3527 results about "Bacteria" patented technology

The invention discloses an aspergillus niger recombinant bacterium AnCT-XynB-K-agdA and application thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, Aspergillus niger AnCat is taken as an expression host, firstly, an acid protease regulatory factor prtT is knocked out to obtain a defective strain, and then different chaperonins are subjected to fusion expression by optimizing integration sites of a laccase lcc9 expression cassette, so that the high-expression laccase chaperonin is obtained. According to the method, an expression vector pC3-5 'agdA-PcitA-XynB-L-lcc9-hph-3' agdA is constructed, a recombinant bacterium AnCT-XynB-K-agdA is further obtained, the enzyme activity of the extracellular laccase subjected to shake flask fermentation reaches 1821.2 U / L, and finally, the recombinant efficient expression of the laccase Lcc9 in aspergillus niger is realized.

The invention relates to the technical field of ecological restoration, in particular to a preparation method of novel vegetation concrete greening additive AB bacteria, which comprises the following steps: step (1) screening and activating double bacteria; (2) preparing an inner core structure; (3) loading double bacteria; (4) coating a shell layer; (5) loading a functional matrix; and (6) preparing the vegetation concrete greening additive AB bacteria. According to the method, a multi-layer and function-synergistic microorganism-seed-carrier composite system is constructed and used for surface concrete attachment of the high steep slope, and a plant-microorganism symbiotic system with physical isolation, chemical buffering and nutrition self-sustaining capacities is actively constructed for plant seeds and functional microorganisms in a strongly alkaline macroscopic environment; and finally, the long-acting ecological stability of the high and steep slope is realized.

The invention discloses a dual-mechanism antibacterial photosensitizer as well as a preparation method and application thereof, and relates to the technical field of biomedical photoelectric functional materials. The invention relates to a triphenylamine derivative, which can realize fluorescence imaging of bacterial or fungal infection by utilizing fluorescence characteristics, can generate active oxygen under illumination to play a photodynamic therapy role, is suitable for preparing photodynamic therapy preparations and developing antibacterial materials, and can also be applied to the fields of environment and public health and tumor treatment. The problems that an existing photosensitizer is insufficient in broad spectrum, different in fungus drug resistance mechanism, limited in single treatment effect and the like can be effectively solved, and the photosensitizer has wide application prospects.

The present application relates to the field of municipal wastewater treatment, and particularly relates to a highway service area wastewater treatment device and method based on AOOA-MBBR process. The device comprises a first anoxic tank, a first aerobic tank, a second aerobic tank and a second anoxic tank connected in sequence, and is filled with MBBR filler respectively, and the volume of the filled MBBR filler is taken as the effective volume; the first aerobic tank and the second aerobic tank are respectively provided with an aeration device; the first anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the first aerobic tank is used for inoculating hydrolytic bacteria, nitrifying bacteria and denitrifying bacteria; the second aerobic tank is used for inoculating nitrifying bacteria, and the second anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the second aerobic tank is connected with the first anoxic tank through a reflux pipe, and the reflux pipe is provided with a reflux power assembly. The present application solves the problems of existing highway service wastewater treatment with extremely low carbon-nitrogen ratio, such as the need for additional carbon source / electron donor, high operation cost, poor denitrification effect and high sludge yield.

The invention discloses an identification and analysis method for unknown pathogenic microorganisms, which comprises the following steps: filtering out genome sequences with low integrity, pollution and tag errors, and establishing a high-quality virus identification database; constructing a virus host prediction model through a machine learning algorithm; unknown pathogenic microorganisms are identified and analyzed, potential hosts or pathogenicity of the unknown microorganisms are identified, and whether the unknown microorganisms are unknown pathogenic viruses or bacteria or not is further judged. On the basis of metagenome data analysis, potential unknown pathogenic microorganisms in samples of human bodies, environments and the like can be identified more accurately.

The invention discloses three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms, dsRNA and application thereof. Specifically, the nucleotide sequences of the three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms are respectively SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The recombinant Hc beta GRP protein is successfully obtained through a prokaryotic expression system, an in-vitro bacteriostatic test proves that the recombinant Hc beta GRP protein, the Hc beta GRP protein and the dsRNA have direct bacteriostatic activity, dsRNA is designed based on a pattern recognition protein Hc beta GRP gene, a hyphantria cunea Hc beta GRP dsRNA interference system implemented by using an injection method is established, an obvious gene silencing effect is obtained by injecting the Hc beta GRP dsRNA interference system into hyphantria cunea, and the Hc beta GRP dsRNA interference system is applied to the hyphantria cunea. The technical support is provided for the application of the Hc beta GRP RNAi technology. Besides, the dsRNA of the hyphantria cunea Hc beta GRP is combined with the biocontrol bacteria by utilizing an established hyphantria cunea Hc beta GRP RNA interference system, so that the control effect of the biocontrol bacteria can be remarkably improved.

The utility model discloses a cleaning device for stainless steel tableware production, which relates to the technical field of stainless steel tableware production and comprises a shell, a central controller is fixedly connected to the right side of the front side of the shell, a top frame is fixedly connected to the left side of the top of a fixing frame, and all-directional washing components are arranged in the shell and the fixing frame. A drying assembly is arranged in the top frame, and a filter screen cleaning assembly is arranged at the top of the filter screen. According to the stainless steel tableware washing device, the side faces, the top and the bottom of tableware can be washed in a water spraying mode from multiple angles through the arranged all-dimensional washing assembly, the problem that part of stains are left due to washing in a single direction is solved, and meanwhile the efficiency of the cleaning link in the whole stainless steel tableware production process is improved; and residues accumulated on the surface of the filter screen can be scraped off in time, meshes of the filter screen are kept unobstructed, tableware can be dried in time through the arranged drying assembly, and breeding of microorganisms such as bacteria is effectively inhibited.

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention discloses L-arabinose isomerase, engineering bacteria and application, the amino acid sequence of the L-arabinose isomerase is shown as SEQ ID NO: 3, and the gene sequence for coding the L-arabinose isomerase is shown as SEQ ID NO: 2. On the basis of a genome of a Peribacillussp.S4 strain, a gene for coding the L-arabinose isomerase is excavated, and a stable prokaryotic expression system is constructed by performing codon optimization on the gene, so that the large-scale controllable production of the L-arabinose isomerase is realized, the yield of the enzyme is remarkably increased, the production cost of the enzyme is reduced, and the method is suitable for industrial production. A stable and economical enzyme source is provided for the production of D-tagatose; the L-arabinose isomerase expressed by the constructed engineering bacteria can significantly improve the yield of D-tagatose, and industrialization of D-tagatose is facilitated.

The invention discloses Mucor abundans and application of the Mucor abundans, the strain number of the Mucor abundans is JSAFC 2484, the Mucor abundans is preserved in the China General Microbiological Culture Collection Center on May 6, 2025, and the preservation number is CGMCC No. 41979. The Mucor abundans can be applied to the field of biological engineering. The biocontrol bacterium Mucor abundans JSAFC 2484 provided by the invention can effectively inhibit the growth of Fusarium equiseti, the inhibition rate of plate confrontation culture reaches 68.52%, meanwhile, extracellular metabolites secreted by the biocontrol bacterium Mucor abundans JSAFC 2484 can also significantly reduce the growth of the Fusarium equiseti, when the concentration of the extracellular metabolites of the JSAFC 2484 is 10 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 76.35%, and when the concentration of the extracellular metabolites of the JSAFC 2484 is 100 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 79.05%. Meanwhile, the plant growth promoting effect is achieved on pepper plants.

According to the device and the reagent for extracting the pathogen, the pathogen product separated from the uncultured sample extracted by using the device and the reagent can be subjected to a drug sensitivity test after simple culture and bacteria collection treatment, so that the culture process is shortened compared with pathogen diagnosis and identification of a clinical infected sample, and a drug sensitivity result can be obtained more quickly; and a more timely diagnosis and treatment reference is provided for clinicians.

The invention discloses a culture tail water resource treatment system based on pearl mussels, which belongs to the technical field of aquaculture and water environment protection and comprises a culture pond, a plurality of first treatment ponds, at least one second treatment pond, a third pipeline and a control unit. Each first treatment pond is connected with the culture pond through a first pipeline, activated sludge is laid in each first treatment pond, and an aeration device and an adsorption filter with the height capable of being adjusted through a lifting mechanism are arranged on each first treatment pond. The second treatment pond is connected with the first treatment pond through a second pipeline, the interior of the second treatment pond is divided into a phycomycete symbiotic area and a water quality purification area, the water quality purification area sequentially comprises a supporting layer, an adsorption layer and a substrate layer from bottom to top, and various aquatic plants are planted in the substrate layer. The third pipeline is connected with the second treatment pond and the culture pond to form a closed water treatment circulation loop. The control unit is responsible for monitoring and regulating the operation state of the system. According to the system, efficient purification and resource recovery of aquaculture tail water are realized through multi-stage treatment and cyclic utilization.

The invention relates to a metallized microbubble and a preparation method thereof, a construction method of a multifunctional composite probe, and a rapid bacterium capturing and sterilizing method, and belongs to the technical field of functional material preparation. The method aims at solving the technical problems that in the prior art, a metal coating on the surface of the hollow glass microbubble is not uniform, combination is not firm, and stability is poor. According to the technical scheme, the method is characterized by comprising the following steps: mixing surface-activated hollow glass microbubbles with a polyethyleneimine solution for reaction, and washing and drying to obtain polyethyleneimine-coated hollow glass microbubbles; then reacting with a single-stranded DNA buffer solution to obtain an intermediate product loaded with DNA; then introducing a silver nitrate solution to load silver ions on the surface of the compound; and finally, reducing through a sodium borohydride solution to prepare the metallized microbubbles. The metalized microbubbles prepared by the method can be used for constructing a multifunctional composite probe to realize efficient capture and synergistic antibiosis of targets such as bacteria.

A pathogen nucleic acid aerosol purification device comprises a machine shell, the machine shell comprises a frame, a front panel, a left side plate, a right side plate, a rear baffle and a top cover plate are arranged on the frame, the front panel is an arc-shaped plate with the plate face bent forwards gradually from bottom to top, the rear baffle and the top cover plate are rectangular, and an air inlet is formed in the bottom of the machine shell. The front panel, the left side plate, the right side plate, the rear baffle and the top cover plate define an air channel allowing air to circulate from bottom to top, and a plurality of centrifugal fans are arranged on the upper portion in the air channel side by side at intervals in the left-right horizontal direction. The utility model aims to provide a biological laboratory air purification device which can enable air in a biological laboratory to fully circulate without dead angles, enables the air to be free of eddy current and cross disturbance as far as possible during circulation, and can efficiently disinfect and remove nucleic acid fragments, viruses and bacterial microorganisms in aerosol in the air in the biological laboratory in an extremely short time. And the pathogen nucleic acid aerosol purification device is used for efficiently removing suspended particulate matters in air in a biological laboratory.

The utility model provides an algal-bacterial symbiosis synergistic denitrification reaction device based on anaerobic ammonia oxidation, and relates to the technical field of wastewater treatment. The device comprises a reactor and a plurality of biological ribbons arranged in the reactor, a water inlet and a water outlet are formed in the two ends of the reactor respectively, the biological ribbons are vertically distributed in the reactor in the water flow direction and divide the reactor into a plurality of reaction spaces, and the two ends of the tops of the biological ribbons are fixed to the side wall of the reactor; and the height of the biological ribbon is lower than that of the reactor. The anaerobic ammonia oxidation-phycomycete symbiotic system is introduced into the reactor containing the biological ribbons, low-energy-consumption and low-emission treatment of low-C / N urban domestic sewage is achieved, the biological ribbons are selected as a loading material of the reactor, the interior of the reactor is provided with rich pore structures, and the biological ribbons are loaded on the reactor. A large amount of biological attachment surface area is provided for growth and reproduction of algae and other microorganisms in water, and a large amount of attachment sites and powerful growth conditions are provided, so that the sewage treatment effect is improved.

The invention belongs to the technical field of biosynthesis, and particularly relates to an engineering bacterium for preparing stevioside through de novo fermentation as well as a preparation method and application of the engineering bacterium. According to the invention, mevalonate kinase in the MVA pathway is mutated and optimized. The method comprises the following steps: carrying out point mutation on mevalonate kinase MvK of a wild type source, constructing a stevioside synthesis route in an escherichia coli host, and comparing the influence of MvK mutation on the yield of stevioside, so as to determine a Q160L mutant, and applying the Q160L mutant to the stevioside production route to realize efficient production of stevioside.

This invention relates to microorganisms that produce L-glutamic acid and a method for producing amino acids. The method involves culturing microorganisms in a culture medium that have the ability to produce one or more amino acids selected from L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline, and L-arginine, and that have been modified to increase the activity of α-ketoglutarate synthase, thereby generating and accumulating the L-amino acids in the culture medium or in the cells, and collecting the L-amino acids from the culture medium or in the cells.

The invention relates to the technical field of fertilizers and pesticides for agricultural production, and discloses a liquid pesticide fertilizer for preventing and treating root-knot nematodes in a water-fertilizer integrated system and application thereof, and the liquid pesticide fertilizer is prepared from the following components: a plant source nematicide, a biocontrol inoculant, a macroelement and medium trace element fertilizer, a boron cross-linking agent, a modified polycarboxylic acid dispersing agent, a microbial protective agent and the like. The preparation method comprises the steps of biological phase pretreatment, matrix phase construction, oil phase micro-emulsification, multiphase fusion and dynamic crosslinking. Through trehalose gradient permeation pretreatment, the survival rate of biocontrol bacteria in a high-salt matrix is increased; a boric acid ester bond dynamic cross-linked network is constructed by using a variable pH adjusting process, so that the system is endowed with shear thinning characteristics, and the contradiction between suspension stability and drip irrigation fluidity is solved; and the chelating dispersion effect of the modified polycarboxylic acid dispersing agent is matched, so that the generation of precipitates is effectively inhibited, and stable coexistence and synergistic interaction of the pesticide, the fertilizer and the bacteria are realized.

The application discloses a method for efficient denitrification and decarburization of kitchen waste and sludge co-fermentation anaerobic digestion effluent, which removes part of COD and ammonia nitrogen in the influent sludge by using a first SNAD, and maintains a certain amount of free ammonia of a certain concentration due to the high concentration of ammonia nitrogen and the high pH of the influent, so as to maintain the inhibition effect on nitrifying bacteria and ensure the conversion of part of the ammonia nitrogen into nitrite nitrogen, and the high activity of anaerobic ammonia oxidation bacteria is ensured in the first SNAD by limiting the aeration amount, so that the ammonia nitrogen and nitrite can be further removed; the effluent enters a nitrification tank, and the ammonia nitrogen is nitrified into nitrate nitrogen under the condition of nitrifying bacteria and sufficient aeration amount, and then enters a denitrification tank, and the denitrification process is carried out under the condition of heterotrophic denitrifying bacteria and sufficient carbon source, so that most of the nitrate nitrogen in the system is converted into nitrogen gas and removed, finally, the clear liquid is discharged after being filtered by an MBR membrane in a membrane tank, so as to achieve the purpose of efficient denitrification and decarburization of kitchen waste anaerobic digestion effluent.

The invention relates to the technical field of microbiological detection and image detection, and provides a microwell plate microbiological growth state detection method, system and equipment based on artificial intelligence. The system comprises an image acquisition module, an image segmentation module, a data set construction module, an image data enhancement module, an image classification module, a classification result determination module and a reasoning module, and is used for detecting whether bacteria grow in the microwell plate; or the system comprises an image regression module and a regression error evaluation module and is used for detecting the biomass of the thalli. According to the invention, a self-built photographing device can be adopted to realize on-site direct automatic detection without sampling, so that high-throughput automatic accurate analysis of the growth state of microorganisms in the microwell plate can be conveniently and quickly realized, experiment consumables and biological samples can be saved, the contamination risk can be reduced, and the experiment efficiency can be remarkably improved. Therefore, the working intensity of detection personnel is reduced, and the method has wide application value. Meanwhile, the complexity of detecting the growth state of the microorganisms in the pore plate is reduced, and popularization and application are facilitated.

The invention discloses a phycomycete symbiotic system and application of the phycomycete symbiotic system in treatment of aquaculture tail water, and belongs to the technical field of tail water treatment. The phycomycete symbiotic system comprises chlorella, scenedesmus and activated sludge, and the initial inoculation dry weight ratio of the chlorella to the scenedesmus to the activated sludge is 1: 1: 6. And exogenously adding octanoyl-L-homoserine lactone or tetradecanoyl-L-homoserine lactone into the phycomycetes symbiotic system, so as to construct the phycomycetes symbiotic system with enhanced functions. On the basis of a mixed algae-bacteria symbiotic system with an optimized ratio, directional enhancement of functions is realized by virtue of quorum sensing signal molecules, the stability and resistance gene interception capability of the quorum sensing signal molecules under the stress of antibiotics are verified, and finally efficient purification of the aquaculture tail water is realized. According to the invention, the blank of the prior art in the aspects of advanced regulation and control of a phycomycete synergistic mechanism and synergistic treatment of emerging pollutants is filled up.

The invention relates to the technical field of wastewater treatment or water quality purification, in particular to a method for synchronously culturing ammonia oxidizing bacteria and nitrite oxidizing bacteria, which comprises the following steps: selecting an inorganic material which is large in specific surface area and can quickly neutralize and release alkalinity as a nitrifying bacteria attachment carrier and an alkalinity supplement / pH regulator; the carrier is dissolved through nitration acid production, carbon dioxide and alkalinity are released, a carbon source and alkalinity required by growth are provided for nitrifying bacteria, and propagation of the nitrifying bacteria is promoted. Ammonia oxidizing bacteria and nitrite oxidizing bacteria are cultured synchronously by controlling the growth speed of the two bacteria in the culture process. By controlling the ammonia nitrogen concentration and nitrite concentration of the effluent at the end of culture to be lower than 50mg / L, sufficient time is provided for nitrite oxidizing bacteria to decompose an intermediate product nitrite, so that the purpose of simultaneously culturing AOB and NOB is achieved.

The invention discloses a microbial fertilizer with growth-promoting and yield-increasing effects and a preparation method thereof. The microbial fertilizer is prepared by mixing a hydrogel fertilizer and a microbial agent, the hydrogel fertilizer is formed by taking chitosan as a base material and carrying out cross-linking reaction with 2, 2-dimethyl-5-hexenoic acid, N-(N-butoxymethyl) acrylamide and urea, and the microbial agent is prepared by loading bacteria N9 separated from farmland soil on biochar. The microbial fertilizer has good water retention performance and nutrient slow release effect, the contained microorganisms can effectively promote growth, tests show that the soil water retention rate can be remarkably increased, growth of pakchoi and other crops is promoted, the biological safety is high, and the microbial fertilizer is suitable for achieving crop growth promotion and yield increase in agricultural production.

The invention provides a directional enrichment method of soil active organic carbon, a biochar-microorganism composite preparation and a preparation method, and relates to the technical field of soil improvement and agricultural sustainable development. The method comprises the following steps: (1) screening biochar; (2) screening and culturing a functional microbiome which at least comprises carbon-decomposing bacteria, nitrogen-fixing bacteria and phosphate-solubilizing bacteria, and compounding the carbon-decomposing bacteria, the nitrogen-fixing bacteria and the phosphate-solubilizing bacteria according to a CFU ratio of (1-5): (1-3): (1-2); (3) compounding the biochar in the step (1) and the functional microbiome in the step (2) by adopting an adsorption-embedding combined process to form a biochar-microbiome synergistic regulation system; and (4) applying the charcoal-microbiome coordinated regulation system obtained in the step (3) to target soil, and culturing. According to the invention, not only is input of soil organic carbon increased, but also accurate and efficient enrichment of the soil active organic carbon library is realized.

The invention belongs to the technical field of enzyme catalysis, and particularly relates to a dehydrogenase mutant, a gene, an engineering bacterium and application, the dehydrogenase mutant is obtained through mutation of original dehydrogenase, the sequence of the original dehydrogenase is SEQ ID NO.1, and the mutation mode is that the 344 site is mutated from A to W, K, F, S or N, or the 130 site is mutated from H to T, W or F; the dehydrogenase mutation diagram provided by the invention has higher conversion rate, stronger specificity and faster reaction speed.

The invention relates to the technical field of biology, in particular to application of an OgRKa gene and an upstream promoter thereof to improvement of rice bacterial blight and rice blast resistance, nucleotide sequences of the OgRKa gene and the upstream promoter are as shown in SEQ ID No.1, a CDS sequence is as shown in SEQ ID No.4, and an amino acid sequence of an encoding protein OgRKa is as shown in SEQ ID No.5. The homologous gene OgRKa of the rice MRKa gene (a rice plant over-expressing the MRKa gene has partial resistance to the rice bacterial blight, and a rice plant expressing the MRKa gene by using an MRKa self promoter has no resistance to the rice bacterial blight) is homologous cloned from oryza meyeriana through PCR (Polymerase Chain Reaction) amplification. Compared with the prior art, the resistance of rice plants with the OgRKa gene overexpressed by the 35S promoter or the OgRKa gene expressed by the promoter per se to a plurality of xanthomonas oryzae and magnaporthe oryzae is remarkably enhanced, the approximate immune level is achieved, the broad-spectrum high-resistance xanthomonas oryzae and magnaporthe oryzae are shown, and good application prospects are achieved in the aspect of breeding of xanthomonas oryzae and magnaporthe oryzae resistant varieties.

The application relates to a coking wastewater advanced treatment device, which comprises an aerobic tank, a horizontal shaft is arranged in the aerobic tank along the water flow direction, a plurality of coaxial rotating discs are arranged on the shaft at intervals, the rotating disc is hollow, the surface of the rotating disc is hollowed out, and the rotating disc is filled with bacteria culture fillers; a screw rod is arranged at the bottom of the inner side of the aerobic tank, the screw rod is parallel to the shaft, the same side ends of the shaft and the screw rod pass out of the aerobic tank, and the passing-out end of the shaft is in transmission connection with the passing-out end of the screw rod; a sludge discharge pipe is arranged at the bottom of the side wall of the aerobic tank, a vertical scraper is arranged at the bottom of the inner side of the aerobic tank, screw holes are arranged on the scraper, and the screw rod is matched with the screw holes. The bacteria film and the attachments on the surface of the fillers can be stripped through mutual collision and friction between the fillers, so that water flow exchange in and out of the fillers is smoother.

The invention discloses an oxidation ditch sludge state monitoring method and system, and relates to the field of computers, and the method comprises the steps: obtaining image data; generating unique metadata for each piece of image data; performing data annotation on the image data; recognizing the image data according to the image recognition model to obtain a recognition result; the metadata and the recognition result are fused, and cue words are constructed; inputting the cue word into the large model to obtain an analysis result; and generating a diagnosis report according to the analysis result. Through high-precision image monitoring and the deep reasoning analysis capability of a large model, the sludge state change is mastered in real time, so that the real-time, accurate and intelligent monitoring and diagnosis of key indexes such as sludge structure stability and filamentous bacteria abundance are realized.

The invention relates to the technical field of animal cub culture, in particular to an animal cub culture device for animal husbandry veterinarians, which comprises a culture box, a transparent door plate slidably connected to the culture box, a hollow plate inserted into the culture box, an excrement groove hung at the rear end of the culture box, a diversion groove formed in the lower end in the culture box, and a discharge groove formed in the rear wall of the culture box. The driving assembly is located in the incubator and comprises two movable blocks, the upper ends of the movable blocks are fixedly connected with push blocks, a guide rail is fixedly arranged on the top face of the upper end of the incubator, the upper ends of the two push blocks are slidably connected with the guide rail, and the outer walls of the two sides of each movable block are communicated with a plurality of nozzles. The flushed and disinfected area is dried through hot air, the cleaning effect of excrement of the cubs is improved, excrement residues are reduced, bacterium breeding is reduced, a healthy environment is provided for cub culture, and the morbidity of diarrhea, skin diseases and the like of the cubs is reduced.

The invention relates to the technical field of medicines, and discloses an application of a traditional Chinese medicine composition in a humanized flora transplantation model.The application comprises the following steps that an experimental body is pretreated through sterile feeding or broad-spectrum antibiotics, then coprophilous fungus suspension of a functional dyspepsia patient is transplanted, and the humanized flora transplantation model is constructed; acquiring physiological data of the humanized flora transplantation model, and performing intervention on the traditional Chinese medicine composition after the humanized flora transplantation model is completed; acquiring physiological data of the traditional Chinese medicine composition intervened humanized flora transplantation model, and analyzing an evaluation result of the traditional Chinese medicine composition intervened humanized flora transplantation model by combining the evaluation parameters of the humanized flora transplantation model. The bottleneck that the traditional animal model is greatly different from the human microflora and the curative effect and the mechanism are difficult to transform is overcome, so that the research result is closer to the clinical practice, and the molecular mechanism of the traditional Chinese medicine composition is reliably revealed.