143 results about "Trichoderma reesei" patented technology

The invention provides recombinant escherichia coli with high yield of ergothioneine and a method for preparing ergothioneine, the recombinant escherichia coli co-expresses ergothioneine anabolic enzymes Egt1 and Egt2, the methyltransferase structural domain of the Egt1 is derived from trichoderma reesei, mycobacterium smegmatis EgtD or rhizopus nigricans, and the methyltransferase structural domain of the Egt2 is derived from the trichoderma reesei, the mycobacterium smegmatis EgtD or the rhizopus nigricans. The histidine sulfoxide synthase structural domain is derived from trichoderma reesei, schizosaccharomyces pombe or rhizopus nigricans, and the Egt2 is derived from claviceps purpurea, neurospora crassa or aspergillus niger. After the recombinant escherichia coli obtained by screening is cultured for 68 hours in a 5L fermentation tank, the yield of ergothioneine can reach 5g / L or above; wherein after recombinant escherichia coli co-expressing Egt1 enzyme (the structural domain of methyltransferase is derived from mycobacterium smegmatis EgtD, and the structural domain of histidine sulfoxide synthase is derived from trichoderma reesei) and Egt2 enzyme (derived from neurospora crassa) are cultured in a 5L fermentation tank for 68 hours, the yield of ergothioneine can reach 8.7 g / L, and the production intensity reaches 127.9 mg / (L.h).

The invention belongs to the technical field of agricultural microbial agents, and relates to an agricultural liquid microbial agent preparation method and a microbial agent, the method comprises the following steps: mixing bacillus amyloliquefaciens and trichoderma reesei, and carrying out primary aerobic fermentation; mixing a product subjected to primary aerobic fermentation with lactobacillus acidophilus subjected to expanding culture, and carrying out secondary micro-aerobic fermentation; and carrying out membrane separation treatment on the product subjected to secondary microaerobic fermentation, and concentrating the bacterial liquid. According to the microbial agent, through the synergistic effect of multiple strains, the inhibition rate of soil-borne pathogenic bacteria is increased, the occurrence rate of crop rhizosphere diseases is reduced, and then the yield and quality of crops are synergistically optimized. High-activity flora is reserved through gradient fermentation and membrane separation technologies, the continuous colonization capacity of thalli in deep soil is maintained in combination with biochar, a long-term stable field protection barrier can still be formed through flora interaction even if mechanical turning is not needed, and therefore disease prevention and control are achieved under the no-tillage or minimal-tillage condition.

The invention discloses a special organic fertilizer for soybeans and a preparation method thereof, and belongs to the technical field of organic fertilizers. The special organic fertilizer for soybeans is prepared from the following raw materials in parts by weight: 8-10 parts of fish protein modified charcoal, 40-50 parts of decomposed soybean meal, 20-25 parts of decomposed livestock and poultry manure, 15-20 parts of humic acid, 10-15 parts of plant ash, 3-5 parts of a seaweed extract and 0.1-0.3 part of ammonium molybdate. According to the organic fertilizer, the soybean meal fermented by the trichoderma reesei and the aspergillus awamori and the fish protein modified biochar are added, the two components are compounded with the cooked livestock and poultry manure, the humic acid, the plant ash, the seaweed extract and the ammonium molybdate to form the organic fertilizer, the nitrogen fixation capacity of the soybeans is effectively improved through the synergistic effect of various substances, and the growth and development of the soybeans are remarkably promoted; the soybean yield and quality are improved. In addition, the whole organic fertilizer preparation process is free of strong acid and strong alkali treatment, the environmental pollution risk is reduced, and the standard of organic agriculture is met.

The invention discloses a method for screening high-yield recombinant human lactoferrin through nucleic acid aptamer-assisted mutagenesis of a trichoderma reesei strain and application, and belongs to the technical field of microorganisms. The nucleic acid aptamer Lac-6a-FAM-BHQ1 capable of changing the fluorescence intensity according to the content of lactoferrin is constructed on the basis of the aptamer Lac-6a, and the nucleic acid aptamer can be used for rapidly selecting a mutant strain for producing high-titer recombinant human lactoferrin. The ARTP mutagenesis technology is combined with a nucleic acid aptamer auxiliary screening system, the mutant strain with the improved recombinant human lactoferrin yield is rapidly obtained from a large number of mutant strains, and the yield reaches 274.57 mg / L and is improved by 24.8% compared with that of an original strain. After the genes 111681 and 62716 are over-expressed in T. reesei, compared with a control strain, the yields of the recombinant human lactoferrin of the recombinant strain are respectively increased by 6.9% and 22.1%.

The invention relates to the technical field of resource utilization of traditional Chinese medicine wastes, in particular to a fermentation method of traditional Chinese medicine residues. The method comprises the following steps: ultrasonically crushing the traditional Chinese medicine residue to obtain a crushed material, carrying out microwave vacuum drying on the crushed material to obtain a dried material, carrying out low-temperature plasma sterilization on the dried material to obtain a sterilized material, mixing the sterilized material with trichoderma reesei, carrying out aerobic fermentation to obtain an aerobic fermented material, mixing the aerobic fermented material with yarrowia lipolytica, and carrying out anaerobic fermentation. By adopting the method provided by the invention, the flavone retention rate of the traditional Chinese medicine residues is increased, and meanwhile, the grease yield is also increased.

The present invention discloses a process for preparing lactic acid by mechanically enhanced ammonia method from straw-like biomass. The enzymatic hydrolysate of solid fibers obtained by the mechanically enhanced ammonia method from straw-like biomass is used as the carbon source of the fermentation medium in the process of fermenting lactic acid. The preparation of the carbon source in the fermentation medium comprises the following steps: S1: treating straw-like lignocellulosic biomass with a pretreatment liquid containing ammonia water and inorganic ammonium salts in a twin-screw device; S2: subjecting the obtained material to a heat preservation reaction, and after solid-liquid separation, obtaining solid fibers containing holocellulose; S3: enzymatically hydrolyzing the obtained solid fibers with Trichoderma reesei cellulase and xylanase, and performing solid-liquid separation, and the obtained supernatant is used as the carbon source in the fermentation medium for fermenting L-lactic acid. The present invention uses the sugar liquid obtained by enzymatic hydrolysis and saccharification of pretreated holocellulose, inoculates Bacillus coagulans to ferment L-lactic acid after supplementing nitrogen source and inorganic salts, and can greatly improve the concentration and conversion rate of L-lactic acid.

The invention discloses a Trichoderma reesei strain and an application of the Trichoderma reesei strain in degradation of mycotoxin and inhibition of toxin-producing bacteria. The Trichoderma reesei strain is a Trichoderma reesei strain GG-T40, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231843. The strain can synchronously and efficiently degrade eight kinds of mycotoxins such as AFB1, ZEN, T-2, ST, OTA, AOH, AME and TeA, and after the strain and the mycotoxins are co-cultured for 7 days, the removal rates of the eight kinds of toxins are all larger than or equal to 60%. The strain has strong bacteriostasis and toxin production inhibition effects, the toxin production inhibition rates of the strain to aspergillus flavus and fusarium graminearum are 62.5% and 74.4% respectively, and the strain can be applied to degradation of mycotoxin in agricultural products and food and inhibition of mildew and toxin production of the agricultural products and food.

The invention discloses a method for simultaneously producing ergothioneine and an enzyme preparation by utilizing trichoderma reesei, and belongs to the technical field of biological engineering. By optimizing the culture medium, a culture medium formula capable of enabling the trichoderma reesei which has almost no ergothioneine production capacity originally to synthesize the ergothioneine is screened out, and the trichoderma reesei can simultaneously produce the ergothioneine and an enzyme preparation. By further increasing the number of ergothioneine gene clusters of the trichoderma reesei, under the condition of not introducing any exogenous gene, an industrial production optimized strain of the ergothioneine and the enzyme preparation conforming to food safety is constructed, the yield of the ergothioneine and the enzyme preparation can be remarkably improved, and the production cost of the ergothioneine and the enzyme preparation is reduced. Meanwhile, the problems of biological safety, transgenosis safety and the like are solved, and technical support is provided for industrial production of ergothioneine and enzyme preparations.

The invention discloses a trichoderma reesei engineering bacterium for secreting and expressing egg white lysozyme and a construction method of the trichoderma reesei engineering bacterium, and belongs to the field of molecular biology and biotechnology. The invention firstly provides an egg white lysozyme mutant, and the amino acid sequence of the egg white lysozyme mutant is shown as any one of SEQ ID NO.2-4. The construction method of the trichoderma reesei engineering bacterium for secreting and expressing the egg white lysozyme comprises the following steps: by taking trichoderma reesei as a starting strain, transforming an expression module into the trichoderma reesei to construct the trichoderma reesei engineering bacterium; the expression module comprises a promoter, a secretory peptide gene, an egg white lysozyme gene and a terminator which are connected in sequence. The coding gene of the egg white lysozyme is transformed into a trichoderma reesei genome, so that a trichoderma reesei engineering strain for secreting and expressing the egg white lysozyme is successfully constructed. Meanwhile, different egg white lysozyme variants can bring different effects, and when the trichoderma reesei RUT-C30 is used as an original strain, the effect is superior to that of QM6a.

The invention discloses a construction method for improving gene editing efficiency of a recombinant trichoderma reesei engineering strain, and belongs to the technical field of gene editing. In the research, the T.reesei CJ2095 is taken as an original strain, the gene ku80 is knocked out, and the gene editing efficiency is improved to 7.14% from 1.04% of that of a control strain. In order to realize the recycling of the screening marker of the orotidine-5 '-phosphate decarboxylase gene pyr4, the gene pyr4 is knocked out. In addition, homologous recombinase rad51 and rad52 genes of a T.reesei endogenesis are respectively overexpressed, and the overexpressed gene rad52, namely the homologous recombination efficiency of the engineering strain T.reesei CJ2095 delta ku80 delta pyr4: rad52, can be improved to 43.75%, which is 42 times of that of an original strain.

In the present application, by knocking out the BG gene in Trichoderma reesei PX3 (a strain of cellulase transcription activator that can be activated continuously), the BG activity can be reduced under the condition of not reducing or even increasing the filter paper enzyme activity of the enzyme system, and a low BG enzyme activity and high filter paper enzyme activity strain is constructed. This strategy can avoid the negative impact on cellulase production after BG knockout, and the obtained strain can be used for efficient production of cellobiose or as a chassis strain for producing cellulase.

The invention belongs to the field of fungus secondary metabolites and application thereof, and particularly relates to trichoderma reesei HK1-12 as well as a secondary metabolite and application thereof, the collection unit is China General Microbiological Culture Collection Center (CGMCC), and the collection number is CGMCC No. 41951. The trichoderma HK1-12 has excellent characteristics in the aspects of growth promotion, plant protection and the like, and is expected to replace the existing similar products to be popularized and applied.

The invention discloses an efficient composting and resource utilization method for garden waste. The method comprises the following steps: S1, classification pretreatment: classifying the garden waste into high lignin, medium lignin and low lignin; respectively crushing until the particle size is less than or equal to 5mm (class A) or less than or equal to 3mm; adjusting the initial carbon-nitrogen ratio (C / N) of the mixed material to (35-45): 1 and the initial water content to 55-65%; s2, preparation and inoculation of a complex microbial inoculant: preparing a complex functional microbial inoculant containing a lignin degrading microbial inoculant, a cellulose / hemicellulose degrading microbial inoculant, a function enhancing microbial inoculant and a protective carrier, and inoculating the complex functional microbial inoculant into the pretreated material with the inoculation amount being 0.5-1.0% of the total dry weight of the material. The garden waste is finely classified and crushed according to the lignin content, and the physical structure and the initial C / N of the material are optimized; the white rot fungi, the trichoderma reesei, the bacillus subtilis and other strains in the complex microbial inoculant have a synergistic effect, and particularly, the white rot fungi specifically degrade lignin, so that the degradation bottleneck of a lignocellulose composite structure is effectively broken.

The present invention discloses a moisturizing and repairing bath oil, belonging to the technical field of daily chemical products. Its raw materials include PEG-5 brassinolide, lanolin oil, betula oil, michelia alba oil, macadamia nut oil, babassu oil, vanilla planifolia oil, a mixed fermentation extract of borage seeds and coix seeds, wherein the mixed fermentation extract of borage seeds and coix seeds is fermented with borage seeds and coix seeds as raw materials and trichoderma reesei as the fermentation strain. The bath oil provided by the present invention has remarkable effects of cleaning, moisturizing, repairing, antioxidant and the like.

The invention relates to a method for splitting, assembling and site-specific integration of large-fragment exogenous DNA (deoxyribonucleic acid) in Trichoderma reesei. Comprising the following steps: (1) constructing an arg:: Ptcu-Cas9-TtrpC-hph strain containing a Cas9 protein; (2) constructing a pUC19 <-> integration site: a pyr4 plasmid; (3) obtaining a plurality of target gene segments containing integration site homologous arms and having overlapping regions at the tail ends of adjacent DNA segments; (4) obtaining a gRNA expression cassette of a targeted genome integration site; (5) arg conversion: the arg is converted into a Ptcu-Cas9-TtrpC-hph strain; and (6) screening and verifying. According to the method, the CRISPR / Cas9 system is combined with the TAR in the trichoderma reesei for the first time, the exogenous large-fragment DNA is split into a plurality of small fragments, then the plurality of small-fragment exogenous DNA are assembled in the trichoderma reesei and are subjected to fixed-point integration, one-step editing of the large-fragment exogenous DNA is realized, and compared with a traditional genetics method, the gene editing efficiency in the trichoderma reesei is greatly improved.

This invention discloses a gene editing method and application for *Trichoderma reesei* based on a ribonucleoprotein complex. It aims to overcome the problems of low editing efficiency, easy generation of heterozygotes, and risk of exogenous DNA integration in *Trichoderma reesei* using plasmid-based CRISPR / Cas9 systems. This invention combines a dual-site cleavage strategy guided by double sgRNAs with direct RNP delivery. The method involves: designing two guide RNAs spaced 50-700 bp apart for the target gene, pre-assembling them with Cas9 protein in vitro to form an active RNP complex; delivering this complex along with exogenous donor DNA to *Trichoderma reesei* protoplasts. The exogenous donor DNA contains a left homologous arm, an editing sequence, and a right homologous arm, wherein the left and right homologous arms are homologous to the genomic sequences outside the dual cleavage sites on the target gene determined by the double sgRNAs, respectively; upon entering the cell, the RNP complex introduces dual-site cleavage at the target site, achieving precise editing under the guidance of the exogenous donor DNA.

The invention discloses a method for preparing a fermented feed from pomace and crop straw, and relates to the technical field of feed processing, and the method comprises the following steps: raw material treatment: performing rubbing treatment on corn straw; performing crushing treatment on the apple pomace; aerobic fermentation: uniformly mixing the filamentous material with the pomace powder, spraying a trichoderma reesei liquid into the mixture, and fermenting for 3-5 days under an aerobic condition; pressing and forming: after the aerobic fermentation is completed, pressing the mixed material into a cake belt with the thickness of 4-6mm through a double-roller tablet press; anaerobic fermentation is conducted, specifically, composite bacteria liquid is sprayed to the upper side and the lower side of the cake belt, the spraying amount of each square meter of the cake belt is 100-150 mL, and the composite bacteria liquid is composed of lactobacillus plantarum and saccharomyces cerevisiae according to the ratio of 3: 1; stacking the cake belt layers in a staggered manner; and feeding the cake belt into fermentation equipment, and fermenting for 2-3 days under an anaerobic condition. According to the method for preparing the fermented feed from the pomace and the crop straw, the feed is more balanced in nutrition, easy to digest and absorb and convenient for continuous fermentation production, and the fermentation product is convenient to store and transport.

The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof. Rut‑C30 The application discloses a recombinant Trichoderma reesei strain TriY120601 and a construction method and application thereof.

The present application provides a kind of shrimp shell biomass charcoal prepared by composite preparation and its preparation method and application, the present application is first prepared and obtained the composite preparation containing chitinase, bacillus licheniformis and bacillus subtilis, the chitinase is the chitinase derived from hartz wood enzyme is mutated, constructs mutant library, with trichoderma reesei as expression host, by screening, the excellent mutant Chi M1 obtained, its chitinase activity is significantly improved, is 2 times of original gene. By spraying the fermentation broth of the recombinant bacteria of the present application to obtain enzyme spray powder and the compounding of bacillus licheniformis powder and bacillus subtilis powder, the degradation efficiency of procambarus clarkia shrimp shell can be effectively improved. The composite preparation containing ChiM1 enzyme spray powder has good application prospect in the fields of aquaculture, feed additive and biomass resource utilization.

The application discloses a preparation method of small molecule peptides based on synergistic degradation of chicken eggshell membranes by molds and the small molecule peptides, and relates to the field of small molecule peptide preparation. The method comprises the following steps: mixing pretreated chicken eggshell membranes with mold fermentation liquor, and performing synergistic degradation; inactivating and centrifuging the degraded mixture, and collecting supernatant; purifying small molecule peptides in the supernatant through membrane separation technology; drying the purified small molecule peptide solution through a low-temperature spray drying method to obtain small molecule peptide powder; and the molds include Trichoderma reesei and Aspergillus niger. Through synergistic degradation of the two molds, the conversion of the chicken eggshell membranes is more thorough, the yield of the small molecule peptides is significantly improved, the total nitrogen recovery rate can reach 85.2%, and the product molecular weight distribution is concentrated.

The present application belongs to the technical field of nutritional functional food and health food, and particularly relates to a preparation method of marine fish skin collagen oligopeptide beneficial to promoting skin health, and a product and application thereof. The method comprises the following steps: after deep sea fish skin is pretreated by salt dissolution with a NaCl solution, trisodium citrate is added as an enzymolysis aid, and neutral protease (from Trichoderma reesei) and papain are sequentially used for two-step enzymolysis, and after enzyme inactivation, impurities are removed, sterilization is performed, and concentration, drying are performed to obtain the product. In vitro cell experiment verification shows that the product can significantly promote human skin fibroblast proliferation; in vivo animal experiment verification shows that the product can significantly increase skin moisture content, reduce transdermal water loss, increase hydroxyproline content, improve SOD activity and reduce MDA content. The product is suitable for the field of nutritional functional food and health food, and has a good application prospect in promoting skin health.

The invention discloses a method for preparing an amino acid fertilizer by multi-strain synergistic starter propagation and a liquid enzymolysis method, which comprises the following steps: selecting 25%-35% of bacillus subtilis, 10%-20% of trichoderma reesei, 15%-25% of aspergillus oryzae, 10%-20% of lactic acid bacteria, 5%-15% of saccharomyces cerevisiae and 5%-15% of aspergillus niger as composite strains; according to the method, six strains such as bacillus subtilis and trichoderma reesei form a composite microbial system, soybean meal and bran are used as raw materials, and the problems of low degradation efficiency, unstable enzyme activity and the like of a traditional process are solved through a process of'three-stage temperature-controlled starter propagation and four-stage liquid fermentation '; the method is characterized in that a'bacillus-mould-saccharomycetes-lactic acid bacteria 'four-flora synergistic system is constructed, a starter propagation-fermentation multi-parameter coupling regulation and control process is initiated, a'process-quality-effect' closed-loop mechanism is established, compared with a traditional process, the efficiency is improved by 35%-50%, the ton cost is reduced by 15%-20%, and the product is suitable for seed treatment and foliage spraying of various crops and has wide application prospects. The industrialization potential is remarkable, and the requirements of workers are met.

The invention belongs to the technical field of organic fertilizers, and particularly relates to a straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement and a preparation method of the straw-based biochar composite slow-release fertilizer. The preparation method comprises the following steps: (1) uniformly mixing sheep manure and pigeon manure, mixing the mixed manure with crushed corn straw to obtain a total material, inoculating bacillus subtilis, and composting and fermenting to obtain a primary organic matter; (2) fermenting the primary organic matter by using aspergillus niger, trichoderma reesei and saccharomyces cerevisiae to obtain a submerged fermentation organic matter; (3) crushing the submerged fermentation organic matter to prepare particles; the composite coating powder is scattered into the submerged fermentation organic matter, coating is conducted, and the coated organic matter is obtained; and (4) uniformly mixing the coated fermented organic matter, biochar, desulfurized gypsum, humus soil, a water-retaining agent and ammonium dihydrogen phosphate to obtain the straw-based biochar composite slow-release fertilizer for saline-alkali soil improvement. The organic fertilizer can improve the pH value of saline-alkali soil, reduce the salt content and increase the organic matter content.

The invention discloses a Trichoderma reesei engineering bacterium for secreting and expressing plastic degrading enzyme as well as a construction method and application of the Trichoderma reesei engineering bacterium, and belongs to the technical field of gene engineering. An expression plasmid of the plastic degrading enzyme is constructed, trichoderma reesei is converted, and the trichoderma reesei engineering bacterium for secreting and expressing the plastic degrading enzyme is constructed. In the Trichoderma reesei engineering bacteria, an endogenous protein coding gene cbh1 with the highest secretory expression quantity in Trichoderma reesei is replaced with a gene for coding the plastic degrading enzyme. The Trichoderma reesei engineering strain for secreting and expressing the plastic degrading enzyme is successfully constructed for the first time, and the Trichoderma reesei engineering strain constructed by the invention can significantly increase the productivity of the plastic degrading enzyme, and has potential to support future large-scale application.

The invention provides a preparation method of anti-inflammatory soothing and repairing microspheres based on Trichoderma reesei-Lactobacillus johnsonii two-stage fermentation. According to the preparation method, Trichoderma reesei fermentation products, Lactobacillus johnsonii metabolites, specific plant active ingredients and mussel mucoprotein are included. According to the invention, the mussel mucoprotein-trichoderma reesei-lactobacillus johnsonii ternary synergistic anti-inflammatory antibacterial system microspheres are innovatively constructed, the targeted delivery efficiency of active ingredients is remarkably improved through the adhesion characteristic of mussel mucoprotein, and the technical bottlenecks of poor permeability and short action time of a traditional anti-inflammatory preparation are solved. A multi-strain synergistic fermentation process is adopted, beta-glucan secreted by trichoderma reesei and short-chain fatty acid generated by lactobacillus johnsonii generate a synergistic anti-inflammatory effect, and meanwhile, an enzymolysis technology is combined to realize efficient conversion of plant active ingredients, so that the inhibition effect on key inflammatory factors such as TNF-alpha and IL-6 is remarkably enhanced. According to the system, inflammatory pathways such as NF-kappa B and the like are regulated and controlled through multiple targets, the inflammatory reaction is reduced while the growth of pathogenic bacteria is inhibited, a remarkable broad-spectrum antibacterial and anti-inflammatory synergistic effect is shown, and an innovative solution is provided for developing an efficient and safe anti-inflammatory antibacterial preparation.

The present application relates to the technical field of microbial degradation of rhizoma polygoni multifidi residues, and discloses a composite microbial agent and a preparation method and application thereof.The composite microbial agent contains trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis; wherein the proportion of the spores or spores of the trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis in the composite microbial agent is 1-6:1-6:1-6:1-6:1-6:1-6:1-6.Based on this, the composite microbial agent has good degradation efficiency on rhizoma polygoni multifidi residues, can accelerate the composting process of rhizoma polygoni multifidi residues, shorten the composting period of rhizoma polygoni multifidi residues, and the composting product after composting is stable in quality and is a high-quality organic fertilizer, so that effective utilization of resources is realized.

The invention provides an efficient microbial fermentation inoculant for straws and a preparation method thereof, and relates to the technical field of biodegradation. The efficient microbial fermentation inoculant for the straw is formed by compounding four strains including trichoderma reesei, streptomycete, lactobacillus plantarum and bacillus amyloliquefaciens according to the mass ratio, the ratio of the viable count of the four strains is that the ratio of the viable count of the trichoderma reesei to the viable count of the streptomycete to the viable count of the lactobacillus plantarum to the viable count of the bacillus amyloliquefaciens is 3: 2: 2: 1.5, the total viable count of the inoculant is larger than or equal to 1.3 * 10 CFU / g, and the cellulase activity is larger than or equal to 85 U / g. The laccase activity is greater than or equal to 42U / g. The invention can effectively solve the industrial pain points of difficult degradation of straws, poor quality and weak stability, and in agricultural production, the microbial inoculum can increase the content of organic matters in soil, can reduce the feed cost in the feed field, and can be used for production of straw-based organic fertilizers in the environmental protection field, and can reduce the use amount of chemical fertilizers.

The present invention relates to the field of genetic engineering technology, and specifically provides a high-yield xylanase-producing Trichoderma reesei strain and its application. The applicant firstly isolates the strain from Penicillium decumbentum ( Penicillium reclining ) was overexpressed in a Trichoderma reesei host to construct a recombinant expression strain. This strain was then used as a starting strain for UV mutagenesis, resulting in a mutant strain that significantly increased xylanase expression. The mutant strain is deposited with CCTCC NO: M2021920. This mutant strain can be widely used in xylanase production, helping to reduce the production cost of the enzyme.

The invention belongs to the technical field of biology, and particularly relates to a trichoderma reesei expression site H2 and application thereof as well as a recombinant trichoderma reesei strain for producing thaumatin II protein as well as a construction method and application of the recombinant trichoderma reesei strain. The Trichoderma reesei expression site H2 provided by the invention is located at the 210th site in a gene segment as shown in SEQ ID NO.6 in a Trichoderma reesei genome, and an expression level equivalent to that of a cbh1 site can be obtained by inserting an exogenous gene into the site. The recombinant trichoderma reesei strain for producing the thaumatin II protein provided by the invention contains a gene for coding the thaumatin II protein, which has a nucleotide sequence shown as SEQ ID NO.1, and the gene has high translation efficiency in the trichoderma reesei, so that the recombinant trichoderma reesei strain can efficiently produce the thaumatin II protein, and the proportion of the thaumatin II protein in the obtained total protein is high; the method has important significance in reducing the production cost of the thaumatin II protein and promoting the application of the thaumatin II protein in the food industry.

The invention discloses a Trichoderma reesei engineering strain for producing trans-aconitic acid as well as a construction method and application of the Trichoderma reesei engineering strain, and relates to the technical field of biological engineering. The construction method comprises the following steps: transforming a coding gene of mitochondrial tricarboxylic acid transport protein and a coding gene of aconitic acid isomerase into a trichoderma reesei host bacterium, and carrying out gene recombination construction to obtain the trichoderma reesei engineering strain, the amino acid sequence of the mitochondrial tricarboxylic acid transporter is as shown in SEQ ID NO. 1; the amino acid sequence of the aconitic acid isomerase is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. A new production strain is provided for microbial fermentation production of trans-aconitic acid, a large amount of trans-aconitic acid can be produced through direct fermentation by using common carbon sources such as glucose or cellulose, and the highest yield can reach 26.5 g / L.