27 results about "Parasitism" patented technology

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The invention relates to a method and a kit for detecting parasitism of parasitic wasps in vivo in a pupal stage, in particular to a method and a kit for detecting parasitism of drosophila pupae and horny hairy bees, and belongs to the technical field of molecular biology and insect parasitism detection. The detection method comprises the following steps: extracting genome DNA of a to-be-detected drosophila pupa tissue; carrying out PCR (Polymerase Chain Reaction) amplification by using a primer pair by taking the obtained DNA as a template; and detecting whether a basic group as shown in SEQ ID NO: 3 exists in a PCR product or not. A primer pair used in the detection method is composed of a forward primer TRdroS and a reverse primer TRdroR. The detection kit comprises: a primer pair; a reaction system required by the PCR reaction; the basic group as shown in SEQ ID NO: 3 is used as a positive control. The method has the following advantages: the detection specificity is high; the detection sensitivity is high; operation is simple and detection period is short; the method is suitable for large-scale sample detection.

The utility model relates to the technical field of plant cultivation, and discloses a parasitic plant aerial fog cultivation, parasitism and collection integrated device which comprises a carrying box, the carrying box is black, three opening and closing doors capable of being independently opened are arranged on the front face of the carrying box from top to bottom, and an aerial fog cultivation layer, a parasitism layer and an atomization layer are sequentially arranged in the carrying box from top to bottom; an illumination assembly is embedded in the top of the aerosol culture layer, and a plant fixing assembly is arranged at the bottom; a parasitic component is embedded in the bottom of the parasitic layer; a nutrient solution storage pool is arranged in the atomization layer, a fogging device is arranged in the nutrient solution storage pool and connected with a timing aerial fog regulator, the nutrient solution storage pool is communicated with a pipeline, and a switch valve is arranged at the tail end of the pipeline.

The utility model discloses a cocoon bee parasitism and feeding device which is characterized by comprising a bee-keeping chamber, an insect receiving chamber and an insect feeding chamber which are sequentially connected from top to bottom and are of a cylindrical structure, movable doors are arranged at the upper end and the lower end of the bee-keeping chamber, a first hole plate is arranged at the top of the insect receiving chamber, and a supporting column is arranged in the insect receiving chamber. A plurality of partition plates are evenly and fixedly connected in the circumferential direction of the supporting column to divide the insect receiving chamber into compartments with the same number as the partition plates, an outward opening door is arranged on the side wall of each compartment, a drawing plate is arranged at the bottom of each compartment, a second hole plate is arranged at the top of the insect breeding chamber, and a soil layer is laid in the insect breeding chamber. The beekeeping chamber, the insect receiving chamber and the insect raising chamber are connected into a whole, manual operation steps are reduced, the insect receiving chamber is divided into a plurality of compartments through the partition plates, parasitic preferences of the cocoon bees on hosts of different species, different foodstuffs of the same host, different treatments of the same foodstuffs of the same host and the like can be tested, and foodstuffs are added through an outward opening door; and the parasitic process of the cocoon bees is prevented from being influenced by insufficient foodstuff in the parasitic process.

This invention discloses a precise and efficient breeding method for *Cyprinus dorsiflorus* based on habitat reconstruction. The breeding pond is divided into a parent mussel rearing area and a glochidia larva metamorphosis development area by a barrier wall consisting of an upper, switchable isolation net and a lower, fixed partition. The glochidia larva metamorphosis development area is constructed with a 20-mesh movable net forming two layers. After laying the substrate, mature parent mussels and host fish are introduced. The temperature is controlled at 22.0–24.0℃, and fertilization is promoted with a water flow of 0.08–0.15 m / s, while glochidia larvae are cultivated with a water flow of 0.06–0.10 m / s. Once the glochidia larvae in the brood pouch mature, the isolation net is opened, and the water flow is adjusted to 0.02–0.05 m / s to promote parasitism. Every 6–8 days, the parasitized and molted parent mussels and host fish are circulated and exchanged. After the molted mussels molt, they are threaded through the net and placed into the lower substrate for in-situ cultivation. This method achieves in-situ integration of fertilization, parasitism, and cultivation, significantly improving the parasitism rate and the survival rate of juvenile mussels. It is suitable for the preservation, propagation, and large-scale seedling cultivation of *Cyprinus cuspidatum*.

The invention provides a malania oleifera multi-dimensional ecological niche regulation and control planting method, relates to the technical field of plant cultivation, and aims to ensure that a stable parasitic relationship is established between malania oleifera and hosts before nursery stock outplanting through associated seedling culture in the same cup, solve the key problems that flesh and root systems are easy to rot and difficult to parasitize, and greatly improve the transplanting survival rate of nursery stocks. According to the method, the root ecological niche and the light ecological niche of the malania oleifera are subjected to multi-dimensional, dynamic and accurate regulation and control. According to the method, associated seedling culture and under-forest progressive light control are organically combined, a technical chain of seedling culture-transplanting-growth promotion integration is formed, the contradiction that the environmental requirements in the seedling stage and the growing stage are disjointed is solved, and healthy and rapid growth of malania oleifera from seedlings to adult plants is achieved. The selected host plants are common and easy to obtain, the method is convenient to popularize and apply in vast rural areas of malania oleifera suitable growing areas, and the method has important significance for protecting the rare species and developing the rural characteristic industry.

This invention discloses a method for artificially breeding *Mesochrysoma pineatus* to increase the mating rate and obtain more female offspring. The method significantly improves the mating and fertilization rate of female *Mesochrysoma pineatus* by adjusting the airflow and light intensity of the mating environment, thereby increasing the proportion of female offspring. This invention also determines the optimal host body weight, the ratio of infected wasps to bees, and the suitable temperature for parasitism and development during breeding, which can significantly increase the parasitism rate, obtain more offspring, and shorten the development cycle of the offspring, allowing for the reproduction of more parasitic wasps in a shorter time. This invention, while ensuring successful mating of adult *Mesochrysoma pineatus*, improves the parasitism rate of female wasps during artificial breeding, resulting in more offspring, especially female offspring, and shortening the breeding cycle, making it applicable to the large-scale production of this parasitic wasp.

The invention relates to the field of biological prevention and control, in particular to a biological prevention and control method for spotted wing drosophila based on double-organism temperature control and a research method. The temperature for releasing the Pseudocera pupae is 18 DEG C. At 14 DEG C, no parasitic wasps emerge from the spotted wing drosophila melanogaster; the intrinsic growth rate and the periodontal growth rate of the pupa are obviously lower than those of the spotted wing drosophila at the same temperature; the optimal net parasitism rate, the weekly-limited parasitism rate and the stable parasitism rate of the Pachyrhizus pupae are observed at the temperature of 26 DEG C, but simulation data show that the number of the Pachyrhizus pupae populations can be remarkably reduced under the condition that damage is reduced by releasing a large number of Pachyrhizus pupae at the temperature of 18 DEG C; the high reproductive capacity of the Drosophila melanogaster at 22 DEG C and 26 DEG C obviously reduces the biological control efficiency of the Drosophila melanogaster, which indicates that more than thousands of times of parasitic wasps are needed and the control time is too late; and the long egg laying days and egg laying period at 18 DEG C are beneficial to the biological control efficiency.

The present application provides a strain of Trichoderma asperellum TAZ19, a biocontrol agent and application thereof, wherein the Trichoderma asperellum TAZ19 is preserved in China Center for Type Culture Collection on October 25, 2024, and the preservation number is CCTCC M 20242341. The active ingredient of the biocontrol agent comprises the above-mentioned Trichoderma asperellum TAZ19 strain, and the Trichoderma asperellum TAZ19 strain is one or more of conidium, mycelium, fermentation filtrate or active extract thereof. The Trichoderma asperellum TAZ19 has excellent antagonistic effect on multiple plant disease pathogens such as Diaporthe phaseolorum, multiple Fusarium oxysporum, Valsa mali, and Botryosphaeria dothidea, has broad-spectrum antibacterial property, fast growth, high antibacterial activity, strong parasitism, and good salt and alkali tolerance, and has a growth-promoting effect on plants. The Trichoderma asperellum TAZ19 can be widely applied to the prevention and treatment of multiple plant diseases such as pine branch and stem rot, wheat stem base rot, tomato wilt, and has a wide application prospect.

The invention relates to the technical field of biological agents, and discloses a biological agent for enhancing disease resistance, drought resistance and stress resistance of flax and a preparation method of the biological agent, and three-dimensional synergistic prevention and control of diseases are realized through three mechanisms of chemical bacteriostasis, nutrition competition and physical parasitism; according to the invention, the processing stability and the field colonization activity of the microbial inoculum are remarkably improved by combining the adaptability pretreatment of the strain and the carrier and the submodule dynamic adsorption process with the synergism of the functionalized carrier and the auxiliary agent, so that the viable count of the microbial inoculum is still kept at 2.1 * 10 < 8 > CFU / g or above after the microbial inoculum is stored for 12 months. Finally, in a complex field environment in which salt and alkali, drought and diseases are interwoven, the prevention and control rates of root rot and fusarium wilt of the flax respectively reach 90.5% and 89.1%, the water retention rate of soil is increased to 30.5%, and the survival rate of plants under high temperature stress is remarkably increased, so that the effect of providing comprehensive and stable ecological protection for the flax is achieved, and the application prospect is wide. And the popularization and application of the method in non-ideal agricultural production areas are effectively promoted.

The application belongs to the technical field of genetic engineering, and particularly relates to application of HaSnRK2.5 gene in improving resistance of sunflower to orobanche parasitism. The complete HaSnRK2.5 gene sequence is obtained from the measured transcriptome data of sunflower, the HaSnRK2.5 gene is transferred into the sunflower, and the sunflower plant overexpressing the HaSnRK2.5 gene is obtained. Through the orobanche parasitism experiment on the obtained transgenic plant, it is found that the resistance of the plant overexpressing the HaSnRK2.5 gene to orobanche parasitism is significantly improved compared with the wild type sunflower. The finding has important value for molecular breeding and application of sunflower.

The invention provides a method for reducing the lethal risk of an insecticide to cotesia chilo borers, and belongs to the technical field of comprehensive treatment of agricultural pests, the method comprises the following steps: treating fourth-instar larvae of chilo suppressalis by adopting cyprofluramid; and parasitizing the fourth-instar larvae of the chilo suppressalis treated by the cyprofluramid by adopting the female cotesia chilo suppressalis in a ratio of 1: 1, and taking out the female cotesia chilo suppressalis after parasitism is completed. The method not only can reduce the adverse effect of the cyproflufen on the cotesia chilo borer, but also can effectively protect the natural enemy from being damaged by the pesticide, thereby maintaining the population dynamics of the natural enemy, being beneficial to maintaining the ecological balance of the farmland and reducing the pressure of the pesticide on the environment, being in line with the strategic direction of green agriculture and agricultural sustainable development, and having wide application prospects. The implementation method is simple and facilitates popularization and application.

The invention relates to application of n-tritetradecane in preparation of an aphidius gifuensis parasitic attractant, and belongs to the technical field of biopesticides. According to the technical scheme, the parasitism efficiency of the aphidius gifuensis to target pests can be improved, the parasitism potential of the aphidius gifuensis to unsuitable hosts can be expanded, therefore, the accuracy and stability of biological control are remarkably improved, meanwhile, a new technical means is provided for expanding propagation and utilization of the aphidius gifuensis, and the method is worthy of popularization and application. The method has an important application value for promoting the popularization of biological control in the agricultural field. Compared with a traditional mode depending on chemical pesticides, the method has the advantages that the problems of environmental pollution and pest resistance can be reduced, sustainable protection of an agricultural ecological system is achieved, development of a green prevention and control technology is promoted, and a new theoretical basis and an application tool are provided for comprehensive treatment of pests.

The invention discloses a method for reducing the infection risk of leeches in golden trevally culture, and belongs to the technical field of aquaculture. The method comprises the following steps: culturing golden trevally in a culture barrel, a cement pool or a net cage, and controlling the daily water exchange capacity to be more than 100%, the pH value of a water body to be 7.8-8.6, the dissolved oxygen to be more than 5.0 mg.L <-1 > and the ammonia nitrogen to be less than 0.2 mg.L <-1 >; the breeding density is reasonably controlled; carrying out polyculture on scatophagus argus in a certain proportion; reasonable bait casting; daily pool inspection and inspection; when the leech infection risk exists, the seawater salinity is adjusted regularly. According to the method, by comprehensively regulating and controlling the breeding environment and taking targeted measures, the infection risk of the golden trevally leeches can be remarkably reduced, leech parasitism is reduced, the breeding efficiency is improved, and the method has good application prospects.

The invention discloses a boschniakia rossica symbiotic cultivation method with alnus japonica as hosts, and belongs to the technical field of boschniakia rossica cultivation The problem that boschniakia rossica symbiotic cultivation is low in survival rate and unstable in yield is solved. The method comprises the following steps: selecting a healthy alnus japonica middle-age forest with the tree age of 10-30 years as a host forest; oblique cuts are cut in the lateral roots of the alnus japonica at the positions 20-40 cm away from the trunk of the alnus japonica; mixing the boschniakia rossica seeds subjected to cold stratification treatment and hormone combined treatment with a biological adhesive to prepare seed paste; coating a root nodule symbiotic nitrogen-fixing actinomycete inoculant at a host root notch, filling the seed paste into the host root notch, covering the host root notch with wet humus soil, and compacting; the humidity of the covered wet humus soil is kept to be 15%-25%, low-concentration compound fertilizer and microbial agents are dressed in May, inoculation points are checked in June and September every year, and inoculation is supplemented if the parasitic rate is lower than 80%; after the boschniakia rossica germinates in the spring of the second year, collecting flower stems.

The invention relates to the technical field of biological prevention and control, and particularly discloses a method for field prevention and control of fall webworms by using brachycera pubescens, which comprises the following steps: collecting and domesticating brachycera pubescens species, raising hosts in a standardized manner, propagating brachycera pubescens, and regularly rejuvenating populations. And releasing the hyphantria cunea in a pupal stage at a density of 50-250 per mu under a suitable environmental condition. According to the method provided by the invention, by optimizing the bee pupae ratio and the field release density, the field parasitism rate of the brachycera majorana is remarkably increased, the highest parasitism rate can reach 62.5% and is far higher than the natural parasitism rate, and a new effective way is provided for green and sustainable prevention and control of fall webworms.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and particularly relates to a control method for inducing diapause of noctuid black egg bees. The method comprises the following steps: a, establishing a prodenia litura population and collecting new spawning blocks; b, continuously breeding the noctuid black-egg bees by taking the fresh prodenia litura eggs in the step a as hosts, and collecting female noctuid black-egg bees which are just subjected to eclosion; c, the prodenia litura eggs in the step a are inoculated with the noctuid black egg bee female bees in the step b for parasitism; and d, transferring to a diapause induction environment for treatment after parasitism. Under the conditions that the temperature is 8 DEG C and the photoperiod is 0L: 24D, or under the conditions that the temperature is 12 DEG C and the photoperiod is 8L: 16D, the diapause rate after 35-day induction treatment is relatively high, and the diapause rate reaches 97.5% or above. According to the method disclosed by the invention, the diapause rate of the black egg noctuid is remarkably improved through coordinated regulation and control of the temperature, the photoperiod and the induction time, and a key technical support is provided for long-term storage and large-scale application of the black egg noctuid.

The invention belongs to the field of plant disease and insect pest control, and provides a trichogramma dendrolimus natural enemy attractant and preparation and application thereof, and the trichogramma dendrolimus natural enemy attractant is a solution containing 2-hydroxy-propanamide and / or 2-propyl-furan. According to the attractant, the attraction effect on trichogramma dendrolimus is remarkably improved, the trichogramma dendrolimus is assisted to more quickly position target pest eggs, the parasitism rate of the trichogramma dendrolimus is increased, and finally the biological control effect of the trichogramma dendrolimus is improved.

This invention relates to formula I [Case 1] The present invention relates to the compound TIFF2026516766000021.tif30170 (wherein the formula, the variables have the meanings defined herein), compositions comprising the same, combinations of active compounds comprising the same, and their use for protecting growing plants and animals from attack or parasitism by invertebrate pests, as well as seeds comprising such compounds.

The invention discloses a method for reed bee propagation and pollination for island plants based on pittosporum tobira branches, which comprises the following steps of: constructing a reed bee propagation device: constructing a rainproof, sunshade and ventilated support structure, arranging the pittosporum tobira branches in the support structure as nest tubes, a plurality of stacked nest tubes in the supporting structure form a nest tube bundle for nesting of reed bees for expanding propagation; screening the conditions of reed bee development and natural enemy parasitism: checking whether the nest canals of the pittosporum tobira are nested and sealed by the reed bees, collecting the sealed nest canals, checking the conditions of reed bee development and natural enemy parasitism in the nest canals, and screening out the nest canals containing healthy reed bee bodies for subsequent introduction into the island and pollination for plants; transporting and releasing the reed bees: transporting the nest tube containing the healthy reed bee bodies to a target island and placing the nest tube on a new reed bee breeding device to release the reed bees. The method is low in cost, easy and convenient to operate, efficient, stable and specially designed for the island environment, and the breeding success rate of the reed bees and the pollination maturing rate of island plants are greatly increased.

This invention relates to the field of genetic engineering technology, specifically to sunflowers. HaMAPK6‑1 Application of genes in improving sunflower resistance to broomrape parasites. This invention obtains complete genome sequences from sunflower transcriptome data. HaMAPK6‑1 Gene sequence, and HaMAPK6‑1 The gene was transferred into the native plant, sunflower, using transient conversion technology, and overexpressed. HaMAPK6‑1 In sunflower plants containing the gene, an experiment was conducted on transgenic plants with orobanche parasitism, and it was found that overexpression of the gene was observed. HaMAPK6‑1 The genetically modified sunflower showed significantly enhanced resistance to broomrape parasites compared to wild-type sunflowers, a finding of great value for molecular breeding and application of sunflowers.

This invention discloses an apparatus and method for propagating parasitic wasps larvae. The apparatus includes a non-transparent bottom box, a transparent cover, and a non-transparent cover, arranged sequentially from bottom to top and capable of being sealed together. The non-transparent bottom box has an opening, and a first inlet on its side wall connects to its internal chamber. A second inlet corresponding to the internal chamber is provided on the transparent cover, such that when the transparent cover is placed over the opening of the non-transparent bottom box, the second inlet connects to the internal chamber. A third inlet corresponding to the second inlet is provided on the non-transparent cover. The apparatus also includes a first plug capable of sealing the first inlet and a first transparent adapter detachably connected to the first inlet. Furthermore, it includes a second plug capable of sealing the second inlet and a second transparent adapter that passes through the third inlet and is detachably connected to the second inlet. This apparatus and method offer significant advantages in terms of labor time, parasitism rate, and cocoon harvesting rate, significantly reducing propagation costs and improving propagation efficiency.

The invention belongs to the technical field of bee breeding, and particularly relates to a bee mite parasitism rate detection device which comprises a sealing cover detachably installed at the top end of a beehive, a plurality of evenly-arranged bee collecting and detecting mechanisms are fixedly connected to the top wall of the sealing cover, a controller is fixedly connected to the top end of the outer portion of the sealing cover, and the bee collecting and detecting mechanisms are electrically connected with the controller; the bee collecting and detecting mechanism comprises a bee collecting box, the bee collecting box is fixedly connected with the top wall of the sealing cover, the bottom end of the bee collecting box is detachably connected with a bee mite collecting part, a bee inlet mechanism is arranged on one side wall of the bee collecting box, and the outlet end of the bee inlet mechanism is located in the bee collecting box; a cleaning mechanism is arranged on the upper portion in the bee collecting box, and the bee feeding mechanism, the reagent adding mechanism and the ventilation mechanism are electrically connected with the controller. According to the method, the bee mite infection rate of the bee colony can be efficiently and accurately detected, and the bee mite infection condition of the whole bee colony can be accurately reflected.

The invention discloses a trichogramma gene editing method. The method comprises the following steps: (1) preparing a test bee species and hosts: preparing host tussah eggs; trichogramma is inoculated, and the trichogramma is inoculated for 6-8 hours; the parasitized tussah eggs are clamped in a fruit fly tube to be cultured; (2) preparation of pupae for injection: culturing in an incubator for 9-10 days, and splitting tussah eggs to obtain female pre-pupae of trichogramma; (3) preparing a solution for injection: mixing Cas9 and sgRNA, and incubating to form an RNP compound; (4) micro-injection: injecting the solution into hemolymph; (5) culturing female pupae in vitro after injection; (6) sub-packaging single heads of the eclosion female bees into finger-shaped tubes, and putting corcyra cephalonica egg cards for parasitizing until the offspring drones are all eclosion; and (7) screening mutants: observing mutation phenotypes under a stereoscope, and determining that gene editing succeeds through PCR (Polymerase Chain Reaction) detection. According to the method, the hosts are replaced by the tussah eggs from the rice moth eggs, so that mechanical damage caused in the dissecting process is reduced.

The utility model discloses a kind of parasitic wasp parasitic efficiency monitors, it is related to pest control technical field, its scheme includes: parasitic wasp rearing device and observation device: the parasitic wasp rearing device is used to rear parasitic wasp, first opening with openable and closable is provided on the side wall of the parasitic wasp rearing device, the first opening is used for parasitic wasp to pass through;The observation device is set to the periphery of the parasitic wasp rearing device, the observation device surface is provided with gauze for parasitic wasp to pass through, host larva is placed in the observation device, first monitor is provided at the top of the observation device.Such setting, the parasitic process of the device can be monitored to parasitic wasp, and its parasitic efficiency is counted, overall convenient to use, the data obtained is accurate, solve the time-consuming and labor-consuming, data error big problem of traditional manual observation and statistics, can provide effective data support for subsequent parasitic wasp warehouse release operation.

The invention discloses a breeding and feeding method for protagiosis longiscopicus, and belongs to the technical field of biological prevention and control of agricultural pests. The method comprises the steps of collection of parasitic wasp species, screening of host pupae, inoculation and culture of parasitic wasp offspring, preservation of adult wasps, cyclic utilization and reproduction of the host pupae, and recognition of parasitic host pupae. According to the method, the cinnamomum camphora pupae are determined as the optimal hosts through screening, key parameters such as the bee inoculation proportion, the temperature and humidity and the photoperiod are optimized, direct parasitism of pupae with cocoon shells is achieved, and the problem of population degeneration caused by manual cocoon stripping and host replacement is solved. According to the method, the life of adult bees can be remarkably prolonged, the parasitic efficiency and the breeding stability are improved, reliable technical support is provided for large-scale breeding and forest release application of the protagiospermus longiscopicus, and the method has important significance in promoting green prevention and control of camphor silkworms.

This invention proposes a screening method for biocontrol Trichoderma strains based on the dual criteria of oomycete parasitism and lethality. Belonging to the field of agricultural biotechnology, this invention addresses the technical problem that existing screening techniques often rely solely on the growth inhibition rate of confrontation cultures, making it difficult to distinguish between the antibacterial and lethal effects of biocontrol bacteria. This invention establishes a progressive screening system. The method uses highly pathogenic oomycetes, namely *Phytophthora capsici* (… Phytophthora capsici ) and ultimate pyrophyllium ( Globisporangium ultimum Using oomycetes as the target, and based on the determination of growth inhibition rate through confrontation culture, this invention creatively introduces a step of "pathogen re-isolation and detection of activity based on oomycete selective culture medium" combined with microscopic observation of hyperparasitism. This method effectively eliminates false-positive strains, significantly improves the accuracy of biocontrol strain screening and field efficacy, and is suitable for large-scale exploration of biocontrol resources.