96 results about "Pig kidney" patented technology

The invention discloses a mutant zymoprotein of D-amino acid oxidase and preparation method of the mutant zymoprotein. The preparation method comprises the following steps: according to homologous sequence comparison result, respectively performing mutation on 115, 119 and 286 sites of D-amino acid oxidase in primary Arthrobacterprotophormiae (DSM15035) through site-specific mutagenesis technology to construct three-site mutation expression vector Pet-e115A/N119D/T286A; and carrying out prokaryotic expression and purification to obtain zymoprotein. An apparent secondary speed constant Kcat/Km of the obtained mutant protein to substrate D-Met is 1.39*10 to the power of 5s-1.M-1, is 5.03 times of wild type DAAO (D amino acid oxidase), and is 55.96 times of pKDAAO protein of pig kidney source. The detection efficiency of partial D-amino acid can be improved, and the good application value is realized.

The invention discloses a multifunctional health-care medicament pillow for a baby. The center of the front surface and/or the middle part of a long edge of a top pillow fabric is sewed with a bottom pillow fabric to form a pillow groove which is shaped like an arc or a pig kidney to adapt to the head of the baby and has a certain slope; the bottom fabrics or the edges of a medical pillow bag and the pillow groove are provided with bag openings respectively; the bag openings are respectively sewed with a big end up fastener; the bag openings are used for disassembling and exchanging seeds of traditional Chinese medicaments and a sweat-absorbing material, adjusting the height and width of the pillow and providing convenience for disassembling and washing a pillow bag; the sweat-absorbing material with proper softness is filled in the arc or pig kidney shaped pillow groove; and one or several traditional Chinese medicaments in a form of small granular seeds of fruit of negundo, fructus cannabis and seeds of feather cockscomb are filled in the medicament pillow. The pillow has the advantages of stimulating the circulation of blood, allaying tiredness, achieving healthcare treatment, guaranteeing a well-developed baby head and comfortable sleep for the baby, improving sleep quality and strengthening body constitution of the baby, along with rational structure, good elasticity, good shape, well-chosen traditional Chinese medicaments, ventilation, dryness and comfortableness, sweat absorption, mildew resistance and sterilization.

The invention discloses ribonucleic acid interference (RNAi) for inhibiting the replication of hog cholera virus and a preparation method of RNAi. The RNAi comprises a small interfering RNA (siRNA) sequence. The preparation method comprises the steps of constructing short hairpin RNA (shRNA) slow virus expression vector, preparing replication-defective slow virus, infecting slow virus pig kidney (PK)-15 cells (pig kidney cells) by using the slow virus and the like. The invention also discloses a method for verifying the effect of inhibiting the hog cholera virus replication effect. The siRNA sequence has the obvious effect of inhibiting the replication of the hog cholera virus on sensitive cells. According to the invention, the exploration of RNA interference on in vitro and vivo replication of hog cholera virus is carried out, a slow-virtue-mediated stably-integrated RNA interfering technology for special conserved gene segments of a targeted hog cholera virus genome is constructed, and transgenic animals with the siRNA of the targeted hog cholera virus are hopeful to construct. According to the invention, necessary experimental data is accumulated for gene function research of RNAi applied to the hog cholera virtue and prevention and treatment of hog cholera, and early-stage preparation is provided for disease resistance breeding of animals.

The invention provides a method for suspension culture of subculture cells, which comprises the following steps that: (1) expression vectors containing gene sequences, capable of weakening the cell adhesion property, are used for stably transfecting pig testis cells or pig kidney cells; (2) a stable transfection cell clone is screened and separated; (3) the gene expression in the stable transfection cell clone is detected; and (4) the stable transfection cell clone is subjected to suspension culture. The method further relates to a method for producing hog cholera vaccine by using the pig testicle cells or the pig kidney cells obtained through the suspension culture.

The invention discloses a pig kidney cell line and application thereof. The preservation number of the pig kidney cell line is CCTCC (China Center for Type Culture Collection) C201324. A PK-15 cell line for stably expressing CD163 built by the pig kidney cell line can be applied to separation and cultivation of a porcine reproductive and respiratory syndrome (PRRSV) virus, and can also be applied to research of a PRRSV cell receptor. By building of a pig cell line susceptible to the PRRSV, an alternative cell line is provided for cultivation of the PRRSV; the range of a host cell for researching the PRRSV is enlarged; the pig kidney cell line has important significance on production of a PRRSV vaccine.

The invention discloses selenium-enriched health care chocolate. The selenium-enriched health care chocolate is prepared from cocoa mass, cocoa butter, konjak refined powder, galactooligosaccharide, selenium-enriched mushroom, selenium-enriched oats, selenium-enriched peanuts, tomatoes, goose eggs, duck livers, pig kidneys, lean beef, selenium-enriched yeast extract powder, selenium-enriched malt powder, salt, complex vitamins, heme iron, naringenin and dihydromyricetin. The selenium-enriched food materials and functional matter are added into a product to promote digestive absorption for selenium, and the product is antibacterial, diminishes inflammation, is resistant to oxidation and cancer, enhances immunity, promotes gastrointestinal functions and protects heart and cerebral vessels. Meat products are processed, and fishy smell is removed to enable the taste to be delicate, sweet and sour and enable the color to be ruddy. The chocolate sequentially comprises a chocolate thick layer, a goose egg powder layer, a chocolate thin layer and a meat layer from outside to inside to enable the taste to be various and the aroma to be harmonious and enable customers to have different tastes. The konjak refined powder can improve the thermal stability of the product, and the selenium-enriched health care chocolate is easy to store and transport and reduces cost.

The invention discloses an NS5B303shRNA (Short Hairclip Ribonucleic Acid) for inhibiting hog cholera virus replication. The NS5B303shRNA contains a siRNA (Small Interfering Ribonucleic Acid) sequence and is prepared through constructing a lentiviral expression vector of a shRNA, obtaining replication deficient lentivirus and lentivirus infection PK-15 cells (pig kidney cells) and verifying a hog cholera virus replication inhibiting effect. The sequence has a remarkable effect for inhibiting the replication of hop cholera viruses on sensitive cells. Through research on RNA interference to replication inside/outside the hog cholera viruses, a lentiviral vector mediated and stably-integrated RNA interfering technology for targeting a specific conserved gene fragment of a hog cholera virus genome is constructed, and siRNA transgenic animals for targeting the siRNA of hop cholera viruses are expected to be constructed. The necessary experimental data are accumulated for researching gene functions of the shRNA applied to the hop cholera viruses and preventing and treating hog cholera, and the previous preparation is provided for the animal breeding for disease resistance.

The invention discloses pig kidney soup. The pig kidney soup is composed of the following components in mass percentage: 15%-20% of pig kidneys, 2%-3% of fried eucommia ulmoides, 5%-10% of wax gourd, 10%-15% of carps, 3%-5% of coix lachryma-jobi kernels, 2%-3% of astragalus membranaceus, 3%-5% of fresh ginger, 1%-3% of salt and the balance being water. The pig kidney soup is cheap in price and good in effect, has no side effects and has the effects of tonifying the spleen and kidney.

The invention discloses a method for increasing the matrix impact resistance of ANAMMOX granules, and belongs to the field of wastewater treatment. The method comprises the following steps: carrying out impact of 1500 to 2500 mg/L of total nitrogen for 12 to 48 hours during a matrix impact period of an ANAMMOX reactor which is in stable running, and adding 0.1 mg/L to 1.5 g/L of pig kidney acylaseI into the reactor every hour until finishing the matrix impact; enabling not to add an AHLs (Acylated Homoserine Lactones) inhibitor-the pig kidney acylase I into the reactor any more after finishing the matrix impact. According to the method disclosed by the invention, the activity of an ANAMMOX granule group induction system under the matrix impact can be inhibited by adding the AHLs inhibitor-the pig kidney acylase I in lower concentration, so that the release amount of granular AHLs can be reduced, excessive release of LB-EPS (Loose Binding-Extracellular Polymeric Substance) can be controlled, and the stability and the sedimentation capacity of granules under the matrix impact can be increased; meanwhile, adverse effect to activity of the granules cannot be caused when the concentration of an inhibitor is lower.

The invention provides a Yao bath powder capable of soothing the channels and activating the collaterals. The Yao bath powder is formed by mixing, by weight, 1-2 parts of sargentodoxa cuneata, 0.2-2 parts of phryma, 0.2-2 parts of trachelospermum, 0.2-2 parts of sambucus williamsii hance, 0.2-2 parts of paederia scandens, 0.2-2 parts of sweet wormwood, 0.2-2 parts of hemistepta lyrata, 0.2-2 parts of gouania javanica, 0.2-2 parts of scandent schefflera stems and leaves and 0.2-2 parts of Hongkong rhaphidophora stems, and meanwhile pig kidney seeds and sheepear inula herb can be added. When the Yao bath powder is used, medicine juice is poured into a bathtub or a tub after the medicine powder is boiled for 3-5 min with boiled water, a proper amount of hot water is added, and people can be soaked naked when the water temperature is reduced to 35-45 DEG C. After the whole body is soaked for 5 min, the body starts to sweat and expel toxin, a person rubs and presses the body while taking a bath, the blocked channels and collaterals are smoothened through pushing, interaction and functions between bones, muscles, tendons and other organ tissue and various microcirculations are enhanced, the metabolic function is restored or enhanced, and pathological joints return to normal. The Yao bath powder has the effects of inducing sweating and expelling toxin, soothing the channels and activating blood, carrying out dehumidification and removing swelling, promoting blood circulation to remove blood stasis, expelling wind, relieving swelling and pain, eliminating toxin and expelling carbuncle, killing insects and promoting recovery of herniated lumbar disc.

The invention discloses a drug for treating fatty liver, prostatitis, diabetes, hypertension, pneumonia and gastroenteritis, wherein the drug comprises 19 Chinese herbs such as inonotuso bliquus(fr )pilat, Huidouba, rhodiola rosea l, fallopia multiflora, tall gastrodia fruit and the like. According to the present invention, the poultry eggs soused with the drug can effectively treat fatty liver, prostatitis, diabetes and hypertension; by mixing the drug liquid sousing the poultry eggs, sheep lung, pig lung and cow lung, the obtained mixture can effectively treat pneumonia, diabetes and hypertension; by mixing the drug liquid sousing the poultry eggs, sheep liver, pig liver and cow liver, the obtained mixture can effectively treat diabetes and hypertension; by mixing the drug liquid sousing the poultry eggs, sheep tripe, pig tripe and cow tripe, the obtained mixture can effectively treat gastroenteritis, diabetes and hypertension; by mixing the drug liquid sousing the poultry eggs, sheep kidney, sheep heart, pig kidney, pig heart, cow kidney and cow hear, the obtained mixture can effectively treat diabetes and hypertension; and through the technology, animal internal organs, edible salt and the drug liquid sousing duck eggs and chicken eggs and containing the effective component are mixed so as to obtain the combined drug.

The invention discloses a biscuit capable of tonifying kidney and spleen. The biscuit capable of tonifying kidney and spleen is made of raw materials of 60-70 parts by weight of peas, 55-60 parts by weight of coix lacryma-jobi, 30-40 parts by weight of lotus root starch, 1-2 parts by weight of cordyceps sinensis, 2-3 parts by weight of polygonum cuspidatum, 1-3 parts by weight of eleusine indica, 3-4 parts by weight of magnolia flower, 15-25 parts by weight of onion, 35-45 parts by weight of pig kidney, 20-30 parts by weight of soybean milk, 6-10 parts by weight of olive oil, 6-9 parts by weight of porphyra, 1-2 parts by weight of nutrition additives and water of an appropriate amount. The biscuit capable of tonifying kidney and spleen utilizes the peas, the coix lacryma-jobi and the lotus root starch as the main raw materials; the peas are sweet in taste, neutral in nature and beneficial to spleen and stomach, and has the effects of being beneficial to middle qi, stopping diarrhea, adjusting nutrient qi and defensive qi and inducing diuresis; the coix lacryma-jobi are sweet in taste and warm in nature and has the function of being beneficial to spleen and stomach, nourishing blood, being tonic and stopping diarrhea; the pig kidney contains components of zinc, ferrum, copper, phosphorus, vitamin A, B vitamins, vitamin C, protein, fat, carbohydrate and the like and has the effects of tonifying kidney, curing deficiency, engendering liquid and allaying thirst. The cordyceps sinensis is sweet in taste, warm in nature, beneficial to spleen and stomach, mild and tonic and has the effects of tonifying lung qi and being beneficial to kidney essence.

The invention discloses a compound sulfamonomethoxine sodium micro-capsule preparation and a preparation method thereof. The compound sulfamonomethoxine sodium micro-capsule preparation is prepared from sulfamonomethoxine with 8-12% of mass ratio, trimethoprim with 1-3% of mass ratio, the balance glucosum anhydricum and the balance addition agent. The preparation has the advantages that the cooperative use of 10% of sulfamonomethoxine, 2% of trimethoprim micro-capsule and addition agent is adopted, the preparation penetrating power is effectively improved, and toxoplasma cysts can be penetrated, and toxoplasmosis can be effectively prevented and controlled. Damage to the pig kidney is relieved through a micro-capsule technology, and the preparation is specially applied to the use of pregnant sows.

The invention relates to a Chinese herbal medicine for treating renal calculi. The Chinese herbal medicine for treating the renal calculi is prepared from 3-10 parts of rhizoma corydalis, 3-10 parts of curcuma aromatica, 5-15 parts of trogopterus dung, 15-30 parts of christina loosestrife herb, 6-15 parts of lygodium japonicum, 3-12 parts of endothelium corneum gigeriae galli, 9-18 parts of polygonum aviculare, 9-18 parts of pink herb, 6-12 parts of pyrrosia lingua, 9-18 parts of semen plantaginis, 10-15 parts of salviae miltiorrhizae, 6-12 parts of turtle shell, 5-15 parts of oyster, 5-15 parts of lalang grass rhizome, 5-12 parts of achyranthes root, 6-10 parts of eucommia bark and one fresh pig kidney. The invention also provides a preparation method of the Chinese herbal medicine for treating the renal calculi. The Chinese herbal medicine for treating the renal calculi has the effects of resolving stones, removing urinary calculus, replenishing renal qi, enhancing qi transformation function of urinary bladder, inducing diuresis for treating stranguria, resolving hard lumps, clearing away heat and toxic materials as well as resolving tetany and relieving pain.

The invention discloses a novel multifunctional enzyme gene HG32 and application, the enzyme is derived from Chinese rhizomyidae enteric microorganisms, can be compatible with an eukaryotic cell expression system, expresses cellulase with high biological activity, and can be applied to the fields of livestock and poultry feed enzyme preparations, transgenic animal preparation, food and medicine industries and the like; the invention solves the problems that plant cellulose is difficult to degrade, the feed nutrition utilization rate is low and the like are solved, and pollution of nitrogen, organic matter and the like in animal excrement. According to the invention, the application comprises the following steps: sequencing a bamboo rat intestinal microbe macro transcriptome, and splicing the sequences; performing homology comparison with the existing glycoside hydrolase family on NCBI, and screening high-expression suspected cellulase genes with homology higher than 50%; predicting the signal peptide, and replacing the original signal peptide with the pig parotid gland secreted protein signal peptide; optimizing according to pig codon preference, and cloning to an eukaryotic expression vector; transfecting into pig kidney epithelial cells through lipidosome for expression; collecting a cell culture supernatant; and carrying out enzyme activity analysis and comparison.

The invention discloses pericarpium citri reticulatae and ginkgo health wine which is prepared from the following raw materials in parts by weight: 250-300 parts of ginkgo, 8-10 parts of trichiurus haumela meat, 5-6 parts of nori pulp, 6-8 parts of pickled cucumber, 6-9 parts of pig kidney, 6-7 parts of celery leaf, 6-8 parts of crushed peanut, 8-9 parts of dried squid, 7-8 parts of purple corn, 8-11 parts of black garlic, 5-6 part of fine dried noodles, 5-10 parts of chili juice, 8-9 parts of chocolate powder, 2-4 parts of osbeckia sikkimensis craib, 5-7 parts of pericarpium citri reticulatae, 3-4 parts of chrysanthemum, 2-3 parts of wight anotis, 1-2 parts of alfalfa and 9-10 parts of a nutritional additive. According to the pericarpium citri reticulatae and ginkgo health wine disclosed by the invention, the ginkgo is used as a main material for brewing wine and has the functions of tonifying lung qi, treating cough and asthma and protecting blood vessels, wherein the added trichiurus haumela meat and pig kidney have the effects of tonifying deficiency, nourishing liver, nourishing yin and tonifying kidney; the purple corn taken as an auxiliary material has the effects of resisting oxidation, improving the eyesight and enhancing the immunity; meanwhile, various Chinese herbal medicines such as the pericarpium citri reticulatae are adopted, so the pericarpium citri reticulatae and ginkgo health wine disclosed by the invention has the effects of regulating the flow of qi, strengthening spleen and clearing away heat and toxic materials after being taken for a long time.

The invention discloses a medicine for treating fatty liver, prostatitis, diabetes, hypertension, cough and gastroenteritis. The medicine is prepared from multiple Chinese herbal medicines including birch antler, grifola frondosa, rhodiola rosea, polygonum multiflorum and gastrodia elata. Firstly, the medicine and eggs are pickled to effectively treat fatty liver, prostatitis, diabetes and hypertension; secondly, a liquid medicine obtained after pickling of the medicine and the eggs together with goat lungs, pig lungs and cattle lungs can effectively treat cough, hypertension and diabetes; thirdly, a liquid medicine obtained after pickling of the medicine and the eggs together with goat liver, pig liver and cattle liver can effectively treat diabetes and hypertension; fourthly, a liquid medicine obtained after pickling of the medicine and the eggs together with goat tripe, pork tripe and ox tripe can effectively treat gastroenteritis, diabetes and hypertension; and fifthly, a liquid medicine obtained after pickling of the medicine and the eggs together with goat kidney, goat heart, pig kidney, pig heart, cattle kidney and cattle heart can effectively treat diabetes and hypertension. According to the technology of the medicine, the composite medicine can be obtained after mixing liquid medicines with effective components obtained through pickling of animal viscera, salt, duck eggs and chicken eggs.

The invention discloses a selenium element extract-containing fine konjak flour health product. Raw materials of the product comprise, by weight, 20-40 parts of salmon roe sauce extract, 15-30 parts of pig kidney extract, 20-30 parts of natural squid extract, 20-50 parts of fine konjak flour, 10-35 parts of Radix Puerariae, 10-18 parts of Radix Astragali, 5-10 parts of wolfberry fruits, 2-10 parts of isatis root, 5-15 parts of honeysuckle flower, 5-15 parts of black sesame seeds, 10-30 parts of Dateplum persimmon, 5-15 parts of black beans, 3-12 parts of black fungus, 2-10 parts of black mulberry, 3-12 parts of black pine nuts, 5-10 parts of hawthorn fruits, 2-8 parts of cashews, 1-6 parts of dried longan pulp, 1-10 parts of purple sweet potato flour, 2-12 parts of egg powder, 1-5 parts of wheat germ powder, 1-5 parts of honey, 2-8 parts of citric acid, 1-5 parts of vitamin E and 2-8 parts of linoleic acid. The health product is natural, has the advantages of no artificial auxiliary materials and low cost, and is suitable for broad masses.

The invention provides a medicine for treating kidney deficiency and yang deficiency. The medicine is prepared from the following raw materials in parts by weight: 5-10 parts of chestnuts, 5-10 partsof walnuts, 5-10 parts of oysters, 20-40 parts of a pig kidney and 5-10 parts of beautiful millettia root. The medicine for treating the kidney deficiency and the yang deficiency is cheap in price, good in treatment effect and small in toxic and side effects.