59 results about "Rhizoctonia" patented technology

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

According to the nano pesticide and the preparation method and application thereof, pH response type nano particles ZIF-90 serve as a carrier, azoxystrobin is loaded to serve as a medicine, and the nano pesticide is used for preventing and treating rice sheath blight disease caused by rhizoctonia solani. When the rhizoctonia solani infects rice, oxalic acid can be generated to reduce the pH around an infected area, so that the ZIF-90 carrier skeleton and the hyaluronic acid coating are decomposed to release azoxystrobin, and the rhizoctonia solani is inhibited in a targeted manner. It is further found that ZIF-90 and azoxystrobin have synergistic bacteriostatic activity, the EC50 value of azoxystrobin to rhizoctonia solani can be greatly reduced, after ZIF-90 carrier encapsulation and hyaluronic acid end capping, the photolysis stability of azoxystrobin can be improved, the adhesive force of azoxystrobin on rice leaves can be enhanced, and the ZIF-90 and azoxystrobin compound has important application in agricultural disease control.

The invention belongs to the technical field of microorganisms, and particularly discloses trichoderma harzianum NBU-1 and application thereof. The Trichoderma harzianum NBU-1 is preserved in the Guangdong Microbial Culture Collection Center on March 26, 2025, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCCNO: 66053. The invention discloses trichoderma harzianum NBU-1 and application thereof, the trichoderma harzianum NBU-1 is applied to prevention and treatment of wheat sharp eyespot, and the activity of rhizoctonia cerealis R0301 and the growth of scab are effectively inhibited.

The application provides a streptomyces LZZY-S3 and application thereof, and belongs to the field of microorganisms.The streptomyces LZZY-S3 provided by the application enriches strains for biological control, and realizes the dual functions of plant resistance to pathogenic bacteria and plant promotion.The streptomyces LZZY-S3 has the function of inhibiting fungi in a wide range, and can be applied to plant resistance to pathogenic bacteria, and the pathogenic bacteria include Exobasidium vexans, Botryosphaeria spp., Gibberella, Fusarium oxysporum, Pythium, Fusarium graminearum, Didymella, Fusarium pseudograminearum, Trichothecium roseum, Coniella diplodiella, Colletotrichum graminicola or Rhizoctonia cerealis, wherein the inhibition rate of Exobasidium vexans reaches 91.2%.The streptomyces LZZY-S3 can also be applied to plant promotion, preparation of nitrogen-fixing bacterial agents and preparation of ACC deaminase bacterial agents and the like.

The invention discloses a soybean phomopsis longicolla resistance gene and application thereof, and belongs to the field of genetic engineering, the gene is Psd18.1, and the resistance of soybeans to the soybean phomopsis longicolla can be improved by knocking out or silencing the soybean Psd18.1 gene. It is proved that the Psd18.1 negatively regulates and controls the resistance of the soybeans to the PSD. The invention provides a new choice for preventing and treating the soybean phomopsis seed rot and cultivating disease-resistant varieties.

The present application belongs to the technical field of orchid disease prevention and treatment, and particularly relates to a fungicidal composition for preventing and treating orchid root rot. The fungicidal composition for preventing and treating orchid root rot comprises active ingredients of nerol and compound a, wherein the compound a is at least one selected from prochloraz, difenoconazole and carbendazim. When nerol is compounded with prochloraz, difenoconazole and carbendazim, the synergistic effect is shown by the co-toxicity coefficient (CTC) value of the compound combination to the pathogenic fungus of Rhizoctonia solani being greater than 120, which indicates that the compound combination can improve the prevention and treatment effect on orchid root rot. In addition, the fungicidal composition contains two effective ingredients, which can delay the generation and development of pathogenic fungi resistance and overcome the problems existing in long-term use of single component fungicides. In summary, the fungicidal composition can be used for developing fungicides for preventing and treating orchid root rot.

The invention discloses a virens and white beard fungus strain and application thereof, and belongs to the technical field of biology. The preservation number of the white beard virens SJ-50F is CCTCC (China Center For Type Culture Collection) NO: M 20251911, and the white beard virens SJ-50F has a remarkable inhibition effect on a rice sheath blight disease pathogenic bacterium rhizoctonia solani YM-Q-7. According to the invention, an in-vitro confrontation culture and potting verification method is adopted, which proves that the virens SJ-50F can inhibit the growth of germs, a plate confrontation experiment shows that the hypha growth inhibition rate of YM-Q-7 reaches 82.36%, and the hypha growth inhibition rate of fermentation supernate of the virens SJ-50F to YM-Q-7 reaches 90.79%.

The present application provides a kind of compound biocontrol agent and its preparation method and application technology, and it is composed of 1000ml of fermentation mixed liquor of multiple strains of biocontrol bacteria mixed in equal volume ratio and 0.5%-1.5% stabilizer with complementary mechanism and target.The fermentation concentrated solution of multiple strains of bacteria is prepared in fermentation medium to obtain the present application, which is used for preventing and controlling fusarium, pythium, phytophthora, rhizoctonia solani, ralstonia solanacearum, erwinia carotovora, streptomyces scabiei, xanthomonas campestris and other plant pathogenic bacteria and nematodes, which can inhabit in soil for a long time and spread through soil, and one or several of them can cause plant soil-borne diseases.The preparation process of the present application is not affected by too many chemical and physical factors, the activity of live bacteria and metabolites is high, the average control effect is 78%-85%, the application effect is good;The production process is simple, energy and auxiliary materials are saved, and the production cost is low.

The invention discloses streptomyces alboflavus (with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35588) for preventing and treating muskmelon fusarium wilt, and belongs to the field of biological prevention and treatment. The strain is high in sporulation quantity and strong in colonization capacity, pathogenic bacteria are inhibited and growth is promoted through antagonism, secondary metabolites, induction system resistance and other mechanisms, the potting control effect reaches 86.3%, and the effect lasts for 21 days. The streptomyces albidoflavus not only can prevent and treat fusarium wilt of muskmelons, but also has a broad-spectrum inhibition effect on fusarium wilt of cucumbers, watermelons and tomatoes and soil-borne diseases such as pepper phytophthora blight and rhizoctonia solani, and can improve rhizosphere microecology and promote reproduction of effective microbial communities. The biocontrol microbial agent is a pure biological agent, is pollution-free and nuisanceless, and can remarkably reduce the use of chemical pesticides, relieve continuous cropping obstacles and promote green development of the watermelon and melon industry.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

This invention belongs to the field of microbial control technology, specifically relating to a biocontrol bacterium for soybean root rot, a microbial agent, its preparation method, and its application, which can improve soybean yield and oil content. The biocontrol bacterium is *Alcaligenes faecalis* (Factophytes). Alcaligenes faecalis A new strain, named GY16, was deposited at the China Center for Type Culture Collection (CCTCC) on November 12, 2025, with accession number CCTCC M 20252536. This alkali-producing bacterium, GY16, exhibits a specific affinity for soybean, enabling it to colonize soybean roots and effectively control soybean root rot. GY16 also promotes soybean plant growth, increases soybean yield, and improves seed oil content. Inoculants prepared using GY16 can replace pesticides, offering a green, environmentally friendly, and pollution-free alternative that positively contributes to building a sustainable farmland ecosystem.

The invention discloses a rhizoctonia solani fusion group AG-3 basidiospore separation method, and belongs to the technical field of biology, the rhizoctonia solani fusion group AG-3 basidiospore separation method comprises the following steps: S1, preparing a rhizoctonia solani fusion group AG-3 spore suspension; and S2, collecting tender tobacco leaves, disinfecting, inoculating the spore suspension obtained in the step S1 on the disinfected tobacco leaves, culturing, regularly sampling and observing, and separating and purifying the scabs again after the scabs are observed, so as to obtain a bacterial strain which is a newly formed basidiospore bacterial strain after spore germination. The invention discloses a method for inducing rhizoctonia solani fusion group AG-3 basidiospore by using tobacco leaves, the whole separation method is simple to operate, and analysis and research on pathogenicity, pathogenic mechanism, polygene systematics and genetic diversity of a rhizoctonia solani fusion group AG-3 single spore strain are facilitated.

The invention relates to the technical field of bacteriostatic preparations, in particular to mugwort essential oil as well as a preparation method and application thereof. Mixing mugwort with a sodium chloride solution, and soaking to obtain a soaked material; the material-liquid ratio of the soaked material is 4-12 mL / g; the mass percentage content of sodium chloride in the sodium chloride solution is 5-20%; the soaking time is 1-4 hours; distilling the soaked material, standing and layering distillate, and collecting upper-layer essential oil to obtain artemisia argyi essential oil; the distillation time is 1 to 5 hours. The mugwort essential oil provided by the invention has a relatively good inhibition effect on the growth of common plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum and fusarium oxysporum, so that the mugwort essential oil is used as a biological bactericide in future agricultural production, and the effect of preventing and treating diseases can be achieved to a certain extent.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas aeruginosa and application thereof. The invention discloses Pseudomonas aeruginosa R5-2, and the number of the Pseudomonas aeruginosa R5-2 is CGMCC (China General Microbiological Culture Collection Center) No.34796. The invention further discloses a preparation method of the Pseudomonas aeruginosa R5-2. The strain can secrete various active substances such as siderophores and chitinase, and can effectively inhibit growth of various pathogenic bacteria such as fusarium oxysporum, verticillium dahliae, rhizoctonia solani and fusarium graminearum. Experiments show that a fungicide containing the strain has a remarkable prevention and treatment effect on lycium barbarum root rot and tomato fusarium wilt caused by fusarium oxysporum. The invention provides a novel and environment-friendly microbial resource for preventing and treating soil-borne fungal diseases of crops such as medlar, tomato and the like, and has important application value.

The invention relates to the technical field of biology, in particular to a defensive protein derived from konjak and having broad-spectrum antibacterial activity, a coding gene and application of the defensive protein and the coding gene. According to the invention, the defensive protein is obtained from konjak for the first time, and the defensive protein has an obvious inhibition effect on multiple fungi (rhizoctonia solani, fusarium oxysporum, fusarium aromatic, fusarium solani and phytophthora nicotianae) and bacteria (bacillus cereus and ralstonia solanacearum). The defensive protein has wide application prospects in the fields of food, hygienic products, cosmetics, biopesticides, biological feed additives or natural food preservatives and the like.

The invention provides application of an OsNAC15 gene in improving resistance of rice to rhizoctonia solani, and belongs to the technical field of biology. The invention provides application of a reagent for inhibiting the expression of the OsNAC15 gene in improving the resistance of rice to rhizoctonia solani or improving the resistance of rice to sheath blight, provides negative regulation of the resistance of the rice to rhizoctonia solani by the OsNAC15 for the first time, and supplements the function of the OsNAC15 in interaction of the rice and rhizoctonia solani and a disease-resistant pathway for regulating a PR gene by an NAC transcription factor; and a new thought is provided for research on a rice-rhizoctonia solani interaction mechanism.

The application discloses a Rosa chinensis disease prevention and growth promotion bacterium LX6 strain and application thereof, and belongs to the field of microorganism strains and application thereof. The Rosa chinensis disease prevention and growth promotion bacterium LX6 is preserved in the China General Microbiological Culture Collection Center on May 16, 2024, and has a preservation number of CGMCC No. 30657 and a classification name of Pseudomonas fluorescens. The bacterium strain has the ability to resist high-concentration salt ions and various heavy metal ions. The bacterium strain can inhibit the growth of a Rosa chinensis black spot pathogen, i.e., Discosia rosea, and can also antagonize the growth of plant pathogenic fungi, i.e., Colletotrichum gloeosporioides, Rhizoctonia solani, Fusarium oxysporum and Fusarium equiseti, so as to prevent and treat the Rosa chinensis black spot and powdery mildew, and promote the growth and flowering of the Rosa chinensis. The application provides a fine biocontrol strain for preventing and treating the diseases of the Rosa chinensis and promoting the growth of the Rosa chinensis, and has important significance for green prevention and control technology of the diseases of the Rosa chinensis and sustainable development of the Rosa chinensis industry.

The application provides a method for screening soybean Cercospora sojina blight response genes based on transcriptome analysis, which comprises the following steps: selecting pods of soybean Cercospora sojina blight infected plants and healthy plants planted in the field, performing transcriptome sequencing, screening differential expression genes of soybean responding to Cercospora sojina blight, analyzing biological pathways of the differential expression genes in the Cercospora sojina blight infection process, performing protein interaction analysis, screening interaction core genes, comparing sequences, performing transcript from scratch assembly, analyzing differential variable splicing events and exon usage differences through integration analysis, analyzing post-transcriptional regulation network dynamic characteristics of key candidate genes of soybean responding to Cercospora sojina blight, and screening Cercospora sojina blight response genes of soybean. Four Cercospora sojina blight response genes of soybean are obtained, and expression characteristics in different soybean tissues are analyzed, which provides a theoretical basis for soybean Cercospora sojina blight resistance breeding.

The invention discloses preparation and application of a microbial agent for preventing and treating damping-off, relates to the technical field of damping-off prevention and treatment, and solves the technical problems of pesticide residues and environmental pollution caused by adopting chemical pesticides to prevent and control damping-off in the prior art. The streptomyces fungicide for preventing and treating damping-off is streptomyces fermentation liquor prepared by taking streptomyces pseudogriseogriseus YWF-21-2116 as a strain and carrying out seed activation and at least three-stage culture on the streptomyces pseudogriseogriseus YWF-21-2116. The streptomyces inoculant for preventing and treating damping-off is applied to agricultural production, the use amount of chemical pesticides can be effectively reduced, and the development of green agriculture is promoted.

The invention relates to a novel acridinyl-containing tetrahydronaphthalene spiro isoxazoline compound, a preparation method thereof and application of the novel acridinyl-containing tetrahydronaphthalene spiro isoxazoline compound as an antifungal drug. The novel acridinyl-containing tetrahydronaphthalene spiroisoxazoline compound has an inhibition effect on parasite sphaerospora, botrytis cinerea, rhizoctonia solani, fusarium graminearum and candida albicans in the antifungal activity of the novel acridinyl-containing tetrahydronaphthalene spiroisoxazoline compound. An antifungal biological activity result shows that the compound shows remarkable antifungal activity, has comprehensive antifungal characteristics and is determined as a broad-spectrum antifungal candidate, the EC50 value of the compound to parasitic powdery spores is superior to that of a positive control drug chlorothalonil, and the compound shows about 12 times and 25 times of titer advantages compared with famoxadone and carbendazim respectively. The spiro structure and multiple active groups of the compound can significantly improve the antifungal activity of the compound, and in addition, the compound has protection and treatment effects on powdery parasitic spore infection in corn, which shows that the compound has agricultural application potential.

The present invention relates to a microbial strain having superior fungicidal and / or nematicidal activity, as well as its progeny and derivatives, compositions comprising the same, and methods of using the same, inter alia in agriculture. The strain, as well as its progeny and derivatives, is capable of increased fungicidal activity against at least one fungi selected from the group consisting of: Botrytis, Fusarium, and Rhizoctonia when compared to Bacillus velezensis strain GB03.

The invention relates to application of trichoderma harzianum to prevention and treatment of potato black scurf and scab. The trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.40017 can well inhibit rhizoctonia solani, so that the effect of preventing and treating potato black scurf is achieved; trichoderma harzianum with the preservation number of CGMCC No.40017 can also inhibit streptomycete, such as streptomyces scabies, streptomyces scabies and streptomyces griseus, so that the effect of preventing and treating potato scab is achieved, the strain or the microbial agent containing the strain is applied to the planting process of potatoes, potato black scurf and scab can be effectively prevented and treated, and the effect of preventing and treating potato scab is achieved. The method has important significance in agricultural production.

The application discloses a bacillus velezensis BZWQ55, a microbial agent and application thereof, and belongs to the field of microorganisms. The bacillus velezensis BZWQ55 is screened from rhizosphere soil of potato scab and has a preservation number of CGMCC No. 36805. Experimental results show that the bacillus velezensis BZWQ55 has the abilities of producing cellulase, protease, amylase and iron carrier, and has the abilities of phosphorus and potassium solubilization and nitrogen fixation, and can effectively promote plant growth; the bacillus velezensis BZWQ55 can also stably inhibit the growth of Fusarium solani, Fusarium oxysporum, Alternaria solani, Streptomyces scabrisporus, Rhizoctonia solani and Neocosmospora vasinfecta, and has a significant prevention effect on multiple soil-borne diseases such as potato scab, sweet potato root rot, potato scab and peanut fruit rot. The application provides a new strain resource for biological control of plant soil-borne diseases and promotion of plant growth.

The invention discloses an application of any compound of an Oxoapophine alkaloid derivative in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative shows certain inhibitory activity on two plant pathogenic bacteria such as rice bacterial blight and citrus canker pathogen, and five plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, magnaporthe grisea and wheat stem rot; the compound has a good inhibition effect on citrus canker pathogen and sclerotinia sclerotiorum. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The invention belongs to the technical field of bioengineering, and relates to xylanase derived from rhizoctonia thermophila and application of the xylanase. The amino acid sequence of the enzyme is as shown in SEQ ID NO. 2. The nucleotide sequence of the gene for coding the enzyme is as shown in SEQ ID NO. 1. The xylanase provided by the invention can specifically degrade xylan without damaging a fiber structure, maintains activity under an alkaline high-temperature condition, can reduce the use amount of ClO2 in paper pulp pretreatment, and shows a good bleaching assisting effect.

The invention discloses a composition containing pydiflumetofen and epoxiconazole as well as a preparation method and application of the composition, and belongs to the technical field of pesticides. The bactericidal composition disclosed by the invention takes the pydiflumetofen and the epoxiconazole as main active components, has a synergistic effect, has a wider bactericidal spectrum, particularly has an excellent synergistic prevention and treatment effect on diseases caused by fusarium and rhizoctonia, and can effectively delay the generation of drug resistance of pathogenic bacteria, improve the prevention and treatment effect and prolong the lasting period; besides, tetradecyl methyl dihydroxyethyl ammonium bromide is introduced into the modified bentonite, the bactericidal effect can be enhanced, organic silicon is introduced into the surface of the modified silicon dioxide, the modified silicon dioxide can also be adsorbed on the surfaces of bacteria, the seepage force to cell walls is enhanced, and the bactericidal effect is further improved. On the whole, the dispersion stability is excellent, particle aggregation and caking are prevented, the uniformity and stability of the product in the storage and application process are guaranteed, and the sterilization effect is more excellent.

The present invention belongs to the field of microorganisms, and specifically relates to a banana endophyte compound agent, a preparation method, and an application thereof. The present invention isolates a novel endophytic Bacillus Velez JDB15 and an endophytic Trichoderma harzianum JDL4 from banana plants. The Trichoderma harzianum JDL4 can inhibit Botrytis cinerea, Fusarium graminearum, Rhizoctonia solani, Colletotrichum syringae prunellae, Peach anthracnose, Peach brown rot, and Alternaria alternata, and has a good broad-spectrum antibacterial effect. At the same time, the filtrate of the fermentation broth of Trichoderma harzianum JDL4 is compounded with Bacillus Velez JDB15 to significantly enhance the antibacterial effect, and the control effect against banana wilt pathogen, rice blast pathogen, tomato wilt, and corn leaf spot pathogen is also significantly enhanced. The compound agent has a wide range of biocontrol activities and can be further developed in the future to provide a sustainable and green solution for the control of banana wilt and other plant diseases.

The invention provides trichoderma asperellum SWU Y1-45 and application thereof, and relates to the technical field of biological control. Comprising trichoderma asperellum SWU Y1-45, the classification name of the trichoderma asperellum is Trichoderma asperellum, the trichoderma asperellum SWU is preserved in China General Microbiological Culture Collection Center on May 22, 2025, the preservation number of the trichoderma asperellum SWU is CGMCC No.41950, and the preservation address of the trichoderma asperellum SWU is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The compound has a good inhibition effect on plant pathogenic fungi such as rhizoctonia binuclear, fusarium, phaseolus vulgaris, colletotrichum gloeosporioides and trichosporon actinosporium, and can realize efficient prevention and treatment on sweet osmanthus root rot, ginkgo basal rot, loquat anthracnose and loquat stem rot. The invention also provides a derivative of trichoderma asperellum SWU Y1-45, which is applied to practice, can also effectively realize prevention and treatment of sweet osmanthus root rot, ginkgo basal rot, loquat anthracnose and loquat stem rot, and has a wide application prospect in agricultural green production.

The present application provides a kind of plant pathogenic soil biological control agent and its preparation method and application technology, which is composed of multiple different genera of biocontrol strains and their metabolite concentrated solid 70-90% and auxiliary materials 10-30% with complementary mechanism and target.The fermentation concentrated liquid of multiple strains is mixed with carrier, and then spray dried to obtain the compound bacteria fermentation liquid concentrated solid;Then it is mixed with auxiliary materials to granulate to obtain the present application.The present application is used for the prevention and control of plant diseases caused by one or several of fusarium, pythium, pathogenic streptomyces, rhizoctonia solani, raoultella, erwinia carotovora and other plant pathogenic bacteria and nematodes that can survive in soil and its pathogenic soil.The present application effectively improves the microecological environment of pathogenic soil, induces plant disease resistance effect, constructs a good plant soil ecosystem and promotes plant growth and development.

This invention belongs to the field of agricultural microbiology technology, specifically relating to a microbacterium strain and its application in plant disease control. This invention discloses a microbacterium strain screened from wolfberry roots, named... Microbacterium sp. R5-1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 34797, has the 16S rDNA sequence shown in SEQ ID NO:1. It secretes siderophores, cellulase, and protease. Experimental results demonstrate that this strain exhibits inhibitory activity against the growth of *Fusarium oxysporum*, *Rhizoctonia solani*, and *Verticillium dahliae*, and shows significant control efficacy against soil-borne fungal diseases of plants caused by these fungi. This invention provides an important microbial resource for the green control of soil-borne fungal diseases of plants.