112 results about "Plant nodule" patented technology

The invention discloses a soybean nodulation number related major single nucleotide polymorphism (SNP) site and application thereof, the SNP site is positioned on a soybean chromosome 2, the physical distance of the SNP site in a soybean genome version number Glycine max Wm82.a2.v1 is Chr0243129492, the SNP site corresponds to a 20th basic group of a sequence shown as SEQ ID NO.1, when the site is T / T homozygosis, the corresponding genotype is A, when the site is T / T homozygosis, the corresponding genotype is B, and when the site is T / T homozygosis, the corresponding genotype is C; when the site is C / C homozygous, the corresponding genotype is B; the number of root nodules is as follows: the genotype A homozygous soybean is greater than or candidate to be greater than that of the genotype B homozygous soybean. By detecting the SNP, a tested soybean population can be accurately divided into a low root nodule number (C / C) and a high root nodule number (T / T). Therefore, the molecular marker can be used as a basis for preliminarily identifying the number of soybean nodules, has high efficiency and applicability such as simplicity in operation, low cost and short consumed time, and has important values for digging germplasm of high-nodulation soybeans, solving the problem of nitrogen fertilizer abuse and promoting sustainable development of agriculture.

The invention discloses a CAPE polypeptide released by a protein coded by a soybean disease course related gene GmPR1-6 and application of the CAPE polypeptide. The nucleotide sequence of the soybean disease course related gene is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the soybean disease course related gene is as shown in SEQ ID NO.2, and the sequence of CAPE polypeptide released at the C terminal of the protein is as shown in SEQ ID NO.9. The soybean disease course related gene GmPR1-6 provided by the invention is a gene coding a core protein participating in soybean immune defense reaction, the C tail end of the GmPR1-6 protein can be sheared to release CAPE polypeptide, the CAPE polypeptide is externally applied to weaken early immune defense reaction triggered by soybean during infection of rhizobium, the weight and size of a single root nodule are increased, and the disease resistance of the soybean is improved. The symbiotic nodulation and nitrogen fixation capabilities of the soybeans are improved, and the method has potential application value in planting of leguminous plants.

The invention relates to a gene GmHB21 participating in development of vascular bundles of soybean root nodules and application of the gene GmHB21. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, the method comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, and the plant is a leguminous plant, preferably soybean. In addition, the invention also provides application of the gene or a sequence for inhibiting expression of the gene in cultivation of leguminous plants with changed nodulation number, such as soybean.

The invention belongs to the technical field of biology, and particularly relates to application of a soybean GmSRO5 gene in promoting generation of leguminous plant root nodules. A pZP211 overexpression vector is utilized, a transgenic plant is rapidly obtained through agrobacterium tumefaciens-mediated soybean genetic transformation, GmSRO5 is successfully overexpressed, root nodule number statistics is carried out on a chimera transgenic plant, and a result shows that the GmSRO5 gene can positively regulate and control the number of soybean root nodules and has a remarkable effect. The invention proposes that SRO5 can positively regulate and control the soybean nodulation process for the first time, and proves that the soybean GmSRO5 gene overexpression can significantly increase the number of soybean root nodules, and the soybean GmSRO5 gene overexpression can be applied to soybean production and molecular breeding through transgenosis, molecular marking and other means in the future, and has very important application value.

The invention provides a regional soil quality improvement method based on a crop rotation mode, and relates to the technical field of soil quality optimization, and the method comprises the following steps: S1, establishment of the crop rotation mode: establishing a crop rotation system with leguminous forage grass and gramineous crops as cores in a target area, the leguminous forage grass being medicago sativa, the gramineous crops being corn, and the gramineous crops being corn; a carousel or intercropping mode is adopted, the carousel mode is a period of 2-3 years, alfalfa is planted in the first year to the second year, and corn is planted in the third year; according to the intercropping mode, the corn and the alfalfa are planted according to the row ratio of 2: 1-3: 1, the row spacing of the corn ranges from 60 cm to 70 cm, and the row spacing of the alfalfa ranges from 30 cm to 35 cm. According to the method, alternate sowing and intercropping of the medicago sativa and the corn are combined, symbiotic nitrogen fixation of rhizobium of the medicago sativa and absorption of deep potassium by deep root systems are utilized, surface nutrients are utilized in cooperation with shallow root systems of the corn, a layered nutrient utilization mechanism of shallow nitrogen fixation and deep potassium release is formed, and compared with a traditional single cropping mode, the soil improvement quality is improved; the problems of regional surface soil nitrogen loss and low deep nutrient utilization rate are solved.

The invention provides a separated gene for regulating and controlling the nodulation number of leguminous plants. A coding region sequence of the gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 3 or a homologous sequence of the SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 3. The invention has great theoretical and application values for leguminous plant breeding and related application research thereof.

The invention relates to a gene GmPMT for regulating and controlling soybean root nodule symbiotic nitrogen fixation and application of the gene GmPMT. Specifically, the invention relates to a separated gene for regulating and controlling soybean root nodule symbiotic nitrogen fixation or nodulation number, a coding region sequence of the gene is shown as SEQ ID NO: 1, or a homologous sequence of the gene has substitution, deletion and / or insertion of one or more nucleotides compared with the sequence shown as SEQ ID NO: 1. The invention also provides application of the gene in cultivation of soybean with increased yield or changed nodulation number, optionally, the nodulation number change shows that the nodulation number, the nodulation number per root and / or the nodulation number per unit root length are increased, and optionally, the nodulation number change shows that the nodulation number per unit root length is increased. The change of the number of nodules is manifested as the number of nodules, the number of nodules per root and / or the number of nodules per unit root length is reduced. The invention has great theoretical and application values for adjusting the symbiotic nitrogen fixation or nodule number of soybean root nodules and related application research.

The invention provides application of GmEDS5a and / or GmEDS5b genes in regulation and control of soybean root nodule size and soybean nitrogenase activity or in soybean breeding, and belongs to the technical field of gene engineering. The GmEDS5a and / or GmEDS5b genes are / is knocked out, the proportion of root nodules with the particle size larger than 2 mm is increased, the soybean nitrogenase activity is remarkably improved, symbiotic nitrogen fixation is promoted, and great significance is achieved for increasing the soybean yield.

The invention discloses a gene GmSCAMP3 for regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules and application of the gene GmSCAMP3. Specifically, the invention provides a protein for regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules, and the amino acid sequence of the protein is as shown in SEQ ID NO: 2. The invention also provides a method for improving soybean yield or changing soybean root nodule symbiotic nitrogen fixation capacity. The method comprises the step of introducing the polynucleotide encoding the protein into soybean cells or tissues to obtain transgenic soybeans. The invention finds that the nitrogenase activity of the Gmscamp3 mutant root nodule is only 65.79% of that of a wild type root nodule, which indicates that the GmSCAMP3 protein plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of the soybean root nodule.

The invention belongs to the technical field of plant genetic engineering and soybean breeding, and discloses application of a GmCDPKb gene in regulation and control of the number of soybean symbiotic nodules, the nucleotide sequence of the GmCDPKb gene is shown as SEQ ID NO.1, and the amino acid sequence of encoded protein is shown as SEQ ID NO.2. According to the invention, a GmCDPKb gene mutation and overexpression soybean stable genetic transformation plant is constructed, and character investigation shows that the nodulation number of a mutant plant is obviously lower than that of a wild type, and the nodulation number of an overexpression plant is obviously higher than that of the wild type. The result shows that the GmCDPKb gene plays a role in regulating and controlling the number of soybean nodules. The invention reveals that the GmCDPKb gene is a key regulatory factor for regulating the number of soybean root nodules symbiotic nodules, the number of soybean nodules can be stably and obviously regulated by regulating the expression quantity of the gene, and important gene resources and germplasm materials are provided for analyzing a soybean symbiotic nitrogen fixation molecular mechanism and cultivating a new efficient nitrogen fixation soybean variety.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention relates to the technical field of gene engineering, in particular to application of a GmTGA1 L-a / b gene in increasing the number of soybean root nodules and in soybean breeding. The GmTGA1 L-a / b gene is applied to increase of the number of soybean root nodules or soybean breeding. The research finds that the knockout vector constructed by the invention is used for transforming a receptor soybean plant to obtain a transgenic plant, so that the number of soybean nodules can be remarkably increased, symbiotic nitrogen fixation is promoted, and the knockout vector has important significance on increasing the soybean yield.

The invention relates to the technical field of biology, and discloses application of PHR2 in regulating nodulation and nitrogen fixation processes of leguminous plants under phosphorus starvation stress, and the application is any one of the following applications: P1, application in enhancing nitrogen fixation capability of the leguminous plants under the phosphorus starvation stress; p2, application in promoting formation of root nodules with nitrogen fixation capacity of leguminous plants under phosphorus starvation stress; p3, application of improving activity of nitrogenase in leguminous plant root nodules under phosphorus starvation stress; p4, application in leguminous plant breeding. The PHR2 protein and related biological materials thereof can be used for regulating and controlling the nitrogen fixation capacity of leguminous plants under phosphorus starvation stress.

The present application relates to a gene GmPMT for regulating soybean root nodule symbiotic nitrogen fixation and application thereof. Specifically, the present application relates to an isolated gene for regulating soybean root nodule symbiotic nitrogen fixation or nodule number, the coding region sequence of the gene is shown as SEQ ID NO:1 or a homologous sequence thereof has one or more substitutions, deletions and / or insertions of nucleotides compared with the sequence shown as SEQ ID NO:1. The present application also provides the use of the gene as described above in breeding soybeans with increased yield or changed nodule number, optionally, the changed nodule number is manifested as increased nodule number, nodule number per root and / or nodule number per unit root length, optionally, the changed nodule number is manifested as decreased nodule number, nodule number per root and / or nodule number per unit root length. The present application has great theoretical and application value for regulating soybean root nodule symbiotic nitrogen fixation or nodule number and its related application research.

The invention belongs to the technical field of biology, and relates to novel application of protein. The invention discloses an NPE1 protein and an application of a biological material for coding the NPE1 protein in regulating and controlling the catalytic efficiency of a metabolic enzyme. The overexpression of the new gene NPE1 of the soybean in the soybean can obviously enhance the catalytic efficiency of key metabolic enzymes GmPGK3a, GmPFK3a, GmGS1gamma1 and GmPGD2a in root nodules, so that the nitrogen fixation capacity of the soybean root nodules is enhanced, and the biomass and the final yield of overground parts of plants are further improved; the research of the inventor provides gene resources for nitrogen efficiency and molecular breeding of leguminous plants including soybeans, and provides a theoretical basis for molecular design of efficient nitrogen fixation soybean varieties, so that the development of environment-friendly green sustainable agriculture is promoted.

The invention discloses sinorhizobium meliloti MCSWL1 and application thereof, and relates to the technical field of agriculture, the preservation number of the sinorhizobium meliloti MCSWL1 is CCTCC NO: M20231120, the 16S rDNA sequence is shown as SEQ ID NO: 1, and the sinorhizobium meliloti MCSWL1 is obtained by separating and screening from the rhizosphere of sinorhizobium meliloti; the fertilizer is applied to promoting alfalfa growth; the alfalfa is grassland No.3; the sinorhizobium meliloti MCSWL1 provided by the invention can absorb nitrogen in the air and convert the nitrogen into ammonia which can be utilized by plants, so that root nodules are formed, and nitrogen nutrition is supplied to the plants.

The invention belongs to the technical field of agricultural biology, and provides a soybean nodulation and nitrogen fixation related gene and application of an encoding protein thereof, the nucleotide sequence of the gene Gm02g072800 is shown as SEQ ID NO: 1, or a variant sequence which encodes the same functional protein and has homology greater than or equal to 90%; the amino acid sequence of the protein coded by the gene Gm02g072800 comprises a conservative AP2 structural domain, belongs to an ERF transcription factor family, is positioned in a cell nucleus, and has the functions of promoting soybean root nodule development and symbiotic nitrogen fixation. According to the invention, the specific expression of the gene in the soybean middle column and root nodule endodermis is determined, and a target gene is provided for cultivating soybean varieties with high nitrogen fixation efficiency.

The present invention belongs to the field of biotechnology, relates to new applications of genes, and particularly refers to proteins, genes, their editing vectors and applications for controlling the symbiotic nitrogen fixation efficiency of soybeans. The present application has discovered two genes of soybeans GmNAS1 , GmNAP1 and one transcription factor GmNFYC10a , and obtained the gene sequences of the three through reverse genetics means, and successfully isolated, cloned and verified them by using the soybean hairy root transient transformation system, stable transgenic plants, PCR reactions and gene sequencing. The soybean genes GmNAS1 , GmNAP1 and the transcription factor GmNFYC10a control the symbiotic nitrogen fixation efficiency of soybeans through their interactions with each other, revealing the GmNAS1 , GmNAP1 and GmNFYC10a molecular mechanism for improving the nitrogen fixation efficiency of root nodules by regulating the flow direction of PEP.

This invention discloses a genetically engineered rhizobium strain overexpressing purine nucleoside phosphorylase, its construction method, and its applications, belonging to the fields of agricultural biotechnology and genetic engineering. This invention uses *Rhizobium fischeri* (Fragariae spp.) as the basis for its development. Sinorhizobium fredii CCBAU 45436 was used as the starting strain. The strain was introduced by introducing a sample containing the strain itself. rpoD Strong promoter sequences of genes, encoding purine nucleoside phosphorylases deoD The recombinant strain CCBAU 45436 deoD OE was obtained by using a recombinant plasmid composed of the gene sequence and the gene encoding a 3×Flag tag sequence. This recombinant strain has the accession number CGMCC No. 38578 at the China General Microbiological Culture Collection Center (CGMCC). This invention experimentally demonstrates that inoculation... deoD deoD deoD deoD deoD deoD deoD deoD Soybeans overexpressing the recombinant strain (CGMCC No. 38578) showed significantly better plant height and root nodule fresh weight than soybeans inoculated with unmodified wild-type rhizobium strains.

The invention discloses application of a GmNSE1 gene in regulation and control of soybean root nodule nitrogen fixation efficiency. The application of the GmNSE1 gene or a biological material related to the GmNSE1 gene in at least one of the following aspects: A1) regulating and controlling the nitrogen fixation efficiency of soybean root nodules or preparing a product for regulating and controlling the nitrogen fixation efficiency of the soybean root nodules; a2) cultivating soybeans with improved nitrogen fixation efficiency or preparing products for improving the nitrogen fixation efficiency of the soybeans; a3) preparing transgenic soybeans; the nucleotide sequence of the GmNSE1 gene is as shown in SEQ ID NO. 1. The specificity of the GmNSE1 gene is expressed in a root nodule infection area, the GmNSE1 gene has E3 ubiquitin ligase enzyme activity in vitro, and compared with a control group, the nitrogenase activity of a soybean plant with the overexpressed GmNSE1 gene is remarkably improved, which shows that the GmNSE1 gene plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules, and the GmNSE1 gene has a good application prospect in the field of soybean root nodule symbiotic nitrogen fixation. The method has application prospects in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

The invention relates to the technical field of gene engineering, and discloses an application of a Glyma. 11G085500 or Glyma. 01G159200 gene in regulation and control of soybean nodulation. The gene Glyma. 11G085500 and the gene Glyma. 01G159200 are a group of homologous genes in the soybean, and the gene Glyma. 11G085500 and the gene Glyma. It is found for the first time that the group of homologous genes can regulate and control the number of soybean root nodules, new targets and methods are provided for soybean breeding and construction of related research models, and important scientific research and practical significance is achieved.

The present invention relates to a gene GmHB21 involved in soybean nodule vascular bundle development and its use. Specifically, the present invention relates to an isolated gene involved in soybean nodule vascular bundle development or regulating the number of soybean nodules, wherein the coding region sequence of the gene is shown in SEQ ID NO: 1 or a homologous sequence thereof. The present invention also provides a method for regulating the number of plant nodules, comprising introducing the gene described above, or a sequence that inhibits the expression of the gene described above, into target plant cells or tissues to obtain a transgenic plant, wherein the number of nodules in the transgenic plant is altered compared to the target plant, and the plant is a legume, preferably soybean. In addition, the present invention also provides the use of the gene described above, or the sequence that inhibits the expression of the gene described above, in cultivating legumes, such as soybeans, with altered nodule numbers.

The application belongs to the field of bioengineering and particularly relates to a Bradyrhizobium elkanii with improved nodulation capacity and a construction method thereof. The pip gene of the Bradyrhizobium elkanii FG-486 is knocked out to improve the nodulation capacity of the Bradyrhizobium elkanii FG-486, and the Bradyrhizobium elkanii FG-486 delta P with improved nodulation capacity is obtained. Compared with the wild strain FG-486, the average nodulation number of soybean plants after seed dressing with the Bradyrhizobium elkanii FG-486 delta P is increased by 31.15%, the root nodule dry weight is increased by 10.26%, and the soybean yield is increased by 7.85%.

The invention discloses a planting method for sowing astragalus sinicus synchronously with rice fertilization, and belongs to the technical field of agricultural cultivation. Aiming at the defects of low emergence rate and tedious independent operation of the existing astragalus sinicus seeding, the invention provides the following steps: preparing a granular fertilizer to be applied and astragalus sinicus seeds, and carrying out physical dry mixing in an anhydrous state before application to form a uniform seed-fertilizer mixture; before the rice growth period is in the ear differentiation II period, the rice field is adjusted to keep a shallow water layer state or a wet state; and uniformly spreading the seed-fertilizer mixture into the rice field at one time along the rice planting row. According to the method, the habitat conditions in the middle and early stage of rice growth are utilized, granular fertilizer is used as a physical dispersion carrier of seeds, rice fertilization and astragalus sinicus seeding are combined into one operation, the germination problem caused by soil drought in traditional late seeding is solved, the growth period is prolonged, root nodulation and nitrogen fixation are promoted, and the yield is increased. The cultivation effects of saving labor and cost and being light and simplified are achieved.

The invention discloses application of a GmNSE1 gene in regulation and control of soybean root nodule nitrogen fixation efficiency. The application of the GmNSE1 gene or a biological material related to the GmNSE1 gene in at least one of the following aspects: A1) regulating and controlling the nitrogen fixation efficiency of soybean root nodules or preparing a product for regulating and controlling the nitrogen fixation efficiency of the soybean root nodules; a2) cultivating soybeans with improved nitrogen fixation efficiency or preparing products for improving the nitrogen fixation efficiency of the soybeans; a3) preparing transgenic soybeans; the nucleotide sequence of the GmNSE1 gene is as shown in SEQ ID NO. 1. The specificity of the GmNSE1 gene is expressed in a root nodule infection area, the GmNSE1 gene has E3 ubiquitin ligase enzyme activity in vitro, and the activity of a mutant with the GmNSE1 gene knocked out is remarkably reduced compared with that of wild nitrogenase, so that the GmNSE1 gene plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules; the method has application prospects in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

This application provides the use of the MsGRF1c gene in regulating alfalfa nodule senescence, nitrogen fixation efficiency, and biomass. This application demonstrates that the MsGRF1c gene can delay alfalfa nodule senescence, improve nodule nitrogen fixation efficiency, and thereby increase plant biomass under low-nitrogen conditions. This new discovery complements the regulatory mechanism for nodule senescence in legumes and has important utility in reducing nitrogen fertilizer application.

The invention provides application of hydroxypropyl chitosan oligosaccharide in preparation of a plant growth regulator, and belongs to the technical field of plant growth. The hydroxypropyl chitosan oligosaccharide is used as a raw material to prepare the plant growth regulator, new application of the hydroxypropyl chitosan oligosaccharide is provided, the seedling height of plants can be effectively increased, the root length can be effectively increased, the number and / or weight of root nodules can be effectively increased, the number of the root nodules of the plants and / or the activity of nitrogenase can be effectively increased, and the yield of the plants is increased. According to the present invention, the activity of glutathione reductase (GR) and / or glutathione transferase (GST) is improved so as to increase the reduced glutathione content, enhance the oxidation resistance of plants and / or enhance the salt resistance of crops, and after the compound fertilizer is compounded with the calcium-magnesium element fertilizer, the use amount of the compound fertilizer is reduced so as to provide effects of application reducing and effect increasing. The method for preparing the plant growth regulator is simple and easy to implement, environmentally friendly and convenient to popularize and apply.

The invention provides a method for promoting nodulation and growth of alfalfa, which relates to the field of biological breeding, and comprises the following steps: selection and preservation of test materials, selection of breeding, preparation of culture solution, seed treatment, seed germination test, seed germination test, data analysis, and single factor variance statistical analysis by SPSS23.0 to analyze the fresh and dry weight, length, root index, etc. of seeds, seedling growth index, chlorophyll content determination, relative conductivity, and physiological index of seeds. The invention uses alfalfa "Ju Neng 551" seeds as test materials to explore the effects of different concentrations (0.6 g / L ‑1 , 0.9gL ‑1 , 1.2gL ‑1 , 1.5gL ‑1 )The effects of combined inoculation of Cp2‑EPS and rhizobia (R) on seed germination and seedling growth of alfalfa were studied. It was found that combined inoculation of 0.6-1.5 g / L Cp2‑EPS and rhizobia could effectively promote the growth and root nodulation of alfalfa seedlings. Further comprehensive analysis by Topsis showed that combined inoculation of 1.2 g / L Cp2‑EPS and rhizobia had the best effect on promoting the growth of alfalfa seedlings.

The invention relates to the technical field of biology, in particular to a sweet clover MaWRKY76 gene and application thereof.The sweet clover MaWRKY76 gene is cloned firstly, and the expression mode of the sweet clover MaWRKY76 gene under the salt stress condition is analyzed through real-time quantitative PCR; by constructing a yeast heterologous expression vector, the MaWRKY76 is proved to be capable of remarkably improving the salt tolerance of yeast cells. A plant overexpression and RNAi vector is further constructed, sweet clover hairy roots are transformed, and the result shows that the MaWRKY76 not only participates in regulating the symbiotic process of root nodules, but also plays a key role in resisting salt stress. The genetic strategy based on MaWRKY76 can effectively improve symbiotic nitrogen fixation efficiency, plant sustainability and yield of sweet clover under salt stress, provides a theoretical basis for efficient nodulation and nitrogen fixation of leguminous plants under adversity stress, and has positive significance in promoting green sustainable agricultural development.

The invention relates to the technical field of biology, in particular to a sweet clover gene MaGRAS51 and application thereof.The MaGRAS51 gene is cloned, a recombinant expression vector is constructed to verify the function of the MaGRAS51 gene, and it is found that overexpression of the MaGRAS51 gene can promote root nodule symbiosis of sweet clover roots, the salt stress resistance function is achieved, and in a high-salt environment, the salt stress resistance of the sweet clover is improved. The root nodulation of the sweet clover can still be induced. The gene and the discovery of the function show that the genetic strategy taking the MaGRAS51 as the key gene can improve the symbiotic nitrogen fixation efficiency, sustainability and yield of sweet clover under salt stress, a theoretical basis is provided for efficient nodulation and nitrogen fixation of leguminous plants under abiotic environment stress, and the development of green sustainable agriculture is promoted.