The invention relates to a composition for preventing and controlling cucumber downy mildew and a preparation method thereof. The composition comprises, by weight, 3-5 parts of fluoride ether bacteria amide, 1-3 parts of Bituo microbicide, 1-3 parts of Coptis chinensis extract, 1-3 parts of garlic oil, 3-5 parts of medical stone, 0.1-0.5 part of pesticide fertilizer sustained release agent, 5-10 parts of wetting agent, 3-10 parts of dispersant, 25-35 parts of kieselguhr and 15-20 parts of water. The composition is reasonable in prescription and simple to prepare, is a novel environment-friendly preparation, and has significant effect in cucumber downy mildew prevention and control. During cucumber production, various pests exist at the same time frequently, and mixed application of four kinds of microbicides can enlarge the control range, enhance prevention and control effect, lower toxicity of the agents, prolong appropriate period for spraying, save prevention and control cost, reduce application times, enhance adaptability and delay development of target drug resistance.