300results about How to "Improve bioavailability" patented technology

The invention belongs to the technical field of cosmetics, and particularly relates to a cosmetic delivery system based on a natural wall material, a preparation method and application. The cosmetic delivery system is of a core-shell structure, a core is a cosmetic active component, and a shell layer is a composite polymer gel network of sodium alginate, sophorolipid and cellulose. The natural polysaccharide composite wall material is green and safe, has good biocompatibility and strong compatibility with cosmetic formulas, and does not irritate the skin. The delivery system can effectively protect cosmetic active ingredients from being damaged by illumination and oxygen, and the storage stability of the active ingredients is remarkably improved; meanwhile, slow release and targeted transdermal absorption of active ingredients are realized, and the bioavailability is improved.

This invention relates to the field of biopharmaceutical technology, providing a self-assembled peptide-rhein conjugate, its formulation, preparation method, and applications. The MMP-9-responsive self-assembled peptide-rhein conjugate of this invention, with the sequence Rhein-GFFPLGLAGACE, can respond to abnormally activated MMP-9 in atherosclerotic plaques, self-assembling in situ into a stable nanofiber structure at the lesion site. The rhein in this conjugate exhibits significant anti-inflammatory and antioxidant functions, but its water solubility and oral bioavailability are low. These are significantly improved after conjugation with the peptide, achieving targeted delivery and prolonging retention time. The introduction of the tripeptide ACE, which mimics the function of glutathione, further enhances the conjugate's ability to resist oxidative stress. Furthermore, this conjugate exhibits excellent anti-inflammatory and antioxidant effects, scavenging ROS, inhibiting inflammatory pathways, regulating macrophage phenotype, and inhibiting apoptosis, providing a new strategy for the treatment of atherosclerosis.

The invention discloses an application of engineered citrobacter freundii in fermentation synthesis of a stable body. The engineered citrobacter freundii is citrobacter freundii overexpressing a polyphosphate kinase gene Ppk1. The stable body prepared by fermenting the engineered citrobacter freundii is rich in polyphosphates and polyamines, particularly, the content of the polyamines can be up to 21wt%, the polyphosphates in the stable body can store the polyamines in an ionic bond combination mode, the combined polyamines are insoluble in water, a natural anion coating is generated, and the stability of the stable body is improved. And the sustained-release tablet is not easily taken by intestinal epithelial cells, so that the sustained-release tablet has a certain sustained-release effect. The medicine prepared by taking the compound as a main active ingredient shows a remarkable curative effect in ulcerative colitis treatment, has the comprehensive effects of reducing oxidative stress, enhancing the anti-inflammatory ability of a body and regulating immunity, is suitable for patients of all ages, and has outstanding application value and market prospect.

The invention belongs to the technical field of chemistry, and particularly relates to a water-based stable transdermal preparation based on a structural nanoemulsion and a preparation method of the water-based stable transdermal preparation. 1% to 10% of a polyether block copolymer; 5%-20% of a co-emulsion; 0.1% to 15% of an active ingredient; and the balance of deionized water. Through a compounding system of an amino acid surfactant and a gemini surfactant and in combination with the synergistic effect of a specific polyether block copolymer, a nanoemulsion drug delivery system with a stable structure is constructed. Through a compound system of the amino acid surfactant and the gemini surfactant and by combining the synergistic effect of the polyether block copolymer, a nano-emulsion drug delivery system with a stable structure is constructed, and the nano-emulsion drug delivery system has good long-term storage stability and temperature adaptability, is not easy to layer and is uniform in particle size distribution.

This invention discloses a multi-target synergistic anti-liver cancer medical food granule and its preparation method. The granule uses medicinal and edible homologous foods and novel resource foods as raw materials, and is composed of curcumin extract, esterified fat-soluble EGCG, Moringa leaf powder, yeast β-glucan, Astragalus extract, Schisandra extract, Salvia miltiorrhiza extract, Poria cocos powder, resistant dextrin, and erythritol in a specific ratio to construct a three-dimensional synergistic system of "direct killing-immune regulation-liver protection," which can induce ferroptosis in liver cancer cells, inhibit protective autophagy, activate the body's immunity, and protect liver cells. This invention employs directional extraction, multi-layer coating, and high-pressure fusion granulation technology. Nanoparticle liposome encapsulation, esterification modification, and pH-responsive enteric coating improve the stability and bioavailability of the components, solving the problems of poor water solubility, low absorption, and easy degradation of natural active ingredients. The granule has clear efficacy, high safety, and stable process, and can be used as a medical food for adjuvant therapy and nutritional support during the recovery period of liver cancer.

The present application relates to the technical fields of medical health food, and discloses a preparation device for naringin compound loaded by octenyl succinic acid modified porous starch ester, which comprises a preparation liquid ultrasonic mixing cylinder assembly, a mixing top cover assembly is arranged on the top of the preparation liquid ultrasonic mixing cylinder assembly, and a mixed liquid shaking and stirring assembly is arranged on the mixing top cover assembly; a rocker arm base assembly is rotatably arranged at the bottom of the preparation liquid ultrasonic mixing cylinder assembly; a preparation liquid mixing cylinder assembly is fixedly arranged on both sides of the top of the preparation liquid ultrasonic mixing cylinder assembly; the preparation liquid ultrasonic mixing cylinder assembly comprises a preparation liquid ultrasonic mixing cylinder, a plurality of mixing cylinder bottom arm tables are fixedly arranged on the preparation liquid ultrasonic mixing cylinder through reinforcing rib plates, and a bottom arm table main shaft rod is fixedly arranged on the mixing cylinder bottom arm table; an aeration pipe is fixedly arranged at the bottom of the preparation liquid ultrasonic mixing cylinder, and a liquid pump is fixedly arranged outside the bottom of the preparation liquid ultrasonic mixing cylinder. The present application has the characteristics of sufficient mixing.

The application provides a cold-dissolved liver-protecting medicinal tea and a preparation method and application thereof. The preparation raw materials of the cold-dissolved liver-protecting medicinal tea include green tea, rattan tea, ginseng, lotus leaves, mulberries and hawthorn. The preparation method comprises the steps of grouping extraction and spray drying of the preparation raw materials. The air inlet temperature of the spray drying is 130-150 DEG C, and the air outlet temperature is 75-85 DEG C. The grouping extraction comprises jointly extracting the ginseng, mulberries and hawthorn, and jointly extracting the green tea, rattan tea and lotus leaves in the presence of cyclodextrin. The cyclodextrin comprises one or more of beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin. The cold-dissolved liver-protecting medicinal tea is derived from natural plants, has a simple formula, is safe and effective, is convenient to use, and can retain active ingredients to the greatest extent.

This invention belongs to the field of functional substance research, specifically relating to the use of casein hydrolyzed peptide compositions in improving aging, and more specifically to their use in the preparation of products for improving aging. The casein hydrolyzed peptide compositions are obtained by enzymatically hydrolyzing casein solution using serine protease and flavor protease. Furthermore, in the casein hydrolyzed peptide compositions, the relative strength of total functional peptides is above 25%, the relative strength of casein phosphopeptides is above 9%, and the relative strength of functional peptides other than casein phosphopeptides is above 11%. This casein hydrolyzed peptide composition can effectively alleviate oxidative stress caused by aging, inhibit chronic low-grade inflammation, reduce aging-related damage to vital organs such as the liver and brain, improve the body's immune function, and improve the external phenotype of aging, thereby comprehensively exerting an anti-aging effect.

This invention relates to the fields of pesticide formulations and pesticide pest control, specifically to a composite nano-slow-release suspension of emamectin benzoate and chlorantraniliprole, its preparation, and application. The nano-slow-release suspension comprises emamectin benzoate, chlorantraniliprole, a nanocarrier, a wetting agent, a dispersant, a synergist, a thickener, an antifoaming agent, an antifreeze, a preservative, and water. The nanocarrier is an NX-CMCS & MOF composite nanocarrier. Spraying this composite nano-pesticide onto crops improves the adhesion and penetration of the pesticide on leaves, enhances its resistance to rain washout, and effectively kills major crop pests such as bollworms, diamondback moths, cowpea thrips, and rice planthoppers. Compared with traditional pesticide formulations, it has a significant effect of reducing dosage and increasing efficacy, and has broad application prospects in the field of agricultural pest control and promoting green agriculture.

The application provides a pentaethylenehexamine-beta-cyclodextrin grafted folic acid inclusion cannabidiol nanosphere, a preparation method and an application, the method first prepares a pentaethylenehexamine-beta-cyclodextrin grafted folic acid (PEHA-beta-CD-FA) targeting carrier, then cannabidiol (CBD) is mixed with the carrier in an ethanol-water mixed solvent in a certain proportion, a stable inclusion system is formed through ultrasonic-assisted inclusion + room-temperature light-avoiding stirring, and the system is purified through dialysis, freeze-drying or spray-drying, so that the spherical nanoparticles with uniform particle size of about 100 nm are obtained. The application solves the problems that CBD has extremely low water solubility, poor bioavailability and lacks targeted delivery, the obtained nanospheres have folic acid receptor active targeting, pH response drug release, high encapsulation rate and high colloidal stability, can significantly improve the solubility, dissolution rate and in-vivo targeted delivery efficiency of CBD, and are suitable for the fields of anti-inflammatory, neuroprotective and the like drugs, cosmetics and functional foods.

The invention discloses natural cereal and coarse cereal composite nutritional flour and a preparation method thereof, and relates to the technical field of flour preparation, the natural cereal and coarse cereal composite nutritional flour comprises the following raw materials: wheat flour, modified oat flour, modified corn flour, auxiliary coarse cereal flour and silicon dioxide; according to the invention, the modified oat flour is added, and through triple synergistic modification, the solubility and bioavailability of beta-glucan in oat are greatly improved, the water binding capacity and thermal stability of the composite flour are also enhanced, starch retrogradation is effectively inhibited, and the shelf life of a finished product is prolonged; pure physical modification does not have chemical residues, so that the composite flour is suitable for special crowds with diabetes, celiac disease and the like, and the defect that common coarse cereal composite flour is insufficient in functionality is overcome; the modified corn flour is added, the problems that common corn flour is low in dietary fiber solubility and poor in dough stability can be solved, a compact network is formed through synergy of natural polysaccharides, the water holding capacity and processing fluidity of the composite flour are improved, and water loss and caking in the cooking or baking process are reduced.

The present application relates to a crystal form of a compound of formula (I) and a preparation method and application thereof. Specifically, the present application provides a crystal form of a compound of formula (I), which has good stability, good drug efficacy, and good safety, and can be used for preparing a drug for treating or preventing feline transmissible enteric disease virus infection or coronavirus infection caused diseases.

The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.

This invention discloses a compound traditional Chinese medicine powder inhaler for respiratory tract infections and its preparation method, relating to the field of pharmaceutical formulation technology. The compound traditional Chinese medicine powder inhaler for respiratory tract infections is composed of polysaccharide composite gel microspheres, forsythia, scutellaria, honeysuckle, and isatis root. The polysaccharide composite gel microspheres are composed of seaweed sulfated polysaccharide, lentinan, calcium chloride, and curcumin. Seaweed sulfated polysaccharide, lentinan, curcumin, and calcium chloride cross-link to form a three-dimensional network spherical structure, improving the particle morphology and flowability of the micropowder. Furthermore, due to its good hygroscopic and moisturizing properties, it competitively reduces the moisture absorption of the micropowder, preventing aggregation and further improving flowability. In addition, the gel microspheres have excellent adhesion, adhering to the infection site after entering the respiratory tract, reducing expulsion losses due to respiration, maintaining an effective drug concentration at the infection site, and improving therapeutic efficacy.

The present application relates to the technical field of feed additive, and discloses a lactation-promoting functional feed for lactating sows containing high-efficiency plant lactogenic factors, which is made of the following raw materials: sodium-based montmorillonite, fenugreek extract, motherwort extract, ion intensity regulator, lysophosphatidylcholine, tributyl citrate and high-purity silicon dioxide, and is prepared through the following steps: mixing and drying sodium-based montmorillonite with the ion intensity regulator to prepare a regulation carrier; adding fenugreek and motherwort extract and mixing them by strong shearing to form an active core; atomizing and spraying a mixture of lysophosphatidylcholine and tributyl citrate on the surface of the core to form a functional layer; and finally adding high-purity silicon dioxide and mixing uniformly to obtain the feed. Through the construction of the structure with ion-pretreated montmorillonite as the core and the functional layer as the shell, the present application realizes the protection, sustained release and efficient absorption of the active ingredients, and improves the bioavailability.

This invention discloses a Tibetan bath composition compound and its preparation method, relating to the field of daily chemical products technology. The Tibetan bath composition compound consists of a ternary complex of scutellaria baicalensis hydroxypropyl-β-cyclodextrin nanoshell powder, Bacillus subtilis, Tibetan medicine composition, starch, anhydrous citric acid, sodium bicarbonate, calcium citrate, crospovidone, and magnesium stearate. Bacillus subtilis is used to ferment the ternary complex of scutellaria baicalensis hydroxypropyl-β-cyclodextrin nanoshell powder to obtain a fermentation-modified porous carrier, which is then loaded with the Tibetan medicine composition to form a Tibetan medicine inclusion powder. This not only achieves efficient drug delivery through a triple mechanism of biomodification, metabolite synergy, and structural optimization, but also forms a quaternary system with Terminalia chebula. The effervescent effect also enhances the penetration of Tibetan medicine components, which is beneficial for better exerting the antibacterial and anti-inflammatory effects of active substances, and significantly improving fungal infections and eczema-related skin problems.

The present application relates to the field of pharmaceutical preparations, in particular to a long-acting eye composite preparation for improving the symptoms of blepharitis. The preparation comprises a combination of two active ingredients in a therapeutically effective amount, and one or more ion-sensitive polymers or their complex systems, which are used to treat the symptoms of blepharitis caused by Demodex through a special double-cavity drug delivery bottle. By using a specific ion gel matrix concentration and regulating the viscosity of the drug solution before it reaches the eye, the limitations of traditional eye preparations are overcome, the retention time of the drug in the eye is prolonged, the bioavailability is improved, the probability of adverse events is reduced, and the medication compliance is increased.

This invention provides a food-grade ultramicroemulsion for improving nutrient stability, its preparation method, and its application, relating to the field of food technology. The food-grade ultramicroemulsion of this invention, by weight, comprises the following components: 5-20 parts polyol, 5-30 parts oil, 0.1-2 parts primary emulsifier, 0.01-0.5 parts co-emulsifier, and 60-90 parts water; the primary emulsifier is selected from at least one of polyglycerol fatty acid esters and mono- and diglycerol fatty acid esters; the co-emulsifier is selected from at least one of sucrose fatty acid esters, diacetyl tartaric acid mono- and diglycerides, phospholipids, and citrate fatty acid glycerides. The components are mixed and sheared under mild conditions of 50-65°C, resulting in an emulsion with a narrow particle size distribution, high stability, and high retention rate of fat-soluble nutrients.

The invention provides preparation and application of black cymbidium hookerianum vesicles and PDRN loaded on the black cymbidium hookerianum, the black cymbidium hookerianum vesicles are extracted from the black cymbidium hookerianum by taking the black cymbidium hookerianum as a source and combining an optimized differential-ultracentrifugation extraction process, and the black cymbidium hookerianum vesicles can be used for loading multiple active ingredients and can be used for preparing the PDRN loaded on the black cymbidium hookerianum vesicles. Particularly, the black cymbidium hookerianum vesicles are subjected to surface cation modification so as to be used for loading PDRN, and the black cymbidium hookerianum vesicles can be applied to the directions of cosmetics with functions of functionalization, formula compounding, oxidation resistance, aging resistance and the like.

The present application relates to a rare ginsenoside composition and a method and application thereof, wherein the ginsenoside composition comprises 0.02-0.1 mg / mL of S-Rg3, 0.009-0.029 mg / mL of R-Rg3, 0.087-0.245 mg / mL of Rg5 and 0.013-0.044 mg / mL of CK. The rare ginsenoside is prepared by fermentation, which comprises the following steps: step-by-step fermentation with yeast and lactic acid bacteria. Through the step-by-step fermentation method, the inherent ginsenoside is converted into rare ginsenoside, and 17 kinds of rare ginsenosides, including S-Rg2, R-Rg2, F1, F4, Rk3, F2, Rh4, S-Rg3, R-Rg3, PPT, Rg5, CK, S-Rh2, R-Rh2, Rk2, Rh3 and PPD, are obtained. The bioactivity of the fermented ginseng is higher, and the fermented ginseng can be applied to the food, medicine, health food and skin care product industries.

A eutectic comprising a compound of formula 1 and oxalic acid,

The invention relates to the technical field of traditional Chinese medicine preparations, and discloses a pear syrup-containing medicinal and edible wall-broken paste for treating heart-spleen deficiency, which is prepared from the following raw materials in parts by mass: 24 to 30 parts of compound medicine, 8 to 12 parts of lily, 100 to 120 parts of pear syrup, 12 to 18 parts of Chinese yam, 6 to 8 parts of semen euryales and 6 to 8 parts of semen coicis. Potential risks of non-edible raw materials are avoided, the pear syrup conforming to the national standard achieves dual effects of nourishing yin, moistening dryness and improving the taste, the industrial pain point that powder and a syrup body of a traditional syrup formula are not uniformly mixed is solved, and uniform distribution of components is ensured. A circulating cooling water and nitrogen double-control system is adopted in the whole process, the crushing temperature is strictly controlled to be 50 DEG C or below, and volatile effective components are reserved to the maximum extent.

The application belongs to the technical field of microcapsules, and particularly relates to a preparation method of a composite probiotic capsule, which comprises the following steps: uniformly mixing probiotic bacterial liquid, fructooligosaccharide, tea polyphenol, grape polyphenol, mannan oligosaccharide, isomaltitol and an emulsifier to obtain a core material; uniformly mixing glycerol and distilled water, heating to 60-80 DEG C, sequentially adding modified gelatin, hydroxypropyl starch and chitosan, stirring for 10-30 min, sterilizing, and cooling to room temperature to obtain a capsule material; mixing the core material and the capsule material, performing high-pressure homogenization treatment, and then performing spray drying to obtain the composite probiotic capsule; and the modified gelatin is prepared by acylation reaction of succinic acid derivatives and gelatin. The composite probiotic capsule provided by the application has excellent storage stability, antioxidant property, anti-gastric juice erosion capacity, and also has good enteric solubility and bioavailability.

The invention relates to the technical field of skin care, and discloses a sustained-release composition containing azelaic acid composite lipidosome and application of the sustained-release composition in acne treatment and skin repair. Comprising a composite liposome, an encapsulated active component and a dispersion medium, the composite liposome is composed of a lipid bilayer, the lipid bilayer comprises phospholipid, cholesterol and pegylated phospholipid, and the phospholipid is a mixture of hydrogenated soybean phospholipid and dipalmitoyl phosphatidylcholine; the encapsulated active component comprises a fat-soluble component and a water-soluble component; the water-soluble components comprise a hamamelis virginiana leaf water extract and a herba portulacae extract; the dispersion medium is a phosphate buffer solution; by optimizing lipid composition, introducing surface modification and constructing a multi-layer release system, the invention aims to synergistically exert multiple effects of antibiosis, anti-inflammation, cutin regulation, red fading, repair and the like, and provides an efficient, mild and integrated solution for acne and sequelae skin problems thereof.

This invention discloses a strain of *Lactobacillus plantarum* CCFM1487 and its prepared metagener for lowering lipids and alleviating fatty liver, as well as its application in converting cassiaside. It belongs to the fields of microbial technology and food and health product technology. This strain can efficiently convert cassiaside into cassiaside aglycone. The core of this invention lies in providing a metagener prepared from this strain through fermentation and inactivation treatment. Experiments in a high-fat diet mouse model have demonstrated that it significantly reduces serum total cholesterol and triglyceride levels, and can alleviate hepatic lipid deposition and improve hepatic steatosis. Based on this metagener, foods, medicines, or health products can be developed for the prevention and / or adjuvant treatment of metabolic disorders such as hyperlipidemia and non-alcoholic fatty liver disease, possessing significant application value.

This invention relates to a pH-responsive hydrogel composite dressing, its preparation method, and its application, belonging to the field of biomedical materials and drug delivery technology. The pH-responsive hydrogel composite dressing comprises a hydrogel matrix and engineered exosomes and platelet-rich plasma uniformly loaded within the hydrogel matrix. The hydrogel matrix comprises a three-dimensional dynamic cross-linked network formed by a Schiff base reaction between an amino-containing polysaccharide derivative and an oxidized aldehyde-containing polysaccharide derivative. The engineered exosomes are derived from donor cells subjected to pathogen-associated molecular pattern stress treatment. The dressing provided by this invention possesses both toxin capture and immunometabolic regulation functions, achieving synergistic therapeutic effects against MRSA-infected diabetic wounds.

The present application relates to the field of feed additive, and discloses a feed additive, a preparation method and application thereof.The feed additive contains astaxanthin, curcumin, vitamin C palmitate, vitamin E, vitamin D3, piperine and a carrier.The preparation method comprises the following steps: mixing astaxanthin, curcumin, vitamin C palmitate, vitamin E, vitamin D3, piperine and the carrier.The feed additive can significantly improve the stress resistance, immunity, antioxidant capacity and disease resistance of aquatic animals, and has significant economic benefits.

The application provides a sulfur-oxidizing bacteria-mediated carbon-free autotrophic denitrification water treatment method, and the high-performance sulfur-oxidizing bacteria with a polysulfide yield of 35%-42% is prepared through the dual-stress gradient domestication of the mass proportion of elemental sulfur in the sulfur source and the pH of the system; the high-activity bacterial suspension is prepared through centrifugal washing and buffer resuspension; the partitioned fluidized bed reactor is used, the compound sulfur source of elemental sulfur and sodium sulfide with nine waters is proportionally controlled in stages, and under the condition of no organic carbon source, the efficient autotrophic denitrification of the nitrogen-containing wastewater is realized. The denitrification efficiency is high, the operation stability is strong, no organic carbon source needs to be added, the simultaneous removal of multiple pollutants such as total phosphorus and suspended solids can be realized, the effluent quality can stably meet the high-standard discharge requirements, and the application is suitable for the advanced denitrification treatment of tail water of urban sewage treatment plants and industrial low-carbon-nitrogen-ratio wastewater.

This invention discloses a compound preparation for treating vascular dementia and its preparation method. The active pharmaceutical ingredient consists of protopanaxadiol and gallic acid in a 1:1 mass ratio, and the dosage form is liposomes. The preparation method includes extraction, mixing, liposome preparation, and optional freeze-drying steps: protopanaxadiol and gallic acid are extracted and purified from ginseng rhizome and Terminalia chebula fruit, respectively. After mixing, they are combined with phospholipids and cholesterol to prepare a lipid membrane. The membrane is then homogenized by hydration, low-temperature ultrasound, and high-pressure microfluidic jet to obtain a homogeneous liposome suspension, which can be further freeze-dried into a freeze-dried powder. The 1:1 composition of protopanaxadiol and gallic acid targets multiple points on the pathological mechanism of vascular dementia, showing significant therapeutic effects and broad application prospects. This preparation uses a liposome dosage form to improve the bioavailability of the active ingredient, the preparation process parameters are controllable, the active ingredient has high purity, the liposomes have uniform particle size, high encapsulation efficiency, and good stability.

This invention discloses a method for enhancing the antibacterial activity of Propionibacterium freudenreichii subsp. shermanii metabolites by adding a surfactant to a culture medium. The method uses Propionibacterium freudenreichii subsp. shermanii NBRC No. 12426 as the experimental strain and Pichia anomala SICC 2.894 as the indicator strain. A surfactant is added to the fermentation medium to enhance the antibacterial activity of the metabolites of Propionibacterium freudenreichii subsp. shermanii against the indicator strain Pichia anomala. Based on the culture conditions and method of Propionibacterium freudenreichii subsp. shermanii in this invention, the optimal concentration of the surfactant Tween-80 is selected as 0.3%, which significantly enhances the antibacterial activity of the metabolites of Propionibacterium freudenreichii subsp. shermanii.