74 results about "Trichoderma asperellum" patented technology

The invention discloses preparation of a trichoderma composite flora and application of the trichoderma composite flora in prevention and control of crop fusarium wilt and improvement of soil, and relates to the field of agricultural production, and the composite flora is prepared by mixing trichoderma asperellum (5 * 10 < 7 > CFU / L), streptomyces (108 CFU / L), Chinese monospora (108 CFU / L), ensifer adhesion (108 CFU / L), pseudomonas nitroreducens (108 CFU / L) and paenibacillus alvei (108 CFU / L) according to the volume ratio of 6: 1: 1: 1: 1: 1: 1: 1 (V: V). According to the composite flora in the scheme, the composite flora is used for promoting growth of cucumber plants, and compared with a clear water control group (Foc), the fresh weight of cucumbers is increased by 55.51%, the stem diameter is increased by 12.76%, and the plant height is increased by 34.25%. The composite flora can also effectively prevent and treat fusarium wilt of cucumbers, and the prevention and treatment effect reaches 71.43%. Compared with the Trichoderma asperellum FJ035 treated group, the Trichoderma asperellum FJ035 has the advantages that the Trichoderma asperellum FJ035 is better than the Trichoderma asperellum FJ035 which is independently applied in the aspects of promoting the growth of cucumber plants and preventing and treating cucumber fusarium wilt, and compared with the Trichoderma asperellum FJ035 treated group, the control effect is improved by 56.25%. Meanwhile, the composite flora can be used for improving soil and preventing and treating multi-crop fusarium wilt.

The invention discloses trichoderma asperellum TS7-1 capable of efficiently degrading macrolide antibiotics and application of the trichoderma asperellum TS7-1, and belongs to the technical field of microorganisms. The preservation number of the trichoderma asperellum TS7-1 is CGMCC (China General Microbiological Culture Collection Center) No. 41372. The Trichoderma asperellum TS7-1 (CGMCC No.41372) provided by the invention has high-efficiency degradation capability on various macrolide antibiotics (erythromycin, roxithromycin and azithromycin), the removal rates of the Trichoderma asperellum TS7-1, roxithromycin and azithromycin in 36h all reach 85% or above, and the Trichoderma asperellum TS7-1 has high-concentration antibiotic tolerance, degradation rate and degradation spectrum obviously superior to those of other strains in the prior art. The trichoderma asperellum TS7-1 is directly separated from a natural soil body which is not polluted by antibiotics, has no animal and plant pathogenicity hidden danger, has no microbial toxicity to degradation products of macrolide antibiotics, avoids secondary pollution, and has excellent environmental safety.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention discloses trichoderma asperellum TR35 and application thereof in inhibition of mulberry bacterial wilt and prevention and treatment of mulberry bacterial wilt, the trichoderma asperellum TR35 is identified as Trichoderma asperellum, the trichoderma asperellum TR35 is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.64886, and the preservation time is July 18, 2024. The trichoderma asperellum TR35 obtained through separation and screening has good inhibition and antagonism effects on mulberry ralstonia solanacearum, and can be used for preventing and treating mulberry bacterial wilt. The inhibition rate of the trichoderma asperellum TR35 to the mulberry ralstonia solanacearum reaches up to 98.25%, and the active fermentation liquor of the trichoderma asperellum TR35 not only can significantly inhibit the growth of the ralstonia solanacearum and reduce the forming ability of a biological membrane, but also can destroy the integrity and continuity of the biological membrane of the ralstonia solanacearum. In addition, the bacterial zone of the ralstonia solanacearum can be reduced, the athletic ability is reduced, and the expression of virulence-related genes in the ralstonia solanacearum is remarkably inhibited.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention relates to the technical field of agricultural microorganisms, in particular to trichoderma asperellum and application thereof. The Trichoderma asperellum disclosed by the invention is preserved in the China Center for Type Culture Collection on October 25, 2023, and the preservation number of the Trichoderma asperellum is CCTCC (China Center for Type Culture Collection) NO: M20232021. The trichoderma asperellum provided by the invention has a relatively strong inhibition effect on onion specialized fusarium oxysporum, and has a good prevention and treatment effect on onion root rot; the fertilizer has strong salt and alkali resistance, can promote growth of onions under a salinization condition, improve growth traits and fruit quality of the onions, increase the yield of the onions, improve physicochemical properties of salinized soil and change abundance of microflora of the soil; in addition, the disinfectant is safe and convenient to use, non-toxic, harmless, non-corrosive, harmless to human, livestock, animals and plants, free of environmental pollution and ecological in safety.

The invention belongs to the technical field of environmental protection, and relates to screening, domestication and application of focused polyacrylamide (PAM) for efficiently degrading fungi. The strain (# 12) is preserved in CGMCC (China General Microbiological Culture Collection Center) and is trichoderma asperellum, and the similarity between the ITS sequence of the strain and the gene reaches 99%. Aiming at soil hardening and acrylamide pollution caused by difficult degradation of non-ionic PAM in bauxite slime, the inventor adopts a gradient concentration screening and multi-stage domestication process. The method comprises the following steps: carrying out enrichment culture on slurry containing PAM, carrying out gradient screening in a basic solution with a specific concentration, separating and purifying to obtain an optimal strain, and carrying out continuous passage domestication through a six-stage culture medium, so that the degradation rate is increased by 23.45%. Under optimized conditions, the PAM degradation rate of the strain reaches 64.18%, the viscosity is reduced by 88.03%, degradation is achieved through physical destruction and enzymatic hydrolysis, and no acrylamide monomer is detected. The strain has activity at the temperature of 25-45 DEG C and the pH value of 4-8, is suitable for bioremediation of PAM-containing slime, alleviation of soil hardening and elimination of risks of toxic and side products, reveals the efficient degradation characteristic of trichoderma fungi to nonionic PAM for the first time, and assists in recycling of mining wastes.

The invention relates to the technical field of agricultural microorganisms, in particular to trichoderma asperellum and application thereof.The trichoderma asperellum comprises at least one of the following trichoderma asperellum TA727, the trichoderma asperellum TA727 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO. 63859. The trichoderma asperellum has the advantages that the trichoderma asperellum can be used for preparing the trichoderma asperellum; the trichoderma asperellum is high in sporulation quantity and strong in acid resistance, and has a relatively good pathogenic bacterium inhibition effect under an acidic condition, and volatile substances of the trichoderma asperellum also have an antibacterial effect. The trichoderma asperellum can effectively inhibit pathogenic bacteria in an acid soil environment and soil-borne diseases caused by the pathogenic bacteria, meanwhile, industrial production can be facilitated, and the trichoderma asperellum has wide application prospects.

The invention discloses a composite microcapsule of bacillus subtilis and trichoderma asperellum, which is prepared by the following steps: embedding bacillus subtilis Pn1 and trichoderma asperellum Ta1 by adopting sodium alginate, and carrying out microencapsulation treatment, so that the controllable release of the bacillus subtilis Pn1 and the trichoderma asperellum Ta1 is realized; therefore, the stability of the composite biocontrol bacterium under adverse environmental factors is improved, meanwhile, the inhibition effect on root rot bacteria is enhanced, and the sodium alginate embedded bacillus subtilis Pn1 and trichoderma asperellum Ta1 have important application value in prevention and treatment of panax notoginseng biological root rot.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The invention provides bacillus cereus, the bacillus cereus is named as bacillus cereus, the bacillus cereus is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 66395. The invention further provides a synthetic flora, and the synthetic flora contains the bacillus cereus and the trichoderma asperellum MM7. The invention further provides a preparation method of the bacillus cereus and the trichoderma asperellum MM7. A synthetic flora constructed by the bacillus cereus and Trichoderma asperellum MM7 has a better effect of promoting the growth of banana plants, can be used for preparing a mixed bacterial agent or a microbial fertilizer, is beneficial to improving the economic benefits of the banana planting industry, and has a wide application space in green and sustainable development of the banana industry.

The invention relates to the technical field of new pollutant degradation, and particularly discloses a method for improving the dibutyl phthalate degradation efficiency of microorganisms by using biochar. The method comprises the following steps: fermenting straw powder by using a trichoderma asperellum T-1 spore suspension, performing pyrolysis carbonization on fermented straw residues to obtain biochar, and adding the biochar into a pollutant solution containing degrading bacteria for reaction. When the method is applied to wastewater, dibutyl phthalate pollution in water can be efficiently removed, the negative influence of dibutyl phthalate on the environment is reduced, and the water quality is improved. According to the method, the microbial degradation efficiency can be improved by 44.8%, and the method has a good application prospect.

The invention discloses a trichoderma complex microbial inoculant capable of promoting wheat growth and preventing and treating wheat stem rot and application of the trichoderma complex microbial inoculant. The trichoderma complex microbial inoculant comprises a trichoderma asperellum strain XX-57 and a trichoderma harzianum strain PY-127, and the preservation number of the trichoderma asperellum strain XX-57 is CGMCC No. 41937; the preservation number of the trichoderma harzianum strain PY-127 is CGMCC (China General Microbiological Culture Collection Center) NO. 41938. The invention further specifically discloses application of the trichoderma complex microbial inoculant in promoting wheat seed germination and wheat growth and preventing and treating wheat basal stem rot, the trichoderma complex microbial inoculant is applied 10-20 days after wheat seedling emergence, and it is found that compared with single trichoderma inoculation, the trichoderma complex microbial inoculant has the promoting effect on wheat plant height, root length, SPAD value, fresh weight and dry weight; the control effect on wheat stem rot is better, and particularly the control effect is more obvious when pathogenic bacteria are inoculated in advance.

The application discloses application of Trichoderma asperellum strain YN4 and fungicides in prevention and treatment of plant fungal diseases. The application research shows that the Trichoderma asperellum strain YN4 has a good inhibitory effect on pepper anthracnose, rubber anthracnose, mango anthracnose, soybean anthracnose and the like. Further, compared with the single use of fungicides or the Trichoderma asperellum strain, the inhibitory effect of the combination of the two is better. Under certain conditions, the Trichoderma asperellum strain is combined with 10% difenconazole water dispersible granules, 70% thiophanate-methyl wettable powder or 450g / L prochloraz emulsion in water, and has a synergistic effect. Not only can the use amount of fungicides be greatly reduced, but also the inhibitory effect on plant pathogenic fungi can be further improved, the incidence can be reduced, the inhibition on pathogenic bacteria can be enhanced, the use and residue of chemical pesticides can be reduced, and technical support is provided for the selection of fungicides for pepper anthracnose and the like and the combination of bacteria and drugs.

The invention discloses an efficient detoxification and nutrient recovery method for aerobic composting of fungus-enhanced erythromycin fermentation fungus residues, and belongs to the field of solid waste disposal and resource reutilization. The method comprises the following steps: mixing erythromycin fermentation mushroom dregs subjected to high-temperature sterilization treatment with corn straw powder, inoculating trichoderma asperellum, and carrying out aerobic composting fermentation. According to the method, aerobic co-compost of erythromycin fermentation mushroom dregs and crop straws is strengthened by inoculating an antibiotic degrading microbial agent taking trichoderma asperellum as a core, and an organic fertilizer which is high in nutrient content and is non-toxic and harmless is produced, so that an efficient, economical and environment-friendly solution is provided for solving the problem of resource utilization of the erythromycin fermentation mushroom dregs.

The invention relates to the field of environmental pollution treatment and microorganisms, and discloses a preparation method of a trichoderma asperellum straw fermentation extracting solution and application of the trichoderma asperellum straw fermentation extracting solution in oxytetracycline pollution remediation or crop growth promotion. The method comprises the following steps: 1) performing solid-state fermentation on a trichoderma asperellum T1 seed solution to obtain a fermentation product; and 2) drying the fermentation product, adding the dried fermentation product into the leaching solution for leaching, and filtering to obtain the trichoderma asperellum straw fermentation extracting solution rich in trichoderma asperellum spores, enzyme and reducing sugar. The invention finds that the trichoderma asperellum T-1 has new application of repairing oxytetracycline pollution and promoting crop growth, can efficiently degrade oxytetracycline at normal temperature especially in the aspect of repairing oxytetracycline pollution, and has practical application value. Furthermore, compared with a non-extracted trichoderma asperellum leavening and pure trichoderma spores, the trichoderma asperellum straw fermentation extracting solution obtained by a specific process has a better oxytetracycline pollution repairing effect and a better crop growth promoting effect respectively in comparison with the non-extracted trichoderma asperellum leavening and the pure trichoderma spores.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

The present invention discloses Trichoderma asperellum ZC3 and its application in the degradation of Zanthoxylum bungeanum branches. This strain has a fast growth rate and strong degradation ability. In a liquid filter paper strip culture medium, after culturing at 28 °C for 7 days, the filter paper strip completely disintegrates; in a solid culture medium of Zanthoxylum bungeanum branch powder, after culturing at 28 °C for 40 days, its lignin degradation rate for Zanthoxylum bungeanum branches reaches 78.11%, and the degradation rates of cellulose and hemicellulose reach 82.58% and 75.84% respectively. Under a scanning electron microscope, obvious fiber breakage and structural disintegration phenomena can be seen in the Zanthoxylum bungeanum branches, showing a high degradation ability. In addition, the lignin-degrading bacterium ZC3 has good tolerance to the leaching solution of Zanthoxylum bungeanum branches.

The invention relates to a composition with sleep aiding and skin repairing effects and a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a composition with sleep aiding and skin repairing effects. The composition is prepared from the following components in parts by weight: 72 to 114 parts of compound plant essential oil, 3 to 5 parts of ceramide NP and 1 to 3 parts of sodium hyaluronate, the compound plant essential oil is prepared from lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil and rhodiola rosea essential oil; the rhodiola rosea essential oil is obtained by fermenting a mixed strain composed of kluyveromyces lactis and trichoderma asperellum and then extracting essential oil. The research shows that the mixed strain fermentation can synergistically improve the extraction rate of the rhodiola rosea essential oil. In addition, the compound plant essential oil provided by the invention can also synergistically improve the anti-inflammatory, moisturizing and anti-allergy repair capabilities of the composition and has the effect of improving sleep disorders, so that the composition has better sleep aiding and skin repairing effects at the same time.

The invention relates to the technical field of extraction of green pesticides from natural products of trichoderma asperellum, relates to an alkylated citrate compound, relates to application of the alkylated citrate compound in preparation of drugs for preventing and treating plant diseases, and further relates to application of the alkylated citrate compound in preparation of drugs for preventing and treating plant diseases by using a computer deep learning model and combining with pathogenic proteins of phytopathogens. And predicting the antibacterial activity of the alkylated citrate compound.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention discloses an iron-based biological leaf surface protective agent as well as a preparation method and application thereof, and the iron-based biological leaf surface protective agent is prepared from the following raw materials in parts by weight: 2 to 20 parts of EDTA-Fe, 50 to 200 parts of urea, 30 to 100 parts of monopotassium phosphate, 10 to 20 parts of boric acid, 20 to 40 parts of magnesium sulfate, 200 to 400 parts of nano silicon dioxide, 30 to 100 parts of a biological source synergist and 11 to 40 parts of an auxiliary agent, the biological source synergist comprises a Trichoderma asperellum TaLY2 fermentation broth, the Trichoderma asperellum TaLY2 fermentation broth is obtained by inoculating a Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum The iron-based biological leaf surface protective agent disclosed by the invention solves the problems of short effective period, high application frequency and single function of a traditional leaf surface iron fertilizer, has a good prevention and treatment effect on the curvularia leaf spot of corn, further promotes the growth of the corn and improves the yield of the corn.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

The invention relates to a trichoderma asperellum sourced Cerata-plaatanin (CP) family protein CP3 and application thereof, and belongs to the technical field of biological prevention and control of plant diseases. The CP3 protein is derived from Trichoderma asperellum, belongs to a Cerato-platanin family and has a conservative CP structural domain, the amino acid sequence registration number of the CP3 protein is XP024756165.1, and the registration number of a coding gene of the CP3 protein is 36612810 (Gene ID). The protein is a secreting type protein, has signal peptide and can be positioned in tomato cell nucleuses. The invention proves that the disease spot area of tomatoes infected with Botrytis cinerea can be reduced by 71% after the CP3 protein is exogenously applied; after tomatoes are treated by the trichoderma asperellum CP3 overexpression strain, the area of gray mold scabs is remarkably reduced, and the CP3 gene knockout strain has no disease-resistant effect. The CP3 protein and the trichoderma asperellum strain expressing the CP3 protein can be used for preparing biological agents for enhancing the botrytis cinerea resistance, application modes comprise rhizosphere application, root system inoculation and the like, adverse effects on tomato growth are avoided, and a new effective means is provided for green prevention and control of the botrytis cinerea.

The invention discloses a method for preventing and treating diseases and pests of corncobs by mixed use of insecticidal diatomite, metarhizium anisopliae and trichoderma. According to the invention, the insecticidal diatomite, the metarhizium anisopliae CQMa421 dispersible oil suspending agent and / or the trichoderma asperellum GDFS1009 spore suspension are combined and sprayed on the ostrinia nubilalis larva body or ears or leaves infected by the ostrinia nubilalis and ear rot pathogen, the control effect on the ostrinia nubilalis reaches 60-80%, and the control effect on the ear rot caused by fusarium verticillium reaches 60-100%. The effect of preventing and controlling ostrinia nubilalis larvae and ear rot obviously exceeds that of an equivalent applicable single preparation, and synergistic green prevention and control of ear ostrinia nubilalis and ear rot are realized; the method is a novel green prevention and control technology for corn ear diseases and insect pests.

The invention discloses a cultivation method for promoting the growth of rhizoma bletillae and the accumulation of active ingredients, which comprises the following steps: carrying out foliage spraying on planted annual rhizoma bletillae seedlings by adopting a mesoporous silica loaded jasmonic acid methyl ester solution as a growth promoting agent, and meanwhile, applying trichoderma asperellum microcapsules to the roots of the annual rhizoma bletillae seedlings, the purposes of promoting growth of rhizoma bletillae and accumulation of active ingredients are achieved; by adopting the method, the growth of the rhizoma bletillae can be remarkably promoted, the content of active ingredients such as total polysaccharides, total phenols and total flavonoids in the rhizoma bletillae is effectively increased, meanwhile, the content of antioxidant ingredients is greatly increased, the yield and quality are both improved, and the method has important application value in rhizoma bletillae quality improvement cultivation.

The invention discloses a biological control method for cotton fusarium wilt. The invention provides a combined bacterium or a complex microbial inoculant containing the combined bacterium. The combined bacterium is prepared from AT actinoplanes (Actinoplanes) Q9316 (CGMCC (China General Microbiological Culture Collection Center) No.35443) and Trichoderma asperellum (Trichoderma asperellum) F2609 (CGMCC No.42159). The invention further provides a preparation method of the composite microbial inoculant. The fermentation liquid independently fermented by the trichoderma asperellum F2609 and the AT actinoplanes Q9316 provided by the invention has an inhibition effect on pathogenic bacteria of cotton fusarium wilt, and the bacteriostatic activity strength of the fermentation liquid jointly fermented by the trichoderma asperellum F2609 and the AT actinoplanes Q9316 exceeds that of the fermentation liquid fermented by single bacteria. The invention has important significance for biological prevention and control of cotton fusarium wilt.

The invention provides trichoderma asperellum HN-097 with high antagonism to hylocereus undulatus and application of the trichoderma asperellum HN-097. Wherein the plate confrontation result of the trichoderma asperellum HN-097 on the dragon fruit canker is 73.90%. The inhibition effect of the trichoderma asperellum HN-097 on the volatile substances of the dragon fruit canker is 8.13%. The inhibition effect of the trichoderma asperellum HN-097 on the non-volatile substances of the pitaya is 8.20%. The strain is identified as trichoderma asperellum through morphology and molecular biology. The preparation of the trichoderma oil suspending agent comprises 84.55% of soybean oil serving as an optimal oil carrier, 0.1% of boric acid serving as an optimal spore germination accelerant, 0.3% of ascorbic acid serving as an optimal ultraviolet protective agent, 0.05% of xanthan gum serving as an optimal adhesive and 5% of lignin calcium sulphonate serving as an optimal dispersing agent. When the trichoderma asperellum HN-097 oil suspending agent is used for preventing and treating the pitaya ulcer disease, the control effect of the trichoderma asperellum HN-097 oil suspending agent on pitaya fruits is 82%, and the control effect of the trichoderma asperellum HN-097 oil suspending agent on stalks is 51.18%. The trichoderma asperellum HN-097 can obviously reduce the incidence rate and the disease index of the dragon fruit canker, is green and environment-friendly, effectively reduces the use of chemical agents, and promotes the sustainable development of the dragon fruit industry.

The invention belongs to the technical field of microorganisms and particularly relates to a compound microbial agent as well as a preparation method and application thereof. The invention provides a compound microbial agent, the microbial agent comprises a paenibacillus polymyxa w-115 fermentation liquid and a trichoderma asperellum W1 spore liquid, the preservation number of the paenibacillus polymyxa w-115 is CGMCC (China General Microbiological Culture Collection Center) NO.30027, and the preservation number of the trichoderma asperellum W1 is CGMCC NO.40312. The invention further provides a preparation method of the compound microbial agent. The embodiment of the invention shows that the compound microbial agent can effectively promote vegetative growth of plants, improve fruit quality and improve plant root systems and soil microflora.