79results about How to "Increase vitality" patented technology

The invention belongs to the field of skin care and skin disease treatment, and provides a skin care composition and application thereof, and the skin care composition is in a liquid form and comprises recombinant collagen and glycolic acid. The specific low-concentration glycolic acid in the skin care composition disclosed by the invention ensures the mild keratin metabolism capability; the recombinant collagen with high transdermal property can quickly permeate into the deep layer of the skin, and can immediately provide a repairing raw material for the skin while the glycolic acid acts, so that discomforts such as redness and stabbing pain are remarkably relieved, and synchronous skin care and repairing is realized.

The present application provides a kind of prevention and control method of green moss in water lily planting pool, specifically belongs to water quality control technical field.The prevention and control method of green moss in water lily planting pool described in the present application includes the following steps: before planting water lily, water injection, water level is controlled at 15~20cm, copper sulfate is applied to kill green moss and its spores, keep water body not to flow during disinfection;After disinfection, apply sodium thiosulfate, and plant water lily;After planting water lily, keep replenishing water, control water level to 40~50cm, when green moss first outbreak, apply mineral fulvic acid potassium and composite bacillus;Subsequent green moss outbreak, apply mineral fulvic acid potassium, or apply mineral fulvic acid potassium and composite bacillus.The prevention and control method described in the present application is safe and efficient, which can quickly and effectively remove green moss without inhibiting the growth of water lily, and also does not affect the harvest of water lily.

The present application relates to the field of plant tissue culture technology, and particularly discloses a lily stem cell abundance ex vivo regulation method. The lily bulb is used as the starting material, stem cell magnitude amplification and regulation culture can produce lily tissue organ materials with high stem cell abundance. The present application provides sterile lily materials with high stem cell abundance, which has the characteristics of strong regenerative ability, high proliferation rate, etc., is the basis for protoplast preparation and high-efficiency regeneration, and is suitable for different lily varieties, and provides technical support for cell engineering breeding, seedling scale breeding, etc.

This invention discloses a method for producing crop cultivation substrate using sugarcane filter mud and sugarcane pith as main raw materials, comprising the following steps: (1) dissolving and diluting 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylic acid in water to obtain a solution; (2) mixing sugarcane slurry, sugarcane pith, the 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylic acid solution, fermentation agent, and improver, adjusting the moisture content, and then composting and fermenting. The compost is turned over every few days and continuously composted and fermented for several days to obtain the crop cultivation substrate. The crop cultivation substrate produced by this invention is of excellent quality, allowing direct planting of plants without root burn. Therefore, this invention, using sugarcane filter mud and sugarcane pith as main raw materials to prepare and produce crop cultivation substrate, can effectively solve the problem of comprehensive utilization of sugarcane filter mud and improve product sales.

The invention discloses a freckle-removing and whitening mask which is prepared from the following raw materials: chrysanthemum, pearl, radix angelicae, almond, poria cocos, rhizoma atractylodis macrocephalae, peach kernel, ginseng, mung bean, bletilla striata, cortex moutan, rhodiola rosea, silkworm larva, Chinese yam, honeysuckle and the like. The facial mask is a pure traditional Chinese medicine facial mask, has remarkable curative effects on whitening, removing freckles, removing acnes and minimizing pores, and can effectively reduce facial skin damage, effectively inhibit growth of facial germs, promote vigor of subcutaneous capillaries of the damaged face, improve metabolism of the skin and enable the facial skin to reappear in a compact and refined state. In addition, the facial mask can enhance the ability of facial skin to resist invasion and stimulation of external germs, form a natural protective barrier for the facial skin, well repair, moisten and care the facial skin, and keep the facial skin comfortable, beautiful and healthy for a long time. Pure traditional Chinese medicine raw materials are selected, chemical components are not added, and harm to facial skin and the body is avoided.

This invention discloses a method for preparing and applying a sodium borohydride-modified self-supporting anode for AEM water electrolyzers. Firstly, an amorphous ternary metal hydroxyl oxide precursor is grown in situ on a nickel substrate via room temperature liquid phase deposition. The process involves the presence of Co in solution. 2+ Fe 3+ Ni ions are introduced through slight corrosion of the nickel foam matrix. 2+ The ions, the three, and the OH- produced by hydrolysis ⁻ Co-deposition occurs under the influence of dissolved oxygen, leading to the self-assembly of amorphous nanosheet structures on the substrate surface. Subsequently, a mild chemical reduction treatment with sodium borohydride solution is used to obtain a structurally stable and high-performance self-supporting electrode. The method described in this embodiment is mild, simple, low-cost, and has good versatility.

The invention discloses an efficient induction method of leontopodium leontopodum adventitious roots, and belongs to the technical field of plant tissue culture. According to the efficient induction method for the leontopodium leontopodum adventitious roots, a leontopodium leontopodum sterile system and sterile seedlings are established, calluses are induced, rooting of tissue culture seedlings is promoted, browning is inhibited, and the adventitious roots extend; then the adventitious roots are induced through three ways, and the rooting induction rate of the leontopodium leontopodum adventitious roots is remarkably increased; the problems of leontopodium leontopodum resource shortage and large market demand are solved; a uniform, rapid and feasible culture is induced; the leontopodium leontopodum adventitious root induction method can be industrially produced, and sufficient raw materials are provided for extraction of leontopodium leontopodum flavone; by means of the method, large-scale culture of the leontopodium leontopodum adventitious roots is achieved, a large number of medicine and cosmetic raw materials can be obtained at low cost in a short time, and an integrated production system can be formed with the medicine industry and the cosmetic industry.

The application discloses a prenylated polyketide compound, a preparation method and application thereof, and the prenylated polyketide compound is separated from rice fermented with Xylaria nigriplicata. The neuroprotective effect of the prenylated polyketide compound on glutamate-induced excitotoxicity of PC12 cells is clarified through comprehensive evaluation of cell viability, apoptosis and key oxidative stress markers.

This invention provides an oyster peptide and its application in enhancing immunity. The oyster peptide has a novel amino acid structure. The oyster peptide can enhance the activity of immune cells, promote the proliferation of immune cells, promote the development and maturation of immune organs, regulate the in vivo immune environment, promote the secretion of immunoglobulins, and improve humoral immunity.

This invention relates to the field of broad bean paste technology and discloses a low-salt broad bean paste production process based on the synergistic fermentation of compound microorganisms. The process includes: uniformly mixing broad bean koji with drained chili embryos to obtain a solid mixture; spraying an A-phase initiation conditioning solution onto the solid mixture and inoculating it with *Lactobacillus plantarum*, mixing until moistened to obtain a primary fermentation mash; controlling the primary fermentation mash to undergo constant-temperature fermentation until the pH value drops to a preset acidic range to obtain an acidified mature mash; injecting a B-phase hypertonic regulating solution into the acidified mature mash and inoculating it with *Tetracoccus halophilus* and *Zygosacchariformis*, mixing uniformly to obtain a phase-inversion fermentation mash; cooling the phase-inversion fermentation mash and conducting anaerobic fermentation under air-isolated conditions to obtain the low-salt broad bean paste product. This invention solves the problems of insufficient umami and softening in low-salt fermentation by segmented salt control driving synergistic succession of microorganisms, combined with chemical regulation to protect enzyme activity and enhance texture, significantly improving the flavor and taste of the product.

A flowerpot body (1) is provided with at least one larger planting hole (2) communicated with a smaller water permeable hole (3), and the overlook projection area of an aerial root attachment platform (5) on the periphery of the planting hole (2) is obviously larger than that of the planting hole (2). The environment of the platform (5) is moist, the dendrobe (8) is rapidly induced to grow white aerial roots (4), and the aerial roots (4) are fixed on the steamed bun-shaped red platform (5), extend in a radial shape, extend all around, are like lightning and are sky-spectacular, strong vitality is shown, and the ornamental value of the potted dendrobe is greatly improved.

This invention discloses a layered anti-aging composition and its application. It comprises 4-10 parts of *Vigna angularis* fruit extract, 0.01-0.2 parts of soluble collagen, and 10-20 parts of *Fucus vesiculosus* extract, all parts by weight. The composition of this invention employs a layered anti-aging skincare strategy ("layered" generally refers to different skin areas, i.e., "cellular layer-dermis-epidermal layer"). This composition can promote the expression of LOR (lower retinal occlusion) and the relative expression level of TGM5 (epidermis), increase the content of type I procollagen C-terminal peptide and elastin (dermis), enhance mitochondrial activity, and increase ATP content (cellular layer). That is, it works progressively from the epidermis to the dermis to the cellular layer, having a relevant effect on each layer of skin, and possessing the functions of enhancing skin elasticity, firming, anti-aging, and repairing the skin barrier.

The invention provides a silk fibroin composition for injection and a preparation method thereof, and belongs to the technical field of medical cosmetology. The silk fibroin composition is prepared from the following components in percentage by mass: 0.1 to 0.7 weight percent of hyaluronic acid or salt thereof, 0.1 to 10 weight percent of silk fibroin and the balance of phosphate buffer solution. The silk fibroin composition is used for water-light injection, can promote collagen production and inhibit collagen fibrosis, does not have adverse reactions such as skin allergy, hardening and the like, and has the effects of quickly improving the dry state of skin, fading fine wrinkles or wrinkles, replenishing water, preserving moisture and tightening the skin.

The invention discloses a wild lespedeza seed dormancy breaking and germination accelerating method, and belongs to the technical field of plant seed breeding. The method comprises the steps of physical skin breaking, surface cleaning and disinfection, warm water seed soaking, chemical dormancy breaking, gradient germination accelerating, batch sowing and intelligent water and fertilizer management. Aiming at the problems of low natural germination rate, irregular seedling emergence and the like caused by double obstacles of hard seed coats and shallow physiological dormancy of wild lespedeza seeds, the method synchronously breaks the double dormancy obstacles through a multi-stage synergistic mechanism of constructing a moisture permeation channel through physical coat breaking, breaking dormancy through chemical dormancy breaking and simulating environmental signals through gradient germination acceleration, and the germination rate of the wild lespedeza seeds is improved. The seed germination rate and the seedling emergence uniformity are effectively improved, and mildewing is inhibited. The method is stable in process, high in operability and suitable for large-scale seedling raising of the wild lespedeza, and reliable technical support is provided for ecological restoration engineering.

This invention belongs to the field of saline-alkali land improvement technology, specifically relating to a compound microbial agent and its application in saline-alkali land improvement. This invention prepares a compound microbial agent for improving saline-alkali land using microorganisms such as Chaetomium coccidioides, Bacillus subtilis, Bacillus licheniformis, Bacillus belye, and humic acid. When applied to saline-alkali land improvement, this agent not only significantly improves the soil's physical and chemical properties but also significantly reduces the number of stunted cotton seedlings, increases cotton seed yield, and improves fiber quality.

The application provides a boar semen cryopreservation agent and an application method; the boar semen cryopreservation agent comprises alpha-amylase, and the content of the alpha-amylase is 10-1000 mu g / L. The application method of the cryopreservation agent is as follows: centrifuging the boar semen after collection and pretreatment, removing supernatant after centrifugation; adding a refrigerant, re-suspending the sperm, and then placing the sperm in a 4 DEG C refrigerator and standing for 3-4 hours; adding a freezing liquid and fully mixing; filling the semen into a thin tube at 4 DEG C, uniformly laying the thin tubes in a freezing rack, and then precooling on a liquid nitrogen surface and then placing in liquid nitrogen for freezing. The application has the advantages that: the overall components of the cryopreservation agent are simple, the cost is low, and the overall cost performance is high; due to the addition of alpha-amylase in the cryopreservation agent, the storage quality of the boar frozen semen can be significantly improved; and the activity, acrosome integrity, DNA integrity, mitochondrial activity and other physical and chemical properties of the boar frozen semen are effectively improved.

The application provides a recombinant polymyxin enzyme and a preparation and application method thereof, an amino acid sequence of the recombinant polymyxin enzyme is shown as SEQ ID NO. 1, and the recombinant polymyxin enzyme is obtained through site-directed mutation on an amino acid sequence of a wild-type polymyxin enzyme derived from Brevibacillus laterosporus. The recombinant polymyxin enzyme can specifically recognize and cut a peptide bond between a tripeptide side chain and a cyclic heptapeptide ring in a polymyxin structure and a peptide bond between threonine and diaminobutyric acid inside the cyclic heptapeptide ring, and further can specifically degrade or neutralize the recombinant polymyxin enzyme of polymyxin.

This invention discloses a method for preparing bio-organic fertilizer that reduces the incidence of tobacco root-knot nematodes. The bio-organic fertilizer consists of a fermentation substrate, microbial inoculant, and earthworm castings in a mass ratio of 10:0.2-0.4:2-3. The invention uses Bacillus subtilis, Trichoderma harzianum, and Bacillus mucilaginosus in a mass ratio of 15-25:8-13:30-40 to prepare the microbial inoculant. The fermentation substrate and microbial inoculant are mixed and fermented. The fermentation substrate includes cow dung, wheat bran, straw, and earthworm castings. The bio-organic fertilizer of this invention can reduce the incidence of tobacco root-knot nematodes and also promotes tobacco root growth and increases tobacco leaf yield.

The invention belongs to the technical field of natural medicinal chemistry, and particularly relates to a novel benzofuran compound, namely, uncinenitone, separated from wingceltis, a pharmaceutically acceptable isomer of the uncinenitone, a wingceltis extract containing the compound, and a preparation method and application of the uncinenitone. The unwanone has a chemical structure as shown in a formula I, R1 is methoxy or hydroxyl, and R2 is a hydrogen atom or methoxy. The invention also provides a method for extracting the compound or the extract from the stem of the wingceltis, and purifying by combining pH gradient extraction with silica gel column chromatography, semi-preparative high performance liquid chromatography and gel chromatography to obtain the compound or the extract. The wingcelanone, the isomer of the wingcelanone, and the wingcelanone extract or the pharmaceutical composition containing the wingcelanone show remarkable gastric mucosa protection activity in an in-vitro cell model.

The invention discloses a composite extract for improving sleep and a preparation method thereof, and belongs to the technical field of plant extraction. According to the invention, four medicinal materials of spina date seed, lily, lotus seed and poria cocos which are homologous in medicine and food and have the effect of soothing the nerves are scientifically combined, and the composite extract for improving sleep is obtained by adopting a specific extraction process; according to the technology, active ingredients are efficiently released through the synergistic effect of cellulase, pectinase and acid protease, and the yield of gamma-aminobutyric acid and other sedative substances is remarkably increased through a co-fermentation system of lactobacillus plantarum and saccharomyces cerevisiae; lipid-soluble effective components are extracted by adopting glycyrrhizic acid and rhamnolipid assisted ultrasonic extraction technology, and finally, embedding protection of the active components is realized through a chitosan oligosaccharide-soybean lecithin system, so that thermal degradation and oxidation loss in the processing process are reduced, and compared with a traditional single extraction method, the preparation method has the advantages that the cost is reduced; according to the method, high-yield gamma-aminobutyric acid, jujuboside, triterpenic acid and other active ingredients can be obtained at the same time, and the effect of the composite extract is improved.

This invention discloses a method for cultivating hardy kiwifruit, comprising the following steps: S1 Cultivating male 'Jinmi Rootstock No. 1' seedlings; S2 Grafting, starting in mid-to-late February, high-position grafting is performed on the 'Jinmi Rootstock No. 1'; First, a grafting method with a grafting diameter of 0.8 cm is used: the scion is 5-7 cm long, each segment retains 2-3 buds, and the lower end is cut into an oblique surface; the rootstock is cut at a diameter of 0.8 cm, then longitudinally cut, with the cut size similar to that of the scion; then the cambium layers of the scion and rootstock are aligned and tied tightly with a thin film strip. Beneficial effects: 'Jinmi Rootstock No. 1' can increase the average yield of hardy kiwifruit cultivars by 25.7%, showing a significant yield increase; 'Jinmi Rootstock No. 1' has a very well-developed root system, strong vitality, and can be directly propagated by cuttings, making seedling cultivation easy; it improves the adaptability of hardy kiwifruit cultivation and reduces the rate of tree death caused by waterlogging.

This invention discloses a herbal compound-based hair growth and anti-hair loss conditioning cream and its low-temperature preparation process, relating to the fields of cosmetics and scalp care technology. Specifically, it is a herbal compound-based hair growth and anti-hair loss conditioning cream and its low-temperature preparation process. The conditioning cream contains, by weight, herbal active ingredients such as ginseng, arborvitae leaf, duckweed, soapberry, angelica, chuanxiong, rosemary, ginger, asarum, and mulberry bark, as well as functional and matrix excipients such as sodium hyaluronate, cocoyl glucoside, carbomer 940, phenoxyethanol, and ethylhexylglycerin. Its preparation process employs low-temperature treatment throughout, including low-temperature extraction at room temperature to 50℃ and ultrasonic extraction at 200-800W, as well as low-temperature blending with low-speed stirring at 25-40℃, effectively preserving the active herbal ingredients. This product can improve scalp microcirculation, repair hair follicles, and regulate sebum secretion, suitable for various types of hair loss, while also relieving scalp itching and repairing damaged hair.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

The invention belongs to the technical field of gene engineering, and particularly relates to a 3-ketosteroid-delta1-dehydrogenase mutant with improved heat resistance and an application of the 3-ketosteroid-delta1-dehydrogenase mutant. The 3-ketosterone-delta1-dehydrogenase AuKsdD gene derived from arthrobacter urate oxydans is subjected to structural analysis and site-directed mutagenesis design, and a mutant with higher catalytic activity and remarkably enhanced heat resistance compared with a wild type AuKsdD is obtained through directed mutation of key sites E244, K267, T301 and A335. The mutant can keep high catalytic efficiency for a long time under a high-temperature condition, and is suitable for industrial synthesis of steroid drug raw materials.

The application provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: (1) crushing; (2) extraction; (3) standing and separation; (4) freezing and filtration, so that the medicinal liquor is obtained. The medicinal liquor is obtained by adopting the steps of batch crushing, mixed extraction, standing, freezing and separating supernatant and suspension by using different separation methods to extract traditional Chinese medicine ingredients. The medicinal liquor can better improve the space exploration ability of aging mice caused by D-galactose, reduce malondialdehyde (MDA), improve superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and has significant antioxidant and anti-aging effects.

The present application relates to a kind of pentosus PP90 for preventing and treating alcoholic nerve injury and its application, wherein pentosus PP90 is preserved in China Microbial Culture Collection Center, and the preservation number is CGMCC NO.32034.The fermentation product of the pentosus PP90 can degrade the alcoholicity of alcohol, can produce ethanol dehydrogenase and acetaldehyde dehydrogenase, can improve the viability of nerve cell, can regulate the expression of cytokine, can inhibit the activation of astrocyte, can regulate the expression of brain-derived neurotrophic factor, can improve the level of disturbed neurotransmitter, and can be used for preparing product for preventing and treating alcoholic nerve injury or improving alcoholic nerve injury.

The invention discloses a method for separating cochlea related cells in situ based on a patch clamp system. The method comprises the following steps: contacting an electrode with a target single cell under a microscope, and carrying out in-situ grabbing on the target single cell through negative pressure adsorption; the target single cell and surrounding cells are kept in a connection state before in-situ grabbing; before the electrode approaches the target single cell, positive pressure is continuously applied, and when the electrode is in contact with the surface of the target single cell, the positive pressure is instantly stopped and switched to negative pressure. According to the method provided by the invention, high-quality cochlea related cells can be obtained in situ for deep sequencing analysis, and the method can be used for basic research and accurate diagnosis and treatment of clinical hearing impairment, and has a good application prospect.

This invention relates to the field of daylily cultivation technology, specifically to a method for cultivating daylilies resistant to leaf rust. The invention provides a method for cultivating daylilies resistant to leaf rust, comprising the following steps: Seed treatment: After sterilization, daylily seeds are soaked in a seed treatment agent and then placed at a constant temperature of 35-37℃ for 6-8 days to obtain germinated daylily seeds; Seedling management: The germinated daylily seeds are sown in a seedling substrate and cultivated until germination, obtaining daylily seedlings; Transplanting: The daylily seedlings are transplanted into soil treated with a soil treatment agent. This invention's cultivation method can improve the resistance of daylilies to leaf rust.

The invention discloses an instant composition for assisting in improving memory and application of the instant composition. The traditional Chinese medicine composition comprises the following raw materials in parts by mass: 5-10 parts of thunberg fritillary bulb, 3-10 parts of allium macrostemon, 1-3 parts of turmeric, 10-20 parts of salvia miltiorrhiza, 5-10 parts of red peony root, 3-10 parts of peach kernel, 2-10 parts of safflower, 3-10 parts of immature bitter orange, 3-10 parts of mangnolia officinalis, 10-15 parts of rhizoma smilacis glabrae, 10-30 parts of prepared rehmannia root, 5-10 parts of Chinese yam, 4-10 parts of dogwood, 3-10 parts of rhizoma alismatis, 3-10 parts of radix achyranthis bidentatae, 3-10 parts of poria cocos and 3-10 parts of rhizoma atractylodis. Through the scientific compatibility of the selected 21 traditional Chinese medicines and the modern extraction and dosage form process, the multi-component and multi-target synergistic memory improvement is realized, and meanwhile, the traditional Chinese medicine composition has natural safety, efficient absorption and taking convenience.

This invention discloses a plant-based composition for promoting keratinocyte renewal and refining skin, and its application, belonging to the field of cosmetic technology. The composition comprises pomegranate fruit extract, kale leaf extract, Dendrobium officinale stem extract, and phytosterols in a specific ratio. The Dendrobium officinale stem extract is obtained using a choline chloride-oxalic acid eutectic solvent combined with microfluidic extraction. In this composition, pomegranate fruit extract promotes keratinocyte regeneration, kale leaf extract accelerates the shedding of dead skin cells, and the two synergistically regulate keratinocyte metabolism. Dendrobium officinale stem extract enhances skin's moisturizing ability, softens keratinocytes, and creates a moist renewal environment. Phytosterols repair the skin barrier and enhance the vitality of basal cells. These four components form a complete chain of action involving deep activation, metabolic regulation, and barrier repair, endogenously promoting keratinocyte renewal. It also provides exfoliation, skin refinement, long-lasting moisturizing, and barrier repair effects, and is gentle and non-irritating, making it suitable for sensitive skin.