65 results about "EPSP synthase" patented technology

Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.

Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 4, 6, 8, 10, 12, or 14 or the nucleotide sequence set forth in SEQ ID NOS:1, 3, 5, 7, 9, 11, 13, 28, 29, 30, 31, 32, 33, or 34.

Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include novel EPSP synthase enzymes and nucleic acid molecules encoding such enzymes, vectors comprising those nucleic acid molecules, and host cells comprising the vectors.

Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 6, 7, 9, 10, 12, 13, 15, 17, 18, 20, 21, or 23, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, 5, 8, 11, 14, 16, 19, or 22, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30932, B-30933, B-30934, B-30945, B-30946, B-30947, or B-30948, as well as variants and fragments thereof.

Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1 or 14, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30931, as well as variants and fragments thereof.

Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.

Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include novel EPSP synthase enzymes and nucleic acid molecules encoding such enzymes, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.

Disclosed is a herbicidal mixture comprising (a) at least one herbicide compound selected from the pyrimidines of Formula 1, including all geometric and stereoisomers, N-oxides, and salts thereof:whereinR1 is cyclopropyl, 4-Br-phenyl or 4-Cl-phenyl;X is Cl or Br;R2 is H, C1-C14 alkyl, C2-C14 alkoxyalkyl, C3-C14 alkoxyalkoxyalkyl, C2-C14 hydroxyalkyl or benzyl; and(b) at least one additional herbicide or herbicide safener compound selected from the group consisting of (b1) ACCase inhibitors, (b2) AHAS inhibitors, (b3) photosystem II inhibitors, (b4) photosystem I electron diverters, (b5) PPO inhibitors, (b6) EPSP synthase inhibitors, (b7) GS inhibitors, (b8) VLCFA inhibitors, (b9) auxin mimics, (b10) auxin transport inhibitors, (b11) other herbicides selected from the group consisting of flamprop-M-methyl, flamprop-M-isopropyl, difenzoquat, DSMA, MSMA, bromobutide, flurenol, cinmethylin, cumyluron, dazomet, dymron, methyldymron, etobenzanid, fosamine-ammonium, isoxaflutole, asulam, clomazone, mesotrione, metam, oxaziclomefone, oleic acid, pelargonic acid and pyributicarb, (b12) herbicide safeners selected from the group consisting of benoxacor, 1-bromo-4-[(chloromethyl)sulfonyl]benzene, cloquintocet-mexyl, cyometrinil, dichlormid, 2-(dichloromethyl)-2-methyl-1,3-dioxolane, fenchlorazole-ethyl, fenclorim, flurazole, fluxofenim, furilazole, isoxadifen-ethyl, mefenpyr-diethyl, methoxyphenone, naphthalic anhydride and oxabetrinil, and their salts. Also disclosed is a method for controlling the growth of undesired vegetation comprising contacting the vegetation or its environment with a herbicidally effective amount of a mixture of the invention (e.g., as a composition described herein).

An isolated polypeptide comprising an amino acid sequence that is at least 62% identical to SEQ ID NO:2. The polypeptide, when present in a cell, increases the cell's ability to tolerate glyphosate. Also disclosed are related nucleic acid, antibody, vector, transgenic plant, as well as uses thereof.

An isolated polypeptide comprising an amino acid sequence that is at least 62% identical to SEQ ID NO:2. The polypeptide, when present in a cell, increases the cell's ability to tolerate glyphosate. Also disclosed are related nucleic acid, antibody, vector, transgenic plant, as well as uses thereof.

The present invention discloses one kind of 5-enolpyruvoyl shikimic acid-3-phosphosynthase mutant and its coding gene, obtaining method and application in culturing glyphosate resisting plant. The 5-enolpyruvoyl shikimic acid-3-phosphosynthase has the residue sequence as shown in SEQ ID No. 2, and will take important role in the culture of glyphosate resisting plant.

The invention discloses a novel 5-Enolpyruvylshikimate-3- phosphate synthase ( abbreviated as ' EPSP Synthase'), which is to generate EPSP Synthase with polynucleotide coding the EPSP Synthase by a recombination technique. The invention also discloses applications of the polynucleotide coding the EPSP Synthase.

The invention provides a corn chloroplast transgene expression vector containing herbicide resistant gene and application thereof. The transgene expression vector comprises an expression box of 5-enol form acetone shellokimic acid-3-phosphate synthase (EPSP) gene, a homologous recombination fragment atpB in a corn chloroplast gene group and a homologous recombination fragment rbcL, wherein the expression box of the EPSP synthase gene is positioned between the homologous recombination fragment atpB and the homologous recombination fragment rbcL. The invention further provides a construction method of the chloroplast transgene expression vector. The invention also provides application of the chloroplast transgene expression vector in improving corn glyphosate resistance, which comprises leading the expression vector to convert callus or cells of corns, and screening to obtain resistant callus or cells; and inducing resistant callus or cells to differentiate, and regenerating to achieve corn resistance plants. The corn chloroplast transgene expression vector containing herbicide resistant gene can remarkably improve corn glyphosate resistance and has the advantages of being high in biological safety, capable of saving time, efficient, little in energy consumption, low in cost and the like.

The invention discloses a glyphosate resistance enhanced EPSP (5-enolpyruvyl shikimate-3-phosphate synthase) synthase multi-site mutant which is derived from Vitis vinifera and prepared by adopting a DNA shuffling technology, as well as a coding gene and application of the mutant. The mutant comprises 7 mutation sites, wherein the amino acid sequence of the mutant is as shown in the SEQ ID No.1, and the base sequence coded by the mutant is as shown in the SEQ ID No.2. Tests indicate that the EPSP synthase multi-site mutant derived from Vitis vinifera and the gene coded by the mutant have relatively high glyphosate resistance and relatively strong PEP affinity, and the characteristics of the mutant and the gene provide possibility to the gene for culturing anti-glyphosate transgenic crops.

The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be used for culturing genetically modified crops.

The invention relates to an EPSP synthase AroA-Ra multisite mutant of a rahnella aquatilis, particularly to the multisite mutant improved from the gene coded as 5- enol acetone shikimic acid-3-EPSP synthase of the rahnella aquatilis through DNA molecular rearrangement method and function complementation method, and the resistance of the improved multisite mutant to the glyphosate is greatly improved. The nucleotide sequence of the multisite mutant is as indicated by SEQ ID No1, the encoded amino acid sequence is as indicated by the SEQ ID No2, and the mutant has 6 different mutational sites under the same molecule. The multisite mutant not only retains the high affinity of the original EPSP synthase toward the PEP, but also reduces the affinity of the EPSP synthase toward the glyphosate herbicide, so that the glyphosate resistance of the mutant is greatly improved.

Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include a novel class of EPSPS enzymes, designated Class III, and polynucleotides encoding such enzymes, vectors comprising those polynucleotides, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the Class III domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.

The invention provides a novel 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase) and a coding gene thereof. The EPSP synthase is (1) a protein composed of amino acid sequence disclosed as SEQ ID NO.2, or (2) a (1)-derived protein with glyphosate tolerance and EPSP synthase activity obtained by substitution, deletion or addition of one or more amino acids on the basis of the amino acid sequence disclosed as SEQ ID NO.2. The invention also provides a preparation method of the EPSP synthase, and application of the EPSP synthase and coding gene thereof. After the EPSP synthase coding gene is transplanted and expressed into a plant, the obtained transgenic plant has higher tolerance to glyphosate.

The invention discloses an EPSP (5-enolpyruvyl shikimate-3-phosphate) synthase multisite mutant from Malus domestica, and a coding gene and application of the mutant. The mutant has 8 mutation sites, the amino acid sequence of the mutant is as SEQ ID No. 1, and the base sequence of the coding gene of the mutant is as SEQ ID No. 2. Tests show that the EPSP synthase multisite mutant from Malus domestica and the coding gene thereof have high glyphosate resistance, high PEP (phosphoenolpyruvic acid) affinity is kept, and accordingly possibility for using the coding gene for cultivation of transgenic crops is provided.

An EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) with high glyphosate resistance and a nucleotide sequence encoding the synthase are disclosed. The gene encoding the EPSP synthase has low homology with the reported EPSP synthase. A transgenic plant obtained by the expression of the gene in plant has an increased resistance to glyphosate after experimental confirmation.

The invention discloses an EPSP synthase gene from Klebsiella pneumoniae 342 and application of the EPSP synthase gene. The EPSP synthase gene comprises 1284 basic groups and 428 coding amino acid, the nucleotide sequence is shown as SEQ ID NO 1, and the coding amino acid sequence is shown as SEQ ID NO 2. The EPSP synthase gene from the Klebsiella pneumoniae 342 has high glyphosate tolerance and can be used for cultivating transgenic crops.

Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO: 1, 3, 4, 6, 1, 9, 10, 12, 13, 15, 17, 18, 20, 21, or 23, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, 5, 8, 11, 14, 16, 19, or 22, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30932, B-30933, B-30934, B-30945, B-30946, B-30947, or B-30948, as well as variants and fragments thereof.

The invention discloses a novel 5-enol pyruvyl shikimate3-phosphate (EPSP), the polynucleotide for encoding the EPSP and the method for producing the EPSP through recombination technology. The invention also discloses the use of the polynucleotide for encoding the EPSP.

The invention discloses a 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase gene from ochrobactrum anthropi and application thereof. The invention also provides genetic engineering intermediates (such as an expression cassette, a carrier and a cell) of the gene, a method for obtaining a plant with glyphosate resistance, and application of the method, and provides an identification method for judging whether the plant has the glyphosate resistance. The EPSP synthase gene plays an important role in research and development of the glyphosate-resistant plant.

The invention relates to polynucleotide of an insect resistant plant resistant to herbicide, an expression cassette including polynucleotide and application thereof. The polynucleotide of an insect resistant plant resistant to herbicide includes first polynucleotide, second polynucleotide and third polynucleotide. The first polynucleotide includes a coding area of EPSP synthase; the second polynucleotide includes a coding area of a vegetative period insecticide protein VIP3A; and the third polynucleotide includes a coding area of an insecticide protein Cry2A. The polynucleotide can code the EPSP synthase, the vegetative period insecticide protein VIP3A, and bacillus thuringiensis Delta-endotoxin, i.e., the insecticide protein Cry2A. The invention further relates to a DNA builder formed by a first expression cassette, a second expression cassette, a third expression cassette and a fourth expression cassette, and a method for transforming a plant by using the DNA builder. The transgenic plant has stronger resistance to lepidoptera insects than a first generation single-gene Bt plant through enhanced coordinate expression, and has a herbicide resistance character, thereby facilitating farmland weeding.

The invention relates to a glyphosate-resistant herbicide gene AroA-Ra from grape crown gall antagonistic bacteria rahnella aquatilis and application thereof, particularly to a gene coded as 5- enol acetone shikimic acid-3-EPSP synthase of the rahnella aquatilis having antagonistic function against the grape crown gall from the vineyard through function complementation method, and the application to the improvement of the resistance of the plant transformed from the gene to the glyphosate herbicide.

Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 4, 6, 8, 10, 12, or 14 or the nucleotide sequence set forth in SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13, 28, 29, 30, 31, 32, 33, or 34.