80 results about "Parasitism" patented technology

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The application discloses a kind of carrier, kit and application for long-acting gene knockdown of animal parasitic nematode, belong to the field of animal parasitic disease prevention and control.The lentivirus vector in the application integrates specific coding sequence to the genome of the infected nematode, stably and continuously expresses short hairpin RNA (shRNA) in the nematode, efficiently produces primary single-stranded small RNA (siRNA), these siRNAs target homologous target gene mRNA to cause its degradation, realize stable and continuous gene silencing effect.The application breaks through the bottleneck of traditional animal parasitic nematode RNA interference (RNAi) technology with low efficiency and instability, significantly improves the timeliness of conventional RNAi, can be used to establish long-acting RNAi technology system of animal parasitic nematode, has application value in the research of host in vivo process such as nematode infection, parasitism and pathogenicity and animal parasitic nematode disease prevention and control.

The invention discloses a method for artificially breeding white moth gathering cotesia sinensis and applying the white moth gathering cotesia sinensis to biological control of fall webworm. According to the method, the key factors such as the optimal age, the optimal bee inoculation proportion and the optimal temperature of the hyphantria cunea larvae which are hosts for artificially breeding the cotesia cunea are determined, the parasitism rate and the offspring number of the cotesia cunea are effectively increased, the breeding period is shortened, and the bee breeding efficiency is improved. Meanwhile, an optimal scheme for prolonging the shelf life of the cotesia sinensis gather through low-temperature storage of the cocoons or newly-emerged adults is also defined. The new natural enemy product breeding application technology provided by the invention is specially applied to biological prevention and control of the fall webworms in the larval stage, not only enriches the types of existing fall webworm natural enemy products, but also provides a more comprehensive solution for biological prevention and control of the fall webworms, can reduce the excessive use of chemical pesticides, and is beneficial to environmental protection. And a powerful technical guarantee is provided for balance and health of an ecological system.

The invention belongs to the field of insect feeding and collection, and particularly relates to a device and method suitable for southern orius minutus feeding and predation function reaction determination.The device comprises an egg hatching chamber, a nymph feeding chamber and an adult feeding chamber which are connected through a first plastic channel and a second plastic channel respectively; the first plastic channel and the second plastic channel are each provided with a valve, a first filter screen is arranged in the first plastic channel, and a second filter screen is arranged in the second plastic channel. The device is simple to operate, convenient to observe, convenient to replace preys, convenient to collect and capable of reducing inter-species disability and measuring the predation and parasitism functions of insects.

The invention provides a method for improving the breeding efficiency of braconus splendens, and belongs to the technical field of insect breeding. According to the method, female bees which are 5-day-old, 6-day-old and 7-day-old and have no egg laying experience after eclosion are used for parasitizing third-year-old Asiatic corn borer larvae. And the parasitism rate, the eclosion offspring number and the female bee number of the braconus splendens are obviously improved. The method is carried out in a small space range, so that the encountering probability of the braconus splendens and the hosts is increased, the time of searching for the hosts by the braconus splendens is shortened, and the parasitic rate of parasitic wasps is increased. The method disclosed by the invention has important significance in efficiently breeding the cotesia macrorrhiza by using the Asiatic corn borer larvae indoors and guiding the field to release the cotesia macrorrhiza.

The invention discloses a method for breeding aphidius gifuensis, which adopts a specific breeding process, adopts specific male parent and female parent domestication with staged humidity and temperature and adopts different subculture schemes to construct a specific aphidius gifuensis breeding method, and aims to solve the problems that the existing aphidius gifuensis is easy to cause hyperparasitism when the number of cotton aphids is low, and the breeding efficiency is low. The cotton aphid is massively propagated, and the prevention and treatment effect is poor. According to the technical scheme, the breeding mode that the female parents and the male parents are alternately subjected to low-temperature domestication has a remarkable influence on the parasitism rate of F5-generation individuals obtained through breeding, compared with a control group, the parasitism rate difference of the aphidius gifuensis is remarkable, and the survival rate in low-temperature feeding and high-temperature feeding is also remarkably influenced; the stress resistance of the aphidius gifuensis can be improved, and the green and sustainable development of the cotton industry can be realized.

The invention relates to a method and a kit for detecting parasitism of parasitic wasps in vivo in a pupal stage, in particular to a method and a kit for detecting parasitism of drosophila pupae and horny hairy bees, and belongs to the technical field of molecular biology and insect parasitism detection. The detection method comprises the following steps: extracting genome DNA of a to-be-detected drosophila pupa tissue; carrying out PCR (Polymerase Chain Reaction) amplification by using a primer pair by taking the obtained DNA as a template; and detecting whether a basic group as shown in SEQ ID NO: 3 exists in a PCR product or not. A primer pair used in the detection method is composed of a forward primer TRdroS and a reverse primer TRdroR. The detection kit comprises: a primer pair; a reaction system required by the PCR reaction; the basic group as shown in SEQ ID NO: 3 is used as a positive control. The method has the following advantages: the detection specificity is high; the detection sensitivity is high; operation is simple and detection period is short; the method is suitable for large-scale sample detection.

The utility model relates to the technical field of plant cultivation, and discloses a parasitic plant aerial fog cultivation, parasitism and collection integrated device which comprises a carrying box, the carrying box is black, three opening and closing doors capable of being independently opened are arranged on the front face of the carrying box from top to bottom, and an aerial fog cultivation layer, a parasitism layer and an atomization layer are sequentially arranged in the carrying box from top to bottom; an illumination assembly is embedded in the top of the aerosol culture layer, and a plant fixing assembly is arranged at the bottom; a parasitic component is embedded in the bottom of the parasitic layer; a nutrient solution storage pool is arranged in the atomization layer, a fogging device is arranged in the nutrient solution storage pool and connected with a timing aerial fog regulator, the nutrient solution storage pool is communicated with a pipeline, and a switch valve is arranged at the tail end of the pipeline.

The present invention provides a system and a control method for water body rotation, drainage energy storage and power generation. During the water intake when generating power by water head and the process of emptying the water body in the caisson during energy storage, the water body in the caisson is rotated through a diversion pipeline to generate kinetic energy for energy storage. Due to the viscous force of water, the angular velocity and kinetic energy of the water body are reduced. Therefore, the bottom, wall of the frustum-shaped caisson and the water body are separated by gas to overcome the viscous force of water and increase the kinetic energy of the water body. At the same time, the cavitation jet bubble technology is adopted to overcome the biological parasitism of the water body, improve the stability of the caisson and the energy storage output power density of the caisson, and solve the problem of improving the drainage energy storage power density of each single underwater caisson. The rotational kinetic energy of the water body and the power generation of the water turbine quickly support the power grid and improve the energy storage power density of the caisson.

The invention belongs to the technical field of agricultural biological control, and discloses a parasitic wasp cross-season release method and system based on an ecological island and application. The parasitic wasp cross-season release method based on the ecological island comprises the steps that S1, plants with the trapping effect on target pests are planted in 2-3 point positions per mu on the periphery of an annual crop field; s2, before field planting of the trapping plants, pest prevention and control treatment is carried out on the trapping plants; s3, after the trapping plants are planted, pest traps are arranged at the planting points in a centralized mode, and target parasitic wasps are put in the planting points in a centralized mode. By means of the cross-season release method and system for the parasitic wasps, the overwintering survival rate of the parasitic wasps can be increased to 58.7% from 9.2%, the parasitic rate of target pests in the next year is increased by 32.5%, the annual release frequency is reduced by 1-2 times, and the cost is reduced by 40%.

The utility model discloses a cocoon bee parasitism and feeding device which is characterized by comprising a bee-keeping chamber, an insect receiving chamber and an insect feeding chamber which are sequentially connected from top to bottom and are of a cylindrical structure, movable doors are arranged at the upper end and the lower end of the bee-keeping chamber, a first hole plate is arranged at the top of the insect receiving chamber, and a supporting column is arranged in the insect receiving chamber. A plurality of partition plates are evenly and fixedly connected in the circumferential direction of the supporting column to divide the insect receiving chamber into compartments with the same number as the partition plates, an outward opening door is arranged on the side wall of each compartment, a drawing plate is arranged at the bottom of each compartment, a second hole plate is arranged at the top of the insect breeding chamber, and a soil layer is laid in the insect breeding chamber. The beekeeping chamber, the insect receiving chamber and the insect raising chamber are connected into a whole, manual operation steps are reduced, the insect receiving chamber is divided into a plurality of compartments through the partition plates, parasitic preferences of the cocoon bees on hosts of different species, different foodstuffs of the same host, different treatments of the same foodstuffs of the same host and the like can be tested, and foodstuffs are added through an outward opening door; and the parasitic process of the cocoon bees is prevented from being influenced by insufficient foodstuff in the parasitic process.

This invention discloses a precise and efficient breeding method for *Cyprinus dorsiflorus* based on habitat reconstruction. The breeding pond is divided into a parent mussel rearing area and a glochidia larva metamorphosis development area by a barrier wall consisting of an upper, switchable isolation net and a lower, fixed partition. The glochidia larva metamorphosis development area is constructed with a 20-mesh movable net forming two layers. After laying the substrate, mature parent mussels and host fish are introduced. The temperature is controlled at 22.0–24.0℃, and fertilization is promoted with a water flow of 0.08–0.15 m / s, while glochidia larvae are cultivated with a water flow of 0.06–0.10 m / s. Once the glochidia larvae in the brood pouch mature, the isolation net is opened, and the water flow is adjusted to 0.02–0.05 m / s to promote parasitism. Every 6–8 days, the parasitized and molted parent mussels and host fish are circulated and exchanged. After the molted mussels molt, they are threaded through the net and placed into the lower substrate for in-situ cultivation. This method achieves in-situ integration of fertilization, parasitism, and cultivation, significantly improving the parasitism rate and the survival rate of juvenile mussels. It is suitable for the preservation, propagation, and large-scale seedling cultivation of *Cyprinus cuspidatum*.

The present invention discloses a pentadecane parasitic wasp attractant and its application. Compared with existing pest attractants on the market, the pentadecane attractant provided by the present invention attracts beneficial parasitic wasps, eliminating growers' concerns about attracting more pests to their fields. Furthermore, the more beneficial insects there are, the better the field pest control effect. In addition to the pale-footed braconid wasp, the parasitic wasp attractant of the present invention may not only attract parasitic wasps of the Noctuidae family, but also other types of parasitic wasps, thereby increasing the number of parasitic wasps in the field and improving the parasitic wasp parasitism rate in the field, achieving the effect of parasitic wasps controlling field pests.

The present invention discloses a large-scale production device for Meteorus pulchricornis and its production method. The device includes, from top to bottom: a lid provided with a first screen; an upper box with an open top that mates with the lid and a second screen at the bottom; and a lower box with an open top that mates with the bottom of the upper box. According to the phototactic and upward tropic behaviors and the behavior characteristics of ventral clutching parasitism of Meteorus pulchricornis, the present invention designs a method of relatively isolating the host and the parasitoid above and below respectively by using a screen with a certain mesh number, so that the ovipositor of Meteorus pulchricornis can pierce through the mesh holes to reach the host and complete the oviposition behavior. This is beneficial to quickly separating the host and the parasitoid, and quickly transferring the host from the parasitism box to the breeding box, greatly improving the working efficiency during the parasitism process.

The invention provides a malania oleifera multi-dimensional ecological niche regulation and control planting method, relates to the technical field of plant cultivation, and aims to ensure that a stable parasitic relationship is established between malania oleifera and hosts before nursery stock outplanting through associated seedling culture in the same cup, solve the key problems that flesh and root systems are easy to rot and difficult to parasitize, and greatly improve the transplanting survival rate of nursery stocks. According to the method, the root ecological niche and the light ecological niche of the malania oleifera are subjected to multi-dimensional, dynamic and accurate regulation and control. According to the method, associated seedling culture and under-forest progressive light control are organically combined, a technical chain of seedling culture-transplanting-growth promotion integration is formed, the contradiction that the environmental requirements in the seedling stage and the growing stage are disjointed is solved, and healthy and rapid growth of malania oleifera from seedlings to adult plants is achieved. The selected host plants are common and easy to obtain, the method is convenient to popularize and apply in vast rural areas of malania oleifera suitable growing areas, and the method has important significance for protecting the rare species and developing the rural characteristic industry.

This invention discloses a method for artificially breeding *Mesochrysoma pineatus* to increase the mating rate and obtain more female offspring. The method significantly improves the mating and fertilization rate of female *Mesochrysoma pineatus* by adjusting the airflow and light intensity of the mating environment, thereby increasing the proportion of female offspring. This invention also determines the optimal host body weight, the ratio of infected wasps to bees, and the suitable temperature for parasitism and development during breeding, which can significantly increase the parasitism rate, obtain more offspring, and shorten the development cycle of the offspring, allowing for the reproduction of more parasitic wasps in a shorter time. This invention, while ensuring successful mating of adult *Mesochrysoma pineatus*, improves the parasitism rate of female wasps during artificial breeding, resulting in more offspring, especially female offspring, and shortening the breeding cycle, making it applicable to the large-scale production of this parasitic wasp.

The invention relates to the field of biological prevention and control, in particular to a biological prevention and control method for spotted wing drosophila based on double-organism temperature control and a research method. The temperature for releasing the Pseudocera pupae is 18 DEG C. At 14 DEG C, no parasitic wasps emerge from the spotted wing drosophila melanogaster; the intrinsic growth rate and the periodontal growth rate of the pupa are obviously lower than those of the spotted wing drosophila at the same temperature; the optimal net parasitism rate, the weekly-limited parasitism rate and the stable parasitism rate of the Pachyrhizus pupae are observed at the temperature of 26 DEG C, but simulation data show that the number of the Pachyrhizus pupae populations can be remarkably reduced under the condition that damage is reduced by releasing a large number of Pachyrhizus pupae at the temperature of 18 DEG C; the high reproductive capacity of the Drosophila melanogaster at 22 DEG C and 26 DEG C obviously reduces the biological control efficiency of the Drosophila melanogaster, which indicates that more than thousands of times of parasitic wasps are needed and the control time is too late; and the long egg laying days and egg laying period at 18 DEG C are beneficial to the biological control efficiency.

The present application provides a strain of Trichoderma asperellum TAZ19, a biocontrol agent and application thereof, wherein the Trichoderma asperellum TAZ19 is preserved in China Center for Type Culture Collection on October 25, 2024, and the preservation number is CCTCC M 20242341. The active ingredient of the biocontrol agent comprises the above-mentioned Trichoderma asperellum TAZ19 strain, and the Trichoderma asperellum TAZ19 strain is one or more of conidium, mycelium, fermentation filtrate or active extract thereof. The Trichoderma asperellum TAZ19 has excellent antagonistic effect on multiple plant disease pathogens such as Diaporthe phaseolorum, multiple Fusarium oxysporum, Valsa mali, and Botryosphaeria dothidea, has broad-spectrum antibacterial property, fast growth, high antibacterial activity, strong parasitism, and good salt and alkali tolerance, and has a growth-promoting effect on plants. The Trichoderma asperellum TAZ19 can be widely applied to the prevention and treatment of multiple plant diseases such as pine branch and stem rot, wheat stem base rot, tomato wilt, and has a wide application prospect.

The invention belongs to the technical field of breeding devices, and discloses a trichogramma dendrolimus breeding and receiving device. Comprising a collecting and releasing box and a plurality of parasitic cylinders, a plurality of through holes are formed in the upper end of the collecting and releasing box and used for inserting the parasitic cylinders into the collecting and releasing box, and the collecting and releasing box provides a replacement area for breeding bees; the parasitic cylinder comprises an inner cylinder and an outer cylinder, the inner cylinder is arranged in the outer cylinder, parasitic eggs are smeared on the outer wall of the inner cylinder after the inner cylinder is lifted out so that the parasitic egg smearing convenience can be improved, and the parasitic cylinder provides a parasitic breeding area for breeding bees; a base and a plurality of connecting rods are arranged at the bottom of the outer cylinder, the inner cylinder and the outer cylinder are fixedly connected through the connecting rods, meanwhile, a plurality of bee luring channels are formed through hollowed-out areas between the connecting rods, and therefore breeding bees in the collecting and releasing box enter a parasitic cylinder through the multiple channels to be subjected to parasitic breeding; the parasitism rate of trichogramma dendrolimus is increased, and the whole operation process is simple.

The invention relates to the technical field of biological agents, and discloses a biological agent for enhancing disease resistance, drought resistance and stress resistance of flax and a preparation method of the biological agent, and three-dimensional synergistic prevention and control of diseases are realized through three mechanisms of chemical bacteriostasis, nutrition competition and physical parasitism; according to the invention, the processing stability and the field colonization activity of the microbial inoculum are remarkably improved by combining the adaptability pretreatment of the strain and the carrier and the submodule dynamic adsorption process with the synergism of the functionalized carrier and the auxiliary agent, so that the viable count of the microbial inoculum is still kept at 2.1 * 10 < 8 > CFU / g or above after the microbial inoculum is stored for 12 months. Finally, in a complex field environment in which salt and alkali, drought and diseases are interwoven, the prevention and control rates of root rot and fusarium wilt of the flax respectively reach 90.5% and 89.1%, the water retention rate of soil is increased to 30.5%, and the survival rate of plants under high temperature stress is remarkably increased, so that the effect of providing comprehensive and stable ecological protection for the flax is achieved, and the application prospect is wide. And the popularization and application of the method in non-ideal agricultural production areas are effectively promoted.

The embodiment of the invention provides an ichthyophthirius multifiliis control method, system and device for marine fishes and a storage medium. The method comprises the steps that a first fish suction pump is controlled to suck to-be-controlled marine fishes in a first culture net cage into a treatment water cabin; to-be-controlled marine fishes are controlled through the control liquid medicine in the control water tank, and then controlled marine fishes are obtained; and a second fish suction pump is controlled to suck the prevented marine fishes into a second culture net cage from the treatment water cabin, and the first culture net cage and the second culture net cage are located on the traveling path of the prevention ship. To-be-prevented marine fishes in the first culture net cage are sucked into the treatment water chamber through the first fish suction pump for prevention and treatment, after prevention and treatment are completed, the marine fishes are sucked into the second culture net cage through the second fish suction pump, compared with a traditional prevention and treatment mode, ichthyophthirius multifiliis parasitism of the fishes can be effectively prevented and treated, economic losses are reduced, and the prevention and treatment efficiency is improved. The method can be widely applied to the technical field of breeding.

The application belongs to the technical field of genetic engineering, and particularly relates to application of HaSnRK2.5 gene in improving resistance of sunflower to orobanche parasitism. The complete HaSnRK2.5 gene sequence is obtained from the measured transcriptome data of sunflower, the HaSnRK2.5 gene is transferred into the sunflower, and the sunflower plant overexpressing the HaSnRK2.5 gene is obtained. Through the orobanche parasitism experiment on the obtained transgenic plant, it is found that the resistance of the plant overexpressing the HaSnRK2.5 gene to orobanche parasitism is significantly improved compared with the wild type sunflower. The finding has important value for molecular breeding and application of sunflower.

The invention provides a method for reducing the lethal risk of an insecticide to cotesia chilo borers, and belongs to the technical field of comprehensive treatment of agricultural pests, the method comprises the following steps: treating fourth-instar larvae of chilo suppressalis by adopting cyprofluramid; and parasitizing the fourth-instar larvae of the chilo suppressalis treated by the cyprofluramid by adopting the female cotesia chilo suppressalis in a ratio of 1: 1, and taking out the female cotesia chilo suppressalis after parasitism is completed. The method not only can reduce the adverse effect of the cyproflufen on the cotesia chilo borer, but also can effectively protect the natural enemy from being damaged by the pesticide, thereby maintaining the population dynamics of the natural enemy, being beneficial to maintaining the ecological balance of the farmland and reducing the pressure of the pesticide on the environment, being in line with the strategic direction of green agriculture and agricultural sustainable development, and having wide application prospects. The implementation method is simple and facilitates popularization and application.

The invention relates to application of n-tritetradecane in preparation of an aphidius gifuensis parasitic attractant, and belongs to the technical field of biopesticides. According to the technical scheme, the parasitism efficiency of the aphidius gifuensis to target pests can be improved, the parasitism potential of the aphidius gifuensis to unsuitable hosts can be expanded, therefore, the accuracy and stability of biological control are remarkably improved, meanwhile, a new technical means is provided for expanding propagation and utilization of the aphidius gifuensis, and the method is worthy of popularization and application. The method has an important application value for promoting the popularization of biological control in the agricultural field. Compared with a traditional mode depending on chemical pesticides, the method has the advantages that the problems of environmental pollution and pest resistance can be reduced, sustainable protection of an agricultural ecological system is achieved, development of a green prevention and control technology is promoted, and a new theoretical basis and an application tool are provided for comprehensive treatment of pests.

A method for mass rearing of Telenomus remus includes the following steps: (1) establishing a Telenomus remus population of a Corcyra cephalonica strain; (2) collecting Corcyra cephalonica eggs laid by Corcyra cephalonica on the day, and fixing the Corcyra cephalonica eggs on a sticker; (3) inoculating female Telenomus remus of the Corcyra cephalonica strain in the step (1) into the Corcyra cephalonica eggs in the step (2); and (4) after 24 h to 48 h of a parasitism, taking parasitic Corcyra cephalonica eggs out, and feeding the parasitic Corcyra cephalonica eggs under the following conditions: a temperature: 26±1° C., a relative humidity: 65±5%, and a photoperiod: L:D=14:10, where long axes of the Corcyra cephalonica eggs in the step (2) have a length of 0.48 mm to 0.60 mm. The method of the present disclosure has great significance for the long-term control of pests of the Noctuidae family.

The utility model relates to the technical field of beekeeping, in particular to a treatment device suitable for parasitic bee mites of bees, which comprises a protective shell and a separation component, the separation component is arranged in the protective shell and comprises an annular support frame I, the top of the annular support frame I is fixedly connected with a vibration motor, and the vibration motor is fixedly connected with the protective shell. The transmission end of the top of the vibration motor is fixedly connected with a connecting plate, the top of the first annular supporting frame is fixedly connected with a plurality of supporting rods, and the supporting rods are distributed in an annular array mode. According to the treatment device suitable for bee mite parasitism, when the device is used, a vibration motor can drive a mixing barrel to vibrate and shake through a connecting plate, powdered sugar on the top of a first fine dividing net can be evenly scattered on the surfaces of bees, and through the action of the powdered sugar, bee mites cannot be attached to the surfaces of the bees; the problems that a large number of bees die and bee products are polluted due to the fact that the proportion of chemical agents is not correct are solved, and the device can rapidly and conveniently remove bee mites.

The invention provides a method for obtaining polytype male and female interstitial Pingewei, and belongs to the technical field of arthropod reproductive mode and phenotype regulation, and the method comprises the following steps: feeding Pingewei carrying Ricktsia for several days by using a rifampicin solution, and feeding eggs to the Pingewei carrying Ricktsia for 24 + / -1h after feeding the rifampicin solution for parasitism, so as to obtain the polytype male and female interstitial Pingewei, and obtaining the polytype male and female interstitial Pingewei, so as to obtain the polytype male and female interstitial Pingewei, and the polytype male and female interstitial Pingewei. On the second day, the third day and the fourth day after the rifampicin solution is fed, polytypic male and female body surface types appear on the offspring, and the male and female body small bees are obtained. According to the method disclosed by the invention, the polytype male and female interbody Pingketsia is obtained by partially removing a symbiotic bacterium Rickketsia in the Pingketsia, so that a model for interaction between the Rickketsia and the host Pingketsia is favorably established, a foundation is laid for researching sex determination and differentiation of the host, and the research on the origin evolution of the sex type II of the insect and the formation reason of the male and female interbody is facilitated.

The invention discloses a method for reducing the infection risk of leeches in golden trevally culture, and belongs to the technical field of aquaculture. The method comprises the following steps: culturing golden trevally in a culture barrel, a cement pool or a net cage, and controlling the daily water exchange capacity to be more than 100%, the pH value of a water body to be 7.8-8.6, the dissolved oxygen to be more than 5.0 mg.L <-1 > and the ammonia nitrogen to be less than 0.2 mg.L <-1 >; the breeding density is reasonably controlled; carrying out polyculture on scatophagus argus in a certain proportion; reasonable bait casting; daily pool inspection and inspection; when the leech infection risk exists, the seawater salinity is adjusted regularly. According to the method, by comprehensively regulating and controlling the breeding environment and taking targeted measures, the infection risk of the golden trevally leeches can be remarkably reduced, leech parasitism is reduced, the breeding efficiency is improved, and the method has good application prospects.

The invention relates to the technical field of biological prevention and control of agricultural and forestry pests, in particular to an artificial breeding method of short-stalk long-tail encardia. The method comprises the following steps: by taking oak eggs as hosts for bee inoculation and a fructose aqueous solution as a sugar source, inoculating 3-day-old female bees which have reproductive memory and are copulated, and carrying out bee inoculation treatment twice; and breeding the tussah eggs on which the short-stalk long-tail bees parasitize, which are collected by the two-time bee inoculation treatment, so as to obtain the short-stalk long-tail bees. On the basis of reproductive memory and in combination with the biological characteristics of the short-peduncle long-tail bees, influence factors such as the most suitable mother bee day age, bee grafting proportion and time of the short-peduncle long-tail bees are determined, the phenomenon of low parasitic rate or over-parasitism caused by improper parasitic proportion or time is avoided, and the survival rate of the short-peduncle long-tail bees is improved. And therefore, the parasitism rate and eclosion rate of the short-stalk long-tailed encardia are improved.

The present invention relates to the technical field of biological control of agricultural and forestry pests, and particularly to an artificial breeding method for Torymus nagoyensis. The method comprises the following steps: using tussah eggs as hosts for wasp inoculation, using a fructose aqueous solution as a sugar source, and inoculating female wasps of Torymus nagoyensis at the age of 3 days with reproductive memory and having mated for two times of wasp inoculation treatment; cultivating the tussah eggs parasitized by Torymus nagoyensis collected from the two times of wasp inoculation treatment to obtain the Torymus nagoyensis. Based on reproductive memory and combined with the biological characteristics of Torymus nagoyensis, the present invention determines the influencing factors such as the optimal age of female wasps for wasp inoculation, the inoculation ratio and time of Torymus nagoyensis, avoiding the phenomena of low parasitism rate or over-parasitism caused by improper parasitism ratio or time, thereby improving the parasitism rate and emergence rate of Torymus nagoyensis.