26 results about "Articular cartilage" patented technology

The application belongs to the technical field of biomedical materials, and discloses a collagen-based double-network porous hydrogel scaffold capable of recruiting endogenous stem cells and a construction method and application thereof. The scaffold takes collagen II (Col-II) and oxidized methyl acrylated hyaluronic acid (AHAMA) as an organic phase, and is constructed into a physical / chemical double-network structure through Schiff base dynamic crosslinking and ultraviolet light-induced methacryl radical polymerization. An air-in-water emulsion template method is used to form a through-porous structure, and 5' aldehyde-modified nucleic acid aptamer Apt19S is anchored in the scaffold network to achieve specific recruitment of endogenous pluripotent stem cells. The scaffold has excellent mechanical properties, a biomimetic extracellular matrix microenvironment and a targeted stem cell recruitment function, can effectively promote the directional differentiation of stem cells into chondrocytes, avoids the immune rejection and ethical issues caused by exogenous stem cell transplantation, and is suitable for the field of articular cartilage defect repair.

The invention discloses a suture-containing anchor device for fixing articular cartilage stripping and a fixing method. The device comprises an anchor, a soft needle, a buckle cylinder and a suture; the anchor is made of titanium alloy absorbable materials and is provided with a double-spiral decompression groove and a suture channel. The suture is made of eight strands of ultra-high molecular weight polyethylene coated with polylactic acid, one end is fixed with the anchor, and the other end is welded with a nickel-titanium alloy soft needle through laser; the buckle cylinder is detachable and wraps the suture and the soft needle connecting section. The method comprises the following steps: resetting a bone block and temporarily fixing the bone block, drilling a central bone tunnel, implanting an anchor nail, installing a buckle cylinder, then enabling a soft needle with a suture to penetrate out, drilling a peripheral bone tunnel, threading into a tension band in a bundle splitting manner, adjusting the tension to 0.5-1.2 N / mm < 2 >, and then combining and locking the SMC sliding knot and the surgical knot. Minimally invasive fixation is achieved, mechanical stability and tension uniformity are improved, and cartilage healing is facilitated.

Provided is a highly safe articular cartilage regeneration material. This articular cartilage regeneration material contains: a scaffold-free artificial tissue in which synovial-derived mesenchymal stem cells cultured with a serum-free medium form a three-dimensional structure; and an extracellular matrix derived from mesenchymal stem cells. The articular cartilage regeneration material is implanted so as to be attached to a site of lost or damaged articular cartilage.

A formulation for use in treating osteoarthritis by modifying the shape change of bone(s) underlying articular cartilage is disclosed. The use comprises evaluating the bone shape of the patient's joint, injecting the patient with a peptide of SEQ ID No. 1 or applying other therapeutic interventions that can reduce the shape change of the bone(s) underlying articular cartilage, and thereafter evaluating the bone shape of the patient's joint.

The present invention relates to novel long non-coding RNAs (lncRNA) involved in the regulation of hypertrophy of chondrocytes, wherein RUNX2 was regulated at the post-translational level during hypertrophic differentiation of the chondrocyte cell line TC28a2 and two novel RUNX2-binding lncRNAs among numerous lncRNAs were identified, leading to the present invention. It is expected that bone-related diseases can be diagnosed by measuring the expression of the identified specific lncRNAs, and furthermore, the vascularization or calcification of articular cartilage can be effectively suppressed by inhibiting the hypertrophy of chondrocytes through inhibiting the expression of the identified specific lncRNAs, which is expected to be highly effective in the prevention or treatment of bone-related diseases.

The application provides a compound preparation for improving osteoporosis and osteoarthritis, a preparation method and application thereof, and belongs to the technical field of biological medicines. The compound preparation contains the following raw materials: glutathione, curcumin, calcium carbonate, vitamin D3, hydrolyzed collagen, and glucosamine sulfate; wherein the glutathione exists in the form of a cyclodextrin inclusion compound. The preparation method comprises the following steps: mixing the calcium carbonate, the hydrolyzed collagen, the glucosamine sulfate and excipients, then adding an equal amount of a premix of the glutathione cyclodextrin inclusion compound, the curcumin and the vitamin D3 in a stepwise manner, and performing dry granulation and tabletting to obtain the compound preparation. The compound preparation can simultaneously increase bone density, protect articular cartilage, reduce the levels of oxidative stress and inflammation, and can be used for preparing medicines or health foods for preventing or adjuvant treating osteoporosis and osteoarthritis. The application has a scientific formula, a stable process and a good application prospect.

The invention discloses an articular cartilage MRI (Magnetic Resonance Imaging) image intelligent enhancement and contrast optimization system, which belongs to the technical field of medical image processing and comprises a cartilage feature extraction module, a self-adaptive enhancement module, a contrast optimization module and a quality evaluation feedback module, the cartilage feature extraction module realizes cartilage region classification and structural analysis based on T2 relaxation time distribution features and multi-scale structure tensor features; the adaptive enhancement module performs differential enhancement according to the region type and the structural feature; the contrast optimization module realizes contrast dynamic optimization through nonlinear mapping and edge gradient adaptive adjustment; according to the articular cartilage MRI image display system and method, the display quality of the articular cartilage MRI image is remarkably improved, the cartilage display definition is improved by 73% or above compared with that of an original image, and a high-quality imaging basis is provided for accurate diagnosis of chondropathy.

A system and method for a ROSE-Reverdin / DARE osteotomy obviates severe deformities having a high intermetatarsal angle and which require closure via osteotomy (bone cut) of the first and / or fifth metatarsal in the midshaft or proximal aspect of the bone. The ROSE osteotomy is utilized to achieve this correction and is performed without need for fusion of the first metatarsal cuneiform joint. The ROSE osteotomy achieves correction of the intermetatarsal angle via rotation of the first and / or fifth metatarsal about a proximal based axis point. The Reverdin / DARE osteotomy is required to correct a deviated articular cartilage deformity at the head of the first metatarsal and / or additional slight correction of the IM angle. The Reverdin / DARE osteotomy achieves correction by wedging of the cut to realign the cartilage and translation of the head of the first metatarsal for further IM correction. A unique jig is used to achieve this cut and fixation.

An apparatus and methods are provided for a dermal cap for resurfacing a metatarsal head of the foot or a metacarpal head of the hand. The dermal cap comprises an outer surface having a convex shape, an inner surface having a concave shape, and a rounded peripheral surface joining the inner surface and the outer surface. The inner surface can be placed onto a bone head such that the outer surface provides a bone head surface resembling the original articular cartilage. The outer surface effectively restores the articular cartilage and thus relieves pain and facilitates better joint function. The dermal cap can be formed by stretching it over a rounded mandrel shaped similarly to the size and anatomy of the bone head to be treated. The dermal cap can be freeze-dried on the rounded mandrel to set the desired anatomy of the dermal cap.

This invention relates to a polyamino acid-based in-situ cartilage regeneration porous gel scaffold and its preparation method. Phenylboronic acid ester is esterified and linked with polyethylene glycol-modified Kartogenin to obtain phenylboronic acid ester-modified PEGKGN. The PEGKGN modified with phenylboronic acid ester is then grafted onto the side chains of polyamino acids with allyl polyethylene glycol to obtain a graft copolymer PLBPKA. The graft copolymer PLBPKA is blended with small molecules of Kartogenin and a silk fibroin solution to form a drug-loaded self-assembly network under shear force. Finally, the polyamino acid-based in-situ cartilage regeneration porous gel scaffold is constructed through ultraviolet light crosslinking and freeze-phase separation, which is a differentially released drug hydrogel scaffold. Compared with existing technologies, the scaffold of this invention has good biocompatibility and biocontrollable degradation, and can rapidly release drugs in inflammatory environments to promote stem cell migration; while in normal physiological environments, it slowly releases drugs to promote the differentiation of stem cells into cartilage. Therefore, it can promote in-situ regeneration of articular cartilage in the absence of exogenous stem cells.

The invention belongs to the field of biological medicine or health food, and particularly relates to a composition for improving bone joint health and application thereof. Through wide research, the combination of the chondroitin sulfate sodium with small molecular weight and N-acetylglucosamine achieves unexpected synergistic effects in the aspects of inhibiting inflammatory factor level, improving arthralgia / stiffness, reducing joint swelling, maintaining articular cartilage health and the like; and a foundation is laid for developing products capable of effectively maintaining the health of bone joints.

The invention discloses joint health-care soft sweets, and belongs to the field of health-care foods. Comprising hyaluronic acid or hyaluronate, a glucosamine compound without strongly acidic anions, a sugar body, a colloid, an additive and purified water. Compared with the prior art, firstly, glucosamine compounds, such as acetyl sugar or lemon glucosamine, which are milder in metabolism are selected as nutrient elements of the health-care soft sweets, so that the problem of stomach stimulation caused by introduction of sulfate radicals or chlorine radicals is fundamentally avoided, and damage to gastric mucosa can be reduced; secondly, the glucosamine and the hyaluronic acid are compounded according to a specific proportion, have a synergistic effect and can jointly protect articular cartilage and lubricate joints; and finally, through a specific material pretreatment method and process, the technical problems of high viscosity, moisture absorption, caking and the like caused by hyaluronic acid are solved, and the homogeneity, stability and industrial productivity of the composition in the soft sweets are ensured.

The invention discloses application of LRP1 in preparation of osteoarthritis medicines, and belongs to the technical field of biological medicines. The m6A methylase can regulate the immune homeostasis of macrophages and regulate chondrogenic differentiation of bone marrow mesenchymal stem cells through LRP1 mediated lipid metabolism reprogramming, so that repair and regeneration of articular cartilage are promoted, and a potential new immune metabolism dual regulation strategy is provided for treatment of osteoarthritis patients.

The invention belongs to the technical field of cartilage tissue engineering, and discloses a cartilage tissue engineering scaffold with a cross-scale fiber structure and a preparation method and application thereof. According to the method, a handheld electrospinning gun is used, and a coarse fiber grid layer and a non-woven fiber structure layer are sequentially formed on a defective cartilage model by controlling the distance between a gun head and the cartilage defect model; and soaking in a hydrogel solution, and curing to obtain the cartilage tissue engineering scaffold. According to the method provided by the invention, the cross-scale fiber structure scaffold can be rapidly prepared on the curved surface structure, and the scaffold can be matched with the articular cartilage defect boundary and curved surface characteristics; and moreover, the scaffold can be endowed with finer and smaller regular aperture, is beneficial to colonization of stem cells, and can be widely applied to repair of cartilage defects.

Disclosed herein are encapsulated microspheres comprising a plurality of cells, e.g., chondrocytes, where the plurality of cells is arranged into clusters. The disclosed encapsulated microspheres recapitulate many features of native articular cartilage and are able to be cultured on cartilage or bone to cure defects in the cartilage. Also disclosed herein are tissue constructs, orthopedic grafts, methods of generating the disclosed encapsulated microspheres, and methods of testing a candidate therapeutic.

This invention discloses a method for preparing a fusion protein of sialic acid-binding immunoglobulin-like lectin 6 extracellular fragment and human immunoglobulin G1-Fc fragment, and its application, belonging to the field of biomedical technology. The fusion protein consists of a functional fragment of the Siglec-6 extracellular region linked to the Fc fragment of human IgG1 via a peptide linker. Its amino acid sequence is selected from one of SEQ ID No. 1, SEQ ID No. 2, or SEQ ID No. 3, or a variant with more than 90% homology to the above sequences and retaining the biological activity of Siglec-6. The preparation method includes: cloning the Siglec-6 fragment and the coding sequence of the Fc fragment, constructing a recombinant expression plasmid and transfecting it into mammalian cells to induce expression, and purifying the target protein by affinity chromatography. The fusion protein vcSig6-Fc of this invention has extremely high structural stability and ligand binding affinity, can significantly inhibit the expression of inflammatory factors and matrix-degrading enzymes in chondrocytes, and shows excellent performance in promoting COL2A1 expression, thus exhibiting good protective effects on articular cartilage and therapeutic effects on rheumatoid arthritis.

This invention relates to a matrix stiffness-induced chondrogenic self-healing hydrogel, its preparation method, and its applications, belonging to the field of hydrogel preparation technology. The matrix stiffness-induced chondrogenic self-healing hydrogel of this invention utilizes biosafe and biocompatible natural polymer materials (sodium alginate, carboxymethyl chitosan, and silk fibroin). The network structure and matrix stiffness of the hydrogel are precisely controlled through intermolecular hydrogen bonds, dynamic covalent bonds, and a β-sheet synergistic strategy to prepare a matrix stiffness-induced chondrogenic self-healing hydrogel. The matrix stiffness-induced chondrogenic self-healing hydrogel prepared by this invention possesses the safety of a natural biomedical material, excellent self-healing properties, and the ability to induce stem cell chondrogenic differentiation and promote articular cartilage regeneration.

The present invention belongs to the field of biotechnology. Provided are a circular IL-10 mRNA nano-preparation, and a preparation method therefor and the use thereof. The circular IL-10 mRNA nano-preparation is prepared by means of the following method: 1, synthesizing a chemically modified circular IL-10 mRNA in vitro; and 2, preparing, by means of self-assembly, a circular IL-10 mRNA nano-preparation that can be delivered in vivo. The circular IL-10 mRNA inhibits injury of articular cartilage by means of regulating immune cells in the synovial membrane of an articular cavity, thereby achieving the effect of treating inflammatory joint diseases.

This invention relates to the field of organoid bioprinting technology, and discloses a method for preparing articular cartilage organoids, a bio-ink for encapsulating articular cartilage organoids, the preparation method thereon, and their applications. The preparation method includes: pretreating the raw material to be cultured in a basal culture medium containing a nanomedicine additive, wherein the nanomedicine additive chemically comprises mesoporous silica nanoparticles loaded with docetaxel and polyethyleneimine modified on the mesoporous silica nanoparticles; culturing the pretreated raw material in a cartilage organoid induction culture medium containing docetaxel; wherein the raw material to be cultured contains bone marrow mesenchymal stem cells. The articular cartilage organoids prepared by this method have a distinct cartilage-bone bio-interface, and the pretreatment process can help maintain the long-term phenotype of the cartilage organoids by inhibiting endochondral ossification during their development.

This invention discloses an inflammatory pretreatment xenogeneic synovial cell-derived vesicle, its preparation method, and its application in the treatment of osteoarthritis, belonging to the field of biomedical technology. The preparation method includes: obtaining and culturing rabbit synovial fibroblast-like cells; subjecting the cells to inflammatory pretreatment for 40-60 hours using a culture medium containing 70-80 ng / mL tumor necrosis factor-α (TNF-α); collecting the cell supernatant, and purifying it by differential centrifugation and ultracentrifugation to obtain the vesicles. The vesicles have a particle size distribution of 100-1000 nm, with a higher proportion of larger particles (>800 nm) than natural vesicles. This invention also provides a pharmaceutical composition containing these vesicles and their application in the preparation of drugs for treating osteoarthritis. Experiments show that these vesicles can significantly inhibit subchondral ossification and osteophyte formation, effectively protect articular cartilage, and have a therapeutic effect superior to natural vesicles, providing a novel cell-free therapeutic strategy for osteoarthritis.

This invention provides chondrocyte membrane-modified nano-mitochondrial hybrid vesicles, their preparation method, and applications. These vesicles are hybrid vesicles possessing both chondrocyte membrane and mitochondrial components, containing specific proteins of both mitochondria and chondrocyte membranes, and have a particle size of 50-250 nm. This invention provides chondrocyte membrane-modified nano-mitochondrial hybrid vesicles capable of targeting cartilage, improving chondrocyte mitochondrial function, and remodeling cartilage energy metabolism. They can directly replenish and restore the energy metabolism of damaged chondrocytes; possess homologous targeting derived from chondrocytes, can specifically hom to articular cartilage tissue, and be efficiently taken up by chondrocytes; the preparation method is stable and controllable, can be stored long-term, is suitable for standardized production, and can be applied to the prevention and treatment of osteoarthritis.

The invention discloses a preparation method of an articular cartilage three-dimensional nanofiber scaffold, and relates to the technical field of medical biological materials. The method comprises the following steps: dissolving a degradable polymer and a natural polymer material in a solvent to prepare a spinning solution; performing electrostatic spinning on the spinning solution to obtain a cartilage scaffold two-dimensional nanofiber membrane; performing foaming treatment on the cartilage scaffold two-dimensional nanofiber membrane, and converting the cartilage scaffold two-dimensional nanofiber membrane into a three-dimensional nanofiber cartilage scaffold; and soaking the freeze-dried three-dimensional nanofiber cartilage scaffold in a mixed solution of epigallocatechin gallate and SrCl2 for chemical modification, and freeze-drying the three-dimensional nanofiber cartilage scaffold to obtain a finished product of the three-dimensional nanofiber cartilage scaffold. The articular cartilage three-dimensional nanofiber cartilage scaffold prepared by the method can effectively simulate a three-dimensional ECM structure of natural cartilage, and cartilage regeneration is facilitated.

The utility model relates to the technical field of sample preservation devices, in particular to a portable articular cartilage tissue sample preservation device. Comprising a box body and a cover body hinged to the box body, and the box body and the cover body are connected through a plurality of lock catches; an inner box is arranged in the box body, an inner cover is arranged in the cover body, buffering cavities are formed between the box body and the inner box and between the cover body and the inner cover, four supporting columns are arranged in each buffering cavity, each buffering cavity is divided into four parts, and a first buffering assembly is arranged between every two adjacent supporting columns in each buffering cavity. A plurality of buffer components II are arranged between the bottom of the inner box and the bottom in the box body and between the top of the inner cover and the top in the cover body; a heat preservation frame is arranged in the inner box through a plurality of fixing columns, and a plurality of thermos bottles are arranged between the inner side of the inner box and the outer side of the heat preservation frame; a plurality of limiting grooves are formed in the bottom in the inner box, sample storage bottles are arranged in the limiting grooves, and an abutting assembly is arranged in the inner cover. The utility model provides a portable articular cartilage tissue sample preservation device which is sufficient in vibration relief and reasonable in thermos bottle layout.

The utility model discloses a detachable knee joint teaching model, which relates to the field of knee joint models and comprises a base, a knee joint model is fixedly mounted on the base and comprises a femoral condyle part and a tibial plateau part, and the outer sides of the femoral condyle part and the tibial plateau part are coated with the same simulation skin part. A patella part, an articular cartilage layer and a collateral ligament layer are arranged in the cavity, and the infrapatellar fat pad, the patella part, the articular cartilage layer and the collateral ligament layer are filled with liquid with different colors. According to the utility model, the components in the knee joint model are matched to form a simple training model, so that a user can judge whether an injection position is correct or not from liquid flowing out of the drain hole, and the device plays a role in assisting medical personnel to practice the injection technology; the internal components can be replaced by new components to facilitate the next use, and both teaching and practicability are considered.

Provided is a highly safe method for treating articular cartilage. This method for treating articular cartilage comprises a step for introducing a scaffold-free artificial tissue in which serum-free cultured synovial membrane-derived mesenchymal stem cells form a three-dimensional structure.

The bionic articular cartilage structure energy absorption headrest comprises an outer pillow and an inner pillow, the outer pillow is fixedly installed on the inner pillow in a sleeved mode, an installation rod is fixedly arranged at the bottom of the inner pillow, the bottom end of the installation rod penetrates through the outer pillow and extends downwards, the inner pillow comprises a first inner pillow base layer, and the first inner pillow base layer is made of stainless steel materials. The outer pillow comprises a buffering energy-absorbing layer, a first pillow cushion layer is fixedly arranged on the outer side of the buffering energy-absorbing layer, a waterproof layer is fixedly arranged on the outer side of the first pillow cushion layer, a hollow groove is formed in the buffering energy-absorbing layer, a plurality of buffering plates and buffering springs are fixedly arranged in the hollow groove, and the buffering plates and the buffering springs are arranged at intervals. The automobile seat headrest solves the problems that most of traditional automobile seat headrests adopt simple filler structures, the energy absorption effect is limited, impact force generated during collision is difficult to effectively buffer, in addition, an existing headrest structure is insufficient in stability and prone to deformation or damage in the collision process, and the protection performance of the existing headrest structure is further reduced.