8 results about "Bacillus firmus" patented technology

The invention relates to the technical field of biological agents, and particularly discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent provided by the invention comprises compound bacillus, a carrier and a coating agent, the composite bacillus comprises bacillus velezensis, bacillus atrophaeus and bacillus firmus with the viable count ratio of (3-7): (1-4): 2; the viable count of the compound microbial agent is greater than or equal to 5 * 10 < 9 > cfu / g; the invention also provides a preparation method of the compound microbial agent. The preparation method comprises the following steps: strain fermentation, carrier adsorption, granulation and enveloping and low-temperature drying. The compound microbial agent provided by the invention can improve the soil structure, enhance the soil fertility and inhibit soil-borne diseases, so that the growth condition of crops is improved, and the quality and yield of the crops are improved.

The invention discloses a toadstool white mould disease biocontrol bacterial preparation and a preparation method thereof, and relates to the technical field of microbial bactericides, the toadstool white mould disease biocontrol bacterial preparation comprises the following raw materials by weight: 37.5 parts of Bacillus firmus fermentation broth, 0.5 part of a nano-material, 45 parts of a carrier, 5 parts of a wetting agent, 10 parts of a dispersant and 2 parts of a protective agent, the nano-material is silica, the carrier is diatomite, the wetting agent is Tween 20, and the dispersant is diatomite. The dispersant is polyvinyl alcohol, and the protective agent is dextrin; according to the toadstool white mould disease biocontrol bacterium preparation and the preparation method thereof, biocontrol bacteria are preferentially colonized at key sites such as plant rhizosphere, leaf surface or wound and the like, so that limited ecological niche is occupied, and effective infection is difficult to establish due to lack of physical space and attachment sites when pathogenic bacteria invade; by adding the nano material, the carrier, the wetting agent, the dispersing agent and the protective agent, the environmental stability and the field persistence of the biocontrol bacterium are improved.

The application discloses a bacillus firmus GXU-Z9 for preventing and treating coral bacterial whitening, the bacillus firmus GXU-Z9 has a nucleotide sequence shown in SEQ ID NO:1, and is used for preventing and treating the whitening of antipathella japonica caused by pathogenic bacteria Vibrio coralliilyticus The bacillus firmus GXU-Z9 is obtained through antagonistic experiment screening, and then is identified through PCR and 16S rRNA gene sequencing. The bacillus firmus GXU-Z9 obtained through screening can antagonize the coral pathogenic bacteria Vibrio coralliilyticus Cause the whitening of coral, is applied to the prevention of specific diseases of coral, has great significance to the protection of coral reef resources and the improvement of the survival rate of artificial coral transplantation, and has certain positive effects on helping China to achieve long-term development of coral reef ecosystem protection.

The invention provides a bacillus firmus Sys-Gs-002 strain for producing ginsenoside Rg1, the strain is preserved in China Center for Type Culture Collection, the preservation date is November 24, 2025, the preservation number is CCTCC NO: M20252684, and the preservation number is CCTCC NO: M20252684. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The bacillus firmus Sys-Gs-002 strain provided by the invention can produce ginsenoside Rg1 through metabolism, can be subjected to large-scale fermentation culture in a short time, is relatively low in fermentation cost, is not limited by conditions such as time domain and season, and has remarkable advantages. Meanwhile, the invention also provides optimized microbial fermentation conditions and a formula of the culture medium, and the yield of the ginsenoside Rg1 is relatively high.

The present invention relates to a fusion protein having a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member and an enzyme having serine protease activity, wherein the enzyme having serine protease activity is from Bacillus firmus or is a variant of such enzyme. The present invention also provides a recombinant Bacillus cereus family member that expresses such fusion protein and exosporium fragments derived from such recombinant Bacillus cereus family member. Methods of using such recombinant Bacillus cereus family members or exosporium fragments derived therefrom for nematode control are also provided.

The invention relates to a preparation method of a carbon-based microbial fertilizer, and belongs to the technical field of agricultural microbial fertilizers. The method comprises the following steps: inoculating a bacillus firmus TB-7 strain into an NA liquid culture medium, and culturing for 24 hours under the condition of a constant-temperature shaking table at 37 DEG C to obtain a strain fermentation solution; sterilizing the biochar for 60 minutes under the condition of 121 DEG C; mixing the fermentation liquor with the sterilized biochar according to the mass ratio of 1: 2, and regulating the water content to 30%, so that the content of effective viable bacteria in the mixture is not less than 3 * 10 CFU / g; and culturing the mixture at a constant temperature of 37 DEG C for 24 hours to obtain the carbon-based microbial fertilizer. The culture medium components of the TB-7 strain are optimized through a single factor experiment and an orthogonal experiment, and the thallus activity and yield of the TB-7 strain are improved. The obtained bacterial fertilizer has the characteristics of high bacterial content and stable preparation conditions, and can be applied as a base fertilizer in the Chinese cabbage planting process. The invention provides the preparation method of the carbon-based microbial fertilizer, which is simple and convenient in preparation process, strong in strain adaptability and high in carrier stability.

The application discloses an environment-friendly biological organic fertilizer for improving acidified soil and a preparation method thereof, and belongs to the technical field of organic fertilizer. The biological organic fertilizer is prepared from the following raw materials in parts by weight: 10-20 parts of composite gel material, 80-100 parts of mature poultry manure, 10-20 parts of microbial agent, 30-50 parts of modified waste tea leaf biochar, 10-15 parts of urea, 5-10 parts of humic acid, 1-5 parts of superphosphoric acid calcium, and 1-5 parts of sodium carboxymethyl cellulose; the microbial agent is prepared by mixing bacillus firmus, pseudomonas plecoglossicida and azotobacter chroococcum at a volume ratio of 1:1:1. The biological organic fertilizer can significantly improve the soil pH, increase the content of organic matter in the soil, has good buffering capacity and long-term effect, and can enhance the water and fertilizer conservation capacity of the soil, and has a remarkable effect on increasing the yield and income of crops.

The invention relates to a mud flat saline-alkali soil microorganism-plant-hydrogen-rich water synergistic improvement method and application in the technical field of environmental engineering. Aiming at the technical problems that an existing strain cannot be applied to improvement of heavy metal polluted saline-alkali soil and lacks a compound functional strain, the invention provides a ternary synergistic improvement method. Bacillus firmus L71 and mulberry enterobacter H41 are mixed according to the mass ratio of 1: 1 to prepare a complex microbial inoculant, the spore number is larger than or equal to 1 * 10 < 8 > / g, and the viable count is larger than or equal to 5 * 10 < 8 > cfu / mL; 50-100 kg of the microbial inoculum is applied per mu according to the saline-alkali degree of the soil in a grading manner, and the microbial inoculum is mixed with the straw crushed to 5-10 cm according to the proportion of 20% Hydrogen-rich water with the dissolved hydrogen concentration of 0.7-1.0 mg / L is adopted for irrigation treatment, and irrigation is conducted according to needs; and planting 10-15 suaeda salsa, suaeda salsa or pennisetum sinese salt-tolerant plants per square meter. According to the method, the total salt removal rate of the soil reaches 30% or above, the organic matter content is increased by 1.5-10%, the plant growth promoting rate reaches 40% or above, the heavy metal bioavailability is reduced by 35% or above, and efficient comprehensive treatment of the heavy metal-salt and alkali combined contaminated soil is achieved.