27results about How to "Increase vitality" patented technology

The present application provides a kind of prevention and control method of green moss in water lily planting pool, specifically belongs to water quality control technical field.The prevention and control method of green moss in water lily planting pool described in the present application includes the following steps: before planting water lily, water injection, water level is controlled at 15~20cm, copper sulfate is applied to kill green moss and its spores, keep water body not to flow during disinfection;After disinfection, apply sodium thiosulfate, and plant water lily;After planting water lily, keep replenishing water, control water level to 40~50cm, when green moss first outbreak, apply mineral fulvic acid potassium and composite bacillus;Subsequent green moss outbreak, apply mineral fulvic acid potassium, or apply mineral fulvic acid potassium and composite bacillus.The prevention and control method described in the present application is safe and efficient, which can quickly and effectively remove green moss without inhibiting the growth of water lily, and also does not affect the harvest of water lily.

The present application relates to the field of plant tissue culture technology, and particularly discloses a lily stem cell abundance ex vivo regulation method. The lily bulb is used as the starting material, stem cell magnitude amplification and regulation culture can produce lily tissue organ materials with high stem cell abundance. The present application provides sterile lily materials with high stem cell abundance, which has the characteristics of strong regenerative ability, high proliferation rate, etc., is the basis for protoplast preparation and high-efficiency regeneration, and is suitable for different lily varieties, and provides technical support for cell engineering breeding, seedling scale breeding, etc.

This invention discloses a method for producing crop cultivation substrate using sugarcane filter mud and sugarcane pith as main raw materials, comprising the following steps: (1) dissolving and diluting 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylic acid in water to obtain a solution; (2) mixing sugarcane slurry, sugarcane pith, the 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylic acid solution, fermentation agent, and improver, adjusting the moisture content, and then composting and fermenting. The compost is turned over every few days and continuously composted and fermented for several days to obtain the crop cultivation substrate. The crop cultivation substrate produced by this invention is of excellent quality, allowing direct planting of plants without root burn. Therefore, this invention, using sugarcane filter mud and sugarcane pith as main raw materials to prepare and produce crop cultivation substrate, can effectively solve the problem of comprehensive utilization of sugarcane filter mud and improve product sales.

This invention discloses a method for preparing and applying a sodium borohydride-modified self-supporting anode for AEM water electrolyzers. Firstly, an amorphous ternary metal hydroxyl oxide precursor is grown in situ on a nickel substrate via room temperature liquid phase deposition. The process involves the presence of Co in solution. 2+ Fe 3+ Ni ions are introduced through slight corrosion of the nickel foam matrix. 2+ The ions, the three, and the OH- produced by hydrolysis ⁻ Co-deposition occurs under the influence of dissolved oxygen, leading to the self-assembly of amorphous nanosheet structures on the substrate surface. Subsequently, a mild chemical reduction treatment with sodium borohydride solution is used to obtain a structurally stable and high-performance self-supporting electrode. The method described in this embodiment is mild, simple, low-cost, and has good versatility.

The application discloses a prenylated polyketide compound, a preparation method and application thereof, and the prenylated polyketide compound is separated from rice fermented with Xylaria nigriplicata. The neuroprotective effect of the prenylated polyketide compound on glutamate-induced excitotoxicity of PC12 cells is clarified through comprehensive evaluation of cell viability, apoptosis and key oxidative stress markers.

This invention provides an oyster peptide and its application in enhancing immunity. The oyster peptide has a novel amino acid structure. The oyster peptide can enhance the activity of immune cells, promote the proliferation of immune cells, promote the development and maturation of immune organs, regulate the in vivo immune environment, promote the secretion of immunoglobulins, and improve humoral immunity.

This invention relates to the field of broad bean paste technology and discloses a low-salt broad bean paste production process based on the synergistic fermentation of compound microorganisms. The process includes: uniformly mixing broad bean koji with drained chili embryos to obtain a solid mixture; spraying an A-phase initiation conditioning solution onto the solid mixture and inoculating it with *Lactobacillus plantarum*, mixing until moistened to obtain a primary fermentation mash; controlling the primary fermentation mash to undergo constant-temperature fermentation until the pH value drops to a preset acidic range to obtain an acidified mature mash; injecting a B-phase hypertonic regulating solution into the acidified mature mash and inoculating it with *Tetracoccus halophilus* and *Zygosacchariformis*, mixing uniformly to obtain a phase-inversion fermentation mash; cooling the phase-inversion fermentation mash and conducting anaerobic fermentation under air-isolated conditions to obtain the low-salt broad bean paste product. This invention solves the problems of insufficient umami and softening in low-salt fermentation by segmented salt control driving synergistic succession of microorganisms, combined with chemical regulation to protect enzyme activity and enhance texture, significantly improving the flavor and taste of the product.

This invention discloses a layered anti-aging composition and its application. It comprises 4-10 parts of *Vigna angularis* fruit extract, 0.01-0.2 parts of soluble collagen, and 10-20 parts of *Fucus vesiculosus* extract, all parts by weight. The composition of this invention employs a layered anti-aging skincare strategy ("layered" generally refers to different skin areas, i.e., "cellular layer-dermis-epidermal layer"). This composition can promote the expression of LOR (lower retinal occlusion) and the relative expression level of TGM5 (epidermis), increase the content of type I procollagen C-terminal peptide and elastin (dermis), enhance mitochondrial activity, and increase ATP content (cellular layer). That is, it works progressively from the epidermis to the dermis to the cellular layer, having a relevant effect on each layer of skin, and possessing the functions of enhancing skin elasticity, firming, anti-aging, and repairing the skin barrier.

This invention belongs to the field of saline-alkali land improvement technology, specifically relating to a compound microbial agent and its application in saline-alkali land improvement. This invention prepares a compound microbial agent for improving saline-alkali land using microorganisms such as Chaetomium coccidioides, Bacillus subtilis, Bacillus licheniformis, Bacillus belye, and humic acid. When applied to saline-alkali land improvement, this agent not only significantly improves the soil's physical and chemical properties but also significantly reduces the number of stunted cotton seedlings, increases cotton seed yield, and improves fiber quality.

This invention discloses a method for preparing bio-organic fertilizer that reduces the incidence of tobacco root-knot nematodes. The bio-organic fertilizer consists of a fermentation substrate, microbial inoculant, and earthworm castings in a mass ratio of 10:0.2-0.4:2-3. The invention uses Bacillus subtilis, Trichoderma harzianum, and Bacillus mucilaginosus in a mass ratio of 15-25:8-13:30-40 to prepare the microbial inoculant. The fermentation substrate and microbial inoculant are mixed and fermented. The fermentation substrate includes cow dung, wheat bran, straw, and earthworm castings. The bio-organic fertilizer of this invention can reduce the incidence of tobacco root-knot nematodes and also promotes tobacco root growth and increases tobacco leaf yield.

This invention discloses a herbal compound-based hair growth and anti-hair loss conditioning cream and its low-temperature preparation process, relating to the fields of cosmetics and scalp care technology. Specifically, it is a herbal compound-based hair growth and anti-hair loss conditioning cream and its low-temperature preparation process. The conditioning cream contains, by weight, herbal active ingredients such as ginseng, arborvitae leaf, duckweed, soapberry, angelica, chuanxiong, rosemary, ginger, asarum, and mulberry bark, as well as functional and matrix excipients such as sodium hyaluronate, cocoyl glucoside, carbomer 940, phenoxyethanol, and ethylhexylglycerin. Its preparation process employs low-temperature treatment throughout, including low-temperature extraction at room temperature to 50℃ and ultrasonic extraction at 200-800W, as well as low-temperature blending with low-speed stirring at 25-40℃, effectively preserving the active herbal ingredients. This product can improve scalp microcirculation, repair hair follicles, and regulate sebum secretion, suitable for various types of hair loss, while also relieving scalp itching and repairing damaged hair.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

The present application relates to a kind of pentosus PP90 for preventing and treating alcoholic nerve injury and its application, wherein pentosus PP90 is preserved in China Microbial Culture Collection Center, and the preservation number is CGMCC NO.32034.The fermentation product of the pentosus PP90 can degrade the alcoholicity of alcohol, can produce ethanol dehydrogenase and acetaldehyde dehydrogenase, can improve the viability of nerve cell, can regulate the expression of cytokine, can inhibit the activation of astrocyte, can regulate the expression of brain-derived neurotrophic factor, can improve the level of disturbed neurotransmitter, and can be used for preparing product for preventing and treating alcoholic nerve injury or improving alcoholic nerve injury.

This invention relates to the field of daylily cultivation technology, specifically to a method for cultivating daylilies resistant to leaf rust. The invention provides a method for cultivating daylilies resistant to leaf rust, comprising the following steps: Seed treatment: After sterilization, daylily seeds are soaked in a seed treatment agent and then placed at a constant temperature of 35-37℃ for 6-8 days to obtain germinated daylily seeds; Seedling management: The germinated daylily seeds are sown in a seedling substrate and cultivated until germination, obtaining daylily seedlings; Transplanting: The daylily seedlings are transplanted into soil treated with a soil treatment agent. This invention's cultivation method can improve the resistance of daylilies to leaf rust.

The present application belongs to the field of aquaculture and nutrition regulation technology, and particularly relates to a ursodeoxycholic acid preparation for inducing the group behavior of juvenile fish, a preparation method and application. The ursodeoxycholic acid preparation for inducing the group behavior of juvenile fish is composed of ursodeoxycholic acid and additives equivalent to 0% to 2% of the mass of ursodeoxycholic acid; the additives are cholic acid or a mixed agent, and the mixed agent is a mixture of taurine and tryptophan in a mass ratio of 1:1. After the ursodeoxycholic acid preparation for inducing the group behavior of juvenile fish is used to feed large yellow croaker juveniles, the juvenile fish olfaction can be stimulated, the group behavior of the juvenile fish can be induced, the growth of the large yellow croaker juveniles can be promoted, the metabolism can be promoted, and the immunity can be improved.

This invention relates to the field of biomedical technology, specifically disclosing a polypeptide and its application in the preparation of drugs for ischemic stroke. The amino acid sequence of the polypeptide is shown in SEQ ID NO. 1. This invention utilizes an in vivo tMCAO / R model and in vitro studies covering ischemic hypoxia (OGD) and neurotoxicity (MPP). + Multiple cell models of NL-PT, including those for cellular stress and hormonal stress (CORT), were used to reveal the application potential of the peptide NL-PT from multiple dimensions and levels. These results collectively demonstrate that NL-PT not only has a significant protective effect against the core pathological processes of ischemic stroke, but also exhibits broad-spectrum biological activity against various related injuries. In particular, its well-defined mechanism of anti-oxidative stress through scavenging reactive oxygen species (ROS) further highlights its multi-target intervention characteristics, providing solid and reliable experimental evidence for its development into a novel, multi-target therapeutic drug for ischemic stroke.

The application discloses a Saccharomyces cerevisiae capable of quickly assimilating ammonia and application thereof, and belongs to the technical field of microorganisms. The application provides a Saccharomyces cerevisiae hbx01 capable of quickly adsorbing ammonia gas and hydrogen sulfide gas and assimilating and absorbing ammonia, and the preservation number of the Saccharomyces cerevisiae is CCTCC NO: M 20221597. The glutamic acid dehydrogenase enzyme activity of the Saccharomyces cerevisiae is 38.6 U / mL. The Saccharomyces cerevisiae hbx01 is used in the treatment of ammonia gas and hydrogen sulfide, and after 24 h of treatment, the ammonia gas in the environment is reduced from 16.6 mg / m 3 to 8.2 mg / m 3 , the hydrogen sulfide is reduced from 0.26 mg / m 3 to 0.08 mg / m 3 , after 48 h, the ammonia gas in the environment is reduced to 4.8 mg / m 3 , and the hydrogen sulfide is reduced to 0.04 mg / m 3 .

The application provides a kind of preparation of red radish protoplast enzymatic hydrolysate and the preparation method and application of red radish protoplast, belong to the technical field of plant cytology.The enzymatic hydrolysate includes:1.0~1.5w / v% cellulase, 0.3~0.5w / v% dissociation enzyme and 0.3~0.5w / v% hemicellulase.The cellulase has the effect of degrading cellulose in plant cell wall, dissociation enzyme is used with cellulase, which is beneficial to separate plant tissue into single cell, and the main effect of hemicellulase is to degrade hemicellulose in plant cell wall.The enzymatic hydrolysate can synergistically promote the effect of red radish tissue cell wall lysis through the comprehensive effect of three enzymes, can better and faster destroy cell wall skeleton, is more conducive to the preparation of red radish protoplast and further maximally release protoplast, is conducive to improving the yield of protoplast, and can also keep the protoplast with high activity.

This invention discloses an extracellular polysaccharide DSP-1 from the shell of a deep-sea fungus, *Subseptospora*, and its applications. Didymella keratinophila The fermentation product of SCAU273 was isolated and purified to obtain an extracellular polysaccharide DSP-1. This polysaccharide has a molecular weight of 4116 Da, a bibranched structure, and is mainly composed of glucose polymers. Further studies showed that DSP-1 can enhance the activity and promote the proliferation of RAW 264.7 macrophages over a wide concentration range (25–400 μg / mL). Furthermore, at certain concentrations, it dose-dependently promoted the secretion of NO, TNF-α, IL-6, and IL-1β by RAW 264.7 macrophages, exhibiting good immunomodulatory activity against RAW 264.7 macrophages. It shows broad prospects for the production and widespread application of environmentally friendly marine biological agents.

The application discloses application of monardocin in preparation of a drug for treating thymus injury, and finds through cell experiments and animal experiments that the monardocin can be used for relieving acute thymus injury, regulating secretion of inflammatory factors in thymus epithelial cells and subgroups thereof, and can increase the number of immune cells, especially can significantly increase the number of thymus cells and cells in each subgroup in sepsis newborn mice, the number of cells in each subgroup of thymus epithelial cells, and the number of T lymphocytes and cells in each subgroup of the spleen. The application first proves that the natural product monardocin can relieve acute thymus atrophy in sepsis newborn mice, thereby improving the immunity of the body, and provides a brand-new natural drug intervention strategy for acute thymus injury induced by sepsis.

The application provides a genetically engineered bacterium for producing recovery promoting factor Rpf and application thereof, relates to the technical field of bioengineering, and belongs to prokaryotes. The nucleotide sequence of the genetically engineered bacterium is shown in SEQ ID NO. 1. The sequence is obtained by codon mutation and DNA sequence optimization of a wild-type bacterium recovery promoting factor of Micrococcus luteus in an original nucleotide sequence, and the optimized nucleotide sequence is named Delta Rpf. The original nucleotide sequence is shown in SEQ ID NO. 2. The purity of the Delta Rpf recombinant protein of the application can reach 93 %, which is much higher than the purity of the protein extracted by the prior art. The enzymatic activity of the Delta Rpf recombinant protein prepared by the genetically engineered bacterium constructed by the application is significantly higher than the enzymatic activity of the unmutated Rpf protein.

This invention discloses a fermentation process for Liushenqu (a traditional Chinese medicine fermentation product) and its application, specifically relating to the field of traditional Chinese medicine fermentation technology. The fermentation process involves pretreatment of raw materials, followed by inoculation of seed cultures of *Rhizopus oryzae*, *Bacillus subtilis*, and *Wickham's yeast* in a sterile substrate for aerobic fermentation in a specific ratio. Finally, after the environment has been acidified, seed cultures of *Lactobacillus plantarum* that have been induced are inoculated, and fermentation continues at a controlled temperature until the pH reaches 4.0-4.5. Fermentation is then stopped, yielding the fermented Liushenqu. This invention employs a method of rapid enzyme production and environmental acidification using molds and yeasts in the pre-fermentation stage, followed by deep transformation by lactic acid bacteria in the main fermentation stage. This approach simultaneously pursues high enzyme activity and high content of active ingredients, overcoming the limitations of existing technologies that focus only on a single indicator. Through the transformation effect of lactic acid bacteria, richer secondary metabolites are also produced, resulting in a more comprehensive spleen-strengthening and digestion-aiding effect.

The application discloses a method for culturing sweat gland cells to obtain a sweat gland organoid and application thereof, and belongs to the technical field of bioengineering, wherein the sweat gland cells can be cultured by using a three-dimensional culture medium provided by the application, and a sweat gland organoid with high cell activity and bidirectional differentiation potential is obtained; after being transplanted into a full-thickness injury mouse on the sole and back, the sweat gland organoid not only can promote the regeneration and function recovery of the sweat gland, but also can promote the healing of the skin wound, and can be used for preparing a cell product for repairing skin injury and recovering the function of an accessory organ, and has a wide application prospect.

This invention discloses a dry heat treatment method for tomato seeds to enhance seed vigor and extend storage life, relating to the field of tomato seed storage technology. The method includes: selecting mature, plump, and undamaged commercial tomato seeds with consistent maturity and maintaining appropriate moisture content; placing the pre-treated seeds in a single layer on a tray and placing it in a dry heat treatment device, performing dry heat treatment sequentially according to a preset program; comparing the germination indicators of dry heat-treated and untreated seeds to determine the effect of the dry heat treatment; and sealing and storing tomato seeds with qualified germination rates. This invention, through precisely controlled stepwise heating and high-temperature constant-temperature sterilization, effectively activates the seed's internal repair enzyme system while inhibiting metabolic processes that lead to seed deterioration, avoiding physical or physiological damage to the seeds caused by high-temperature treatment, and effectively preventing seed coat cracking, radicle burn, or excessive dehydration.

The application relates to the field of biotechnology, and particularly discloses a mutant strain Cbg61-T37, a co-expression strain Cbg61-T37-MPR1 and a construction method and application thereof. The mutant strain Cbg61-T37 is preserved in the China General Microbiological Culture Collection Center on August 18, 2025, has a preservation number of CGMCC No. 35649, and is classified and named as Pichia pastoris. Pichia pastoris The mutant strain Cbg61-T37 provided by the application has an enzyme hydrolysis activity of 62.9 U / mg, which is increased by 43.8% compared to the original strain. Therefore, the mutant strain Cbg61-T37 is more conducive to catalysis and synthesis of the rare ginsenoside CK.

The application discloses lactobacillus plantarum WH021 and application thereof in prevention and / or alleviation of neuroinflammation, and belongs to the technical field of microorganisms. The lactobacillus plantarum WH021 is obtained by separation and purification from healthy infant feces, is a probiotic with the effect of alleviating neuroinflammation, and has good application potential in development of food, health products and drugs for preventing brain inflammation related diseases of middle-aged and old people caused by chronic inflammation of the body.

This invention relates to a strain of *Bacillus pseudofungus* and its applications, belonging to the field of biotechnology. The preservation number of the *Bacillus pseudofungus* is CGMCC No. 37620. It has the functions of producing siderophores, cellulase, urease, and protease, as well as promoting crop growth, enhancing crop root vitality, and preventing and controlling anthracnose in apples, grapes, strawberries, strawberries, apple ring rot, maize large leaf spot, maize small leaf spot, quinoa leaf spot, and maize ear rot.