11 results about "Gene deletion" patented technology

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The application discloses a rice OsNAR1 application of the gene or the coded protein in regulating rice resistance to Magnaporthe oryzae, and relates to the technical field of rice Magnaporthe oryzae prevention and treatment. OsNAR1 The CDS nucleotide sequence of the gene is shown as SEQ ID No. 2. OsNAR1 The application finds that the rice gene OsNAR1 plays an important role in the process of rice resistance to Magnaporthe oryzae, the number of leaf spots on the gene deletion mutant is less, the gene can be used for screening rice strains resistant to Magnaporthe oryzae, the expression amount of a basic resistance marker gene in the gene deletion mutant is significantly up-regulated, and it is indicated that OsNAR1 negatively regulates the expression of the basic resistance gene and can be used for preventing and treating Magnaporthe oryzae.

The invention discloses a toxoplasma gondii gene deletion strain ME49 [delta] TGZS and an application thereof in preparation of a gene engineering inactivated vaccine. The toxoplasma gondii gene deletion strain ME49 [delta] TGZS is prepared by taking a toxoplasma gondii strain ME49 as a parent strain and knocking out a TGZS gene in the toxoplasma gondii strain ME49; the nucleotide sequence of the TGZS gene is as shown in SEQ ID NO: 1. The strain can maintain normal polypide growth and replication capacity under an in-vitro culture condition, after inactivation treatment, the strain loses proliferative activity but maintains a complete polypide morphological structure, and the strain is compatible with an aluminum hydroxide adjuvant to prepare a vaccine preparation. According to the vaccine, by activating the specific immune response of a terminal host cat, the sexual reproduction cycle of the toxoplasma gondii in the intestinal epithelial cells of the cat is effectively blocked, and the formation and discharge of oocysts are remarkably inhibited, so that the propagation chain of the toxoplasma gondii is controlled from the source. The invention provides a new effective means for prevention and control of toxoplasmosis.

The invention provides a method for improving the yield of thaxtomin A through a streptomyces scabies SCAB82261 gene. The method comprises the following steps: deleting the streptomyces scabies SCAB82261 gene through genetic engineering to obtain a thaxtomin A high-yield strain, and carrying out fermentation production; wherein the nucleotide sequence of the SCAB82261 gene is as shown in SEQ ID NO. 1, and the coded amino acid sequence is as shown in SEQ ID NO. 2. The invention has the following advantages: the thaxtomin A biosynthesis negative regulation gene SCAB82261 is screened, and the PaaK protein encoded by the thaxtomin A biosynthesis negative regulation gene SCAB82261 can catalyze the degradation of aromatic compounds such as phenylalanine; when the streptomyces scabies SCAB82261 gene is deleted, a high-yield strain of the thaxtomin A can be obtained, and a technical support is provided for improving the yield of industrial production of the thaxtomin A.

This invention discloses a method for rice to withstand heat stress. RMI1 Genes and their applications. Among them... RMI1 The nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence of the protein it encodes is shown in SEQ ID No. 2; this invention verifies RMI1 The function of the gene, RMI1 protein is located in the cell nucleus, rmi1-1 In mutants RMI1 The deletion of 33 base pairs in the gene results in the loss of 11 amino acids in the RMI1-1 protein, making... rmi1-1 High temperatures during the seedling stage cause a large number of abnormal chromosomes to adhere and fragment during the late mitotic phase of root tip cells, resulting in stunted plant growth and development. rmi1-1 High temperatures during the reproductive growth period of plants lead to a large number of chromosome fragments and adhesions in the late stage of meiosis I, resulting in sterility; high temperatures also affect the interaction between RMI1-1 protein and topoisomerase TOP3α and helicase RECQ4. rmi1-cr3 Frameshift mutations lead to abnormal embryo and endosperm development; overexpression RMI1 This invention can enhance the heat resistance of rice. RMI1 The analysis of gene function provides genetic resources for the breeding of heat-resistant rice varieties.

The application relates to application of a maize ZmECT3 gene in regulating heat tolerance of plants, belongs to the technical field of plant gene breeding, and discloses that the nucleotide sequence of the ZmECT3 gene is shown as SEQ ID NO. 1, and the amino acid sequence of a protein coded by the gene is shown as SEQ ID NO. 2. Through analysis of maize plants with a ZmECT3 gene deletion and Arabidopsis plants with overexpression of the ZmECT3 gene, it is found that the ZmECT3 gene can regulate heat tolerance of plants, and the application provides important theoretical significance and application value for creating new heat-tolerant maize germplasm and heat-tolerant maize molecular breeding.

This invention discloses tomatoes WRKY52 The application of genes in regulating plant resistance to insect pest stress belongs to the field of biotechnology. WRKY52 The nucleotide sequence of the protein-coding region of the gene is shown in SEQ ID NO.1. This invention discloses tomato for the first time. WRKY52 The role of genes in regulating plant resistance to insect pests, especially bollworm, and the use of CRISPR / Cas9 gene editing technology to obtain tomatoes WRKY52 The study found that the mutant with gene deletion exhibited reduced resistance to the cotton bollworm, proving... WRKY52 The application of genes as positive regulators in improving the resistance of tomatoes to insect pest stress can be used for the breeding of insect-resistant tomato varieties. This invention provides more gene resources and new ideas for plant molecular stress resistance genetic breeding and genetic modification of plant stress tolerance.

The invention discloses a monkey pox virus triple fluorescence PCR nucleic acid detection kit, a detection method and application. The kit adopts a single-tube triple detection system and comprises (a) a monkey pox virus universal detection primer probe group and a targeted F3L and G2R gene conserved region; (b) a Clade Ib subtype detection primer probe group which targets D14L and A36R gene deletion feature regions; (c) a Clade II subtype detection primer probe group, which is used for targeting J2R and D18L gene feature sequences; and (d) an internal standard detection group which targets the human RNase P gene. By optimizing the concentrations of the primers and probes and four-channel fluorescent labels (FAM universal type, VI-Clade Ib, ROX-Clade II and CY5 internal label), single-tube synchronous detection is realized. The leak detection risk is reduced by adopting a double-target redundancy design, virus screening and subtype identification (Clade Ib / II) can be completed through single detection, and an internal standard system monitors the quality of a sample in the whole process. The kit is suitable for clinical early diagnosis, epidemic prevention and control and strain traceability.

The invention discloses a primer and a probe for a fluorescent PCR method for identifying and detecting an African swine fever wild strain and a gene-deleted attenuated strain. The nucleotide sequence of the detection primer is as shown in SEQ ID NO.1-9. The detection kit provided by the invention can specifically detect the wild strain and the gene-deleted attenuated strain of the African swine fever virus, and has a remarkable application value for epidemiological monitoring of the African swine fever virus.

The invention discloses a gene for promoting maturation of tomato fruit plants and a regulation and control method thereof. The nucleotide sequence of the gene is shown as Seq No.1. According to the invention, a complete coding sequence of the SlMST1 gene is obtained from tomato, a target joint is designed, a trimming and connecting technology is utilized, a target segment is connected to a CRISPR / CAS9 carrier, an agrobacterium infection method is utilized to transform a plant, a gene editing plant is obtained, the gene editing plant is analyzed, and a result shows that tomato fruit maturation can be promoted after the gene is deleted.

The application belongs to the technical field of plant genetic engineering, and particularly relates to OsGRX6 Application of a gene in improving tolerance of plants to ammonium nitrogen toxicity. OsGRX6 The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. OsGRX6 The gene can be used for regulating the tolerance of plants to ammonium nitrogen toxicity: by CRISPR / Cas9 gene editing technology OsGRX6 Gene deletion rice plants; by OsGRX6 Gene overexpression, obtain OsGRX6 Gene overexpression rice plants; plant experiment results show that deletion OsGRX6 The gene can improve the tolerance of rice root system to high ammonium, and overexpression OsGRX6 The gene reduces the tolerance of rice root system to high ammonium; the application has important significance for cultivating new rice varieties with tolerance to ammonium nitrogen toxicity.