41 results about "Articular cartilage" patented technology

To provide a compound and the use thereof for the treatment for regeneration of connective tissue following the degeneration process of articular cartilage due to osteoarthritis, in the case of humans, the degeneration process of articular cartilage caused by excessive stress, for the treatment of skin aging, and for the prevention of skin cancer.SOLUTION: For example, an amino acid derivative of glucosamine having the following structure is provided.SELECTED DRAWING: None

The application belongs to the technical field of biomedical materials, and discloses a collagen-based double-network porous hydrogel scaffold capable of recruiting endogenous stem cells and a construction method and application thereof. The scaffold takes collagen II (Col-II) and oxidized methyl acrylated hyaluronic acid (AHAMA) as an organic phase, and is constructed into a physical / chemical double-network structure through Schiff base dynamic crosslinking and ultraviolet light-induced methacryl radical polymerization. An air-in-water emulsion template method is used to form a through-porous structure, and 5' aldehyde-modified nucleic acid aptamer Apt19S is anchored in the scaffold network to achieve specific recruitment of endogenous pluripotent stem cells. The scaffold has excellent mechanical properties, a biomimetic extracellular matrix microenvironment and a targeted stem cell recruitment function, can effectively promote the directional differentiation of stem cells into chondrocytes, avoids the immune rejection and ethical issues caused by exogenous stem cell transplantation, and is suitable for the field of articular cartilage defect repair.

The present invention relates to the technical field of joint repair hydrogels, and in particular to a three-dimensional biohydrogel and its preparation method and application. The three-dimensional biohydrogel, by weight, comprises: 1-5 parts of methacrylic anhydride hyaluronic acid HAMA, 0.05-0.15 parts of initiator, 3-8 parts of hydrogenated soybean phosphatidylcholine liposomes and 7-13 parts of stem cell solution; the preparation method of the stem cell solution is: gelatin and water are mixed to obtain a gelatin solution; stirring at 40-60 ° C to prepare a dispersed phase; adding an emulsifier to the oil to prepare a continuous phase; the dispersed phase and the continuous phase are prepared by microfluidic technology to obtain uniform droplets, and the droplets are freeze-dried to obtain gelatin microspheres; stem cells are added to the gelatin microspheres and incubated at 25-37 ° C for 2-4 hours to obtain a stem cell solution. The present invention has long-lasting efficacy and significant safety, and can fundamentally repair articular cartilage.

The invention discloses a suture-containing anchor device for fixing articular cartilage stripping and a fixing method. The device comprises an anchor, a soft needle, a buckle cylinder and a suture; the anchor is made of titanium alloy absorbable materials and is provided with a double-spiral decompression groove and a suture channel. The suture is made of eight strands of ultra-high molecular weight polyethylene coated with polylactic acid, one end is fixed with the anchor, and the other end is welded with a nickel-titanium alloy soft needle through laser; the buckle cylinder is detachable and wraps the suture and the soft needle connecting section. The method comprises the following steps: resetting a bone block and temporarily fixing the bone block, drilling a central bone tunnel, implanting an anchor nail, installing a buckle cylinder, then enabling a soft needle with a suture to penetrate out, drilling a peripheral bone tunnel, threading into a tension band in a bundle splitting manner, adjusting the tension to 0.5-1.2 N / mm < 2 >, and then combining and locking the SMC sliding knot and the surgical knot. Minimally invasive fixation is achieved, mechanical stability and tension uniformity are improved, and cartilage healing is facilitated.

The invention discloses a joint health-care tablet based on glucosamine hydrochloride and sodium chondroitin sulfate and a preparation method, and relates to the technical field of medical health care. The joint health-care tablet comprises the following components: glucosamine hydrochloride, sodium chondroitin sulfate, a frankincense extract, an aesculus chinensis extract, microcrystalline cellulose, povidone, croscarmellose sodium and silicon dioxide. Through scientific proportioning of various active components such as the glucosamine hydrochloride, the chondroitin sulfate sodium, the frankincense extract and the aesculus chinensis extract, comprehensive protection of joint health is realized, the glucosamine hydrochloride and the chondroitin sulfate sodium are used as basic components, repair and regeneration of articular cartilage are promoted, and the joint health is improved. Boswellic acid compounds in the frankincense extract and aescin in the aesculus chinensis extract have anti-inflammatory and analgesic effects and can effectively relieve arthralgia and inflammation, multiple effects are synergistic, and the joint health care effect is improved.

This invention provides methods for optimal implantation of a solid substrate for promoting cell or tissue growth or restored function in an osteochondral, bone or cartilage tissue in a subject in need thereof. The methods include selecting and preparing a solid substrate for promoting cell or tissue growth or restored function for implantation, which solid substrate has a length and width or that promotes a tight fit within the boundaries of the implantation site and is further characterized by a height sufficient such that when a first terminus of said solid substrate is implanted within a bone in a site for implantation, a second terminus of said solid substrate is at a height at least 2 mm less than an articular cartilage layer surface or is proximal to a tide mark region in said implantation site and optionally applying a biocompatible polymer layer to an apical surface of said implant, which layer does not exceed the articular cartilage layer surface in height. Tools for implementation of optimal positioning are described including a unique tool (5-120) for trimming cartilage at the implantation site.

Provided is a highly safe articular cartilage regeneration material. This articular cartilage regeneration material contains: a scaffold-free artificial tissue in which synovial-derived mesenchymal stem cells cultured with a serum-free medium form a three-dimensional structure; and an extracellular matrix derived from mesenchymal stem cells. The articular cartilage regeneration material is implanted so as to be attached to a site of lost or damaged articular cartilage.

A formulation for use in treating osteoarthritis by modifying the shape change of bone(s) underlying articular cartilage is disclosed. The use comprises evaluating the bone shape of the patient's joint, injecting the patient with a peptide of SEQ ID No. 1 or applying other therapeutic interventions that can reduce the shape change of the bone(s) underlying articular cartilage, and thereafter evaluating the bone shape of the patient's joint.

The present invention relates to novel long non-coding RNAs (lncRNA) involved in the regulation of hypertrophy of chondrocytes, wherein RUNX2 was regulated at the post-translational level during hypertrophic differentiation of the chondrocyte cell line TC28a2 and two novel RUNX2-binding lncRNAs among numerous lncRNAs were identified, leading to the present invention. It is expected that bone-related diseases can be diagnosed by measuring the expression of the identified specific lncRNAs, and furthermore, the vascularization or calcification of articular cartilage can be effectively suppressed by inhibiting the hypertrophy of chondrocytes through inhibiting the expression of the identified specific lncRNAs, which is expected to be highly effective in the prevention or treatment of bone-related diseases.

The application provides a compound preparation for improving osteoporosis and osteoarthritis, a preparation method and application thereof, and belongs to the technical field of biological medicines. The compound preparation contains the following raw materials: glutathione, curcumin, calcium carbonate, vitamin D3, hydrolyzed collagen, and glucosamine sulfate; wherein the glutathione exists in the form of a cyclodextrin inclusion compound. The preparation method comprises the following steps: mixing the calcium carbonate, the hydrolyzed collagen, the glucosamine sulfate and excipients, then adding an equal amount of a premix of the glutathione cyclodextrin inclusion compound, the curcumin and the vitamin D3 in a stepwise manner, and performing dry granulation and tabletting to obtain the compound preparation. The compound preparation can simultaneously increase bone density, protect articular cartilage, reduce the levels of oxidative stress and inflammation, and can be used for preparing medicines or health foods for preventing or adjuvant treating osteoporosis and osteoarthritis. The application has a scientific formula, a stable process and a good application prospect.

The invention discloses an articular cartilage MRI (Magnetic Resonance Imaging) image intelligent enhancement and contrast optimization system, which belongs to the technical field of medical image processing and comprises a cartilage feature extraction module, a self-adaptive enhancement module, a contrast optimization module and a quality evaluation feedback module, the cartilage feature extraction module realizes cartilage region classification and structural analysis based on T2 relaxation time distribution features and multi-scale structure tensor features; the adaptive enhancement module performs differential enhancement according to the region type and the structural feature; the contrast optimization module realizes contrast dynamic optimization through nonlinear mapping and edge gradient adaptive adjustment; according to the articular cartilage MRI image display system and method, the display quality of the articular cartilage MRI image is remarkably improved, the cartilage display definition is improved by 73% or above compared with that of an original image, and a high-quality imaging basis is provided for accurate diagnosis of chondropathy.

A system and method for a ROSE-Reverdin / DARE osteotomy obviates severe deformities having a high intermetatarsal angle and which require closure via osteotomy (bone cut) of the first and / or fifth metatarsal in the midshaft or proximal aspect of the bone. The ROSE osteotomy is utilized to achieve this correction and is performed without need for fusion of the first metatarsal cuneiform joint. The ROSE osteotomy achieves correction of the intermetatarsal angle via rotation of the first and / or fifth metatarsal about a proximal based axis point. The Reverdin / DARE osteotomy is required to correct a deviated articular cartilage deformity at the head of the first metatarsal and / or additional slight correction of the IM angle. The Reverdin / DARE osteotomy achieves correction by wedging of the cut to realign the cartilage and translation of the head of the first metatarsal for further IM correction. A unique jig is used to achieve this cut and fixation.

An apparatus and methods are provided for a dermal cap for resurfacing a metatarsal head of the foot or a metacarpal head of the hand. The dermal cap comprises an outer surface having a convex shape, an inner surface having a concave shape, and a rounded peripheral surface joining the inner surface and the outer surface. The inner surface can be placed onto a bone head such that the outer surface provides a bone head surface resembling the original articular cartilage. The outer surface effectively restores the articular cartilage and thus relieves pain and facilitates better joint function. The dermal cap can be formed by stretching it over a rounded mandrel shaped similarly to the size and anatomy of the bone head to be treated. The dermal cap can be freeze-dried on the rounded mandrel to set the desired anatomy of the dermal cap.

This invention relates to a polyamino acid-based in-situ cartilage regeneration porous gel scaffold and its preparation method. Phenylboronic acid ester is esterified and linked with polyethylene glycol-modified Kartogenin to obtain phenylboronic acid ester-modified PEGKGN. The PEGKGN modified with phenylboronic acid ester is then grafted onto the side chains of polyamino acids with allyl polyethylene glycol to obtain a graft copolymer PLBPKA. The graft copolymer PLBPKA is blended with small molecules of Kartogenin and a silk fibroin solution to form a drug-loaded self-assembly network under shear force. Finally, the polyamino acid-based in-situ cartilage regeneration porous gel scaffold is constructed through ultraviolet light crosslinking and freeze-phase separation, which is a differentially released drug hydrogel scaffold. Compared with existing technologies, the scaffold of this invention has good biocompatibility and biocontrollable degradation, and can rapidly release drugs in inflammatory environments to promote stem cell migration; while in normal physiological environments, it slowly releases drugs to promote the differentiation of stem cells into cartilage. Therefore, it can promote in-situ regeneration of articular cartilage in the absence of exogenous stem cells.

The utility model discloses a mouse articular cartilage defect modeling micropore drill bit adjusting structure, which belongs to the technical field of drilling tools and comprises a mounting seat assembled at the output end of an electric drill pen, and a hole is formed in the middle of the mounting seat and a sleeve is welded in the middle of the mounting seat. According to the mouse articular cartilage defect modeling micropore drill bit adjusting structure, the locking block matched with the guide part is extruded when the guide part rotates and descends, so that the locking strip deforms and accumulates force, and the reamer is driven to abut against a hole in the drilling process; when the expanding drill rotates, the hole diameter is expanded through the stepped spiral groove of the expanding drill, and the drilling diameter is adjusted; therefore, the drilling depth and diameter are controllable, drill bits of different specifications do not need to be replaced, the operation time is shortened, the modeling efficiency is improved, the modeling quality is improved, the purchase and storage cost of the drill bits is reduced, finally, the flexibility, precision and efficiency of drilling and modeling operation are improved, the cost and the safety risk are reduced, and animal modeling experiment research is facilitated.

The invention belongs to the field of biological medicine or health food, and particularly relates to a composition for improving bone joint health and application thereof. Through wide research, the combination of the chondroitin sulfate sodium with small molecular weight and N-acetylglucosamine achieves unexpected synergistic effects in the aspects of inhibiting inflammatory factor level, improving arthralgia / stiffness, reducing joint swelling, maintaining articular cartilage health and the like; and a foundation is laid for developing products capable of effectively maintaining the health of bone joints.

This invention belongs to the field of bone repair materials technology, specifically relating to a bone repair implant based on a cold-cured resin-calcium glycerophosphate composite gel and its preparation method. The bone repair implant of this invention uses cold-cured resin as the main component of the gel, hyaluronic acid as an auxiliary component, and calcium glycerophosphate, citrate, calcium gluconate, and calcium amino acid as gel solidification promoters to form a bone repair implant with high elasticity, high toughness, and a certain strength. It has been proven that this implant can be used as a basic material for cartilage tissue repair, providing an effective option for the repair of articular cartilage and other cartilage tissues.

The invention discloses joint health-care soft sweets, and belongs to the field of health-care foods. Comprising hyaluronic acid or hyaluronate, a glucosamine compound without strongly acidic anions, a sugar body, a colloid, an additive and purified water. Compared with the prior art, firstly, glucosamine compounds, such as acetyl sugar or lemon glucosamine, which are milder in metabolism are selected as nutrient elements of the health-care soft sweets, so that the problem of stomach stimulation caused by introduction of sulfate radicals or chlorine radicals is fundamentally avoided, and damage to gastric mucosa can be reduced; secondly, the glucosamine and the hyaluronic acid are compounded according to a specific proportion, have a synergistic effect and can jointly protect articular cartilage and lubricate joints; and finally, through a specific material pretreatment method and process, the technical problems of high viscosity, moisture absorption, caking and the like caused by hyaluronic acid are solved, and the homogeneity, stability and industrial productivity of the composition in the soft sweets are ensured.

The invention discloses application of LRP1 in preparation of osteoarthritis medicines, and belongs to the technical field of biological medicines. The m6A methylase can regulate the immune homeostasis of macrophages and regulate chondrogenic differentiation of bone marrow mesenchymal stem cells through LRP1 mediated lipid metabolism reprogramming, so that repair and regeneration of articular cartilage are promoted, and a potential new immune metabolism dual regulation strategy is provided for treatment of osteoarthritis patients.

The invention belongs to the technical field of cartilage tissue engineering, and discloses a cartilage tissue engineering scaffold with a cross-scale fiber structure and a preparation method and application thereof. According to the method, a handheld electrospinning gun is used, and a coarse fiber grid layer and a non-woven fiber structure layer are sequentially formed on a defective cartilage model by controlling the distance between a gun head and the cartilage defect model; and soaking in a hydrogel solution, and curing to obtain the cartilage tissue engineering scaffold. According to the method provided by the invention, the cross-scale fiber structure scaffold can be rapidly prepared on the curved surface structure, and the scaffold can be matched with the articular cartilage defect boundary and curved surface characteristics; and moreover, the scaffold can be endowed with finer and smaller regular aperture, is beneficial to colonization of stem cells, and can be widely applied to repair of cartilage defects.

The invention relates to the technical field of oral disease animal model construction methods, in particular to a construction method of a traumatic temporomandibular ankylosis animal model. The construction method comprises the following steps: selecting an anterior auricular region on one side of an experimental subject, avoiding temporal superficial artery and vein, passively separating from front to back along a zygomatic arch to a joint capsule, exposing a mandibular condylar process neck and part of the zygomatic arch, and separating a condylar process after opening the joint capsule; the outer side attachment of the joint disc is separated through the joint gap, the peripheral attachment of the joint disc is cut off after the joint disc is exposed, and part or all of the joint disc is removed; the joint space is exposed through horizontal blunt separation of the joint capsule, part of fibrous cartilage on the condylar process head is removed, and fossa articular cartilage is damaged; after being sober, the animals eat soft food, and the traumatic temporomandibular joint ankylosis animal model is obtained. Modeling is carried out in the mode that the condyle process and the temporal bone glenoid fossa part fibrous cartilage and the articular disc are removed in a surgical operation mode, and the animal model established in a composite trauma mode fits the complex pathological environment of human diseases.

The application discloses a kind of superlubricity hydrogel microspheres, preparation method and application in the treatment of osteoarthritis, it is related to superlubricity hydrogel interface technical field, wherein, superlubricity hydrogel microspheres have core-shell structure, including core and shell;The core is sodium alginate hydrogel microsphere, and the shell is hydration layer.Sodium alginate hydrogel microsphere as core provides stable structural basis, and hydration layer as shell can keep moisture, thereby reducing friction during movement.The superlubricity hydrogel microspheres provided by the application have superlubricity, excellent biocompatibility and degradability, can provide buffer for interarticular cartilage friction, change sliding friction to rolling friction, reduce cartilage wear, and delay the progress of osteoarthritis.

Methods for treating joint diseases, disorders, and conditions, such as osteoarthritis, with a composition containing a therapeutically effective amount of tissue inhibitors of matrix metalloproteinases (TIMPs) are provided. The composition can contain TIMPs as part of amniotic fluid, or the TIMPs can be isolated from another source or recombinantly produced. The methods are particularly useful for treating osteoarthritis associated with degradation of articular cartilage by proteases. Also provided are methods of lubricating a joint, such as an osteoarthritic joint.

The invention discloses a non-denatured type II collagen peptide as well as a preparation method and application thereof, and belongs to the technical field of active collagen peptide preparation. According to the invention, the non-denatured II-type collagen peptide with biological activity is successfully prepared; according to the preparation method, strong acid, strong base and high temperature are not used, any organic solvent with toxic and side effects is not used, most grease is removed through multiple temperature adjustment, and the prepared non-denatured II type collagen peptide is high in purity, excellent in quality, good in safety and good in repairing, lubricating and anti-inflammatory effects on articular cartilage.

Disclosed herein are encapsulated microspheres comprising a plurality of cells, e.g., chondrocytes, where the plurality of cells is arranged into clusters. The disclosed encapsulated microspheres recapitulate many features of native articular cartilage and are able to be cultured on cartilage or bone to cure defects in the cartilage. Also disclosed herein are tissue constructs, orthopedic grafts, methods of generating the disclosed encapsulated microspheres, and methods of testing a candidate therapeutic.

This invention discloses a method for preparing a fusion protein of sialic acid-binding immunoglobulin-like lectin 6 extracellular fragment and human immunoglobulin G1-Fc fragment, and its application, belonging to the field of biomedical technology. The fusion protein consists of a functional fragment of the Siglec-6 extracellular region linked to the Fc fragment of human IgG1 via a peptide linker. Its amino acid sequence is selected from one of SEQ ID No. 1, SEQ ID No. 2, or SEQ ID No. 3, or a variant with more than 90% homology to the above sequences and retaining the biological activity of Siglec-6. The preparation method includes: cloning the Siglec-6 fragment and the coding sequence of the Fc fragment, constructing a recombinant expression plasmid and transfecting it into mammalian cells to induce expression, and purifying the target protein by affinity chromatography. The fusion protein vcSig6-Fc of this invention has extremely high structural stability and ligand binding affinity, can significantly inhibit the expression of inflammatory factors and matrix-degrading enzymes in chondrocytes, and shows excellent performance in promoting COL2A1 expression, thus exhibiting good protective effects on articular cartilage and therapeutic effects on rheumatoid arthritis.

This invention relates to a matrix stiffness-induced chondrogenic self-healing hydrogel, its preparation method, and its applications, belonging to the field of hydrogel preparation technology. The matrix stiffness-induced chondrogenic self-healing hydrogel of this invention utilizes biosafe and biocompatible natural polymer materials (sodium alginate, carboxymethyl chitosan, and silk fibroin). The network structure and matrix stiffness of the hydrogel are precisely controlled through intermolecular hydrogen bonds, dynamic covalent bonds, and a β-sheet synergistic strategy to prepare a matrix stiffness-induced chondrogenic self-healing hydrogel. The matrix stiffness-induced chondrogenic self-healing hydrogel prepared by this invention possesses the safety of a natural biomedical material, excellent self-healing properties, and the ability to induce stem cell chondrogenic differentiation and promote articular cartilage regeneration.

The present invention belongs to the field of biotechnology. Provided are a circular IL-10 mRNA nano-preparation, and a preparation method therefor and the use thereof. The circular IL-10 mRNA nano-preparation is prepared by means of the following method: 1, synthesizing a chemically modified circular IL-10 mRNA in vitro; and 2, preparing, by means of self-assembly, a circular IL-10 mRNA nano-preparation that can be delivered in vivo. The circular IL-10 mRNA inhibits injury of articular cartilage by means of regulating immune cells in the synovial membrane of an articular cavity, thereby achieving the effect of treating inflammatory joint diseases.

This invention relates to the field of organoid bioprinting technology, and discloses a method for preparing articular cartilage organoids, a bio-ink for encapsulating articular cartilage organoids, the preparation method thereon, and their applications. The preparation method includes: pretreating the raw material to be cultured in a basal culture medium containing a nanomedicine additive, wherein the nanomedicine additive chemically comprises mesoporous silica nanoparticles loaded with docetaxel and polyethyleneimine modified on the mesoporous silica nanoparticles; culturing the pretreated raw material in a cartilage organoid induction culture medium containing docetaxel; wherein the raw material to be cultured contains bone marrow mesenchymal stem cells. The articular cartilage organoids prepared by this method have a distinct cartilage-bone bio-interface, and the pretreatment process can help maintain the long-term phenotype of the cartilage organoids by inhibiting endochondral ossification during their development.

This invention discloses an inflammatory pretreatment xenogeneic synovial cell-derived vesicle, its preparation method, and its application in the treatment of osteoarthritis, belonging to the field of biomedical technology. The preparation method includes: obtaining and culturing rabbit synovial fibroblast-like cells; subjecting the cells to inflammatory pretreatment for 40-60 hours using a culture medium containing 70-80 ng / mL tumor necrosis factor-α (TNF-α); collecting the cell supernatant, and purifying it by differential centrifugation and ultracentrifugation to obtain the vesicles. The vesicles have a particle size distribution of 100-1000 nm, with a higher proportion of larger particles (>800 nm) than natural vesicles. This invention also provides a pharmaceutical composition containing these vesicles and their application in the preparation of drugs for treating osteoarthritis. Experiments show that these vesicles can significantly inhibit subchondral ossification and osteophyte formation, effectively protect articular cartilage, and have a therapeutic effect superior to natural vesicles, providing a novel cell-free therapeutic strategy for osteoarthritis.