4 results about "Pig kidney" patented technology

The application discloses an sgRNA capable of effectively editing a pig CCR5 gene and application thereof, and is premised on an sgRNA capable of specifically recognizing a pig CCR5 gene in a pig genome, and successfully constructs a pig fetal fibroblast cell, a pig kidney cell line PK15 cell line and a pig 3D4 / 21 cell line with EGFP site-directed integration by using CRISPR / Cas9 mediated gene knock-in technology, and the results show that the EGFP gene can be stably and efficiently expressed in the three kinds of cells or cell lines. The application provides more site selection and reference for gene function research, gene modified pig research and development.

The application provides a composite foam inhibition method for a sewage biological reaction tank and application thereof; the composite foam inhibition method for the sewage biological reaction tank comprises the following steps: S1, providing a chemical defoaming agent and an additive; the additive comprises one or more of pig kidney acylase and vanillin; S2, adding the chemical defoaming agent and the additive into sewage, and aerating the sewage. The application can not only reduce the surface tension of foam liquid film, destroy the structure of the foam liquid film, realize rapid defoaming, but also inhibit excessive proliferation of filamentous bacteria, reduce foam generation from the source, can be directly applied to foam-prone units such as activated sludge biochemical tanks and secondary sedimentation tanks in sewage plants, is simple to operate, and has significant economic benefits and good application prospect.

The invention discloses a 3D culture method of vascularized human kidney organoid and application thereof. The 3D culture method of the human kidney organoid comprises the following steps: S1, preparing a pig kidney CdECM gel solution; s2, resuspending the hPSCs by using a first differential culture medium, performing induced differentiation for 36-60 hours, and continuously culturing for 12-36 hours after half amount of the liquid is changed; replacing the culture system with a second differential culture medium, culturing for 3-7 days, and replacing half of the liquid every other day during the culture process to obtain primary human kidney organs; and S3, uniformly mixing the primary human kidney organ obtained in the step S2 and the pig kidney CdECM gel solution obtained in the step S1, standing for 10-15 minutes, transferring into a cell culture plate after gel formation, culturing for 6-8 days, and changing a half amount of the solution every other day in the period, so as to obtain the human kidney organ. According to the method, a 3D growth environment closer to the in-vivo environment is provided for kidney organ differentiation, and kidney organ blood vessel differentiation is promoted.

The application discloses a method for perfusion of an isolated pig kidney and application thereof. The method comprises using a peristaltic pump to perfuse physiological saline into the isolated pig kidney, and collecting generated urine. Compared with a static cold-preserved pig kidney, the isolated pig kidney obtained by using the perfusion method provided by the application can better maintain the integrity of a renal tubular structure according to a comparison of tissue sections after perfusion for 1 hour. The isolated pig kidney obtained by using the perfusion method provided by the application can effectively maintain the filtration function of the kidney during extracorporeal circulation.