117 results about "Rhizoctonia" patented technology

The invention provides bacillus velezensis NJ157 and application thereof, and relates to the technical field of biological pesticides. The bacillus velezensis NJ157 can resist saline and alkaline, has a good inhibition effect on ralstonia solanacearum, rhizoctonia solanacearum, fusarium moniliforme and phytophthora capsici, and can effectively prevent and treat tomato bacterial wilt, root rot and fruit rot. The bacillus velezensis NJ157 disclosed by the invention also has relatively strong capability of producing protease, cellulase and IAA (Indoleacetic Acid), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.

The invention provides bacillus paralicheniformis NJ102 and application thereof, and belongs to the technical field of microorganisms. The bacillus paralicheniformis NJ102 disclosed by the invention has the capabilities of resisting low temperature and saline and alkaline, and can endure the low temperature of 10 DEG C, the salt stress with the salt content of 17% and the strong alkali stress with the pH value of 12.5. Meanwhile, the bactericidal composition has a relatively good inhibition effect on ralstonia solanacearum, rhizoctonia solanacearum and phytophthora capsici, and can effectively prevent and treat tomato bacterial wilt, root rot and fruit rot. The bacillus paralicheniformis disclosed by the invention also has relatively strong capability of producing protease, cellulase, amylase and IAA (Indoleacetic Acid), has a relatively good growth promoting effect on tomatoes, and has great application potential in the aspect of biopesticide.

The invention provides a novel strain M2108 of a salt-tolerant bacillus, the preservation number of the salt-tolerant bacillus is CGMCC (China General Microbiological Culture Collection Center) No.28283, and the novel strain M2108 of the salt-tolerant bacillus has relatively good salt tolerance and can grow and proliferate in a high-salt environment; the strain can be rapidly and stably colonized in a plant, and is rapid in propagation speed and strong in multiplication capacity in the plant. The new strain M2108 of the salt-tolerant bacillus provided by the invention has a treatment effect and a high control effect on various plant diseases: the control effect on diseases such as tomato root rot, damping-off and damping-off caused by fusarium oxysporum, pythium aphanidermatum and rhizoctonia solani is high, and the average control effect is 91.2% or above. When being used for preventing and treating plant diseases, the salt-tolerant bacillus M2108 is not easy to generate drug resistance, has good drug effect durability, is safe to human and livestock, and does not cause environmental pollution; the salt-tolerant bacillus M2108 microbial agent and the biocontrol agent provided by the invention are simple in preparation method, low in cost and simple and convenient to use. The salt-tolerant bacillus M2108 has a good application prospect in the prevention and control of plant diseases, and lays a foundation for the development of efficient and broad-spectrum biocontrol preparations.

The invention relates to bacillus velezensis and application thereof in prevention and treatment of wheat leaf blight and inhibition of plant pathogenic fungi, and belongs to the field of biological prevention and treatment. The bacillus velezensis HXYT7 is separated and screened from tobacco leaves growing in a tobacco plantation (the longitude is 113.73 and the latitude is 33.91) in Xiangchang County in elm city, Henan province, has a remarkable inhibition effect on mophila, fusarium moniliforme, fusarium graminearum, rhizoctonia cerealis, aspergillus flavus and aspergillus niger, can damage the hypha structure of the mophila, and can be used for preparing the bacillus velezensis HXYT7. The bacillus subtilis can inhibit germination of spores of the helminthosporium, has an obvious prevention and treatment effect on the wheat leaf blight caused by the helminthosporium, and provides a new microbial resource for prevention and treatment of the wheat leaf blight.

The invention belongs to the technical field of microorganisms, and discloses an actinomycete, which is streptomyces aureoflavus CYS4-5, is preserved in China General Microbiological Culture Collection Center (CGMCC), is preserved on October 17, 2023, and has the preservation number of CGMCC No.28506. The actinomycete has the advantages that the actinomycete can be used for preparing an actinomycete culture medium; the strain has the potential of preventing and treating salvia miltiorrhiza root rot, has obvious bacteriostatic activity on gaeumannomyces graminis, rhizoctonia cerealis, exserohilum turcicum, sclerotinia sclerotiorum, wheat root rot, fusarium graminearum and botryosphaeria dothidea, and plays an important role in biological prevention and treatment of diseases of crops such as wheat, corn, rape and grapes.

The content of IAA produced by the enterobacter ludwigii XL0404 can reach 18.94 mg / L, the content of ACC deaminase produced by the enterobacter ludwigii XL0404 is 484.48 U / L, and the enterobacter ludwigii XL0404 has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing siderophore and the like, has an inhibition effect on main root and stem disease pathogenic bacteria such as phytophthora nicotianae, rhizoctonia solani, rhizoctonia tectorum, fusarium oxysporum, fusarium solani and the like, and has an inhibition rate of 41.20-55.88%; the germination rate of the tobacco seeds is increased through treatment of the strain XL0404, and the root length is remarkably promoted; the root activity, the whole plant fresh weight, the overground fresh weight, the root fresh weight, the maximum leaf length and the maximum leaf width of the tobacco seedlings treated by the strain XL0404 with the bacterium content of 1 * 10 < 7 > cfu / mL in a seedling culture substrate are obviously higher than those of the tobacco seedlings treated by contrast treatment, and the strain XL0404 can be used as plant growth-promoting rhizobacteria and has a good application prospect in research and development of microbial fertilizers and biological prevention and control of tobacco rhizome diseases.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention belongs to the technical field of microorganisms, and discloses streptomycete Y309 and application thereof. The preservation number of the streptomyces sp. Y309 is GDMCC No: 64527, and the streptomyces sp. Y309 has relatively strong secondary metabolite synthesis capability and can be used for producing siderophore, IAA (Indoleacetic Acid) and the like. The strain Y309 also has the potential of plant growth promotion and disease control, and the strain Y309 has a relatively strong bacteriostatic effect on 12 pathogenic fungi such as fusarium oxysporum, rhizoctonia cerealis, monilinia fusarium and coracocystis fimbristipula as well as 7 pathogenic bacteria such as pseudomonas syringae lacrimation pathogenic variants and bacillus cereus, and has a relatively strong bacteriostatic effect on 12 pathogenic fungi such as fusarium oxysporum, rhizoctonia cerealis, monilinia fusarium and coracocystis fimbristipula. The strain Y309 has good prevention and treatment effects on wheat sharp eyespot, tomato gray mold and pepper fusarium wilt, fermentation liquor of the strain Y309 can remarkably promote wheat seed germination and seedling growth, agronomic characters such as the plant height, the dry weight and the leaf area in the corn seedling stage can be improved in different saline-alkali soil, and the strain Y309 has good application prospects.

According to the nano pesticide and the preparation method and application thereof, pH response type nano particles ZIF-90 serve as a carrier, azoxystrobin is loaded to serve as a medicine, and the nano pesticide is used for preventing and treating rice sheath blight disease caused by rhizoctonia solani. When the rhizoctonia solani infects rice, oxalic acid can be generated to reduce the pH around an infected area, so that the ZIF-90 carrier skeleton and the hyaluronic acid coating are decomposed to release azoxystrobin, and the rhizoctonia solani is inhibited in a targeted manner. It is further found that ZIF-90 and azoxystrobin have synergistic bacteriostatic activity, the EC50 value of azoxystrobin to rhizoctonia solani can be greatly reduced, after ZIF-90 carrier encapsulation and hyaluronic acid end capping, the photolysis stability of azoxystrobin can be improved, the adhesive force of azoxystrobin on rice leaves can be enhanced, and the ZIF-90 and azoxystrobin compound has important application in agricultural disease control.

The invention discloses a bacillus stutzeri MQ20YS04 and an application of the bacillus stutzeri MQ20YS04. The preservation number of the bacillus swezeyi MQ20YS04 disclosed by the invention is CCTCC (China Center for Type Culture Collection) NO: M 2024616, and the preservation number of the bacillus swezeyi MQ20YS04 disclosed by the invention is CCTCC NO: M 2024616. The strain has strong secondary metabolite synthesis capability, and can produce siderophore, IAA and the like. The strain MQ20YS04 also has the potential of plant growth promotion and disease control, and the strain MQ20YS04 has a relatively strong bacteriostatic effect on various pathogenic fungi such as botrytis cinerea, fusarium oxysporum, rhizoctonia solani and sclerotinia sclerotiorum, and has a very good control effect on pepper phytophthora blight and codonopsis pilosula root rot. In addition, the strain MQ20YS04 also has nitrogen fixation and phosphorus solubilization capabilities, and has a good application prospect.

The invention discloses an application of 3-phenyl propyl acetate in preparation of a medicine for preventing and treating fungal diseases of rice. Experiments show that the compound can effectively inhibit mycelial growth and conidia germination of magnaporthe oryzae, can interfere conidia of magnaporthe oryzae to form appressorium, has an obvious inhibition effect on magnaporthe oryzae, and also inhibits mycelial growth of rhizoctonia solani. The inventor also accidentally finds that the antibacterial effect of the pesticide can be promoted by adding a small amount of 3-phenyl propyl acetate into the pesticide, so that the small amount of 3-phenyl propyl acetate can be used as a pesticide synergist for preventing and treating the rice fungal diseases. Therefore, the 3-phenyl propyl acetate has good application value in the aspects of prevention and control of rice fungal diseases and drug development.

The invention belongs to the technical field of microorganisms, and particularly discloses trichoderma harzianum NBU-1 and application thereof. The Trichoderma harzianum NBU-1 is preserved in the Guangdong Microbial Culture Collection Center on March 26, 2025, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCCNO: 66053. The invention discloses trichoderma harzianum NBU-1 and application thereof, the trichoderma harzianum NBU-1 is applied to prevention and treatment of wheat sharp eyespot, and the activity of rhizoctonia cerealis R0301 and the growth of scab are effectively inhibited.

The invention discloses bacillus cereus L15, and relates to the technical field of microorganisms, the bacillus cereus L15 is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 2, 2025, and the preservation number is CGMCC No.33276. The bacillus cereus L15 has the advantages that the bacillus cereus L15 can be used for preparing the bacillus cereus L15; the new bacillus cereus L15 provided by the invention has obvious broad-spectrum antibacterial property, can have a good inhibition effect on rhizoctonia dinuclear, fusarium and phaseolus vulgaris, and can be used for preventing and treating plant root rot and stem rot caused by the fusarium, the rhizoctonia and the phaseolus vulgaris.

The invention discloses an efficient broad-spectrum biocontrol bacterium pseudomonas FM36 and application thereof. The wheat pseudomonas is a strain FM36, is classified and named as Pseudomonas siliginis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC NO.32868. The invention further discloses a preparation method of the high-efficiency broad-spectrum biocontrol bacterium pseudomonas FM36. The invention further discloses an application of the high-efficiency broad-spectrum biocontrol bacterium pseudomonas FM36, and the application of the high-efficiency broad-spectrum biocontrol bacterium pseudomonas FM36. The invention further provides application of the wheat pseudomonas FM36 strain in promoting tomato growth and preventing and treating plant viruses and pathogenic fungi. The wheat pseudomonas provided by the invention has a promoting effect on the growth and yield increase of tomatoes, and has obvious antagonism and obvious antagonism on pathogenic fungi such as tobacco mosaic virus (TMV), chilli vein mottle virus (ChiVMV), rhizoctonia solani, Magnaporthe oryzae, alternaria tenuissima, colletotrichum sp.sp.cubense, fusarium oxysporum f.sp.cubense and the like, and the wheat pseudomonas has an obvious antagonism effect on pathogenic fungi such as the tobacco mosaic virus (TMV), the chilli vein mottle virus (ChiVMV), the rhizoctonia solani, the Magnaporthe oryzae, the alternaria tenuissima, the colletotrichum sp.sp.cubense and the like.

The invention belongs to the technical field of agricultural microorganisms, and discloses an application of an RNA enzyme inhibitor guanosine 5 '-monophosphate (5'-GMP) in improving the disease resistance of plants. The invention discloses an application of an RNA enzyme inhibitor 5 '-GMP in prevention and treatment of soybean root rot caused by fusarium graminearum and rhizoctonia solani, soybean fusarium wilt caused by fusarium oxysporum and alfalfa root rot caused by fusarium oxysporum. Experiments prove that the 5 '-GMP can significantly improve the disease resistance of soybeans to fusarium graminearum, fusarium oxysporum and rhizoctonia solani and the disease resistance of medicago sativa to fusarium oxysporum, and in addition, the RNA enzyme inhibitor 5'-GMP can significantly promote the growth of soybeans, medicago sativa and rice.

The invention discloses an 8-bromine-5-substituted-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole derivative, and the 8-bromine-5-substituted-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole derivative is 8-bromine-5-(4-chlorophenethyl)-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole and / or 8-bromine-5-phenethyl-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole. The invention further discloses a preparation method of the 8-bromine-5-substituted-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole derivative. The 8-bromo-5-substituted-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole derivative provided by the invention has the effects of inhibiting rhizoctonia solani and promoting seed germination. The compound provided by the invention not only can be used for preventing and treating rice sheath blight disease through spraying treatment, but also can be used for preventing rice seeds from carrying bacteria in a seed soaking manner, so that the rice sheath blight disease is comprehensively prevented and controlled. Besides, rice seeds are soaked in a compound solution, germination of the rice seeds can be promoted, the root length, growth vigor and fresh weight indexes of the seeds are improved, and the compound has good application prospects and value in prevention and treatment of the rice sheath blight disease.

The present invention relates to the field of microorganisms, and particularly to a Bacillus amyloliquefaciens BZWQ833, a microbial agent and its application. A Bacillus amyloliquefaciens was screened from the rhizosphere soil of a potato common scab field with continuous cropping for many years, named Bacillus amyloliquefaciens BZWQ833 and subjected to biological preservation. This bacterium is a Gram-positive bacterium that can produce spores and can produce protease, cellulase and pectinase. The present invention first discovers that Bacillus amyloliquefaciens BZWQ833 can inhibit the growth and incidence of Streptomyces scabies, Rhizoctonia solani, Fusarium oxysporum and Fusarium solani, and the antibacterial effect is stable. Moreover, through greenhouse pot experiments and field control experiments, it is verified that Bacillus amyloliquefaciens BZWQ833 has a significant control effect on various plant soil-borne diseases (common scab, powdery scab and fruit rot), providing a new biological control resource for the biological control of plant soil-borne diseases.

The present invention discloses an actinomycete with antagonistic effect against the pathogen of cucumber damping-off and its application. The actinomycete is Streptomyces aureusensis Q1C-44, which has antagonistic effect against Rhizoctonia solani of cucumber, can effectively inhibit the hyphal elongation of the pathogenic fungus, and can be used to control damping-off caused by Rhizoctonia solani of cucumber.

The application provides a streptomyces LZZY-S3 and application thereof, and belongs to the field of microorganisms.The streptomyces LZZY-S3 provided by the application enriches strains for biological control, and realizes the dual functions of plant resistance to pathogenic bacteria and plant promotion.The streptomyces LZZY-S3 has the function of inhibiting fungi in a wide range, and can be applied to plant resistance to pathogenic bacteria, and the pathogenic bacteria include Exobasidium vexans, Botryosphaeria spp., Gibberella, Fusarium oxysporum, Pythium, Fusarium graminearum, Didymella, Fusarium pseudograminearum, Trichothecium roseum, Coniella diplodiella, Colletotrichum graminicola or Rhizoctonia cerealis, wherein the inhibition rate of Exobasidium vexans reaches 91.2%.The streptomyces LZZY-S3 can also be applied to plant promotion, preparation of nitrogen-fixing bacterial agents and preparation of ACC deaminase bacterial agents and the like.

The present application discloses a highly effective and broad-spectrum biocontrol bacterium Pseudomonas FM36 and its application. The Pseudomonas aestivum is strain FM36, which is classified and named Pseudomonas aestivum Pseudomonas rye , deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, with the deposit number of CGMCC NO.32868. The present application also provides the use of the above-mentioned Pseudomonas aestivum FM36 strain in promoting tomato growth, preventing and controlling plant viruses and pathogenic fungi. The Pseudomonas aestivum provided in the present application has a promoting effect on tomato growth and yield increase, and has an effect on tobacco mosaic virus (TMV) and red pepper vein mottle virus (ChiVMV), as well as Rhizoctonia solani ( Rhizoctonia solani )、Pyricularia grisea( Magnaporthe rice )、Alternaria tenuifolia( Alternaria tenuissima )、White wax spiny spore ( Colletotrichum spaethianum ), Fusarium spp. Cuban type ( Fusarium oxysporum f.sp. from Cuba ) and other pathogenic fungi showed obvious antagonistic and inhibitory effects.

The invention discloses a soybean phomopsis longicolla resistance gene and application thereof, and belongs to the field of genetic engineering, the gene is Psd18.1, and the resistance of soybeans to the soybean phomopsis longicolla can be improved by knocking out or silencing the soybean Psd18.1 gene. It is proved that the Psd18.1 negatively regulates and controls the resistance of the soybeans to the PSD. The invention provides a new choice for preventing and treating the soybean phomopsis seed rot and cultivating disease-resistant varieties.

The present application belongs to the technical field of orchid disease prevention and treatment, and particularly relates to a fungicidal composition for preventing and treating orchid root rot. The fungicidal composition for preventing and treating orchid root rot comprises active ingredients of nerol and compound a, wherein the compound a is at least one selected from prochloraz, difenoconazole and carbendazim. When nerol is compounded with prochloraz, difenoconazole and carbendazim, the synergistic effect is shown by the co-toxicity coefficient (CTC) value of the compound combination to the pathogenic fungus of Rhizoctonia solani being greater than 120, which indicates that the compound combination can improve the prevention and treatment effect on orchid root rot. In addition, the fungicidal composition contains two effective ingredients, which can delay the generation and development of pathogenic fungi resistance and overcome the problems existing in long-term use of single component fungicides. In summary, the fungicidal composition can be used for developing fungicides for preventing and treating orchid root rot.

The invention discloses a virens and white beard fungus strain and application thereof, and belongs to the technical field of biology. The preservation number of the white beard virens SJ-50F is CCTCC (China Center For Type Culture Collection) NO: M 20251911, and the white beard virens SJ-50F has a remarkable inhibition effect on a rice sheath blight disease pathogenic bacterium rhizoctonia solani YM-Q-7. According to the invention, an in-vitro confrontation culture and potting verification method is adopted, which proves that the virens SJ-50F can inhibit the growth of germs, a plate confrontation experiment shows that the hypha growth inhibition rate of YM-Q-7 reaches 82.36%, and the hypha growth inhibition rate of fermentation supernate of the virens SJ-50F to YM-Q-7 reaches 90.79%.

The present invention belongs to the field of microbial technology and discloses Streptomyces sp. Y309 and its application. The Streptomyces Streptomyces sp. Y309, with the preservation number GDMCC No: 64527, has a strong ability to synthesize secondary metabolites and can produce siderophores, IAA, etc. This strain also has the potential for plant growth promotion and disease control. Strain Y309 has strong antibacterial effects on 12 pathogenic fungi such as Fusarium oxysporum, Rhizoctonia cerealis, Monilinia fructicola, and Ceratocystis fimbriata, as well as 7 pathogenic bacteria such as Pseudomonas syringae pv. lachrymans and Bacillus cereus. It has good control effects on wheat sharp eyespot, tomato gray mold, and pepper fusarium wilt. The fermentation broth of strain Y309 can significantly promote the germination of wheat seeds and the growth of seedlings, and can improve the agronomic traits such as plant height, dry weight, and leaf area of maize seedlings in different saline-alkali soils, showing good application prospects.

The present application provides a kind of compound biocontrol agent and its preparation method and application technology, and it is composed of 1000ml of fermentation mixed liquor of multiple strains of biocontrol bacteria mixed in equal volume ratio and 0.5%-1.5% stabilizer with complementary mechanism and target.The fermentation concentrated solution of multiple strains of bacteria is prepared in fermentation medium to obtain the present application, which is used for preventing and controlling fusarium, pythium, phytophthora, rhizoctonia solani, ralstonia solanacearum, erwinia carotovora, streptomyces scabiei, xanthomonas campestris and other plant pathogenic bacteria and nematodes, which can inhabit in soil for a long time and spread through soil, and one or several of them can cause plant soil-borne diseases.The preparation process of the present application is not affected by too many chemical and physical factors, the activity of live bacteria and metabolites is high, the average control effect is 78%-85%, the application effect is good;The production process is simple, energy and auxiliary materials are saved, and the production cost is low.

The invention discloses streptomyces alboflavus (with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35588) for preventing and treating muskmelon fusarium wilt, and belongs to the field of biological prevention and treatment. The strain is high in sporulation quantity and strong in colonization capacity, pathogenic bacteria are inhibited and growth is promoted through antagonism, secondary metabolites, induction system resistance and other mechanisms, the potting control effect reaches 86.3%, and the effect lasts for 21 days. The streptomyces albidoflavus not only can prevent and treat fusarium wilt of muskmelons, but also has a broad-spectrum inhibition effect on fusarium wilt of cucumbers, watermelons and tomatoes and soil-borne diseases such as pepper phytophthora blight and rhizoctonia solani, and can improve rhizosphere microecology and promote reproduction of effective microbial communities. The biocontrol microbial agent is a pure biological agent, is pollution-free and nuisanceless, and can remarkably reduce the use of chemical pesticides, relieve continuous cropping obstacles and promote green development of the watermelon and melon industry.

The invention relates to a method of infestation of a plant by phytopathogenic microorganisms of the Fusarium genus, the Rhizoctonia genus or the Alternaria genus, which comprises applying, in any desired sequence or simultaneously, on the plant, the locus thereof or its propagation material, a combination or composition comprising as component (A), a compound of formula (A-1) and, as component (B), a compound of formula (B-1). Also disclosed are compositions suitable for use in the invention.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

This invention belongs to the field of microbial control technology, specifically relating to a biocontrol bacterium for soybean root rot, a microbial agent, its preparation method, and its application, which can improve soybean yield and oil content. The biocontrol bacterium is *Alcaligenes faecalis* (Factophytes). Alcaligenes faecalis A new strain, named GY16, was deposited at the China Center for Type Culture Collection (CCTCC) on November 12, 2025, with accession number CCTCC M 20252536. This alkali-producing bacterium, GY16, exhibits a specific affinity for soybean, enabling it to colonize soybean roots and effectively control soybean root rot. GY16 also promotes soybean plant growth, increases soybean yield, and improves seed oil content. Inoculants prepared using GY16 can replace pesticides, offering a green, environmentally friendly, and pollution-free alternative that positively contributes to building a sustainable farmland ecosystem.