of controlling the incubation temperature and humidity, optimizing the cultivation carrier and transplanting conditions, artificially regulating the adult mating environment and the like; the survival rate of the bred bamboo borer eggs reaches 90% or above, the stocking survival rate of larvae also reaches 90% or above, the yield is 20 times that of an existing natural breeding technology, operation is easy, the cost is controllable, the method is suitable for large-scale popularization, the win-win situation of ecological protection and economic benefits is achieved, the method has extremely high practical value and market prospects, bamboo cannot be damaged by breeding, and disease and pest spreading is avoided. And economic benefits are achieved on the premise of protecting the natural environment, and the concept of green development is met.

The utility model relates to the technical field of fish breeding equipment, and discloses a device for mating and egg taking of zebra fish, which is characterized by comprising a box body, a horizontal partition plate and a vertical partition plate are fixedly connected in the box body, the horizontal partition plate and the vertical partition plate divide the box body into a mating chamber and an egg taking chamber, the horizontal partition plate is provided with a plurality of grilles, and the grilles are arranged in the mating chamber. And an egg taking device is arranged in the egg taking chamber. The mating chamber and the roe taking chamber are separated independently and communicated with each other, the roe taking device is arranged in the roe taking chamber to collect roes, in the roe taking process, the roes do not need to be taken away in advance, the water seepage prevention performance of the box body does not need to be considered, the roes can be taken out easily, the structure is simple, operation is convenient, and roe taking is efficient.

The invention relates to a secondary self-sexing mating method for meat quails and egg quails, and belongs to the technical field of self-sexing breeding of quails. The method comprises the following steps: (1) hybridizing an egg-type Beijing white-feather male quail and a meat-type Difark female quail to produce an F1 generation; male and female quails are identified according to the feather color of the F1 generation, the chestnut feather is a male quail, and the white feather is a female quail; or (2) hybridizing a Chinese yellow-feather male quail and a meat type Difankg female quail to produce an F1 generation; male and female quails are identified according to the F1 generation feather color, the chestnut feather is a male quail, and the yellow feather is a female quail. According to the method, Beijing white-feather quails and Chinese yellow-feather quails are respectively hybridized with meat type Difankg (FM line) quails, and the characteristics of white-feather and yellow-feather sex-accompanying inheritance are utilized to realize self-sex identification of the first generation of hybrid. And the first generation of hybrid has the characteristics of good meat production performance and high egg production performance of parents, can realize germplasm resource innovation of egg-type and meat-type quails, and has important practical application value for relieving supply and demand contradiction of high-quality quail meat in China and improving comprehensive benefits of quail breeding industry.

The invention provides a tussah tussah tall thousand-grain cocoon redirection breeding method which comprises the following steps: partitioning a breeding basic group of tussah, and stocking in a plurality of moth regions under the same ecological condition; after cocooning, measuring the weight of thousand cocoons by taking the moth area as a unit, and screening out a target moth area based on a measurement result; performing sex identification and whole cocoon quantity measurement on the silkworm cocoons in the target moth area, and screening out a target female individual set and a target male individual set according to data of the whole cocoon quantity; regulating and controlling the eclosion time of individuals in the target female individual set and the target male individual set to synchronize the eclosion time, and performing directional mating between the two sets according to a preset pairing rule based on the data of the total cocoon amount; and taking the filial generation obtained by directional mating as a breeding base population of the next generation, and carrying out continuous multi-generation directional breeding until a tussah silkworm strain with stable thousand-grain cocoon weight character is obtained. According to the method, a breeding path of primary screening, fine screening, directional mating and multi-generation closed loop is constructed, so that breakthrough increase of thousand-grain cocoon weight is realized.

To reduce the insertion / removal resistance between the terminal and the mating terminal. [Solution] The present invention discloses a terminal and a method for manufacturing a terminal. The terminal comprises a terminal body 10 having a mating portion 11 for mating with a mating terminal. A first bottom plating layer 11a is formed on the contact surface of the mating portion 11 of the terminal body 10, and a first silver-graphite plating layer 11c is formed on the first bottom plating layer 11a. Graphite particles are uniformly dispersed in the first silver-graphite plating layer 11c to improve the wear resistance and conductivity of the mating portion 11 of the terminal body 10. In the present invention, the silver-graphite plating layer has excellent wear resistance and conductivity, can significantly increase the number of mating cycles between the terminal and the mating terminal, and can reduce the insertion and removal resistance between the terminal and the mating terminal.

The invention relates to the technical field of artificial breeding of wild animals, in particular to an artificial hatching method for green peacocks. The method mainly comprises the steps of breeding cage building, parent selection and matching, hatching egg management, hatching environment control, embryonic development monitoring, hatching management and brooding management modules, and the hatching rate and the nestling survival rate are remarkably increased by simulating the native habitat environment, optimizing the hatching egg treatment process, precisely regulating and controlling hatching parameters and conducting brooding management in stages. According to the method, parents are screened by adopting a molecular marker technology, inbreeding mating is avoided, and the problem of embryo heat stress is relieved by innovatively combining egg cooling operation. The system is suitable for protecting endangered wild animals and provides a reference example for artificial breeding of rare pheasants.

The invention discloses a mating disruption arrangement, which includes clip-on means adapted to be connected with housing means, adapted to contain at least one pheromone adapted to disrupt mating of insects and / or pests, and said clip-on means is adapted to be clipped onto branches of trees and / or plants and / or stands and / or rods and / or wire and / or ropes; longitudinal locating means adapted to clip the clip-on means with the housing means high in branches of the trees and / or plants and / or stands and / or rods and / or wire and / or ropes. The housing means and the clip-on means can be integrally formed. The clip-on means and the housing means are located high in branches of the trees and / or plants and / or stands and / or rods and / or wire and / or ropes without the assistance of ladder(s).

The present application relates to a method for identifying a sex antagonistic hotspot region on a pig autosomal chromosome and application thereof. The method provided by the present application comprises the following specific steps: constructing a hybrid population F0, F1, F2 three generations, genome sequencing and SNP identification, transmission tracking of genetic material in the hybrid population pedigree, and identifying a sex antagonistic hotspot region. The method of the present application identifies that the Chr3:121M window is a sex ratio directional regulation molecular marker that is beneficial to the generation of male offspring in the Large White / Mini pig genotype individual. According to the method of the present application, the pig population sex can be predicted and regulated, and by screening and optimal mating of the genotype of the parent before mating, a plurality of generations is obtained by continuous cross without introducing exogenous blood relationship, so that the maximum value of the proportion of the genotype beneficial to the male or female is achieved under the condition that all genotypes of each generation are retained at the population level, and then the dynamic regulation of the sex ratio of the offspring pig population is achieved.

This invention discloses a sex pheromone antagonist for the poplar leafminer moth and its application. The active ingredient of the sex pheromone antagonist is cis-13-octadecene-15-ynyl alcohol. The sex pheromone antagonist of this invention can interfere with the orientation of male poplar leafminer moths towards females, causing males to become disoriented, thereby reducing the probability of encounters between males and females, interfering with mating, reducing the reproductive rate, and gradually controlling the extinction of the pest population, thus achieving the purpose of pest control. This invention can significantly reduce the damage caused by the poplar leafminer moth and is also environmentally friendly.

The present application relates to a kind of artificial feeding method of eucalyptus moth whole life cycle, belong to biological control technical field, egg mass is concentrated room temperature to hatch;Larva feeding environment is dark, temperature 23℃±2℃, humidity 70%±10%, 1~2 age larva group is fed with shiitake, 3~12 age larva is fed with fresh eucalyptus stem dry or branch dry;Pupa pre-feeding environment is same with larva, after body color changes into black brown, pupa is moved into emergence cage, keeps humidity 80%±10%, temperature 25℃±2℃, female pupa is 2℃~3℃ higher than male pupa, dark;Emergence adult is placed into net cage with length width height 9.6m×4m×8m and no light source in periphery, keeps female and male sex ratio in net ≤1:3 so that female and male moth copulation and lay eggs.The present application can make eucalyptus moth complete whole generation, its environmental condition is easy to control, feed is easy to obtain, simple operation, workload is small, especially solve the problem that adult mating is difficult and difficult to breed offspring.

The artificial efficient breeding method comprises the following steps that S1, host plants are planted in a spawning room, and the host plants are gramineae plants or asparagus plants which are easy to plant and have developed root systems; s2, collecting mature golden cicada nymphs which come out of the ground and are not subjected to eclosion, putting the collected mature golden cicada nymphs into an artificial climate box for artificial eclosion, and after eclosion and bee emergence, selecting parent adults; s3, putting the adults into a mating cage of an oviposition room according to a male-female ratio for mating, wherein an adult luring device consisting of a male cicada chirp generating device and a sex pheromone trap is arranged in the oviposition room. By arranging the adult luring device composed of the male cicada chirp generating device and the sex pheromone trap, the mating rate of the male cicada and the female cicada is synergistically increased, and technical support is provided for subsequently increasing the breeding rate of a cicada provenance; by means of the breeding method, efficient artificial breeding of the golden cicada seed source is achieved, and a seed source resource is provided for large-scale breeding of the golden cicada.

The invention discloses an artificial maturity promoting and fry breeding method for Peltella corii, which comprises the steps of cooling temporary rearing, maturity promoting, mating, spawning, hatching, high-density larva breeding, metamorphosis and the like, male snails and female snails are separately bred during maturity promoting, and a breeding scheme is optimized. According to the technical scheme, batch gonad maturation of parent snails according to plans is achieved, and the maturity is good; high-density breeding technologies such as fertilized egg hatching and larva breeding are overcome, a stable and efficient fry production process is established, a large number of high-quality, healthy and regular-specification young snails are provided for the breeding industry, and the provenance basis of the industry is fundamentally guaranteed.

The application discloses an indoor large-scale artificial breeding method of land firefly luciola cruciata, and relates to the technical field of insect breeding, in particular to an indoor large-scale artificial breeding method of land firefly luciola cruciata, which comprises the following steps: step one, construction of a breeding room; step two, dividing the breeding room into multiple breeding areas by using water ditches; step three, breeding of insects; step four, pupa period management; step five, management and mating and egg laying of adult stages; step six, egg hatching; step seven, breeding of newly hatched larvae and young larvae; and step eight, breeding of middle-aged and old larvae. The indoor large-scale artificial breeding method of land firefly luciola cruciata can keep a relatively constant and suitable temperature in summer and winter, keep a suitable breeding environment, greatly save manpower, achieve the goal of high-efficiency artificial indoor large-scale breeding of fireflies, greatly improve the breeding efficiency, and reduce the breeding cost of the fireflies.

The invention provides a method for increasing the hatching rate of eggs of military mating miridae and collecting newly hatched nymphs, and belongs to the field of biological control. The invention discloses a method for improving the hatching rate of eggs of military mating miridae and collecting newly hatched nymphs, in the hatching of the eggs of military mating miridae, main veins and side veins of platanus acerifolia leaves producing the eggs of military mating miridae are cut and placed in a culture dish, and the humidity in the culture dish is controlled, so that the hatching rate of the eggs of military mating miridae is remarkably improved and reaches up to 90%; transferring of newly hatched nymphs is completed by adding fresh platanus leaves into a culture dish; the collection of the newly hatched nymphs of the military mating miridae is completed by directly putting fresh platanus leaves with the newly hatched nymphs into an insect breeding box, so that the survival rate of the nymphs reaches 100%. The method is easy and convenient to operate, the situation that platanus acerifolia leaves are easily dried up or mildewed due to poor humidity control is avoided, and the hatching rate of the military mating miridae eggs is effectively increased; meanwhile, the situation that newly-hatched nymphs are transferred through a writing brush in a traditional method is avoided, and the survival rate of the newly-hatched nymphs in

The application discloses an ecological regulation model for indoor breeding of oncomelania, and relates to the technical field of snail breeding regulation. The model comprises a matching relationship between the number of indoor breeding units and the total amount of oncomelania breeding, which is used for realizing the standardized control of the initial oncomelania quantity of each breeding unit, ensuring the initial ecological balance of the experimental system, and determining the environmental regulation priority of the indoor oncomelania breeding unit based on the matching relationship. The application dynamically corrects the weight of the environmental parameter based on the risk intensity of different transmission stages of schistosoma, breaks through the bottleneck that the static prevention and control strategy is difficult to adapt to the change of the life cycle of parasites, and further realizes real-time monitoring of the fluctuation of biological characteristic parameters in the mating period, the pregnancy period and the reproductive period, so that the environmental regulation can accurately respond to the biological rhythm of the oncomelania population, ensures the sustainability of the indoor breeding of oncomelania to a certain extent, and reduces the risk of schistosomiasis transmission.

The application belongs to the technical field of biology, and particularly relates to a dsRNA of a target Helicoverpa armigera HaPburs gene and application thereof in prevention and treatment of the Helicoverpa armigera. HaPburs RNA interference mediated knockdown reduces the transcript level by 60.01%, and induces morphological abnormalities of pheromone glands (PG), which are irregular black protrusions; the silencing HaPburs reduces the main pheromone component Z 11-16: Ald content, while the male insect attraction rate and mating rate, cumulative egg production also significantly decrease, and the transcript level of the sex pheromone biosynthesis related genes HaPKA and HaACC is also significantly reduced. It is shown that HaPburs the genes participate in PG morphogenesis and regulate sex pheromone biosynthesis through a cAMP-PKA-ACC regulatory axis, thereby affecting the reproductive communication of the Helicoverpa armigera. The application provides a novel target and technical scheme for green and efficient prevention and control of the Helicoverpa armigera, and has important research and development value and application prospect.

The application provides a preparation method and application of an insect specimen, and belongs to the field of specimen preparation. The preparation method of the insect specimen comprises the following steps: collecting insects and observing, finding an insect mating pair, freezing the insect mating pair, fixing the frozen insect mating pair in a fixing solution, and transferring the insect mating pair into a storage solution to obtain the insect specimen. The preparation method of the insect specimen can fully expand the genital organs of the Phyllophaga, and maximally retains the mating state of the insects. After dissecting the prepared specimen, the lock-and-key theory in the reproductive isolation of insects can be verified. In addition, the Phyllophaga specimen prepared by using the method can be applied to the identification of female Phyllophaga.