600 results about "Trichoderma reesei" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

The invention discloses microbial straw decomposition additive, which belongs to the field of straw decomposition additive and is obtained through combining four bacteria: bacillus subtilis, aspergillus niger, trichoderma reesei and saccharomyces cerevisiae. The microbial straw decomposition additive utilizes the temperature rise benefit of the aspergillus niger spore enzyme activity for raising the environment temperature of decomposition, the further temperature rise benefit in the growth process of the bacillus subtilis is cooperated, and the temperature required by the decomposition is ensured, so the decomposition effect of straws is improved. The microbial straw decomposition additive has the obvious temperature rise benefit and can be used for the in-site field returning decomposition of the straws, the straw decomposition can be better accelerated in low-temperature and normal-temperature environment, the bottleneck problem of straw utilization is solved, the straw burning can be effectively reduced, the decomposition work procedure and the labor cost are saved, the fertilizer feeding is reduced, the soil structure is improved, and the planting benefit is improved.

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

The invention discloses one kind of bread zymogens agents for plants straw especially fit to fermentation of dried and yellow straw contained plant lactobacillus, microzyme, trichoderma reesei, bacillus subtilis, which is preparation by mixing bacterial liquid in proportion after third class general cultivation in solid test tube, liquid triangle bottle and liquid malt wort for all bacterial, and state fermentation cultivation to grow up. All bacterial in this invention are cultivated separately, mixed till the stage of producing bacterial in liquid, which is more suitable to different cultivation conditions of different bacterial and enables every bacterium with the strongest activity.

The present invention provides an insertion, deletion and/or substitution mutein of wild-type Trichoderma reesei β-mannanase having enhanced thermostability, proteolytic stability, specific activity and/or stability at low pH, a nucleic acid molecule encoding said mannanase mutein, a composition comprising said mannanase mutein; a method for its preparation, and its use for food and feed processing, for coffee extraction and the processing of coffee waste, as a supplement to food and feed, for enzyme aided bleaching of paper pulps, as bleaching and/or desizing agent in textile industry, for oil and gas well stimulation by hydraulic fracturing, as detergent, for removal of biofilms and in delivery systems, or for the processing of renewable resources intended for the production of biological fuels.

The invention provides a method for producing an organic fertilizer from municipal dry branch/fallen leaf waste and application thereof. The method comprises the following steps: collecting branches, fallen leaves and other municipal greening wastes, and mixing Aspergillus niger, Saccharomyces cerevisiae, Bacillus subtilis and Trichoderma reesei to obtain a composite microbial inoculant; adding bran coat, chaff powder or peanut hull meal, the composite microbial inoculant, urea and potassium dihydrogen phosphate, thoroughly mixing, piling into a 1.5-2m-high cone, and controlling the water content at 60-70%; carrying out hide lifting on the pile in the composting process, and fermenting for 20-30 days; and carrying out aerobic and anaerobic fermentation on the dry branch/fallen leaf waste by using the microbial inoculant to obtain the organic fertilizer. The preparation technique is simple; and the prepared organic fertilizer can improve the soil and reduce the environmental pollution caused by the waste.

The invention provides the application of trichoderma reesei and sedum plumbizincicola in the remediation of cadmium-polluted farmland soil. The application comprises the following steps of: cutting sedum plumbizincicola seedlings with about 10cm of plant height into soil to be remedied, applying trichoderma reesei FS10-C fermentation liquor or microbial preparation to the rhizosphere of the plant, keeping the soil water content to be 70%wt of the maximum field capacity in the process of remediation, and scissoring the plant along the surface of soil after the plant grows for 60-150 days to complete one remediation process. According to the application, the plant remediation efficiency of the cadmium-polluted farmland soil can be improved; and the sedum plumbizincicola plant remediation efficiency can be improved according to the trichoderma preparation, the biomass of the soil microorganism can be enhanced, the micro-ecological environment of the soil can be improved, and the application is good in ecological efficiency, and suitable for the remediation of the cadmium-polluted farmland soil.

The invention discloses a bio-enzyme and microorganism containing compound feed additive and a preparation process thereof, which solve the problems of high production cost and less viable count of beneficial microorganisms in the prior art. The bio-enzyme and microorganism containing compound feed additive is characterized in that the additive is prepared by compounding a viable organism preparation (prepared by carrying out liquid fermentation and low-temperature drying on bacillus subtilis and brewer's yeast) with an enzyme preparation (prepared by carrying out solid fermentation and drying on trichoderma reesei and Aspergillus niger); and the viable count of beneficial microorganisms in each gram of the product is greater than 8*10<9> cfu/g, the cellulase activity (refer to the quantity of cellulose degraded by each gram of the product per minute) is greater than 500 mg, the alpha-amylase activity (refer to the quantity of starch hydrolyzed by each gram of the product per minute) is greater than 3000 mg, and the protease activity (refer to the mount of an amino acid generated by caseins hydrolyzed by each gram of the product per minute) is greater than 400 mg. The bio-enzyme and microorganism containing compound feed additive disclosed by the invention has the advantages that: the feed utilization rate of livestock can be improved in a short term, the intestinal digestive flora of livestock is improved and balanced, the breeding cost is reduced, and the feed conversion rate and the animal production performance are improved; and meanwhile, the additive is simple in preparation process, low in production cost, and suitable for industrialized production.

Disclosed are a fertilizer for advancing alkaline land and its preparing method, comprising of microbe compound bacterium solution and dry matter and their mess ratio is 1:80-150, the microbe compound bacterium solution is formed by plant lactobacillaceae, distillers yeast . The invention is made by completely using organic substance to fermen, which can adyance alkaline land as wellas be fertilizer for green organic agricultural products.

The invention discloses a straw-decomposing inoculant which comprises the following active components: bacillus subtilis, aspergillus niger, trichoderma viride, phanerochaete chrysosporimn and geobacillus stearothermophilus. In the straw-decomposing inoculant, the total content of effective viable bacteria is (1-2)* 10<8>/ml. With adoption of the straw-decomposing inoculant, the fertilizer prepared from straws can be rapidly degraded, and the normal compost fermentation is performed in advance for at least 15 days. When the straw organic fertilizer prepared from the straw-decomposing inoculant is used as the base fertilizer, alkali-hydrolyzale nitrogen, rapid available phosphorus, potassium and organic contents in the soil are higher than that in the soil in which straws are directly utilized and no fertilizer is applied, and the urease activity and cellulase activity in the soil are obviously higher than that in the soil in which straws are directly utilized and no fertilizer is applied.

The invention provides an antibiotic-free mixed feedstuff and a preparation method thereof. The invention belongs to the field of animal feedstuffs. According to the invention, a mixed feedstuff containing Chinese herbal medicines is mixed with a mixed fermentation material containing various probiotics according to a certain proportion, such that the feedstuff provided by the invention is obtained. The mixed feedstuff containing Chinese herbal medicines comprises half-dried ginkgo leaves or ginkgo leaf extraction residues, Japanese honeysuckle stems, balloon flower root, soybean meal, peanutmeal, corn powder, wheat flour, calcium hydrogen phosphate, common salt, composite microelement, mulvital, and selenium-enriched yeast. The mixed fermentation material containing various probiotics comprises an enterococcus faecalis fermentation material, a bacillus licheniformis fermentation material, a saacharomyces cereisiae fermentation material, a Trichoderma reesei fermentation material, and an aspergillus niger fermentation material, which are mixed according to a certain proportion. Microbe strains are processed through targeted acclimatization, and are cultivated by using a solid fermentation medium, such that the mixed fermentation material is obtained. The antibiotic-free mixed feedstuff provided by the invention assists in promoting growth, and brings no toxic or side-effect. The feedstuff causes no antibiotic residue, no pollution, and no drug resistance. With the mixed fermentation the utilization rate of the feedstuff can be improved, healthy growth of live stocks can be promoted, and meat qualities of the live stocks can be improved.

The present invention provides a method for preparing bean dregs soluble dietary fiber by zymotechnics. Firstly, to dry the fresh bean dregs at the temperature of 60 to 80 DEG C for 12 to 14 hours, then to comminute until passing 40 mesh sieve, and to confect into ferment culture medium according to the ratio of 1 : 20 to 25 of bean dregs : water, and regulating the pH value to 4 to 4.5, then to disinfect at the temperature of 121 DEG C for 15 to 20 min to obtain liquid; to inoculate leeb wood mildew into the culture medium according to the 3 to 4 percent of the liquid volume ratio, then to shake bed culture at the speed of 140 to 160 r/min for 3 to 4d at the temperature of 28 to 30 DEG C. After sterilization, filtering and concentrating, to obtain deposit by non-water ethanol deposition, then to dry the deposit in vacuum to produce finished product. The present invention condition is geniality, equipment is simple, enzyme specificity is high, and can improve activity component amount maximum, has no pollution to circumstance, the SDF content in the bean dregs is increased greatly.

The invention discloses a composite aroma-producing microorganism as well as a preparation method and application thereof. The composite aroma-producing microorganism is formed by compounding bacillus subtilis, trichoderma reesei, aspergillus niger and hansenula polymorpha at a wet thallus mass ratio of 5:2:1:1. The composite aroma-producing microorganism disclosed by the invention can be used for preparing a filter material for a cigarette filter and greatly improving the aroma and taste of the cigarettes, and has good application value in the cigarette processing production.

The invention relates to a process for preparation of fermentation and induction of ramie degumming compound enzyme, which includes the steps of (1) induction preparation of ramie degumming compound enzyme system of trichoderma reesei, (2) determination of the activity of cellulose, xylanase and pectinase and (3) the processing of ramie by crude enzyme liquid which is obtained by fermentation. The invention can obtain the higher enzyme activity, is better qualified to the compound enzyme system of the ramie degumming, and is low in costs, simple and convenient in operation and easy in industrial production.

The invention discloses a recombinant vector and recombinant bacterium of Trichoderma reesei beta-glucosaccharase gene BGL1, and expression of the Trichoderma reesei beta-glucosaccharase gene BGL1 in the recombinant bacterium. The recombinant vector containing the Trichoderma reesei beta-glucosaccharase gene BGL1 is constructed by the following steps: (1) carrying out PCR (Polymerase Chain Reaction) amplification on the Trichoderma reesei beta-glucosaccharase gene BGL1, and connecting to a TA vector; and (2) constructing the Trichoderma reesei beta-glucosaccharase gene to a pichia pastoris expression vector. The recombinant bacterium disclosed by the invention can effectively express the target protein Trichoderma reesei beta-glucosaccharase BGL1. The invention overcomes the defect that a great deal of beta-glucosaccharase can not be purified from Trichoderma reesei in the prior art. Lots of waste lignocellulose exists in the natural world. The method provided by the invention can increase the yield of beta-glucosaccharase, and has very important meanings in enhancing degradation efficiency of cellulose, efficiently producing energy from waste cellulose and solving the problem of energy crisis at present.

The invention provides an engineering strain of recombinant expression lipase. Lipase gene obtained by cloning in Aspergillus niger is transferred into Trichoderma reesei, so that Trichoderma reesei engineering strain can be constructed and has the preservation number of China Center for Type Culture Collection (CCTCC) No.: M2012483. The recombinant expression lipase has the optimal action pH of 5.0 and the optimal action temperature of 30 DEG C, and the tolerance of the recombinant expression lipase to gastric juice, pepsase and artificial intestinal juice can be enhanced. The lipase remarkably improves the utilization rate of oil matter in feed, thus remarkably reducing the addition of oil in the feed and reducing the cost of the feed.

The invention provides a trichoderma reesei mutant strain VLKDN-1. The preservation number of the stain VLKDN-1 is CCTCC NO:M 2014506. A trichoderma reesei mutant strain capable of producing cellulase in high yield is obtained by adopting an ultraviolet-induced mutation technology combined with shake flask selection. Compared with a starting strain, the enzyme activity of the fermentation supernatant of mutant strain trichoderma reesei VLKDN-1 is increased by 235%, the content of protein is increased by 157%, the colonial morphology of the mutant strain is obviously smaller than that of the starting strain; the hyphae are shorter than that of the starting strain; the branches are more than that of the starting strain. The viscosity of a fermentation strain solution is remarkably reduced by virtue of the characteristics of short hyphae and more branches of the small colonial morphology of the mutant strain in the production process; the purposes of reducing the stirring rate and improving the dissolved oxygen are achieved; the production cost is reduced; the trichoderma reesei mutant strain VLKDN-1 is wide in application prospect.

The invention discloses a composite microbial fertilizer bactericide for disease prevention and growth promotion of tobacco and an application thereof. The bactericide can be used for promoting tobacco growth and/ or preventing tobacco disease, and comprises active components namely Trichoderma ressei ACCC 30150 and Streptomyces silaceus ACCC 40021. The bactericide is environment-friendly, can inhibit dug resistance development of a disease pathogen, is favor of popularization of green food and organic agriculture, is low in cost and non-pollution, and safe to tobacco crops. By introducing antagonistic microbe in soil, through biological control, fungus and bacterial diseases can be effectively prevented, environment is effectively improved, agroecological system balance is maintained, and a foundation for sustainable development of tobacco can be laid.

The invention discloses aroma-enhancing puffed tobacco stem particles and a preparation method and application thereof. The preparation method of the aroma-enhancing puffed tobacco stem particles comprises the steps that a micropore structure is formed in each tobacco stem, and the tobacco stems are dried and smashed after being fixed and formed to form particles; the tobacco stem particles are inoculated with a compound aroma-generating microorganism, fermental cultivation and drying are carried out on the tobacco stem particles and the compound aroma-generating microorganism, and then the aroma-enhancing puffed tobacco stem particles can be obtained. The compound aroma-generating microorganism is formed by compounding bacillus subtilis, Trichoderma reesei, Aspergillus niger and Hansenula polymorpha according to the wet-cell mass ratio of 5:2:1:1. According to the aroma-enhancing puffed tobacco stem particles and the preparation method and application thereof, the tobacco stems serve as raw materials to be processed to obtain the puffed tobacco stem particles, then the compound aroma-generating microorganism is added, and serving as a filter material of a filter tip, the finally-obtained aroma-enhancing puffed tobacco stem particles have the advantages of being high in filter efficiency, strong in adsorption capacity, free of offensive odors and consistent with tobacco in aroma and have good application prospect.

The invention provides a method for improving a submerged fermentation level of trichoderma reesei cellulase liquid. The method provided by the invention comprises the following steps of: first-grade seed culturing: preparing a spore suspension solution from activated trichoderma reesei CICC 13052 bacterial strain on a PDA (Potato-Dextrose-Agar) slant culture medium and inoculating the spore suspension solution into a fresh seed culture medium according to the inoculating amount of 3% to be cultured; and fermenting and culturing: inoculating the first-grade seeds in a logarithm growing periodinto a fresh fermenting culture medium to be cultured and controlling the pH values and dissolved oxygen in a pot of the fermenting process by sections to maintain the pH values and dissolved oxygen of the different periods at the different levels, and carrying out feed supplement and controlled culturing. By utilizing the method provided by the invention, the liquid is fermented for 168 hours; the enzyme activity of the fermenting liquid is measured by utilizing an international standard method which is recommended by the IUPAC (International Union of Pure and Applied Chemistry); and the enzyme activity of filter paper is 14.2 IU/mL and is improved by 67.1% compared with the interval fermenting enzyme activity level (8.5 IU/mL) before the process is optimized.

The invention discloses a preparation method of a straw decomposition agent. The straw decomposition agent is prepared by using bacillus subtilis, aspergillus oryzae, lactobacillus plantarum, trichoderma reesei and saccharomyces cerevisiae as preparation strains, carrying out activation and primary and secondary culture on the strains respectively to obtain various strain solutions with bacterial count of 106-108/ml and mixing the strain solutions according to a certain proportion. The straw decomposition agent has the beneficial effects that the straw decomposition agent can be used for quickly degrading straws to prepare fertilizers and ensures that the straw decomposition time is at least 15 days ahead of the normal composting fermentation time; organic straw fertilizers prepared by the preparation method are used as base fertilizers, so that the contents of available nitrogen, rapidly available phosphorus, potassium and organic matters in soil are higher than those of available nitrogen, rapidly available phosphorus, potassium and organic matters in the soil in which straws are directly used and fertilizers are not applied; the urease activity and cellulase activity of the soil are obviously improved compared with those of the soil in which straws are directly used and fertilizers are not applied.

Fungal glucoamylases from Aspergillus fumigatus - expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

The invention which relates to the field of genetic engineering provides a constitutive expression cassette of Trichoderma reesei. The expression cassette comprises a foreign target gene, a promoter of a pyruvate decarboxylase gene (Ppdc) of Trichoderma reesei, and a terminator of the pyruvate decarboxylase gene (Tpdc) of Trichoderma reesei, wherein the nucleotide sequence of the Ppdc is represented by SEQ ID NO.1, and the nucleotide sequence of the Tpdc is represented by SEQ ID NO.2. The expression cassette which comprises the sequence of the Ppdc of Trichoderma reesei and the sequence of the Tpdc of Trichoderma reesei can express genes from animals, plant or fungi without induction, and can properly modify expression products, protein expression can be carried out with the highly efficient synthesis and secretion capability of Trichoderma reesei, the protein expression level is high, and excessive foreign proteins in the expression products can be avoided. A recombinant strain of the present invention can highly express xylanase without cellulose, and the xylanase has an important application in the papermaking industry.