82results about How to "High synthesis efficiency" patented technology

The invention discloses an ROS-responsive prostate cancer targeting prodrug as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The prodrug is formed by covalently linking a prostate cancer targeting peptide SYEELR, a ROS (reactive oxygen species) response type ketal thiol (TK) connexon and an active molecule Devimistat. The preparation method comprises the following two steps: firstly, coupling Devimistat with a TK linker to obtain an intermediate Dev-TK, and then coupling the intermediate Dev-TK with an SYEELR peptide fragment to obtain a target product. The prodrug can specifically break and release Devimistat in a tumor high active oxygen microenvironment, and active targeting delivery is realized by virtue of the SYEELR peptide. The invention provides a novel prostatic cancer treatment strategy with accurate targeting, controllable release, enhanced curative effect and toxicity reduction potential.

The invention discloses a catechol side group-containing polylysine derivative as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving catechol acid in an organic phase, firstly adding NHS, stirring and dissolving until the materials are uniformly mixed, then adding EDC.HCl, and stirring and reacting to obtain a catechol-NHS intermediate solution; (2) dissolving polylysine or a salt thereof in a water phase, and adjusting the pH value to 6.0-7.0 by using an alkaline solution to obtain a solution of polylysine or a salt thereof; and (3) adding the intermediate solution obtained in the step (1) into the polylysine or the salt solution thereof obtained in the step (2), and maintaining the pH value of the reaction system at 5.0-6.0 by using an alkaline solution to obtain the catechol side group-containing polylysine derivative. The preparation method is short in reaction time and mild in reaction condition, and the obtained product is high in grafting rate and high in raw material conversion rate and has a good application prospect in the aspects of tissue adhesives and the like.

The invention relates to the field of biological catalysis, and discloses a method and a device for generating ursodesoxycholic acid through continuous flow catalysis of chenodeoxycholic acid. Comprising the following steps: (1) in the presence of first immobilized cells, carrying out a first catalytic reaction on a reaction system containing chenodeoxycholic acid to obtain a first product, the first immobilized cells expressing 7 alpha-hydroxysteroid dehydrogenase and lactic dehydrogenase; the concentration of the chenodeoxycholic acid in the reaction system is 40 to 70 mM; (2) adjusting the pH value of the first product to 8-9, and then in the presence of a second immobilized cell, carrying out a second catalytic reaction on the first product after the pH value is adjusted to obtain ursodesoxycholic acid, and the second immobilized cell expresses 7 beta-hydroxysteroid dehydrogenase and glucose dehydrogenase. According to the method, the stable activity of a catalytic system is kept, efficient conversion of high-concentration CDCA is achieved, meanwhile, precipitation of 7-KLCA is thoroughly avoided, and the synthesis efficiency, the product purity and the process continuity of UDCA are remarkably improved.

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

The invention discloses a method for rapidly and efficiently preparing a series of novel ester group facaplysin derivatives by combining EDCI and HOBT to react with carboxyl facaplysin and directly carrying out ester exchange reaction on an active ester intermediate and alcohol without separation. The invention also discloses application of the derivatives in resisting methicillin-resistant staphylococcus aureus and escherichia coli. The preparation method comprises the following steps that EDCI and HOBT are combined to activate carboxyl facaplysin for reaction, an activated ester intermediate is generated, the activated ester intermediate and alcohol are directly subjected to esterification reaction without separation, and a series of ester-based facaplysin derivatives are obtained. According to the preparation method, ester-based Fascaplysin derivatives with different structures can be rapidly and efficiently constructed, and compared with a traditional method that ester-based carboline derivatives are firstly synthesized and then high-temperature intramolecular cyclization is performed, the time and the raw material cost are greatly saved, the synthesis efficiency is improved, the reaction process is observable and controllable, and the yield is stable. And the synthesized ester group facaplysin derivative has excellent antibacterial activity on methicillin-resistant staphylococcus aureus and escherichia coli.

The application relates to the technical field of biotechnology, in particular to a dopamine reuptake inhibitor intermediate synthesis enzyme mutant and application, and amino acid sequences are shown in SEQ ID NO: 7-SEQ ID NO: 12. The stereoselectivity of asymmetric reduction catalysis of the dopamine reuptake inhibitor intermediate precursor is improved, the R-type dopamine reuptake inhibitor intermediate with high chirality purity is obtained, and the synthesis efficiency of the dopamine reuptake inhibitor intermediate is improved.

The invention belongs to the field of medicinal chemistry, and particularly relates to a colopivir synthesis process which is simple in process route, cheap and safe in reagent, free of heating and cooling operation, low in energy consumption, good in product quality, high in yield and wide in commercial development prospect, the synthesis efficiency and operation convenience are greatly improved, and the time cost is reduced.

The invention relates to a method for improving PD-1 titer produced by Chinese hamster ovary cells. The method comprises the following steps: inoculating the Chinese hamster ovary cells into an initial culture medium for culturing; after the culture is performed for 48 hours, feeding a fed-batch culture medium every day, and maintaining the glucose concentration in the culture medium to be 1.3-1.7 g / L, the galactose concentration to be 0.6-1.0 g / L and the L-sodium lactate concentration to be 14-16 mM; the culture temperature is reduced, a fed-batch culture medium is fed every day, the glucose concentration in the culture medium is maintained at (2-2.8) g / L, the galactose concentration is maintained at (1.7-2.2) g / L, and cells are harvested. Galactose is introduced to serve as a second carbon source, and control over the culture initial metabolism environment and the carbon source in the culture stage is combined, so that the maximum accumulation amount of lactic acid is obviously reduced, the cell culture period is remarkably prolonged, and the maximum titer of the PD-1 antibody is remarkably improved compared with an existing process.

The invention discloses a carbon nitride composite material as well as a preparation method and application thereof, and designs and prepares an activated carbon loaded modified carbon nitride nanosheet, K ions are doped between layers of the modified carbon nitride nanosheet to form local geometric distortion caused by KN bridging and K + intercalation, and the local geometric distortion and a P gap state generate synergy, so that the performance of the carbon nitride composite material is improved. The adsorption and activation capabilities of the material on O2 molecules are jointly optimized, so that the reaction from * OO to * OOH is obviously accelerated. The adsorption and enrichment effects of the activated carbon and the oxidative degradation and sterilization capabilities of the photocatalytic material can be fully utilized.

The invention provides a glycosyl transferase gene for catalyzing astragalus smicus glycoside synthesis and application of the glycosyl transferase gene, and belongs to the technical field of biology. The invention develops a novel glycosyl transferase, a novel glycosyl transferase mutant is obtained by utilizing a genetic engineering method, and astragalus smicus glycoside can be synthesized through a microbial fermentation or in-vitro enzyme reaction process. The synthetic route is simpler, the synthetic condition is mild, the environmental pollution is small, the synthetic efficiency is higher, the purity is better, the waste of environmental resources is less, a new synthetic route is provided for the synthesis of the chemical substance, namely, the astragalin with high economic value, and a new thought and method are provided for actual production.

The application provides a method for synthesizing N,N-diethyl-2-hydroxyphenylacetamide and analogues thereof, and a reaction route of the method is as follows: the method comprises the following steps: 1) reacting a compound of formula (III) with a compound of formula (IV) in a solvent A under the action of a condensing agent and a base for a certain time to obtain a reaction solution containing a compound of formula (II); 2) adding a reducing agent to the reaction solution containing the compound of formula (II) obtained in step 1), and reacting for a certain time; the reaction is monitored, and after the reaction is completed, the solvent is spun dry, separated and dried to obtain a crude product of the compound of formula (I); 3) purifying the crude product obtained in step 2) to obtain a pure product of the compound of formula (I); wherein R1 and R2 are independently hydrogen, an alkyl group or an aryl group; and A is a substituted or unsubstituted aryl group or a heteroaryl group.

The present application relates to the technical field of auxiliary structure of cubic press, in particular to a temperature control device of cubic press, which is applied to cubic press, and the cubic press comprises six top hammers, each of which is provided with small and large pads, and a steel ring is detachably sleeved on the top hammer, the small and large pads and the top hammer; the temperature control device of cubic press comprises a cooling liquid pipe, each top hammer is provided with a cooling liquid pipe, both ends of the cooling liquid pipe pass through the small pad, and the cooling liquid pipe is detachably arranged on the steel ring, the cooling liquid pipe is configured to receive external cold liquid, thereby breaking through the heat transfer bottleneck of traditional indirect cooling of steel ring, so that the heat of the top hammer can be directly radiated through the cooling liquid pipe, while significantly reducing the interface thermal resistance and material thermal conductivity loss in the heat conduction path, the temperature stability of the superhard material synthesis process is ensured, and the synthesis efficiency and product quality are improved.

The invention relates to the technical field of biology, in particular to a biological preparation method of a remegapam intermediate, which comprises the following steps: by taking remegapam intermediate precursor ketone with a certain concentration as a substrate, adding recombinant engineering bacteria, reacting in a reaction system with the pH value of 8.0-9.0 at the temperature of 30-40 DEG C, and obtaining a product after the reaction is completed, wherein the recombinant engineering bacterium is an engineering bacterium containing a ketoreductase mutant coding gene, and the amino acid sequence of the ketoreductase mutant is as shown in SEQ ID NO: 4, SEQ ID NO: 19 or SEQ ID NO: 39. According to the biological preparation method of the remegapam intermediate disclosed by the embodiment of the invention, the stereoselectivity of asymmetric reduction catalysis of the used ketoreductase mutant on precursor ketone of the remegapam intermediate is improved, the R-type remegapam intermediate with high chiral purity is obtained, and the synthesis efficiency of the remegapam intermediate is favorably improved.

The application discloses a phosphorescent on-off type biological orthogonal iridium complex probe and a preparation and application thereof, and belongs to the technical field of organic photoelectric materials. A benz[h]quinoline-5,6-dione derivative is used as a C^N ligand, the C^N ligand is subjected to a coordination reaction with an iridium trichloride trihydrate to obtain a cyclometalated iridium chloro-bridged dimer, and then the cyclometalated iridium chloro-bridged dimer is subjected to a reaction with an N^N ligand to obtain the iridium complex probe. After the probe is reacted with a biological orthogonal unit, angelica lactone, the excited state energy level of the complex is changed, the photophysical property of the complex is influenced, the phosphorescence is turned on from nothing, and the effect of phosphorescence on-off is achieved. The phosphorescence on-off characteristic makes the probe before and after the reaction with the angelica lactone present obvious luminescent signal ratios. The characteristic can maximally reduce background signal interference, is convenient for cell imaging and biomolecular recognition, and can provide a new train of thought for developing a novel phosphorescent on-off type biological orthogonal material. The application has a good application prospect in the field of biological marking and imaging.

The present invention relates to a recombinant halomonas comprising an exogenously introduced xylose metabolic pathway and a metabolic pathway for synthesizing a copolymer of hydroxybutyric acid (HB) and lactic acid (LA), whereby the copolymer can be synthesized from a xylose-containing cellulose hydrolysate, the LA ratio can be controlled by further promoter modification and process optimization, and industrial enlargement is facilitated.

This invention discloses a miniaturized four-channel combining module based on the TE20 mode, belonging to the field of millimeter-wave power combining technology. It includes a layered upper cavity, a mezzanine, a lower cavity, and an RF unit. The RF unit comprises a TE10-TE20 waveguide distributor, two amplification branches, and a TE20-TE10 waveguide combiner. Each amplification branch includes a TE20 mode filter, a waveguide-microstrip transition, a pair of amplifier chips, and a microstrip-waveguide transition connected sequentially. The TE10-TE20 waveguide distributor and the TE20 mode filter are disposed in the upper and lower cavities. The waveguide-microstrip transition, the pair of amplifier chips, and the microstrip-waveguide transition are disposed on opposite sides of the mezzanine. The TE20-TE10 waveguide combiner is disposed in the upper cavity, the mezzanine, and the lower cavity. This invention utilizes a high-isolation waveguide distributor / combiner to improve branch isolation, shorten the combining path, and has a compact overall structure with advantages such as wide bandwidth, high isolation, high combining efficiency, and miniaturization.

This invention discloses Ni2Fe(SO4) 0.5 (OH)6(H2O) 3.85 This paper discusses / NF catalysts, their preparation methods, and applications, belonging to the field of catalyst synthesis technology. Using large-size nickel foam as a substrate, Ni₂Fe(SO₄) is directly prepared through one-step in-situ microwave-driven growth. 0.5 (OH)6(H2O) 3.85 The nanosheet array eliminates the need for subsequent loading or additional processing, simplifying the process and enhancing the bond between the catalyst and the substrate. This catalyst exhibits excellent performance in hydrogen production via water electrolysis, particularly in seawater electrolysis.

The invention discloses a synthesis method of a trifluoromethanesulfonate radical promoted amidino nickel complex. The preparation method comprises the following steps: reacting N, N '-dialkyl substituted amidino alkali metal salt with a nickel precursor, adding trifluoromethanesulfonate as an accelerant, and regulating the reaction rate and selectivity by utilizing the coordination effect of trifluoromethanesulfonate so as to efficiently prepare the amidino nickel complex. In the process, an ether compound is used as a solvent, the reaction is carried out at 0-70 DEG C for 1-48 hours, the reaction yield can reach 90% at most, and the product purity is gt; and 98%. The method has the characteristics of easily available raw materials, simplicity and convenience in operation, mild reaction conditions, good target product selectivity, high synthesis efficiency, convenience in post-treatment and the like, and has industrial application prospects.

The application relates to the technical field of reaction kettles, and discloses a medicine synthesis reaction kettle which comprises a reaction kettle body, a temperature control assembly, a stirring assembly and a control assembly. The medicine synthesis reaction kettle is characterized in that the reaction kettle body and the temperature control assembly are cooperatively designed, a cooling jacket and a cooling spiral pipe form a double-channel cooling system, the cooling efficiency is improved, the required low-temperature condition can be quickly reached, the flexible switching of heating and cooling during the reaction is realized by combining the regional layout of the electric heating wire, the temperature regulation and control requirement of complex reactions is met, under the regulation and control of the control assembly, the temperature sensor monitors data in real time and feeds back to the controller, the work of the first circulating pump and the second circulating pump is dynamically adjusted, the temperature change can be more accurately controlled, the selectivity of the reaction and the product quality are improved, and the servo motor in the stirring assembly drives the stirring blade to strengthen the heat transfer uniformity, so that local overheating or uneven cooling is avoided.

The application discloses a kind of for the synthesis of bio-based polyether polyol impact jet stirring reactor, it is related to reactor technical field, including reactor body, adjusting mechanism, charge air mechanism and stirring mechanism, the left end of the top of the reactor body is fixedly connected with the outer wall of adjusting mechanism, the left end of the reactor body is fixedly connected with charge air mechanism, the top center of the reactor body is fixedly connected with stirring mechanism, adjusting mechanism is by adjusting four groups of nozzle and shape change, change jet position and intersection, optimize flow field adaptation multiple raw materials;Guide mechanism adjusts the angle of guide vane and cooperates nozzle, double control flow field prevents uneven mixing;Charge air mechanism controls wave-shaped air bag by vacuum pump and piston rod, optimizes flow field to avoid scale formation;Stirring mechanism relies on part rotation and jet coordination, adaptation scale and promote gas-liquid mass transfer efficiency, finally improve synthesis efficiency, product uniformity and product quality.

The application belongs to the field of oligonucleotide synthesis, and particularly relates to a styrene / nitrogen-containing heterocyclic copolymer and a glass carrier and a preparation method and application thereof. The modified styrene / nitrogen-containing heterocyclic copolymer has a structure shown in formula (2'). The key of the application is that the nitrogen-containing heterocyclic compound and the alkyl ether fragment are introduced into the polystyrene molecular chain, which can effectively improve the effective load of nucleotides, and compared with the nitrogen-containing heterocyclic compound introduced into the oligonucleotide synthesis system alone and the styrene / nitrogen-containing heterocyclic copolymer system without the alkyl ether fragment, the synthesis efficiency and purity of the oligonucleotide can be significantly improved, and the application is particularly suitable for long-chain oligonucleotide synthesis. Formula (2')

The application discloses a fluorine-modified chitosan derivative used as a drug carrier, which has the following structure: a fluorine-containing compound is covalently connected to a chitosan main chain, the molecular weight of the chitosan ranges from 5000 to 5000000, and the fluorine-containing compound is a fluorine-containing aliphatic chain shown in the following formula (I) or an aromatic ring functional group shown in the following formula (II), wherein R1 is halogen (fluorine, chlorine, bromine or iodine), halogen-substituted alkane, cycloalkane, aldehyde group, carboxyl group, double bond, acetylene bond, hydroxyl group, sulfonyl chloride, sulfonic acid bond or sulfydryl, which are active groups capable of reacting with primary amino groups. The application aims to provide a novel drug carrier material with obvious drug absorption promotion effect and low toxicity, and the synthesis process of the fluorine-containing compound-modified chitosan is mature, simple to operate, high in synthesis efficiency, short in period, and capable of obtaining a high-yield drug carrier without complicated purification steps, so that the simple synthesis method provides a good foundation for commercialization.

This invention discloses a method for preparing a PdCu / CP electrode and its application, as well as an electrocatalytic hydrogenation-aldehyde oxidation battery. The preparation involves using a mixed aqueous solution of palladium and copper sources as the electrodeposition solution, and carbon paper as the working electrode. Electrochemical deposition is performed in a three-electrode system using a chronoamperometry method. The deposition potential is -0.6 to -0.1 V vs. Ag / AgCl, and the deposition time is 10-30 min. Pd and Cu are loaded onto the carbon paper in an alloy form to obtain the PdCu / CP electrode. The molar ratio of Pd in ​​the palladium source to Cu in the copper source in the electrodeposition solution is 1:1.2-1.5. The PdCu / CP electrode prepared by this invention can simultaneously serve as both cathode and anode for electrocatalytic hydrogenation and aldehyde oxidation. Furthermore, it can efficiently generate high-value chemicals simultaneously at both the anode and cathode without external power, effectively reducing energy consumption. The high synthesis efficiency of high-value-added chemicals demonstrates significant application potential.

This invention discloses a one-pot method for preparing amidine-lanthanum complexes. Specifically, the lanthanum salt first reacts with an ether compound; the product, without separation, is then directly reacted with an N,N'-dialkyl-substituted amidine alkali metal salt to prepare the amidine-lanthanum complex in a one-pot process. The reaction yield can reach up to 90%, and the product purity is >98%. This invention features readily available raw materials, simple operation, mild reaction conditions, good selectivity for the target product, high synthesis efficiency, and convenient post-processing, making it promising for industrial applications.

The application discloses MCM-22 molecular sieve, a preparation method and application thereof. The application adopts a small amount of N,N,N-trimethyladamantylammonium and cyclohexylamine dual organic structure directing agents in a hydrothermal synthesis system, and synthesizes MCM-22 molecular sieve through one-step crystallization and calcination. The method can directly synthesize MCM-22 molecular sieve without adding seeds and without temperature change crystallization operation, and the yield of the MCM-22 molecular sieve prepared after calcination is high. The content of non-framework aluminum in the MCM-22 molecular sieve prepared by the preparation method is less than 5% of the total aluminum content, the content of aluminum located at the half-supercage T2 site of the framework is higher than 10% of the total aluminum content, and the MCM-22 molecular sieve has good performance when used as a catalyst for a liquid phase alkylation reaction of benzene and ethylene to prepare alkylbenzene.

The invention discloses a preparation method of an azo reactive dye, which comprises the following steps: reacting cyanuric chloride with sodium 2, 5-diaminobenzene sulfonate to obtain a first condensate, diazotizing the first condensate, performing acidic coupling on the first condensate and acetyl J acid, performing alkaline coupling on the first condensate and a diazo solution of 6-beta-ethyl sulfuryl sulfate-2-naphthylamine-1-sulfonic acid, and finally performing secondary condensation on the second condensate and nicotinic acid to obtain the azo reactive dye. Reacting to obtain azo reactive dye primary pulp, wherein the structure of the dye is shown in the specification; the prepared dye is bright in color, excellent in solubility, high in storage stability, good in lifting power and good in fixation rate and color fastness.

The application provides a cell-free synthesis system, a kit and a preparation method thereof for synthesizing a GLP-1 related single / dual / triple target receptor agonist polypeptide or fusion protein. By using a cell-free protein expression system, GLP-1 related variants or fusion proteins and the like can be successfully expressed, and the protein yield and biological activity are high, the production process of the GLP-1 related polypeptide or protein is shortened, and the cost is reduced.

The utility model discloses a biological reagent medicament supplement container device relates to biological reagent supplement technical field, the utility model discloses a storage container is connected with the timing controller who has fixedly connected outside the wall of storage container, the top outer wall fixed connection of storage container has the feeding pipe, the outer wall fixed of storage container has the sucking disc seat, the utility model discloses a stirring blade is set up, starts first motor, and first motor will drive the rotation of worm, and worm will drive the rotation of worm wheel, and worm wheel will drive the rotation of special sleeve rod, and special sleeve rod will drive the rotation of stirring blade and helical gear, and helical gear will drive the rotation of helical gear no.

This invention belongs to the field of new energy and hydrogen energy, and specifically relates to an integrated energy vessel, the "Dongfang Heping," for direct seawater hydrogen production, storage, and conversion. The integrated energy vessel of this invention includes a powered platform, on which are installed a wave energy system, a photovoltaic system, a wind power system, a seawater desalination direct electrolysis hydrogen production system, a green methanol synthesis system, a green ammonia synthesis system, and a power system. The wave energy system, photovoltaic system, and wind power system are used to extract energy from the ocean and convert it into electrical energy to provide power to the powered platform, the seawater desalination direct electrolysis hydrogen production system, the green methanol synthesis system, and the green ammonia synthesis system. The power system drives the powered platform, and the seawater desalination direct electrolysis hydrogen production system, the green methanol synthesis system, and the green ammonia synthesis system are electrically connected to the power system. This invention provides a comprehensive energy ship system that integrates multiple renewable energy sources such as photovoltaic, wind power, and wave energy. It constructs a power platform at sea that integrates the production, storage, transportation, and utilization of "oceanic green hydrogen," forming an integrated energy production and conversion model of "green electricity-green hydrogen-green ammonia and alcohol." This not only meets the shipping industry's demand for green fuels but also effectively solves the problem of renewable energy consumption, promoting the deep integration of transportation and energy.

The invention relates to a Moorella thermoaceticus strain as well as a preparation method and application thereof, the invention provides the Moorella thermoaceticus strain for synthesizing n-butyl alcohol, the Moorella thermoaceticus strain is Moorelh thermoaceticus GTLB-iB2312 and is preserved in the China General Microbiological Culture Collection Center on September 19, 2024, and the preservation number of the Moorelh thermoaceticus strain is CGMCC No: 46146. The invention further provides a preparation method and application of the Moorelh thermoaceticus strain for synthesizing n-butyl alcohol. Through high expression of the thl gene and simultaneous knockout of silencing pta and ptb genes, the yield of n-butyl alcohol is increased. Through the editing, the concentration and the product conversion rate of the n-butyl alcohol produced by using the synthesis gas, especially hydrogen, as the raw material are greatly increased, more efficient production is realized, and the synthesis efficiency is improved.