11 results about "Pig kidney" patented technology

The utility model belongs to the technical field of pig kidney processing, and particularly relates to a pig kidney flattening and arranging mechanism which comprises supporting plates, a toothed ring is fixedly connected between the supporting plates in a matched mode, a fixing frame is fixedly connected between the top sides of the fixing plates, a connecting rod is fixedly connected in the top side of the fixing frame, and a connecting plate is movably arranged on the outer wall of the connecting rod in a sleeved mode. A supporting rod is installed in one end of the connecting plate, a driving motor is assembled on the bottom side of the supporting rod, a gear is installed at the output end of the driving motor, an inclined rod is installed on the bottom side of the gear, and a detection probe is installed at the bottom end of the inclined rod. When the pig kidney reaches a detection area, a driving motor is started, the driving motor drives a gear to rotate, the gear can move along a gear ring, then an inclined rod and a detection probe can be driven to move, and the detection probe obtains images of the pig kidney at different angles; and the control panel controls the cutter not to carry out cutting operation on the pig kidney, so that whether the pig kidney is flattened or not is detected, and the quality and the effect of follow-up cutting on the pig kidney are improved.

The application discloses an sgRNA capable of effectively editing a pig CCR5 gene and application thereof, and is premised on an sgRNA capable of specifically recognizing a pig CCR5 gene in a pig genome, and successfully constructs a pig fetal fibroblast cell, a pig kidney cell line PK15 cell line and a pig 3D4 / 21 cell line with EGFP site-directed integration by using CRISPR / Cas9 mediated gene knock-in technology, and the results show that the EGFP gene can be stably and efficiently expressed in the three kinds of cells or cell lines. The application provides more site selection and reference for gene function research, gene modified pig research and development.

The utility model discloses a pig kidney slicer which comprises a slicing workbench, the slicing workbench is rotationally connected with a rotating rod, a cutting blade is arranged above the slicing workbench, a slicing outlet groove is formed in the position, below the cutting blade, of the slicing workbench, and the bottom of the rotating rod penetrates through the inner wall of the upper portion of the slicing workbench. Rotating plates are fixedly connected to the upper portions of the rotating rods in a penetrating mode, sliding sleeves are slidably connected to the rotating plates in a penetrating mode, springs are fixedly connected to the inner walls of the upper portions of the sliding sleeves, sliding insertion rods are fixedly connected to the bottoms of the springs, and the sliding insertion rods are slidably connected to the interiors of the sliding sleeves; the tops of the five sets of sliding sleeves are fixedly connected with a sliding plate. In the cutting process of the pressing plate of the pig kidney slicer, the pressing plate cannot shift under collision of the cutting blade, meanwhile, the pressing plate can tightly press a pig kidney through the tension of the spring, wrinkles generated in the cutting process of the pig kidney are reduced, and therefore the thickness of slices obtained after the pig kidney is cut is more uniform.

The utility model discloses a slicing edge opening adjusting structure which comprises a material box, an upper opening, a lower opening and adjusting blocks arranged on the two sides. The sliding rails are symmetrically arranged on the two sides of the material box and extend in the length direction of an opening. The sliding rails are arranged on the two sides of the edge opening, the feed box is connected with the sliding blocks and the sliding rails through the adjusting blocks, the pig kidneys are placed in the feed box, the pig kidneys are close to the blades along with movement of the feed box and are cut into pieces, and the feed box is arranged on the inner side of the edge opening in a sliding mode through matching of the adjusting blocks and the sliding blocks. When downward pressure is applied to the pig kidneys from the outside, the pig kidneys can be evenly stressed, the sliced pig kidneys are better in flatness, due to the arrangement of the material box, the pig kidneys are prevented from being exposed, and the slicing safety is guaranteed.

The invention relates to the technical field of gene engineering and cell sensing, in particular to a fluorescent report cell line based on RSAD2 gene transcriptional activity detection and a preparation method thereof, which are a report strategy capable of converting the RSAD2 gene transcriptional activity into tdTomato fluorescent signals, take the transcriptional activity of an antiviral gene RSAD2 as a core, and utilize a CRISPR / Cas9 mediated gene knock-in technology to detect the RSAD2 gene transcriptional activity. A synchronous expression system of the RSAD2 gene and the tdTomato fluorescent protein is successfully constructed in a pig kidney cell line, and the LLC-PK1 cell line constructed by the report strategy can efficiently and sensitively convert the transcriptional activity of the RSAD2 gene into a tdTomato fluorescent signal, and can be widely applied to the aspects of pathogen detection, environmental monitoring, food safety, drug and poison research and the like.

The present invention relates to the technical field of slaughtering equipment, and discloses a separating device for pig kidneys and leaf lard, which includes a frame body. A roller feeding device for receiving, squeezing and conveying pig kidneys and leaf lard is provided on the frame body. Below the conveying direction of the roller feeding device, a roller pulling device for pulling the leaf lard is provided. The separating device further includes a cutting device arranged on the frame body for separating pig kidneys and leaf lard, and a power device for driving the roller feeding device and the roller pulling device to work. The pig kidneys and leaf lard are moved towards the cutting device through the roller feeding device and the roller pulling device, and the pig kidneys are extruded from the film of the leaf lard during the moving process. When the extruded pig kidneys come into contact with the cutting device, they are cut off, thereby realizing the separation of pig kidneys and leaf lard. By using the separating device to replace manual operation, the present invention can efficiently and safely separate pig kidneys and leaf lard, and reduce the damage to pig kidneys.

The utility model discloses pig kidney slicing equipment which comprises a base, and a power source is arranged at the upper end of the base; the frame body is connected with the upper end of the base; the guide assembly is connected with the frame body and the base; a conveying mechanism; the cutter assembly is arranged on the upper portion of the conveying mechanism and connected with the guide assembly. The cutter assembly is arranged on the upper portion of the conveying direction of the conveying mechanism, the guide assembly drives the cutter assembly to vertically move in the direction perpendicular to the material conveying direction, the cutter assembly is arranged on the upper portion of the conveying direction of the conveying mechanism, the guide assembly drives the cutter assembly to vertically move in a reciprocating mode, materials placed on the conveying mechanism are cut into pieces, and compared with a manual mode, the cutting efficiency is greatly improved. The mode is less influenced by artificial experience, the slicing efficiency is high, the slicing condition and thickness are uniform, and the quality is better.

The application provides a composite foam inhibition method for a sewage biological reaction tank and application thereof; the composite foam inhibition method for the sewage biological reaction tank comprises the following steps: S1, providing a chemical defoaming agent and an additive; the additive comprises one or more of pig kidney acylase and vanillin; S2, adding the chemical defoaming agent and the additive into sewage, and aerating the sewage. The application can not only reduce the surface tension of foam liquid film, destroy the structure of the foam liquid film, realize rapid defoaming, but also inhibit excessive proliferation of filamentous bacteria, reduce foam generation from the source, can be directly applied to foam-prone units such as activated sludge biochemical tanks and secondary sedimentation tanks in sewage plants, is simple to operate, and has significant economic benefits and good application prospect.

The invention discloses a 3D culture method of vascularized human kidney organoid and application thereof. The 3D culture method of the human kidney organoid comprises the following steps: S1, preparing a pig kidney CdECM gel solution; s2, resuspending the hPSCs by using a first differential culture medium, performing induced differentiation for 36-60 hours, and continuously culturing for 12-36 hours after half amount of the liquid is changed; replacing the culture system with a second differential culture medium, culturing for 3-7 days, and replacing half of the liquid every other day during the culture process to obtain primary human kidney organs; and S3, uniformly mixing the primary human kidney organ obtained in the step S2 and the pig kidney CdECM gel solution obtained in the step S1, standing for 10-15 minutes, transferring into a cell culture plate after gel formation, culturing for 6-8 days, and changing a half amount of the solution every other day in the period, so as to obtain the human kidney organ. According to the method, a 3D growth environment closer to the in-vivo environment is provided for kidney organ differentiation, and kidney organ blood vessel differentiation is promoted.

A pig waist bisection machine comprises a machine frame, a conveying mechanism, a vertical feeding mechanism and a cutter mechanism. The vertical feeding mechanism comprises a plurality of pairs of vertical feeding groups and vertical feeding power groups; the vertical feeding set comprises a pair of vertical driving rollers, a pair of inclined transmission rollers, an inclined transmission belt, a swing arm and an inclined roller frame. The swing arms of the same pair of vertical feeding groups swing and are folded at the front ends under the action of the elastic pieces; and the inclined transmission belt of each pair of vertical feeding groups forms a vertical closing opening which is gradually narrowed from a large inclined funnel opening in the direction from outside to the cutter mechanism. In the working process, when the conveying belt and the transmission belt drive the vertically and horizontally placed pig kidney to move towards the cutter mechanism from the lower portion and the two sides respectively, the pig kidney can be slowly erected till the pig kidney is completely erected before touching a cutter, and therefore the vertical pig kidney can be symmetrically cut into two halves through the cutter of the cutter mechanism; in this way, manual work is replaced to automatically complete mechanical bisection of the pig kidneys, so that the efficiency of bisection of the pig kidneys is greatly improved, and the labor intensity of workers is reduced.

The application discloses a method for perfusion of an isolated pig kidney and application thereof. The method comprises using a peristaltic pump to perfuse physiological saline into the isolated pig kidney, and collecting generated urine. Compared with a static cold-preserved pig kidney, the isolated pig kidney obtained by using the perfusion method provided by the application can better maintain the integrity of a renal tubular structure according to a comparison of tissue sections after perfusion for 1 hour. The isolated pig kidney obtained by using the perfusion method provided by the application can effectively maintain the filtration function of the kidney during extracorporeal circulation.