47 results about "Trichoderma asperellum" patented technology

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention relates to the technical field of agricultural microorganisms, in particular to trichoderma asperellum and application thereof.The trichoderma asperellum comprises at least one of the following trichoderma asperellum TA727, the trichoderma asperellum TA727 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO. 63859. The trichoderma asperellum has the advantages that the trichoderma asperellum can be used for preparing the trichoderma asperellum; the trichoderma asperellum is high in sporulation quantity and strong in acid resistance, and has a relatively good pathogenic bacterium inhibition effect under an acidic condition, and volatile substances of the trichoderma asperellum also have an antibacterial effect. The trichoderma asperellum can effectively inhibit pathogenic bacteria in an acid soil environment and soil-borne diseases caused by the pathogenic bacteria, meanwhile, industrial production can be facilitated, and the trichoderma asperellum has wide application prospects.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The application discloses application of Trichoderma asperellum strain YN4 and fungicides in prevention and treatment of plant fungal diseases. The application research shows that the Trichoderma asperellum strain YN4 has a good inhibitory effect on pepper anthracnose, rubber anthracnose, mango anthracnose, soybean anthracnose and the like. Further, compared with the single use of fungicides or the Trichoderma asperellum strain, the inhibitory effect of the combination of the two is better. Under certain conditions, the Trichoderma asperellum strain is combined with 10% difenconazole water dispersible granules, 70% thiophanate-methyl wettable powder or 450g / L prochloraz emulsion in water, and has a synergistic effect. Not only can the use amount of fungicides be greatly reduced, but also the inhibitory effect on plant pathogenic fungi can be further improved, the incidence can be reduced, the inhibition on pathogenic bacteria can be enhanced, the use and residue of chemical pesticides can be reduced, and technical support is provided for the selection of fungicides for pepper anthracnose and the like and the combination of bacteria and drugs.

The invention relates to the field of environmental pollution treatment and microorganisms, and discloses a preparation method of a trichoderma asperellum straw fermentation extracting solution and application of the trichoderma asperellum straw fermentation extracting solution in oxytetracycline pollution remediation or crop growth promotion. The method comprises the following steps: 1) performing solid-state fermentation on a trichoderma asperellum T1 seed solution to obtain a fermentation product; and 2) drying the fermentation product, adding the dried fermentation product into the leaching solution for leaching, and filtering to obtain the trichoderma asperellum straw fermentation extracting solution rich in trichoderma asperellum spores, enzyme and reducing sugar. The invention finds that the trichoderma asperellum T-1 has new application of repairing oxytetracycline pollution and promoting crop growth, can efficiently degrade oxytetracycline at normal temperature especially in the aspect of repairing oxytetracycline pollution, and has practical application value. Furthermore, compared with a non-extracted trichoderma asperellum leavening and pure trichoderma spores, the trichoderma asperellum straw fermentation extracting solution obtained by a specific process has a better oxytetracycline pollution repairing effect and a better crop growth promoting effect respectively in comparison with the non-extracted trichoderma asperellum leavening and the pure trichoderma spores.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

The invention relates to a composition with sleep aiding and skin repairing effects and a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a composition with sleep aiding and skin repairing effects. The composition is prepared from the following components in parts by weight: 72 to 114 parts of compound plant essential oil, 3 to 5 parts of ceramide NP and 1 to 3 parts of sodium hyaluronate, the compound plant essential oil is prepared from lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil and rhodiola rosea essential oil; the rhodiola rosea essential oil is obtained by fermenting a mixed strain composed of kluyveromyces lactis and trichoderma asperellum and then extracting essential oil. The research shows that the mixed strain fermentation can synergistically improve the extraction rate of the rhodiola rosea essential oil. In addition, the compound plant essential oil provided by the invention can also synergistically improve the anti-inflammatory, moisturizing and anti-allergy repair capabilities of the composition and has the effect of improving sleep disorders, so that the composition has better sleep aiding and skin repairing effects at the same time.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

The invention relates to a trichoderma asperellum sourced Cerata-plaatanin (CP) family protein CP3 and application thereof, and belongs to the technical field of biological prevention and control of plant diseases. The CP3 protein is derived from Trichoderma asperellum, belongs to a Cerato-platanin family and has a conservative CP structural domain, the amino acid sequence registration number of the CP3 protein is XP024756165.1, and the registration number of a coding gene of the CP3 protein is 36612810 (Gene ID). The protein is a secreting type protein, has signal peptide and can be positioned in tomato cell nucleuses. The invention proves that the disease spot area of tomatoes infected with Botrytis cinerea can be reduced by 71% after the CP3 protein is exogenously applied; after tomatoes are treated by the trichoderma asperellum CP3 overexpression strain, the area of gray mold scabs is remarkably reduced, and the CP3 gene knockout strain has no disease-resistant effect. The CP3 protein and the trichoderma asperellum strain expressing the CP3 protein can be used for preparing biological agents for enhancing the botrytis cinerea resistance, application modes comprise rhizosphere application, root system inoculation and the like, adverse effects on tomato growth are avoided, and a new effective means is provided for green prevention and control of the botrytis cinerea.

The invention discloses a method for preventing and treating diseases and pests of corncobs by mixed use of insecticidal diatomite, metarhizium anisopliae and trichoderma. According to the invention, the insecticidal diatomite, the metarhizium anisopliae CQMa421 dispersible oil suspending agent and / or the trichoderma asperellum GDFS1009 spore suspension are combined and sprayed on the ostrinia nubilalis larva body or ears or leaves infected by the ostrinia nubilalis and ear rot pathogen, the control effect on the ostrinia nubilalis reaches 60-80%, and the control effect on the ear rot caused by fusarium verticillium reaches 60-100%. The effect of preventing and controlling ostrinia nubilalis larvae and ear rot obviously exceeds that of an equivalent applicable single preparation, and synergistic green prevention and control of ear ostrinia nubilalis and ear rot are realized; the method is a novel green prevention and control technology for corn ear diseases and insect pests.

The invention discloses a biological control method for cotton fusarium wilt. The invention provides a combined bacterium or a complex microbial inoculant containing the combined bacterium. The combined bacterium is prepared from AT actinoplanes (Actinoplanes) Q9316 (CGMCC (China General Microbiological Culture Collection Center) No.35443) and Trichoderma asperellum (Trichoderma asperellum) F2609 (CGMCC No.42159). The invention further provides a preparation method of the composite microbial inoculant. The fermentation liquid independently fermented by the trichoderma asperellum F2609 and the AT actinoplanes Q9316 provided by the invention has an inhibition effect on pathogenic bacteria of cotton fusarium wilt, and the bacteriostatic activity strength of the fermentation liquid jointly fermented by the trichoderma asperellum F2609 and the AT actinoplanes Q9316 exceeds that of the fermentation liquid fermented by single bacteria. The invention has important significance for biological prevention and control of cotton fusarium wilt.

A Trichoderma asperellum HM-8 of the invention has the dual ability to antagonize Erwinia and Fusarium solani f. sp. batatas. The microbial agent prepared from the Trichoderma asperellum HM-8 strain can efficiently control sweet potato root rot and ginger stem basal rot. The spore count of Trichoderma asperellum HM-8 is not less than 108 CFU / g.

The application discloses a method for controlling ear diseases and pests of corn by using insecticidal diatomaceous earth, green muscadine and wood fungus in combination; the insecticidal diatomaceous earth, the dispersible oil suspension agent of the green muscadine beetle CQMa421 and / or the spore suspension of the echinoporus GDFS1009 are combined to be sprayed on the body of the corn borer larvae or the ears or leaves infected by the corn borer and the ear rot fungus, the control effect on the corn borer reaches 60-80%, and the control effect on the ear rot caused by the pseudorhizomycete fusarium reaches 60-100%. It is found that the control effects on the corn borer larvae and the ear rot are obviously higher than those of the same amount of single preparation, the synergistic green prevention and control of the ear corn borer and the ear rot is realized, and it is a new green prevention and control technology for the ear diseases and pests of corn.

The present application provides a strain of Trichoderma asperellum TAZ19, a biocontrol agent and application thereof, wherein the Trichoderma asperellum TAZ19 is preserved in China Center for Type Culture Collection on October 25, 2024, and the preservation number is CCTCC M 20242341. The active ingredient of the biocontrol agent comprises the above-mentioned Trichoderma asperellum TAZ19 strain, and the Trichoderma asperellum TAZ19 strain is one or more of conidium, mycelium, fermentation filtrate or active extract thereof. The Trichoderma asperellum TAZ19 has excellent antagonistic effect on multiple plant disease pathogens such as Diaporthe phaseolorum, multiple Fusarium oxysporum, Valsa mali, and Botryosphaeria dothidea, has broad-spectrum antibacterial property, fast growth, high antibacterial activity, strong parasitism, and good salt and alkali tolerance, and has a growth-promoting effect on plants. The Trichoderma asperellum TAZ19 can be widely applied to the prevention and treatment of multiple plant diseases such as pine branch and stem rot, wheat stem base rot, tomato wilt, and has a wide application prospect.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for saline-alkali soil and a biological bacterial fertilizer of the micro-ecological restoration liquid composite microbial agent. The micro-ecological restoration liquid complex microbial inoculant suitable for the saline-alkali soil is prepared from restoration salt-tolerant bacteria, namely bacillus subtilis, nocardia halospora, nitrogen-fixing bacteria, namely azotobacter chroococcum, phosphate solubilizing bacteria, namely pseudomonas fluorescens, potassium solubilizing bacteria, namely bacillus mucilaginosus, growth-promoting bacteria, namely azospirillum brasilense, biocontrol bacteria, namely bacillus megatherium and synergistic bacteria, wherein the restoration salt-tolerant bacteria are bacillus subtilis, nocardia halospora, the nitrogen-fixing bacteria are azotobacter chroococcum, the phosphate solubilizing bacteria are pseudomonas fluorescens, the potassium solubilizing bacteria are bacillus mucilaginosus, the growth-promoting bacteria are azospirillum brasilense, the biocontrol bacteria are bacillus megatherium, and the synergistic bacteria are prepared from bacillus subtilis The biological bacterial fertilizer for the saline-alkali soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the saline-alkali soil is greatly improved, the saline-alkali soil is effectively improved, and the crop yield is remarkably increased.

The application discloses a kind of fluorine-resistant trichoderma asperellum and the fungicide prepared by it, belong to microbial strain and application technical field.The strain is named Trichoderma asperellum M700, has been preserved in China typical culture preservation center (CCTC), and its preservation number is CCTCC NO: M 2024579, and the preservation date is March 27, 2024.The beneficial effect: the trichoderma asperellum strain of the application Trichoderma asperellum. Colony growth early stage presents white mycelium, later generation green spore, causes the body to present yellow green, green, aerobic, can survive at ph 3.0-10.0, temperature is 25-28 DEG C.The strain has very good resistance, and can grow when the fluorine ion concentration is below 800 mg / L.Can very good dispel fluorine element in water body.

The application discloses a method for synthesizing lactic acid from lignocellulose by using a fungus-bacterium mixed culture system with functional complementation, and comprises the following steps: (1) inoculating activated Trichoderma asperellum into a fermentation medium containing microcrystalline cellulose to perform fermentation, and obtaining a fermentation liquor; and (2) inoculating activated Lactobacillus paracasei into the fermentation liquor obtained in the step (1) to perform anaerobic fermentation and produce lactic acid. When the Lactobacillus paracasei is inoculated when the Trichoderma asperellum is cultured for 40 hours, the highest lactic acid yield is achieved, and reaches 57.59 g / L. The method is currently used to obtain the highest lactic acid yield in the mixed culture fermentation by using microcrystalline cellulose as the only carbon source. Meanwhile, the method can also be applied to a fermentation process for directly synthesizing lactic acid from lignocellulose. When the fermentation medium with corn cobs as the substrate is used to perform fermentation, 9.80 g / L of lactic acid can be produced, the substrate cost for industrial production of lactic acid is greatly reduced, and the method has important application value.

The invention relates to a method for producing an organic water-soluble fertilizer by utilizing marigold fermentation press filtrate. Belongs to the fertilizer field. The compound fertilizer comprises a marigold water-soluble fertilizer and rosemary fermentation liquor, the marigold water-soluble fertilizer is obtained by performing pH adjustment on marigold fermentation press filtrate, performing solid-liquid separation, taking liquid, concentrating, cooling, settling and taking liquid. The rosemary fermentation liquor is obtained by fermenting rosemary waste residues through trichoderma asperellum, carrying out solid-liquid separation and taking liquid. According to the invention, two agricultural wastes are converted into high-value functional fertilizers, and integration of resource circulation and pesticide-fertilizer is realized. Through biological fermentation, the marigold water-soluble fertilizer provides growth-promoting nutrition and improves capsaicin synthesis, and trichoderma asperellum viable bacteria are introduced into rosemary fermentation liquor, so that pepper phytophthora blight is prevented and treated specially. The water-soluble fertilizer provided by the invention is especially suitable for pepper planting, and is also a universal water-soluble fertilizer.

The application discloses a kind of fungus reinforced erythromycin fermentation microbial residue aerobic compost efficient detoxification and nutrient recovery method, belong to solid waste disposal and resource recycling field.The method includes that the erythromycin fermentation microbial residue is mixed after being treated with high-temperature sterilization with corn straw powder, inoculates and carries out aerobic compost fermentation with Trichoderma asperellum.The antibiotic degrading inoculum with Trichoderma asperellum as core is inoculated to reinforce the aerobic co-composting of erythromycin fermentation microbial residue and crop straw, produces nutrient content high and non-toxic harmless organic fertilizer, to provide an efficient, economical, environmentally friendly solution to solve the problem of resource utilization of erythromycin fermentation microbial residue.

This invention discloses a bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers. By weight, the bio-fertilizer comprises the following raw materials: 30-40 parts of well-rotted livestock and poultry manure, 20-30 parts of straw or rice husks, 1-2 parts of borax, 2-3 parts of urea, 2-4 parts of ammonium sulfate, 2-3 parts of zinc sulfate, 2-3 parts of magnesium sulfate, 1-2 parts of humic acid, and 1-2 parts of a microbial compound agent. The microbial compound agent comprises 4-6 parts of Bacillus megaterium, 2-3 parts of Bacillus polymyxa, 2-4 parts of Trichoderma echinococcus, 2-3 parts of Trichoderma viride, 3-5 parts of Pseudomonas fluorescens, 4-5 parts of Azotobacter chrysogenum, and 2-4 parts of Candida albicans. Using this bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers can significantly reduce the impact of continuous cropping obstacles, not only significantly reducing the incidence of cucumber wilt and root-knot nematodes and significantly promoting cucumber seedling growth, but also significantly increasing cucumber yield.

This invention discloses a method for improving methanogenesis from lignocellulose based on a mixed-culture fermentation strategy, belonging to the field of anaerobic fermentation technology. The method includes the following steps: (1) inoculating activated *Trichoderma echinosporum* into a culture medium containing lignocellulose and loofah sponge as a biological carrier material for fermentation to obtain a fermentation broth; (2) activating activated sludge using a revitalizing solution; (3) inoculating the activated sludge from step (2) into the fermentation broth of *Trichoderma echinosporum* from step (1) for anaerobic fermentation to produce methanogens. This method achieves a methane yield of 262 mL / g VS, which is more than 30% higher than that of traditional anaerobic fermentation for methanogenesis, and is of great significance for improving the bioconversion of lignocellulose resources.

The invention belongs to the technical field of microorganisms, and discloses trichoderma asperellum and application thereof in prevention and treatment of wheat scab. The invention provides trichoderma asperellum which is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66459. The trichoderma asperellum provided by the invention has a relatively good prevention and treatment effect on wheat scab and has a growth promoting effect on wheat.

The application of a strain of Trichoderma asperellum SFC-3 in degrading carfentrazone-ethyl. The Trichoderma asperellum SFC-3 is capable of growing by using carfentrazone-ethyl as the sole carbon and nitrogen source and degrading carfentrazone-ethyl. The present application provides the fungal strain Trichoderma asperellum SFC-3 with high-efficiency degradation effect on carfentrazone-ethyl for the first time. The strain is capable of growing by using carfentrazone-ethyl as the sole carbon and nitrogen source, has a wide degrading environmental temperature (the degrading capacity is the strongest at 25 DEG C, and the strain can also grow at 40 DEG C), and a wide pH value (the degrading effect is the best at a pH value of 8, and the strain also has better degrading capacity within the pH value range of 6-10). Meanwhile, the strain has simple culture conditions, is easy to be industrialized, and has significant social and economic benefits.

The invention belongs to the technical field of agricultural biology, and particularly relates to trichoderma asperellum 1271 for specifically preventing and treating root-knot nematode and application of the trichoderma asperellum 1271. The invention provides the trichoderma asperellum 1271 with the preservation number of CGMCC No.42153, hyphae of the trichoderma asperellum 1271 are white filamentous and grow rapidly, conidia close to the center are dark green, conidiophore is dispersed in the whole bacterial colony or gathered into concentric rings, the conidia are spherical, and the trichoderma asperellum 1271 can be used for preventing and treating root-knot nematode, especially cucumber phaseolus calcaratus root-knot nematode. On the basis, the invention further provides a bacterium-pesticide combination for preventing and treating the root-knot nematode, after the bacterium-pesticide combination is applied to cucumbers, an excellent prevention and treatment effect is achieved for the auricularia auricula-bean root-knot nematode, and the prevention effect of the bacterium-pesticide combination is obviously superior to that of a chemical agent with the same concentration and a single biocontrol bacterium; meanwhile, the dosage of chemical agents is effectively reduced, the generation of the drug resistance of pathogens to the chemical agents is retarded, and the fungicide combination is environment-friendly, green and safe.

The invention provides trichoderma asperellum SWU Y1-45 and application thereof, and relates to the technical field of biological control. Comprising trichoderma asperellum SWU Y1-45, the classification name of the trichoderma asperellum is Trichoderma asperellum, the trichoderma asperellum SWU is preserved in China General Microbiological Culture Collection Center on May 22, 2025, the preservation number of the trichoderma asperellum SWU is CGMCC No.41950, and the preservation address of the trichoderma asperellum SWU is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The compound has a good inhibition effect on plant pathogenic fungi such as rhizoctonia binuclear, fusarium, phaseolus vulgaris, colletotrichum gloeosporioides and trichosporon actinosporium, and can realize efficient prevention and treatment on sweet osmanthus root rot, ginkgo basal rot, loquat anthracnose and loquat stem rot. The invention also provides a derivative of trichoderma asperellum SWU Y1-45, which is applied to practice, can also effectively realize prevention and treatment of sweet osmanthus root rot, ginkgo basal rot, loquat anthracnose and loquat stem rot, and has a wide application prospect in agricultural green production.

The invention discloses a method for improving the quality of fresh corn. The method mainly comprises the following steps: treating corn seeds by using a fresh corn biological seed coating agent, wherein the biological seed coating agent comprises trichoderma asperellum fermentation liquor, bacillus velezensis fermentation liquor, chitosan, thyme oil, locust bean gum and diatomite; the total volume of the trichoderma asperellum fermentation liquor and the bacillus velezensis fermentation liquor is 100 mL, and the proportions of the rest components in the total fermentation liquor are as follows: 0.1-0.3 g / ml of chitosan, 0.5-2 g / ml of thyme oil, 3-5 g / ml of locust bean gum and 5-10 g / ml of diatomite; and the dosage of the biological seed coating agent is 2600-3000 particles according to the ratio of the biological seed coating agent to the seed of 10g. The fresh corn seed coating agent provided by the invention can reduce the incidence rate of stem rot of fresh corn on one hand, and can increase carotenoid, lutein content, amylose content, cellulose content, titratable acid and the like in the fresh corn on the other hand.

The present application relates to a kind of suitable for poplar growth regulating composition and biological preparation, including functional bacteria agent and chemical trigger complex;Functional bacteria agent includes Bacillus amyloliquefaciens and poplar canker antagonistic Trichoderma asperellum;Chemical trigger complex includes the directional metabolic promotion group for promoting the directional synthesis of functional bacteria agent plant hormone, the metal cofactor signal group for strengthening the effect of directional metabolism, and the fermentation microenvironment regulation group for providing initial microenvironment support for the fermentation of functional bacteria agent under poplar;Directional metabolic promotion group includes L-tryptophan and adenine;Metal cofactor signal group includes manganese ion signal and magnesium ion signal;Fermentation microenvironment regulation group includes humic acid salt, mineral phosphorus source, clay mineral carrier and sugar protective agent.The present application can solve the problems existing in the prior art, such as the difficulty of regulating composition to realize the directional growth of poplar trunk, the need for multiple applications, the high risk of ecological residue, and the difficulty of cost to adapt to the large-area cultivation of poplar.