36 results about "Nitrogenase" patented technology

According to the high-temperature-resistant nitrogen-fixing bacteria microcapsule fertilizer and the preparation method thereof, aiming at the problems of thallus inactivation and nutrient loss in a high-temperature environment, a sodium alginate-chitosan double-layer embedding technology is combined with magnetic nanoparticle modification, and a stable double-layer structure is formed by optimizing the ratio of an embedding material, so that the high-temperature-resistant nitrogen-fixing bacteria microcapsule fertilizer is prepared. The survival rate and activity of the nitrogen-fixing bacteria at high temperature are remarkably improved, and meanwhile, the functions of intelligent nutrient release and magnetic recovery are achieved. The fertilizer is prepared from composite microcapsules, amino acid, humic acid and other raw materials according to a specific ratio through magnetic field orientation treatment, multi-stage mixing, atomization granulation and magnetic field screening processes, the particle size is controlled to be 0.5-3.35 mm, and the shelf life is prolonged to 12 months. The method has the technical effects that trehalose-glycerol / sorbitol synergistically forms a glassy protective film to maintain the stability of thalli, gamma-aminobutyric acid activates nitrogenase gene expression, magnetic nanoparticles construct a photosynthetic symbiotic system to optimize rhizosphere oxygen supply, and silanized FeO nanoparticles fix heavy metal ions through electrostatic adsorption, so that the heavy metal ions in the thalli are effectively adsorbed, and the heavy metal ions in the thalli are effectively adsorbed. And the humic acid buffers the pH fluctuation of the soil, so that the nitrogen fixation efficiency is finally improved by 30-50%, and the crop yield is increased by 15-25%.

The invention relates to the technical field of biology, and discloses application of PHR2 in regulating nodulation and nitrogen fixation processes of leguminous plants under phosphorus starvation stress, and the application is any one of the following applications: P1, application in enhancing nitrogen fixation capability of the leguminous plants under the phosphorus starvation stress; p2, application in promoting formation of root nodules with nitrogen fixation capacity of leguminous plants under phosphorus starvation stress; p3, application of improving activity of nitrogenase in leguminous plant root nodules under phosphorus starvation stress; p4, application in leguminous plant breeding. The PHR2 protein and related biological materials thereof can be used for regulating and controlling the nitrogen fixation capacity of leguminous plants under phosphorus starvation stress.

The invention relates to an artificial efficient nitrogen fixation system and a preparation method and application thereof. The artificial efficient nitrogen fixation system contains recombinant engineering bacteria constructed by an artificial combination module and nitrogen fixation chassis microorganisms; wherein the artificial combination module sequentially comprises a constitutive promoter element Pc and a coding gene of methyltransferase selected from nitrogen-fixing pseudomonas stutzeri A1501 in the direction from upstream to downstream. According to the recombinant engineering bacteria constructed by introducing the artificial combination module into the nitrogen-fixing chassis microorganism, the nitrogenase activity of the nitrogen-fixing chassis microorganism can be remarkably improved, the nitrogen-fixing capacity of the nitrogen-fixing chassis microorganism is remarkably improved, and the recombinant engineering bacteria have a relatively good application prospect in the aspect of promoting plant growth.

The invention belongs to the technical field of soil improvement, and particularly relates to stenotrophomonas maltophilia C-GD and application thereof in nitrogen fixation. The nitrogenase activity of the stenotrophomonas maltophilia C-GD is 45.90 (ng / g / 24h), and the stenotrophomonas maltophilia C-GD shows relatively good nitrogen fixation activity and can be used for nitrogen fixation of soil. The invention provides a strain of Stenotrophomonas maltophilia C-GD with a nitrogen fixation function, and after the Stenotrophomonas maltophilia C-GD is prepared into a microbial inoculum to be applied, the key nutrient indexes of organic matters, total nitrogen, total phosphorus, available phosphorus, alkali-hydrolyzable nitrogen and the like in soil can be greatly improved.

The invention discloses application of a GmNSE1 gene in regulation and control of soybean root nodule nitrogen fixation efficiency. The application of the GmNSE1 gene or a biological material related to the GmNSE1 gene in at least one of the following aspects: A1) regulating and controlling the nitrogen fixation efficiency of soybean root nodules or preparing a product for regulating and controlling the nitrogen fixation efficiency of the soybean root nodules; a2) cultivating soybeans with improved nitrogen fixation efficiency or preparing products for improving the nitrogen fixation efficiency of the soybeans; a3) preparing transgenic soybeans; the nucleotide sequence of the GmNSE1 gene is as shown in SEQ ID NO. 1. The specificity of the GmNSE1 gene is expressed in a root nodule infection area, the GmNSE1 gene has E3 ubiquitin ligase enzyme activity in vitro, and compared with a control group, the nitrogenase activity of a soybean plant with the overexpressed GmNSE1 gene is remarkably improved, which shows that the GmNSE1 gene plays an important role in the process of regulating and controlling the symbiotic nitrogen fixation capacity of soybean root nodules, and the GmNSE1 gene has a good application prospect in the field of soybean root nodule symbiotic nitrogen fixation. The method has application prospects in research on nitrogen fixation mechanisms of leguminous crops and agricultural production.

The invention discloses ammonium-tolerant nitrogen-fixing paenibacillus and application thereof, and belongs to the technical field of agriculture. The bacterial strain is preserved in Guangdong Microbial Culture Collection Center on September 19, 2025, the preservation number is GDMCC 66998, and the bacterial strain is named Paenibacillus sp. By taxonomy. The ammonium-tolerant nitrogen-fixing paenibacillus from the red soil rice field is obtained through screening, the strain still shows high nitrogenase activity under the condition of 80-100 mM NH4 < + >, and the limitation that traditional nitrogen-fixing bacteria are prone to being inhibited by ammonium is broken through. In addition, the strain can significantly improve the germination rate of rice seeds, promote the root length, plant height and biomass accumulation of rice seedlings, and show a good growth promotion effect. Due to the characteristic of forming spores, the strain can also be prepared into a microbial fertilizer preparation which is used for nitrogen fertilizer replacement and synergism in a rice field ecological system.

The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.

The invention relates to novel paenibacillus with biomacromolecule degradation capacity and nitrogen fixation and phosphorus solubilization effects and application of the novel paenibacillus, the strain is identified as a novel strain of paenibacillus through multi-phase classification, and the novel paenibacillus has the capacity of degrading biomacromolecules such as starch, cellulose, casein, chitin and lecithin. The method can be applied to degradation of related carbohydrates, proteins and lipid substances and preparation of biological enzymes; the bacillus subtilis has the advantages that the bacillus subtilis can be used for preparing the bacillus subtilis, has the phosphorus dissolving capacity of dissolving organic phosphorus and inorganic phosphorus, has nitrogenase and beta-1, 3-glucanase and the activity of generating IAA (Indoleacetic Acid), can play a probiotic role on crops by fixing nitrogen, dissolving phosphorus and inhibiting pathogenic bacteria, can grow in an environment with the salinity not higher than 4.5%, and has certain salt-tolerant activity. Due to multiple functions, the strain can be used for preparing and applying biological nitrogen fertilizers, phosphate fertilizers and crop probiotics, so that the strain plays an important role in crop growth and green agricultural production.

The invention belongs to the technical field of soil nitrogen fixation and environmental protection, and relates to a method for enhancing soil nitrogen fixation by using pure iron powder. A method for enhancing soil nitrogen fixation by using pure iron powder comprises the following steps: mixing rice soil and wheat straw powder to obtain a soil sample; the method comprises the following steps: mixing a soil sample with pure iron powder under a waterflooding condition; pure iron powder is added to regulate and control microbial ecology of rice soil biological nitrogen fixation, the soil iron reduction process is enhanced, the abundance of iron reducing bacteria in soil is improved, the relative contribution of iron reducing bacteria nitrogen fixation is enhanced to a certain extent, the diversity of iron reducing bacteria communities is reduced, the ecological niches of dominant groups are enhanced, and the ecological niches of the dominant groups are increased. Finally, the nitrogenase activity of the soil is enhanced, and the nitrogen fixation capacity of the rice soil is improved.

Disclosed is a method of producing a non-naturally occurring peptide for reducing nitrogen, the method comprising: a) identifying a first group of peptides each having a binding pocket similar to a naturally occurring iron-sulfur cluster cofactor binding pocket of a naturally occurring nitrogenase, wherein each of the first group of peptides is not a nitrogenase or a subunit or domain of a nitrogenase; b) selecting, from the first group of peptides, a second group of peptides each having at least one target amino acid in the binding pocket; c) selecting, from the second group of peptides, a third group of peptides each having at least one target property; and d) modifying the sequence and / or structure of each of the third group of peptides to improve binding of the iron-sulfur cluster cofactor in the binding pocket. Also disclosed is a non-naturally occurring peptide for reducing nitrogen, comprising: at least one modification to a naturally occurring peptide; and a binding pocket for binding an iron-sulfur cluster cofactor; wherein the naturally occurring peptide is not a nitrogenase or a subunit or domain of a nitrogenase; and wherein the at least one modification improves binding of the iron-sulfur cluster cofactor in the binding pocket.

The invention discloses an activating agent capable of being used for improving the nitrogen fixation activity of farmland soil in situ and a preparation method of the activating agent, and relates to the technical field of agricultural chemistry. The activating agent is prepared from the biochar, the sodium molybdate, the goethite and the rutile as raw materials and is used for improving the nitrogen fixation activity of the farmland soil in situ. On the premise of not adding exogenous strains, by activating existing indigenous nitrogen-fixing microbial communities in farmland soil and stimulating high-efficiency expression of related nitrogen-fixing functional genes in a soil environment, in-situ activation of the nitrogen-fixing potential of the soil is achieved, and the nitrogen-fixing performance of the soil is improved. The activator for in-situ improvement of the nitrogen fixation activity of the farmland soil has a synergistic effect on improving the content of ammonium nitrogen in the soil and the activity of nitrogenase, has the outstanding advantages of high in-situ adaptability, precise action targeting, sustained and lasting nutrition release, environment friendliness, low cost and the like, can remarkably reduce the use of chemical fertilizers, and has a good application prospect. And sustainable development of green agriculture is realized.

The application discloses application of Paenibacillus polymyxa in improving yield of pyrroloquinoline quinone (PQQ), and application of a living silk microorganism in improving nitrogen fixation capacity of Paenibacillus polymyxa. The application also discloses a co-cultivation system with high PQQ yield. The application further discloses the living silk microorganism with a preservation number of CCTCC NO: M 20241604. The application further discloses Paenibacillus polymyxa with a preservation number of CCTCC NO: M 20241603. The application further discloses a fermentation liquor prepared by using the co-cultivation system. The application further discloses application of the co-cultivation system and the fermentation liquor. The application can promote yield of PQQ produced by the living silk microorganism and improve nitrogenase activity of Paenibacillus polymyxa simultaneously in a bidirectional way by co-cultivating and fermenting the living silk microorganism and Paenibacillus polymyxa, and the co-cultivation fermentation liquor can improve crop nitrogen fixation, stress resistance, growth and yield.

The invention relates to an application method of a special microbial agent for improving the nitrogen fixation efficiency of soybean root nodules, and belongs to the field of agricultural microorganism application. A core strategy of staged time sequence application is adopted: firstly, before sowing or during sowing, a first complex microbial inoculant containing proline secreting bacteria and indigenous infectious microbe inhibiting bacteria is applied into soil, and a pre-culture period is performed for 5-15 days; during the period, proline is converted into indole-3-acetic acid (IAA) by a fourth microorganism which synchronously exists or is introduced, so that a growth-promoting microenvironment is established at the rhizosphere, and meanwhile, a third microorganism inhibits harmful infectious microbes; next, bradyrhizobium or sinorhizobium is applied to the seedlings or rhizosphere. According to the time sequence design, an optimized colonization ecological niche with low competition and high chemotactic signals is created for target rhizobium through a synergistic mechanism of firstly improving the environment and then introducing strains. According to the method, the infection success rate of rhizobium can be remarkably increased, the number of root nodules and nitrogenase activity are increased, soybean root development is synchronously promoted, and the soybean symbiotic nitrogen fixation efficiency and yield are improved.

The invention discloses a magnesium modified wheat straw biochar-based slow-release rhizobium fertilizer and a preparation method thereof. The preparation method comprises the following steps: constructing hierarchical porous biochar MgWBC with magnesium-based active sites by adopting magnesium acetate impregnation and high-temperature pyrolysis; granular urea is used as a core, chitosan is used for packaging to realize directional loading and surface phase inversion film sealing of an MgWBC layer and rhizobium, and a composite structure with pH response capability is constructed. According to the method disclosed by the invention, the stress resistance and the directional colonization efficiency of the rhizobium are remarkably improved by utilizing the characteristic that the nitrogenase is activated by magnesium and the refuge effect of pores of the biochar. In a soybean-corn intercropping system, soybean nodulation and nitrogen fixation are enhanced and corn nutrition is met while nutrient slow release is realized through a synergistic mechanism of promoting nitrogen by magnesium. Experiments show that the yield of an intercropping system and the total nitrogen and effective magnesium level of soil can be remarkably improved, and the stability of soil aggregates is improved. The method is of great significance in promoting intercropping weight loss and efficiency improvement and cultivated land conservation.

The invention belongs to the technical field of microbial agents, and particularly relates to a charcoal-based nitrogen-fixing bacterium agent as well as a preparation method and application thereof. According to the invention, a biochar-humic acid-sodium alginate embedding mode is adopted, so that a porous protection cabin can be provided to adsorb plant rhizosphere nutrients. Meanwhile, a low-oxygen microcell is created to protect nitrogenase, physical protection is provided for thalli, and external environmental stress is buffered. Wherein humic acid is used as a high-quality organic carbon source and is beneficial to stimulating thalli to rapidly proliferate in embedded particles and rhizosphere, and a large number of active cells can be obtained in a short time. The biochar-based nitrogen-fixing bacterium agent (namely biochar-humic acid-sodium alginate microspheres) prepared by the invention has the advantages of simple and convenient preparation method, stable storage performance and the like, and compared with other biological agents, the biomass of the dried microbial agent reaches 2.2 * 10 < 10 > CFU / g, so that the biochar-based nitrogen-fixing bacterium agent has a good application prospect.

The invention belongs to the technical field of application of microorganisms, and particularly relates to a diazo nutritional type acetobacter gluconicum mutant, a nitrogen-fixing microbial agent, a preparation method of the nitrogen-fixing microbial agent and application of the nitrogen-fixing microbial agent. The mutant strain with high nitrogenase activity and genetic stability is obtained through ARTP mutagenesis screening, the nitrogen fixation effect is remarkably improved, and the nitrogenase activity of the mutant strain on a nitrogen-free culture medium reaches 368.84 nmol.mg <-1 >. H <-1 >. The nitrogen-fixing bacterial strain disclosed by the invention can effectively improve index values such as plant height, root length and plant weight of rice, has a good growth-promoting effect on various crops, and is a broad-spectrum growth-promoting nitrogen-fixing bacterial strain. The nitrogen-fixing bacterial strain can be rapidly cultured in a short time, and is suitable for industrial production. The diazo nutritional glucose acetobacter mutant is a high-enzyme-activity mutant strain obtained through ARTP mutagenesis and acetylene reduction method screening and has good nitrogen fixation capacity, the nitrogenase activity of the diazo nutritional glucose acetobacter mutant is improved by 3.1 times, and the hereditary stability of the diazo nutritional glucose acetobacter mutant is good after multiple times of subculture.

The application discloses a method for producing hydrogen through Clostridium pasteurianum-Rhodobacter sphaeroides coupling fermentation based on nitrogen limitation and application thereof. In the method, activated Clostridium pasteurianum is subjected to dark fermentation for producing hydrogen in a nitrogen-free culture medium and in an argon environment; then, the pH value of the obtained culture solution is adjusted to 7.0-8.0, the activated Rhodobacter sphaeroides is inoculated after selective sterilization, and the light fermentation for producing hydrogen is carried out in the argon environment. In the application, the fermentation solution of Clostridium pasteurianum does not need to be subjected to centrifugal removal of bacterial bodies and the like, and only needs to be simply adjusted in pH value, so that the light fermentation for producing hydrogen of Rhodobacter sphaeroides can be directly carried out. In the application, the nitrogen limitation is used to inhibit excessive proliferation of bacterial bodies, save carbon source flow, induce high expression of nitrogen fixation enzyme, and open a second high-efficiency hydrogen production path in addition to hydrogenase, so that the hydrogen production yield is further improved while the cost of nitrogen source is reduced. The hydrogen production yield of the application reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The application discloses a gene GmSCAMP3 for regulating soybean root nodule symbiotic nitrogen fixation ability and application thereof. Specifically, the application provides a protein for regulating soybean root nodule symbiotic nitrogen fixation ability, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. The application also provides a method for improving soybean yield or changing soybean root nodule symbiotic nitrogen fixation ability, which comprises the step of introducing a polynucleotide encoding the protein into a soybean cell or tissue to obtain transgenic soybean. It is found that the nitrogenase activity of Gmscamp3 mutant root nodule is only 65.79% of that of wild type root nodule, indicating that the GmSCAMP3 protein plays an important role in regulating the soybean root nodule symbiotic nitrogen fixation ability.

The invention discloses streptomyces LZZY-S40 with broad-spectrum bacteriostatic activity and growth promoting effect and application thereof, and belongs to the technical field of microorganisms. The invention provides streptomyces sp. LZZY-S40 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.30320 in order to solve the problems that in the prior art, streptomyces mostly focuses on single function research, the antibacterial spectrum is relatively narrow, and the agricultural production requirement that various diseases coexist is difficult to meet. The bacterial strain has good broad-spectrum bacteriostatic activity on plant pathogenic fungi, and the bacteriostasis rate of the bacterial strain on plant pathogenic bacteria xanthomonas semitransparent reaches 100%; a spore suspension of the strain has a remarkable promoting effect on wheat root length, bud length and wheat fresh weight; in addition, the streptomyces LZZY-S40 strain can also be applied to preparation of microbial agents for producing extracellular protease, auxin, siderophore, nitrogenase and ACC deaminase.

The application belongs to the field of biotechnology, and particularly relates to application of nano iron atoms in promoting plant growth and nodule fixation of nitrogen, wherein the nano iron atoms significantly improve the plant height, root length, fresh weight of aboveground part, fresh weight of root, dry weight of aboveground part and dry weight of root of alfalfa; the application mode of seed soaking + spraying is more beneficial to the growth of alfalfa; meanwhile, the root activity of alfalfa under the treatment of FeNPs is significantly higher than that of the control; according to the phenotype of root nodule, the total number of root nodules and the number of effective root nodules under the treatment of FeNPs are 2.04 times and 1.42 times higher than those of the control respectively; the data of nitrogen fixation enzyme activity further prove that the nitrogen fixation enzyme activity under the treatment of FeNPs is the highest. The content of urea further shows that FeNPs promote alfalfa to fix nitrogen in the air and produce a large amount of urea in the root nodule, promote the growth of alfalfa, thereby improve the quality and biomass of leguminous forage, and have wide application prospect.

Disclosed is a method of producing a non-naturally occurring peptide for reducing nitrogen, the method comprising: a) identifying a first group of peptides each having a binding pocket similar to a naturally occurring iron-sulfur cluster cofactor binding pocket of a naturally occurring nitrogenase, wherein each of the first group of peptides is not a nitrogenase or a subunit or domain of a nitrogenase; b) selecting, from the first group of peptides, a second group of peptides each having at least one target amino acid in the binding pocket; c) selecting, from the second group of peptides, a third group of peptides each having at least one target property; and d) modifying the sequence and / or structure of each of the third group of peptides to improve binding of the iron-sulfur cluster cofactor in the binding pocket.

The application provides application of MoS2@Fe3O4 nanoparticles in promotion of soybean nodule fixation nitrogen additives and a preparation method thereof, and relates to the field of special nanofertilizer for soybean production. The MoS2@Fe3O4 nanoparticles can improve the activity oxygen buffer capacity of soybean root nodules, promote the growth and reproduction of the bacteria in the root nodules, significantly improve the nitrogen fixation enzyme activity of the soybean, promote the accumulation of nitrogen in the root nodules and the leaves, optimize the transport efficiency of nitrogen from the roots to the leaves, and increase the dry matter accumulation of the aboveground part of the soybean. Meanwhile, the nanoparticles can also improve the development state of the soybean root nodules, increase the number and fresh weight of the root nodules. The MoS2@Fe3O4 nanoparticles are synthesized by a self-assembly hydrothermal method, the preparation method is simple and low in cost, the nanoparticles can be applied in the preparation of special nanofertilizer for soybean production, and a new, efficient and stable method is provided for solving the problem of low nitrogen fixation efficiency in soybean production.

The invention provides application of GmSTF1 and GmSTF2 genes in increasing the number of soybean root nodules, nitrogenase activity, biomass and yield, and belongs to the technical field of gene engineering. It is proved for the first time that the GmSTF1 and GmSTF2 genes can regulate and control the soybean root nodule nitrogen fixation capacity, and the number and nitrogen fixation efficiency of soybean root nodules can be remarkably increased by knocking out the GmSTF1 and GmSTF2 genes. The CRISPR / Cas9 gene editing vector constructed by the invention is used for transforming a receptor soybean plant to obtain a transgenic plant, so that the root nodule number, nitrogenase activity and biomass of the soybean can be remarkably improved, and the CRISPR / Cas9 gene editing vector has important significance for improving the yield of the soybean.

MICROBIAL FERTILIZER AS WELL AS PREPARATION METHOD AND USE THEREOF The microbial fertilizer includes a biochar and a-symbiotic N-fixing bacteria inoculated on the biochar carrier, wherein the a-symbiotic N-fixing bacteria are Klebsiella sp. The maize straw-derived biochar with a particle size of 0.25-2 mm has a more significant effect on improving nitrogenase activity. The biochar with a particle size of 0.25-2 mm provides good habitat for strain cells and can preserve moisture and fertility better to ensure the growth and reproduction of strains. The loading effect of the maize straw-derived biochar is superior to that of woody biochar. Relative to the woody biochar, the C: N ratio of straw biochar is lower, which is beneficial for growth of N-fixing bacteria. The preparation of the fertilizer improves total N and microbial biomass N contents in soil, particularly the maize straw-derived biochar has a more significant effect on increasing soil microbial biomass N content, which is closely related to soil microbial activity.

The invention discloses a biological hydrogen fertilizer which comprises the following substances in parts by weight: 4 parts of humic acid, 15 parts of algin, 10 parts of amino acid, 15 parts of chitin, 10 parts of enzyme bacteria, 5 parts of bacillus megatherium, 5 parts of nitrogenase bacteria, 5 parts of nitrogen, 16 parts of phosphorus, 12 parts of potassium and 3 parts of magnesium hydride. The soil conditioner can improve and activate soil, promote root system development and plant growth robustly, can resist drought and waterlogging by using hydrogen molecules, can generate obvious physiological changes after plant cell walls encounter drought and waterlogging stress, contains various beneficial microorganisms, can effectively inhibit pathogenic bacteria in soil, reduces plant diseases, increases the yield, promotes the effect and improves the quality, and has a wide application prospect. Acidolysis and salt decomposition, rotten root and dead plant prevention, sterilization and parasite expelling, flower and fruit promotion, plant robustness, branch increase, empty shell reduction, rich fruit, and yield and income increase are realized.

The invention relates to the technical field of sculellaria barbata culture, in particular to a sculellaria barbata culture medium and a preparation method thereof The sculellaria barbata culture medium is prepared from an MS culture medium and a basic raw material according to the mass ratio of (6-7): 1, and the basic raw material is prepared from the following raw materials in parts by weight: 2-4 parts of novel nano silicon dioxide, 0.05-0.08 part of cerous nitrate, 1-2 parts of an organic fungicide, 0.7-0.8 part of 6-benzyladenine, 0.2-0.4 part of alpha-naphthylacetic acid, 0.1-0.3 part of jasmonic acid methyl ester and 2-4 parts of a functional additive. According to the invention, cell wall stability is enhanced and hormone slow release regulation is realized through novel nano silicon dioxide, and cerium nitrate is improved to activate a flavonoid compound synthetase system; the organic fungicide inhibits pathogenic bacteria and promotes nitrogen conversion by secreting lipopeptide antibiotics and nitrogenase systems; and the functional auxiliary agent realizes efficient retention and stable release of the active components.

The application belongs to the field of microorganisms and particularly relates to a biological nitrogen-fixing bacterium and preparation and application of a microbial inoculum.The biological nitrogen-fixing bacterium is named Microbacterium sp GSS16, classified and named as Microbacterium sp, and preserved in the Guangdong Microbial Culture Collection Center, No. 59 Building, 5th Floor, Guangzhou Xianlie Middle Road 100 Courtyard, on November 14, 2022, with a preservation number of GDMCC No. 62976; and can be used for plant nitrogen fixation, production of nitrogen-fixing enzyme, promotion of plant growth and improvement and fertilization of soil.

The invention relates to the technical field of microorganisms, and discloses a paenibacillus engineering strain PG97 as well as a construction method and application thereof, a point mutation engineering strain PG97 is obtained through site-specific mutagenesis of glnA genes of paenibacillus, a mutant PGlnA-P97L (PG97) is obtained by replacing a 97th proline codon (CCG) of glutamine synthetase encoded by the point mutation engineering strain PG97 with a leucine codon (CTG), and the mutant PGlnA-P97L (PG97) is obtained by the construction method of the paenibacillus engineering strain PG97. The mutant keeps nitrogenase activity under the ammonium concentration of 0-100 mM, and breaks through the inhibition effect of high-concentration ammonium on biological nitrogen fixation; meanwhile, the extracellular ammonium secreting capacity is remarkably improved, more effective nitrogen sources are provided for plants, the growth promoting effect on cucumbers and rice is improved, and wide application prospects are achieved in the field of agricultural production.

The invention provides a nitrogen-fixing paenibacillus sp. SY6, a microbial preparation containing the nitrogen-fixing paenibacillus sp. SY6 and application of the nitrogen-fixing paenibacillus sp. SY6, and belongs to the technical field of agricultural microorganisms and environmental microorganisms. The preservation number of the bacillus SY6 is CGMCC (China General Microbiological Culture Collection Center) No. 35901. The paenibacillus SY6 disclosed by the invention is derived from rhizosphere of Trifolium repens L., and the nitrogenase activity of the paenibacillus SY6 is obviously higher than that of common combined nitrogen-fixing bacteria. Whole genome analysis shows that the paenibacillus SY6 has a complete molybdenum-dependent (nif) and vanadium-dependent (vnf) double nitrogenase system at the same time, and has the capability of synthesizing indoleacetic acid (IAA) through an indole-3-pyruvic acid (IPyA) pathway. The paenibacillus SY6 can be effectively colonized at the rhizosphere of non-host crops such as corn and the like, can promote the elongation of plant root systems, nitrogen absorption and biomass accumulation, and is suitable for farmland nitrogen management, nitrogen fertilizer reduction, soil ecological restoration and microbial fertilizer development.