231 results about "Covalent binding" patented technology

The invention relates to the technical field of biomedicine, in particular to a cross-linked modified artificial valve and a preparation method and application thereof. The preparation method comprises the following steps: firstly, preparing a novel compound POSS-PEG-NHS, and carrying out esterification reaction on a-NHS group of the compound and a-NH2 group on the surface of the decellularized porcine valve to realize covalent binding; then, an inherent reducing agent glutathione in an organism is added, and the cross-linked modified artificial valve PPN-GSH-AV is obtained. The valve shows excellent cell safety and blood compatibility, and has obvious advantages compared with a traditional glutaraldehyde cross-linked valve. In-vivo experiments show that the PPN-GSH-AV has high biological safety and histocompatibility, and can effectively reduce immunological rejection. In addition, the valve can relieve oxidative stress of macrophages and promote M2 type transformation, so that inflammatory response is relieved. In clinical application, the PPN-GSH-AV shows a good anti-inflammatory effect and excellent anti-calcification performance, in-vitro pulsating flow test results meet international standards, and the PPN-GSH-AV has clinical transformation potential and wide application prospects.

The present invention relates to a compound binding to an endogenous receptor, said compound comprising (i) an oligopeptide comprising a dipeptide with Trp being the C-terminal amino acid of said dipeptide, wherein said Trp is replaced with an α-amino acid Xaa2, whereby the stability in serum or plasma of the peptide bond connecting Xaa2 to the N-terminally adjacent amino acid is increased as compared to the peptide bond connecting Trp to the N-terminally adjacent amino acid in an otherwise identical compound; and (ii) a moiety capable of generating therapeutically effective radiation, said moiety being covalently bound to said oligopeptide.

Provided are polymer compositions made by a process comprising: (a) providing a first reactive composition containing: (i) a polymerization initiator that is capable, upon a first activation, of forming two or more free radical groups, at least one of which is further activatable by subsequent activation; (ii) one or more ethylenically unsaturated compounds; and (iii) a crosslinker; (b) subjecting the first reactive composition to a first activation step such that the first reactive composition polymerizes therein to form a crosslinked substrate network containing a covalently bound activatable free radical initiator; (c) contacting the crosslinked substrate network with a grafting composition containing one or more ethylenically unsaturated compounds, wherein the contacting is conducted under conditions such that the grafting composition penetrates into the crosslinked substrate network; and (d) activating the covalently bound activatable free radical initiator at one or more selective regions of the crosslinked substrate network such that the grafting composition polymerizes with the crosslinked substrate network at the selective regions.

The invention discloses an electrochemical biosensor for detecting mycobacterium tuberculosis. The sensor takes a porous membrane as a base material, the inner wall of a pore channel of the porous membrane is co-modified with a polydopamine (PDA) and conductive carbon material composite layer, and an amino-modified specific capture probe based on a mycobacterium tuberculosis ESAT-6 gene is fixed on a modification layer through covalent binding. When a target gene is captured by a probe, the volume effect of the target gene causes blockage of a porous channel of the porous membrane, so that electrochemical signals (such as current) of the sensor are changed, and high-sensitivity and specific detection of mycobacterium tuberculosis is realized. According to the invention, a porous structure, biological probe specific fixation and an electrochemical signal conversion technology are combined, and a novel efficient tool is provided for rapid diagnosis of tuberculosis.

The present invention belongs to the field of nano drug preparations, and discloses a multifunctional nanozyme platform and its preparation method and application, which is mainly composed of nanozymes, carrier materials and targeting substances. The synthesis of nanozymes, the loading of nanozymes on carrier materials and the modification of their surface targeting substances are carried out by methods such as stirring synthesis, coprecipitation, strong acid oxidation, chemical reduction, covalent bonding, solvent thermal method, physical adsorption, etc. The targeting substance on the surface of the nanozyme platform can be accurately and efficiently positioned to the inflammation site, and the acidic microenvironment of the inflammation site causes the targeting substance and the carrier material to undergo structural depolymerization, thereby releasing a variety of nanozymes, which not only realize the self-cascade and synergistic catalytic enhancement of enzymes at the inflammation site, but also form a substrate self-circulation catalytic system, thereby achieving the effect of efficiently alleviating and treating various inflammation-related diseases.

A precursor compound and a radioligand for cancer diagnosis and treatment comprises a high-affinity ligand PL for a cancer- associated protein conjugated to a covalent warhead CW for strong or covalent binding to an amino acid sidechain of the cancer-associated protein and a chelator Ch for complexation of a radioisotope or a leaving group LR for substitution with a radioisotope, wherein the covalent warhead CW is configured for quasi-covalent ionic binding using a sulfonic acid group or for covalent binding using a sulfur fluoride exchange (SuFEx) group, a benzotriazole or N-methyl-N- arylmethanesulfonamide leaving group or a malolactone group for strain-release alkylation.

The invention belongs to the technical field of biological medicine, and particularly relates to hyaluronic acid nano-microspheres as well as a preparation method and application thereof. According to the preparation method of the hyaluronic acid nano-microspheres, the hyaluronic acid nano-microspheres are prepared through interaction of nucleic acid, basic amino acid and hyaluronate in a specific proportion, the preparation process is optimized, and the prepared hyaluronic acid nano-microspheres are high in hyaluronic acid adding amount, high in skin permeability and good in skin care effect. The stability is good, and the application requirements in transdermal drug delivery drugs, skin care products and other products are met. Meanwhile, a non-covalent binding mode is adopted for preparing the nano-microspheres, the preparation operation is simple, the gelling property of the hyaluronate is effectively improved, the sticky feeling under high content is improved, and the application scene of hyaluronic acid in the field of skin care products is widened.

The invention discloses a PROTAC chimera for targeted degradation of an ROR [gamma] t receptor and application of the PROTAC chimera, the structure of the chimera is RW-L-Re, Re is a ligand capable of being combined with E3 ubiquitin ligase, L is a linking group covalently combined with at least one Re and at least one RW, and Rw is a target protein ROR [gamma] t binding ligand and is selected from one of the following structures. A series of PROTAC chimeras capable of degrading an ROR gamma t receptor in a targeted manner are designed and synthesized for the first time, a ternary complex is formed mainly by combining a target protein ligand and an E3 ubiquitin ligase ligand with POI and E3 ligase respectively, and then the POI is labeled with a ubiquitination tag and is further degraded by proteasome. Experimental results prove that the PROTAC chimera designed by the invention has excellent ROR [gamma] t receptor degradation activity, and can be used for preparing related drugs for treating autoimmune diseases or tumors.

The invention belongs to the field of environmental biotechnology and nano material application, and relates to a method for accelerating microorganisms to reduce selenite based on immobilized riboflavin. Aiming at the problems of poor stability, difficulty in recovery, non-repeated use and the like of dissolved riboflavin serving as an electron shuttle, riboflavin is grafted on the surface of a multi-walled carbon nanotube through a covalent binding method, and immobilized riboflavin capable of being recycled is constructed. The material can accelerate extracellular electron transfer of shewanella MR-1, and toxic sodium selenite is efficiently reduced into low-toxicity or non-toxic elemental selenium. Results show that the immobilized riboflavin not only maintains high reduction activity of riboflavin, but also has excellent cyclic utilization performance, still maintains high reduction rate after being repeated for multiple times, and effectively reduces operation cost and resource loss. According to the invention, an efficient, economical and sustainable application scene is provided for a microorganism-electron shuttle combined remediation technology, and a new thought is also provided for application of a functional nano material in an environmental biotechnology.

The invention belongs to the technical field of lipase immobilization, and particularly relates to immobilized lipase and a preparation method thereof. The preparation method comprises the following two steps: (I) preparation of activated amino type silica gel resin: mixing amino type silica gel resin, a nonionic surfactant 1308 and deionized water according to a mass ratio of (1000-1100): (2000-2200): (5-8), stirring at 1-5 DEG C for 10-15 minutes, adding cyanuric chloride, dripping a 30-35 wt% sodium hydroxide solution, reacting, and centrifuging; (II) preparation of immobilized lipase: mixing the activated resin, a surfactant, deionized water and a lipase solution, dropwise adding an alkaline solution at 40-45 DEG C under the condition that the pH is 5.0-5.5, and centrifuging, washing and drying after reaction. The obtained immobilized lipase is firm in covalent binding, the enzyme activity is greater than or equal to 50% after the immobilized lipase is repeatedly used for 6 times, the acid-base tolerance and the storage stability are excellent, and the immobilized lipase is suitable for industrial catalysis in multiple industries.

A novel active agent-eluting hemostatic agent and methods of use and manufacture thereof are presented. A polymer, such as gelatin, is used as the base for a hemostatic agent. The gelatin is crosslinked with a chemical crosslinker, such as a carbodiimide, in the presence of an active agent. The active agent may be an anesthetic or antimicrobial, such as an antibiotic. The novel process allows for the active agent to be both covalently bound to the gelatin as well as be trapped within cages in the gelatin that are formed from the crosslinking. This dual measure allows for controlled and sustained release of the active agent from the hemostatic agent to reduce surgical site infections.

A method is provided for treating a subject with cancer using a recombinant polypeptide comprising a DRα1 domain containing a glutamine residue at the position corresponding to amino acid 45 of SEQ ID NO: 1 or SEQ ID NO: 2, or an antigenic peptide covalently bound to a portion thereof. In some cases, the subject has a tumor that is resistant to immune checkpoint blockade treatment and / or does not express a BRAF mutation. The present invention provides, for example, a method for treating a subject with cancer, comprising administering to the subject a therapeutically effective amount of a recombinant polypeptide comprising a DRα1 domain containing a glutamine residue at the position corresponding to amino acid 50 of SEQ ID NO: 1 or SEQ ID NO: 2, or an antigenic peptide covalently bound to a portion thereof; or a nucleic acid encoding the recombinant polypeptide.

The application discloses a kind of detection flavonoid compound magnetic composite material, preparation, detection method and the application of preparation standard substance, belong to analytical chemistry technical field.Magnetic composite material includes magnetic nanoparticle core, double lanthanide series metal organic framework (MOF) first shell and covalent organic framework (COF) second shell successively coated on its surface, and phenylboronic acid group grafted on the surface of second shell.The material uses the specific covalent binding of phenylboronic acid and the characteristic ortho-diphenol hydroxyl group of flavonoid compound to realize efficient capture and enrichment;Through double lanthanide series MOF shell, promote energy transfer, COF shell provides high specific surface area and ordered diffusion channel, and magnetic core is convenient for rapid separation, which realizes high selectivity identification of flavonoid compound.The material has the advantages of high sensitivity, strong anti-matrix interference ability, rapid and simple detection, etc., and is suitable for accurate analysis of flavonoid compound in complex sample and development of related standard substance.

Provided are polymer compositions made by a process comprising: (a) providing a first reactive composition containing: (i) a polymerization initiator that is capable, upon a first activation, of forming two or more free radical groups, at least one of which is further activatable by subsequent activation; (ii) one or more ethylenically unsaturated compounds; and (iii) a crosslinker; (b) subjecting the first reactive composition to a first activation step such that the first reactive composition polymerizes therein to form a crosslinked substrate network containing a covalently bound activatable free radical initiator, (c) combining the crosslinked substrate network with a second reactive composition containing one or more ethylenically unsaturated compounds; and (d) activating the covalently bound activatable free radical initiator of the crosslinked substrate network such that the second reactive composition polymerizes therein with the crosslinked substrate network to form a grafted polymeric network and a byproduct polymer. Also provided are precursors to the polymer compositions, processes for preparation of the polymer compositions, and methods of using the polymer compositions, for instance in medical devices.

Methods of analyzing a fecal sample containing parasitic material and non-parasitic material are disclosed. The method may include combining the fecal sample with a flotation medium in a container. The method may further include at least partially separating the parasitic material and non-parasitic material in the flotation medium. The method may further include extracting at least a portion of the parasitic material from the flotation medium. The method may further include staining or labeling the extracted parasitic material with a fluorochrome comprising a functional group reactive with a functional group of the parasitic material, where the fluorochrome may bind to the parasitic material via a covalent bond. The method may further include placing the labeled parasitic material within an imaging unit for imaging and analysis of the labeled parasitic material.

A method for producing hydrophobic dry surface-modified fibrillated cellulose to improve dispersion in hydrophobic matrices includes: providing micro-fibrillated cellulose suspension of 5-30 wt % in water; adding a surfactant to the suspension with a mass ratio of surfactant to micro-fibrillated cellulose suspension of 0.1-2.0 wt %; adding a surface modifier to the suspension with a mass ratio of surface modifier to micro-fibrillated cellulose dry matter of 20-300 wt %, with the surface modifier obtained through a chemical reaction of a silane compound with a phenolic compound to create covalent bonds between the silane compound and the phenolic compound; covalently binding the surface modifier to the micro-fibrillated cellulose via (i) hydrolysis of the surface modifier to form reactive silanol groups and (ii) condensation of the silanol groups with available OH-groups of the micro-fibrillated cellulose; adding a plasticizer to the suspension of surface modified micro-fibrillated cellulose; drying the suspension.

The invention belongs to the technical field of hydrogel preparation, and relates to a preparation method and application of metabolism programming hydrogel, and the preparation method comprises the following steps: 1, oxidizing dextran Dex through sodium periodate NaIO4 to generate oxidized dextran ODex rich in aldehyde; 2, under the protection of nitrogen, carrying out amino dynamic Schiff base reaction of APTC and carrying out covalent binding with an aldehyde group of oxidized dextran ODex to form ODex-APTC; 3, mixing ODex-APTCC and CMC GOx solutions at room temperature to form hydrogel based on hemiacetal crosslinking and a hydrogen bond mechanism; according to the invention, autonomous'antibacterial repair 'dual-phase switching is realized through metabolic microenvironment reprogramming; in the stage of wound surface high-glucose infection, the hydrogel utilizes a diabetes wound surface high-glucose environment as an endogenous fuel, and glucose reduction, active oxygen mediated sterilization and microenvironment acidification are realized at the same time through reaction of glucose oxidase and glucose; when glucose oxidase reacts, the acid environment triggers a controlled H2S release program, and wound healing is accelerated by relieving oxidative stress and activating a regeneration signal channel.

The invention discloses a functionalized microarray pore plate, a preparation method thereof and application of the functionalized microarray pore plate in membrane protein ligand screening, and belongs to the technical field of biological medicine analysis. Amino and vinyl sulfuryl are covalently modified on the surface of the microarray pore plate to introduce an active site which can be specifically covalently bound with a membrane protein or a tag group, so that directional fixation of a target membrane protein on the surface of the microarray pore plate is realized, and the functionalized microarray pore plate is obtained. A functional microarray pore plate and a specific fluorescent probe are combined to screen a membrane protein ligand, the specific fluorescent probe is combined with a receptor active site of a fixed protein, and a detectable fluorescence signal change is generated by utilizing a competitive replacement mechanism of the probe and a candidate compound at the active site; the method is used for in-vitro screening and discovery of potential membrane protein ligands.

The invention discloses a method for detecting active watermelon bacterial fruit blotch bacteria by a PMA-PCR (Polymerase Chain Reaction) technology, which comprises the following steps: (1) collection and suspension of bacteria in a sample to be detected: suspending the sample with normal saline to obtain a bacterial suspension; (2) PMA treatment: adding propyl azide bromide (PMA) into the bacterial suspension; (3) blue light crosslinking: irradiating the bacterial suspension treated by the PMA under blue light to activate the covalent binding of the PMA and dead bacteria DNA (Deoxyribose Nucleic Acid); (4) DNA extraction: centrifugally collecting thalli, and extracting DNA by adopting a kit method; (5) PCR amplification: carrying out amplification by using a specific primer pair; and (6) result judgment: detecting an amplification product through agarose gel electrophoresis, wherein a target strip appears in an active bacterium sample, and no strip exists in a dead bacterium sample. The invention establishes a rapid, convenient, accurate and efficient method for detecting the active watermelon bacterial fruit blotch, so as to improve the disease detection capability and management efficiency.

A process for synthesizing and separating secretory IgA from a mixture of IgA monomer and IgA dimer is provided The process includes covalently binding affinity tagged or epitope tagged recombinant secretory component to the IgA dimer in the mixture and then binding the affinity tagged or an epitope tagged secretory IgA to immobilized moieties on the solid phase support resin to which the affinity tag or epitope tag binds and then eluting the affinity tagged or an epitope tagged secretory IgA with release buffer. A process for synthesizing and separating secretory IgM from a mixture of IgM and other plasma proteins is provided. A process is provided for inhibiting or preventing symptoms of necrotizing enterocolitis in a subject that includes the oral administration to the subject of a human polyclonal secretory IgA formed by the conjugation of human recombinant secretory component and pooled human plasma derived dimeric and polymeric.

The invention relates to a method for detecting at least one target nucleic acid sequence by means of a method for detecting at least one target nucleic acid sequence, comprising the steps of: i. Providing at least one target sequence-unspecific modular reporter complex comprising at least one label and at least two oligonucleotides, namely, i. a base strand comprising, 1. at least one mediator binding site, 2. at least one signal initiation oligo binding site, ii. at least one signal initiation oligo, wherein optionally the signal initiation oligo binding site of the base strand and the at least one signal initiation oligo are hybridized to each other but not covalently bonded and together form a signal complex, j. Providing at least one mediator probe, wherein the mediator probe comprises an oligonucleotide having at least one probe sequence and at least one mediator sequence, wherein the at least one probe sequence exhibits an affinity for at least one target nucleic acid sequence, and the at least one mediator sequence exhibits an affinity for at least one mediator binding site on the base strand of the at least one target sequence-unspecific modular reporter complex, k. PCR amplification of at least one nucleic acid sequence, I. Binding of a probe sequence of at least one mediator probe to at least one target nucleic acid sequence, m. Cleavage of the probe sequence of the at least one mediator probe bound to the at least one target nucleic acid sequence by a PCR polymerase, wherein the mediator sequence is released, n. Binding of at least one released mediator sequence to a mediator binding site of the at least one target sequence-unspecific modular reporter complex, o. Extension of the sequence of at least one mediator sequence bound to a mediator binding site by a PCR polymerase, wherein the bond is broken or prevented by hybridization of the at least one signal initiation oligo binding site and the at least one signal initiation oligo, thereby initiating a signal change, p. Detection of at least one signal change as evidence of the at least one target nucleic acid sequence.

The invention relates to a structure comprising a first material, a second material and a coupling agent capable of coupling the first material and the second material, the coupling agent comprises a moiety of formula (I) having a first nitrogen atom N'and a second nitrogen atom N '' (formula (I)) wherein A comprises a chain of 2 to 4 carbon atoms wherein the chain comprises a saturated linear chain, or wherein at least 2 carbon atoms in the chain are part of a non-aromatic cyclic structure; wherein the first nitrogen N'has a substituent L capable of covalently binding to the first material, and wherein the moiety of formula (I) forms a covalent dynamic bond with the second material via the second nitrogen N ''. Aspects of the invention also relate to a method of providing such a structure. # imgabs0 # (I).

A nanoparticle complex comprises a bifunctional conjugate capable of non-covalently binding to lipid nanoparticles in the nanoparticle complex.

Provided herein is a particle made of a protein covalently bound to a prebiotic carbohydrate, thereby forming a conjugate, wherein at least two said conjugates are covalently crosslinked via their carbohydrate units (e.g. by a phospho-di-ester bond). The said particle may be used for selectively promoting probiotic bacteria growth in the colon and may be used to deliver additional probiotic growth factors, or other bioactives and drugs to the colon. Furthermore, provided herein are methods for preparing the particle, and for delivering a substance bound to, or entrapped within the particle, into the gastrointestinal tract of a subject in need thereof.

The present invention discloses a collagen matrix flap covalently bound to fibroblast growth factor and a preparation method thereof. The collagen matrix flap is prepared by cross-linking oxidized sodium alginate (OSA) and bovine type I collagen. Subsequently, polyamide-amine dendrimer (PAMAM) is used as a connection carrier to covalently graft basic fibroblast growth factor (bFGF), thereby constructing a scaffold system capable of long-term sustained release of growth factors. The scaffold has good biocompatibility. Experiments have shown that the scaffold of the present invention exhibits clinical operability, superior biological activity to other growth factor-loaded scaffolds, and a more sustained growth factor release effect.

To provide a base preferable for covalently bonding at least one biologically active molecule to a carrier molecule.SOLUTION: Provided is a base which consists of a) polymer or oligomer structure, and b) at least one saponin SO1861 covalently bonded to the polymer or an oligomer structure, the base further contains c) a first chemical group for coupling the base to a carrier molecule in a covalently bonded state, e) the base is preferable for covalently bonding the at least one saponin SO1861 to the carrier molecule, and f) the carrier molecule contains or consists of any one of protein molecule, protein, peptide, nucleic acid, oligonucleotide, lipid, fat, fatty acid, nanoparticle and carbohydrate.SELECTED DRAWING: None

This invention discloses a type of click-type covalent drug, its regionally selective delivery system, and its applications. The invention comprises two parts: a click-type covalent drug and a tumor regionally selective delivery system. The click-type covalent drug consists of three parts: an azacyclic alkyne (DBCO), a small molecule drug, and a linker, having the structure of Formula 1. Optionally, the small molecule drug is a membrane protein inhibitor, an immunomodulator, or a chemotherapeutic drug. The click-type covalent drug reacts with azide-labeled target cells via a click reaction, forming a covalent bond that binds to the cell membrane, increasing the local drug concentration and prolonging the drug retention time, thereby enhancing selective inhibition of the target cells. The regionally selective delivery system is co-assembled from an amphiphilic block polymer and the click-type covalent drug for regionally selective delivery. This invention achieves covalent binding to azide-labeled tumor cells through the click-type covalent drug, while having no effect on unlabeled normal cells, reducing toxic side effects on normal tissues.

The invention provides a double-layer high-swelling hydrogel microneedle as well as a preparation method and application thereof, and belongs to the technical field of hydrogel microneedle materials. According to the invention, the N, N '-methylene bisacrylamide and polyethylene glycol dimethacrylate double cross-linking agents are utilized to improve the cross-linking efficiency and network uniformity of the gel and enhance the rigidity and toughness of the microneedle; the medicine Col is only loaded on the first layer of the needle tip, so that the microneedle does not cause inflammatory stimulation to the skin, and the utilization rate of the medicine on the needle tip is improved; a GO.Apt compound in the second-layer structure of the needle tip is used as a sensor, so that the microneedle can be used for uric acid marker sampling and specific detection; the carboxylated graphene oxide GO can be used as a quenching group of a sensor GO.Apt compound, and the mechanical property of the microneedle is enhanced; the covalent binding of the Apt. And the GO avoids the release of the Apt. And ensures the performance of the sensor; the microneedle provided by the invention has good morphology, can be effectively inserted into skin, and realizes release of drugs and extraction of skin interstitial fluid.

The present invention relates to polypeptides which are covalently bound to aromatic molecular scaffolds such that two or more peptide loops are subtended between attachment points to the scaffold. In particular, the invention describes peptides which are high affinity binders of PD-L1. The invention also includes drug conjugates comprising said peptides, conjugated to one or more effector and / or functional groups, to pharmaceutical compositions comprising said peptide ligands and drug conjugates and to the use of said peptide ligands and drug conjugates in preventing, suppressing or treating a disease or disorder mediated by PD-L1.

This invention discloses a method for detecting adiponectin in human serum using a polydopamine-modified wire mesh carbon electrode, specifically relating to the field of adiponectin detection technology. The method involves preparing a 2 mg / mL dopamine hydrochloride solution using a 10 mM tris(hydroxymethyl)aminomethane hydrochloride buffer solution (pH=8.5) and adding it dropwise onto the surface of the working electrode. A polydopamine-modified layer is formed by electropolymerization within a range of -800 mV to 600 mV using cyclic voltammetry. A conductive layer of a poly(3,4-ethylenedioxythiophene)-polystyrene sulfonic acid complex is then coated. Adiponectin antibodies are then immobilized on the surface of the modified layer via covalent binding of amino and quinone groups. After specific binding with a serum sample, the oxidation peak current change is read out from -100 mV to 350 mV in a buffer detection medium containing 5 mM ferricyanide redox pairs, and the concentration is output from a standard curve. This method improves the density of antibody immobilization sites and inhibits non-specific serum adsorption, enhancing signal stability and sensitivity, and enabling rapid, low-cost quantitative detection of trace amounts of serum.