46 results about "Plant nodule" patented technology

The invention belongs to the technical field of plant genetic engineering and soybean breeding, and discloses application of a GmCDPKb gene in regulation and control of the number of soybean symbiotic nodules, the nucleotide sequence of the GmCDPKb gene is shown as SEQ ID NO.1, and the amino acid sequence of encoded protein is shown as SEQ ID NO.2. According to the invention, a GmCDPKb gene mutation and overexpression soybean stable genetic transformation plant is constructed, and character investigation shows that the nodulation number of a mutant plant is obviously lower than that of a wild type, and the nodulation number of an overexpression plant is obviously higher than that of the wild type. The result shows that the GmCDPKb gene plays a role in regulating and controlling the number of soybean nodules. The invention reveals that the GmCDPKb gene is a key regulatory factor for regulating the number of soybean root nodules symbiotic nodules, the number of soybean nodules can be stably and obviously regulated by regulating the expression quantity of the gene, and important gene resources and germplasm materials are provided for analyzing a soybean symbiotic nitrogen fixation molecular mechanism and cultivating a new efficient nitrogen fixation soybean variety.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention relates to the technical field of biology, and discloses application of PHR2 in regulating nodulation and nitrogen fixation processes of leguminous plants under phosphorus starvation stress, and the application is any one of the following applications: P1, application in enhancing nitrogen fixation capability of the leguminous plants under the phosphorus starvation stress; p2, application in promoting formation of root nodules with nitrogen fixation capacity of leguminous plants under phosphorus starvation stress; p3, application of improving activity of nitrogenase in leguminous plant root nodules under phosphorus starvation stress; p4, application in leguminous plant breeding. The PHR2 protein and related biological materials thereof can be used for regulating and controlling the nitrogen fixation capacity of leguminous plants under phosphorus starvation stress.

The invention belongs to the technical field of agricultural biology, and provides a soybean nodulation and nitrogen fixation related gene and application of an encoding protein thereof, the nucleotide sequence of the gene Gm02g072800 is shown as SEQ ID NO: 1, or a variant sequence which encodes the same functional protein and has homology greater than or equal to 90%; the amino acid sequence of the protein coded by the gene Gm02g072800 comprises a conservative AP2 structural domain, belongs to an ERF transcription factor family, is positioned in a cell nucleus, and has the functions of promoting soybean root nodule development and symbiotic nitrogen fixation. According to the invention, the specific expression of the gene in the soybean middle column and root nodule endodermis is determined, and a target gene is provided for cultivating soybean varieties with high nitrogen fixation efficiency.

The invention discloses application of a GmNSE1 gene in regulation and control of soybean root nodule nitrogen fixation efficiency. The application of the GmNSE1 gene or a biological material related to the GmNSE1 gene in at least one of the following aspects: A1) regulating and controlling the nitrogen fixation efficiency of soybean root nodules or preparing a product for regulating and controlling the nitrogen fixation efficiency of the soybean root nodules; a2) cultivating soybeans with improved nitrogen fixation efficiency or preparing products for improving the nitrogen fixation efficiency of the soybeans; a3) preparing transgenic soybeans; the nucleotide sequence of the GmNSE1 gene is as shown in SEQ ID NO. 1. The specificity of the GmNSE1 gene is expressed in a root nodule infection area, the GmNSE1 gene has E3 ubiquitin ligase enzyme activity in vitro, and compared with a control group, the nitrogenase activity of a soybean plant with the overexpressed GmNSE1 gene is remarkably improved, which shows that the GmNSE1 gene plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules, and the GmNSE1 gene has a good application prospect in the field of soybean root nodule symbiotic nitrogen fixation. The method has application prospects in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

The invention relates to the technical field of gene engineering, and discloses an application of a Glyma. 11G085500 or Glyma. 01G159200 gene in regulation and control of soybean nodulation. The gene Glyma. 11G085500 and the gene Glyma. 01G159200 are a group of homologous genes in the soybean, and the gene Glyma. 11G085500 and the gene Glyma. It is found for the first time that the group of homologous genes can regulate and control the number of soybean root nodules, new targets and methods are provided for soybean breeding and construction of related research models, and important scientific research and practical significance is achieved.

The application belongs to the field of bioengineering and particularly relates to a Bradyrhizobium elkanii with improved nodulation capacity and a construction method thereof. The pip gene of the Bradyrhizobium elkanii FG-486 is knocked out to improve the nodulation capacity of the Bradyrhizobium elkanii FG-486, and the Bradyrhizobium elkanii FG-486 delta P with improved nodulation capacity is obtained. Compared with the wild strain FG-486, the average nodulation number of soybean plants after seed dressing with the Bradyrhizobium elkanii FG-486 delta P is increased by 31.15%, the root nodule dry weight is increased by 10.26%, and the soybean yield is increased by 7.85%.

The invention discloses a planting method for sowing astragalus sinicus synchronously with rice fertilization, and belongs to the technical field of agricultural cultivation. Aiming at the defects of low emergence rate and tedious independent operation of the existing astragalus sinicus seeding, the invention provides the following steps: preparing a granular fertilizer to be applied and astragalus sinicus seeds, and carrying out physical dry mixing in an anhydrous state before application to form a uniform seed-fertilizer mixture; before the rice growth period is in the ear differentiation II period, the rice field is adjusted to keep a shallow water layer state or a wet state; and uniformly spreading the seed-fertilizer mixture into the rice field at one time along the rice planting row. According to the method, the habitat conditions in the middle and early stage of rice growth are utilized, granular fertilizer is used as a physical dispersion carrier of seeds, rice fertilization and astragalus sinicus seeding are combined into one operation, the germination problem caused by soil drought in traditional late seeding is solved, the growth period is prolonged, root nodulation and nitrogen fixation are promoted, and the yield is increased. The cultivation effects of saving labor and cost and being light and simplified are achieved.

The invention discloses application of a GmNSE1 gene in regulation and control of soybean root nodule nitrogen fixation efficiency. The application of the GmNSE1 gene or a biological material related to the GmNSE1 gene in at least one of the following aspects: A1) regulating and controlling the nitrogen fixation efficiency of soybean root nodules or preparing a product for regulating and controlling the nitrogen fixation efficiency of the soybean root nodules; a2) cultivating soybeans with improved nitrogen fixation efficiency or preparing products for improving the nitrogen fixation efficiency of the soybeans; a3) preparing transgenic soybeans; the nucleotide sequence of the GmNSE1 gene is as shown in SEQ ID NO. 1. The specificity of the GmNSE1 gene is expressed in a root nodule infection area, the GmNSE1 gene has E3 ubiquitin ligase enzyme activity in vitro, and the activity of a mutant with the GmNSE1 gene knocked out is remarkably reduced compared with that of wild nitrogenase, so that the GmNSE1 gene plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules; the method has application prospects in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

The invention relates to the technical field of biology, in particular to a sweet clover MaWRKY76 gene and application thereof.The sweet clover MaWRKY76 gene is cloned firstly, and the expression mode of the sweet clover MaWRKY76 gene under the salt stress condition is analyzed through real-time quantitative PCR; by constructing a yeast heterologous expression vector, the MaWRKY76 is proved to be capable of remarkably improving the salt tolerance of yeast cells. A plant overexpression and RNAi vector is further constructed, sweet clover hairy roots are transformed, and the result shows that the MaWRKY76 not only participates in regulating the symbiotic process of root nodules, but also plays a key role in resisting salt stress. The genetic strategy based on MaWRKY76 can effectively improve symbiotic nitrogen fixation efficiency, plant sustainability and yield of sweet clover under salt stress, provides a theoretical basis for efficient nodulation and nitrogen fixation of leguminous plants under adversity stress, and has positive significance in promoting green sustainable agricultural development.

This invention belongs to the field of microbial technology and discloses a synthetic microbial community for enhancing biological nitrogen fixation in karst grasslands, its construction method, and its application. The synthetic microbial community includes... Sinorhizobium americanum , Sionrhizobium sp. as well as Bacillus cereus Wherein: the Sinorhizobium americanum The accession number is GDMCC NO: 67796; Sionrhizobium sp. The accession number is GDMCC NO: 67797; Bacillus cereus The accession number is GDMCC NO: 67798. For leguminous forage grasses that require repeated sowing, the seeds are treated with a synthetic microbial community before being sown in the field; for perennial leguminous forage grasses that have already been planted, the synthetic microbial community is diluted with water and sprayed onto the surface of adjacent leguminous forage grasses. This invention screens out suitable and efficient root nodule endophytic bacteria in karst areas and constructs a synthetic microbial community to enhance the biological nitrogen fixation of leguminous forage grasses. It clarifies the technical details of the practical application of the synthetic microbial community in the field. The application of this technology can significantly improve the biological nitrogen fixation rate of leguminous forage grasses, significantly reduce the amount of nitrogen fertilizer used in forage grasses, and significantly increase forage grass yield.

The invention discloses a method for efficiently screening rhizobium with functions of promoting growth and inhibiting aspergillus flavus and application of the method, the field ecological pre-enrichment effect of a microbial agent with an ARC function is introduced into a rhizobium screening process for the first time, and the abundance of a target flora is directionally increased from the source through the toxin control and bacterium enrichment effect of the rhizobium. The method comprises the following steps: synchronously collecting rhizosphere soil, root system and root nodule samples, and comprehensively separating rhizobium; a high-throughput primary screening system with parallel growth-promoting and antibacterial functions is constructed, and the nitrogen-fixing growth-promoting capability and the antibacterial effect of volatile substances of candidate strains are synchronously evaluated in an early stage through a pot experiment and a double-vessel buckling method. And further performing functional quantification, multi-gene phylogenetic identification and omics mechanism analysis on the positive strain. The method overcomes the defects that a traditional method is single in function and low in efficiency, and an effective way for rapidly, directionally and systematically screening multifunctional rhizobium resources from an optimized rhizosphere ecosystem is provided.

This invention belongs to the field of genetic engineering technology, specifically relating to soybeans. GmPLAT5 Application of genes in promoting root nodule formation in legumes. This invention utilizes the pZP211 overexpression vector to rapidly obtain transgenic plants through Agrobacterium-mediated genetic transformation of soybean, successfully... GmPLAT5 Overexpression was performed, and the number of root nodules in chimeric transgenic plants was counted. The results showed that... GmPLAT5 The gene can positively regulate the number of soybean root nodules with significant effects. This invention is the first to propose Gm. PLAT5 It can positively regulate the nodulation process in soybeans and has proven that soybeans GmPLAT5 Overexpression of the gene under both water treatment and salt stress conditions can significantly increase the number of soybean root nodules, providing important evidence for further analysis of its salt tolerance molecular mechanism. In the future, it can be applied to soybean production and molecular breeding through transgenic and molecular marker methods, which has great application value.

The invention provides rhizobium japonicum GY4 and application thereof, and relates to the technical field of microorganisms. Comprising soybean rhizobium GY4, the classification name of the soybean rhizobium GY4 is Rhizobium sp, the soybean rhizobium GY4 is preserved in Guangdong Microbial Culture Collection Center on July 15, 2025, the preservation number is GDMCC No. 66686, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The strain belongs to the rhizobium, can significantly improve the abundance of the rhizobium, promote the formation and development of root nodules, increase the number of the root nodules, improve the nitrogen fixation capability of the soybeans, and efficiently promote the growth of the soybeans under the strip relay intercropping condition of the soybeans and the corn, and provides a new strain resource for improving the nitrogen fixation efficiency in the soybean production.

The invention relates to an application method of a special microbial agent for improving the nitrogen fixation efficiency of soybean root nodules, and belongs to the field of agricultural microorganism application. A core strategy of staged time sequence application is adopted: firstly, before sowing or during sowing, a first complex microbial inoculant containing proline secreting bacteria and indigenous infectious microbe inhibiting bacteria is applied into soil, and a pre-culture period is performed for 5-15 days; during the period, proline is converted into indole-3-acetic acid (IAA) by a fourth microorganism which synchronously exists or is introduced, so that a growth-promoting microenvironment is established at the rhizosphere, and meanwhile, a third microorganism inhibits harmful infectious microbes; next, bradyrhizobium or sinorhizobium is applied to the seedlings or rhizosphere. According to the time sequence design, an optimized colonization ecological niche with low competition and high chemotactic signals is created for target rhizobium through a synergistic mechanism of firstly improving the environment and then introducing strains. According to the method, the infection success rate of rhizobium can be remarkably increased, the number of root nodules and nitrogenase activity are increased, soybean root development is synchronously promoted, and the soybean symbiotic nitrogen fixation efficiency and yield are improved.

The invention relates to a soybean root nodule endogenous fomesafen degrading bacterium and application thereof. The classification name of the fomesafen degrading bacterium is Mycobacterium sp. W109, and the preservation number of the fomesafen degrading bacterium is CCTCC (China Center for Type Culture Collection) NO: M 20251729. According to the invention, a fomesafen degrading bacterium is separated from soybean root nodules, the bacterium can stably degrade fomesafen, and the variety of the fomesafen degrading bacterium is expanded.

The application discloses a Neorhizobium rhizobium strain isolated and purified from soybean root nodules and application thereof. The strain is a new species of the Neorhizobium genus through physiological and biochemical determination and phylogenetic analysis, and is named as Neorhizobium glycine EC2-8. The strain re-inoculation experiment shows that the rhizobium strain can invade the soybean roots and form effective nodulation, and can promote the growth of soybeans.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a soybean GmNEN5 gene to regulation and control of plant root nodule nitrogen fixation efficiency and delay of root nodule senescence. The soybean GmNEN5 gene is proved to be a positive regulatory factor for regulating the development of the root nodule and regulating the nitrogen fixation efficiency of the root nodule by overexpressing a coding gene of the soybean GmNEN5 protein in the soybean root nodule, and the condition that the root nodule senescence can be delayed and the nitrogen fixation capability of the plant can be improved by overexpressing the GmNEN5 gene in the soybean is found; the method has a good application prospect in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

The invention provides a microorganism for improving grassland and application of the microorganism for improving the grassland, the microorganism for improving the grassland comprises Bradyrhizobium japonicum TG101, and further comprises one or more of Brevibacterium tumefaciens TG15, Phyllobacterium tumefaciens TG47 and Enterobacter ludwigii DP20, and the microorganism for improving the grassland further comprises one or more of Bradyrhizobium japonicum TG101, the Brevibacterium tumefaciens TG15, the Phyllobacterium tumefaciens TG47 and the Enterobacter ludwigii DP20. According to the application, novel application of the brevibacterium friariorum TG15, the leaf bacillus dulcis TG47 and the enterobacter ludwigii DP20 is innovatively found, that is, the three bacteria and the combination thereof can promote nodulation of the bradyrhizobium elwardii TG101; the compound microbial fertilizer disclosed by the invention has the functions of greatly improving the nodulation quantity, root nodule diameter and nitrogen fixation capability of lespedeza daurica in the field, and greatly improving the yield, quality and medicinal components of lespedeza daurica.

The invention discloses bacillus velezensis and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis provided by the invention is bacillus velezensis, the strain number of the bacillus velezensis is 41S2, and the registration number of the bacillus velezensis in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.35218. The bacillus velezensis provided by the invention is a bacillus velezensis strain. The bacillus velezensis 41S2 provided by the invention has a remarkable growth promoting and yield increasing effect under the condition of saline-alkali stress, and the plant height, the biomass, the root nodule forming ability, the pod number of a single plant and the grain yield of soybeans are remarkably improved; the strain 41S2 can also improve the quality of soybean seeds under the saline-alkali stress condition, including the total carbon content and the total nitrogen content of the soybean seeds are increased and reach 1.77 times and 1.80 times of those of a control group respectively, and the soluble protein content and the isoflavone content of the soybean seeds are increased and reach 1.67 times and 1.91 times of those of the control group respectively.

Provided are compositions and methods employing antimicrobial root nodule specific cysteine-rich (NCR) peptides and variants thereof, including folded variants of NCR peptides (NCRPFV), including folded variants of antimicrobial NCR peptides and proteins (NCRPFV) that exhibit advantageous antifungal properties and desirable resistance or sensitivity to protease cleavage, the antimicrobial NCRPFV may be applied directly ex vivo to a plant, human or animal, or may be applied in vivo to a plant, human or animal.

The invention relates to the technical field of gene engineering, and discloses application of a root nodule related gene in regulation and control of the number of soybean root nodules, and the root nodule related gene is a Glyma. 12G050100 gene or a Glyma. 11G125500 gene. The invention finds that the group of homologous genes Glyma. 12G050100 and Glyma. 11G125500 are related to the number of soybean root nodules for the first time, provides a new target for soybean breeding and construction of a soybean nodulation inhibition model, and has important scientific research and practical significance.

The invention discloses arthrobacter for improving acidic white pulp soil and a microbial agent and application of the arthrobacter. The Arthrobacter sp. W36-1 is preserved in CGMCC (China General Microbiological Culture Collection Center), and the preservation number of the Arthrobacter sp. W36-1 is No. 31903. The bacterial strain is separated from white pulp soil, and is characterized by having efficient biological acid reduction (alkali production) capability, and a culture medium with the pH value of 5.0 can be increased to 7.66 within 72 hours. Under the acid stress condition that the pH value is less than or equal to 5.5, the strain can synchronously play multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing IAA and EPS and the like. Pot experiments prove that the microbial inoculum can effectively improve the pH value of acidic white slurry soil, remarkably relieve damage of acid stress to soybeans, promote root growth of the soybeans, increase the number of root nodules and biomass and have a good growth promoting effect on rice germination under acid stress.

The application discloses a gene GmSCAMP3 for regulating soybean root nodule symbiotic nitrogen fixation ability and application thereof. Specifically, the application provides a protein for regulating soybean root nodule symbiotic nitrogen fixation ability, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. The application also provides a method for improving soybean yield or changing soybean root nodule symbiotic nitrogen fixation ability, which comprises the step of introducing a polynucleotide encoding the protein into a soybean cell or tissue to obtain transgenic soybean. It is found that the nitrogenase activity of Gmscamp3 mutant root nodule is only 65.79% of that of wild type root nodule, indicating that the GmSCAMP3 protein plays an important role in regulating the soybean root nodule symbiotic nitrogen fixation ability.

The application belongs to the field of agricultural microorganism technology, and particularly relates to rhizobium promoting plant cell division, salt and alkali tolerance and alkali reduction and application thereof. Neorhizobium alkalisoli The rhizobium has a strain number YRD01 and a preservation number CCTCC NO: M 20251990, and is preserved in the China Typical Culture Collection Center in Luo Jia Mountain, Wuhan, Hubei on September 9, 2025. The strain obtained in the application is derived from sesbania root nodule and obtained after screening and culture. Neorhizobium alkalisoli The rhizobium YRD01 obtained in the application is a new type of rhizobium with high salt and alkali tolerance, active alkali reduction function and high-efficiency growth promotion characteristics.

The invention discloses a method for screening nitrogen-fixing bacteria LHM15 adapted to peanut varieties, and relates to the technical field of agricultural microbiology, and the method comprises the following specific steps: picking peanut root nodule samples without plant diseases and insect pests in a pod bearing period, storing at 4 DEG C, disinfecting and grinding the root nodules, then coating, culturing and purifying bacterial strains, identifying bacterial colonies and genes in multiple dimensions, and screening the nitrogen-fixing bacteria LHM15. In the seedling stage, inoculating the bacterial liquid and performing contrast culture, and finally determining the optimal nitrogen-fixing bacteria LHM15 according to plant indexes; according to the method, specific peanut germplasm root nodules serve as samples, a multi-dimensional identification system is combined, the problems that nitrogen-fixing bacteria screening lacks pertinence and neglects the nitrogen fixation function are solved, the screened LHM15 is efficiently matched with target peanuts, and the nitrogen fixation function and environmental adaptability are achieved; meanwhile, adaptive link parameters are specifically designed, the application bottleneck is broken through, the colonization rate and nitrogen fixation efficiency are improved, growth indexes are optimized, chemical fertilizer dependence is reduced, special microbial resources are provided, and economic and ecological values are both achieved.

The invention discloses a boschniakia rossica symbiotic cultivation method with alnus japonica as hosts, and belongs to the technical field of boschniakia rossica cultivation The problem that boschniakia rossica symbiotic cultivation is low in survival rate and unstable in yield is solved. The method comprises the following steps: selecting a healthy alnus japonica middle-age forest with the tree age of 10-30 years as a host forest; oblique cuts are cut in the lateral roots of the alnus japonica at the positions 20-40 cm away from the trunk of the alnus japonica; mixing the boschniakia rossica seeds subjected to cold stratification treatment and hormone combined treatment with a biological adhesive to prepare seed paste; coating a root nodule symbiotic nitrogen-fixing actinomycete inoculant at a host root notch, filling the seed paste into the host root notch, covering the host root notch with wet humus soil, and compacting; the humidity of the covered wet humus soil is kept to be 15%-25%, low-concentration compound fertilizer and microbial agents are dressed in May, inoculation points are checked in June and September every year, and inoculation is supplemented if the parasitic rate is lower than 80%; after the boschniakia rossica germinates in the spring of the second year, collecting flower stems.

The application discloses a waxberry CRRSP1 gene and application thereof, and belongs to the technical field of genetic engineering. The application provides a CRRSP1 gene which is specifically up-regulated in the root nodule of a waxberry, and the CDS sequence of the CRRSP1 gene is shown as SEQ ID NO. 1. The application also provides application of the waxberry CRRSP1 gene in promoting symbiotic interaction between a nodule plant and symbiotic bacteria, and the application comprises: up-regulating expression of the waxberry CRRSP1 gene in the nodule plant by using a biological technical means. By up-regulating expression of the gene in the nodule plant, symbiotic nodulation of the nodule plant and the symbiotic bacteria can be significantly promoted, and the plant biomass is increased. The application provides an important reference value for research on symbiotic genes.

The application belongs to the field of biotechnology, and particularly relates to application of nano iron atoms in promoting plant growth and nodule fixation of nitrogen, wherein the nano iron atoms significantly improve the plant height, root length, fresh weight of aboveground part, fresh weight of root, dry weight of aboveground part and dry weight of root of alfalfa; the application mode of seed soaking + spraying is more beneficial to the growth of alfalfa; meanwhile, the root activity of alfalfa under the treatment of FeNPs is significantly higher than that of the control; according to the phenotype of root nodule, the total number of root nodules and the number of effective root nodules under the treatment of FeNPs are 2.04 times and 1.42 times higher than those of the control respectively; the data of nitrogen fixation enzyme activity further prove that the nitrogen fixation enzyme activity under the treatment of FeNPs is the highest. The content of urea further shows that FeNPs promote alfalfa to fix nitrogen in the air and produce a large amount of urea in the root nodule, promote the growth of alfalfa, thereby improve the quality and biomass of leguminous forage, and have wide application prospect.

The application relates to the field of biological technology, in particular to application of cyclic adenosine monophosphate in promoting biomass increase of pasture and root nodule, wherein the cyclic adenosine monophosphate significantly improves the fresh weight biomass of the above-ground part, the root part and the single-plant root system root nodule of Medicago truncatula inoculated with rhizobium, and the difference is significant.