40 results about "Rhizoctonia" patented technology

The invention discloses a soybean phomopsis longicolla resistance gene and application thereof, and belongs to the field of genetic engineering, the gene is Psd18.1, and the resistance of soybeans to the soybean phomopsis longicolla can be improved by knocking out or silencing the soybean Psd18.1 gene. It is proved that the Psd18.1 negatively regulates and controls the resistance of the soybeans to the PSD. The invention provides a new choice for preventing and treating the soybean phomopsis seed rot and cultivating disease-resistant varieties.

The present application belongs to the technical field of orchid disease prevention and treatment, and particularly relates to a fungicidal composition for preventing and treating orchid root rot. The fungicidal composition for preventing and treating orchid root rot comprises active ingredients of nerol and compound a, wherein the compound a is at least one selected from prochloraz, difenoconazole and carbendazim. When nerol is compounded with prochloraz, difenoconazole and carbendazim, the synergistic effect is shown by the co-toxicity coefficient (CTC) value of the compound combination to the pathogenic fungus of Rhizoctonia solani being greater than 120, which indicates that the compound combination can improve the prevention and treatment effect on orchid root rot. In addition, the fungicidal composition contains two effective ingredients, which can delay the generation and development of pathogenic fungi resistance and overcome the problems existing in long-term use of single component fungicides. In summary, the fungicidal composition can be used for developing fungicides for preventing and treating orchid root rot.

The invention discloses a virens and white beard fungus strain and application thereof, and belongs to the technical field of biology. The preservation number of the white beard virens SJ-50F is CCTCC (China Center For Type Culture Collection) NO: M 20251911, and the white beard virens SJ-50F has a remarkable inhibition effect on a rice sheath blight disease pathogenic bacterium rhizoctonia solani YM-Q-7. According to the invention, an in-vitro confrontation culture and potting verification method is adopted, which proves that the virens SJ-50F can inhibit the growth of germs, a plate confrontation experiment shows that the hypha growth inhibition rate of YM-Q-7 reaches 82.36%, and the hypha growth inhibition rate of fermentation supernate of the virens SJ-50F to YM-Q-7 reaches 90.79%.

The present application provides a kind of compound biocontrol agent and its preparation method and application technology, and it is composed of 1000ml of fermentation mixed liquor of multiple strains of biocontrol bacteria mixed in equal volume ratio and 0.5%-1.5% stabilizer with complementary mechanism and target.The fermentation concentrated solution of multiple strains of bacteria is prepared in fermentation medium to obtain the present application, which is used for preventing and controlling fusarium, pythium, phytophthora, rhizoctonia solani, ralstonia solanacearum, erwinia carotovora, streptomyces scabiei, xanthomonas campestris and other plant pathogenic bacteria and nematodes, which can inhabit in soil for a long time and spread through soil, and one or several of them can cause plant soil-borne diseases.The preparation process of the present application is not affected by too many chemical and physical factors, the activity of live bacteria and metabolites is high, the average control effect is 78%-85%, the application effect is good;The production process is simple, energy and auxiliary materials are saved, and the production cost is low.

The invention discloses streptomyces alboflavus (with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35588) for preventing and treating muskmelon fusarium wilt, and belongs to the field of biological prevention and treatment. The strain is high in sporulation quantity and strong in colonization capacity, pathogenic bacteria are inhibited and growth is promoted through antagonism, secondary metabolites, induction system resistance and other mechanisms, the potting control effect reaches 86.3%, and the effect lasts for 21 days. The streptomyces albidoflavus not only can prevent and treat fusarium wilt of muskmelons, but also has a broad-spectrum inhibition effect on fusarium wilt of cucumbers, watermelons and tomatoes and soil-borne diseases such as pepper phytophthora blight and rhizoctonia solani, and can improve rhizosphere microecology and promote reproduction of effective microbial communities. The biocontrol microbial agent is a pure biological agent, is pollution-free and nuisanceless, and can remarkably reduce the use of chemical pesticides, relieve continuous cropping obstacles and promote green development of the watermelon and melon industry.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

This invention belongs to the field of microbial control technology, specifically relating to a biocontrol bacterium for soybean root rot, a microbial agent, its preparation method, and its application, which can improve soybean yield and oil content. The biocontrol bacterium is *Alcaligenes faecalis* (Factophytes). Alcaligenes faecalis A new strain, named GY16, was deposited at the China Center for Type Culture Collection (CCTCC) on November 12, 2025, with accession number CCTCC M 20252536. This alkali-producing bacterium, GY16, exhibits a specific affinity for soybean, enabling it to colonize soybean roots and effectively control soybean root rot. GY16 also promotes soybean plant growth, increases soybean yield, and improves seed oil content. Inoculants prepared using GY16 can replace pesticides, offering a green, environmentally friendly, and pollution-free alternative that positively contributes to building a sustainable farmland ecosystem.

The invention relates to the technical field of bacteriostatic preparations, in particular to mugwort essential oil as well as a preparation method and application thereof. Mixing mugwort with a sodium chloride solution, and soaking to obtain a soaked material; the material-liquid ratio of the soaked material is 4-12 mL / g; the mass percentage content of sodium chloride in the sodium chloride solution is 5-20%; the soaking time is 1-4 hours; distilling the soaked material, standing and layering distillate, and collecting upper-layer essential oil to obtain artemisia argyi essential oil; the distillation time is 1 to 5 hours. The mugwort essential oil provided by the invention has a relatively good inhibition effect on the growth of common plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum and fusarium oxysporum, so that the mugwort essential oil is used as a biological bactericide in future agricultural production, and the effect of preventing and treating diseases can be achieved to a certain extent.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas aeruginosa and application thereof. The invention discloses Pseudomonas aeruginosa R5-2, and the number of the Pseudomonas aeruginosa R5-2 is CGMCC (China General Microbiological Culture Collection Center) No.34796. The invention further discloses a preparation method of the Pseudomonas aeruginosa R5-2. The strain can secrete various active substances such as siderophores and chitinase, and can effectively inhibit growth of various pathogenic bacteria such as fusarium oxysporum, verticillium dahliae, rhizoctonia solani and fusarium graminearum. Experiments show that a fungicide containing the strain has a remarkable prevention and treatment effect on lycium barbarum root rot and tomato fusarium wilt caused by fusarium oxysporum. The invention provides a novel and environment-friendly microbial resource for preventing and treating soil-borne fungal diseases of crops such as medlar, tomato and the like, and has important application value.

The invention relates to the technical field of biology, in particular to a defensive protein derived from konjak and having broad-spectrum antibacterial activity, a coding gene and application of the defensive protein and the coding gene. According to the invention, the defensive protein is obtained from konjak for the first time, and the defensive protein has an obvious inhibition effect on multiple fungi (rhizoctonia solani, fusarium oxysporum, fusarium aromatic, fusarium solani and phytophthora nicotianae) and bacteria (bacillus cereus and ralstonia solanacearum). The defensive protein has wide application prospects in the fields of food, hygienic products, cosmetics, biopesticides, biological feed additives or natural food preservatives and the like.

The invention provides application of an OsNAC15 gene in improving resistance of rice to rhizoctonia solani, and belongs to the technical field of biology. The invention provides application of a reagent for inhibiting the expression of the OsNAC15 gene in improving the resistance of rice to rhizoctonia solani or improving the resistance of rice to sheath blight, provides negative regulation of the resistance of the rice to rhizoctonia solani by the OsNAC15 for the first time, and supplements the function of the OsNAC15 in interaction of the rice and rhizoctonia solani and a disease-resistant pathway for regulating a PR gene by an NAC transcription factor; and a new thought is provided for research on a rice-rhizoctonia solani interaction mechanism.

The application discloses a Rosa chinensis disease prevention and growth promotion bacterium LX6 strain and application thereof, and belongs to the field of microorganism strains and application thereof. The Rosa chinensis disease prevention and growth promotion bacterium LX6 is preserved in the China General Microbiological Culture Collection Center on May 16, 2024, and has a preservation number of CGMCC No. 30657 and a classification name of Pseudomonas fluorescens. The bacterium strain has the ability to resist high-concentration salt ions and various heavy metal ions. The bacterium strain can inhibit the growth of a Rosa chinensis black spot pathogen, i.e., Discosia rosea, and can also antagonize the growth of plant pathogenic fungi, i.e., Colletotrichum gloeosporioides, Rhizoctonia solani, Fusarium oxysporum and Fusarium equiseti, so as to prevent and treat the Rosa chinensis black spot and powdery mildew, and promote the growth and flowering of the Rosa chinensis. The application provides a fine biocontrol strain for preventing and treating the diseases of the Rosa chinensis and promoting the growth of the Rosa chinensis, and has important significance for green prevention and control technology of the diseases of the Rosa chinensis and sustainable development of the Rosa chinensis industry.

The application provides a method for screening soybean Cercospora sojina blight response genes based on transcriptome analysis, which comprises the following steps: selecting pods of soybean Cercospora sojina blight infected plants and healthy plants planted in the field, performing transcriptome sequencing, screening differential expression genes of soybean responding to Cercospora sojina blight, analyzing biological pathways of the differential expression genes in the Cercospora sojina blight infection process, performing protein interaction analysis, screening interaction core genes, comparing sequences, performing transcript from scratch assembly, analyzing differential variable splicing events and exon usage differences through integration analysis, analyzing post-transcriptional regulation network dynamic characteristics of key candidate genes of soybean responding to Cercospora sojina blight, and screening Cercospora sojina blight response genes of soybean. Four Cercospora sojina blight response genes of soybean are obtained, and expression characteristics in different soybean tissues are analyzed, which provides a theoretical basis for soybean Cercospora sojina blight resistance breeding.

The invention relates to a novel acridinyl-containing tetrahydronaphthalene spiro isoxazoline compound, a preparation method thereof and application of the novel acridinyl-containing tetrahydronaphthalene spiro isoxazoline compound as an antifungal drug. The novel acridinyl-containing tetrahydronaphthalene spiroisoxazoline compound has an inhibition effect on parasite sphaerospora, botrytis cinerea, rhizoctonia solani, fusarium graminearum and candida albicans in the antifungal activity of the novel acridinyl-containing tetrahydronaphthalene spiroisoxazoline compound. An antifungal biological activity result shows that the compound shows remarkable antifungal activity, has comprehensive antifungal characteristics and is determined as a broad-spectrum antifungal candidate, the EC50 value of the compound to parasitic powdery spores is superior to that of a positive control drug chlorothalonil, and the compound shows about 12 times and 25 times of titer advantages compared with famoxadone and carbendazim respectively. The spiro structure and multiple active groups of the compound can significantly improve the antifungal activity of the compound, and in addition, the compound has protection and treatment effects on powdery parasitic spore infection in corn, which shows that the compound has agricultural application potential.

The present invention relates to a microbial strain having superior fungicidal and / or nematicidal activity, as well as its progeny and derivatives, compositions comprising the same, and methods of using the same, inter alia in agriculture. The strain, as well as its progeny and derivatives, is capable of increased fungicidal activity against at least one fungi selected from the group consisting of: Botrytis, Fusarium, and Rhizoctonia when compared to Bacillus velezensis strain GB03.

The invention relates to application of trichoderma harzianum to prevention and treatment of potato black scurf and scab. The trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.40017 can well inhibit rhizoctonia solani, so that the effect of preventing and treating potato black scurf is achieved; trichoderma harzianum with the preservation number of CGMCC No.40017 can also inhibit streptomycete, such as streptomyces scabies, streptomyces scabies and streptomyces griseus, so that the effect of preventing and treating potato scab is achieved, the strain or the microbial agent containing the strain is applied to the planting process of potatoes, potato black scurf and scab can be effectively prevented and treated, and the effect of preventing and treating potato scab is achieved. The method has important significance in agricultural production.

The application discloses a bacillus velezensis BZWQ55, a microbial agent and application thereof, and belongs to the field of microorganisms. The bacillus velezensis BZWQ55 is screened from rhizosphere soil of potato scab and has a preservation number of CGMCC No. 36805. Experimental results show that the bacillus velezensis BZWQ55 has the abilities of producing cellulase, protease, amylase and iron carrier, and has the abilities of phosphorus and potassium solubilization and nitrogen fixation, and can effectively promote plant growth; the bacillus velezensis BZWQ55 can also stably inhibit the growth of Fusarium solani, Fusarium oxysporum, Alternaria solani, Streptomyces scabrisporus, Rhizoctonia solani and Neocosmospora vasinfecta, and has a significant prevention effect on multiple soil-borne diseases such as potato scab, sweet potato root rot, potato scab and peanut fruit rot. The application provides a new strain resource for biological control of plant soil-borne diseases and promotion of plant growth.

The invention discloses an application of any compound of an Oxoapophine alkaloid derivative in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative shows certain inhibitory activity on two plant pathogenic bacteria such as rice bacterial blight and citrus canker pathogen, and five plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, magnaporthe grisea and wheat stem rot; the compound has a good inhibition effect on citrus canker pathogen and sclerotinia sclerotiorum. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The invention discloses a composition containing pydiflumetofen and epoxiconazole as well as a preparation method and application of the composition, and belongs to the technical field of pesticides. The bactericidal composition disclosed by the invention takes the pydiflumetofen and the epoxiconazole as main active components, has a synergistic effect, has a wider bactericidal spectrum, particularly has an excellent synergistic prevention and treatment effect on diseases caused by fusarium and rhizoctonia, and can effectively delay the generation of drug resistance of pathogenic bacteria, improve the prevention and treatment effect and prolong the lasting period; besides, tetradecyl methyl dihydroxyethyl ammonium bromide is introduced into the modified bentonite, the bactericidal effect can be enhanced, organic silicon is introduced into the surface of the modified silicon dioxide, the modified silicon dioxide can also be adsorbed on the surfaces of bacteria, the seepage force to cell walls is enhanced, and the bactericidal effect is further improved. On the whole, the dispersion stability is excellent, particle aggregation and caking are prevented, the uniformity and stability of the product in the storage and application process are guaranteed, and the sterilization effect is more excellent.

The invention provides trichoderma asperellum SWU Y1-45 and application thereof, and relates to the technical field of biological control. Comprising trichoderma asperellum SWU Y1-45, the classification name of the trichoderma asperellum is Trichoderma asperellum, the trichoderma asperellum SWU is preserved in China General Microbiological Culture Collection Center on May 22, 2025, the preservation number of the trichoderma asperellum SWU is CGMCC No.41950, and the preservation address of the trichoderma asperellum SWU is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The compound has a good inhibition effect on plant pathogenic fungi such as rhizoctonia binuclear, fusarium, phaseolus vulgaris, colletotrichum gloeosporioides and trichosporon actinosporium, and can realize efficient prevention and treatment on sweet osmanthus root rot, ginkgo basal rot, loquat anthracnose and loquat stem rot. The invention also provides a derivative of trichoderma asperellum SWU Y1-45, which is applied to practice, can also effectively realize prevention and treatment of sweet osmanthus root rot, ginkgo basal rot, loquat anthracnose and loquat stem rot, and has a wide application prospect in agricultural green production.

The present application provides a kind of plant pathogenic soil biological control agent and its preparation method and application technology, which is composed of multiple different genera of biocontrol strains and their metabolite concentrated solid 70-90% and auxiliary materials 10-30% with complementary mechanism and target.The fermentation concentrated liquid of multiple strains is mixed with carrier, and then spray dried to obtain the compound bacteria fermentation liquid concentrated solid;Then it is mixed with auxiliary materials to granulate to obtain the present application.The present application is used for the prevention and control of plant diseases caused by one or several of fusarium, pythium, pathogenic streptomyces, rhizoctonia solani, raoultella, erwinia carotovora and other plant pathogenic bacteria and nematodes that can survive in soil and its pathogenic soil.The present application effectively improves the microecological environment of pathogenic soil, induces plant disease resistance effect, constructs a good plant soil ecosystem and promotes plant growth and development.

This invention belongs to the field of agricultural microbiology technology, specifically relating to a microbacterium strain and its application in plant disease control. This invention discloses a microbacterium strain screened from wolfberry roots, named... Microbacterium sp. R5-1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 34797, has the 16S rDNA sequence shown in SEQ ID NO:1. It secretes siderophores, cellulase, and protease. Experimental results demonstrate that this strain exhibits inhibitory activity against the growth of *Fusarium oxysporum*, *Rhizoctonia solani*, and *Verticillium dahliae*, and shows significant control efficacy against soil-borne fungal diseases of plants caused by these fungi. This invention provides an important microbial resource for the green control of soil-borne fungal diseases of plants.

This invention discloses a smart pH-responsive sulfur-based nanocomposite antibacterial material and its application in the removal of soil pathogenic microorganisms. The material is produced by in-situ generation of nano-sulfur from nanocellulose through sodium periodate oxidation and L-lysine grafting modification, followed by Zn... 2+ Zn was prepared by coating with tannins and spray drying. Under neutral conditions in healthy soil, Zn... 2+ The tannic acid shell provides stable sealing, with a sulfur release rate of <4.2% over 48 hours. In the acidic microenvironment (pH 5.0-6.0) generated by pathogen metabolism, the shell rapidly disintegrates, resulting in a release rate >85% over 48 hours, achieving a smart switch effect of healthy soil preservation followed by lesion-triggered release. This material exhibits broad-spectrum, rapid, and long-lasting killing effects against soil-borne pathogens such as Ralstonia solanacearum, Fusarium oxysporum, and Rhizoctonia solani. It is also environmentally friendly, scalable, and suitable for targeted removal of soil pathogens.

The application of leidinmycin compounds in inhibiting plant pathogenic fungi and preventing and treating plant diseases belongs to the technical field of pesticide fungicides. In order to screen leidinmycin compounds capable of being used for preventing and treating plant diseases caused by plant pathogenic fungi or plant pathogenic oomycetes, five kinds of leidinmycin compounds are used for inhibiting plant pathogenic fungi and preventing and treating plant diseases. The leidinmycin compounds have strong inhibitory effect on plant pathogenic fungi, including the growth of fungal hyphae in Sclerotinia, Rhizoctonia, Elsinoe, Botrytis and Alternaria and the growth of oomycete hyphae in Pythium, Peronospora and Pseudomonas. In addition, the leidinmycin compounds have good prevention and treatment effect on plant diseases caused by the above plant pathogenic fungi and plant pathogenic oomycetes, and have no toxic side effect on plants, and can be developed and applied as effective components of pesticides or plant pathogenic fungicides.

The invention belongs to the technical field of biological control, and particularly relates to an enterobacter ludwigii SLGJ4, a fungicide and application of the enterobacter ludwigii SLGJ4, the preservation number of the enterobacter ludwigii SLGJ4 is CCTCC No: M 20251540, the bacterial strain has broad-spectrum antibacterial activity, and the antibacterial rates of the bacterial strain on Colletotrichum gloeosporioides of pomegranate, Colletotrichum truncatum of pepper, rhizoctonia cerealis of wheat and Botryis cinerea of tomato are 55.48%, 51.03%, 48.67% and 47.15% respectively; the control effect on pomegranate fruit anthracnose is 60.99%, the enzyme activity of defensive enzyme POD of pomegranate fruits is remarkably improved, the activity of PPO is inhibited, and enzymatic browning of the pomegranate fruits is delayed; after colletotrichum gloeosporioides hyphae are treated by the strain SLGJ4, the growth of the hyphae is obviously inhibited In addition, the strain can also cause accumulation of intracellular active oxygen of colletotrichum gloeosporioides, destroy plasma membrane integrity, has a good control effect on pomegranate fruit anthracnose, and provides a theoretical basis for development of pomegranate biocontrol inoculants and preservatives.

The invention provides a microbial agent for preventing and treating soybean soil-borne diseases and application of the microbial agent, and belongs to the technical field of agricultural biology. The microbial agent is prepared by compounding a bacterial strain RHEr1 (Enterobacter rogengenkampii), a bacterial strain RHCc2 (Chryseobacterium cucumerii), a bacterial strain RHPc03 (Pseudomonas chlororaphis) and a bacterial strain RHBs9 (Bacillus subtilis), and the ratio of viable counts of the bacterial strains is (20 to 28): (22 to 30): (18 to 24): (5 to 9). The microbial agent effectively prevents and treats soybean root rot caused by fusarium, rhizoctonia and the like through multiple antagonism, nutrition competition and an induction system resistance mechanism, has the functions of nitrogen fixation, phosphate solubilization and auxin secretion, and remarkably promotes the growth of soybean plants. The preparation is environment-friendly, has no chemical residue, is not easy to cause drug resistance, and is suitable for green and sustainable soybean production.

The invention discloses an allylamine antifungal agent and application of the allylamine antifungal agent. The allylamine antifungal agent can be used for preventing and treating diseases caused by plant pathogenic fungi such as rhizoctonia solani, fusarium graminearum, botrytis cinerea and the like. The CCXN-19-001 disclosed by the invention belongs to squalene epoxidase inhibition type allylamine antifungal agents, and the core action mechanism of the CCXN-19-001 is to inhibit biosynthesis of fungal cell membranes. The antibacterial agent with the action mechanism is not applied to agriculture. The problem that the resistance of the current agricultural traditional antibacterial agent is improved year by year can be well solved.

The present application belongs to the field of agricultural microorganism technology, and particularly relates to a Pseudomonas aeruginosa strain and application thereof Pseudomonas aeruginosa R5‑2, and the strain number is CGMCC No.34796.The strain can secrete iron carrier, chitinase and other active substances, and can effectively inhibit the growth of multiple pathogenic fungi such as Fusarium oxysporum, Verticillium dahliae, Rhizoctonia solani and Fusarium graminearum.The experiment shows that the fungicide containing the strain has a significant prevention and treatment effect on the root rot of wolfberry caused by Fusarium oxysporum and the tomato wilt.The present application provides a new, environment-friendly microbial resource for preventing and treating soil-borne fungal diseases of wolfberry, tomato and other crops, and has important application value.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a microbacterium strain and application thereof in plant disease control. The invention discloses a microbacterium obtained by screening from a wolfberry root system, the microbacterium is named as Microbacterium sp. R5-1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34797. The invention further discloses a preparation method of the microbacterium. The 16S rDNA sequence of the strain is as shown in SEQ ID NO: 1, and the strain can secrete and produce siderophores, cellulase and protease. Experimental results prove that the strain has the activity of inhibiting growth of fusarium oxysporum, rhizoctonia solani and verticillium dahliae and has a remarkable control effect on plant soil-borne fungal diseases caused by the fusarium oxysporum, the rhizoctonia solani and the verticillium dahliae. The invention provides an important microbial resource for green prevention and treatment of plant soil fungal diseases.

The application discloses a Trichoderma guepinii GUDJ16 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Trichoderma guepinii GUDJ16 screened by the application is CCTCC NO: M 20252004. The Trichoderma guepinii GUDJ16 has a prevention and treatment effect on kiwi soft rot, and the inhibition rate of the Trichoderma guepinii GUDJ16 on Botryosphaeria dothidea can reach 77.15%. The Trichoderma guepinii GUDJ16 also has an inhibition effect on Fusarium solani, Fusarium graminearum, Fusarium asiaticum, Rhizoctonia solani, Sclerotium rolfsii, Colletotrichum musae, Passalora fulva and Alternaria tenuissima. It is found through disease prevention and treatment effect verification on in-vitro fruits infected with kiwi soft rot that the prevention and treatment effect of the Trichoderma guepinii GUDJ16 on kiwi soft rot can reach 61.26% after treatment, and therefore the Trichoderma guepinii GUDJ16 has high application value in the field of kiwi soft rot prevention and treatment.