62 results about "Trichoderma asperellum" patented technology

The invention discloses trichoderma asperellum TS7-1 capable of efficiently degrading macrolide antibiotics and application of the trichoderma asperellum TS7-1, and belongs to the technical field of microorganisms. The preservation number of the trichoderma asperellum TS7-1 is CGMCC (China General Microbiological Culture Collection Center) No. 41372. The Trichoderma asperellum TS7-1 (CGMCC No.41372) provided by the invention has high-efficiency degradation capability on various macrolide antibiotics (erythromycin, roxithromycin and azithromycin), the removal rates of the Trichoderma asperellum TS7-1, roxithromycin and azithromycin in 36h all reach 85% or above, and the Trichoderma asperellum TS7-1 has high-concentration antibiotic tolerance, degradation rate and degradation spectrum obviously superior to those of other strains in the prior art. The trichoderma asperellum TS7-1 is directly separated from a natural soil body which is not polluted by antibiotics, has no animal and plant pathogenicity hidden danger, has no microbial toxicity to degradation products of macrolide antibiotics, avoids secondary pollution, and has excellent environmental safety.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention relates to the technical field of agricultural microorganisms, in particular to trichoderma asperellum and application thereof.The trichoderma asperellum comprises at least one of the following trichoderma asperellum TA727, the trichoderma asperellum TA727 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO. 63859. The trichoderma asperellum has the advantages that the trichoderma asperellum can be used for preparing the trichoderma asperellum; the trichoderma asperellum is high in sporulation quantity and strong in acid resistance, and has a relatively good pathogenic bacterium inhibition effect under an acidic condition, and volatile substances of the trichoderma asperellum also have an antibacterial effect. The trichoderma asperellum can effectively inhibit pathogenic bacteria in an acid soil environment and soil-borne diseases caused by the pathogenic bacteria, meanwhile, industrial production can be facilitated, and the trichoderma asperellum has wide application prospects.

The invention discloses a composite microcapsule of bacillus subtilis and trichoderma asperellum, which is prepared by the following steps: embedding bacillus subtilis Pn1 and trichoderma asperellum Ta1 by adopting sodium alginate, and carrying out microencapsulation treatment, so that the controllable release of the bacillus subtilis Pn1 and the trichoderma asperellum Ta1 is realized; therefore, the stability of the composite biocontrol bacterium under adverse environmental factors is improved, meanwhile, the inhibition effect on root rot bacteria is enhanced, and the sodium alginate embedded bacillus subtilis Pn1 and trichoderma asperellum Ta1 have important application value in prevention and treatment of panax notoginseng biological root rot.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The invention provides bacillus cereus, the bacillus cereus is named as bacillus cereus, the bacillus cereus is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 66395. The invention further provides a synthetic flora, and the synthetic flora contains the bacillus cereus and the trichoderma asperellum MM7. The invention further provides a preparation method of the bacillus cereus and the trichoderma asperellum MM7. A synthetic flora constructed by the bacillus cereus and Trichoderma asperellum MM7 has a better effect of promoting the growth of banana plants, can be used for preparing a mixed bacterial agent or a microbial fertilizer, is beneficial to improving the economic benefits of the banana planting industry, and has a wide application space in green and sustainable development of the banana industry.

The invention discloses a trichoderma complex microbial inoculant capable of promoting wheat growth and preventing and treating wheat stem rot and application of the trichoderma complex microbial inoculant. The trichoderma complex microbial inoculant comprises a trichoderma asperellum strain XX-57 and a trichoderma harzianum strain PY-127, and the preservation number of the trichoderma asperellum strain XX-57 is CGMCC No. 41937; the preservation number of the trichoderma harzianum strain PY-127 is CGMCC (China General Microbiological Culture Collection Center) NO. 41938. The invention further specifically discloses application of the trichoderma complex microbial inoculant in promoting wheat seed germination and wheat growth and preventing and treating wheat basal stem rot, the trichoderma complex microbial inoculant is applied 10-20 days after wheat seedling emergence, and it is found that compared with single trichoderma inoculation, the trichoderma complex microbial inoculant has the promoting effect on wheat plant height, root length, SPAD value, fresh weight and dry weight; the control effect on wheat stem rot is better, and particularly the control effect is more obvious when pathogenic bacteria are inoculated in advance.

The application discloses application of Trichoderma asperellum strain YN4 and fungicides in prevention and treatment of plant fungal diseases. The application research shows that the Trichoderma asperellum strain YN4 has a good inhibitory effect on pepper anthracnose, rubber anthracnose, mango anthracnose, soybean anthracnose and the like. Further, compared with the single use of fungicides or the Trichoderma asperellum strain, the inhibitory effect of the combination of the two is better. Under certain conditions, the Trichoderma asperellum strain is combined with 10% difenconazole water dispersible granules, 70% thiophanate-methyl wettable powder or 450g / L prochloraz emulsion in water, and has a synergistic effect. Not only can the use amount of fungicides be greatly reduced, but also the inhibitory effect on plant pathogenic fungi can be further improved, the incidence can be reduced, the inhibition on pathogenic bacteria can be enhanced, the use and residue of chemical pesticides can be reduced, and technical support is provided for the selection of fungicides for pepper anthracnose and the like and the combination of bacteria and drugs.

The invention discloses an efficient detoxification and nutrient recovery method for aerobic composting of fungus-enhanced erythromycin fermentation fungus residues, and belongs to the field of solid waste disposal and resource reutilization. The method comprises the following steps: mixing erythromycin fermentation mushroom dregs subjected to high-temperature sterilization treatment with corn straw powder, inoculating trichoderma asperellum, and carrying out aerobic composting fermentation. According to the method, aerobic co-compost of erythromycin fermentation mushroom dregs and crop straws is strengthened by inoculating an antibiotic degrading microbial agent taking trichoderma asperellum as a core, and an organic fertilizer which is high in nutrient content and is non-toxic and harmless is produced, so that an efficient, economical and environment-friendly solution is provided for solving the problem of resource utilization of the erythromycin fermentation mushroom dregs.

The invention relates to the field of environmental pollution treatment and microorganisms, and discloses a preparation method of a trichoderma asperellum straw fermentation extracting solution and application of the trichoderma asperellum straw fermentation extracting solution in oxytetracycline pollution remediation or crop growth promotion. The method comprises the following steps: 1) performing solid-state fermentation on a trichoderma asperellum T1 seed solution to obtain a fermentation product; and 2) drying the fermentation product, adding the dried fermentation product into the leaching solution for leaching, and filtering to obtain the trichoderma asperellum straw fermentation extracting solution rich in trichoderma asperellum spores, enzyme and reducing sugar. The invention finds that the trichoderma asperellum T-1 has new application of repairing oxytetracycline pollution and promoting crop growth, can efficiently degrade oxytetracycline at normal temperature especially in the aspect of repairing oxytetracycline pollution, and has practical application value. Furthermore, compared with a non-extracted trichoderma asperellum leavening and pure trichoderma spores, the trichoderma asperellum straw fermentation extracting solution obtained by a specific process has a better oxytetracycline pollution repairing effect and a better crop growth promoting effect respectively in comparison with the non-extracted trichoderma asperellum leavening and the pure trichoderma spores.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

The present invention discloses Trichoderma asperellum ZC3 and its application in the degradation of Zanthoxylum bungeanum branches. This strain has a fast growth rate and strong degradation ability. In a liquid filter paper strip culture medium, after culturing at 28 °C for 7 days, the filter paper strip completely disintegrates; in a solid culture medium of Zanthoxylum bungeanum branch powder, after culturing at 28 °C for 40 days, its lignin degradation rate for Zanthoxylum bungeanum branches reaches 78.11%, and the degradation rates of cellulose and hemicellulose reach 82.58% and 75.84% respectively. Under a scanning electron microscope, obvious fiber breakage and structural disintegration phenomena can be seen in the Zanthoxylum bungeanum branches, showing a high degradation ability. In addition, the lignin-degrading bacterium ZC3 has good tolerance to the leaching solution of Zanthoxylum bungeanum branches.

The invention relates to a composition with sleep aiding and skin repairing effects and a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a composition with sleep aiding and skin repairing effects. The composition is prepared from the following components in parts by weight: 72 to 114 parts of compound plant essential oil, 3 to 5 parts of ceramide NP and 1 to 3 parts of sodium hyaluronate, the compound plant essential oil is prepared from lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil and rhodiola rosea essential oil; the rhodiola rosea essential oil is obtained by fermenting a mixed strain composed of kluyveromyces lactis and trichoderma asperellum and then extracting essential oil. The research shows that the mixed strain fermentation can synergistically improve the extraction rate of the rhodiola rosea essential oil. In addition, the compound plant essential oil provided by the invention can also synergistically improve the anti-inflammatory, moisturizing and anti-allergy repair capabilities of the composition and has the effect of improving sleep disorders, so that the composition has better sleep aiding and skin repairing effects at the same time.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

The invention relates to a trichoderma asperellum sourced Cerata-plaatanin (CP) family protein CP3 and application thereof, and belongs to the technical field of biological prevention and control of plant diseases. The CP3 protein is derived from Trichoderma asperellum, belongs to a Cerato-platanin family and has a conservative CP structural domain, the amino acid sequence registration number of the CP3 protein is XP024756165.1, and the registration number of a coding gene of the CP3 protein is 36612810 (Gene ID). The protein is a secreting type protein, has signal peptide and can be positioned in tomato cell nucleuses. The invention proves that the disease spot area of tomatoes infected with Botrytis cinerea can be reduced by 71% after the CP3 protein is exogenously applied; after tomatoes are treated by the trichoderma asperellum CP3 overexpression strain, the area of gray mold scabs is remarkably reduced, and the CP3 gene knockout strain has no disease-resistant effect. The CP3 protein and the trichoderma asperellum strain expressing the CP3 protein can be used for preparing biological agents for enhancing the botrytis cinerea resistance, application modes comprise rhizosphere application, root system inoculation and the like, adverse effects on tomato growth are avoided, and a new effective means is provided for green prevention and control of the botrytis cinerea.

The invention discloses a method for preventing and treating diseases and pests of corncobs by mixed use of insecticidal diatomite, metarhizium anisopliae and trichoderma. According to the invention, the insecticidal diatomite, the metarhizium anisopliae CQMa421 dispersible oil suspending agent and / or the trichoderma asperellum GDFS1009 spore suspension are combined and sprayed on the ostrinia nubilalis larva body or ears or leaves infected by the ostrinia nubilalis and ear rot pathogen, the control effect on the ostrinia nubilalis reaches 60-80%, and the control effect on the ear rot caused by fusarium verticillium reaches 60-100%. The effect of preventing and controlling ostrinia nubilalis larvae and ear rot obviously exceeds that of an equivalent applicable single preparation, and synergistic green prevention and control of ear ostrinia nubilalis and ear rot are realized; the method is a novel green prevention and control technology for corn ear diseases and insect pests.

The invention discloses a cultivation method for promoting the growth of rhizoma bletillae and the accumulation of active ingredients, which comprises the following steps: carrying out foliage spraying on planted annual rhizoma bletillae seedlings by adopting a mesoporous silica loaded jasmonic acid methyl ester solution as a growth promoting agent, and meanwhile, applying trichoderma asperellum microcapsules to the roots of the annual rhizoma bletillae seedlings, the purposes of promoting growth of rhizoma bletillae and accumulation of active ingredients are achieved; by adopting the method, the growth of the rhizoma bletillae can be remarkably promoted, the content of active ingredients such as total polysaccharides, total phenols and total flavonoids in the rhizoma bletillae is effectively increased, meanwhile, the content of antioxidant ingredients is greatly increased, the yield and quality are both improved, and the method has important application value in rhizoma bletillae quality improvement cultivation.

The invention discloses a biological control method for cotton fusarium wilt. The invention provides a combined bacterium or a complex microbial inoculant containing the combined bacterium. The combined bacterium is prepared from AT actinoplanes (Actinoplanes) Q9316 (CGMCC (China General Microbiological Culture Collection Center) No.35443) and Trichoderma asperellum (Trichoderma asperellum) F2609 (CGMCC No.42159). The invention further provides a preparation method of the composite microbial inoculant. The fermentation liquid independently fermented by the trichoderma asperellum F2609 and the AT actinoplanes Q9316 provided by the invention has an inhibition effect on pathogenic bacteria of cotton fusarium wilt, and the bacteriostatic activity strength of the fermentation liquid jointly fermented by the trichoderma asperellum F2609 and the AT actinoplanes Q9316 exceeds that of the fermentation liquid fermented by single bacteria. The invention has important significance for biological prevention and control of cotton fusarium wilt.

The invention provides trichoderma asperellum HN-097 with high antagonism to hylocereus undulatus and application of the trichoderma asperellum HN-097. Wherein the plate confrontation result of the trichoderma asperellum HN-097 on the dragon fruit canker is 73.90%. The inhibition effect of the trichoderma asperellum HN-097 on the volatile substances of the dragon fruit canker is 8.13%. The inhibition effect of the trichoderma asperellum HN-097 on the non-volatile substances of the pitaya is 8.20%. The strain is identified as trichoderma asperellum through morphology and molecular biology. The preparation of the trichoderma oil suspending agent comprises 84.55% of soybean oil serving as an optimal oil carrier, 0.1% of boric acid serving as an optimal spore germination accelerant, 0.3% of ascorbic acid serving as an optimal ultraviolet protective agent, 0.05% of xanthan gum serving as an optimal adhesive and 5% of lignin calcium sulphonate serving as an optimal dispersing agent. When the trichoderma asperellum HN-097 oil suspending agent is used for preventing and treating the pitaya ulcer disease, the control effect of the trichoderma asperellum HN-097 oil suspending agent on pitaya fruits is 82%, and the control effect of the trichoderma asperellum HN-097 oil suspending agent on stalks is 51.18%. The trichoderma asperellum HN-097 can obviously reduce the incidence rate and the disease index of the dragon fruit canker, is green and environment-friendly, effectively reduces the use of chemical agents, and promotes the sustainable development of the dragon fruit industry.

A Trichoderma asperellum HM-8 of the invention has the dual ability to antagonize Erwinia and Fusarium solani f. sp. batatas. The microbial agent prepared from the Trichoderma asperellum HM-8 strain can efficiently control sweet potato root rot and ginger stem basal rot. The spore count of Trichoderma asperellum HM-8 is not less than 108 CFU / g.

The invention discloses application of a compound microbial agent in prevention and treatment of leguminous crop root rot and crop growth promotion, and relates to the technical field of microorganisms, and the key point of the technical scheme is as follows: the compound microbial agent is a compound of a strain of Penicillium spinulosum LS001 and a strain of Trichoderma asperellum LS002. The strains are analyzed and identified to be P.spinulosum LS001 and T.asperillum LS002 based on a culture form and an ITS sequence, and plate antagonism proves that the two strains and sterile filtrate thereof have a very good inhibition effect on fusarium oxysporum; pot experiments prove that a single strain fermentation broth and a compound microbial agent have significant promotion effects on plant heights, root lengths and fresh weights of four selected crops; and after the root is irrigated with the complex microbial inoculant for 15 days, the control effect on the soybean root rot caused by fusarium oxysporum reaches 73.69%. The results show that the Penicillium spiniferum LS001 and the Trichoderma asperellum LS002 are disease-preventing and growth-promoting microbial strains with good effects, and the microbial agent formed by compounding the Penicillium spiniferum LS001 and the Trichoderma asperellum LS002 has efficient disease-preventing and growth-promoting effects on leguminous crops.

The application discloses a method for controlling ear diseases and pests of corn by using insecticidal diatomaceous earth, green muscadine and wood fungus in combination; the insecticidal diatomaceous earth, the dispersible oil suspension agent of the green muscadine beetle CQMa421 and / or the spore suspension of the echinoporus GDFS1009 are combined to be sprayed on the body of the corn borer larvae or the ears or leaves infected by the corn borer and the ear rot fungus, the control effect on the corn borer reaches 60-80%, and the control effect on the ear rot caused by the pseudorhizomycete fusarium reaches 60-100%. It is found that the control effects on the corn borer larvae and the ear rot are obviously higher than those of the same amount of single preparation, the synergistic green prevention and control of the ear corn borer and the ear rot is realized, and it is a new green prevention and control technology for the ear diseases and pests of corn.

The present application provides a strain of Trichoderma asperellum TAZ19, a biocontrol agent and application thereof, wherein the Trichoderma asperellum TAZ19 is preserved in China Center for Type Culture Collection on October 25, 2024, and the preservation number is CCTCC M 20242341. The active ingredient of the biocontrol agent comprises the above-mentioned Trichoderma asperellum TAZ19 strain, and the Trichoderma asperellum TAZ19 strain is one or more of conidium, mycelium, fermentation filtrate or active extract thereof. The Trichoderma asperellum TAZ19 has excellent antagonistic effect on multiple plant disease pathogens such as Diaporthe phaseolorum, multiple Fusarium oxysporum, Valsa mali, and Botryosphaeria dothidea, has broad-spectrum antibacterial property, fast growth, high antibacterial activity, strong parasitism, and good salt and alkali tolerance, and has a growth-promoting effect on plants. The Trichoderma asperellum TAZ19 can be widely applied to the prevention and treatment of multiple plant diseases such as pine branch and stem rot, wheat stem base rot, tomato wilt, and has a wide application prospect.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for saline-alkali soil and a biological bacterial fertilizer of the micro-ecological restoration liquid composite microbial agent. The micro-ecological restoration liquid complex microbial inoculant suitable for the saline-alkali soil is prepared from restoration salt-tolerant bacteria, namely bacillus subtilis, nocardia halospora, nitrogen-fixing bacteria, namely azotobacter chroococcum, phosphate solubilizing bacteria, namely pseudomonas fluorescens, potassium solubilizing bacteria, namely bacillus mucilaginosus, growth-promoting bacteria, namely azospirillum brasilense, biocontrol bacteria, namely bacillus megatherium and synergistic bacteria, wherein the restoration salt-tolerant bacteria are bacillus subtilis, nocardia halospora, the nitrogen-fixing bacteria are azotobacter chroococcum, the phosphate solubilizing bacteria are pseudomonas fluorescens, the potassium solubilizing bacteria are bacillus mucilaginosus, the growth-promoting bacteria are azospirillum brasilense, the biocontrol bacteria are bacillus megatherium, and the synergistic bacteria are prepared from bacillus subtilis The biological bacterial fertilizer for the saline-alkali soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the saline-alkali soil is greatly improved, the saline-alkali soil is effectively improved, and the crop yield is remarkably increased.

The application discloses a kind of fluorine-resistant trichoderma asperellum and the fungicide prepared by it, belong to microbial strain and application technical field.The strain is named Trichoderma asperellum M700, has been preserved in China typical culture preservation center (CCTC), and its preservation number is CCTCC NO: M 2024579, and the preservation date is March 27, 2024.The beneficial effect: the trichoderma asperellum strain of the application Trichoderma asperellum. Colony growth early stage presents white mycelium, later generation green spore, causes the body to present yellow green, green, aerobic, can survive at ph 3.0-10.0, temperature is 25-28 DEG C.The strain has very good resistance, and can grow when the fluorine ion concentration is below 800 mg / L.Can very good dispel fluorine element in water body.

The invention discloses a microbial preparation and a preparation method and application thereof, the microbial preparation has active ingredients of trichoderma koningii Tr25 and trichoderma asperellum Tr85, the classification name of trichoderma koningii Tr25 is Trichoderma koningii Tr25 is Trichoderma koningii, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) 7.284; the classification name of the trichoderma asperellum Tr85 is Trichoderma asperellum, and the preservation number of the trichoderma asperellum Tr85 is CGMCC (China General Microbiological Culture Collection Center) 7.283. The trichoderma koningii Tr25 has a good nutrition space-occupying resistance induction effect, and the trichoderma asperellum Tr477 has good antagonism and hyperparasitism effects; trichoderma koningii Tr25 and Trichoderma asperellum Tr477 belong to the same trichoderma, and show good compatibility compared with the combination of different biocontrol bacteria, and the combination of the two strains has a good control effect on the pathogenic bacteria of the sorghum leaf spot disease.

The application discloses a method for synthesizing lactic acid from lignocellulose by using a fungus-bacterium mixed culture system with functional complementation, and comprises the following steps: (1) inoculating activated Trichoderma asperellum into a fermentation medium containing microcrystalline cellulose to perform fermentation, and obtaining a fermentation liquor; and (2) inoculating activated Lactobacillus paracasei into the fermentation liquor obtained in the step (1) to perform anaerobic fermentation and produce lactic acid. When the Lactobacillus paracasei is inoculated when the Trichoderma asperellum is cultured for 40 hours, the highest lactic acid yield is achieved, and reaches 57.59 g / L. The method is currently used to obtain the highest lactic acid yield in the mixed culture fermentation by using microcrystalline cellulose as the only carbon source. Meanwhile, the method can also be applied to a fermentation process for directly synthesizing lactic acid from lignocellulose. When the fermentation medium with corn cobs as the substrate is used to perform fermentation, 9.80 g / L of lactic acid can be produced, the substrate cost for industrial production of lactic acid is greatly reduced, and the method has important application value.