673 results about "Fusarium" patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention relates to a cultivation nutrient medium and a preparation method thereof. According to the nutrient medium, peat is completely abandoned, the coco coir, the straw and the biochar are combined to reduce ecological burden, the nutrient medium is an environment-friendly medium, the biochar fixes carbon and reduces emission, and the soil structure can be improved after long-term use; vermiculite improves the water-holding capacity, perlite and straw form a stable pore structure, and the ventilation porosity reaches 25% or above. Wormcast and a microbial agent synergistically release nitrogen, phosphorus and potassium, and the fertilizer efficiency is lasting. The microbial agent can inhibit soil-borne diseases such as fusarium and reduce the death rate of cultivated seedlings. The biochar adsorbs salt and toxins, and the EC value is stabilized at 1.2-1.8 mS / cm. The cost of the selected raw materials is reduced by 35% compared with that of a traditional substrate, and the substrate is suitable for large-scale production, suitable for vegetable seedling culture, potted flowers, organic farms and soilless culture systems, and especially suitable for saline-alkali soil or continuous cropping obstacle areas.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention relates to the technical field of environmental microorganisms, in particular to arthrobacterium Arth.R8 and application thereof, and the preservation number of the arthrobacterium Arth.R8 is GDMCC No.63567. The arthrobacter Arth.R8 has the effects of fixing nitrogen, dissolving organic phosphorus, promoting growth and promoting nodulation. The arthrobacter Arth.R8 disclosed by the invention can be used for promoting absorption and utilization of nutrient substances such as nitrogen and phosphorus in a plant growth process, inhibiting growth of fusarium graminearum and promoting plant growth, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention provides a fusarium specific gene segment and application of the fusarium specific gene segment in rapid detection of fusarium species. In particular, the present invention provides a fusarium specific segment. Furthermore, the invention also provides a primer pair for detecting fusarium species, and the primer pair is used for amplifying the fusarium specific segment disclosed by the invention. Furthermore, the invention also provides an application of the fusarium specific segment or the primer pair provided by the invention in detecting fusarium species. Through the fusarium specific section, different species in fusarium can be accurately distinguished, and the fusarium specific section can be applied to the field of plant quarantine to realize rapid and accurate detection of fusarium species.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention belongs to the technical field of agricultural biological control, and particularly relates to bacillus velezensis, a biological preparation and application of the bacillus velezensis in plant disease prevention and growth promotion. The bacillus velezensis is preserved in the China Center for Type Culture Collection, the preservation number of the bacillus velezensis is CCTCC NO: M 2025613, and the bacillus velezensis is classified and named as bacillus velezensis B54. Experiments show that the bacillus velezensis and the biological preparation thereof provided by the invention have broad-spectrum antagonistic activity, can effectively inhibit growth of pathogenic bacteria such as pythium spp and fusarium spp, induce plants to generate immune response, remarkably reduce the incidence rate of root rot and blight of the plants, can also generate plant growth stimulants, improve the development of plant root systems, improve the growth of the plant root systems and promote the growth of the plant root systems. The crop yield and quality are improved.

The invention discloses application of a TpGST gene in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. According to the application of the TpGST gene disclosed by the invention in regulating and controlling the ear rot and stem rot resistance of maize fusarium graminearum, the nucleotide sequence of the TpGST gene is shown as SEQ ID NO. 1. Fusarium graminearum is inoculated to ears and stems of an overexpressed TpGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic line is improved; fusarium graminearum is inoculated to ears and stems of the corn hybrid agricultural large 3138 overexpression TpGST transgenic line, and the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic hybrid is improved.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention belongs to the field of plant endophytic fungus secondary metabolites, particularly relates to a secondary metabolite of fennel endophytic fungus fusarium oxysporum as well as a preparation method and application thereof, and provides a fennel endophytic fungus secondary metabolite with structures as shown in compounds 1-8. Wherein the compound 2 and the compound 7 have inhibitory activity on staphylococcus aureus, escherichia coli, bacillus cereus, bacillus subtilis, candida albicans and shigella, and have a certain inhibitory effect on cervical cancer Hela cell strains.

The invention discloses beneficial fusarium oxysporum and application thereof in relieving apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The biological preservation number of the fusarium oxysporum FoB1 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.42195. The fusarium oxysporum FoB1 disclosed by the invention is beneficial fusarium oxysporum, and has the advantages of excellent biocontrol function, strong environmental adaptability and high affinity with a host. By optimizing the preparation process of the microbial inoculant, the activity and stability of the inoculant are remarkably improved, and a high-quality bacterium source is provided for large-scale production. On the basis, a simple and convenient bacterium-bearing nursery stock application technology is developed, a novel efficient biological prevention and control way is provided for relieving apple continuous cropping obstacles, and important technical support is provided for promoting green and sustainable development of the fruit tree industry.

The invention belongs to the technical field of crop disease prevention and treatment, and particularly relates to an anti-fusarium graminearum polypeptide SmAFP derived from Sitodiplosis mosellana, a nucleotide sequence coded by the same and application of the anti-fusarium graminearum polypeptide SmAFP. The invention discloses an anti-fusarium graminearum polypeptide SmAFP of Sitodiplosis mosellana. The amino acid sequence of the anti-fusarium graminearum polypeptide SmAFP is shown as SEQ ID NO: 2. The invention also provides an application of the Sitodiplosis mosellana anti-fusarium graminearum polypeptide SmAFP. The Sitodiplosis mosellana anti-fusarium graminearum polypeptide SmAFP is used for resisting wheat fungus fusarium graminearum, namely, the Sitodiplosis mosellana anti-fusarium graminearum polypeptide SmAFP can inhibit the growth of fusarium graminearum.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating idesia anthracnose, fermentation liquor, a biocontrol bacterium agent and application. According to the invention, a biocontrol bacterium is separated and screened from microbial resources on healthy idesia leaves, is classified and named as Bacillus velezensis, is preserved in China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided by the invention has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, botrytis cinerea, fusarium oxysporum, alternaria alternata and pestalotiopsis which can cause plant diseases, and also has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, fusarium oxysporum, alternaria alternata and pestalotiopsis. The biocontrol bacterium, the fermentation broth and the biocontrol inoculant provided by the invention have potential application prospects in prevention and treatment of idesia anthracnose.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention discloses bacillus velezensis B127 and application thereof, the bacillus velezensis is preserved in China Center for Type Microbiological Culture Collection, the preservation number is CCTCC NO: M 20252878, the preservation date is December 15, 2025, the classification name is bacillus velezensis B127, and the preservation address is Wuhan University, Hubei Province. The bacillus velezensis B127 has a remarkable inhibition effect on various tobacco fusarium pathogenic bacteria, has good phosphorus and potassium solubilizing capacity, generates hydrolase related to inhibition of pathogenic bacteria and generates siderophore and indoleacetic acid for promoting plant growth, is a biocontrol bacterium with excellent comprehensive performance, and can be widely applied to the field of biological control. The method has good development and application prospects in the fields of agricultural disease control, soil improvement, microbial fertilizers and the like.

The invention discloses a preparation method and application of a functional nano-pesticide based on a random combinatorial library of polyhexamethylene biguanide hydrochloride analogues and phenolic hydroxyl group-containing natural small molecules. A PHMB analogue containing a mono-guanidyl or biguanidyl structure and a natural small molecule compound with 1-3 phenolic hydroxyl structures are selected, nano-particles with good dispersion coefficients can be formed in a sodium hydroxide aqueous solution with the pH value of 9-11 without additional carriers or stabilizers, and the limitation that traditional nano-pesticides depend on macromolecular carriers is broken through. The obtained nano-particles can be enriched on the surface of a plant pathogenic fungus cell membrane by utilizing the electrostatic adsorption effect of guanidyl, and the delivery effect of natural small molecules is enhanced, so that the antifungal activity of the nano-particles is improved. An antibacterial experiment result shows that part of the composition has a synergistic antibacterial effect on fusarium graminearum, and the diameter of an inhibition zone is superior to that of a single component. The method is high in construction efficiency, has good expansibility, can form a nano pesticide preparation library, and is suitable for plant disease prevention and control and environment-friendly material development.

The invention discloses application of an Fpg13 gene as a target in prevention and treatment of wheat diseases caused by fusarium pseudograminearum, and belongs to the technical field of biology. The invention researches and verifies that the Fpg13 protein is a fusarium pseudograminearum key toxic effect protein for the first time, and plays an important role in the process of infecting wheat by the fusarium pseudograminearum. By means of a PEG-mediated homologous recombination knockout technology, the effect protein Fpg13 gene of the fusarium pseudograminearum is knocked out, and the pathogenic ability of the fusarium pseudograminearum to wheat is weakened by the knockout mutant after knockout. The invention lays a theoretical foundation for developing the fusarium pseudograminearum small-molecule inhibitor by utilizing pathogenic factors of pathogenic bacteria, and has important significance for developing novel drugs of the pathogenic bacteria and inhibiting the generation of drug resistance.

The invention discloses a marine fungus fusarium keratinoides and application thereof. The fusarium keratinoides capable of efficiently producing the perindoprine is screened from marine sediments, and the content of the produced perindoprine can reach 561.7 mu g / L after the fusarium keratinoides is fermented in a GPY liquid culture medium, so that possibility and a foundation are provided for breeding strains suitable for industrial large-scale production of the perindoprine in the future; mass spectrum, hydrogen spectrum and carbon spectrum data of the separated perindoprine are completely consistent with perindoprine data reported in literatures.

Methods and compositions for identifying, selecting and / or producing a pathogen resistant plant, plant cell or seed (e. g,, a maize plant, plant cell or seed) are provided. Polynucleotides and polypeptides are provided, which when expressed in a plant increase disease and / or pathogen resistance (e.g., to Fusarium stalk rot) of the plant. Plants, plant cells and seed are provided comprising such polypeptides and polynucleotides, as are methods for their use.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention provides a rhizoma nardostachyos endophytic fungus strain and application thereof, the rhizoma nardostachyos endophytic fungus strain is derived from an endophytic fungus of a medicinal plant rhizoma nardostachyos, is separated from the medicinal plant rhizoma nardostachyos by adopting an endophytic fungus separation and purification technology, and is named Fusarium sp.GSJ1 through microbial taxonomic identification, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2025, and the preservation number is CGMCC NO.41765. The invention further discloses a preparation method of the strain. The fungus strain can be used for producing the wollaman penicillin through solid-state fermentation of rice, provides a new way for the source of the wollaman penicillin, and has important production practice value.

The invention discloses paenibacillus alvei NNUB1, a fungicide and application of the paenibacillus alvei NNUB1. The paenibacillus alvei NNUB1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.28005. The invention further discloses a preparation method of the paenibacillus alvei NNUB1. The newly separated paenibacillus alvei NNUB1 has excellent activity of resisting soil transmission plant pathogens, not only can antagonize various fusarium fungi, but also can effectively inhibit lauraceae lauraceae; the strain also has the characteristic of promoting plant growth, and the biomass of plants can be obviously improved; test data show that the strain can effectively inhibit soil-borne pathogens of continuously-cultivated soil, remarkably promote crop growth and has an excellent application prospect.

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.

The invention discloses mortierella alpina GRB-1, a fungicide and application of the fungicide, and belongs to the technical field of agricultural microorganisms. The Mortierella alpina GRB-1 is separated from root systems of healthy fruit trees in a continuous cropping apple orchard for the first time, and the Mortierella alpina GRB-1 has the capabilities of producing protease, producing siderophores, dissolving phosphorus, producing ammonia gas and producing auxin, can inhibit fusarium and has the application potential of relieving continuous cropping obstacles. The product prepared by combining the straw with crop straws has an excellent effect on relieving apple continuous cropping obstacles, can promote the growth of overground parts and underground parts of continuous cropping apple trees, improves the relative abundance of bacterial communities in apple continuous cropping soil and the nutrient content in the apple continuous cropping soil, improves the root activity of apples, and improves the survival rate of the apples. The method can be used for preventing and treating apple successive cropping obstacles.

The invention relates to the technical field of biology, and discloses an FPSE09041 protein and an application of a coding gene of the FPSE09041 protein in reducing pathogenicity of fusarium pseudograminearum. The amino acid sequence of the FPSE09041 protein is as shown in SEQ ID NO. 2, and the coding gene of the FPSE09041 protein is as shown in SEQ ID NO. 1. By knocking out the FPSE09041 gene, the expression of the FPSE09041 protein is inhibited, and the pathogenicity of the fusarium pseudograminearum to host plants is reduced. The research finds that the FPSE09041 is an indispensable key factor for pathogenicity of the fusarium pseudograminearum, and the survival and infection capabilities of pathogenic bacteria can be obviously weakened due to function deficiency of the FPSE09041. Therefore, the FPSE09041 protein and the coding gene thereof can be used as ideal molecular targets for developing novel, efficient and high-specificity antifungal agents, and a new technical path is provided for green prevention and control of wheat stem rot.

The invention discloses a Trichoderma reesei strain and an application of the Trichoderma reesei strain in degradation of mycotoxin and inhibition of toxin-producing bacteria. The Trichoderma reesei strain is a Trichoderma reesei strain GG-T40, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231843. The strain can synchronously and efficiently degrade eight kinds of mycotoxins such as AFB1, ZEN, T-2, ST, OTA, AOH, AME and TeA, and after the strain and the mycotoxins are co-cultured for 7 days, the removal rates of the eight kinds of toxins are all larger than or equal to 60%. The strain has strong bacteriostasis and toxin production inhibition effects, the toxin production inhibition rates of the strain to aspergillus flavus and fusarium graminearum are 62.5% and 74.4% respectively, and the strain can be applied to degradation of mycotoxin in agricultural products and food and inhibition of mildew and toxin production of the agricultural products and food.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof in plant disease control. A bacillus velezensis strain is separated and screened from rhizosphere soil of a wolfberry plant in a Nomuliflood farm in Qinghai province, the strain is bacillus velezensis G3 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 36136. The bacterial strain has a better antibacterial effect on pathogenic bacteria fusarium oxysporum of Chinese wolfberry root rot, also has an inhibiting effect on pathogenic bacteria of Chinese wolfberry verticillium wilt, botrytis cinerea, sclerotinia rot of colza, alternaria alternata, apple anthracnose, apple rot, pear wilt and wheat scab, and has broad-spectrum antibacterial activity; wide application prospects are realized in plant disease prevention and treatment.

The present invention relates to methods for controlling or preventing infestation of cereal plants by the phytopathogenic microorganism Fusarium pseudograminearum, comprising applying to a crop of plants, the locus thereof, or seed thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.