23 results about "Gene deletion" patented technology

The invention discloses a fusarium venetianum engineering strain as well as a construction method and application thereof, relates to the technical field of gene engineering, and aims to solve the problem of low fungal hypha protein yield, chitin synthetase FvChs4 gene of fusarium venetianum is deleted, the classification name of the fusarium venetianum engineering strain is fusarium venetianum, and the fusarium venetianum engineering strain has the characteristics that the fusarium venetianum engineering strain has a nucleotide sequence shown in SEQ ID NO: 1 and a nucleotide sequence shown in SEQ ID NO: 2 and a nucleotide sequence shown in SEQ ID NO: 3. The fusarium venanii engineering strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 27, 2025, and the preservation number is CGMCC No. 42015. Through targeted knockout of the FvChs4 gene, consumption of a carbon source by cell wall synthesis is reduced, and directional enrichment of the carbon source to protein synthesis is realized, so that the yield of hypha protein is increased.

The application discloses a Toxoplasma gondii DAHPS gene deletion vaccine strain and application thereof. The application provides a Toxoplasma gondii gene knockout strain, which is obtained by directly knocking out a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase TgDAHPS gene of the Toxoplasma gondii through a gene editing technology, the gene ID of the TgDAHPS gene is TGGT1_221260, and the nucleotide sequence is shown as SEQ ID NO. 1. The gene deletion strain Δdahps provided by the application can grow normally in vitro, does not reproduce in a host in vivo, and is almost non-toxic. After immunization of the Δdahps strain, the host can be induced to have good immune protection. The Δdahps has the advantages of weak virulence and almost no reproduction in vivo as the attenuated vaccine strain of the Toxoplasma gondii, can improve the resistance of the host to wild Toxoplasma gondii, and can be used for preventing the infection of the Toxoplasma gondii in humans and animals, and has important significance for developing more Toxoplasma gondii vaccines.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The invention provides a method for preventing or treating diseases related to CSNK2B gene deletion. The disclosure also provides a CSNK2B protein or a variant thereof, a nucleic acid molecule encoding a CSNK2B protein or a variant thereof, an expression cassette comprising a nucleic acid molecule encoding a CSNK2B protein or a variant thereof, a vector comprising a nucleic acid molecule encoding a CSNK2B protein or a variant thereof, a virus, such as an AAV particle, expressing a CSNK2B protein or a variant thereof, and a method for preparing the CSNK2B protein or a variant thereof. According to the present invention, the use of the cells expressing the CSNK2B protein in the preparation of drugs for treating or preventing CSNK2B gene deletion-related diseases in subjects is provided, and the CSNK2B gene deletion-related diseases are selected from the group consisting of Rett syndrome, autism spectrum disorder (ASD), Porier-Bienvenu neurodevelopment syndrome (POBINDS), epilepsy or broad-spectrum development retardation, or the use of the cells expressing the CSNK2B protein in the preparation of the drugs for treating or preventing the CSNK2B gene deletion-related diseases in the subjects,

The application discloses a rice OsNAR1 application of the gene or the coded protein in regulating rice resistance to Magnaporthe oryzae, and relates to the technical field of rice Magnaporthe oryzae prevention and treatment. OsNAR1 The CDS nucleotide sequence of the gene is shown as SEQ ID No. 2. OsNAR1 The application finds that the rice gene OsNAR1 plays an important role in the process of rice resistance to Magnaporthe oryzae, the number of leaf spots on the gene deletion mutant is less, the gene can be used for screening rice strains resistant to Magnaporthe oryzae, the expression amount of a basic resistance marker gene in the gene deletion mutant is significantly up-regulated, and it is indicated that OsNAR1 negatively regulates the expression of the basic resistance gene and can be used for preventing and treating Magnaporthe oryzae.

The invention discloses a toxoplasma gondii gene deletion strain ME49 [delta] TGZS and an application thereof in preparation of a gene engineering inactivated vaccine. The toxoplasma gondii gene deletion strain ME49 [delta] TGZS is prepared by taking a toxoplasma gondii strain ME49 as a parent strain and knocking out a TGZS gene in the toxoplasma gondii strain ME49; the nucleotide sequence of the TGZS gene is as shown in SEQ ID NO: 1. The strain can maintain normal polypide growth and replication capacity under an in-vitro culture condition, after inactivation treatment, the strain loses proliferative activity but maintains a complete polypide morphological structure, and the strain is compatible with an aluminum hydroxide adjuvant to prepare a vaccine preparation. According to the vaccine, by activating the specific immune response of a terminal host cat, the sexual reproduction cycle of the toxoplasma gondii in the intestinal epithelial cells of the cat is effectively blocked, and the formation and discharge of oocysts are remarkably inhibited, so that the propagation chain of the toxoplasma gondii is controlled from the source. The invention provides a new effective means for prevention and control of toxoplasmosis.

The invention discloses a Brucella bacterium capable of generating a lipoid A vaccine adjuvant and an application of the Brucella bacterium. According to the invention, recombinant plasmids for expressing BacA and LpxL genes are constructed, and are transformed into Brucella through electric shock, so that a double-gene knockout strain is successfully obtained. By knocking out BacA, an ultra-long fatty acid chain of lipoid A is shortened, an organism is induced to generate an inflammatory reaction, and by knocking out LpxL, the inflammatory reaction is reduced, so that the effect of the immunologic adjuvant is achieved. LPS is extracted for in-vitro stimulation analysis, and it is found that the inflammatory response of double-gene deletion is reduced compared with that of single-gene deletion. Animal experiments show that double-gene deletion can induce an organism to generate good immune response and induce relatively high cellular immunity and humoral immunity, and a new thought is provided for research and development of gram-negative bacterium inactivated vaccines.

The invention provides a method for improving the yield of thaxtomin A through a streptomyces scabies SCAB82261 gene. The method comprises the following steps: deleting the streptomyces scabies SCAB82261 gene through genetic engineering to obtain a thaxtomin A high-yield strain, and carrying out fermentation production; wherein the nucleotide sequence of the SCAB82261 gene is as shown in SEQ ID NO. 1, and the coded amino acid sequence is as shown in SEQ ID NO. 2. The invention has the following advantages: the thaxtomin A biosynthesis negative regulation gene SCAB82261 is screened, and the PaaK protein encoded by the thaxtomin A biosynthesis negative regulation gene SCAB82261 can catalyze the degradation of aromatic compounds such as phenylalanine; when the streptomyces scabies SCAB82261 gene is deleted, a high-yield strain of the thaxtomin A can be obtained, and a technical support is provided for improving the yield of industrial production of the thaxtomin A.

This invention discloses a method for rice to withstand heat stress. RMI1 Genes and their applications. Among them... RMI1 The nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence of the protein it encodes is shown in SEQ ID No. 2; this invention verifies RMI1 The function of the gene, RMI1 protein is located in the cell nucleus, rmi1-1 In mutants RMI1 The deletion of 33 base pairs in the gene results in the loss of 11 amino acids in the RMI1-1 protein, making... rmi1-1 High temperatures during the seedling stage cause a large number of abnormal chromosomes to adhere and fragment during the late mitotic phase of root tip cells, resulting in stunted plant growth and development. rmi1-1 High temperatures during the reproductive growth period of plants lead to a large number of chromosome fragments and adhesions in the late stage of meiosis I, resulting in sterility; high temperatures also affect the interaction between RMI1-1 protein and topoisomerase TOP3α and helicase RECQ4. rmi1-cr3 Frameshift mutations lead to abnormal embryo and endosperm development; overexpression RMI1 This invention can enhance the heat resistance of rice. RMI1 The analysis of gene function provides genetic resources for the breeding of heat-resistant rice varieties.

The invention provides an application of a CsSTE12 gene in inhibition of Philospora piricola. The nucleotide sequence of the CsSTE12 gene is shown as SEQ ID NO.1, and the coding amino acid sequence of the CsSTE12 gene is shown as SEQ ID NO.2. The invention also provides an application of the CsSTE12 gene in inhibition of Philospora piricola in inhibition of Philospora piricola. According to the CsSTE12 gene deletion mutant disclosed by the invention, the CsSTE12 gene is knocked out, so that the obtained CsSTE12 gene deletion mutant cannot form a normal spore structure, and spores cannot fall off from hyphae, and the CsSTE12 deletion is proved to regulate the morphology of the spores of the helminthosporium basicola and influence the production of the spores. Molecular exploration is carried out from the perspective of pathogenic bacteria, and the application has important guiding significance in research on prevention and treatment of plant diseases caused by wheat root rot helminthosporium.

The present application belongs to the field of plant disease control research, and relates to a cucumber multi-host coelomyces effector protein CcSP1, a coding gene thereof and application. The present application provides a new cucumber multi-host coelomyces effector protein CcSP1, a coding gene thereof and application. The nucleotide sequence of the coding gene of the multi-host coelomyces effector protein CcSP1 is shown as SEQ ID NO. 1. Transient expression of the CcSP1 gene in N. benthamiana can inhibit hypersensitive necrosis caused by N. benthamiana. A gene deletion mutant ΔCcSP1 is constructed by homologous recombination, and the deletion of the gene does not affect the vegetative growth, but significantly reduces the pathogenicity when invading the cucumber leaves. It is shown that CcSP1 is involved in the lesion expansion of the plant after the invasion of the multi-host coelomyces, and plays an important role in the pathogenic process of the multi-host coelomyces. The present application has important significance for analyzing the related molecular mechanism of cucumber coelomyces leaf spot and establishing the comprehensive control technology strategy of the plant cucumber coelomyces leaf spot.

The application relates to application of a maize ZmECT3 gene in regulating heat tolerance of plants, belongs to the technical field of plant gene breeding, and discloses that the nucleotide sequence of the ZmECT3 gene is shown as SEQ ID NO. 1, and the amino acid sequence of a protein coded by the gene is shown as SEQ ID NO. 2. Through analysis of maize plants with a ZmECT3 gene deletion and Arabidopsis plants with overexpression of the ZmECT3 gene, it is found that the ZmECT3 gene can regulate heat tolerance of plants, and the application provides important theoretical significance and application value for creating new heat-tolerant maize germplasm and heat-tolerant maize molecular breeding.

This invention discloses tomatoes WRKY52 The application of genes in regulating plant resistance to insect pest stress belongs to the field of biotechnology. WRKY52 The nucleotide sequence of the protein-coding region of the gene is shown in SEQ ID NO.1. This invention discloses tomato for the first time. WRKY52 The role of genes in regulating plant resistance to insect pests, especially bollworm, and the use of CRISPR / Cas9 gene editing technology to obtain tomatoes WRKY52 The study found that the mutant with gene deletion exhibited reduced resistance to the cotton bollworm, proving... WRKY52 The application of genes as positive regulators in improving the resistance of tomatoes to insect pest stress can be used for the breeding of insect-resistant tomato varieties. This invention provides more gene resources and new ideas for plant molecular stress resistance genetic breeding and genetic modification of plant stress tolerance.

The invention discloses a monkey pox virus triple fluorescence PCR nucleic acid detection kit, a detection method and application. The kit adopts a single-tube triple detection system and comprises (a) a monkey pox virus universal detection primer probe group and a targeted F3L and G2R gene conserved region; (b) a Clade Ib subtype detection primer probe group which targets D14L and A36R gene deletion feature regions; (c) a Clade II subtype detection primer probe group, which is used for targeting J2R and D18L gene feature sequences; and (d) an internal standard detection group which targets the human RNase P gene. By optimizing the concentrations of the primers and probes and four-channel fluorescent labels (FAM universal type, VI-Clade Ib, ROX-Clade II and CY5 internal label), single-tube synchronous detection is realized. The leak detection risk is reduced by adopting a double-target redundancy design, virus screening and subtype identification (Clade Ib / II) can be completed through single detection, and an internal standard system monitors the quality of a sample in the whole process. The kit is suitable for clinical early diagnosis, epidemic prevention and control and strain traceability.

The invention discloses a primer and a probe for a fluorescent PCR method for identifying and detecting an African swine fever wild strain and a gene-deleted attenuated strain. The nucleotide sequence of the detection primer is as shown in SEQ ID NO.1-9. The detection kit provided by the invention can specifically detect the wild strain and the gene-deleted attenuated strain of the African swine fever virus, and has a remarkable application value for epidemiological monitoring of the African swine fever virus.

The invention discloses an application of a tomato SlMNSI1 gene in regulation and control of fruit quality. The nucleotide sequence of the gene is shown as SEQ: NO.1. The invention also discloses an application of the tomato SlMNSI1 gene in regulation and control of fruit quality. The SlMNSI1 gene knockout stable genetic material is obtained through a CRISPR / Cas9 gene editing technology, and the influence of the SlMNSI1 gene knockout stable genetic material on the tomato fruit quality is researched. Results show that the deletion of the SlMNSI1 gene can lead to advanced maturation of tomato fruits, significant increase of ethylene release rate and soluble solid content, reduction of fruit size, but significant increase of contents of fructose, glucose, sucrose and various aldehydes and alcohols, thereby significantly improving fruit flavor. The SlMNSI1 gene plays an important role in regulation and control of fruit development and flavor substance accumulation, and a new gene resource and an application approach are provided for creating a new variety of early-maturing, small-fruit-type and high-flavor tomatoes.

The invention discloses a gene for promoting maturation of tomato fruit plants and a regulation and control method thereof. The nucleotide sequence of the gene is shown as Seq No.1. According to the invention, a complete coding sequence of the SlMST1 gene is obtained from tomato, a target joint is designed, a trimming and connecting technology is utilized, a target segment is connected to a CRISPR / CAS9 carrier, an agrobacterium infection method is utilized to transform a plant, a gene editing plant is obtained, the gene editing plant is analyzed, and a result shows that tomato fruit maturation can be promoted after the gene is deleted.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an OsGRX6 gene in improving the toxicity tolerance of plants to ammonium nitrogen. The nucleotide sequence of the OsGRX6 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the OsGRX6 gene is as shown in SEQ ID NO. 2. The invention discloses that the OsGRX6 gene can be used for regulating and controlling the tolerance of the plant to the ammonium nitrogen toxicity for the first time; an OsGRX6 gene deleted rice plant is obtained through a CRISPR / Cas9 gene editing technology; osGRX6 gene overexpression is carried out, such that an OsGRX6 gene overexpression rice plant is obtained; plant experiment results show that the tolerance of a rice root system to high ammonium can be improved by deletion of the OsGRX6 gene, and the tolerance of the rice root system to high ammonium is reduced by overexpression of the OsGRX6 gene; the invention has important significance for cultivating a new rice variety which is resistant to ammonium nitrogen toxicity.

The invention discloses a method for constructing a low-beauvericin cordyceps sobolifera strain based on a non-resistance marker technology and application of the low-beauvericin cordyceps sobolifera strain, and belongs to the technical field of fungus molecular biology. In order to obtain a low-beauveria-bassiana cordyceps sobolifera strain (Cordyceps canhua KIVI with a preservation number of CCTCC NO: M2025637) without a resistance marker, the preparation method comprises the following steps: firstly, carrying out double enzyme digestion on a plasmid pPK2-bar to remove a herbicide-resistant gene bar, and inserting an upstream region and a downstream region of a coding region of cordyceps sobolifera orotic acid nucleotide decarboxylase URA3 into the plasmid to obtain a knockout plasmid pPK2-URA3 of the URA3. And then obtaining the URA3 gene deleted cordyceps sobolifera strain through a homologous recombination technology and the screening effect of 5-fluoroorotic acid (5-FOA) on the uridine / uracil auxotroph strain, so as to obtain the URA3 gene deleted cordyceps sobolifera strain. The content of the beauveria bassiana in the obtained cordyceps sobolifera strain meets the requirement (the content of the beauveria bassiana is less than or equal to 3mg / kg) of the'announcement of 15 kinds of'three-new foods' of national health commission about cordyceps sobolifera sporocarp (artificially cultivated) and the like '(No.9 in 2020).

The application of a rice gene in regulating phosphorus homeostasis, the nucleotide sequence of the rice OsSPL17 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein of the rice OsSPL17 gene is shown as SEQ ID NO. 2. By cloning the rice gene OsSPL17, a transgenic vector is constructed, and OsSPL17 gene overexpression rice and OsSPL17 gene deletion rice are obtained. The phenotypic traits of the transgenic rice are determined, and it is found that compared with the wild type rice, the phosphorus content of the transgenic rice changes, the overexpression rice has a lower phosphorus absorption rate in a high phosphorus environment, and the degree of high phosphorus toxicity of the plant is reduced; and the phosphorus absorption rate of the deletion rice increases in a low phosphorus environment, and the phosphorus utilization rate of the rice is improved. It is shown that the OsSPL17 gene plays an important regulatory function in the phosphorus utilization of the rice, maintains the phosphorus homeostasis of the rice, and is helpful to improve the phosphorus fertilizer utilization rate.

The invention discloses application of a rice OsNAR1 gene or a protein coded by the rice OsNAR1 gene to regulation and control of resistance of rice to pyricularia oryzae, and relates to the technical field of prevention and control of pyricularia oryzae. The CDS nucleotide sequence of the rice OsNAR1 gene is as shown in SEQ ID No. 2. Research finds that the rice gene OsNAR1 plays an important role in the rice blast resistance process of rice, the number of disease spots on leaves of a gene deletion mutant is smaller, the gene can be used for screening to obtain rice blast bacteria resistant rice strains, the expression quantity of basic resistance marker genes in the gene deletion mutant is remarkably increased, and the expression quantity of the basic resistance marker genes in the gene deletion mutant is remarkably increased. The expression of the basic resistance gene is negatively regulated by the OsNAR1, and the rice blast can be prevented and controlled.

This invention relates to the field of genetic engineering technology and provides a method for increasing lincomycin production by modifying the SLCG_1979 gene in *Streptomyces lincomyces*. The method involves deleting the SLCG_1979 gene, which encodes a TetR family transcription factor, from the *Streptomyces lincomyces* strain through genetic engineering, thereby obtaining a high-yielding lincomycin-producing strain. The resulting strain is then used for fermentation to produce lincomycin. The nucleotide sequence of the SLCG_1979 gene is shown in SEQ ID NO. 1. This invention also provides an application of the above-mentioned method for increasing lincomycin production in industrial strains. The advantage of this invention is that deleting the SLCG_1979 gene on the *Streptomyces lincomyces* chromosome through genetic engineering can yield a high-yielding lincomycin-producing strain, providing technical support for increasing lincomycin fermentation yield.

The application belongs to the technical field of plant genetic engineering, and particularly relates to OsGRX6 Application of a gene in improving tolerance of plants to ammonium nitrogen toxicity. OsGRX6 The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. OsGRX6 The gene can be used for regulating the tolerance of plants to ammonium nitrogen toxicity: by CRISPR / Cas9 gene editing technology OsGRX6 Gene deletion rice plants; by OsGRX6 Gene overexpression, obtain OsGRX6 Gene overexpression rice plants; plant experiment results show that deletion OsGRX6 The gene can improve the tolerance of rice root system to high ammonium, and overexpression OsGRX6 The gene reduces the tolerance of rice root system to high ammonium; the application has important significance for cultivating new rice varieties with tolerance to ammonium nitrogen toxicity.