182 results about "Trichoderma asperellum" patented technology

The invention discloses a trichoderma spore powder as well as a preparation method and application thereof. The trichoderma spore powder contains trichoderma spore powder and a drying aid, wherein the trichoderma spore powder is the spore powder of Trichoderma sperellum T1 which is preserved in the general microorganism center of CCCCM (China Committee for Culture Collection of Microorganisms) and has the preservation number of CGMCC NO: 3531 and a rDNA ITS region segment sequence shown in SEQ ID NO:1; the drying aid is one or more of beta-cyclodextrin, potato starch and soluble starch. The trichoderma spore powder has an effective prevention and control effect on banana vasicular wilt, and has a remarkable antagonism effect on various pathogenic funguses, especially on soil-borne funguses. The preparation time is saved with the preparation method of the trichoderma spore powder, which can be used for preparing the trichoderma spore powder which has high density, high germination rateand high drying efficiency and is easy to store, transport and use.

The invention belongs to the technical field of microorganisms, and particularly relates to a biocontrol strain, namely trichoderma asperellum T-1, for highly producing composite cellulose degrading enzyme. The trichoderma asperellum T-1 is preserved at the China General Microbiological Culture Collection Center on September 24, 2014; and the preservation number is CGMCC 9722. The invention also provides a waste composting bactericide prepared from the trichoderma asperellum T-1 and an application of the waste composting bactericide in treatment of the organic waste. The trichoderma asperellum T-1 provided by the invention is rapid in growth, high in spore-producing ability, and high pH tolerance; a plurality of cellulose materials can be taken as substrates; and the trichoderma asperellum T-1 is a member of biocontrol bacteria, and can be applied to planting of crops to prevent diseases. By adding the trichoderma asperellum T-1 provided by the invention to the organic waste, the penetrating fluid generated by waste composting can be reduced; the degradation efficiency of the cellulose materials in the waste can be improved; and the quality of the waste composting product is improved.

The invention discloses a trichoderma asperellum strain and application thereof. The trichoderma asperellum strain is named as trichoderma asperellum Thz01, and the preservation number of the trichoderma asperellum strain is CGMCC (China general microbiological culture collection center) No.6422. The trichoderma asperellum strain is separated from a capsicum habitat, has a remarkable antagonism effect to phytophthora capsici, can be obviously subjected to hyperparasitism on the phytophthora capsici, an can effectively degrade mycelia of the phytophthora capsici, and a biocontrol agent prepared from the strain can be used for effectively preventing and treating phytophthora capsici leonian .

The present invention relates to a single-cell protein biological feed and a preparation method thereof. Particularly, the single-cell protein biological feed is prepared from rhizoma atractylodis medicinal residues and prepared by a two-step fermentation method using shiraia bambusicola, and trichoderma asperellum and candida utilis. The feed has effects of improving an egg laying rate of layingchickens, enhancing immunity, etc.

The invention relates to trichoderma asperellum and an application thereof in remediation for heavy metal pollution. The trichoderma asperellum HD228 is separated from cadmium-containing soil in Hengdong County, Hunan, and the preservation number of the trichoderma asperellum HD228 is CGMCC No.12870. The strain (the trichoderma asperellum HD228) has a high cadmium adsorbing capacity, and meanwhile, the strain has a growth promoting effect on rice and has a detoxifying effect on cadmium-stressed rice. Therefore, the trichoderma asperellum HD228 has a potential of developing double functions in a process of conducting soil remediation on cadmium-contaminated rice fields, and a good strain is provided for cadmium-contaminated soil microbial remediation engineering. The application provided by the invention has theoretical and realistic significance for the remediation of cadmium pollution of the cadmium-contaminated rice fields.

The invention relates to a solid fermentation production method of trichoderma asperellum. The trichoderma asperellum bacterial strain used in the method is preserved in the general microbiological center of Chinese general microorganism strain preservation management committee, the preservation date is October 27th, 2015, and the preservation number is CGMCC No.11528. The trichoderma asperellum is subjected to strain activation, shake flask seed preparation, primary liquid seed culture and then solid fermentation. A solid fermentation culture medium for solid fermentation is composed of rice husks, corn straw powder, wheat bran or corn powder and inorganic salt. According to the solid fermentation method of the trichoderma asperellum, a large quantity of conidia can be generated, the spore number of the substrate trichoderma asperellum can reach up to 1*109-1*1010 cfu/g, the fermentation viable count is high, neither pollution nor waste materials are generated in the production process, and the efficiency of the production technology is high. Fermentation products obtained after fermentation have obvious growth-promoting effects on crops and greatly improve the economic benefit of the crops.

The invention relates to a Trichoderma strain for antagonizing soil-borne disease, belonging to the field of biological control. The Trichoderma strain is Trichoderma asperellum ZJSX5003 CGMCC No.6480. The Trichoderma strain provided by the invention has the characteristics of high growth speed and high spore production amount; the in-vitro standoff pathogen inhibition rates are respectively up to 79.79%, 73.68% and 72.25%, the pathogen spore survival rates are respectively 0.00%, the in-vivo preventive effects for cucumber fusarium wilt and maize stem rot are respectively 85.08% and 66.67%, the enzyme activity of chitinase is 4.0783U, the enzyme activity of beta-1,3-glucanase is 0.8050U, the enzyme activity of extracellular proteinase is 4.8503U, and the content of silicone, terpene and carboxylic acid having antagonistic actions in antibiotic secondary metabolites is 44.44%. The Trichoderma strain provided by the invention has the advantages of high antagonism and high specificity. The biological pesticide produced from the strain can effectively control the soil-borne disease, thereby ensuring the stable increase of crop and vegetable yields.

The invention discloses a Trichoderma asperellum TC01. The Trichoderma asperellum TC01 is collected in the China General Microbiological Culture Collection Center, and the collection number is CGMCC NO. 3.19218. The Trichoderma asperellum TC01 has the remarkable inhibiting effect on a tea-tree anthracnose pathogenic bacterium Colletotrichum gloeosporioides and an apple valsa canker pathogenic bacterium Phytophthora plurivora, and antibacterial substances generated by the Trichoderma asperellum TC01 can also inhibit growth of the tea-tree anthracnose pathogenic bacterium and the apple valsa canker pathogenic bacterium. The trichoderma asperellum can also promote growth of tea seedlings, and is a strain having the good environmental safety, the biological control capacity and the growth promotion function.

The invention belongs to the technical field of pesticides with microbial activity. A trichoderma pill provided by the invention comprises a trichoderma spore suspension and a pill matrix, wherein the trichoderma T1 bacterial strain spore suspension is prepared from spore powder of trichoderma asperellum T1 which is preserved in an ordinary microorganism center of the CCCCM (China Committee for Culture Collection of Microorganisms), wherein the preservation number is CGMCC (China General Microbiological Culture Collection) NO: 3531 and the preservation date is December 17, 2009; the fragment sequence in DNA ITS region is shown as SEQ ID NO:1; a preservative agent of a spore suspension with the concentration of 0.5-1.5g/l is contained in the invention; and the pill matrix is a mixture of one or two or three of polyethylene glycol 1000, polyethylene glycol 2000 and polyethylene glycol 4000. The trichoderma pill provided by the invention has the advantages of high dose content, high biological activity and good molding effect, can be dissolved in the shortest time, and is convenient for storage, transportation and use.

The invention discloses a method for preparing (R)-[3,5-bi(trifluoromethyl) phenyl]ethanol in a reaction system containing ionic liquid. The method comprises the following steps: with wet mycelium obtained by cultivation of trichoderma asperellum ZJPH0810 as a catalyst and 3,5-bi(trifluoromethyl) hypnone as a substrate, reacting for 6-50 hours under the conditions of 25-45 DEG C and 100-300r/min inside the reaction system composed of amino acid ionic liquid shown in a formula (I) and a phosphate buffer of which the pH value is 5.5-8.0; and separating and purifying the reaction liquid to obtain the product (R)-[3,5-bi(trifluoromethyl) phenyl]ethanol. The amino acid ionic liquid disclosed by the invention has the function of facilitating coenzyme regeneration, and is low in toxicity, green and environment-friendly, renewable and biodegradable; the reaction system containing the ionic liquid is applied to biological catalytic reaction, so that the method is friendly to the environment, and accords with the green development strategy, and the defects that the traditional ionic liquid is poor in biodegradability, nonrenewable and the like can be overcome.

The invention relates to trichoderma asperellum chlamydospore powder as well as a preparation method thereof and an application. The preparation method of the trichoderma asperellum chlamydospore powder comprises the following steps: conservatively culturing arsenic-resisting activity, purifying, culturing shake-flask seeds, culturing fermented seeds, controlling the fermentation and post-processing. The trichoderma asperellum chlamydospore is high in adversity resistance, the powder of the trichoderma asperellum chlamydospore is slightly subjected to the ambient temperature, acidicity, alkalinity and the like, and the influence of different arsenic concentrations in the environment on the sprouting of the chlamydospore is not obvious. The trichoderma asperellum chlamydospore has strong vitality, the survival time of the trichoderma asperellum chlamydospore is long, the volatilization of arsenic in the soil can be obviously promoted, the total quantity of the arsenic in the soil can be reduced, and the soil polluted by the arsenic can be effectively repaired. By adopting the method, the time for producing a great amount of trichoderma asperellum chlamydospore can be greatly shortened, the production cost of the trichoderma asperellum chlamydospore can be remarkably reduced, and the popularization of the technology can be favored.

The invention relates to a dispersible trichoderma asperellum oil suspending agent and a preparation method thereof, belonging to the technical field of a suspending fungicide and aiming at solving the technical problem that existing trichoderma asperellum cannot be stored for a long time. The dispersible trichoderma asperellum oil suspending agent comprises trichoderma asperellum conidia powder, an emulsifier, a stabilizer, a wetting dispersant, an antioxidant, a thickener, an ultraviolet protective agent, a preservative and plant oil. The dispersible trichoderma asperellum oil suspending agent is prepared by the following concrete steps: (I) adding the emulsifier, stabilizer, wetting dispersant, antioxidant, thickener, ultraviolet protective agent and preservative into the plant oil, premixing through a magnetic stirrer, and then processing for 10min-30min in a homogenizer or a high-speed shearing machine to obtain a mixture; and (II) mixing the mixture with the trichoderma asperellum conidia powder at room temperature, and grinding for 10-60 minutes. The dispersible trichoderma asperellum oil suspending agent is less in usage amount, not only is suitable for disease control of tall trees of forests in high mountains with drought, but also has the characteristics of being environmentally friendly, free from resistance, harmless to human and livestock and the like.

The invention relates to aqueous dispersible granules of viable microbe biopesticide taking trichoderma asperellum chlamydospores as a main component, and a preparation method of the aqueous dispersible granules of viable microbe biopesticide, belonging to the technical field of biopesticide and biochemistry. The preparation method comprises the following steps of: cultivating high-concentration trichoderma asperellum chlamydospores by using a culture medium with efficient composites in a liquid fermentation manner; mixing trichoderma asperellum chlamydospores serving as an active component with a microbial thallus protective agent, a disintegration agent and an adhesive; and pelletizing and drying to finally obtain the aqueous dispersible granules of biopesticide. The aqueous dispersible granules of biopesticide has high content of trichoderma asperellum chlamydospores and strong storage property and ultraviolent resistances, and can disintegrate and disperse quickly after being dissolving in water; the dust flying during the use process is avoided; and the aqueous dispersible granules of biopesticide have the advantages of wettable powder and aqueous suspending agents, so the stability of the microbial pesticide preparations in prevention and control of plant diseases is improved greatly.

The invention provides efficient biocontrol trichoderma asperellum for tobacco fusarium root rot and application thereof, the preservation name of the trichoderma asperellum is trichoderma asperellum Tr-0111, the trichoderma asperellum is preserved in the China Center for Type Culture Collection, the preservation date is August 21, 2020, and the preservation number is CCTCC NO: M 2020441; according to the research, soil microorganisms are separated and screened to obtain an efficient biocontrol fungus strain with a relatively good inhibiting effect on tobacco fusarium root rot, the variety of the strain is determined by utilizing a method of combining traditional morphology and modern molecular biology, and the growth promoting effect of the strain on tobacco is determined; a foundation is laid for further development of research on the control effect and bacteriostatic active substances of efficient biocontrol bacteria potted plants and productization and industrial production of biocontrol bacteria, and the efficient biocontrol trichoderma asperellum has certain scientific basis and application value for biological control of soil-borne diseases in main tobacco-growing areas in Henan province.

The invention discloses a method for screening trichoderma asperellum and application thereof. The method comprises the following steps of: collecting an arsenic pollution soil sample; separating fungi from the soil sample in a laboratory; purifying the fungi, and transferring the purified fungi to a selective culture medium of trichoderma; and culturing the fungi in different arsenic concentrations by taking a fungus strain of which the growth condition is the best on the selective culture medium as an object, and observing the growth condition of a bacterial colony and the tolerance of the bacterial colony on arsenic to screen the trichoderma asperellum.

The invention provides Trichoderma asperellum TD3104 and application thereof in preparation of microbial agent for inhibiting plant pathogenic bacteria. The preservation number of the Trichoderma asperellum TD3104 is CGMCC 13161. The invention further provides application of the Trichoderma asperellum in the controlling of the plant pathogenic bacteria of tobacco and apples. The antagonistic Trichoderma asperellum can effectively inhibit the growth of Phytophthora parasitica var. nicotianae, ralstonia solannacearum, rhizoctonia solani, tobacco root rot pathogenic fusarium, alternaria alternata and the pathogenic bacteria of apple rot and apple ring rot, can effectively control related diseases, can reduce the use amount of chemical pesticides and accumulation of the chemical pesticides in soil, is beneficial to the increasing of quality and yield of the economic crops of China and is evident in economic and social benefits.

The invention discloses a functional biochar-based nutrition medium for preventing and treating a root rot and a preparation method. The functional biochar-based nutrition medium is characterized by being prepared from the following components in parts by weight: 10-30 parts of a forestry and agricultural waste carbonized material, 0.1-1 part of trichoderma asperellum CGM-10 conidial powder of which the preservation number is CCTCC No. M 2014001, 10-20 parts of a trametes trogii SEM-6 bacteria solution of which the preservation number is CCTCC No. M 2014002, 0-10 parts of humic acid, 0-50 parts of municipal dry sludge, 0-50 parts of dry biogas residue and 0-20 parts of plant ash; and the components are mixed evenly and baked until the moisture content is 20-30% to obtain the functional biochar-based nutrition medium for preventing and treating the root rot. The functional biochar-based nutrition medium has the effects of obviously promoting plant growth and improving the performance of soil, can be used as a fertilizer, a nutrient medium, nutrient soil and the like, and has wide application value in the aspect of agricultural crop planting.

The invention relates to the field of biopesticide, and in particular to a Trichoderma asperellum spore wettable powder composition and a preparation method thereof. The wettable powder composition consists of the following components: 10-55% of Trichoderma asperellum spore powder, 2-15% of a wetting agent, 2-8% of a dispersant, 6-10% of a stabilizer and the balance of a carrier. The Trichoderma asperellum spore powder is subjected to heat storage culture and screening, and is cultured to at least three generations and bred. The spore powder has a higher survival rate at high temperature and under heat storage, so that the product has a long shelf life, can be stored for a long time at a predetermined temperature, and has a long persistence period under high temperature application conditions.

The invention relates to a rapid returning method for production of bacterial manure from straws. The method comprises the steps: inoculating a liquid strain fermentation tank containing a fermentation medium with a spore aqueous solution of trichoderma asperellum strain T-A158 to ferment for 7-10 days to obtain a trichoderma asperellum fermentation liquid; mixing ferrous sulfate, ammonium molybdate, and the trichoderma asperellum fermentation liquid to prepare a trichoderma asperellum straw decomposed solution; spraying the trichoderma asperellum straw decomposed solution containing spores 1is multiplied by 10<7> spores/mL on straws and humic acid; the dosage being 30-40L per mu, spraying water and evenly arranging a layer of straws on the top, the thickness being 7-12 cm, making the final moisture content be 40-46 percent; covering with a mulch and fermenting at room temperature for 7-20 days; then directly plowing the land for cultication. The rapid returning method provided by theinvention can effectively alleviate the occurrence of pests and diseases in a greenhouse, increase the soil fertility in the greenhouse, solve the problem of disadvantages of nowhere to place the straws and returning the straws to the field, reduce the use of the fertilizer, increase the yield of the crop, improve the quality of the crop, and improve the soil environment.

The invention provides a compound fungicide comprising Trichoderma asperellum spore powder of and application thereof. The fungicide comprises the following components byweight: aculeatus, 2-30% of Trichoderma asperellum spore powder, 5-40% of a chemical fungicide, 5-10% of a surfactant and the balance of a carrier. The chemical fungicide is one of phenamacril, picoxystrobin, dimethomorph, cyazofamid, fluopicolide, hymexazol, propamocarb hydrochloride and thifluzamide; and the a surfactant comprises a dispersant and an active agent. The fungicide of the invention has the advantages of quick result, long duration and small amount, can effectively control soil-borne diseases of crops; and chemical fungicide and the surfactant in the product are repeatedly screened and have little influence on the activity of Trichoderma asperellum spore powder.