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581 results about "Pseudorabies" patented technology
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Aujeszky's disease, usually called pseudorabies in the United States, is a viral disease in swine that has been endemic in most parts of the world. It is caused by Suid herpesvirus 1 (SuHV-1). Aujeszky's disease is considered to be the most economically important viral disease of swine in areas where classical swine fever (hog cholera) has been eradicated. Other mammals, such as cattle, sheep, goats, cats, dogs, and raccoons, are also susceptible. The disease is usually fatal in these animal species.
Method for preparing vaccine by editing pseudorabies virus genomes based on CRISPR/Cas9 and Cre/lox systems and application of method
ActiveCN104894075AReduce disease lossEasy to operateAntiviralsViruses/bacteriophagesMCherry fluorescent proteinBiology
The invention discloses a method for quickly preparing a vaccine by editing pseudorabies virus genomes based on a CRISPR / Cas9 gene editing system and a Cre / lox recombination system and an application of the method. According to the method, the CRISPR / Cas9 gene editing system is used for synchronously and efficiently recombining a GFP gene and a mCherry gene to a pseudorabies virus gE gene site and a TK gene site respectively to obtain conditional deletion strains of a gE gene and a TK gene; after purification, the Cre / lox system is used for cutting off extraneous GFP and mCherry genes in the pseudorabies virus recombinant virus genome so as to perform purification quickly to obtain a live pseudorabies virus vaccine lack of gE / TK genes; multiple genes are operated at the same time, so that multiple rounds of flows for knocking out multiple genes in the conventional method are reduced to one round; and meanwhile, the efficient edition of virus genes by using the CRISPR / Cas9 and Cre / lox systems simplifies about thirty generations of plaque purification processes into 3-4 generations, so that the preparation efficiency of the virus vaccine is greatly improved, and the method provides a strong guarantee for effectively preventing and controlling the larger-range popularization of variant pseudorabies viruses and reducing heavy economic losses.
Owner:武汉都为康生物科技有限公司
Porcine pseudorabies virus virulent strain, and gene deletion vaccine strain thereof and applications thereof
ActiveCN102994458AEffective preventionEffective therapeuticMicroorganism based processesAntiviralsRabiesMicrobacterium
The invention discloses a porcine pseudorabies virus virulent strain, and a gene deletion vaccine strain thereof and applications thereof. The porcine pseudorabies virus virulent strain is named as HeN1, the microbial preservation number of the porcine pseudorabies virus virulent strain is CGMCC NO.6656, the deleted gE gene obtaines the gene deletion vaccine strain rPRV-gE-EGFP+ on the basis of the virulent strain HeN1, and the microbial preservation number is CGMCC NO.6657. The virulent strain can be prepared into inactivated vaccine (single vaccine or combined vaccine), the gene deletion vaccine strain rPRV-gE-EGFP+ can be prepared into activated vaccine or inactivated vaccine (single vaccine or combined vaccine) and the like, so that porcine pseudorabies can be effectively prevented or cured, or the gene deletion vaccine strain rPRV-gE-EGFP+ can be prepared into a diagnosis reagent for diagnosing the porcine pseudorabies. The gene deletion vaccine strain rPRV-gE-EGFP+ has the advantages of being good in safety, high in protection efficiency, beneficial to differential diagnosis.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Porcine pseudorabies virus (PRV) variant PRV-ZJ01 and application thereof
ActiveCN103627678AImprove securityImproving immunogenicityMicroorganism based processesAntiviralsRabiesEngineering
Owner:NANJING AGRICULTURAL UNIVERSITY
Porcine pseudorabies virus gene deletion strain, vaccine composition, and preparation method and application of vaccine composition
ActiveCN103923884ASymptoms relieved or improvedMicroorganism based processesAntiviralsVirus antigenTGE VACCINE
The invention provides a porcine pseudorabies virus gene deletion strain, a vaccine composition, and a preparation method and an application of the vaccine composition. The vaccine composition comprises an immunizing dose of an attenuated livetotivirus antigen and an inactivated totivirus antigen of the porcine pseudorabies virus gene deletion strain or its culture. The vaccine composition can effectively induce the antibody production, can effectively protect pigs, and can be used as a marking vaccine to effectively differentiate wild strains and vaccine strains.
Owner:PU LIKE BIO ENG
Multiple real time fluorescence quantifying PCR method for detecting porcine circovirus, porcine parvovirus, porcine pseudorabies virus and classical swine fever virus
InactiveCN101260442ALow costImprove application stabilityMicrobiological testing/measurementClassical swine fever virus CSFVRegression analysis
The invention discloses a multiplex real-time fluorescent quantitation PCR method capable of detecting porcine circovirus, porcine parvovirus, porcine pseudorabies virus and hog cholera virus at the same time. The method of the invention detects that the four virus are all in good linear relations at the same time, all ten times serial dilution points of constructed normal plasmid are all in one straight line, CT value and copy number are in good linear relation, and regression analysis shows that the related coefficient of the CT value and the copy number is R<2> more than 0.99.The multiplex real-time fluorescent quantitation PCR method has the advantages of excellent specificity, sensibility and stability, which can rapidly, sensitively and differentially detect the four virus with serious harm to the economy and can be used for early diagnosis of virus infection.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Pseudorabies TK*/gE*/gI* gene dificiency mark live vaccine and preparation method thereof
The present invention discloses a construction of three-gene defected recombinant pseudorabies virus (PrV) strain, vaccine prepared by said strain, method for constructing said strain and method for preparing said vaccine. The described recombinant pseudorabies virus strain defects genes of TK.gE ang gI, and contains no exogenous gene. The described vacine belongs to the freeze-dried live vaccine made up by virus liquid containing said invention and gelatin. Said invented live vaccine can be inoculated on the piglet, slaughter pig and sow with farrow, and can obtain obious immune effect. Said vaccine has good biological safety, and can be used for preventing and curing pseudorabies.
Owner:HUAZHONG AGRI UNIV
Recombinant porcine pseudorabies virus TK/gE double-gene deletion strain
The invention separates a porcine pseudorabies virus GDXX strain, and a separating strain is served as a female parent for deleting a thymidylate kinase (TK) gene and a gE gene through a molecular biology means in sequence, thus obtaining recombinant virus PRV / TK- / gE-. The recombinant virus provided by the invention is used as a vaccine strain for preparing safe and efficient porcine pseudorabies live vaccine.
Owner:GUANGDONG WENS DAHUANONG BIOTECH +1
Recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus genetic engineering strain and application
InactiveCN101457215AAvoid infectionPrevention of swine pseudorabiesViral antigen ingredientsMicroorganism based processesAnimal virusAntigen
The invention belongs to the animal virus genetic engineering technique field, especially to a recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus gene engineering strain construction, a vaccine preparation and applications. The recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus gene engineering strain E001 lacks the pseudorabies virus main virulence gene TK, glycoprotein genes gG, gE and gI, does not express the functional glycoprotein gG and gE / gI of the pseudorabies virus; and simultaneously expresses a GP5m / M protein antigen of the porcine propagate and breath complex virus classical strain and the porcine propagate and breath complex virus internal variant GP5 protein and porcine circovirus ORF2. The strain can stimulate the porcine to produce protective immunity reaction for resisting pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus, effectively prevent the infection of the pseudorabies virus, the porcine propagate and breath complex virus and porcine circovirus. The invention also discloses a preparation and application of a tervalence genetic engineering vaccine.
Owner:HUAZHONG AGRI UNIV
Colloidal gold immunochromatographic test strip for detecting wild-type classical swine fever virus
The invention discloses a colloidal gold immunochromatographic test strip for detecting wild-type classical swine fever virus, which consists of water absorbent paper (1), a cellulose nitrate membrane (2), a colloidal gold pad (3), a sample pad (4) and a support (5), wherein the cellulose nitrate membrane contains a detection line which is formed by coating monoclonal antibody HQ06 of anti-classical swine fever virus E2 protein and a quality control line which is formed by coating rabbit anti-mouse IgG antibody; and the colloidal gold pad is combined with colloidal gold-labeled monoclonal antibody 6E10 of the anti-classical swine fever virus E2 protein. The test strip does not react with C-strain of classical swine fever virus, bovine viral diarrhea virus, porcine reproductive and respiratory syndrome virus, transmissible gastroenteritis virus, porcine epidemic diarrhea virus, porcine rotavirus, pseudorabies virus, porcine parvovirus and porcine circovirus type 2, and can accurately and sensitively identify the wild-type classical swine fever virus, thereby having good specificity, sensitivity and repeatability.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Nucleic acids of liquid-phase gene chip for synchronously detecting five porcine viruses and detection method thereof
ActiveCN104328218AHigh detection sensitivityStrong specificityMicrobiological testing/measurementMicroorganism based processesClassical swine fever virus CSFVMultiplex
The invention provides a set of nucleic acids of a liquid-phase gene chip for synchronously detecting five porcine viruses, which comprise forward and reverse primers and hybrid probes for porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), porcine pseudorabies virus (PRV), classical swine fever virus (CSFV) and porcine parvovirus (PPV). The invention also provides a multiplex liquid-phase chip high-flux molecular biology detection method of the five porcine viruses. According to the method, porcine virus nucleic acids in the sample to be detected are extracted to perform multiplex unsymmetric nucleic acid amplification / multiplex liquid-phase gene chip (suspension chip) combined detection, thereby synchronously and accurately detecting and identifying the five porcine viruses in the sample to be detected. The method has the advantages of high specificity, high sensitivity, high stability, high flux and high detection speed, and is simple to operate.
Owner:INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Colloidal gold immunochromatography test paper for antibodies of pseudorabies viruses of gE, gB and gD and preparation method
InactiveCN103792373AQuick checkMeet the needs of on-site testingBiological material analysisBiological testingAntigenColloidal au
The invention discloses a colloidal gold immunochromatography test paper for antibodies of pseudorabies viruses of gE, gB and gD and a preparation method. The test paper comprises a sample absorption area, a gold mark probe area, an immobilization antigen and antibody area, a water absorption area and a supporting plate, and the sample absorption area, the gold mark probe area, the immobilization antigen and antibody area and the water absorption area are laid on the supporting plate and are partially overlapped in sequence. The gold mark probe area is covered with gold mark probes which are mouse anti-swine IgG antibodies marked by colloidal gold. The immobilization antigen and antibody area is provided with a detection line T1 covered with pseudorabies virus gE protein, a detection line T2 covered with pseudorabies virus gB protein, a detection line T3 covered with pseudorabies virus gD protein and a control line C covered with goat anti-mouse IgG antibodies. The test paper is fast in detection, high in accuracy, strong in specificity, easy and convenient to carry and operate and capable of being used for differential diagnosis on PRV wild poisonous infection and vaccine immunity and evaluation on the PRV vaccine immunity effect at the same time.
Owner:WUHAN CHOPPER BIOLOGY
Heat resisting protective agent of bivalent live vaccine against highly pathogenic porcine reproductive and respiratory syndrome-pseudorabies and preparation method thereof
InactiveCN102727904AAvoid damageReduce loss rateViral antigen ingredientsAntiviralsSucroseHighly pathogenic
The invention discloses a heat resisting protective agent of a bivalent live vaccine against highly pathogenic porcine reproductive and respiratory syndrome-pseudorabies, and a preparation method thereof. The heat resisting protective agent of the bivalent live vaccine against highly pathogenic porcine reproductive and respiratory syndrome-pseudorabies is prepared by the following materials by weight: 0.8 to 1.2% of gelatin, 4 to 6% of soybean peptone, 0.5 to 1.5% of glycine, 3 to 5% of sucrose, 2 to 3% of trehalose, 1 to 2% of polyvinyl pyrrolidone, 0.5 to 1% of mannitol, 0.1 to 0.2% of vitamin E and the rest being injection water. The preparation method comprises steps of high-temperature sterilization, filtration sterilization through a microporous membrane and mixing. The heat resisting protective agent of the bivalent live vaccine against highly pathogenic porcine reproductive and respiratory syndrome-pseudorabies of the invention reduces damage on virus activity caused by various physical and chemical factors during the freeze drying process, provides good protection and effectively solves the problems that a conventional protective agent of live vaccine needs refrigerated transport and storage is inconvenient.
Owner:GUANGDONG DAHUANONG ANIMAL HEALTH PRODS +1
Detection reagent and method for identifying porcine pseudorabies virus vaccine strain and wild strain
InactiveCN104561374AHigh detection sensitivityGood repeatabilityMicrobiological testing/measurementDNA/RNA fragmentationDiseaseVirology
The invention provides a detection reagent and a method for identifying a porcine pseudorabies virus vaccine strains and a wild strain, and belongs to the field of animal pathogen detection. The detection reagent comprises two primer pairs as shown in SEQ ID NO.1-4 and probes as shown in SEQ ID NO.5-6. The invention further provides a method for identifying a porcine pseudorabies virus vaccine strain gD gene and a wild strain gE gene by using the detection reagent. The porcine pseudorabies virus vaccine strains and the wild strain can be simultaneously identified through dual fluorogenic quantitative PCR, high-flux detection on large-scale samples can be achieved, the detection reagent has the characteristics of rapidness, specificity, sensitivity, accuracy, simplicity and convenience, provides an effective tool for surveying porcine pseudorabies infection sources and tracing infection environments and disease sources, and has relatively good application value in prevention and control on porcine pseudorabies.
Owner:HENAN CENT FOR ANIMAL DISEASE CONTROL & PREVENTION
Heatproof lyoprotectant for live vaccine against pseudorabies and preparation method thereof, and lyophilized vaccine and preparation method thereof
ActiveCN107281481AFunction increaseImprove protectionViral antigen ingredientsAntiviralsMonosodium glutamateSucrose
The invention provides a heatproof lyoprotectant for a live vaccine against pseudorabies. The heatproof lyoprotectant comprises, by weight, 3 to 10 parts of gelatin, 1 to 5 parts of trehalose, 5 to 15 parts of sucrose, 0.1 to 2 parts bovine serum albumin, 1 to 8 parts of tryptone, 2 to 10 parts of enzyme-hydrolyzed casein, 1 to 5 parts of thiourea, 0.8 to 2 parts of L-monosodium glutamate, 0.1 to 3 parts of arginine, 0.5 to 5 parts of polyvinylpyrrolidone (PVP-K30) and 0.1 to 2 parts of mannitol. The invention also discloses a preparation method of the heatproof lyoprotectant and a lyophilized vaccine prepared from the heatproof lyoprotectant. When the heatproof lyoprotectant is used for protecting the vaccine and a specific lyophilization process is employed, lyophilization loss of viruses can be effectively reduced, the temperature tolerance of the viruses can be improved, and the vaccine can still maintain good physical properties and titer after long-term storage; i.e., the vaccine has stable characters and has the characteristics of heat resistance and long storage time.
Owner:SICHUAN HUAPAI BIO PHARMA
Preparation method of recombinant pseudorabies virus for expressing reverse neural circuit tracing of green fluorescin with high sensitivity, and application
ActiveCN106995824AImprove efficiencyRealize visualizationMicrobiological testing/measurementPreparing sample for investigationGreen fluorescent proteinViral Vaccine
The invention discloses a preparation method of a recombinant pseudorabies virus for expressing reverse neural circuit tracing of green fluorescin with high sensitivity, and application, including (1) the preparation of the recombinant pseudorabies virus for expressing green fluorescin with high sensitivity; (2) the application to marking of a neural circuit. The recombinant pseudorabies virus for expressing the green fluorescin with high sensitivity is successfully prepared by using a platform. The recombinant pseudorabies virus for expressing reverse neural circuit tracing of green fluorescin with high sensitivity is successfully obtained. Wide application value is realized in aspects of neural circuit marking, medicine screening platform building, medicine inhibition virus action mechanism, viral vaccine and diagnostic reagent invention and development, animal model building, virus replication, pathogenic mechanism analysis and the like.
Owner:衠奥生物技术(武汉)有限公司
Porcine pseudorabies virus strain and porcine pseudorabies inactivated vaccine prepared by using same
ActiveCN102344912APromote rapid proliferationHigh titerMicroorganism based processesAntiviralsPig farmsTonsil
The invention discloses a porcine pseudorabies virus strain and a porcine pseudorabies inactivated vaccine prepared by using the same. The porcine pseudorabies virus strain is separated from the brain, tonsil and other tissues of a still birth of a sow on a pig farm by virtue of subculture adaptation and has a microbial preservation number of GCMCC (China General Microbiological Culture Collection Center) No.5013. The virus strain separated in the invention has quick proliferation and high titer on ST (scheduled tribe) cells, can induce an animal to generate a high-titer neutralizing antibody, and has excellent immunogenicity. The invention also discloses a preparation method of the porcine pseudorabies inactivated vaccine, and the method comprises the following steps of: culturing the porcine pseudorabies virus strain to obtain a virus solution; adding an inactivator to inactivate the virus solution; and preparing an oil phase and a water phase, and emulsifying, thus obtaining the porcine pseudorabies inactivated vaccine. In the invention, each process parameter of the inactivated vaccine is optimized. Immune protective efficacy and safety tests prove that the inactivated vaccine has excellent immunogenicity and safety.
Owner:哈药集团生物疫苗有限公司
Porcine pseudorabies virus vaccine composition and preparation method and application thereof
ActiveCN104248757AShort timeEase of mass productionAntiviralsAntibody medical ingredientsDiseaseImmune effects
The invention provides a porcine pseudorabies virus vaccine composition. The porcine pseudorabies virus vaccine composition contains a porcine pseudorabies virus subunit antigen, or a recombinant Newcastle disease virus, namely a porcine pseudorabies virus vector. The invention further provides the preparation method and application of the vaccine composition. The vaccine composition can effectively prevent related diseases of a porcine pseudorabies virus and related diseases infected by the porcine pseudorabies virus. The combination of immunogenicity antigens in the porcine pseudorabies virus vaccine composition can be induced to generate a synergetic immune effect, thereby having good immune effect, and further lowering the immune usage amount to lower the immunization cost.
Owner:PU LIKE BIO ENG
RT-RPA (reverse transcription recombinase polymerase amplification) detection kit for fast detecting high-pathogenicity porcine reproductive and respiratory syndrome virus and application thereof
ActiveCN105567871AShorten test timeLow reaction temperatureMicrobiological testing/measurementMicroorganism based processesBiologyDifferential diagnosis
The invention discloses an RT-RPA (reverse transcription recombinase polymerase amplification) detection kit for fast detecting a high-pathogenicity porcine reproductive and respiratory syndrome virus and application thereof. The kit comprises a pair of primers and a probe, the sequences of the primers are shown as SEQ ID NO.1 and SEQ ID NO.2, and the sequence of the probe is shown as SEQ ID NO.3. It is proved through experiments that the kit can detect adverse effects of the high-pathogenicity porcine reproductive and respiratory syndrome virus (HP-PRRSV), a hog cholera virus, a C-type porcine reproductive and respiratory syndrome virus, a porcine circovirus type II, a porcine pseudorabies virus and a foot and mouth disease virus in a specificity mode. It is proved through experiments that the kit can detect out templates of at least 70 copies at the temperature of 40 DEG C on the condition of 20 min amplification, and the conformity between the kit and RT-qPCR is high. This shows that the kit can detect HP-PRRSV fast, efficiently and sensitively and provides an effective technological means for differential diagnosis of HP-PRRSV.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
ELISA (enzyme-linked immuno sorbent assay) detection kit of porcine pseudorabies virus IgM antibody
InactiveCN103308684AStrong specificityHigh sensitivityVirus peptidesFermentationIgm antibodyHorseradish peroxidase
The invention relates to an ELISA (enzyme-linked immuno sorbent assay) detection kit of a porcine pseudorabies virus IgM antibody, and a preparation method and application thereof. The ELISA detection kit of the porcine pseudorabies virus IgM antibody disclosed by the invention comprises an elisa plate of enveloping an IgM monoclonal antibody, sealing liquid, sample diluting liquid, detecting antigen, enzyme conjugate, concentrated scrubbing solution, enzyme substrate A solution, enzyme substrate B solution and stop buffer; the detecting antigen is prepared from purified porcine pseudorabies virus gE protein, structure protein gB and structure protein gD; and the enzyme conjugate is horse radish peroxidase-anti-gE, gB or gD protein enzyme compound. The specificity of the kit disclosed by the invention can be up to 100%; the sensitivity is 1:640; and the kit can be used for early diagnosis of porcine pseudorabies virus infection.
Owner:WUHAN CHOPPER BIOLOGY
Multiplex ligation-dependent probe amplification detection kit for simultaneously detecting five swine disease viruses, primers and probes
ActiveCN102943129AGuaranteed sensitivityGuaranteed specificityMicrobiological testing/measurementDNA/RNA fragmentationDiseaseMultiplex ligation-dependent probe amplification
The invention discloses a multiplex ligation-dependent probe amplification detection kit for simultaneously detecting five swine disease viruses, primers and probes. The multiplex ligation-dependent probes are shown in sequence tables SEQ ID NO:1 to SEQ ID NO:10; and the primers are shown in sequence tables SEQ ID NO:11 to SEQ ID NO:12. By using the primers, the probes and / or the multiplex ligation-dependent probe amplification detection kit containing the primers and the probes, five important swine disease pathogens such as a swine influenza virus, a swine reproductive and respiratory syndrome virus, a pseudorabies virus, a swine transmissible gastroenteritis virus and a foot-and-mouth disease virus can be simultaneously detected, thereby saving the detection time and cost and being beneficial to accurately diagnosing the epidemic diseases in time.
Owner:PEOPLES REPUBLIC OF CHINA BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Method for industrially producing pseudorabies vaccine by using bioreactor
InactiveCN102038946AHigh titerHigh degree of automation controlMicroorganism based processesAntiviralsSide effectCytopathic effect
The invention relates to a method for industrially producing a pseudorabies vaccine by using a bioreactor. The method comprises the following steps of: (1) sterilizing a microcarrier and the bioreactor, inoculating cells for vaccine production, culturing the cells, and after the cells on the microcarrier form a compact monolayer, inoculating pseudorabies virus, and continuously culturing to allow the virus to reproduce; (2) after more than 80 percent of cells are subjected to a cytopathic effect, stopping culturing, and obtaining virus liquid to prepare the pseudorabies vaccine. In the method, the pseudorabies vaccine is produced by culturing the cells in high density by the bioreactor microcarrier culture technology; and compared with the conventional roller bottle production process, the invention has the advantages that: the method has high automation control degree, saves manpower, reduces cost and is small in production land, the production can be monitored in real time, the scale is easy to expand, the produced virus has high titer and small difference among batches, and the product has stable quality and small side effect.
Owner:WUHAN CHOPPER BIOLOGY
Combined vaccines for prevention of porcine virus infections
ActiveUS20140093535A1SsRNA viruses positive-senseViral antigen ingredientsProtection sexProtective immunity
The present disclosure provides vaccine compositions comprising a PRRSV vaccine and a second porcine vaccine, which are substantially free from immuno-inhibition against each other. The second porcine virus vaccine can be CSFV and / or PRV. The preparation methods for the vaccines and the formulations are also provided. The vaccine compositions provided herein confer protective immunity to pigs against porcine reproductive and respiratory syndrome, classical swine fever, and / or pseudorabies.
Owner:SINOVET JIANGSU BIOPHARM CO LTD
Method for producing pseudorabies living vaccines by using subculture cell source and product thereof
ActiveCN101695573AImprove securityImprove immune efficiencyAntiviralsViruses/bacteriophagesPig kidneyAntibiotic Y
The invention provides a method for producing pseudorabies living vaccines by using a subculture cell source and a pseudorabies living vaccine product thereof. The method comprises the following steps: culturing pseudorabies virus low-virulent strains by using subculture cells; harvesting the strains to obtain cell culture venom; and then adding a stabilizing agent and an antibiotic into the cellculture venom, and freezing and vacuum-drying the mixture to obtain the pseudorabies living vaccines of the subculture cell source. The subculture cells are subculture cells ST of pig testicle or subculture cells PK15 or IBRS-2 of pig kidney. The method for producing the pseudorabies living vaccines by using the subculture cell source has the advantages of simple and stable production process, easy operation, high virus content, little batch difference and controllable quality, can remarkably improve the yield and quality of the vaccines and reduce the anaphylactic reaction and the like. The pseudorabies living vaccines obtained by using the production method of the invention have good safety and high immune efficacy, and have better immune protection effect on pseudorabies virulent attack.
Owner:广东永顺生物制药股份有限公司
Method for generating porcine pseudorabies virus by culturing ST cell in microcarrier of bioreactor
InactiveCN102690791AEasy to harvestReduce pollutionMicroorganism based processesAntiviralsTISSUE CULTURE INFECTIOUS DOSE 50%Volumetric Mass Density
The invention discloses a method for generating a porcine pseudorabies virus by culturing an ST cell in a microcarrier of a bioreactor. The method comprises the following steps: (1) selecting the ST cell as a cell line for making vaccine; (2), transferring and culturing the ST cell; (3) reproducing a porcine pseudorabies virus cell virus seed; (4) culturing the ST cell in the microcarrier of the bioreactor in a suspension way; (5) reproducing porcine pseudorabies virus liquid; and (6) treating the obtained virus liquid. The method has the advantages that the cell vitality is more than and equal to 90%, the living cell density is more than and equal to 1*10<7> cells / mL, and the virus liquid tissue culture infectious dose 50 (TCID50) is more than and equal to 10<8.0>. The production process is intensive and smooth, the dimension is easy to amplify, the production period is short, a small space is occupied, little pollution is caused to the environment and is easy to treat, the virus liquid is convenient to obtain, the quality is easy to balance and stabilize, the production cost can be reduced obviously, and the yield and the quality of the vaccines are improved.
Owner:哈药集团生物疫苗有限公司
Primers, probes and detection kits for detection of porcine circovirus, porcine pseudorabies virus and porcine parvovirus
ActiveCN102277455AImprove detection efficiencyMicrobiological testing/measurementFluorescence/phosphorescenceAssayViral test
The invention discloses a primer, probe and assay kit used for detecting porcine circovirus, porcine pseudorabies virus and porcine parvovirus. The primer and probe used for detecting are shown as sequences from SEQIDNO:1 to SEQIDNO:9 in a sequence table. The invention also provides an assay kit used for detecting the porcine circovirus, the porcine pseudorabies virus and porcine parvovirus. The primer and the probe which are adopted by the invention have strong specificity, and three viruses can be detected in one step, thus cost is saved, steps are reduced, and efficiency is improved. The method provided by the invention has the characteristics of high specificity, high sensitivity, high efficiency and low cost and is applicable to analysis of a larger number of samples.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Immunomagnetic bead purification kit and method for 12 kinds of swine common viruses and germs
InactiveCN105779398AEfficient separationSimple separation and purificationBacteriaMicrobiological testing/measurementBiotinStreptomycin
The invention provides an immunomagnetic bead purification kit and method for 12 kinds of swine common viruses and germs. The kit comprises a reagent A, a reagent B and a reagent C, wherein the reagent A is streptomycin immunomagnetic suspension; the reagent B is a 12-biotinylation monoclonal antibody mixed solution; and the reagent C is a 10* immunomagnetic bead separation and purification system buffer solution. The method comprises the following steps of (1) performing a pathogen suspension preparation process; (2) performing a biotin antibody preparation process; and (3) performing a pathogen immunological purification process. The immunomagnetic bead purification kit and method for 12 kinds of swine common viruses and germs provided by the invention have the advantages that 12 kinds of viruses and germs such as PPV (Porcine Parvovirus), SS-II (Streptococcus Suis Type 2), PRV (Porcine Pseudorabies Virus) and the like in a sample can be simultaneously, fast and effectively purified. The detection method has the advantages of high accuracy, high specificity, high sensitivity and high stability, and the subsequent fast diagnosis and effective detection work is facilitated.
Owner:BEIJING YISEN BIOTECH
Kit for detecting pig pseudorabies and application thereof
The invention discloses a kit for detecting pig pseudorabies. The kit comprises an assay plate coated with pig pseudorabies virus gE antigen, a rabbit anti-pig IgG antibody marked by HRP (horseradish peroxidase), fast coating buffer, fast blocking buffer, sample diluents, TMB substrate, stop buffer, 20 times wash solution, negative control, and a positive control. Through adopting pig pseudorabies virus specificity gE antigen with high purity and high activity expressed through gene engineering to coat the assay plate, and through adopting HRP-conjugate of rabbit anti-pig IgG monoclonal antibody containing HRP, the kit for detecting pig pseudorabies, provided by the invention, has the advantages of strong specificity, high sensibility, less susceptibility to misjudge of false positive or false negative and the like; moreover, the kit is simple in structure, low in detection cost, convenient and fast in operation and strong in timeliness, has a wide market prospect, and can create larger social benefits.
Owner:HENAN FENGHUA BREEDING SHARE
Heat-resisting protective agent for freeze-dried vaccine and preparing method and application thereof
ActiveCN106237339ASimple recipeEasy to manufacturePowder deliveryViral antigen ingredientsFreeze-dryingThiourea
The invention discloses a heat-resisting protective agent for freeze-dried vaccine and a preparing method and application thereof. The heat-resisting protective agent is prepared from, by mass, 0.3-0.6% of L-sodium glutamate, 8-14% of saccharose, 2-5% of D-sorbitol, 1-3% of bovine serum albumin, 0.1-0.3% of polyvinylpyrrolidone, 0.2-1% of thiourea, 3-5% of NZ-Amine AS, 0.8-1.5% of hydrolyzed gelatin and the balance PBS with PH of 7.2. The heat-resisting protective agent for freeze-dried vaccine can be applied to freeze-drying of porcine pseudorabies live vaccine and porcine reproductive and respiratory syndrome live vaccine after being subjected to asepsis treatment. By adopting the same heat-resisting protective agent and freeze-drying technology for the two viruses, freeze-drying loss is lower than 0.2 titer, virus content titer is lower than one titer after the viruses are stored at 37 DEG C for seven days, and the viruses can be stored for 24 months at 2-8 DEG C; convenience is brought for production of freeze-dried vaccine, and product quality and competitiveness are improved.
Owner:成都史纪生物制药有限公司
Pig pseudorabies virus natural low-virulent C strain and heatproof preservation method thereof
ActiveCN102399755AImprove stabilityImprove securityMicroorganism based processesViruses/bacteriophagesBiotechnologyMicroorganism
The invention relates to a pig pseudorabies virus natural low-virulent C strain and a heatproof preservation method thereof. The microbe collection registry number of the strain is CCTCC NO: V201114. According to the invention, various cryoprotectant substrates are mixed and blended according to a certain ratio; the cryoprotectants are respectively subject to aseptic processing; cryoprotectant components that can be subject to high-pressure sterilization are dissolved in bi-distilled water, and are sterilized for 15-30min under a temperature of 108-121 DEG C; cryoprotectant components that can not be subject to high-pressure sterilization are dissolved in bi-distilled water according to a certain formula, and are sterilized by using a filter membrane with a size of 0.22mum; all the cryoprotectant components are then mixed into a heatproof cryoprotectant; the heatproof cryoprotectant is mixed with a pseudorabies virus liquid according to a ratio of 1:1-1.2; and the mixture is lyophilized with a corresponding lyophilization curve. Compared to prior arts, the C strain provided by the invention has good stability. In a fifth generation animal of continuous back-procreation, the genetic sequence is stable, and no mutation is found. Therefore, the strain provided by the invention provides a good resource for the researches of pig pseudorabies low-virulent vaccines. The strain can bepreserved for 24 months under a temperature of 2-8 DEG C, and the virus titer is reduced by no more than 1 titer.
Owner:SHANGHAI CHUANG HONG BIOTECH
Fluorescence quantitative PCR kit used for detecting PRV, and application thereof
ActiveCN103509877AStrong specificityQuick checkMicrobiological testing/measurementFluorescence/phosphorescenceFluorescent reporterVaccine strain
The invention relates to a fluorescence quantitative PCR rapid detection kit used for specifically detecting pig pseudorabies virus (PRV) infection, and an application thereof. The kit comprises a specific primer pair used for amplifying PRV gE gene conserved region, and a specific TaqMan fluorescent-labeled probe. An upstream primer sequence of the specific primer pair is 5'-GAGGACGACGGGCTGTAC-3', and a downstream primer sequence of the specific primer pair is 5'-GGACATCAACAGGCGGTTG-3'. The sequence of the TaqMan probe is 5'-TGGGTCCATTCGTCACTTCCG-3'. The 5' end of the TaqMan probe is labeled with a fluorescent reporter group FAM. The 3' end of the TaqMan probe is labeled with a fluorescence quenching group BHQ1 which is not intrinsically fluorescent. The kit can be used for rapid qualitative and quantitative detections of PRV infection, and can be used in identification detections of PRV gE gene-deleted vaccine strains and / or PRV wild strains.
Owner:WUHAN CHOPPER BIOLOGY
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