851 results about "Fusarium" patented technology

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.

The invention provides a salt-alkali-tolerant endophytic bacillus subtilis strain and application thereof in improvement of saline-alkali soil, and belongs to the technical field of biological bacteria. According to the invention, a salt and alkali tolerant endophytic bacillus strain NXO3 is obtained from stems and leaves of suaeda salsa, and is identified as bacillus subtilis (bacillus subtilis); the strain has the capabilities of dissolving phosphorus, fixing nitrogen and generating indoleacetic acid (IAA) and polyglutamic acid, and has a remarkable inhibition effect on a plurality of plant pathogenic bacteria such as fusarium neosporum, colletotrichum gloeosporioides, fusarium graminearum, fusarium oxysporum and cavity microsporum. The agricultural microbial agent containing 0.3 billion / g of NXO3 is prepared through formula optimization. The bacillus subtilis NXO3 has a remarkable saline-alkaline tolerance function, a plant growth promoting effect and a biocontrol effect on plant diseases, and the emergence rate and the yield of crops in saline-alkaline land can be increased.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention relates to the technical field of agricultural biological prevention and control, in particular to a pseudomonas chlororaphis subsp. Aurantiaca strain for preventing and controlling wheat basal stem rot and application of the pseudomonas chlororaphis subsp. Aurantiaca strain. The pseudomonas chlororaphis subsp. Aurantiaca strain for preventing and treating wheat stem rot is P. chlororaphis subsp. Aurantiaca, has very good antagonistic activity to fusarium pseudograminearum and fusarium flavum, the inhibition rate to the fusarium pseudograminearum strain reaches 76.07%, the inhibition rate to the fusarium flavum strain reaches 73.26%, and the inhibition rate to the fusarium flavum strain reaches 76.07%, so that the pseudomonas chlororaphis subsp. Aurantiaca strain for preventing and treating wheat stem rot can be used for preventing and treating wheat stem rot. The P. chlororaphis subsp. Aurantiaca can induce wheat to generate resistance to Fusarium pseudograminearum and Fusarium fulvum, the prevention and treatment effect on the Fusarium pseudograminearum strain reaches 55.77%, the prevention and treatment effect on the Fusarium fulvum strain reaches 51.19%, therefore, the P. chlororaphis subsp. Aurantiaca can significantly reduce the severity of the wheat basal stem rot, and the P. chlororaphis subsp. Aurantiaca can be used for preventing and treating the wheat basal stem rot. Meanwhile, the plant height, fresh weight and root length of wheat can be increased, and a remarkable growth promoting effect on wheat plants is achieved.

The invention discloses bacillus velezensis and application of the bacillus velezensis in prevention and treatment of basal stem rot, the classification name of the bacillus velezensis is bacillus velezensis GN-22, the bacillus velezensis is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the bacillus velezensis is CCTCC NO: M2025566, and the preservation date is March 24, 2025. The bacillus velezensis GN-22 disclosed by the invention belongs to rhizosphere growth-promoting bacteria, has a remarkable inhibition effect on different pathogenic fungi such as fusarium graminearum, fusarium pseudograminearum, fusarium asiaticum, fusarium verticillioides and fusarium oxysporum, and plays an important role in biological control of diseases such as corn and wheat stem rot; meanwhile, the strain has a growth promoting effect and can promote corn growth. The strain provided by the invention has the advantages of simple culture conditions, easiness in preservation, easiness in industrial production and good development and application prospects.

The invention relates to the technical field of microorganisms, and discloses trichoderma yunnanense and application thereof. The Trichoderma yunnanense CCTCC NO: M 2025645 provided by the invention is a dominant strain of a high-yield siderophore, the activity of the siderophore is 69.79%, and the Trichoderma yunnanense can be used for synthesizing and secreting carboxylic acid type siderophore; the strain has a sulfur oxidation function; in addition, the invention also provides an application of the trichoderma sp. In regulation and control of plant diseases, the trichoderma sp. Disclosed by the invention can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and the trichoderma sp. Can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae. The Trichoderma yunnanense provided by the invention is a strain separated from tobacco planting soil, and compared with a strain subjected to mutagenesis screening in an extreme environment and a laboratory, the Trichoderma yunnanense has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the Trichoderma yunnanense stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect.

The invention relates to fusarium venanii with high protein conversion rate and application of the fusarium venanii to improvement of hypha protein satiety, and belongs to the field of genetic engineering technology and food biotechnology. The fusarium venenatum FCPD disclosed by the invention is preserved in the China Center for Type Culture Collection on December 6, 2024, and the preservation number of the fusarium venenatum FCPD is CCTCC (China Center for Type Culture Collection) NO: M 20242752. The glucose consumption of a substrate for producing mycelia with the same dry weight by the fusarium venanii is reduced by 40-60% compared with that of a wild type, the protein conversion rate is improved by 70-80%, the grease content is improved by 90-110%, the gastric semi-emptying time of in-vitro simulated digestion is prolonged by 10-30%, and the quality of fusarium venanii mycelium protein is remarkably improved. Genetic engineering modification work can be further developed, and fusarium strains with high substrate conversion rate, high grease content and high satiety potential can be cultivated.

The invention relates to a cultivation nutrient medium and a preparation method thereof. According to the nutrient medium, peat is completely abandoned, the coco coir, the straw and the biochar are combined to reduce ecological burden, the nutrient medium is an environment-friendly medium, the biochar fixes carbon and reduces emission, and the soil structure can be improved after long-term use; vermiculite improves the water-holding capacity, perlite and straw form a stable pore structure, and the ventilation porosity reaches 25% or above. Wormcast and a microbial agent synergistically release nitrogen, phosphorus and potassium, and the fertilizer efficiency is lasting. The microbial agent can inhibit soil-borne diseases such as fusarium and reduce the death rate of cultivated seedlings. The biochar adsorbs salt and toxins, and the EC value is stabilized at 1.2-1.8 mS / cm. The cost of the selected raw materials is reduced by 35% compared with that of a traditional substrate, and the substrate is suitable for large-scale production, suitable for vegetable seedling culture, potted flowers, organic farms and soilless culture systems, and especially suitable for saline-alkali soil or continuous cropping obstacle areas.

The invention belongs to the technical field of modern agriculture, and particularly relates to a biocontrol bacillus amyloliquefaciens BA200 and application thereof. A new biological control bacterium BA200 is separated and identified, is named as bacillus amyloliquefaciens by taxonomy, and has a remarkable inhibition capability on two pathogenic bacteria, namely fusarium oxysporum and phytophthora vigna, causing the cowpea fusarium wilt, and the inhibition rate is up to 60% or above; fermentation liquor prepared from the bacillus amyloliquefaciens BA200 can remarkably reduce the morbidity and disease index of cowpea fusarium wilt, is a good biocontrol microorganism and has application and development potential and important significance in control of cowpea fusarium wilt, and the biocontrol bacterium bacillus amyloliquefaciens BA200 has a broad-spectrum biological control effect, can inhibit various phytopathogens and has a good application prospect. And valuable biocontrol bacterium resources are provided for preparation of biological bactericides.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention belongs to the technical field of microorganisms, and particularly relates to a P.adami FJ08 strain, a fungicide, a preparation method and application thereof, which can be used for inhibiting soil-borne diseases of radix pseudostellariae. The invention discloses a P.aryabhattai FJ08, the P.aryabhattai FJ08 is preserved in the Microbial Culture Collection Center of Guangdong Province, the preservation number is GDMCC No: 65907, and the preservation date is March 24, 2025. The P.aryabhattai FJ08 has the advantages that the P.aryabhattai FJ08 can be used for preparing the P.aryabhattai FJ08; the P.aryabhattai FJ08 is separated from the radix pseudostellariae seed ginseng, is safe and reliable, and has the capabilities of inhibiting the growth of fusarium oxysporum, producing IAA, producing ammonia, dissolving potassium and dissolving phosphorus, and the growth of pathogenic fusarium oxysporum can be effectively inhibited by soaking the radix pseudostellariae seed ginseng with the P.aryabhattai FJ08.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.

The invention relates to the field of gene engineering, in particular to a wheat antibacterial peptide and a gene and application thereof. The amino acid sequence of the wheat antibacterial peptide TaThiL1 is as shown in SEQ ID NO: 1. The wheat antibacterial peptide TaThiL1 is used for replacing chemical pesticides and preventing and treating plant fungal diseases, and the TaThiL1 protein has an inhibiting effect on the growth of fusarium graminearum and pseudofusarium graminearum.

The invention discloses a Brucella with antibacterial activity and application of the Brucella. The invention discloses Burkholderia sp.HNIV0701, and the preservation number of the Burkholderia sp.HNIV0701 is GDMCC No: 66007. The Burkholderia sp.HNIV0701 disclosed by the invention has a stable, efficient and broad-spectrum antibacterial property, has an inhibition effect on a plurality of plant pathogenic bacteria such as Botryosphaeria dothidea, Phytophthora nicotianae, Fusarium solani, Alternaria alternata, Thanatepora cucurbitae, Ralstonia solanacearum and the like, and also can be used for inhibiting Staphylococcus aureus and / or Listeria monocytogenes, so that the Burkholderia sp.HNIV0701 can be used for inhibiting a plurality of plant pathogenic bacteria such as the Botryosphaeria dothidea, the Phytophthora nicotianae, the Fusarium solanum, the Alternaria solanacearum and the like. The Brucella HNIV0701 can hydrolyze organic phosphorus and can be used for soil improvement. The Brucella HNIV0701 has nitrogen fixation capability and has the potential of promoting plant growth.

The invention belongs to the technical field of microbiology and agricultural application thereof, and particularly relates to bacillus aerosus, a microbial agent and application of the microbial agent. According to the invention, a strain with good antagonism on tobacco black shank and fusarium root rot is separated and screened from tomato roots, and is identified as aerobacillus, and chitinase, cellulase and protease are secreted to dissolve the cell wall structure of pathogenic bacteria hyphae and inhibit the growth of pathogenic bacteria, so that the pathogenic bacteria lose the infection ability, and the pathogenic bacteria are effectively prevented from being infected. The biocontrol microbial inoculant has the antibacterial effect on more than 10 plant pathogenic fungi, the antibacterial rate is greater than 80%, especially the antibacterial rate on phytophthora nicotianae and fusarium oxysporum is greater than 90%, and pot experiments and field experiments prove that the biocontrol microbial inoculant has the control effect on tobacco black shank and fusarium root rot obviously superior to those of commercially available biocontrol microbial inoculants and chemical agents; meanwhile, the tobacco seed germination can be promoted, the growth and development of field tobacco plants are promoted, the subculture stability is better, and the application prospect is wide.

The invention discloses a composite functional bacterial agent for preventing and controlling wheat scab and vomitoxin and application of the composite functional bacterial agent. The composite functional bacterial agent is applied to wheat scab and deoxynivalenol pollution caused by fusarium graminearum. The composite functional bacterial agent comprises a biocontrol strain bacillus subtilis DC-1 (Bacillus subtilis DC-1) and a degradation strain nocardia sp. MOZ-1 (Nocardia sp. MOZ-1), and the composite functional bacterial agent is prepared from the biocontrol strain bacillus subtilis DC-1, the degradation strain nocardia sp. MOZ-1 and the degradation strain nocardia sp. MOZ-1. The complex microbial inoculant can significantly inhibit infection of fusarium graminearum and reduce accumulation of DON in wheat, is low in production cost, reduces use of chemical pesticides and pollution of biotoxins in agricultural products, and can be effectively applied to the field of agricultural biological control.

The invention discloses a separation and purification method for improving protein purity and emulsifying property of fusarium venetii based on a cell wall simplification engineering strategy, and belongs to the technical field of food processing and protein separation and purification. The method for separating and purifying the Fusarium venanii protein comprises the following steps: dispersing Fusarium venanii fermented hyphae in water, and adjusting the pH value to 10-12 to obtain a Fusarium venanii solution; performing high-pressure homogenization, collecting supernate, adjusting the pH value to 4.4-4.5, performing centrifugation, and taking protein precipitate; redissolving the protein precipitate in water, adding chitinase for reaction, carrying out acid precipitation again after the reaction is finished, centrifuging, and taking the protein precipitate; and redissolving the protein precipitate in water, adjusting the pH value, dialyzing and drying to obtain the fusarium venanii protein. The fusarium protein obtained by the method is high in purity, has the characteristics of higher solubility, high interfacial activity, emulsifying property and the like, and is beneficial to food industrial application of the fusarium protein.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention belongs to the field of microorganisms, and particularly discloses a Pseudomonas klackmussii strain TYQ5 and application thereof, the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27214, and the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC). The applicant finds that the Pseudomonas kirschner can secrete auxin, can promote the growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum, and also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum. Furthermore, the pseudomonas kirschner has the capability of degrading vegetable straws and the capability of solubilizing phosphorus, so that the applicant considers that the pseudomonas kirschner has a remarkable application prospect in the fields of agriculture and environmental protection.

The invention belongs to the technical field of biological prevention and control, and particularly relates to a Burkholderia paragallinarum D15 and application of the Burkholderia paragallinarum D15. The Burkholderia parakholderia sp. D15 is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 25, 2024, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 1.61962. The Burkholderia parakholderia sp. D15 has the advantages that the Burkholderia parakholderia sp. According to the invention, a multifunctional strain Burkholderia paragallinarum D15 is separated from the rock wall of Danxia red rock, and the Burkholderia paragallinarum D15 has a relatively good antagonistic effect on most plant pathogens and has a relatively strong bacteriostatic effect on xanthomonas oryzae Xoo causing rice bacterial blight; the compound also has a relatively strong bacteriostatic effect on other pathogenic bacteria causing crop diseases, such as rice sheath blight disease, rice blast, fusarium graminearum, sclerotium rolfsii and ralstonia solanacearum, and lays a foundation for prevention and treatment of rice bacterial blight and development of related biological preparations.

The invention relates to the technical field of environmental microorganisms, in particular to arthrobacterium Arth.R8 and application thereof, and the preservation number of the arthrobacterium Arth.R8 is GDMCC No.63567. The arthrobacter Arth.R8 has the effects of fixing nitrogen, dissolving organic phosphorus, promoting growth and promoting nodulation. The arthrobacter Arth.R8 disclosed by the invention can be used for promoting absorption and utilization of nutrient substances such as nitrogen and phosphorus in a plant growth process, inhibiting growth of fusarium graminearum and promoting plant growth, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention relates to the technical field of biology, in particular to application of PnPUB35 protein and a coding gene thereof to regulation and control of plant wilt resistance. The invention provides a Ubiquitin ligase PnPUB35 protein containing a U-box structural domain E3 and a gene PnPUB35 of the Ubiquitin ligase PnPUB35 related to resistance to fusarium wilt of pepper, and after the pepper is inoculated with fusarium solani, the expression quantity of the Ubiquitin ligase PnPUB35 protein at the root of the pepper is increased. The resistance of a pepper PnPUB35 silent plant to fusarium solani is weakened, and the resistance of an arabidopsis thaliana PnPUB35 overexpression plant to fusarium solani is enhanced. The PnPUB35 gene disclosed by the invention plays a positive regulation role in pepper wilt resistance, and can be used as an important candidate gene for pepper wilt resistance breeding.

The invention relates to the technical field of genetic engineering, and discloses a fusarium pseudograminearum effect protein FpE01 and application thereof in wheat disease resistance. The amino acid sequence of the Fusarium pseudograminearum effect protein FpE01 is as shown in SEQ ID NO. 1. The nucleotide sequence of the coding gene of the Fusarium pseudograminearum effect protein FpE01 is as shown in SEQ ID NO. 2. The fusarium pseudograminearum effect protein FpE01 and the application thereof in wheat disease resistance are adopted, a knockout mutant of the coding gene FpE01 is constructed, the toxic function of the coding gene FpE01 in the process that wheat is infected by fusarium pseudograminearum is determined, then the effect protein is utilized to construct a wheat strain resistant to stem rot, and the wheat strain resistant to stem rot is obtained. And an excellent wheat material is provided for cultivation of basal stem rot resistant varieties.

The invention provides a fusarium specific gene segment and application of the fusarium specific gene segment in rapid detection of fusarium species. In particular, the present invention provides a fusarium specific segment. Furthermore, the invention also provides a primer pair for detecting fusarium species, and the primer pair is used for amplifying the fusarium specific segment disclosed by the invention. Furthermore, the invention also provides an application of the fusarium specific segment or the primer pair provided by the invention in detecting fusarium species. Through the fusarium specific section, different species in fusarium can be accurately distinguished, and the fusarium specific section can be applied to the field of plant quarantine to realize rapid and accurate detection of fusarium species.

Bacilli and combinations of Bacilli capable of germinating and growing under low temperature conditions; of associating with plant roots; of enhancing plant health, growth, and yield; and of protecting plants against phytopathogenic pests, such as Botrytis, Fusarium, Phytophthora and Pythium.

The invention relates to the technical field of environmental microorganisms, in particular to Chryseobacterium Chry.R1 and application of the Chryseobacterium Chry.R1, and the preservation number of the Chryseobacterium Chry.R1 is GDMCC (China General Microbiological Culture Collection Center) No.63568. The Chryseobacterium Chry.R1 has the effects of fixing nitrogen, inhibiting fusarium graminearum and promoting plant growth. The Chryseobacterium Chry.R1 disclosed by the invention can be used for promoting the absorption and utilization of nitrogen elements in a plant growth process, inhibiting the growth of fusarium graminearum and promoting the growth of plants, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention belongs to the technical field of agricultural biological control, and particularly relates to bacillus velezensis, a biological preparation and application of the bacillus velezensis in plant disease prevention and growth promotion. The bacillus velezensis is preserved in the China Center for Type Culture Collection, the preservation number of the bacillus velezensis is CCTCC NO: M 2025613, and the bacillus velezensis is classified and named as bacillus velezensis B54. Experiments show that the bacillus velezensis and the biological preparation thereof provided by the invention have broad-spectrum antagonistic activity, can effectively inhibit growth of pathogenic bacteria such as pythium spp and fusarium spp, induce plants to generate immune response, remarkably reduce the incidence rate of root rot and blight of the plants, can also generate plant growth stimulants, improve the development of plant root systems, improve the growth of the plant root systems and promote the growth of the plant root systems. The crop yield and quality are improved.

The invention discloses application of a TpGST gene in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. According to the application of the TpGST gene disclosed by the invention in regulating and controlling the ear rot and stem rot resistance of maize fusarium graminearum, the nucleotide sequence of the TpGST gene is shown as SEQ ID NO. 1. Fusarium graminearum is inoculated to ears and stems of an overexpressed TpGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic line is improved; fusarium graminearum is inoculated to ears and stems of the corn hybrid agricultural large 3138 overexpression TpGST transgenic line, and the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic hybrid is improved.