133results about How to "Reduced activity" patented technology

This invention relates to an air electrode material and its preparation method. The preparation method includes: using metal salts of Ce, Pr, La, Ba, and Sr, metal salts of Co, and a fluorine source as raw materials, and preparing a fluorine-containing perovskite precursor according to a stoichiometric ratio; pretreating the precursor and then sintering it to obtain a composite powder; the composite powder includes a fluorine-containing perovskite main phase and a fluorine-containing CeO2 nanophase; pulverizing and sieving the composite powder to obtain air electrode powder; the fluorine-containing perovskite main phase is an A-site high-entropy perovskite oxide; the fluorine-containing CeO2 nanophase is precipitated through the self-assembly of some Ce elements during sintering; the fluorine-containing CeO2 nanophase is dispersed inside the main phase grains or at grain boundaries, and forms a strongly coupled heterogeneous interface with the main phase. This invention solves the technical problems of poor surface exchange activity, bulk diffusion transport, and long-term stability of existing air electrodes.

The invention discloses a lactobacillus brevis with broad-spectrum bacteriostatic activity and a separation method and application thereof, and belongs to the technical field of microorganisms, in particular to a lactobacillus brevis AW04 with broad-spectrum bacteriostatic activity, which is characterized in that the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, and the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection. The address is Wuhan University Campus, Wuchang District, Wuhan City, Hubei Province; the postcode is 430072; the preservation number of the strain is CCTCC M 20241970; the preservation date is September 13, 2024. The strain provides a novel, safe and efficient strain resource for developing microecological preparations, feed additives or veterinary drugs for preventing and treating bacterial diarrhea of animals such as calves and the like, and has important value for reducing the use of antibiotics in the breeding industry and coping with bacterial drug resistance crisis. The strain can be produced on a large scale by using the existing fermentation technology and is convenient to apply.

The application belongs to the technical field of tunnel soil body reinforcement, and particularly discloses a device and method for reinforcing soil body of a tunnel starting well portal by injecting microbial slurry, which comprises a storage component, a transmission component and an injection component, the storage component comprises two constant-temperature boxes, and the two constant-temperature boxes are respectively used for placing bacterial liquid and cementing liquid; the transmission component comprises a multi-connection micro high-pressure pump, and two independent liquid channels are arranged in the pump; the injection component comprises a grouting guide pipe and a rotating grouting head, the grouting guide pipe comprises two hollow semi-cylindrical pipes, and the longitudinal straight faces of the two hollow semi-cylindrical pipes are attached together to form a cylinder with two independent longitudinal cavities; the two independent longitudinal cavities are respectively connected with the two constant-temperature boxes through different liquid channels; a plurality of spray holes are longitudinally and uniformly arranged on the arc faces of the hollow semi-cylindrical pipes; and the rotating grouting head is used for driving the grouting guide pipe to rotate. The application can reduce the internal loose problem of the soil body reinforced by the microorganism, and improve the reinforcement effect of the soil body of the tunnel starting well portal.

The invention relates to the technical field of preparation of integral foam metal catalysts, in particular to a lattice distortion induced catalyst for catalytic oxidation of acetone and a preparation method thereof, and the preparation method comprises the following steps: step 1, selecting and cutting raw materials; step two, acid pretreatment and cleaning; 3, preparing a solution; step 4, hydro-thermal synthesis; 5, washing and drying the catalyst; and 6, performing electrothermal induction on the catalyst. The lattice distortion induced monolithic catalyst for electrothermal concerted catalytic oxidation of acetone is prepared in a hydrothermal synthesis manner, active component loading and electronic structure regulation are synchronously realized, energy consumption is remarkably reduced in catalytic oxidation of acetone, the problems of catalyst sintering and active center inactivation are relieved, and the method is suitable for industrial production. And the activity at low temperature is improved.

The application provides an antibacterial neural electrode based on a high-surface-potential zirconium oxide layer and a preparation method thereof. The electrode comprises, from bottom to top, a bottom zirconium oxide film layer, a first insulating layer, a metal layer, a second insulating layer, a top zirconium oxide film layer and an electrode modification layer. A distal end of the metal layer is partially exposed to form an electrode point, and the electrode modification layer covers the electrode point. The bottom zirconium oxide film layer and the top zirconium oxide film layer are subjected to high-surface-potential treatment and exhibit a positive surface potential in a physiological environment. The positive surface potential can destroy the cell membranes of bacteria with negative charges through electrostatic action after the neural electrode is implanted in vivo, and inhibit the formation of microglial cells and astrocytes. The damage to the membranes of mammalian cells (such as neurons) with neutral electricity is extremely small. The application realizes active antibacterial function through the high-surface-potential zirconium oxide film layer, ensures long-term effective contact between the electrode and neurons, and thus realizes high-quality, long-term stable electrophysiological recording and stimulation function of the neural electrode.

The utility model discloses a multifunctional cell collector, including sample protection pipe and setting operation head at sample protection pipe top, the operation head bottom end center place is fixed with the collection rod, the collection rod perpendicular plug -in at sample protection pipe center place inside, and the length of collection rod is less than the length of sample protection pipe, the collection rod bottom end outside is fixed with cell collection swab head, through adding detachable, reusable cold storage protection device outside sample protection pipe, when not using, the device is frozen in the freezer, and the inside silica sol is in frozen state, after cell collection, collection rod is inserted to sample protection pipe, and the frozen cold storage protection device is sleeved outside sample protection pipe, and low temperature is provided continuously by silica sol, slows down cell metabolism, prevents active reduction or metamorphism, guarantees sample original state and integrity, improves quality, lays the foundation for detection analysis, and its characteristics reduce cost, reduce waste.

The invention belongs to the technical field of heavy metal pollution treatment, and discloses a method for reducing soil cadmium activity and wheat grain cadmium content. According to the method, the low-cadmium-accumulation wheat variety is selected, the composite conditioner is applied, the low-cadmium-accumulation wheat variety is Jimai 22, and the composite conditioner is composed of mineral source potassium fulvate and zinc fertilizer. The complex passivation effect of humic acid components in mineral source potassium fulvate on soil heavy metal is utilized, the ion antagonism effect of zinc and cadmium and the low cadmium accumulation characteristic of Jimai 22 are combined, the cadmium content of wheat grains is synergistically reduced to 0.1 mg / kg or below, meanwhile, the cadmium content of the soil can be remarkably reduced, and the soil with cadmium exceeding the standard can be repaired. The treatment method provided by the invention has the advantages of simple operation, easily available raw materials, low cost, stable synergistic cadmium reduction effect and the like.

The invention discloses a preparation method and application of a porous iridium nano-metal catalyst, and belongs to the field of material synthesis and electro-catalysis. Iridium acetylacetonate and copper acetylacetonate are used as precursors, an IrCu alloy nanosphere precursor is prepared through solvothermal reaction in an ethylene glycol and surfactant system, copper is removed through acid etching, and the porous iridium nano spherical catalyst (Ir-NS) is obtained. The catalyst has a three-dimensional porous spherical structure, active sites are concentrated on the surfaces of nanospheres and the edges of the walls of internal mesopores, catalytic active sites are enhanced due to the iridium nanosphere structure and uniform site exposure density, oxygen bubbles are quickly released, and the active sites are prevented from being covered by the bubbles. In an acid medium and proton exchange membrane water electrolysis anode oxygen evolution reaction, the catalyst shows low overpotential and long-term operation stability under low iridium loading capacity. The method is simple in process and low in cost, does not need an additional hard template, and has a good industrial application prospect.

The invention relates to the technical field of biological medicine, and particularly discloses application of combination of an IGF1R inhibitor and immunotherapy in preparation of a medicine for treating lung cancer, the combination of the IGF1R inhibitor OSI-906 and an immune checkpoint inhibitor can effectively enhance the curative effect of small cell lung cancer immunotherapy, and compared with treatment with a single immune checkpoint inhibitor or the IGF1R inhibitor, the curative effect of the IGF1R inhibitor OSI-906 and the curative effect of the IGF1R inhibitor OSI-906 can be effectively enhanced. The IGF1R inhibitor combined with the immune checkpoint inhibitor can remarkably inhibit the growth level of tumors, the growth level of the tumors is obviously lower than that of the single use of common antibody drugs, because the IGF1R inhibitor can reduce the activity of endothelial cell IGF1 / IGF1R pathways and destroy the vascular structure of small cell lung cancer, the combined immunotherapy of the IGF1R inhibitor can remarkably enhance T cell infiltrating tumors, and the immune checkpoint inhibitor can effectively inhibit the tumor growth. Therefore, growth of small cell lung cancer cells is inhibited, the tumor immunotherapy effect of the immune checkpoint inhibitor is improved, and tumor growth is better inhibited.

This invention provides bfrA Single gene knockout or bfrA Genes and bfrB Double gene knockout of Neisseria gonorrhoeae and its applications involve the field of biotechnology. Research has found that... bfrA Single gene knockout and bfrA and bfrB Double gene knockout of Neisseria gonorrhoeae resulted in increased formation of vesicles on the outer membrane, disruption of the cell wall and cell membrane, reduced adhesion and invasion abilities, decreased bacterial activity, decreased ATP levels, and weakened ability to infect mice. Therefore, knocking out Neisseria gonorrhoeae... bfrA Gene or bfrA and bfrB The dual-gene assay can be used to prepare products for the production of gonococcal outer membrane vesicles and to construct low-pathogenic gonococcal models.

This invention belongs to the field of material surface treatment technology, specifically relating to a wafer polishing slurry, its preparation method, and its application. The wafer polishing slurry comprises abrasives, oxidants, dispersants, surfactants, pH adjusters, and water. The components of the wafer polishing slurry work synergistically, shortening polishing time and improving polishing efficiency when applied to wafer polishing. Furthermore, it significantly improves the surface quality of the polished wafer, markedly reducing scratches and defects, and lowering surface roughness to below 0.15 nm, thus meeting the surface quality requirements of fields such as power electronics and radio frequency devices.

This invention relates to the fields of environmental monitoring and biosafety technology, and in particular to a viral aerosol sampling patch for face masks, comprising: a capture layer for intercepting viral aerosol particles in the air; a culture layer disposed inside the capture layer, the culture layer comprising a culture unit supporting the virus to maintain activity and replicate in the presence of a host, a host bacterium sensitive to the virus, and a visual indicator indicating viral replication or host response; and a base layer disposed inside the culture layer; the sampling patch is fixed to the face mask through the base layer, and viral aerosol collection is achieved without external power through the capture layer, and countable detection results are generated after sampling through the culture layer; utilizing the airflow and humidity conditions naturally formed during wearing, effective interception, activity preservation, and visual detection of viral aerosols are achieved without complex equipment and additional operating steps, and the operation is simple and repeatable.

This invention discloses a trifluoromethylselenoquinone derivative, its preparation, and its application. The structure of the trifluoromethylselenoquinone derivative is shown in formula (I). Wherein, the R1 group is selected from H, halogens, C... 1‑5 Alkyl, C 1‑5 One of alkoxy and acetyl groups, and R2 group selected from monosubstituted or unsubstituted phenyl and thiophene groups. This invention is the first to develop a photochemical synthesis method for trifluoromethylselenoquinone derivatives. This method uses visible light as a clean driving force and has the advantages of mild conditions and environmental friendliness. This invention successfully introduces the trifluoromethylseleno group into the naphthoquinone core, constructing a novel functional molecule that integrates the advantages of the naphthoquinone skeleton, fluorine atom, and chalcogenide triple structure, enriching the library of fluorine-containing chalcogenide heterocyclic compounds and providing a novel structural unit for innovative drug development. The compound synthesized in this invention exhibits excellent VDR agonist activity and has good drug development prospects. (I)

The utility model relates to the technical field of indoor environment adjustment, and discloses an indoor ecological health-care system which comprises an ecological room, and an embedded air pipe is fixedly mounted on the inner side of the ecological room. According to the indoor ecological health care system, by arranging the filter plate, the electrostatic dust collection net, the photocatalytic net, the negative ion generator, the cleaning assembly and other assemblies, the traditional passive purification mode of filter net interception is changed, the filter plate and the electrostatic dust collection net can effectively intercept particulate pollutants, the photocatalytic net can actively inhibit or decompose the activity of microorganisms, and the purification effect is improved. The negative ion generator generates negative ions, suspended particles in the air can be adsorbed and coagulated, the negative ions can be combined with bacteria, viruses and the like in the air, the structure of the air is destroyed, the microbial activity is reduced, multiple assemblies act synergistically, the purification treatment efficiency is greatly improved, the risk of secondary pollution in the high-humidity environment is reduced, frequent replacement of a filter screen is not needed, and the cost is reduced. The maintenance cost and the operation complexity are reduced, and a healthier and fresher indoor air environment is created.

This invention belongs to the field of scalp care formulation technology, and discloses a soothing, antibacterial, anti-hair loss, and hair-strengthening formulation with peptides and plant active ingredients, and its preparation method. The formulation includes a stabilized peptide-plant active ingredient pre-complex liquid, a matrix moisturizer, a scalp conditioner, a thickener, a pH adjuster, a preservative, and deionized water. The pre-complex liquid is prepared by pre-dispersion, weakly acidic pre-complexation, and stabilization treatment of licorice extract, scutellaria baicalensis extract, rosemary extract, hydrolyzed keratin peptides, plant-derived hydrolyzed peptides, glycerin, 1,3-propanediol, β-glucan, and sodium hyaluronate. In preparation, a plant active ingredient pre-dispersion liquid is first formed, then a low-molecular-weight peptide complex is added, followed by β-glucan and sodium hyaluronate for stabilization, and finally mixed with the formulation matrix and the pH is adjusted. This invention is beneficial for improving the dispersion stability, scalp and hair surface retention, and antibacterial persistence of plant active ingredients, and is suitable for soothing, antibacterial, anti-hair loss, and hair-strengthening care.

The utility model relates to a forestry pest control device belongs to forestry pest control technical field. This forestry pest control device, include: support rod, the top of support rod installs the collection mechanism, the top of collection mechanism in collection frame is fixedly connected with the fixed plate, the bottom of fixed plate installs the insect trapping lamp, the top of fixed plate installs the photovoltaic board, the above-mentioned killing mechanism a plurality of atomizing nozzles will insecticidal liquid atomization spray, and under the action of fan wind force makes the connecting pipe drive atomizing nozzle swing, is favorable to make the insecticidal liquid that atomizing nozzle sprays better diffusion, thereby makes the pest that was sucked into the collection frame around and the insecticidal liquid that sprays contact, is favorable to directly carry out killing to part of the pest that was sucked in smaller, reduces the activity of bigger pest simultaneously, makes the escape ability of bigger pest when being sucked in weaken, thereby makes bigger pest better be sucked into the collection frame in fan, thereby improved the processing effect to pest.

The invention discloses a preparation method and application of a livestock breeding feed using fruit wine residues as raw materials, which are applied to the field of feed preparation, and aims to solve the problems of low resource utilization rate of fruit wine residues, waste of feed resources, low breeding benefits and the like. According to the method, fruit wine residues are pretreated and precisely compounded, a mineral adsorbent is added to optimize a structure so as to carry out mixing and tempering, multi-stage synergistic fermentation and dynamic regulation and control of a fermentation process, and intermittent stirring and weak forced ventilation are carried out during regulation and control; through the raw material classification and blending, the conditioning technology and the multi-stage synergistic fermentation technology, harmful gas emission in the fermentation process is effectively controlled, the stable fermentation environment is maintained, and the utilization rate of protein and microelements in the feed is remarkably increased; the fruit wine residues which are originally difficult to treat are converted into the high-quality livestock feed with balanced nutrition, so that wine brewing byproducts are turned into wealth, and the problem of environmental pollution caused by wastes is solved.

The application discloses a kind of auxiliary devices for semen collection, it is related to the technical field of male medical examination, including disposable collection cylinder, the rear side of disposable collection cylinder is provided with triangular plate, the left and right sides of triangular plate are provided with adjustable auxiliary mechanism, the top of disposable collection cylinder is provided with sound blocking mechanism, the rear side of triangular plate is symmetrically provided with opening sleeve plate left and right, disposable collection cylinder can quickly restore to vertical state in clamped state after semen collection is completed, on the one hand, compared with the current artificial hand-held disposable collection cylinder semen collection mode, the structure used in the present scheme can release the hands of patient during semen collection, to facilitate better semen collection, on the other hand, after semen collection is completed, disposable collection cylinder can be quickly restored to vertical state, so that the semen in disposable collection cylinder can be prevented from leaking to the maximum extent.

The application belongs to the technical field of biological medicine, and more particularly relates to nanoparticles TK-PSBs / CBD@Met, a preparation method therefor and application thereof. The nanoparticles TK-PSBs / CBD@Met are nanoparticles formed by wrapping cannabidiol and metformin with thione-modified lung surfactant biomimetic liposomes. In vitro experiments have confirmed that the nanoparticles TK-PSBs / CBD@Met can effectively inhibit the fibrosis process of BEAS-2B cells induced by silicon dioxide. Animal experiments have shown that the nanoparticles TK-PSBs / CBD@Met can significantly improve the damage to the lung tissue structure and abnormal deposition of collagen caused by SiO2 exposure, and alleviate the pathological progression of silicosis fibrosis.

The invention discloses a preparation method of a carbon dioxide hydrogenation reaction catalyst. The preparation method of the oxygen carrier comprises the following steps: placing one or more of particles manually loaded with a copper element or natural ore particles and blank alumina pellets in a fluidized bed reactor according to a ratio, introducing methane gas, and jointly fluidizing at 600 DEG C for 2 hours; and after the reaction is finished and cooling, introducing nitrogen, and adjusting the gas speed until particles are layered to obtain the product catalyst. According to the method, inert particles which are originally wasted in the chemical looping combustion industry can become core components for preparing the catalyst, a special carrier does not need to be additionally purchased, the raw material cost of the catalyst is remarkably reduced, active elements on the surfaces of the prepared particles are uniformly loaded, the wear rate is smaller than 0.2% / h, and the method is a catalyst preparation method oriented to industrial application.

This application relates to a polypropylene composite material, its preparation method and application, and cables. By weight, the polypropylene composite material comprises the following components: 60-80 parts polypropylene, 20-40 parts ungrafted elastomer, 0.5-1.5 parts grafted elastomer, 0.01-0.2 parts β-nucleating agent, and 3-6 parts hydrophilic agent; the hydrophilic agent includes at least one selected from ethylene-acrylic acid copolymer, polyvinyl butyral, polyacrylic acid, polyvinyl alcohol, and ethylene vinyl alcohol copolymer. The components in this polypropylene composite material interact with each other, effectively improving the water-tree resistance of the polypropylene composite material, while also exhibiting high tensile strength and elongation at break.

The application discloses a kind of core-shell structure nanometer powder toughened zirconia ceramics and preparation method thereof, belong to ceramic material technical field, the method includes using three-dimensional network gel to realize washing-free co-precipitation method to prepare high-activity, ZrO2@SiO2Core-shell structure nanometer zirconia raw material powder, and stable agent is mixed ball milling, drying, sieving, shaping, cold isostatic pressing and gets green body, green body is placed in vacuum furnace and sintering, annealing and obtains toughened zirconia ceramic target product, realizes the efficient preparation of jade quality feeling simulation ceramic tooth of integral penetration.The application avoids the repeated washing steps of precursor in the traditional co-precipitation preparation process, reduces the introduction of impurities, the sintering temperature is lower, the powder yield is high, the process is simple, the cost is low, and is suitable for the industrial production of toughened zirconia ceramics.

The application provides a preparation method of nanometer silicon, which comprises the following steps: mixing a reducing metal and silicon halide, heating under a sealed condition to maintain the reducing metal in a molten state, stirring, and obtaining a reaction product; mixing the reaction product with a higher alcohol with hydrophobicity and then standing to obtain a reaction mixture; mixing the reaction mixture with water and stirring, standing to form a water layer and a hydrophobic colloidal layer, obtaining the hydrophobic colloidal layer, and drying to obtain nanometer silicon. The application is based on a molten metal reduction method, uses active reducing metal and silicon halide as raw materials, heats to maintain the reducing metal in a molten state in the reaction process, and reacts with the silicon halide to prepare nanometer silicon. Then, the prepared nanometer silicon is protected and separated by using the method of higher alcohol grafting. The higher alcohol can reduce the activity of the surface of the nanometer silicon, prevent the nanometer silicon from being oxidized in the process of air or water washing, and reduce the generation of silicon oxide impurities.

The utility model relates to the technical field of electrified railway application, in particular to a multifunctional anti-bird cover for an electrified railway, which comprises a first mounting sheath and a second mounting sheath which are arranged oppositely, and the sides, close to each other, of the first mounting sheath and the second mounting sheath are detachably connected. The first mounting sheath and the second mounting sheath are hollow, a mounting sheath cavity is formed between the first mounting sheath and the second mounting sheath, and one end of the first mounting sheath extends forwards to be provided with an arc-shaped cover body. According to the utility model, the structure is novel, the design is ingenious, the moving or staying frequency of birds on the umbrella skirts of the insulator chain is reduced, the amount of bird droppings on the umbrella skirts of the insulator chain is reduced, the influence of the bird droppings on the insulation performance of the insulator chain is reduced, the reliability and safety of an overhead line on an electrified railway are improved, and a certain shielding effect is achieved; and external corrosion is reduced, so that the multi-functional effect is achieved.

The application discloses an anti-aging recombinant humanized collagen composition and a preparation method and application thereof, and belongs to the technical field of biomedicine. The composition comprises a recombinant humanized collagen, a plant oil and a plant exosome freeze-dried powder, the recombinant humanized collagen and the plant oil are bonded into ionic liquids. The composition can improve the transdermal permeability of the recombinant humanized collagen and improve the in-vitro normal-temperature storage stability of the plant exosome. The application further provides a preparation method of the composition. The recombinant humanized collagen and the plant oil are atomized into positively-charged particles and negatively-charged particles respectively through an electrostatic atomization method, so that the activation energy of acid-base neutralization reaction of the two is reduced, and the two are bonded into ionic liquids through proton exchange.

The utility model discloses a device for quickly removing Brucella in small piercing-sucking mouthpart or filing-sucking mouthpart insects, which belongs to the field of biotechnology, and comprises a glass bottle, a degerming liner and a bottle cap, the color of the glass bottle is blue or yellow, the degerming liner is arranged in the glass bottle, the degerming liner is made of a transparent material, and the bottle cap is arranged in the glass bottle. The bottle cap is in threaded connection with the top end of the glass bottle, and a gauze element is arranged on the bottle cap. The insect killing device is simple in structure and convenient to use, insects are attracted through the blue or yellow glass bottle, the insects are attached to the bacteria removing film of the bacteria removing inner container, bacteria removing is conducted on the insects through the bacteria removing liquid on the bacteria removing film, the activity of the insects is reduced, and the survival rate during bacteria removing is improved.

This invention provides a method for manufacturing dibenzylamine. Benzonitrile and a reaction solvent are mixed in a high-pressure reactor, and a hydrogenation reaction is carried out under the action of a ruthenium-iron bimetallic catalyst supported on cerium oxide to obtain a crude product. After cooling and filtering the catalyst, the crude product is purified by distillation to obtain the final dibenzylamine. This method features mild reaction conditions, high atom utilization, and meets the requirements of clean and green production.

The application discloses an interfering RNA for inhibiting ALDH2 gene expression and application, belongs to the field of gene biological medicine, and relates to the interfering RNA, wherein the double-stranded siRNA comprises a sense strand and an antisense strand, and the double-stranded siRNA is selected from any one of SEQ ID NO. 1-2, SEQ ID NO. 3-4 and SEQ ID NO. 5-6. The interfering RNA can effectively inhibit ALDH2 gene expression in colorectal cancer cells, provides a new choice for preventing or treating colorectal cancer carrying an APC gene defect / mutation based on the principle of synthetic lethality, and provides a new target for drug research and development for rectal cancer treatment.

The invention discloses an application of paclitaxel in preparation of an anti-breast cancer drug. The invention proposes and verifies that taxafen effectively down-regulates WDYHV1 protein expression of tamoxifen drug-resistant breast cancer cells and induces ferroptosis of cancer cells for the first time, so that the activity of tamoxifen drug-resistant tumor cells is remarkably reduced, the development of breast cancer is inhibited, and a new direction is provided for treatment of tamoxifen drug-resistant lung cancer.

The invention belongs to the field of biological medicine, and discloses a mesenchymal stem cell-derived exosome and active factor secretion composition and a directional preparation method thereof. According to the method, umbilical cord tissues of healthy mammals are used as raw materials, primary stem cells are separated through enzymolysis, and amplification and passage are conducted to 3-5 generations through an alpha-MEM culture medium; a serum-free induction culture medium containing multiple functional components is adopted for directional induction, a conditioned culture medium is collected, gradient centrifugation and ultrafiltration concentration are performed, and the protein concentration is calibrated to be 2 mg / mL, so that a finished product is obtained. The product can synergistically realize moisturizing, anti-wrinkle and tightening effects. Specifically, the exosome and active factor secreted composition is a composite system which is obtained by separating and concentrating mesenchymal stem cells from a conditioned medium of the mesenchymal stem cells after the mesenchymal stem cells are directionally induced by the method and is mainly composed of the exosome, growth factors self-secreted by the stem cells, cell factors and macromolecular active protein.