34results about How to "Small molecular weight" patented technology

This invention belongs to the field of photocrosslinking probe technology, specifically relating to a GUSB photocrosslinking probe, its preparation method, and its application. The GUSB photocrosslinking probe has the general chemical formula C0. 13 H 18 N2O6-R, wherein R is any one of the oxalic group elements or peptide bonds. The R group covalently connects the glucuronic acid recognition core and the photosensitive unit in the GUSB photocrosslinking probe, and the R group contains no more than 4 atoms, resulting in a small size and low steric hindrance. The GUSB photocrosslinking probe provided by this invention uses glucuronic acid as the recognition core, combined with a small-volume diazacyclopropane photosensitive unit and a terminal alkyne group, to achieve µM-level in-situ covalent capture of active GUSB in complex systems such as cells or tissues. The probe of this invention significantly improves positioning accuracy, sensitivity, data availability, quantification, and enrichment, while also possessing advantages such as modular synthesis, mild conditions, and strong system compatibility, effectively addressing the technical shortcomings of existing technologies that struggle to accurately obtain GUSB activity in situ under low-dose conditions.

The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.

This invention discloses a micropeptide, its encoding nucleic acid, and its application in regulating inflammatory activation of microglia. The amino acid sequence of the micropeptide is shown in SEQ ID NO:3. Simultaneously, this invention discloses the nucleic acid encoding the micropeptide, which contains either the nucleotide sequence shown in SEQ ID NO:1 or the nucleotide sequence shown in SEQ ID NO:2, wherein SEQ ID NO:2 is a small open reading frame located within the sequence of SEQ ID NO:1. This invention validated the translational expression of the micropeptide in microglia by constructing an EGFP reporter gene fusion vector and a SUMO tag fusion expression vector. Functional experiments showed that under oxygen-glucose deprivation / reperfusion injury conditions, the micropeptide significantly inhibited inflammatory activation of microglia. This invention provides a new potential target and candidate drug for the treatment of neuroinflammatory diseases such as stroke.

This invention belongs to the field of molecular biology and medical detection technology, and discloses a bladder cancer-specific nucleic acid aptamer, nucleic acid aptamer derivatives, and their applications. The nucleotide sequences of the nucleic acid aptamer are shown in SEQ ID NO: 1, 2, and 3. This invention screened three monoclonal nucleic acid aptamer DNA chains from bladder cancer tissue samples using the exponential enrichment systematic evolution (SELEX) technique and identified two target proteins that bind to these three nucleic acid aptamers. The nucleic acid aptamers specifically target the target proteins CAPRIN1 and FXR1 on the surface of bladder tumor cells. The nucleic acid aptamers obtained by this invention have advantages such as high affinity and specificity, no immunogenicity, simple chemical synthesis, stability, and ease of storage and labeling, providing a new molecular tool for the early diagnosis and targeted therapy of bladder cancer.

This invention discloses an environmentally friendly plasticizer using modified lignin as a carrier, a rubber composition, and a method for preparing the same, relating to the field of rubber processing technology. The environmentally friendly plasticizer using modified lignin as a carrier, by weight, comprises the following components: 50 parts modified lignin, 25 parts plasticizing active ingredient, 8 parts dispersant, 5 parts anti-aging agent, and 3 parts stabilizer. The modified lignin is a lignin oligomer containing ester, acetyl, and ether cross-linked structures, with a weight-average molecular weight of 800-1200 Da, a dispersion coefficient ≤1.5, and a carboxyl content ≤0.2 mmol / g. This invention successfully overcomes the technical defects of existing plasticizer carriers, such as high toxicity, difficulty in degradation, and poor compatibility with polymer matrices, providing an environmentally friendly plasticizer using modified lignin as a carrier. This technical solution achieves the dual goals of high-value utilization of lignin and greening of the plasticizer product.

This invention relates to the field of ginseng extract technology, specifically to a composition containing ginseng extract and its preparation method. The provided composition containing ginseng extract comprises the following substances in parts by weight: 5-30 parts of a ginseng peptide composition with a molecular weight less than 3000 Da; 1-15 parts of a saponin composition composed of rare ginsenosides Rg3, Rg5, and Rh2; 3-10 parts of a ginseng polysaccharide-selenium nanocrystal composition; 1-5 parts of *Lactobacillus plantarum* P-8 lyophilized powder; 0.5-3 parts of sodium tanshinone; 3-20 parts of a prebiotic composition; and 0.1-5 parts of a vitamin composition. A method for preparing the aforementioned ginseng extract composition is also provided. The prepared ginseng extract composition exhibits excellent properties and has good application prospects.

The application belongs to the technical field of biological medicine, and particularly relates to an anti-CD22 nanobody and a preparation method and application thereof. The anti-CD22 nanobody comprises a framework region and a complementarity determining region, wherein the complementarity determining region comprises CDR1 (SEQ ID NO: 1), CDR2 (SEQ ID NO: 2) and CDR3 (SEQ ID NO: 3). The preparation method comprises the following steps: immunizing a llama with a Human CD22 / His protein, extracting RNA from PBMC cells and reverse transcribing the RNA into cDNA, constructing a phage display library, obtaining a positive clone through CD22 antigen protein panning after sequencing analysis, and expressing the antibody through a mammalian cell expression system. The application adopts an optimized phage display technology, has a short screening cycle, and the obtained nanobody has a small molecular weight, a stable structure and high affinity to CD22. The antibody can be efficiently expressed through a mammalian cell, and retains natural modification activity.

The application relates to the technical field of wood decoration materials, and particularly discloses a wood surface composite structural color protective layer and a preparation method. The application first self-assembles and constructs a structural color layer on the wood surface; then applies PVB ethanol solution, and forms a PVB layer which internally strengthens the structural color layer in a dot-shaped bonding mode after drying; and finally coats UV paint and forms an outer protective layer through ultraviolet light curing. The PVB layer of the application effectively strengthens the cohesive force and the adhesion to the substrate without destroying the order of the structural color layer; the UV paint layer is firmly combined with the PVB layer through possible chemical bonding, and excellent wear resistance and scratch resistance are provided. The two are synergistic, solve the technical problem that the adhesion of the traditional wood surface structural color layer is poor and the structural color layer is easily damaged, and the prepared color protective layer is bright in color and has a wide application prospect.

The application discloses a transcritical CO2 air source heat pump system and a control method thereof. The transcritical CO2 air source heat pump system comprises a carbon dioxide compressor, a gas cooler, an electronic expansion valve, an outdoor fan, a cold storage device, a heat storage water tank, a high-temperature heat accumulator, a low-temperature heat accumulator and a floor buried pipe heat exchanger. The gas cooler comprises a first working medium pipeline and a first heat exchange pipeline for realizing heat exchange. The cold storage device comprises a second working medium pipeline and a second heat exchange pipeline for realizing heat exchange. The heat storage water tank comprises a hot end heat exchange pipeline and a cold end heat exchange pipeline for realizing heat exchange. The transcritical CO2 air source heat pump system can be controlled to be in various working conditions, such as single hot water production, refrigeration and hot water production, single heating, heating and hot water production and the like, by controlling the opening and closing of each component and valve, and is integrated and multipurpose to adapt to different requirements.

This invention relates to the field of biomedical technology, and more particularly to a PD-L1-targeting polypeptide, its conjugate, and its applications. The polypeptide possesses the amino acid sequence shown in SEQ ID NO.1 or SEQ ID NO.2. This invention de novo designs a high-affinity PD-L1-targeting specific polypeptide. This polypeptide achieves extremely high affinity with the extracellular domain of the PD-L1 protein through its unique spatial conformation, exhibiting excellent targeting and recognition capabilities. The polypeptide provided by this invention can be conjugated with various modified molecules, such as radionuclides, and the constructed molecular imaging probe exhibits excellent tumor tissue targeting and enrichment capabilities, superior biocompatibility, and clear imaging contrast, enabling specific recognition of PD-L1-positive tumor lesions. It can be applied to clinical scenarios such as early precision diagnosis of tumors, screening of patients for immunotherapy, real-time monitoring of efficacy, and prognostic assessment, possessing significant translational medical value and broad application prospects.

This invention discloses a heterocyclic polyarylamide-polyhydroxyarylamide precipitated fiber, its preparation method, and a heterocyclic polyarylamide-polybenzodioxazole paper, belonging to the technical field of high-performance organic fiber and paper preparation. The preparation method of the precipitated fiber includes the following steps: A. Preparation of the polymerization solution: 3,3'-dihydroxybenzyl diamine (DHB) and 2-(4-aminophenyl)-5-aminobenzimidazole (DAPBI) are dissolved in an organic solvent containing a solubilizing salt, and then terephthaloyl chloride is added to react and obtain a polymer solution with a solid content of 1-10%; B. Precipitation: After degassing, filtering, metering, and spinning, the polymer solution is mixed with a coagulation bath and precipitated to obtain the heterocyclic polyarylamide-polyhydroxyarylamide precipitated fiber. A heterocyclic polyarylamide-polybenzodioxazole paper prepared from this precipitated fiber is also provided. This invention introduces a heterocyclic polyaromatic amide structure through copolymerization, which not only improves the strength and composite properties of the cyclized PBO paper, but also maintains a decomposition temperature comparable to that of pure PBO fiber, exceeding 600℃, demonstrating excellent thermal stability. Furthermore, this method is simple and easy to implement, making it suitable for industrial production applications.

This invention discloses a lutein composition for relieving eye fatigue and its preparation method, belonging to the field of functional foods. The process involves: reacting phycocyanin and kelp polyphenols to obtain modified phycocyanin; reacting fucoidan and β-glucuronidase to obtain modified fucose; reacting fish collagen peptides and zinc chloride to obtain collagen peptide-zinc chelate; reacting lutein silica cage complex and modified phycocyanin to obtain a first complex; reacting the first complex and modified fucose to obtain a second complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil to obtain a lutein complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil, then adding trehalose to obtain the lutein complex; and finally mixing the lutein complex with maltodextrin to obtain the lutein composition.

The application discloses a kind of polyamide composite material and its preparation method and application, it is related to the technical field of plastic.A kind of polyamide composite material includes the following weight parts of component: first PA resin 48-72 parts;Nylon composition 9-26 parts;Nylon composition includes second PA resin and glass fiber, the number ratio of glass fiber in nylon composition is 90-100% in the distribution of the retained length of 500 μm below, the number ratio of glass fiber retained length is 0-10% in the distribution of 500 μm above;The relative viscosity of first PA resin is not less than 2.7, and the relative viscosity of nylon composition is not less than 2.4.The polyamide composite material with excellent surface treatment performance and mechanical properties is prepared by adding nylon composition in first PA resin in the application, is suitable for being used for preparing automobile parts, especially suitable for functional parts needing surface treatment or using special appearance requirements.

This invention discloses an antimicrobial peptide, Cih1, and its applications. The amino acid sequence of antimicrobial peptide Cih1 is shown in SEQ ID NO.1. The antimicrobial peptide Cih1 provided by this invention is a cationic peptide, rich in cysteine ​​(-cys) residues, which can form an amphiphilic β-sheet secondary structure. This structure endows it with excellent antibacterial and antiviral activity, and can effectively inhibit the proliferation and invasion of pathogens in aquaculture water. Based on the broad-spectrum resistance of antimicrobial peptide Cih1 to pathogens, it can help aquatic animal hosts resist infection by various pathogens, and has broad application prospects in the field of anti-infective drug development for aquaculture.

The application discloses coumarin compounds, and a preparation method and application thereof. The coumarin compound is selected from a compound shown in formula (A-A); wherein R1 is selected from a group shown in the formula, H or C1-C10 alkyl, wherein R 1′ oxalyl chloride ester selected from H or C1-C10, R 1” substituted or unsubstituted aryl; R2 is selected from a group shown in the formula or H, wherein R 2′ substituted or unsubstituted aryl; R3 is selected from a group shown in the formula, H or hydroxyl, wherein R 3′ C1-C10 carboxylic acid, R 3” substituted or unsubstituted aryl; and when R1 of the coumarin compound is selected from hydrogen, R3 cannot be selected from hydroxyl. The coumarin compound has simple structure, small molecular weight and good biological activity, can inhibit test bacteria in different degrees, and can be used as a potential new antibacterial agent.

The application belongs to the technical field of biology and relates to an anti-OX40L nanobody and application thereof. The anti-OX40L nanobody comprises a heavy chain variable region, the amino acid sequence of which is shown as SEQ ID NO:1, and the amino acid sequences of the complementarity determining regions CDR1, CDR2 and CDR3 thereof are shown as SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5 respectively. The application obtains the nanobody targeting OX40L from a llama nanobody natural library through phage display technology, does not need animal immunization, and the obtained antibody can specifically recognize Human OX40L recombinant protein, HEK293 hOX40L overexpression cells and CHO-K1 cynoOX40L overexpression cells, and exhibits good species cross-reactivity and binding activity. Based on the blocking potential of the nanobody on the OX40 / OX40L signal pathway, the nanobody shows a broad prospect in treating inflammatory and autoimmune diseases.

This invention provides an aptamer targeting the growth hormone receptor and its uses, the nucleotide sequence of which is shown in SEQ ID NO.1 or SEQ ID NO.2. This invention utilizes a modified SELEX technique to perform multiple rounds of screening targeting recombinant GHR protein, obtaining specific nucleic acid aptamers with high affinity for GHR (KD values ​​at the micromolar level). In vivo distribution experiments show that the fluorescently labeled aptamer can efficiently and specifically accumulate in white adipose tissue (including inguinal and gonadal fat) at the in vivo level, exhibiting excellent tissue targeting. The nucleic acid aptamer and its derivatives of this invention can be used to prepare drugs or diagnostic reagents targeting adipose tissue, for example, for the prevention or treatment of metabolic diseases such as obesity, type 2 diabetes, fatty liver, hyperlipidemia, or cardiovascular disease.

This invention discloses an anti-Dabie Banda virus Gn protein nanobody, a bispecific antibody, and their applications, belonging to the field of biodetection technology. This invention constructs an alpaca natural nanobody library, using recombinantly expressed Gn protein as the antigen. Through multiple rounds of gradient screening using phage display technology, three nanobodies—G15, G20, and G40—that specifically bind to the Dabie Banda virus Gn protein are obtained. All three antibodies exhibit excellent antigen-binding specificity and virus-neutralizing activity; among them, G40 shows the most prominent neutralizing effect. The bispecific antibody G40-C1q, constructed based on G40, combines Gn protein and human C1q protein, achieving a synergistic effect of antiviral activity and inhibition of inflammatory damage. It can be used for the development of drugs for the prevention and treatment of Dabie Banda virus infection and for high-specificity detection reagents, providing candidate antibody drugs for the treatment of fever with thrombocytopenia syndrome, and providing a reliable tool for virus-related research, disease diagnosis, and clinical translation.

This application relates to the field of biomedicine, specifically to the use of small molecule compounds in the preparation of drugs for the treatment and / or prevention of α-synuclein diseases, wherein the small molecule compounds are selected from at least one of AO-365 / 43264238 and AG-670 / 40728295. The small molecule compounds provided in this invention can precisely target and interfere with α-syn-VAPB interactions, improving neuronal damage caused by abnormal aggregation of α-synuclein, thereby safely and effectively treating and / or preventing α-synuclein diseases, particularly Parkinson's disease.

The application discloses a donkey skin oligopeptide, and an application thereof. The amino acid sequence of the donkey skin oligopeptide is selected from Glu-Thr-Trp-Arg or Ala-Tyr-Phe-Pro-Lys. The donkey skin oligopeptide disclosed by the application has alpha-glucosidase and alpha-amylase inhibitory activity, can assist in reducing blood sugar, has very important significance for developing medicines with blood sugar reducing function, and can be used for preparing alpha-glucosidase, alpha-amylase inhibitors and anti-type 2 diabetes drugs.

This invention belongs to the field of polyurethane technology, specifically relating to rigid polyurethane foam materials for high-temperature resistant pipelines and their preparation method. The polyurethane foam is made from component A and component B in a mass ratio of 100:(130~150). Component A includes the following raw materials by mass percentage: polyether polyol A: 7~36%; polyether polyol B: 13~35%; polyether polyol C: 13~37%; catalyst: 3~8%; additives: 3~14%; dispersant: 0.3~4.2%; foam stabilizer: 1.3~2.5%; pentane blowing agent: 6~15%. Component B is polymethylene polyphenyl isocyanate. By using specific polyether blends, adding nano-zirconia and dispersants, and optimizing the ratio of component A to component B, the high-temperature resistance, compressive strength, and dimensional stability of the polyurethane foam are significantly improved, making it suitable for the preparation of pipeline insulation materials.

This invention provides a nucleic acid aptamer that binds to the Spike protein of the novel coronavirus SARS-CoV-2 and its applications. The nucleic acid aptamer includes the nucleotide sequence shown in SEQ ID NO 1, 2, or 3; the nucleic acid aptamer, its combinations, and derivatives can specifically bind to the Spike protein of SARS-CoV-2 (wild-type or mutant), blocking the interaction between the Spike protein and the TLR4 protein, and inhibiting the inflammatory response triggered by SARS-CoV-2 virus infection; it has advantages such as high affinity, strong stability, small molecular weight, and low immunogenicity; this invention can be applied in the fields of detection, labeling, imaging, diagnosis, and treatment of the novel coronavirus.

This invention discloses a pea peptide, its preparation method, and its application, belonging to the technical field of plant polypeptide preparation and application. A mixture of sodium bicarbonate and sodium alginate is sprayed onto pea protein powder, and then dried at 70-80℃ for 3-5 hours. The dried pea protein powder is mixed with water to obtain a mixed solution, which is then heated to 45-55℃. Alkaline protease is added for enzymatic hydrolysis, and papain is added to the hydrolysate for further hydrolysis. After hydrolysis, enzyme inactivation is performed. The hydrolysate is filtered, concentrated, and spray-dried to obtain the pea peptide. The pea peptide prepared by this invention has a high recovery rate, low free amino acid content, and more than 85% of the molecules with a molecular weight less than 600 Da. It also exhibits high enzymatic hydrolysis efficiency, short hydrolysis time, and good antioxidant activity.

This invention discloses an in-situ depolymerization modification composition of mineral-derived humic acid, its method, and its applications. The composition comprises a hydrogen bond acceptor and an acidic hydrogen bond donor, wherein the hydrogen bond acceptor is selected from one or more of choline compounds, betaine compounds, and quaternary ammonium salt compounds; and the acidic hydrogen bond donor is an aliphatic polycarboxylic acid or a hydroxycarboxylic acid. This method is simple, green, and efficient, yielding a product with low molecular weight, good water solubility, and strong resistance to hard water. It is suitable for various product forms such as solid fertilizers, liquid fertigation fertilizers, and soil conditioners, and has broad application prospects in modern agriculture and ecological restoration.

This invention discloses a small molecule peptide derived from *Hirudo medicinalis*, with the amino acid sequence Leu-Leu-Phe-Arg. The peptide was obtained by enzymatically hydrolyzing the waste protein residue after purifying hirudin from *Hirudo medicinalis*, combined with tyrosinase-functionalized magnetic bead affinity fishing technology and mass spectrometry identification. In vitro kinetics and molecular dynamics simulations confirmed that the peptide LLFR significantly prolongs the lag time of monophenolase reactions. It penetrates the catalytic pocket of tyrosinase through an "induced fit" mechanism, forming a stable dead-end complex with the enzyme and inducing the unfolding of the enzyme's tertiary structure. Cell experiments confirmed that LLFR significantly inhibits tyrosinase activity and melanin synthesis in melanoma cells without significant cytotoxicity. This peptide exhibits high safety and good water solubility, providing a new approach for the high-value utilization of industrial waste from *Hirudo medicinalis*, and is suitable for the industrial production of whitening cosmetics and functional products.

This invention belongs to the field of biomedical technology and discloses the application of the small molecule tripeptide Ser-Ser-Cys (SSC) in inhibiting the phenotypic transformation of vascular smooth muscle cells (VSMCs). SSC is an endogenous differentially expressed tripeptide screened and identified from the serum of hypertensive non-atherosclerotic patients using non-targeted metabolomics technology. In vitro experiments show that SSC can effectively inhibit angiotensin II (Ang II)-induced abnormal proliferation, migration, and phenotypic transformation from contractile to synthetic VSMCs. Its mechanism of action is related to the regulation of the Keap1 / Nrf2 signaling pathway, improvement of cellular oxidative stress, and mitochondrial function. This invention provides a new approach for using SSC in the preparation of drugs or functional foods for the prevention and treatment of hypertension-related vascular remodeling.

This invention belongs to the field of biomedical technology and relates to a polypeptide targeting cGAS and its applications. The polypeptide comprises an active peptide and a membrane-penetrating peptide, the membrane-penetrating peptide being fused to the N-terminus of the active peptide. The amino acid sequence of the active peptide is identical to amino acid regions 45-63 of human IκB kinase-binding protein (IKIP) or 43-60 of mouse IKIP. The polypeptide provided by this invention can enter cells, specifically bind to cGAS, inhibit cGAS aggregation and phase separation, thereby inhibiting cGAS enzyme activity, reducing type I interferon expression, maintaining antiviral immune homeostasis, and providing a new candidate strategy for the clinical treatment of viral infections and autoimmune diseases.

The present invention relates to the technical field of recombinant protein design and synthesis, and specifically discloses a recombinant spider silk protein and its synthesis method. The recombinant spider silk protein provided by the present invention undergoes optimized design such as replacement of the N-terminal and C-terminal domains of different spider species and different spider silk types, and large fragment recombination of the middle functional domain repeat sequences. A series of recombinant spider silk proteins are obtained through cell-free protein synthesis methods and prokaryotic fermentation expression. Compared with natural spider dragline silk proteins or other existing recombinant spider silk proteins, they generally have good composite mechanical properties and high-temperature resistance characteristics. At the same time, they have a relatively small molecular weight, significantly improving the total expression level and soluble expression, providing new raw materials and design and synthesis methods for the development of high-performance biomaterials.

This invention belongs to the field of bioengineering technology, specifically relating to the preparation and application of colloidal gold immunochromatographic assay based on AMH nanobodies. This invention discloses two AMH nanobodies, the amino acid sequences of which are shown in SEQ ID NO.1 and SEQ ID NO.2, and the nucleotide molecules encoding these amino acid sequences are shown in SEQ ID NO.3 and SEQ ID NO.4. This invention also discloses a method for preparing colloidal gold immunochromatographic assay based on AMH nanobodies. The results of the examples show that the molecular weight of both AMH nanobodies is approximately 15 kDa, consistent with the size of nanobodies. The purified nanobodies can be used for the preparation of colloidal gold immunochromatographic assay, and the colloidal gold can be used for the detection of AMH in the serum of sheep, donkeys, horses, and camels.

This invention discloses a PBP peptide that specifically binds to PD-1, its screening method, and its applications. The PBP peptide has the sequence shown in SEQ ID No. 1. This invention uses bacterial surface display technology to screen for PD-1-targeting PBP peptides. These peptides specifically bind to PD-1, exhibit cross-reactivity with human and mouse PD-1, and can competitively bind to PD-1 with PD-L1, effectively blocking the interaction between PD-1 and PD-L1, reversing the function of exhausted T cells, and exerting an anti-tumor effect. Compared to antibodies, the PBP peptide obtained in this invention has a small molecular weight, strong penetration ability at tumor sites, low production cost, simple preparation process, and does not cause serious immune-related side effects. While achieving corresponding therapeutic effects, it avoids the disadvantages of antibody drugs, providing a low-molecular-weight candidate drug for tumor immunotherapy.