3415results about "Nanomedicine" patented technology

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

The invention discloses an hMPV virus fusion protein F variant, an mRNA vaccine and application of the hMPV virus fusion protein F variant and the mRNA vaccine. The hMPV fusion protein F variant has amino acid residue difference on one or more sites relative to an hMPV fusion protein F with the login number of AGJ74096.1 in a GeneBank database, wherein the amino acid sequence of the hMPV fusion protein F variant is shown as SEQ ID NO: 1 or SEQ ID NO: 2. The mRNA vaccine designed based on the hMPV fusion protein F variant can induce a strong specific antibody reaction, the durability of the antibody in vivo is strong, the induced antibody can well neutralize an hMPV strain, the amplification of the hMPV in the lung is controlled, and the immune response of the hMPV in the lung is inhibited. Therefore, the specific antibody induced by the mRNA vaccine provides an effective basis for preventing hMPV virus infection related diseases and developing multi-combined vaccines.

The invention provides an immunologic adjuvant composition as well as a preparation method and application thereof. The immunologic adjuvant composition comprises an immunologic active agent and a liposome for loading the immunologic active agent, wherein the immunologic active agent comprises saponin and CpG oligodeoxynucleotide. The immunologic adjuvant composition can induce immune response of mammals to human herpes virus and / or hepatitis B virus, and can induce generation of a relatively high cellular immune response level. The QS21 and the CpG oligodeoxynucleotide are simultaneously loaded by using the liposome, so that the QS21 and the CpG oligodeoxynucleotide have a better synergistic effect in the aspect of promoting cellular immunity, and compared with a Shingrix commercially available vaccine, the vaccine can induce generation of a higher cellular immune response level.

The invention discloses a metal-doped carbon quantum dot as well as preparation and application of the metal-doped carbon quantum dot in a nano antibacterial agent, citric acid, urea and tea polyphenol are taken as precursors, metal salt is added, carbon dot synthesis and metal ion doping are synchronously completed in one step through a hydrothermal method, and the metal-doped carbon quantum dot is prepared; the surfaces of the metal-doped carbon quantum dots are negatively charged, and the metal-doped carbon quantum dots have peroxidase-like activity and catalase-like activity at the same time; the metal ions adopt any one of Fe < 3 + >, Cu < 2 + > and Ce < 3 + >; the metal-doped carbon quantum dots are in a sphere-like shape. The metal-doped carbon quantum dots prepared by the preparation method disclosed by the invention have peroxidase-like activity and catalase-like activity at the same time through precise doping of metal ions, and the two activities have a synergistic effect in an oral periodontitis microenvironment, so that a good antibacterial effect is achieved.

The invention provides a nitrogen-doped biomass-based fluorescent carbon quantum dot, a preparation method and application, and relates to the technical field of carbon nanomaterials, the method comprises the following steps: mixing a biomass material and deionized water under an ultrasonic condition, adding a nitrogen source, and carrying out ultrasonic dispersion to obtain a uniformly dispersed to-be-reacted solution; reacting the to-be-reacted solution at a preset temperature for a preset time to obtain a mixed product solution; and filtering and dialyzing the mixed product solution to obtain the nitrogen-doped biomass-based fluorescent carbon quantum dot solution. The nitrogen-doped biomass-based fluorescent carbon quantum dot prepared by the method shows a stable fluorescent effect and good water solubility, the free radical clearance rate can reach 90% or above, and the antibacterial rate can reach 99% or above. The method can be used in the fields of drug sustained release, bioimaging and the like.

The invention relates to mRNA comprising lipid nanoparticles and their medical uses. The lipid nanoparticles of the present invention comprise a cationic lipid according to formula (I), (II) or (III) and / or a PEG lipid according to formula (IV), as well as an mRNA compound comprising an mRNA sequence encoding an antigenic peptide or protein. The invention further relates to the use of said lipid nanoparticles as vaccines or medicaments, in particular with respect to influenza or rabies vaccination.

The invention discloses a nano-drug preparation system and a nano-drug preparation process. The nano-drug preparation system comprises a micro-fluidic chip, at least two feeding devices and a controller, the two feeding devices are communicated with the micro-fluidic chip and are respectively used for conveying different raw material liquids; each feeding device comprises a liquid path reversing coordination integrated block and two sucking and pushing mechanisms; the liquid path reversing coordination integrated block is provided with an input end and an output end; the two sucking and pushing mechanisms are both communicated with a fluid channel of the liquid path reversing coordination integrated block, each sucking and pushing mechanism achieves input or output of raw material liquid in a sucking or pushing mode, the driving ends of the two sucking and pushing mechanisms are both in signal connection with the controller, and the controller is used for controlling the two sucking and pushing mechanisms to alternately operate in the reverse direction; a one-way valve assembly is arranged in the liquid path reversing coordination integrated block. By means of the arrangement, the function of stably and continuously conveying raw material liquid without liquid mixing is achieved, the controllability of the preparation process of nano-drugs in the micro-fluidic chip is guaranteed, and the purpose of improving the preparation effect is achieved.

The present invention relates to pro-nano-emulsion compositions comprising at least one type of Omega 3 fatty acid capable of converting to nano-emulsion composition having average droplet size of below Ip, typically below 0.5p, and use thereof, particularly as dietary supplement, for preferential delivery of the Omega 3 fatty acid to heart and / or liver tissue of a subject consuming the composition, thereby maintaining normal cardiovascular / hepatic function and / or improving cardiovascular / hepatic disease or disorders.

This invention provides a transdermal nanoparticle delivery system for skin anti-inflammation, comprising a protein-blue calyx methyl ether-polymer shell capsule composite structure. The protein has an affinity for the skin, blue calyx methyl ether is loaded within the protein, and the polymer layer coats the surface of the protein. The blue calyx methyl ether nanoparticle transdermal delivery system has a size of 30-80 nm, and the polymer shell thickness is 10-35 nm. The surface properties of the polymer shell can be controlled according to the required transdermal delivery depth, enabling the protein to penetrate the skin barrier and be efficiently delivered deep into the skin, where the blue calyx methyl ether is then slowly released, targeting and alleviating skin inflammation. This addresses the drawback of low therapeutic efficacy of blue calyx methyl ether in treating skin inflammation due to unsatisfactory transdermal effects.

The invention relates to a two-dimensional nano heterojunction with space-time antibacterial and anti-inflammatory functions and a preparation method and application thereof, and belongs to the field of biomedical materials. The preparation method comprises the following steps: firstly, generating a BiCuSeO nanosheet, and resuspending the BiCuSeO nanosheet by using deionized water; the preparation method comprises the following steps: adding cerium nitrate hexahydrate into ethylene glycol, mixing with a BiCuSeO nanosheet resuspended by deionized water, and preparing the two-dimensional nano heterojunction with space-time antibacterial and anti-inflammatory functions by taking the BiCuSeO nanosheet as a seed and the cerium nitrate hexahydrate as a raw material through a hydrothermal synthesis method. Compared with the prior art, good photo-thermal performance of the heterojunction can be utilized, near infrared is used as an external field excitation source to regulate and control the temperature difference and excite the thermoelectric performance of the heterojunction, collaborative treatment of the photo-thermal performance and the thermoelectric performance is achieved, the enhanced anti-inflammatory function of the heterojunction is combined, and the heterojunction becomes a potential application material for infectious bone defects. The preparation method is simple, stable in condition and high in repeatability.

Microparticles for detecting biological materials are provided. Each of the microparticles includes: a core-shell structured microparticle consisting of a core including a magnetically responsive metal and a shell layer surrounding the core and having a uniform thickness; and capture probes introduced onto the shell layer to capture biological materials.

The invention belongs to the field of nano preparations, and discloses a preparation method of nano microspheres coated with a nicotinamide mononucleotide shell membrane structure, which comprises the following steps: coating NMN in a pregel formed by chitosan and sodium tripolyphosphate, heating and stirring, adjusting the pH value to separate out chitosan-coated nano microspheres, drying to remove moisture on the surfaces of the nano microspheres, and drying to obtain the nano microspheres coated with the nicotinamide mononucleotide shell membrane structure. Then selecting a water phase and an oil phase in a proper proportion, preparing a chitosan / sodium alginate composite nano-microsphere with sodium alginate as an outer membrane by adopting an emulsion cross-linking method, and washing and drying to obtain the NMN nano-microsphere with a core-shell structure. According to the invention, NMN is coated in the nano-microsphere which is composed of two materials and has a nuclear membrane structure, so that the stability of NMN in a natural environment can be improved, hydrolysis of NMN by gastric acid and gastrointestinal tract enzyme in a drug delivery process can be prevented, the bioavailability of NMN is improved, and a drug sustained release effect is achieved.

The invention relates to an external composition for hair growth regulation and a preparation method thereof, and belongs to the technical field of cosmetics and medicines. The composition disclosed by the invention comprises nano mesoporous carbon for enhancing permeation, various surface functional modifications on the nano mesoporous carbon, minoxidil, protein fragments, polypeptides, oligopeptides, amino acids, dutasteride, bimatoprost, traditional Chinese medicine extracts, vitamins, caffeine, nucleosides, growth factors and various auxiliary functional components. Wherein the pore diameter of mesopores of the nano mesoporous carbon for enhancing permeation is 2-30 nm, the total pore volume of the mesopores is 1.5-3.5 cm < 3 > / g, and the particle size of the nano mesoporous carbon is 50-200 nm. The prepared nano mesoporous carbon is granular, controllable in size, stable in structure, free of irritation to skin, capable of keeping inert to various active ingredients in the composition, capable of enhancing absorption and permeation of other ingredients on the skin, wide in application range, capable of compounding various types of active ingredients and high in biological safety.

This disclosure provides nanoparticle compositions, and use thereof for isolating and measuring proteins, protein degrader action, and cells. The disclosed nanoparticles can be identified by barcodes that reveal the identity of and post translational modifications to the bound protein using image processing techniques described herein.

The invention relates to ultra-small prussian blue nano-particles (T-USPB-C) carrying tirofian, a preparation method of the nano-particles and application of the nano-particles in preparation of drugs for reducing MVO and MIRI. According to the preparation method, the T-USPB-C is prepared by three steps. The T-USPB-C provided by the invention can carry tirofiban, and has catalase and superoxide dismutase simulated nano-enzyme activity. Moreover, the size of the nano-scale drug is required to reach a nano-scale size, and the drug can be efficiently cleared through kidney excretion, so that the potential long-term toxicity problem is minimized. The ultra-small prussian blue nanoparticle carrying the tirofian has an excellent active oxygen scavenging capability. Microvascular perfusion can be rapidly improved through the antithrombotic effect, then the protection effect is continuously achieved through the anti-oxidation and anti-inflammatory mechanism, and microvascular injury and MIRI are effectively prevented.

The invention discloses an acid responsive nanoprobe as well as preparation and anti-tumor application thereof. The nanoprobe has a core-shell structure, takes a nanoparticle self-assembled by a polycation aggregation-induced emission (AIE) photosensitizer and functional nucleic acid as a core, and takes a hyaluronic acid modified metal polyphenol network as a shell. The nanoprobe utilizes the acid responsiveness and broad-spectrum absorption performance of the metal polyphenol network and the diagnosis and treatment advantages of the polycation AIE photosensitizer to realize the acid responsiveness'switch 'controllable diagnosis and treatment of the tumor nanoprobe. In a neutral environment, the fluorescence is weak, the generation of ROS is less, and the background noise signal of the nanoprobe and the toxicity of non-targeted tissues are reduced; in an acid environment, fluorescence is enhanced, the ROS yield is improved, and the treatment efficiency and the curative effect monitoring accuracy are improved. The nano diagnosis and treatment probe is ingenious in design and simple to prepare, and can realize PDT and gene therapy synergistic anti-tumor, in-situ real-time report of early tumor treatment effect and assistance in precise cancer treatment.

Described herein are compositions, systems, and methods for targeted and controlled drug release. In some embodiments, the compositions, systems, and methods may comprise magnetoelectric silica nanoparticles for targeted and controlled release of chemotherapeutic drugs for cancer treatment. In some embodiments, an external magnetic field may be used to release one or more drugs from the magnetoelectric silica nanoparticles. The disclosed compositions, systems, and methods may improve drug targeting and reduce systemic drug toxicity.

The invention relates to the technical field of luminescent materials, and discloses an aggregation-induced emission material, nanoparticles as well as a preparation method and application thereof, the structural general formula of the aggregation-induced emission material is as follows: # imgabs0 #, R is selected from one of the following structures: # imgabs1 # aggregation-induced emission material takes a pyridine group as an acceptor unit, and the acceptor unit is selected from one of the following structures: 1 # imgabs2 # aggregation-induced emission material; a carbon-carbon double bond and a thiophene unit are used as pi bridges, and diphenylamine, bis (4-ethylphenyl) amine and bis (4-(tert-butyl) phenyl) amine are used as strong electron donors. The donors not only provide strong electron supply ability, but also become efficient rotors due to highly twisted molecular conformation, and endow the aggregation-induced emission material with long emission wavelength and aggregation-induced emission activity.

Certain embodiments of the invention provide a cationic lipid of formula (I):wherein R1, R2, R3 and R4 are defined as described herein, as well as methods of making these lipids. Certain embodiments of the invention also provide nucleic acid-lipid particles comprising a cationic lipid of formula (I), methods of making the lipid particles, and methods of delivering and / or administering the lipid particles.

The invention provides a nano-drug based on atomic layer deposition and a preparation method of the nano-drug, mesoporous silica is taken as a carrier, bimetallic nanoparticles are precisely deposited through ALD, and chemotherapeutic drugs and antibacterial components are loaded by combining amination and targeted PEG modification, so that the following functions are realized: (1) H2O2 in a tumor microenvironment is catalyzed to generate reactive oxygen species (ROS); removing fusobacterium nucleatum and damaging a biological membrane of the fusobacterium nucleatum; (2) GSH is consumed through Fenton reaction, and tumor drug resistance is reversed; and (3) the drug is released in pH response, and tumor cells are accurately killed. The invention further provides application of the nano-drug based on atomic layer deposition in treatment of related drug-resistant colorectal cancer induced by fusobacterium nucleatum, the process is controllable, the biocompatibility is excellent, the treatment effect on the drug-resistant colorectal cancer is remarkably improved, and the nano-drug has wide clinical application prospects.

The invention discloses a preparation method of ocimum gratissimum carbon dots and application of the ocimum gratissimum carbon dots in antibacterial drugs and food preservation. The preparation method of the ocimum gratissimum carbon dots comprises the following steps: performing steam distillation on ocimum gratissimum leaves to remove essential oil, drying in the shade, and crushing; adding an alcohol solvent into the powder, extracting, filtering, concentrating and drying to obtain an alcohol extract; dispersing the alcohol extract in distilled water, and stirring and pyrolyzing on a heating table; and diluting the pyrolysis product with distilled water, centrifuging, taking supernate, filtering with a filter membrane, dialyzing, and freeze-drying to obtain the carbon dots. The obtained carbon dots have a carbon dot-vesicle coexisting structure, and the average particle size is 5.732 + / -0.5 nm. The prepared ocimum gratissimum carbon dots can be applied to preparation of antibacterial drugs, especially drugs for inhibiting the activity of bacillus cereus and listeria, and the field of food preservation, and have the advantages of being wide in raw material source, simple in preparation method, low in cost, environmentally friendly and the like.

The invention provides a separation and purification method of urokinase, and relates to the technical field of separation and extraction. The separation and purification method comprises the following steps: S1, regulating the pH value of male urine to 8.5, standing, and taking supernate; s2, mixing the supernate with an adsorption material, and eluting with an eluent to obtain a urokinase crude product solution; the adsorption material is a core-shell silica gel polymer, Fe3O4atSiO2-NH2 is taken as a core, and polystyrene is taken as a shell; the eluent is a sodium citrate buffer solution with the concentration of 0.5 to 0.15 M, acetonitrile with the concentration of 1 to 5 weight percent and EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 0.5 to 1.5 mM; and S3, carrying out anion exchange column chromatography on the urokinase crude product solution, eluting with an eluent, and precipitating to obtain the urokinase. The urokinase prepared by the method has the advantages of high polymer urokinase content, high recovery rate and high activity yield.

The invention discloses a mucosal epithelial cell targeted oral ROS response type nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The oral ROS response type nano-enzyme comprises Ce-CDs carbon dots and a betaine polymer, and the betaine polymer is coated on the surfaces of the Ce-CDs carbon dots to form nano-particles; the Ce-CCDs carbon dots are prepared by taking a metal cerium source and chlorogenic acid as raw materials through a pyrolysis method. Through structural innovation and function integration, the prepared nano-enzyme shows outstanding advantages in the aspects of catalytic activity, targeted delivery, collaborative treatment, industrial application and the like, a new technical scheme is provided for efficient and safe treatment of inflammatory bowel diseases, and the nano-enzyme has remarkable technical innovation and clinical application value.

The invention discloses a nano-zinc oxide antibacterial and mildew-proof agent as well as a preparation method and application thereof. The nano-zinc oxide antibacterial and mildew-proof agent comprises a three-layer core-shell structure consisting of an inner core, a middle layer and an outer shell layer, the inner core is a nano zinc oxide tetrapod, the middle layer comprises a hydrophobic layer formed by a quaternary ammonium salt antibacterial and mildew-proof agent, and the shell layer comprises a hydrophilic layer formed by chitosan. According to the nano-zinc oxide antibacterial and mildew-proof agent disclosed by the invention, through the exquisite design of a three-layer core-shell structure, the defect that a traditional nano-zinc oxide antibacterial agent quickly loses efficacy is overcome, the synergistic interaction and multi-stage long-acting slow release of the antibacterial and mildew-proof agent are realized, and the durability of the antibacterial and mildew-proof agent is improved; the nano-zinc oxide antibacterial and mildew-proof agent provides long-term, efficient and environment-friendly antibacterial and mildew-proof protection for the water-based art coating, and has important practical application value.

The invention discloses a phosphatidylcholine gold nano CT (Computed Tomography) probe as well as a preparation method and application thereof, and belongs to the technical field of medical materials. The preparation method comprises the following steps: step S1, preparing a phosphatidylcholine ligand NH2-MPC; step S2, synthesis of a gold nanocluster GS-Au25 is carried out; and S3, the GS-Au25 is modified with a PC ligand, and the PC-Au25 is prepared. The ultra-small gold nano CT probe PC-Au25 with an Au25 gold nano cluster as a core and phosphatidylcholine PC as a surface ligand is obtained by optimizing and screening the core size and the surface ligand property of ultra-small gold nano particles. Wherein the 2-methacryloyloxyethyl phosphorylcholine is used as a PC ligand for the first time to modify the surfaces of the ultra-small gold nanoparticles, so that the surface modification of the ultra-small gold nanoparticles is realized, and the ultra-small gold nanoparticles can be efficiently and freely filtered through the kidney. The CT probe can be applied to the determination of the glomerular filtration rate through a CT imaging or blood test method.

Pharmaceutical compositions of semaglutide or salts thereof for intranasal administration for the treatment of diabetes, obesity, non-alcoholic fatty liver disease (NAFLD) or neurodegenerative diseases. The method of delivering semaglutide through the intranasal route avoids repeated injections and improves systemic absorption of semaglutide as compared to oral pastilles.

The invention discloses an aggregation-induced emission near-infrared photothermal molecule as well as a preparation method and application of the aggregation-induced emission near-infrared photothermal molecule, and belongs to the technical field of biomedicine. The invention provides the preparation method of the aggregation-induced emission near-infrared photothermal molecule, the synthesis process is simple and efficient, and the obtained novel near-infrared photothermal molecule has better luminescence performance or photothermal performance of a near-infrared second region. Meanwhile, the near-infrared aggregation-induced emission protein nanoparticles provided by the invention are simple in preparation process and excellent in performance, and can effectively cross nasal mucosa and target an A beta plaque part, so that AD visualization and photo-thermal / hypoxia relief combined treatment are realized.

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The present invention relates to polydopamine co-polymer nanoparticles as defined in the application. The present invention also relates to processes for the preparation of these polydopamine co-polymer nanoparticles, to pharmaceutical compositions comprising them, and to their use in therapy.