102 results about "Acinetobacter calcoaceticus" patented technology

The invention relates to acinetobacter calcoaceticus X.L.Zhu.SGD07-3-16 with free living nitrogen fixation capability. The acinetobacter calcoaceticus is preserved in China general microbiological culture collection center, and the preservation number of the acinetobacter calcoaceticus is CGMCCNo.5455. The acinetobacter calcoaceticus is high in nitrogenase activity and phosphorus and potassium dissolving capability, and has ACC (1-aminocyclopropane-1-carboxylic acid) deaminase activity, a certain amount of indoleacetic acid can be generated, and the generation of siderophore is avoided; when an acinetobacter calcoaceticus-containing liquid or solid microbial agent is used for inoculating crops such as corns, oilseed rapes and leaf mustard, the growth of the crops can be remarkably promoted, and the yield of the crops can be increased.

The invention relates to heterotrophic nitrification-aerobic denitrification composite microbial inoculum with salt tolerance and high ammonia nitrogen tolerance, preparation and application thereof.The composite microbial inoculum is prepared by compounding of Acinetobacter calcoaceticus TNJ-1, pseudomonas stutzeri, Halomonas denitrificans and Cupriavidus sp. SWA1. Through the synergistic actionof the four microorganisms, the effective treatment of high ammonia nitrogen wastewater can be realized under aerobic and high-salt conditions, and toxic substances such as chlorides, heavy metals and the like can be effectively resisted.

The invention relates to a composite microbial inoculum for treating micro polluted source water under the low temperature condition and a preparation method thereof, in particular to a composite microbial inoculum and a preparation method thereof, aiming at solving the problems that the existing microbial inoculum has low biological activity and slow growth velocity under the low temperature condition; when organic matter and ammonia nitrogen are treated in the water, organic and toxic substances are difficult to degrade, and nitrite and nitrate are accumulated; and secondary pollution for the environment is caused by the generated N2O. The product composite microbial inoculum is made of acinetobacter lwoffiiSRA10, acinetobacter calcoaceticus PHEA-2, bacillus megatherium X2 and fluid nutrient medium. The method comprises: 1. activation treatment is carried out on raw strain; 2. accumulation culture; 3. after being mixed according to the volume ratio, the strain is added into the fluid nutrient medium; finally, the composite microbial inoculum is obtained. The obtained composite microbial inoculum has high biological activity and fast growth and can be used for treating the micro polluted source water with remarkable effect under the low temperature condition; furthermore, the nitrite and the nitrate are not accumulated, and no N2O is generated, so that the composite microbial inoculum is safe and reliable.

A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome is disclosed. PQQGDH is, for example, a water-soluble PQQGDH derived from Acinetobacter calcoaceticus. The cytochrome is, for example, an electron transfer domain of quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni. The fusion protein of the present invention shows intramolecular electron transfer from PQQ, a redox center, to the cytochrome, which allow construction of a direct electron transfer-type glucose sensor which requires no electron mediators.

The invention relates to the field of treating environment by using microorganisms, in particular to acinetobacter calcoaceticus T32 capable of metabolizing furazolidone, and application thereof. The acinetobacter calcoaceticus T32 has been registered and collected in the China General Microbiological Culture Collection (CGMCC) on sixth, November, 2010; and the collection number is CGMCC No.4311. The acinetobacter calcoaceticus T32 can be used as bactericide for degrading the furazolidone. The furazolidone in the environment is removed by the metabolic process of T32, and the acinetobacter calcoaceticus T32 has the advantages of high degradation speed, low cost, convenience of operation and the like.

A method for repairing soil at high speed and increasing the power of bacteria to emulsify petroleum includes such steps as preparing BH culture medium from MgSO4.7H2O, K2HPO4, KH4PO4, FeCl3 or FeSO4, NH4NO3, CalCl2, citric acid and water, culturing Acinetobacter calcoaceticus for 5 days while rotating at 180 rpm, adding diesel oil and fermented liquid, laying aside, cyclone oscillation, and laying aside.

The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

A modified pyrroloquinoline quinone-dependent glucose dehydrogenase having a low reactivity with respect to maltose, galactose, etc. is provided. A modified pyrroloquinoline quinone-dependent glucose dehydrogenase including an amino acid sequence in which one or more amino acids in a region corresponding to a first region consisting of amino acids at positions 326 to 354 in pyrroloquinoline quinone-dependent glucose dehydrogenase derived from Acinetobacter calcoaceticus are substituted as compared with an amino acid sequence of the corresponding wild-type enzyme.

Disclosed is a modified glucose dehydrogenase having pyrroloquinoline quinone as a coenzyme, wherein one or more amino acid residues in a region of amino acid 349-377 of water-soluble PQQGDH derived from Acinetobacter calcoaceticus is replaced with other amino acid residues and has an inhibition constant (Ksi) of 200 mM or more. The modified water-soluble PQQGDH of the invention can be utilized for measuring glucose levels in the presence of high concentrations of glucose because of the low substrate inhibition by glucose.

The invention provides a rhodobacter sphaeroides strain and a preparation method and application thereof. P-hydroxybenzoic acid transportprotein pcaK gene from acinetobacter calcoaceticus is introduced into the rhodobacter sphaeroides strain, the ability of taking p-hydroxybenzoic acid from an extracellular medium of the rhodobacter sphaeroides strain is enhanced, and therefore, the yield of synthesizing coenzyme Q10 in the rhodobacter sphaeroides strain is improved. By the rhodobacter sphaeroides strain, the shortcomings that in production of coenzyme Q10 by microbiological fermentation, the yield is low, the production cost is high and the like are overcome, the yield of the intracellular coenzyme Q10 of rhodobacter sphaeroides can be improved remarkably, and the cost of industrialized production of the coenzyme Q10 is reduced.

The invention provides a strain of endophytic phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing acinetobacter calcoaceticus and application thereof. The acinetobacter calcoaceticus WYS-A01-1 was deposited in China General Microbiological Culture Collection Center (CGMCC) on July 3, 2017, and the preservation number is: CGMCC No.14378. The endophytic acinetobacter calcoaceticus ofphyllostachys edulis has the functions of solubilizing phosphate, solubilizing potassium and fixing nitrogen, can colonize plants, promote photosynthesis of the phyllostachys edulis, improve biological enzyme activity, and play an important role in regulating plant growth and development.