2007results about "Nanomedicine" patented technology

The invention discloses a metal-doped carbon quantum dot as well as preparation and application of the metal-doped carbon quantum dot in a nano antibacterial agent, citric acid, urea and tea polyphenol are taken as precursors, metal salt is added, carbon dot synthesis and metal ion doping are synchronously completed in one step through a hydrothermal method, and the metal-doped carbon quantum dot is prepared; the surfaces of the metal-doped carbon quantum dots are negatively charged, and the metal-doped carbon quantum dots have peroxidase-like activity and catalase-like activity at the same time; the metal ions adopt any one of Fe < 3 + >, Cu < 2 + > and Ce < 3 + >; the metal-doped carbon quantum dots are in a sphere-like shape. The metal-doped carbon quantum dots prepared by the preparation method disclosed by the invention have peroxidase-like activity and catalase-like activity at the same time through precise doping of metal ions, and the two activities have a synergistic effect in an oral periodontitis microenvironment, so that a good antibacterial effect is achieved.

This invention provides a transdermal nanoparticle delivery system for skin anti-inflammation, comprising a protein-blue calyx methyl ether-polymer shell capsule composite structure. The protein has an affinity for the skin, blue calyx methyl ether is loaded within the protein, and the polymer layer coats the surface of the protein. The blue calyx methyl ether nanoparticle transdermal delivery system has a size of 30-80 nm, and the polymer shell thickness is 10-35 nm. The surface properties of the polymer shell can be controlled according to the required transdermal delivery depth, enabling the protein to penetrate the skin barrier and be efficiently delivered deep into the skin, where the blue calyx methyl ether is then slowly released, targeting and alleviating skin inflammation. This addresses the drawback of low therapeutic efficacy of blue calyx methyl ether in treating skin inflammation due to unsatisfactory transdermal effects.

Microparticles for detecting biological materials are provided. Each of the microparticles includes: a core-shell structured microparticle consisting of a core including a magnetically responsive metal and a shell layer surrounding the core and having a uniform thickness; and capture probes introduced onto the shell layer to capture biological materials.

This disclosure provides nanoparticle compositions, and use thereof for isolating and measuring proteins, protein degrader action, and cells. The disclosed nanoparticles can be identified by barcodes that reveal the identity of and post translational modifications to the bound protein using image processing techniques described herein.

The invention relates to ultra-small prussian blue nano-particles (T-USPB-C) carrying tirofian, a preparation method of the nano-particles and application of the nano-particles in preparation of drugs for reducing MVO and MIRI. According to the preparation method, the T-USPB-C is prepared by three steps. The T-USPB-C provided by the invention can carry tirofiban, and has catalase and superoxide dismutase simulated nano-enzyme activity. Moreover, the size of the nano-scale drug is required to reach a nano-scale size, and the drug can be efficiently cleared through kidney excretion, so that the potential long-term toxicity problem is minimized. The ultra-small prussian blue nanoparticle carrying the tirofian has an excellent active oxygen scavenging capability. Microvascular perfusion can be rapidly improved through the antithrombotic effect, then the protection effect is continuously achieved through the anti-oxidation and anti-inflammatory mechanism, and microvascular injury and MIRI are effectively prevented.

The invention provides a nano-drug based on atomic layer deposition and a preparation method of the nano-drug, mesoporous silica is taken as a carrier, bimetallic nanoparticles are precisely deposited through ALD, and chemotherapeutic drugs and antibacterial components are loaded by combining amination and targeted PEG modification, so that the following functions are realized: (1) H2O2 in a tumor microenvironment is catalyzed to generate reactive oxygen species (ROS); removing fusobacterium nucleatum and damaging a biological membrane of the fusobacterium nucleatum; (2) GSH is consumed through Fenton reaction, and tumor drug resistance is reversed; and (3) the drug is released in pH response, and tumor cells are accurately killed. The invention further provides application of the nano-drug based on atomic layer deposition in treatment of related drug-resistant colorectal cancer induced by fusobacterium nucleatum, the process is controllable, the biocompatibility is excellent, the treatment effect on the drug-resistant colorectal cancer is remarkably improved, and the nano-drug has wide clinical application prospects.

The invention provides a separation and purification method of urokinase, and relates to the technical field of separation and extraction. The separation and purification method comprises the following steps: S1, regulating the pH value of male urine to 8.5, standing, and taking supernate; s2, mixing the supernate with an adsorption material, and eluting with an eluent to obtain a urokinase crude product solution; the adsorption material is a core-shell silica gel polymer, Fe3O4atSiO2-NH2 is taken as a core, and polystyrene is taken as a shell; the eluent is a sodium citrate buffer solution with the concentration of 0.5 to 0.15 M, acetonitrile with the concentration of 1 to 5 weight percent and EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 0.5 to 1.5 mM; and S3, carrying out anion exchange column chromatography on the urokinase crude product solution, eluting with an eluent, and precipitating to obtain the urokinase. The urokinase prepared by the method has the advantages of high polymer urokinase content, high recovery rate and high activity yield.

The invention discloses a mucosal epithelial cell targeted oral ROS response type nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The oral ROS response type nano-enzyme comprises Ce-CDs carbon dots and a betaine polymer, and the betaine polymer is coated on the surfaces of the Ce-CDs carbon dots to form nano-particles; the Ce-CCDs carbon dots are prepared by taking a metal cerium source and chlorogenic acid as raw materials through a pyrolysis method. Through structural innovation and function integration, the prepared nano-enzyme shows outstanding advantages in the aspects of catalytic activity, targeted delivery, collaborative treatment, industrial application and the like, a new technical scheme is provided for efficient and safe treatment of inflammatory bowel diseases, and the nano-enzyme has remarkable technical innovation and clinical application value.

The invention discloses an aggregation-induced emission near-infrared photothermal molecule as well as a preparation method and application of the aggregation-induced emission near-infrared photothermal molecule, and belongs to the technical field of biomedicine. The invention provides the preparation method of the aggregation-induced emission near-infrared photothermal molecule, the synthesis process is simple and efficient, and the obtained novel near-infrared photothermal molecule has better luminescence performance or photothermal performance of a near-infrared second region. Meanwhile, the near-infrared aggregation-induced emission protein nanoparticles provided by the invention are simple in preparation process and excellent in performance, and can effectively cross nasal mucosa and target an A beta plaque part, so that AD visualization and photo-thermal / hypoxia relief combined treatment are realized.

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The present invention is directed to lipid nanoparticles using cationic cholesterol for topical delivery for nucleic acid delivery, and when administered locally, side effects caused by systemic drug delivery can be minimized and protein expression can be confined to the site of administration. In addition, the duration of protein expression at the site of administration can be increased, and thus the lipid nanoparticles can be useful in the technical field related to nucleic acid therapeutics.

The invention belongs to the field of biological medicine, and relates to an application of an NR1D1 inhibitor in preparation of a medicine for treating sepsis, the NR1D1 inhibitor comprises siRNA capable of specifically silencing an NR1D1 gene in a targeted manner, and can up-regulate BMAL1 and recover IGF2BP2-ATP6V1B2 / ATP6V0c axis, so that sepsis immunosuppression and secondary infection defense are remarkably improved.

The invention discloses extraction of polydeoxyribonucleotide as well as preparation and application of a PDRN-Ce (Polydeoxyribonucleotide-Ce) microsphere cluster. The extraction and purification method comprises the following steps: mixing in-vitro fish testis tissues with water, carrying out crushing treatment and homogenization treatment on an obtained premix, carrying out proteolysis and solid-liquid separation, and collecting supernate to obtain enzymatic hydrolysate; wherein protease used for proteolysis comprises neutral protease; carrying out alcohol precipitation on the enzymatic hydrolysate by adopting an alcohol solvent, and collecting a precipitate; and dissolving the precipitate in water, desalting and drying. According to the extraction method, the purity and the yield of the poly-deoxyribonucleotide are remarkably improved, the preparation method is low in cost and convenient to operate, the prepared poly-deoxyribonucleotide is stable in property, small in molecular weight and easy to absorb by skin, in order to further improve the bioavailability of the extracted poly-deoxyribonucleotide, the PDRN-Ce microsphere cluster is prepared, and the PDRN-Ce microsphere cluster can be used for preparing the poly-deoxyribonucleotide. Good application prospects are realized.

The invention belongs to the technical field of biological medicines, and discloses lipid nanoparticles for muscle in-situ delivery as well as a preparation method and application of the lipid nanoparticles. According to the invention, four components Ugi of aldehyde, isonitrile, amine and carboxylic acid are subjected to a reaction to synthesize the ionizable cationic lipid. The ionizable cation lipid formed by introducing an ionizable cation head group into an isonitrile component has muscle in-situ nucleic acid delivery performance. The unsaturation degree of a lipid hydrophobic tail chain, the number of methylene spacers of an ionizable cation head group and LNP formula composition and process are further optimized, muscle in-situ selective mRNA delivery performance is enhanced, off-target liver expression is avoided, safety can be improved, and different application requirements of mRNA vaccines, nucleic acid drugs, gene editing and the like can be met.

The invention discloses application of nano-iron in vascular endothelial cells, and belongs to the technical field of cell engineering and genetic engineering. According to the invention, PEI coated Fe3O4 is taken as a research object, the application of PEI coated Fe3O4 in vascular endothelial cells is researched by adopting a molecular and cell biology method, and regulation of PEI coated Fe3O4 on vascular endothelial regeneration of the vascular endothelial cells after myocardial infarction is verified from multiple levels and multiple angles. The invention can provide a new reference for the recovery of cardiac functions after myocardial infarction, and has good application value.

The invention discloses a preparation method of an aluminum hydroxide adjuvant capable of regulating and controlling pH in stages, and aims to solve the problems of non-uniform particle size, unstable adsorption and large inter-batch difference in the traditional process. According to the method, phosphate radical modified hydroxyapatite nanocrystal seeds are taken as an induction template, and three-stage accurate pH regulation and control are carried out: a pre-crystal seed stage activates crystal seed active sites, a main crystallization stage guides directional growth of aluminum hydroxide, and an aluminum phosphate coating layer is formed in a stabilization stage. Compared with a traditional process, the finished product yield is improved, energy consumption is reduced, and the method is suitable for vaccine industrial production.

The invention relates to the field of drug-resistant bacterium infection, and discloses a g-C3N4 / Bi2Se3 nano heterojunction and a preparation method thereof.The preparation method comprises the steps that firstly, melamine powder is heated and calcined at the constant temperature, after a tubular furnace is naturally cooled, spark plasma sintering is conducted, nitrogen vacancies are introduced at the same time, and a blocky high-defect g-C3N4 material is obtained; grinding into powder, adding deionized water for ultrasonic treatment to obtain a first suspension, performing centrifugal treatment, freeze-drying the supernatant, and adding into an ethylene glycol solvent to obtain a second suspension; eG, polyvinylpyrrolidone, Bi (NO3) 3.5 H2O and Na2SeO3 are mixed and stirred, and the second turbid liquid is added; the preparation method comprises the following steps: adding Bi2Se3 into a reaction kettle, heating while stirring, adding a hydroxylamine EG solution, cooling to room temperature after reaction, centrifuging, washing, and carrying out vacuum drying on a precipitate to obtain a g-C3N4 / Bi2Se3 nano heterojunction; the problems that in the prior art, the catalytic efficiency is low, a collaborative mechanism does not achieve structure-function integrated design, the performance of a key functional material is limited, and the structural stability and biological suitability of the material are insufficient are solved.

The invention belongs to the technical field of catalyst materials, and particularly relates to a catalyst material integrated with Au-Ir cascade catalytic sites as well as a preparation method and application of the catalyst material. The catalyst material comprises micron-sized urchin-like carbonaceous particles loaded with cascaded Au clusters and Ir monatomic materials, and the micron-sized urchin-like carbonaceous particles have glucose oxidase-like activity, oxidases-like activity, peroxidase-like activity and NADH oxidase-like activity, so that metabolic inhibition can be combined with reactive oxygen attack to eliminate stubborn dental caries related biological membranes.

The invention provides application of tea extracellular vesicles in preparation of drugs for relieving or treating allergic respiratory diseases, and belongs to the technical field of extracellular vesicles. The invention provides application of tea extracellular vesicles in preparation of drugs for relieving or treating allergic respiratory diseases. The tea extracellular vesicles are proved to be capable of remarkably improving related symptoms of allergic respiratory diseases including allergic rhinitis, have good anti-inflammatory and immunoregulation effects, and have the application potential of being used as natural active ingredients for preparing drugs or nasal delivery preparations for treating the allergic respiratory diseases. Compared with existing hormone drugs, the tea extracellular vesicles have the advantages of being natural in source, high in safety, low in side effect and the like; moreover, the extracellular vesicles have nanoscale particle sizes, good mucous membrane permeability and bioavailability, can improve the curative effect, and are suitable for long-term intervention.

The invention relates to polymer nanoparticle and DNA nanostructure delivery compositions for non-viral delivery, and methods therefor. More particularly, the invention relates to polymer nanoparticle delivery compositions, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, and DNA nanostructure delivery compositions, such as DNA origami compositions, for the delivery of more than one payload, or for the delivery of a nucleic acid construct payload of 3 kB or more, and methods therefor.

The invention relates to a diatomic iron-iron site nano-enzyme constructed by relieving oxidative stress and cartilage degeneration and used for targeted osteoarthritis treatment. According to the invention, a nitrogen-doped porous carbon anchored diatomic iron nano enzyme catalyst (Fe2-NCs) with Fe-Fe dimer coordination is developed by using a'subject-object 'strategy. The Fe2-NCs protect cartilage cells from oxidative stress induced apoptosis by modulating ROS and active nitrogen species (RNS) and promoting O2 release. In addition, Fe2-NCs restores mitochondrial function by inhibiting NOX4 expression, improving ATP production, and normalizing COXIV levels. In an in-vivo OA model, the Fe2-NCs can reduce the expression of a pro-inflammatory medium COX-2 through an NF-kappa B signal channel, inhibit the up-regulation of MMP-13 and delay the degradation of type II collagen. The invention provides a new theoretical framework and methodological approach for the treatment of osteoarthritis, and has important clinical and scientific significance.

The invention relates to the technical field of biological medicine, in particular to a diagnosis and treatment integrated brain-targeted magnetic particle imaging tracer and a preparation method and application thereof. The preparation method comprises the following steps: loading tetrahydrocurcumin into glucan by adopting a nano-particle self-assembly technology to form glucan nano-particles loaded with tetrahydrocurcumin, synthesizing Fe3O4 nano-particles by adopting a chemical precipitation method, and embedding the Fe3O4 nano-particles on the glucan nano-particles to obtain the diagnosis and treatment integrated brain-targeted magnetic particle imaging tracer agent. The nano-particles are marked as THC (at) Dex-Fe3O4 nano-particles. The THC-Dex-Fe3O4 nanoparticles play a role in treating the Alzheimer's disease during brain imaging diagnosis, and the technical problems that magnetic particle imaging cannot be applied to diagnosis of the Alzheimer's disease and the Alzheimer's disease is difficult to treat are solved.

The invention belongs to the technical field of functional nano materials, and particularly relates to a preparation method of a detachable engine type braking nano motor, a nano drug carrier is jointly composed of HMSN and CAT modified PNA, tetraethyl orthosilicate serves as a silicon source, and solid silicon dioxide balls are synthesized; on the basis, tetraethyl orthosilicate is used as a silicon source, CTAB is used as a pore-forming agent to synthesize mesoporous silica coated solid silica spheres; etching the core silicon dioxide ball through sodium carbonate to obtain a hollow mesoporous silicon dioxide ball; then, amino modification is carried out on the CAT modified PNA2 through 3-aminopropyltrimethoxysilane, PNA1 is connected with HMSN through an amido bond, and a double-chain structure is formed by the CAT modified PNA2 and PNA1 through complementary base pairing. According to the nano motor provided by the invention, the engine CAT of the nano motor can be detached by adding the unwinding chain PNA3, so that in-vivo deceleration and braking are realized.

Disclosed are compositions and methods useful for targeting and internalizing molecules into cells of interest and for penetration by molecules of tissues of interest. The compositions and methods are based on peptide sequences that are selectively internalized by a cell, penetrate tissue, or both. The disclosed internalization and tissue penetration is useful for delivering therapeutic and detectable agents to cells and tissues of interest.

The invention relates to a silicon-based nano material for targeted therapy of brucellosis as well as a preparation method and application of the silicon-based nano material. The invention relates to a preparation method of a silicon-based nano material for targeted therapy of brucellosis. The preparation method comprises the following steps: (1) preparing magnetic mesoporous silica; (2) aminating the magnetic mesoporous silica, and modifying the magnetic mesoporous silica with a responsive material and yeast beta-glucan to obtain modified magnetic mesoporous silica; and (3) carrying out antibiotic loading on the modified magnetic mesoporous silica. According to the silicon-based nano material for targeted therapy of brucellosis as well as the preparation method and the application of the silicon-based nano material, the surface of magnetic macroporous mesoporous silica is modified with yeast beta-glucan capable of targeting M cells and glutathione responsive molecules, and a drug delivery system sensitive to a brucellosis environment is synthesized; the nano-carrier has targeted targeting selectivity for delivering and releasing drugs, the activity, slow release and target cell uptake efficiency of antibiotics are ensured, and the brucellosis treatment is more accurate and efficient.

The invention relates to the technical field of biomedical materials, in particular to an alendronate-modified ZIF-8 encapsulated TPP-carbon dot bone targeting nano-material and a preparation method and application thereof.The alendronate-modified ZIF-8 serves as a carrier, TPP-modified carbon dots are encapsulated in the carrier, citric acid, urea and cerium salt serve as raw materials of the TPP-modified carbon dots, and the alendronate-modified ZIF-8 encapsulated TPP-carbon dot bone targeting nano-material is prepared through a one-step method. After cerium-doped carbon quantum dots are synthesized through a hydrothermal method, TPP is grafted through an amide method, alendronate endows the material with a bone targeting function, and TPP modified carbon dots endow the material with antioxidant and mitochondrial targeting functions. According to the invention, the bone targeting property is excellent, and bone focuses are efficiently enriched; the anti-oxidation and anti-inflammatory capacities are high, and the infected microenvironment can be improved; zIF-8 packaging is stable, and functional components can be controllably released; multiple components cooperate to promote bone repair, accumulation of non-target organs is little, and the composition is safe, reliable and suitable for treatment of various bone-related diseases.

The invention discloses iron oxide nanoparticles capable of responding to particle size change in a tumor microenvironment and a preparation method of the iron oxide nanoparticles, and belongs to the technical field of biomedical nano materials. The nano-particle comprises an iron oxide nano-crystal core with the particle size of 3-5nm and a tumor microenvironment response layer coating the surface of the iron oxide nano-crystal core. The response layer contains groups, such as disulfide bonds, enzyme digestion peptide fragments and the like, which can respond to acidic pH, high-concentration reduced glutathione or matrix metalloproteinase and other tumor characteristic stimuli to generate structural changes. In a tumor microenvironment, the hydration particle size of the nanoparticles can be directionally and controllably converted from 3-20 nm to 20-100 nm (or reversely). The preparation method mainly comprises two steps of preparing the core by a thermal decomposition method and modifying the surface response layer. The technical problems of insufficient tumor targeted enrichment capacity, limited imaging contrast and difficulty in balancing permeation and retention caused by fixed particle size of the existing iron oxide nanoparticles are solved, and the accuracy and efficiency of tumor diagnosis and treatment can be remarkably improved.

The invention discloses a core-shell nano-enzyme composite spray gel dressing and a preparation method and application thereof.The dressing comprises a component A prepared from sodium alginate, N-isopropylacrylamide PNIPAM, core-shell nano-enzyme and curcumin and a component B of a divalent metal salt solution, and the component A and the component B are sprayed at the same time to form the core-shell nano-enzyme composite spray gel dressing. The preparation method of the core-shell nano enzyme comprises the following steps: mixing potassium ferrocyanide, ferric trichloride and polyvinylpyrrolidone to obtain a Prussian blue nano particle precursor solution; carrying out ultrasonic emulsification treatment and replacement on the curcumin core solution and the Prussian blue nanoparticle precursor solution; and freeze-drying to obtain the core-shell nano-enzyme. Through rapid ionic crosslinking of sodium alginate and divalent metal ions and the temperature-sensitive phase change characteristic of the temperature-sensitive polymer poly (N-isopropylacrylamide), the gel dressing is rapidly formed, the damaged skin is effectively covered and protected, the excellent antioxidant, anti-inflammatory and antibacterial capabilities are achieved, and the healing cycle of the radiodermatitis wound can be remarkably shortened.