69 results about "Plant nodule" patented technology

The invention provides application of GmEDS5a and / or GmEDS5b genes in regulation and control of soybean root nodule size and soybean nitrogenase activity or in soybean breeding, and belongs to the technical field of gene engineering. The GmEDS5a and / or GmEDS5b genes are / is knocked out, the proportion of root nodules with the particle size larger than 2 mm is increased, the soybean nitrogenase activity is remarkably improved, symbiotic nitrogen fixation is promoted, and great significance is achieved for increasing the soybean yield.

The invention discloses a gene GmSCAMP3 for regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules and application of the gene GmSCAMP3. Specifically, the invention provides a protein for regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules, and the amino acid sequence of the protein is as shown in SEQ ID NO: 2. The invention also provides a method for improving soybean yield or changing soybean root nodule symbiotic nitrogen fixation capacity. The method comprises the step of introducing the polynucleotide encoding the protein into soybean cells or tissues to obtain transgenic soybeans. The invention finds that the nitrogenase activity of the Gmscamp3 mutant root nodule is only 65.79% of that of a wild type root nodule, which indicates that the GmSCAMP3 protein plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of the soybean root nodule.

The invention belongs to the technical field of plant genetic engineering and soybean breeding, and discloses application of a GmCDPKb gene in regulation and control of the number of soybean symbiotic nodules, the nucleotide sequence of the GmCDPKb gene is shown as SEQ ID NO.1, and the amino acid sequence of encoded protein is shown as SEQ ID NO.2. According to the invention, a GmCDPKb gene mutation and overexpression soybean stable genetic transformation plant is constructed, and character investigation shows that the nodulation number of a mutant plant is obviously lower than that of a wild type, and the nodulation number of an overexpression plant is obviously higher than that of the wild type. The result shows that the GmCDPKb gene plays a role in regulating and controlling the number of soybean nodules. The invention reveals that the GmCDPKb gene is a key regulatory factor for regulating the number of soybean root nodules symbiotic nodules, the number of soybean nodules can be stably and obviously regulated by regulating the expression quantity of the gene, and important gene resources and germplasm materials are provided for analyzing a soybean symbiotic nitrogen fixation molecular mechanism and cultivating a new efficient nitrogen fixation soybean variety.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention relates to the technical field of biology, and discloses application of PHR2 in regulating nodulation and nitrogen fixation processes of leguminous plants under phosphorus starvation stress, and the application is any one of the following applications: P1, application in enhancing nitrogen fixation capability of the leguminous plants under the phosphorus starvation stress; p2, application in promoting formation of root nodules with nitrogen fixation capacity of leguminous plants under phosphorus starvation stress; p3, application of improving activity of nitrogenase in leguminous plant root nodules under phosphorus starvation stress; p4, application in leguminous plant breeding. The PHR2 protein and related biological materials thereof can be used for regulating and controlling the nitrogen fixation capacity of leguminous plants under phosphorus starvation stress.

The present application relates to a gene GmPMT for regulating soybean root nodule symbiotic nitrogen fixation and application thereof. Specifically, the present application relates to an isolated gene for regulating soybean root nodule symbiotic nitrogen fixation or nodule number, the coding region sequence of the gene is shown as SEQ ID NO:1 or a homologous sequence thereof has one or more substitutions, deletions and / or insertions of nucleotides compared with the sequence shown as SEQ ID NO:1. The present application also provides the use of the gene as described above in breeding soybeans with increased yield or changed nodule number, optionally, the changed nodule number is manifested as increased nodule number, nodule number per root and / or nodule number per unit root length, optionally, the changed nodule number is manifested as decreased nodule number, nodule number per root and / or nodule number per unit root length. The present application has great theoretical and application value for regulating soybean root nodule symbiotic nitrogen fixation or nodule number and its related application research.

The invention belongs to the technical field of agricultural biology, and provides a soybean nodulation and nitrogen fixation related gene and application of an encoding protein thereof, the nucleotide sequence of the gene Gm02g072800 is shown as SEQ ID NO: 1, or a variant sequence which encodes the same functional protein and has homology greater than or equal to 90%; the amino acid sequence of the protein coded by the gene Gm02g072800 comprises a conservative AP2 structural domain, belongs to an ERF transcription factor family, is positioned in a cell nucleus, and has the functions of promoting soybean root nodule development and symbiotic nitrogen fixation. According to the invention, the specific expression of the gene in the soybean middle column and root nodule endodermis is determined, and a target gene is provided for cultivating soybean varieties with high nitrogen fixation efficiency.

The invention discloses application of a GmNSE1 gene in regulation and control of soybean root nodule nitrogen fixation efficiency. The application of the GmNSE1 gene or a biological material related to the GmNSE1 gene in at least one of the following aspects: A1) regulating and controlling the nitrogen fixation efficiency of soybean root nodules or preparing a product for regulating and controlling the nitrogen fixation efficiency of the soybean root nodules; a2) cultivating soybeans with improved nitrogen fixation efficiency or preparing products for improving the nitrogen fixation efficiency of the soybeans; a3) preparing transgenic soybeans; the nucleotide sequence of the GmNSE1 gene is as shown in SEQ ID NO. 1. The specificity of the GmNSE1 gene is expressed in a root nodule infection area, the GmNSE1 gene has E3 ubiquitin ligase enzyme activity in vitro, and compared with a control group, the nitrogenase activity of a soybean plant with the overexpressed GmNSE1 gene is remarkably improved, which shows that the GmNSE1 gene plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules, and the GmNSE1 gene has a good application prospect in the field of soybean root nodule symbiotic nitrogen fixation. The method has application prospects in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

The invention relates to the technical field of gene engineering, and discloses an application of a Glyma. 11G085500 or Glyma. 01G159200 gene in regulation and control of soybean nodulation. The gene Glyma. 11G085500 and the gene Glyma. 01G159200 are a group of homologous genes in the soybean, and the gene Glyma. 11G085500 and the gene Glyma. It is found for the first time that the group of homologous genes can regulate and control the number of soybean root nodules, new targets and methods are provided for soybean breeding and construction of related research models, and important scientific research and practical significance is achieved.

The present invention relates to a gene GmHB21 involved in soybean nodule vascular bundle development and its use. Specifically, the present invention relates to an isolated gene involved in soybean nodule vascular bundle development or regulating the number of soybean nodules, wherein the coding region sequence of the gene is shown in SEQ ID NO: 1 or a homologous sequence thereof. The present invention also provides a method for regulating the number of plant nodules, comprising introducing the gene described above, or a sequence that inhibits the expression of the gene described above, into target plant cells or tissues to obtain a transgenic plant, wherein the number of nodules in the transgenic plant is altered compared to the target plant, and the plant is a legume, preferably soybean. In addition, the present invention also provides the use of the gene described above, or the sequence that inhibits the expression of the gene described above, in cultivating legumes, such as soybeans, with altered nodule numbers.

The application belongs to the field of bioengineering and particularly relates to a Bradyrhizobium elkanii with improved nodulation capacity and a construction method thereof. The pip gene of the Bradyrhizobium elkanii FG-486 is knocked out to improve the nodulation capacity of the Bradyrhizobium elkanii FG-486, and the Bradyrhizobium elkanii FG-486 delta P with improved nodulation capacity is obtained. Compared with the wild strain FG-486, the average nodulation number of soybean plants after seed dressing with the Bradyrhizobium elkanii FG-486 delta P is increased by 31.15%, the root nodule dry weight is increased by 10.26%, and the soybean yield is increased by 7.85%.

The invention discloses a planting method for sowing astragalus sinicus synchronously with rice fertilization, and belongs to the technical field of agricultural cultivation. Aiming at the defects of low emergence rate and tedious independent operation of the existing astragalus sinicus seeding, the invention provides the following steps: preparing a granular fertilizer to be applied and astragalus sinicus seeds, and carrying out physical dry mixing in an anhydrous state before application to form a uniform seed-fertilizer mixture; before the rice growth period is in the ear differentiation II period, the rice field is adjusted to keep a shallow water layer state or a wet state; and uniformly spreading the seed-fertilizer mixture into the rice field at one time along the rice planting row. According to the method, the habitat conditions in the middle and early stage of rice growth are utilized, granular fertilizer is used as a physical dispersion carrier of seeds, rice fertilization and astragalus sinicus seeding are combined into one operation, the germination problem caused by soil drought in traditional late seeding is solved, the growth period is prolonged, root nodulation and nitrogen fixation are promoted, and the yield is increased. The cultivation effects of saving labor and cost and being light and simplified are achieved.

The invention discloses application of a GmNSE1 gene in regulation and control of soybean root nodule nitrogen fixation efficiency. The application of the GmNSE1 gene or a biological material related to the GmNSE1 gene in at least one of the following aspects: A1) regulating and controlling the nitrogen fixation efficiency of soybean root nodules or preparing a product for regulating and controlling the nitrogen fixation efficiency of the soybean root nodules; a2) cultivating soybeans with improved nitrogen fixation efficiency or preparing products for improving the nitrogen fixation efficiency of the soybeans; a3) preparing transgenic soybeans; the nucleotide sequence of the GmNSE1 gene is as shown in SEQ ID NO. 1. The specificity of the GmNSE1 gene is expressed in a root nodule infection area, the GmNSE1 gene has E3 ubiquitin ligase enzyme activity in vitro, and the activity of a mutant with the GmNSE1 gene knocked out is remarkably reduced compared with that of wild nitrogenase, so that the GmNSE1 gene plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules; the method has application prospects in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

This application provides the use of the MsGRF1c gene in regulating alfalfa nodule senescence, nitrogen fixation efficiency, and biomass. This application demonstrates that the MsGRF1c gene can delay alfalfa nodule senescence, improve nodule nitrogen fixation efficiency, and thereby increase plant biomass under low-nitrogen conditions. This new discovery complements the regulatory mechanism for nodule senescence in legumes and has important utility in reducing nitrogen fertilizer application.

The invention relates to the technical field of biology, in particular to a sweet clover MaWRKY76 gene and application thereof.The sweet clover MaWRKY76 gene is cloned firstly, and the expression mode of the sweet clover MaWRKY76 gene under the salt stress condition is analyzed through real-time quantitative PCR; by constructing a yeast heterologous expression vector, the MaWRKY76 is proved to be capable of remarkably improving the salt tolerance of yeast cells. A plant overexpression and RNAi vector is further constructed, sweet clover hairy roots are transformed, and the result shows that the MaWRKY76 not only participates in regulating the symbiotic process of root nodules, but also plays a key role in resisting salt stress. The genetic strategy based on MaWRKY76 can effectively improve symbiotic nitrogen fixation efficiency, plant sustainability and yield of sweet clover under salt stress, provides a theoretical basis for efficient nodulation and nitrogen fixation of leguminous plants under adversity stress, and has positive significance in promoting green sustainable agricultural development.

This invention belongs to the field of microbial technology and discloses a synthetic microbial community for enhancing biological nitrogen fixation in karst grasslands, its construction method, and its application. The synthetic microbial community includes... Sinorhizobium americanum , Sionrhizobium sp. as well as Bacillus cereus Wherein: the Sinorhizobium americanum The accession number is GDMCC NO: 67796; Sionrhizobium sp. The accession number is GDMCC NO: 67797; Bacillus cereus The accession number is GDMCC NO: 67798. For leguminous forage grasses that require repeated sowing, the seeds are treated with a synthetic microbial community before being sown in the field; for perennial leguminous forage grasses that have already been planted, the synthetic microbial community is diluted with water and sprayed onto the surface of adjacent leguminous forage grasses. This invention screens out suitable and efficient root nodule endophytic bacteria in karst areas and constructs a synthetic microbial community to enhance the biological nitrogen fixation of leguminous forage grasses. It clarifies the technical details of the practical application of the synthetic microbial community in the field. The application of this technology can significantly improve the biological nitrogen fixation rate of leguminous forage grasses, significantly reduce the amount of nitrogen fertilizer used in forage grasses, and significantly increase forage grass yield.

The invention discloses a method for efficiently screening rhizobium with functions of promoting growth and inhibiting aspergillus flavus and application of the method, the field ecological pre-enrichment effect of a microbial agent with an ARC function is introduced into a rhizobium screening process for the first time, and the abundance of a target flora is directionally increased from the source through the toxin control and bacterium enrichment effect of the rhizobium. The method comprises the following steps: synchronously collecting rhizosphere soil, root system and root nodule samples, and comprehensively separating rhizobium; a high-throughput primary screening system with parallel growth-promoting and antibacterial functions is constructed, and the nitrogen-fixing growth-promoting capability and the antibacterial effect of volatile substances of candidate strains are synchronously evaluated in an early stage through a pot experiment and a double-vessel buckling method. And further performing functional quantification, multi-gene phylogenetic identification and omics mechanism analysis on the positive strain. The method overcomes the defects that a traditional method is single in function and low in efficiency, and an effective way for rapidly, directionally and systematically screening multifunctional rhizobium resources from an optimized rhizosphere ecosystem is provided.

Rhizobial infection is the key process for initiating symbiotic nitrogen-fixing root nodules in legumes, which requires specific recognition of signal molecules produced by the bacteria and their hosts. Here, it is established that rhizobial tRNA-derived small RNA fragments (tRFs) are crucial signal molecules modulating host nodulation, which uncovers a bacterial small RNA-mediated mechanism for prokaryote-eukaryote interaction. Transgenic plants are also provided that express a construct encoding rhizobial-derived tRNA, which is subsequently cleaved to produce artificial tRFs. Constructs and methods of producing the same are also provided, as well as modifications for repressing a mechanism for the negative regulation of nodulation present within plants.

This invention belongs to the field of genetic engineering technology, specifically relating to soybeans. GmPLAT5 Application of genes in promoting root nodule formation in legumes. This invention utilizes the pZP211 overexpression vector to rapidly obtain transgenic plants through Agrobacterium-mediated genetic transformation of soybean, successfully... GmPLAT5 Overexpression was performed, and the number of root nodules in chimeric transgenic plants was counted. The results showed that... GmPLAT5 The gene can positively regulate the number of soybean root nodules with significant effects. This invention is the first to propose Gm. PLAT5 It can positively regulate the nodulation process in soybeans and has proven that soybeans GmPLAT5 Overexpression of the gene under both water treatment and salt stress conditions can significantly increase the number of soybean root nodules, providing important evidence for further analysis of its salt tolerance molecular mechanism. In the future, it can be applied to soybean production and molecular breeding through transgenic and molecular marker methods, which has great application value.

The invention relates to methods for modulating the symbiotic relationship between plants, for example legumes, and nitrogen fixing bacteria in the root nodules. The invention also relates to modified plants, for example gene edited plants that have an altered symbiotic relationship with nitrogen fixing bacteria in the root nodules.

The invention provides rhizobium japonicum GY4 and application thereof, and relates to the technical field of microorganisms. Comprising soybean rhizobium GY4, the classification name of the soybean rhizobium GY4 is Rhizobium sp, the soybean rhizobium GY4 is preserved in Guangdong Microbial Culture Collection Center on July 15, 2025, the preservation number is GDMCC No. 66686, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The strain belongs to the rhizobium, can significantly improve the abundance of the rhizobium, promote the formation and development of root nodules, increase the number of the root nodules, improve the nitrogen fixation capability of the soybeans, and efficiently promote the growth of the soybeans under the strip relay intercropping condition of the soybeans and the corn, and provides a new strain resource for improving the nitrogen fixation efficiency in the soybean production.

The invention relates to an application method of a special microbial agent for improving the nitrogen fixation efficiency of soybean root nodules, and belongs to the field of agricultural microorganism application. A core strategy of staged time sequence application is adopted: firstly, before sowing or during sowing, a first complex microbial inoculant containing proline secreting bacteria and indigenous infectious microbe inhibiting bacteria is applied into soil, and a pre-culture period is performed for 5-15 days; during the period, proline is converted into indole-3-acetic acid (IAA) by a fourth microorganism which synchronously exists or is introduced, so that a growth-promoting microenvironment is established at the rhizosphere, and meanwhile, a third microorganism inhibits harmful infectious microbes; next, bradyrhizobium or sinorhizobium is applied to the seedlings or rhizosphere. According to the time sequence design, an optimized colonization ecological niche with low competition and high chemotactic signals is created for target rhizobium through a synergistic mechanism of firstly improving the environment and then introducing strains. According to the method, the infection success rate of rhizobium can be remarkably increased, the number of root nodules and nitrogenase activity are increased, soybean root development is synchronously promoted, and the soybean symbiotic nitrogen fixation efficiency and yield are improved.

This invention belongs to the field of agricultural technology, specifically relating to a method for recommending nitrogen fertilizer for peanuts that coordinates yield and biological nitrogen fixation. The method includes determining the agronomic critical nitrogen application rate based on peanut yield and a model showing the relationship between nitrogen application rate and peanut yield; determining the nitrogen inhibition threshold based on peanut root nodule weight, root nodule nitrogenase capacity, and a model showing the relationship between nitrogen application rate and root nodule weight and root nodule nitrogen fixation capacity; and determining the nitrogen fertilizer application rate that is conducive to yield formation and fully utilizing root nodule nitrogen fixation capacity based on the agronomic critical nitrogen application rate and the nitrogen inhibition threshold. This method coordinates yield and biological nitrogen fixation, minimizing problems caused by excessive nitrogen fertilizer application, such as inhibited root nodule nitrogen fixation, decreased nitrogen use efficiency, increased production costs, and environmental pollution.

The invention relates to a soybean root nodule endogenous fomesafen degrading bacterium and application thereof. The classification name of the fomesafen degrading bacterium is Mycobacterium sp. W109, and the preservation number of the fomesafen degrading bacterium is CCTCC (China Center for Type Culture Collection) NO: M 20251729. According to the invention, a fomesafen degrading bacterium is separated from soybean root nodules, the bacterium can stably degrade fomesafen, and the variety of the fomesafen degrading bacterium is expanded.

The invention provides application of a GmUOX1a gene in increasing the number of root nodules of leguminous plants, and belongs to the technical field of gene engineering. The number of root nodules of leguminous plants is increased through overexpression of the GmUOX1a gene. The functional effect of the soybean GmUOX1a gene in the nodulation regulation process is confirmed for the first time. The over-expression vector of the GmUOX1a gene is used for carrying out genetic transformation on a receptor soybean plant, so that the number of root nodules of the transgenic soybean plant is remarkably increased, the symbiotic nitrogen fixation efficiency is promoted, and the method has important significance on increasing the soybean yield.

The application discloses a Neorhizobium rhizobium strain isolated and purified from soybean root nodules and application thereof. The strain is a new species of the Neorhizobium genus through physiological and biochemical determination and phylogenetic analysis, and is named as Neorhizobium glycine EC2-8. The strain re-inoculation experiment shows that the rhizobium strain can invade the soybean roots and form effective nodulation, and can promote the growth of soybeans.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a soybean GmNEN5 gene to regulation and control of plant root nodule nitrogen fixation efficiency and delay of root nodule senescence. The soybean GmNEN5 gene is proved to be a positive regulatory factor for regulating the development of the root nodule and regulating the nitrogen fixation efficiency of the root nodule by overexpressing a coding gene of the soybean GmNEN5 protein in the soybean root nodule, and the condition that the root nodule senescence can be delayed and the nitrogen fixation capability of the plant can be improved by overexpressing the GmNEN5 gene in the soybean is found; the method has a good application prospect in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

The invention provides a microorganism for improving grassland and application of the microorganism for improving the grassland, the microorganism for improving the grassland comprises Bradyrhizobium japonicum TG101, and further comprises one or more of Brevibacterium tumefaciens TG15, Phyllobacterium tumefaciens TG47 and Enterobacter ludwigii DP20, and the microorganism for improving the grassland further comprises one or more of Bradyrhizobium japonicum TG101, the Brevibacterium tumefaciens TG15, the Phyllobacterium tumefaciens TG47 and the Enterobacter ludwigii DP20. According to the application, novel application of the brevibacterium friariorum TG15, the leaf bacillus dulcis TG47 and the enterobacter ludwigii DP20 is innovatively found, that is, the three bacteria and the combination thereof can promote nodulation of the bradyrhizobium elwardii TG101; the compound microbial fertilizer disclosed by the invention has the functions of greatly improving the nodulation quantity, root nodule diameter and nitrogen fixation capability of lespedeza daurica in the field, and greatly improving the yield, quality and medicinal components of lespedeza daurica.

The present invention discloses the application of GmAIR12-2 protein in improving the ability of legumes to tolerate low-phosphorus stress. The present invention finds that under low-phosphorus stress, overexpression of GmAIR12-2 protein in soybean plants can improve the root growth of soybean plants, increase the number of small root nodules and the fresh weight of small root nodules of soybean plants, increase the above-ground parts and root dry weight of soybean plants, increase total root length and root surface area, indicating that the protein can improve the tolerance of soybeans to low-phosphorus stress and reduce the adverse effects of low-phosphorus stress on soybean growth. Thus, the present invention provides the application of GmAIR12-2 protein in improving the ability of legumes to tolerate low-phosphorus stress, and the protein can also be used to cultivate transgenic legume varieties resistant to low-phosphorus stress, so that legumes planted in soils with lower available phosphorus content can also grow normally, thereby increasing the yield of legumes. At the same time, the present invention also provides important gene resources for breeding legume varieties resistant to low-phosphorus stress.

The invention discloses bacillus velezensis and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis provided by the invention is bacillus velezensis, the strain number of the bacillus velezensis is 41S2, and the registration number of the bacillus velezensis in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.35218. The bacillus velezensis provided by the invention is a bacillus velezensis strain. The bacillus velezensis 41S2 provided by the invention has a remarkable growth promoting and yield increasing effect under the condition of saline-alkali stress, and the plant height, the biomass, the root nodule forming ability, the pod number of a single plant and the grain yield of soybeans are remarkably improved; the strain 41S2 can also improve the quality of soybean seeds under the saline-alkali stress condition, including the total carbon content and the total nitrogen content of the soybean seeds are increased and reach 1.77 times and 1.80 times of those of a control group respectively, and the soluble protein content and the isoflavone content of the soybean seeds are increased and reach 1.67 times and 1.91 times of those of the control group respectively.