340 results about "Cloning" patented technology

The invention provides an SNP molecular marker combination for pop corn whole genome typing, a detection chip and application, and relates to the technical field of molecular breeding. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for genotyping of the whole genome of the pop corn, a genotyping object comprises 6419 SNP loci, the SNP markers uniformly cover the whole corn reference genome, and a high-quality molecular marker can be provided for map-based cloning of a pop corn specific agronomic trait control gene. Meanwhile, different types of corn can also be subjected to genotyping. Burst corn germplasm resources are narrower than other types of corn germplasm resources, and the chip can overcome the defect that an existing chip developed based on grain corn diversity is low in diversity in pop corn genotyping.

The invention relates to the technical field of biological medicines, and particularly discloses an anti-human CD3 rabbit recombinant monoclonal antibody as well as a preparation method and application thereof. The antibody obtains anti-CD3 immune response by immunizing a rabbit, is obtained by combining single B cell sorting, gene cloning and a mammalian cell expression system, and has high affinity and good specificity. The antibody can effectively recognize CD3 positive T cells in human peripheral blood and can be applied to the fields of flow cytometry, immunohistochemistry, T cell function research and the like. Experimental results show that the antibody has the advantages of high titer, strong fluorescence signal, low background and the like, and is superior to the traditional murine anti-CD3 antibody. Amino acid sequences of light chain and heavy chain variable regions of the antibody are further obtained, and controllability and repeatability of antibody expression are achieved. The antibody has important scientific research value and industrial application prospect, and is suitable for various scenes such as T cell marker detection, immune state evaluation, immunotherapy auxiliary research and the like.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmL424. The molecular marker is YTU024-L10; the nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; a marker primer of the molecular marker YTU024-L10 is used for carrying out PCR (Polymerase Chain Reaction) amplification on wheat genome DNA (Deoxyribose Nucleic Acid) to be detected to obtain a corresponding amplification product with the molecular weight of 238 bp, namely the molecular marker closely linked with the wheat powdery mildew resistance gene PmL424. The molecular marker provided by the invention can more accurately and efficiently detect a genetic mapping population of PmL424, and map-based cloning and fine positioning of PmL424 are facilitated; and secondly, the marker is used for molecular marker-assisted selection of PmL424, so that the wheat breeding cycle can be shortened, and the breeding efficiency can be improved.

The invention relates to a CHO-S cell strain capable of stably expressing H5N1 hemagglutinin protein and a construction method of the CHO-S cell strain, and belongs to the field of bioengineering.H5N1 hemagglutinin protein expression plasmids are obtained in an in-vitro synthesis and seamless cloning mode, 293T cells are transfected through the H5N1 hemagglutinin protein expression plasmids, and it is proved that the H5N1 hemagglutinin protein is expressed; the CHO-S cell strain capable of stably expressing the H5N1 hemagglutinin protein is obtained by transfecting CHO-S cells by using the H5N1 hemagglutinin protein expression plasmids and carrying out multiple rounds of cloning and screening, so that a basis is provided for obtaining H5N1 recombinant protein influenza vaccines.

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The invention relates to the field of plant gene map-based cloning and molecular breeding. Specifically, the invention provides a nucleic acid molecule and a polypeptide, and also provides a recombinant vector, a host cell and a plant or a part, a seed, a cell or a progeny thereof containing the nucleic acid molecule. Furthermore, the invention also provides a method for preparing the transgenic plant, and a method for regulating and controlling the nitrogen utilization efficiency, nitrate absorption or transport, biomass and / or yield of the plant.

The invention is applicable to the technical field of C-reactive protein detection, and provides an anti-C-reactive protein monoclonal antibody idiotype antibody, a preparation method and application, and the anti-C-reactive protein monoclonal antibody idiotype antibody is secreted by a hybridoma cell strain; the hybridoma cell strain is a 7C4 hybridoma cell strain; the preparation method of the anti-C-reactive protein monoclonal antibody idiotype antibody comprises the following steps: S1, obtaining hybridoma cells: preparing an anti-C-reactive protein monoclonal antibody Fab fragment, then carrying out animal immunization, then carrying out cell fusion, and finally screening and cloning the hybridoma cells; and S2, preparing and screening an anti-C reactive protein monoclonal antibody idiotype antibody. The anti-C-reactive-protein monoclonal antibody idiotype antibody is used for replacing C-reactive protein to serve as a labeled antibody, the labeling process is simple, the labeling efficiency is high, and meanwhile the situation that a final product is affected due to instability of the C-reactive protein is avoided. Experimental results show that the detection method is good in specificity and wide in linear range.

The present invention belongs to the field of biotechnology, and specifically relates to a GmSGT2 gene related to plant height and branch development, and a mutant thereof and the use thereof. A Glycine max mutant having fewer branches and decreased plant height is obtained by means of EMS mutagenesis, and a target gene thereof that is located by means of a map-based cloning technique is a GmSGT2 gene of Hedou 12. It is found through searching that the gene encodes soyasapogenol B glucuronide galactosyltransferase, and can galactosylate soyasapogenol B monoglucuronide, thereby affecting the plant height of Glycine max and reducing the branches of Glycine max. Therefore, the GmSGT2 mutant can be used for cultivating dwarf high-yield Glycine max varieties, and is of great significance in the breeding of ideal plant types of Glycine max and research on important agronomic traits of plants. The mutant has broad application prospects and great research value with regard to understanding the plant height and branch regulation mechanism of Glycine max and improving the process of Glycine max breeding; and can provide an excellent germplasm resource stock for regulating the close planting and high-yield breeding of Glycine max.

The invention relates to the technical field of plant genetic engineering, in particular to a gene of leymus secalinus participating in adversity stress and application of the gene. The gene LsHSFA participating in adversity stress in leymus secalinus is found for the first time, the nucleotide sequence of the gene LsHSFA is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO.2. Through cloning and identification of the adversity stress participating gene of the leymus secalinus, expression analysis of the gene, genetic transformation of the gene and other means, the function of the gene is analyzed and verified, and the result shows that the adversity stress participating gene of the leymus secalinus has important influence on promotion of upper development of the leymus secalinus and improvement of heat resistance, salt resistance, drought resistance and other stress resistance; the stress-resistant gene of the leymus secalinus is utilized to positively regulate and control the abiotic stress function, so that the leymus secalinus can cope with various stress adversities better, the leymus secalinus can adapt to more complex and harsh ecological environments, degenerated and desertified grassland is improved, and potential application prospects are provided for improved variety breeding and excellent population construction of the leymus secalinus in the future.

The invention relates to cloning of a stress-resistant gene LpbHLH144 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH144 of Lilium pumilum DC. Comprising the following steps: (1) cloning a target gene; (2) constructing an expression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH144 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the functions of overexpression of the LpbHLH144 for improving the saline-alkaline and drought stress of the tobacco are identified through transgenic tobacco, the functions of the lilium tenuifolium bHLH gene when the lilium tenuifolium bHLH gene is subjected to adversity stress are disclosed, a foundation is laid for further application of the lilium tenuifolium in lily breeding, and a theoretical basis is provided for the functions and effects of bHLH transcription factors.

The invention discloses application of wheat Rht1-D1b protein in regulation and control of a wheat tillering angle, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to increase the tillering angle of plants. Therefore, the invention provides the application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the tillering angle of the plant, wherein the protein can be Rht1-D1b protein; the Rht1-D1b protein can be a protein of which the amino acid sequence is as shown in SEQ ID No. 3. According to the application, the Rht1-D1b gene is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle of wheat. The wheat gene Rht1-D1b can be widely applied to the plant fields of wheat germplasm resource improvement, wheat plant type genetic breeding and the like, and has an important application value for improving the wheat yield.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination, a molecular probe combination, a liquid phase chip and a kit for corn genotyping and application of the SNP molecular marker combination, the molecular probe combination, the liquid phase chip and the kit, and belongs to the technical field of gene chips. The SNP molecular marker combination and the liquid chip provided by the invention can be used for efficient and rapid genetic typing of corn germplasm resources, and are low in cost and suitable for corn biological breeding. The SNP marker provided by the invention uniformly covers the whole corn reference genome, provides a high-quality molecular marker for silage corn whole genome selective breeding, and can also be used for map-based cloning of important agronomic trait genes of silage corn.

The invention discloses an SSR (simple sequence repeat) molecular marker associated with fresh weight character of corydalis tuber bulbules as well as primers and application of the SSR molecular marker, and belongs to the technical field of molecular genetics. According to the SSR molecular marker associated with the fresh weight character of the corydalis tuber bulbous disclosed by the invention, the amplification primer sequences of the SSR molecular marker are shown as SEQ ID NO.1 and SEQ ID NO.2. The SSR molecular marker associated with the fresh weight character of the rhizoma corydalis coleobulb and the amplification primer thereof can be applied to rhizoma corydalis population structure analysis, rhizoma corydalis coleobulb fresh weight character associated marker analysis, rhizoma corydalis excellent germplasm breeding and preparation of a kit for screening rhizoma corydalis excellent germplasm with high coleobulb weight. The method can be directly used for excellent germplasm screening, gene localization and cloning and molecular marker-assisted breeding, has chromosome localization information, is beneficial to further fine localization and cloning of genes for controlling fresh weight characters of corpus balls, and has important significance in breeding of high-yield rhizoma corydalis.

The invention discloses a tea tree CsBZR1 gene and cold resistance application thereof, the tea tree CsBZR1 gene is a core gene in a tea tree brassinolide signal transduction pathway, and the nucleotide sequence of the tea tree CsBZR1 gene is shown as SEQ ID NO.1 in a sequence table. According to the application of the CsBZR1 gene of the tea tree to regulation and control of cold resistance of the tea tree, the low-temperature tolerance of the tea tree can be reduced by inhibiting expression of the CsBZR1 gene in vivo, and the cold resistance of arabidopsis thaliana is improved by overexpression of the CsBZR1 gene. Expression of CsBZR1 of a tea tree under a low-temperature condition is directly induced to be up-regulated by low-temperature stress of the tea tree, cold resistance of arabidopsis thaliana is improved by overexpression of the gene in the arabidopsis thaliana, and low-temperature damage of the tea tree can be remarkably aggravated by inhibiting expression of CsBZR1 in the tea tree through an antisense oligonucleotide technology. The cloning of the gene not only is beneficial to determining the effects of brassinolide and the signal transduction core factor BZR1 thereof in the cold-resistant process of the tea tree, but also is beneficial to promoting the genetic improvement process aiming at enhancing the cold resistance of the tea tree.

The invention provides helper nucleic acid enhanced seamless cloning. The present invention provides products, methods and uses related to helper nucleic acid enhanced seamless cloning.

The invention discloses a method for quickly rescuing bovine coronavirus epidemic strains and application of the method. The bovine coronavirus SHZ isolate is subjected to efficient segmented cloning, the obtained segment and a Linker sequence are subjected to a cyclic polymerase extension reaction, and the obtained reaction product can be directly transfected to 293T cells for virus packaging without purification. Different from a traditional method, the method does not need to amplify full-length virus cDNA clone by means of bacteria and yeast, but directly obtains a sufficient amount of preliminary products through PCR, and avoids the problems of instability and low efficiency when partial sequences of a virus genome proliferate in a bacteria or yeast host. By adopting the reverse genetic system, the recombinant bovine coronavirus expressing the foreign protein is successfully rescued, a brand new technical platform is provided for dynamic visualization research of in-vivo and in-vitro replication of the virus, and an efficient and flexible tool is also provided for virology research, development of virus vector vaccines and screening of antiviral drugs.

The invention discloses a rice seed dormancy gene OsPHS1 and application of a protein encoded by the gene OsPHS1, and belongs to the technical field of plant genetic engineering, and the gene and the protein encoded by the gene are used for regulating the dormancy of rice seeds. The nucleotide sequence of the gene is shown as Seq ID No: 1, the CDS sequence is shown as Seq ID No: 2, and the coded protein sequence is shown as Seq ID No: 3. The function of the rice seed dormancy gene OsPHS1 is verified by cloning and identifying the rice seed dormancy gene OsPHS1, and research finds that the dormancy of rice seeds can be remarkably reduced by knocking out or mutating the OsPHS1 gene, and the dormancy of the rice seeds can be improved to a certain extent by overexpressing the gene; the invention plays an important role in developing rice varieties with moderate dormancy.

The invention relates to a recombinant expression cat interferon-omega gene and a preparation method and application thereof, the nucleotide sequence of the cat interferon-omega gene is as shown in SEQ ID NO.1, and the cat interferon-omega gene is obtained by introducing an Fc fusion fragment to the C terminal and / or N terminal of the natural gene sequence of the cat interferon-omega. XTEN or PAS is introduced to prolong a peptide fragment, and original glycosylation modification sites on a natural sequence are reserved. The method comprises the following steps: cloning a cat interferon-omega gene into an expression vector containing double promoters, further transfecting into a cell, carrying out stable cloning and screening, establishing a high-expression cell strain, and carrying out fermentation culture in a bioreactor. According to the invention, the cat interferon-omega gene is subjected to multiple modification and is efficiently expressed in a CHO-K1GS system, so that the protein yield, the stability and the half-life period are remarkably improved; the obtained fusion protein is high in purity and strong in activity, can obviously enhance immune response and protection effect when being matched with cat vaccines, and is good in safety.

The invention discloses a molecular marker closely linked with a wheat powdery mildew resistance gene PmCWI16926. The molecular marker is YTU2BS-P12, and the molecular marker is a molecular marker PmCWI16926. The nucleotide sequence of an upstream primer of the molecular marker is as shown in SEQ ID NO: 1; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2; the molecular marker is used for carrying out PCR (Polymerase Chain Reaction) amplification on to-be-detected wheat genome DNA (Deoxyribose Nucleic Acid) to obtain a corresponding amplification product with the molecular weight According to the molecular marker YTU2BS-P12 closely linked with the wheat powdery mildew resistance gene PmCWI16926, provided by the invention, a genetic mapping group of the PmCWI16926 can be more efficiently detected, and map-based cloning of the PmCWI16926 is facilitated; when the marker is used for molecular marker-assisted selection of PmCWI16926, the breeding period can be shortened, the breeding efficiency can be improved, and the marker can be better applied to wheat breeding for disease resistance.

The invention relates to the field of plant gene map-based cloning and molecular breeding. Specifically, the invention provides a nucleic acid molecule and a polypeptide, and also provides a recombinant vector, a host cell and a plant or a part, a seed, a cell or a progeny thereof containing the nucleic acid molecule. Furthermore, the invention also provides a method for preparing the transgenic plant, and a method for regulating and controlling the nitrogen utilization efficiency, nitrate absorption or transport, biomass and / or yield of the plant.

Provided are a fully human TSH receptor (TSHR) blocking monoclonal antibody for treating hyperthyroidism, and a preparation method therefor and a use thereof. The method comprises the following steps: using flow cytometry to sort plasma cells and memory single B cells specifically recognizing a TSHR in the peripheral blood of patients having high titers of TSHR blocking antibody (TSH-stimulating blocking antibody, TBAb), performing in vitro cloning of antibody light and heavy chains and performing recombinant expression, and using hTSHR-CHO cells for antibody property screening and validation, to obtain a blocking monoclonal antibody specifically targeting human TSHR. The fully human TSHR blocking monoclonal antibody can specifically bind to the TSHR, has good inhibitory activity in vitro and in vivo experiments, and has broad application prospects in the development of treatment of Graves' diseases.

The invention discloses a method for preparing bovine MSTN gene editing cells by using CRISPR / Cas12i, and belongs to the technical field of biology. According to the invention, an sgRNA expression vector aiming at the bovine MSTN gene is constructed, and a CRISPR / Cas12i system is utilized to carry out precise gene editing on a specific target area of a first exon of the bovine primary fibroblast MSTN gene, so that mRNA of the MSTN gene cannot be normally transcribed, and MSTN gene deleted cells are obtained. Compared with other gene editing tools, the Cas12i system shows higher specificity and editing efficiency (improved by 3%-5%), and the off-target effect risk is effectively reduced. The prepared MSTN gene deleted bovine recombinant cell line is stable and efficient in editing efficiency, the cell expression level is not affected, the MSTN gene deleted bovine recombinant cell line can be widely applied to somatic cell cloning and microinjection preparation of double-muscle-hip beef cattle, and a new strategy is provided for beef cattle breeding.

The present invention discloses an enterovirus infectious clone with a novel HiBiT reporter gene and a construction method and application thereof, including enterovirus EVA71, CVA16, CVA10 and CVA7 infectious clones with a novel HiBiT reporter gene, using HiBiT as a reporter gene for the first time on the basis of EVA71, CVA16, CVA7 and CVA10 infectious clones, preparing EVA71-HiBiT, CVA16-HiBiT, CVA7-HiBiT and CVA10-HiBiT infectious clones, further rescuing the reporter virus by the infectious clone, and verifying the biological activity and genetic stability of the reporter virus by cytopathic effect, plaque, WB and flow cytometry in vitro. The virus clone or reporter virus involved in the present invention has a wide range of application value in the field of anti-enterovirus drugs and high-throughput screening of neutralizing antibodies.

The invention relates to the field of cell biology, and particularly discloses a neural stem cell induced differentiation culture medium and application thereof in preparation of neural stem cells. The neural stem cell induced differentiation culture medium is composed of a basic culture medium and additives, wherein the additives are composed of an N-2 additive, a B-27 additive, glutamine, a human leukemia inhibiting factor, a GSK-3 inhibitor, an ALK inhibitor, a gamma-secretase inhibitor and a CDK8 inhibitor. The neural stem cell induced differentiation culture medium is clear and simple in component, does not need to use antibiotics, and does not contain animal-derived components. When the culture medium is used for carrying out differentiation induction on the human pluripotent stem cells, the human pluripotent stem cells can be induced and differentiated into the neural stem cells within a short time (6-7 days) without depending on pluripotent stem cell cloning and a single cell state, the nonuniformity of pluripotent stem cell cloning formation can be overcome, and the purity of the neural stem cells is improved.

The invention discloses a rape waterlogging-resistant gene BnA07ERF151 and an application thereof. The nucleotide sequence of the gene BnA07ERF151 is as shown in SEQ ID NO. 1. The invention also discloses a method for preparing the rape waterlogging-resistant gene BnA07ERF151. According to the invention, a brand new stain-resistant gene BnA07ERF151 is cloned from rape for the first time, and is transferred into arabidopsis thaliana to construct a pure line plant and complete stain resistance identification. Experimental results show that the growth state of a transgenic BnA07ERF151 gene strain in a waterlogging environment is remarkably superior to that of a wild type, bolting development is normal, the survival rate is increased to 43.75% from 12.5%, and the key effect of the gene on enhancing the waterlogging resistance of plants is clearly confirmed; successful cloning of the waterlogging-resistant gene provides a core gene resource for cultivation of new crop varieties, a variety of crops can be endowed with stronger waterlogging tolerance through a transgenic assisted breeding means, and the yield stability under extreme climate is effectively guaranteed, which has great strategic significance in improvement of global agricultural disaster resistance and guarantee of grain safety, and has a wide application prospect. And the method has remarkable social and economic benefits and industrial application prospects.

The invention relates to a key gene CbLTP63 for regulating and controlling salt stress response of catalpa bungei and application of the key gene CbLTP63, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the CbLTP63 gene is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a catalpa bungei salt stress response key gene CbLTP63. Identifying that the CbLTP63-GFP subcell is positioned on a cell membrane through an agrobacterium injection method; through qRT-PCR analysis and identification, the gene responds to salt stress on the transcriptional level and is down-regulated by H2O2, ABA and SA; through transgenosis identification, the gene is a catalpa bungei salt stress response negative regulation gene, and the salt tolerance of catalpa bungei calluses after gene silencing is obviously enhanced; the invention also relates to utilization of the gene in regulating stress response of catalpa bungei. The invention discloses a catalpa bungei salt stress response key gene CbLTP63, which has important application value in the fields of catalpa bungei gene engineering and clonal forestry.

The invention discloses a method for inhibiting tomato brown wrinkled fruit virus by using beta-glucan and application. The method comprises the following steps: spraying a prepared beta-glucan solution with the concentration of 25mg / L to 100mg / L to Bensi tobacco leaves; after 48 hours after the beta-glucan solution is sprayed, agrobacterium tumefaciens obtained through ToBRFV-GFP infectious cloning is used for inoculation to the lower leaves of the nicotiana benthamiana; 11 days after virus inoculation, the virus amount of ToBRFV is reduced, and the fluorescence intensity of GFP is weakened.

Provided is a high-throughput microbe smart cloning and information analysis device, comprising a machine body, a strain feeding mechanism, a petri dish feeding mechanism, a lid opening mechanism, a lid opening and photographing mechanism, an inoculation mechanism, an inoculation needle replacement mechanism, a liquid level detection system, a pressure detection system, a high-performance computing module, and a control management system. The lid opening mechanism and the lid opening and photographing mechanism open the upper lids of strains and empty petri dishes that are transported to an inoculation position by the strain feeding mechanism and the petri dish feeding mechanism, respectively, and photograph the strains; the high-performance computing module receives image information, and locates and identifies the strains by means of a target detection algorithm; the inoculation mechanism moves an inoculation needle to inoculate the strains, while the liquid level detection system and the pressure detection system prevent the inoculation needle from pressing or damaging the petri dishes, and the inoculation needle replacement mechanism is used to replace the inoculation needle. By means of an automated device replacing manual operations to implement strain cloning actions, inoculation efficiency is improved, the safety of operators and strains is assured, and it is possible record and analyze strain information.

The invention discloses a corn kernel development regulation gene ZmRH51 as well as an encoding protein, a functional marker and an application of the corn kernel development regulation gene ZmRH51. According to the application, a corn kernel mutant m223 is taken as a basic material, heterozygote of the corn kernel mutant m223 is hybridized with Zheng 58 (Z58) to construct an F2 segregation population, then phenotype identification and cytological observation are performed on the segregation population of the offspring, the gene ZmRH51 for controlling the mutation phenotype of the m223 is obtained through genetic analysis, BSR-seq analysis and map-based cloning, the gene is positioned in a nucleolus region, and the mutation phenotype of the m223 is controlled. The ZmRH51 is used for coding RNA helicase of a DEAD-Box gene family, and research shows that ZmRH51 participates in ribosome assembly and can influence processing of pre-rRNA in corn kernels; the excellent allelic variation genotype of the excavated ZmRH51 gene in the aspect of grain thickness is obtained through further excavation, the grain thickness and hundred-grain weight of the ZmRH51 gene can be increased through the utilization of the gene, and then the yield is increased. In addition, distribution and utilization potentials of different haplotypes in a selfing line are researched, and technical support and excellent germplasm resources are provided for utilization of the gene.

The invention discloses application of a wheat TaCKS1 protein, a coding gene and a mutant sequence of the wheat TaCKS1 protein in regulation and control of wheat grain development, and belongs to the technical field of biology, and the amino acid sequence of the TaCKS1 protein is as shown in SEQ ID NO.7. According to the invention, an SUC1 / CKS1 homologous gene TaCKS1 is obtained from homologous cloning of arabidopsis thaliana into wheat, a tacks1 mutant is screened from Jimai 20EMS mutagenesis population, and analysis on grain phenotypes of the tacks1 mutant and a wild type JM20 shows that the grain length, the grain width, the thousand grain weight and the single plant yield of the mutant are increased compared with those of the wild type JM20. The TaCKS1 gene of wheat is identified for the first time, the function of the TaCKS1 gene for negatively regulating the grain length, grain width, thousand grain weight and single plant yield of wheat is proved through EMS mutation experience, and a new gene resource is provided for wheat breeding.