6417 results about "Bacteria" patented technology

The present invention relates to the technical field of pesticides, and discloses a biological agent-based rice sheath blight disease efficient control agent, which comprises an active flora, a carrier, an auxiliary agent and a disease resistance enhancing factor, the active flora is used for combining humidity, pH and temperature response type materials so as to make the bacterial agent dynamically released, and the disease resistance enhancing factor is used for controlling the disease resistance of the rice sheath blight disease. The carrier is used for enabling the active ingredients to be slowly released in a field environment and keep stability, the auxiliary additive is used for improving the activity, adhesiveness and soil adaptability of the fungicide, and the disease-resistant enhancing factor is used for expanding and improving the adaptability of the control agent. The ratio of the active flora to the carrier to the auxiliary additive to the disease-resistant enhancing factor is 10: 50: 13: 27. Through the synergistic effect of various functional strains and the combination of the dynamic release carrier, the occurrence and propagation of the rice sheath blight disease are effectively inhibited, the prevention and treatment rate can reach 75-85%, and the prevention and treatment effect is obviously higher than that of a traditional chemical pesticide or a single fungicide.

The invention provides a genetically engineered bacterium, a preparation method thereof and application of the genetically engineered bacterium in synthesis of sclareol. The genetically engineered bacterium comprises a CcLPPS gene derived from Cistanus crenatus, and a CcLPPS gene derived from Cistanus crenatus, the genetically engineered bacterium is selected from yarrowia lipolytica. According to the invention, the stably expressed sclareol efficient de novo synthesis strain is constructed, and no organic reagent or any path intermediate substance is added in the whole fermentation process, so that the production cost is greatly reduced, and the subsequent separation and purification steps are simplified. The engineering yarrowia lipolytica strain provided by the invention is high in production intensity and simple in fermentation process, has a relatively good application prospect, and lays a foundation for producing sclareol by a microbial fermentation method.

The invention discloses a coal-based solid waste ecological restoration material and a preparation method thereof. The preparation method comprises the following steps: mixing coal gangue powder with a cementing material, a mineral activator and a physical pore-forming foam body, and curing to prepare a porous sandy soil matrix; and uniformly mixing the prepared porous sandy soil matrix with coal slime and a complex microbial inoculant, and performing fermentation and precipitation to obtain the coal-based solid waste ecological restoration material. Due to the addition of the cementing material, the strength of a sandy soil matrix framework is enhanced, collapse of pore channels in the crushing process is reduced, and the water retention and air permeability of the material are improved. Compared with traditional aluminum powder chemical foaming and strong acid foaming, the physical foaming technology adopted by the invention improves the operation safety while reducing the cost, and facilitates standardized production. Nutrient elements in the ecological restoration material can be fully released through the activation effect of the bacterium-fungus complex microbial inoculant. The ecological restoration material can be applied to environmental restoration fields such as mine soil restoration and land reclamation.

The invention relates to the field of agricultural soil improvement and ecological fertilization, and discloses an ecological fertilization method for improving the organic matter content of soil in a dry-hot valley region, and the method comprises the following steps: soil matrix improvement: improving the porosity and water binding capacity of the soil by adding charcoal and humic acid particles; the functional microbial preparation is put, and the activity of soil microorganisms is activated through the combined effect of cellulose decomposition bacteria, stabilization promoting flora and water retention flora; green manure crop planting and covering management: planting sweet clover and other leguminous crops to provide a carbon source and a nitrogen source for soil, and meanwhile, utilizing straw covering to inhibit water evaporation; turning over and stabilizing the green manure crops, namely turning over and stabilizing the organic matters in a blooming period of the green manure crops and combining with a stabilization promoting bacterial liquid and an adsorptive carrier; dynamic balance is maintained, and dynamic balance of decomposition, conversion and accumulation of organic matter is achieved step by step through multi-cycle planting and turning and pressing. The organic matter content and fertility of the soil can be improved, and the water-retaining property and microbial ecosystem of the soil are improved.

The invention provides a degradable environment-friendly packaging material and a preparation process thereof, and belongs to the technical field of packaging materials. The preparation method comprises the following steps: preparing the modified nano montmorillonite; preparing a composite antibacterial additive; preparing a barrier additive; and preparing the degradable environment-friendly packaging material. The preparation method comprises the following steps: by taking methacrylic acid (2-dimethylaminoethyl) ester and acrylic acid as raw materials, modifying nano-montmorillonite under the initiation of benzoyl peroxide, performing quaternary ammonium salinization, and performing in-situ synthesis of nano-zinc oxide on the surface of the quaternary ammonium salinized montmorillonite through a hydrothermal reaction to prepare the composite antibacterial additive. When the composite antibacterial additive is added into polylactic acid to prepare a packaging material, the growth of various microorganisms such as bacteria and fungi can be effectively inhibited, the packaging material is endowed with strong antibacterial protection capability, and the composite antibacterial additive has a more lasting antibacterial effect so as to prolong the antibacterial validity period of the packaging material.

Fracturing or fracking fluids for fracturing a subterranean reservoir for hydrocarbon recovery and related methods are described. The fluid comprises lignin, in particular technical lignin, and at least one strain of bacteria capable of biosurfactant production and / or a biosurfactant produced by at least one such isolated strain of bacteria. Also described is a fracturing method for recovering hydrocarbons from a subterranean formation.

The invention relates to an infectious bacterium detection method and system based on double effects of a reflection spectrum and autofluorescence, and belongs to the field of biomedical detection. The method comprises the following steps: acquiring hyperspectral images of different samples under different light sources; performing data preprocessing on the collected sample hyperspectral image, realizing pixel-level alignment of the dual-effect image based on feature matching and image registration, and constructing a data set; the method comprises the following steps: constructing a wound infection bacterium detection model based on double effects of a reflection spectrum and autofluorescence, and comprising a double-branch feature extraction module which comprises a reflection branch and a fluorescence branch which are respectively used for extracting reflection hyperspectral image features and fluorescence hyperspectral image features; the redundant feature screening module screens the features extracted by the reflection branch and the fluorescence branch through a channel attention mechanism; and the feature fusion module adopts a cross-branch cross attention mechanism to fuse the features of the double branches. According to the method, learning is carried out from two physicochemical characteristics, and the effectiveness and adaptability of the technology are improved.

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention provides an intracellular mycobacterium tuberculosis in-situ nucleic acid detection method based on CRISPR / Cas and application, and the method comprises the following steps: S1, designing crRNA and a primer pair of a mycobacterium tuberculosis target gene sequence, and synthesizing a single-stranded DNA fluorescent probe; s2, preparing a standard solution containing a target gene sequence, and incubating with a CRISPR / Cas12a system; s3, extracting nucleic acids of different types of bacteria, uniformly mixing the nucleic acids of different types of bacteria with the CRISPR / Cas12a system, and incubating; s4, incubating an attenuated strain of mycobacterium tuberculosis and the DNA fluorescent probe, infecting macrophages, and incubating to obtain infected cells; s5, electrically transferring a CRISPR / Cas12a system into the infected cells in S4 by utilizing an electroporation technology, incubating, and detecting the intensity of a fluorescence signal; and S6, performing quantitative and positioning analysis on the detected fluorescence signal to complete detection, and directly introducing a CRISPR / Cas12a system into the macrophage by adopting an electroporation technology to realize intracellular in-situ detection, thereby avoiding the operation complexity of a nucleic acid extraction link and the loss of detection sensitivity in the prior art.

To meet the need of a novel therapy for preventing or treating pathological dysbiosis of the gut microbiota, in particular for treating or preventing neurodegenerative diseases or bowel diseases. The present invention relates to the use of a composition comprising a plurality of specific molecules for the treatment of a pathological dysbiosis of the gut microbiota. The pathological dysbiosis of the gut microbiota may be characterized by an excess amount of sulfide-producing bacteria and insufficient butyrate-producing bacteria.

The invention discloses a high-activity short-cut nitrification quick-start long-term stable operation process, which relates to the technical field of biological sewage treatment, and comprises the following steps: inoculating sludge, carrying out anaerobic fermentation pretreatment, and removing expanded sludge and sludge foam in combination with quick sludge discharge and manual removal; controlling the number of days of sludge age, reproliferating the sludge, enriching ammonia oxidizing bacteria, and inhibiting and elutriating nitrite oxidizing bacteria; long-term operation is carried out under the condition of no sludge discharge, the abundance and activity of ammonia oxidizing bacteria are maintained, and meanwhile, the abundance of nitrite oxidizing bacteria is controlled; the proliferation of nitrite oxidizing bacteria is controlled by using the predation effect of protozoa and metazoan, and the accumulation rate of nitrite in the reactor is controlled; monitoring the dissolved oxygen, the pH value and the concentrations of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the reactor in real time, and regulating and controlling the influent ammonia nitrogen load and the free ammonia concentration; dissolved oxygen distribution is adjusted in an intermittent aeration or microporous aeration mode, and the operation conditions of the short-cut nitrification reactor are optimized. The method is simple to operate, economical and effective.

The invention relates to the technical field of inorganic non-metallic materials, in particular to a quaternized cholic acid polyurethane modified polyvinyl chloride material, a preparation method and application. The quaternized cholic acid polyurethane polymer is based on a polyurethane elastomer developed by using cholic acid molecules with a rigid skeleton as a hard segment unit and flexible oligomeric ethylene glycol as a soft segment unit, hydroxyl on cholic acid polyurethane is quaternized to prepare cation modified cholic acid polyurethane, and the cation modified cholic acid polyurethane simulates amphiphilic characteristics of natural peptides. The quaternized cholic acid polyurethane is introduced into a polyvinyl chloride (PVC) matrix as an antibacterial agent, so that the growth of microorganisms such as bacteria and molds can be effectively inhibited, the antibacterial property is achieved, bacteria breeding on the inner surface of a pipeline is avoided, and the composite material is endowed with better biocompatibility and antibacterial property; meanwhile, the quaternized cholic acid polyurethane can effectively improve the strength and toughness of the PVC material; wide application prospects are realized.

The invention provides a method for detecting influence of microplastics and antibiotics on a sediment microbial community structure and an ecological function. The method comprises the following steps: acquiring a sediment sample at a sampling point; adding the micro-plastic particles and the antibiotic solution into the sediment sample, and incubating; dNA of the microorganisms is extracted, and 16S rRNA sequencing is carried out; and on the basis of a sequencing result, microbial community diversity and characteristic bacterial class group identification and ecological function evaluation are carried out. According to the invention, the comprehensive effect of micro-plastic and antibiotic combined pollution on the structure and ecological function of the microbial community in the sediment is comprehensively analyzed for the first time, and the detection speed and accuracy of the microbial community in the sediment are improved by applying a 16S rRNA detection technology; and a solid support is provided for formulating an environment protection strategy, optimizing pollution control and implementing an ecological restoration project.

The utility model discloses a novel hydrolytic acidification modular biological filler frame, which comprises a treatment tank body and a plurality of hydrolytic acidification modules, the hydrolytic acidification modules are fixedly connected inside the treatment tank body through fixing components, and each hydrolytic acidification module comprises a carbon fiber filler frame, an electromagnet block, an arc-shaped frame and a net-shaped cover plate, a plurality of carbon fiber filaments distributed in a radial shape are arranged in the carbon fiber filler frame, are filamentous fillers and are used for loading hydrolytic acidification bacteria, and the plurality of carbon fiber filaments are fixed by stainless steel wires and are connected in series. According to the utility model, the carbon fiber yarns are radially distributed in the carbon fiber filler frame, so that the attachment area of microorganisms is greatly increased, an abundant growth space is provided for the microorganisms, rapid propagation and efficient metabolism of the microorganisms are promoted, and the degradation efficiency of pollutants is also remarkably improved.

The invention provides a low C / N sewage nitrogen and phosphorus removal method based on MABR-DPR-anaerobic ammonia oxidation, which couples anaerobic, membrane aeration anoxic and anaerobic ammonia oxidation processes, forms an anoxic environment in a water phase of a membrane aeration reactor, and enriches denitrifying phosphorus-accumulating bacteria. And the anaerobic phosphorus release and anoxic denitrification nitrogen and phosphorus removal processes of the denitrifying phosphorus-accumulating bacteria are realized through the backflow of the sludge between the anaerobic tank and the membrane aeration reactor. Nitrite accumulated in effluent of the membrane aeration reactor supplements an electron acceptor for a subsequent anaerobic ammonia oxidation deep denitrification process, and meanwhile, a carbon source is obviously consumed in an anoxic denitrification nitrogen and phosphorus removal process of the membrane aeration reactor, so that inhibition of influent organic matters on an anaerobic ammonia oxidation process is effectively weakened. The process solves the problem that the carbon source is seriously insufficient in the nitrogen and phosphorus removal process of the low C / N sewage, and the stable and efficient nitrogen and phosphorus removal effect can still be maintained under the condition that an external carbon source is not needed.

The utility model discloses an automatic assembling machine for a bacterium-blocking membrane assembly of a dosing pump head, which comprises a machine base, a cam driving device in the machine base and a rotary material conveying mechanism, and a product jig is arranged on the rotary material conveying mechanism. An inner plug feeding mechanism, a filter membrane feeding mechanism, a shell feeding mechanism, a pressing mechanism, a product detection mechanism and a discharging mechanism are sequentially arranged on the machine base in the movement direction of the rotary material conveying mechanism. The inner plug feeding mechanism is used for placing an inner plug on the product jig, the filter membrane feeding mechanism is used for cutting a filter membrane and can place the filter membrane on the product jig, the shell feeding mechanism is used for placing a shell on the product jig, and the pressing mechanism is used for pressing the shell to be connected to the periphery of the inner plug in a sleeving mode. The product detection mechanism is used for detecting whether products on the product jig are qualified or not, and the discharging mechanism is used for moving out the products on the product jig. According to the bacteria blocking module assembling machine, the bacteria blocking module assembly can be automatically assembled, manual participation is not needed, the labor cost is reduced, and the assembling efficiency and the yield can be improved.

The invention relates to the technical field of flower planting substrates, and discloses a general type flower fertilization-free planting substrate based on nitrogen-fixing, phosphorus-dissolving and potassium-dissolving bacteria, which comprises turfy soil, perlite, ground phosphate rock, straw, carbonized rice husk, humic acid, seaweed oligosaccharide, nitrogen-fixing bacteria, phosphorus-dissolving bacteria and potassium-dissolving bacteria. Turfy soil and perlite are used as a substrate and compounded with ground phosphate rock, straw, carbonized rice husks, humic acid, seaweed oligosaccharide, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria, the nitrogen-fixing bacteria provide nitrogen for flowers, and the phosphate solubilizing bacteria and the potassium solubilizing bacteria decompose phosphorus and potassium in the ground phosphate rock, the straw and the carbonized rice husks to provide nutrition for the flowers; the humic acid and the seaweed oligosaccharide have the effects of promoting rooting and growth of the flowers and breeding of beneficial microorganisms; all the internal components have a synergistic effect, the fertilization-free period is extremely long, essential elements for plants are still efficiently provided under the condition of no fertilization, the growth of the plants and the development of flowers are remarkably promoted, the use of toxic substances is reduced, meanwhile, the low incidence rate of diseases and insect pests is ensured, and better flower growth and ornamental effects are achieved on the whole.

The present disclosure provides compositions, kits, and systems comprising engineered bacteria and agricultural biologics, and methods of using the same to treat plants and improve one or more plant beneficial traits, such as plant yield, nutrient uptake, and resistance to abiotic or biological stress. Using the compositions, kits, and systems disclosed herein can further 5 reduce fertilizer usage. The present disclosure also proposes methods of treating plants using the engineered bacteria with agricultural biologics.

The invention discloses a rapid concrete repairing material based on microbial mineralization as well as a preparation method and application of the rapid concrete repairing material. According to the method, hydrophobic modified porous particles are used as a carrier, microbial bacteria, nutrient substances and a calcium source with mineralization behaviors are soaked into pores of the porous particles through vacuum impregnation, the pores of the particles are properly sealed by adopting waterborne epoxy, and then the particles are packaged by using metakaolin and sodium silicate, so that the porous particles are obtained. And finally, compounding with fiber composite mortar to prepare the concrete rapid repairing material. The rapid concrete repairing material has the advantages of strong interface adhesion, environment friendliness, light weight, excellent surface compactness improvement effect, crack self-repairing and the like, and can be used for coating and repairing the defect surface of concrete.

The invention relates to a river ecosystem planktonic bacteria environment threshold identification method, and belongs to the field of environmental ecology and computational biology. The method comprises the following steps: collecting a plurality of environmental factors, fusing and constructing a space-time aligned watershed ecosystem data set, and preprocessing the data set; constructing a prediction model and performing training; environmental factors are collected from a target drainage basin and input into the trained prediction model, and a nonlinear mapping relation between the environmental factors and the microbial community, namely an SHAP value, is obtained through learning; constructing a GAM response curve based on the SHAP values and the environmental factors, fitting a nonlinear response trend of the GAM response curve through a spline function, and identifying an environmental threshold corresponding to each environmental factor; through a Bootstrap resampling method, multiple times of environment threshold recognition are carried out on each environment factor, and a confidence interval is estimated based on environment thresholds obtained through multiple times of recognition, so that the stability and reliability of environment threshold recognition are improved.

The invention relates to the technical field of component monitoring, and discloses a compost fermentation process material component intelligent monitoring method comprising the following steps: collecting compost process environment data and compost process material component data in a compost process, and carrying out thermophilic bacteria metabolic activity on-line prediction to obtain a flora metabolic activity index; receiving the composting process environment data, the composting process material component data and the flora metabolism activity index by using the lightweight material component inversion model, and generating an optimal material component ratio; and comprehensively adjusting the material components in the composting fermentation process by using the optimal material component ratio. According to the method, environmental data and material component data in the composting process are collected in real time, the metabolic activity index of thermophilic bacteria is predicted on line, the optimal material component proportion is generated on the basis of a lightweight material component inversion model in combination with the environmental data, the material component data and the metabolic activity index, and the material components in the composting fermentation process are comprehensively adjusted, so that the composting fermentation efficiency is improved. Therefore, the composting effect is optimized.

The present invention discloses an environmental monitoring system and a monitoring method, which relates to the field of environmental monitoring technology. The system accurately collects the abundance vector MAV of the microbial community within a fixed period, performs concentration and separation treatment, then adds a substrate reaction and monitors the enzyme catalytic reaction in the water sample in real time, generates an enzyme activity feature set EAF, combines the aerobic bacteria change rate Raer, the anaerobic bacteria change rate Ranaer and the correction factor F, forms a multidimensional data set Eac, and establishes a comprehensive water quality assessment algorithm based on this, thereby obtaining a comprehensive water quality detection and assessment index WQI, and realizing a quantitative assessment of the health status of water quality. By comparing with the water quality assessment standard threshold Sthe, an early warning notification is automatically triggered, water quality abnormalities are discovered and responded to in a timely manner, solving the problem that the traditional method cannot capture water quality changes in a timely manner, especially in terms of rapid response to environmental pollution and ecological changes. It has significant advantages.

The invention discloses an aspergillus niger recombinant bacterium AnCT-XynB-K-agdA and application thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, Aspergillus niger AnCat is taken as an expression host, firstly, an acid protease regulatory factor prtT is knocked out to obtain a defective strain, and then different chaperonins are subjected to fusion expression by optimizing integration sites of a laccase lcc9 expression cassette, so that the high-expression laccase chaperonin is obtained. According to the method, an expression vector pC3-5 'agdA-PcitA-XynB-L-lcc9-hph-3' agdA is constructed, a recombinant bacterium AnCT-XynB-K-agdA is further obtained, the enzyme activity of the extracellular laccase subjected to shake flask fermentation reaches 1821.2 U / L, and finally, the recombinant efficient expression of the laccase Lcc9 in aspergillus niger is realized.

The invention relates to the technical field of ecological restoration, in particular to a preparation method of novel vegetation concrete greening additive AB bacteria, which comprises the following steps: step (1) screening and activating double bacteria; (2) preparing an inner core structure; (3) loading double bacteria; (4) coating a shell layer; (5) loading a functional matrix; and (6) preparing the vegetation concrete greening additive AB bacteria. According to the method, a multi-layer and function-synergistic microorganism-seed-carrier composite system is constructed and used for surface concrete attachment of the high steep slope, and a plant-microorganism symbiotic system with physical isolation, chemical buffering and nutrition self-sustaining capacities is actively constructed for plant seeds and functional microorganisms in a strongly alkaline macroscopic environment; and finally, the long-acting ecological stability of the high and steep slope is realized.

The invention discloses a dual-mechanism antibacterial photosensitizer as well as a preparation method and application thereof, and relates to the technical field of biomedical photoelectric functional materials. The invention relates to a triphenylamine derivative, which can realize fluorescence imaging of bacterial or fungal infection by utilizing fluorescence characteristics, can generate active oxygen under illumination to play a photodynamic therapy role, is suitable for preparing photodynamic therapy preparations and developing antibacterial materials, and can also be applied to the fields of environment and public health and tumor treatment. The problems that an existing photosensitizer is insufficient in broad spectrum, different in fungus drug resistance mechanism, limited in single treatment effect and the like can be effectively solved, and the photosensitizer has wide application prospects.

The present application relates to the field of municipal wastewater treatment, and particularly relates to a highway service area wastewater treatment device and method based on AOOA-MBBR process. The device comprises a first anoxic tank, a first aerobic tank, a second aerobic tank and a second anoxic tank connected in sequence, and is filled with MBBR filler respectively, and the volume of the filled MBBR filler is taken as the effective volume; the first aerobic tank and the second aerobic tank are respectively provided with an aeration device; the first anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the first aerobic tank is used for inoculating hydrolytic bacteria, nitrifying bacteria and denitrifying bacteria; the second aerobic tank is used for inoculating nitrifying bacteria, and the second anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the second aerobic tank is connected with the first anoxic tank through a reflux pipe, and the reflux pipe is provided with a reflux power assembly. The present application solves the problems of existing highway service wastewater treatment with extremely low carbon-nitrogen ratio, such as the need for additional carbon source / electron donor, high operation cost, poor denitrification effect and high sludge yield.

The invention relates to the technical field of microbial electrochemistry, in particular to a microbial fuel cell self-driven microbial electrolysis cell system and application thereof. The microbial fuel cell self-driven microbial electrolysis cell system comprises a microbial electrolysis cell (MEC) and a microbial fuel cell (MFC) for supplying power to the MEC; an anode electrode of the MFC is a mixed strain immobilized modified carbon felt electrode, and a cathode electrode of the MFC is a modified carbon felt electrode; an anode electrode and a cathode electrode of the MEC are independently selected from one of a modified carbon felt electrode or a mixed strain immobilized modified carbon felt electrode; the mixed strain is a strain obtained by mixing shewanella and sludge bacteria; the anode electrode of the MEC is connected with the cathode electrode of the MFC, and the anode electrode of the MFC is connected with the cathode electrode of the MEC; the bipolar chambers of the MFC are both in an anaerobic state, and the bipolar chambers of the MEC are both in an anoxic state. The advantages of an MFC-MEC system are fully utilized, external power supply is not needed, the MFC supplies energy to the MEC to promote reverse flow of electrons, and electric energy is utilized in situ.

The invention provides high-durability concrete for inducing adhesion of marine fixing organisms and a preparation method, and belongs to the crossing field of marine fixing organisms and marine concretes.The concrete is prepared by adopting a porous carrier loaded with induction bacteria, and the impregnation process of the porous carrier loaded with the induction bacteria is optimized; according to the present invention, the barnacle can be induced to adsorb to the concrete surface, the chloride ion resistance of the concrete can be improved, and the concrete has characteristics of simple component, low preparation cost, green environmental protection, and wide application prospect, and can provide more effective protection for the loaded bacteria, and can induce the barnacle to adsorb to the concrete surface. The goal of high durability of the concrete for underwater ocean engineering can be achieved.

The invention relates to a cationic biomass-based nano enzyme as well as a preparation method and application thereof. The cationic biomass-based nano-enzyme comprises a biomass-based nano-enzyme and an electropositive substance for modifying the biomass-based nano-enzyme, and the biomass-based nano-enzyme comprises a tea seed cake extract, fulvic acid and a metal salt. The cationic biomass-based nano-enzyme can enhance the stress resistance and activity of microbial flora, and the preparation method is green, environment-friendly, simple and convenient.

The invention discloses an identification and analysis method for unknown pathogenic microorganisms, which comprises the following steps: filtering out genome sequences with low integrity, pollution and tag errors, and establishing a high-quality virus identification database; constructing a virus host prediction model through a machine learning algorithm; unknown pathogenic microorganisms are identified and analyzed, potential hosts or pathogenicity of the unknown microorganisms are identified, and whether the unknown microorganisms are unknown pathogenic viruses or bacteria or not is further judged. On the basis of metagenome data analysis, potential unknown pathogenic microorganisms in samples of human bodies, environments and the like can be identified more accurately.