334results about How to "High synthesis efficiency" patented technology

The present application relates to the field of biotechnology, and in particular to an epimerase for synthesizing D-tagatose and its application, wherein the epimerase produces a mutation at position 100, or at position 125, or at position 127, or at position 131, or at position 268, or at position 270, or at position 271, or at position 308, or at position 339, or at position 340, or at position 342, or at position 366, or at position 402, or at position 437, or at position 440 in the amino acid sequence shown in SEQ ID NO. 142; through the above-mentioned method, the epimerase exhibits excellent epimerization activity at the 4th carbon position of D-fructose, can efficiently convert D-fructose into D-tagatose, and is beneficial to improving the synthesis efficiency of D-tagatose.

The invention discloses a glycosyl transferase mutant for synthesizing rebaudioside M and a synthesis method of rebaudioside M, and belongs to the technical field of gene engineering. The glycosyl transferase mutant for synthesizing the rebaudioside M is a glycosyl transferase UGT76G4 mutant and / or a glycosyl transferase UGT91C1 mutant, and the glycosyl transferase mutant is a glycosyl transferase UGT76G4 mutant. The glycosyl transferase UGT76G4 mutant is obtained by mutation of an amino acid sequence as shown in SEQ ID NO: 1: 85th amino acid is mutated into R from L, and 90th amino acid is mutated into G from I; the glycosyltransferase UGT91C1 mutant is obtained by mutating an amino acid sequence shown in SEQ ID NO: 3 as follows: the 204th amino acid is mutated into A from L, the 208th amino acid is mutated into A from F, and the 380th amino acid is mutated into D from G. The mutant can realize efficient catalytic synthesis of rebaudioside M, and the yield of rebaudioside M synthesized by an enzymic method is improved.

The invention belongs to the technical field of biochemical engineering and the technical field of bioengineering, and discloses a schizochytrium limacinum engineering strain for producing EPA (eicosapentaenoic acid) by prolonging a desaturation path. Part or all of the extended desaturase genes from thraustochytrium sp. And fusarium sp. And saprolegnia are expressed. According to the invention, extended desaturase genes from various sources are integrated into a schizochytrium limacinum genome, so that a schizochytrium limacinum gene engineering strain efficiently expresses the extended desaturase genes, and a heterologous EPA synthesis path with high synthesis efficiency, short culture period, simple purification method and low cost is provided; a basis is provided for producing grease rich in EPA from schizochytrium limacinum, and the problems of non-sustainability, high cost and the like caused by obtaining EPA from fishes are solved.

The invention relates to the technical field of microalgae biological intelligent control, in particular to a microalgae growth prediction and optimization method and system based on machine learning. Based on the characteristic variable random forest model of the microalgae growth parameters, screening out a characteristic variable data set after dimensionality reduction screening which significantly affects the microalgae growth, and performing regression training of potential principal component variables on the characteristic variable data set through a machine learning algorithm to obtain a trained microalgae growth prediction model; acquiring a plurality of actual prediction results under dynamic change of culture conditions by using the trained microalgae growth prediction model, and searching a given parameter regulation space in a genetic algorithm based on the actual prediction results of microalgae growth culture to obtain an optimal culture condition parameter set; and issuing the optimal culture condition parameter set to an actual culture process of microalgae growth. According to the method, microalgae growth dynamics can be accurately described in a multi-dimensional parameter space, and real-time prediction and optimization of the microalgae growth trend and metabolic yield are realized.

The invention relates to the technical field of solid-state batteries, in particular to a solid-state electrolyte composite membrane, a preparation method thereof and a solid-state battery. The preparation method comprises the following steps: performing ultrasonic-initiated cross-linking polymerization on a polymer solid electrolyte monomer, a cross-linking agent and a surface hydroxylated oxide solid electrolyte to obtain a polymer-oxide solid electrolyte matrix, washing the polymer-oxide solid electrolyte matrix, and soaking the polymer-oxide solid electrolyte matrix in an electrolyte to obtain the solid electrolyte composite membrane. According to the invention, crosslinking of the polymer electrolyte monomer is initiated by ultrasound, and chemical bonds are formed between part of the polymer electrolyte monomer and the surface-hydroxylated oxide solid electrolyte, so that the interfacial compatibility of the polymer electrolyte monomer and the surface-hydroxylated oxide solid electrolyte is effectively improved, and the ionic conductivity, chemical stability and mechanical strength of the polymer electrolyte are improved; due to the properties of easy processing, good flexibility and the like of the polymer electrolyte, the interface compatibility between the oxide solid electrolyte and the positive and negative pole pieces can be fully improved, the flexibility and the processability are improved, and a win-win effect is achieved.

The invention relates to a culture method for improving duckweed protein, which comprises the following steps: purifying duckweed in a Hoagland working solution to obtain purified duckweed; mixing the biogas slurry with the Hoagland working solution to prepare a duckweed culture solution; wherein the concentration of the biogas slurry is 1.5%-5%, and the concentration of the Hoagland working solution is 95%-98.5%; after the purified duckweed is put into the duckweed culture solution, the duckweed culture solution is put into an incubator with preset culture conditions to be cultured, and the cultured duckweed is obtained. By adding the biogas slurry into the Hoagland working solution, the protein content of the cultured duckweed can be remarkably increased, the biogas slurry can be efficiently utilized, water resources are saved, an ideal way is provided for wastewater recycling, meanwhile, nitrogen and phosphorus in the biogas slurry can be utilized for promoting synthesis of duckweed protein, the protein synthesis efficiency is improved, and the yield of duckweed is increased. And a good basis is provided for feed research of duckweed.

The invention discloses a carbon dioxide adsorbent and a preparation method and application thereof.The preparation method comprises the following steps that a biological shell material is cleaned and then dried, the pretreated biological shell material is ground, and coarse shell powder is obtained; sequentially calcining and grinding the coarse shell powder in a protective atmosphere to obtain shell micro powder; mixing the shell micro powder with a magnesium oxide solid phase, calcining in a protective atmosphere, and grinding to obtain the carbon dioxide adsorbent. According to the invention, a solid-phase mixing method is adopted, and the marine biological waste with specific content and magnesium oxide are mixed, so that the mutual cooperation and mutual synergism among the raw material components can be fully exerted, the element composition and microstructure of the adsorbent material can be effectively regulated and controlled, and by virtue of the preparation method which is easy to operate and low in cost, the adsorbent material can be prepared. The obtained novel carbon dioxide adsorbent can improve the CO2 adsorption capacity and cycle stability of MgO, and can stably maintain high carbon dioxide adsorption rate and adsorption capacity for a long time.

The invention provides a method for efficiently synthesizing basic nickel carbonate, and belongs to the technical field of chemical preparation. Comprising the following steps: (1) preparing a mixed solution of sodium carbonate and sodium hydroxide; (2) under low-temperature and high-speed stirring conditions, dropwise adding a nickel salt solution into the mixed solution, adding a competitive adsorbent, adjusting the pH value of the solution, then heating, continuing stirring reaction, separating the competitive adsorbent by a magnet, centrifuging, washing and collecting prepared primary basic nickel carbonate; and (3) recrystallizing the primary basic nickel carbonate in an ethanol water solution, and drying to obtain the basic nickel carbonate. According to the preparation method disclosed by the invention, the content of impurity ions in the product is reduced, so that the high-purity basic nickel carbonate can be prepared, the synthesis efficiency is high, the production cost is relatively low, the method is green and environment-friendly, the competitive adsorbent can also be repeatedly utilized and recycled, the production cost is reduced, and the method has a wide application prospect.

The invention provides a method for producing pullulan by aureobasidium pullulans, which is characterized in that a microbial conversion method is adopted for the first time, and pullulan is produced by catalyzing conversion of fructooligosaccharide through an enzyme reaction liquid, and the pullulan is produced by taking an aureobasidium pullulans seed culture solution as a carrier, fructooligosaccharide as a substrate and the enzyme reaction liquid as a catalyst. In a 5.0 L biological reaction container, a mode of one-time substrate addition or multiple feeding of fructooligosaccharide and enzyme reaction liquid is adopted, catalytic reaction is performed for 60-72 hr, 61.43 g / L-71.43 g / L of fructooligosaccharide is consumed together, the highest yield of pullulan is 60.2 g / L-64.26 g / L, and the conversion rate of fructooligosaccharide is up to 87.5%-97.98%. Compared with the existing method for producing the pullulan by a fermentation method, the biotransformation method can solve the problem of insufficient enzyme amount of catalytic reaction depended in the pullulan synthesis process on one hand, and also can solve the problem of reverse regulation of the substrate and the product on pullulan synthesis in the pullulan synthesis process on the other hand; and the problems of low substrate carbon source utilization rate, low substrate conversion rate and the like caused thereby are solved.

The invention relates to the technical field of biology, in particular to a leucine dehydrogenase mutant and application thereof. According to the leucine dehydrogenase mutant and the application thereof provided by the embodiment of the invention, wild leucine dehydrogenase is subjected to mutation screening to obtain the leucine dehydrogenase mutant with an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2, the catalytic efficiency of asymmetric reductive amination of a prochiral alpha-keto acid compound is improved, and the thermal stability of the leucine dehydrogenase mutant is improved; the synthesis efficiency of the L-amino acid is favorably improved.

The invention belongs to the technical field of biology, and discloses a method and application for regulating and controlling the fermentation process of schizochytrium limacinum through plant hormones to improve the oil titer, and the method is characterized in that in the fermentation process of schizochytrium limacinum, one or more plant hormones are added into a culture medium to promote accumulation of oil. Plant hormones are added into a fermentation culture medium of schizochytrium limacinum, the plant hormones comprise gibberellin (GB), 6-benayl aminopurine (6-BAP), forchlorfenuron (CPPU) and brassinolide (BR), and the plant hormones promote grease accumulation by adjusting activity of enzymes related to fatty acid synthesis, so that the plant hormones can adjust metabolic pathways of the schizochytrium limacinum and promote grease accumulation. The synthesis efficiency and the yield of the grease are obviously improved.

The invention relates to the technical field of biochar preparation materials, and discloses a preparation method of a modified coal gangue-municipal sludge-based biochar soil conditioner, which comprises the following steps: crushing a coal gangue raw material and a municipal sludge raw material to obtain coal gangue powder and municipal sludge powder; the coal gangue powder and the municipal sludge powder are leached to obtain leached coal gangue powder and leached municipal sludge powder, the leached coal gangue powder and the leached municipal sludge powder are mixed to obtain mixed solid waste powder, the mixed solid waste powder is pyrolyzed to obtain pyrolyzed biochar, the pyrolyzed biochar is acidized to obtain acidized biochar, and the acidized biochar is acidized to obtain acidized biochar. The acidified biochar is subjected to ball milling, and first modified biochar is obtained; active sites of the first modified charcoal are increased, and the target charcoal soil conditioner is obtained. The soil improvement effect of the biochar can be improved.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing L-arginine as well as a construction method and application of the genetically engineered bacterium. Escherichia coli is taken as a starting strain, and the following treatment is performed: overexpressing genes gdhA and gdh of glutamate dehydrogenase, promoting generation of glutamic acid, overexpressing and coding arginine transporter gene argO to enhance extracellular transport of arginine, introducing exogenous argJ to replace a linear synthesis pathway in large intestines, improving the utilization rate of acetyl coenzyme A and accelerating the synthesis efficiency of arginine; a glutamine synthetase gene glnA is over-expressed, and glutamine synthesis is enhanced; the pckA is overexpressed to promote ATP synthesis; meanwhile, speA and adiA genes for coding the arginine decarboxylase are deleted, so that further synthesis of putrescine from L-arginine is inhibited, and the L-arginine accumulation capability of the strain is improved. Through the combined action of the treatment, a genetically engineered bacterium with extremely high L-arginine production yield is finally obtained, and the shake flask fermentation yield can reach 13.7 g / L.

The invention discloses a group of mitochondrial signal peptides and application thereof, and a group of signal peptides with a mitochondrial targeting function is obtained for the first time by screening endogenous mitochondrial key functional proteins of rhodotorula. The invention further realizes expression of a target gene through mitochondrial signal peptide mediation, realizes expression of a target product synthetic pathway gene in rhodotorula mitochondria, and successfully constructs synthetic pathways of terpene such as limonene, linalool, patchouli alcohol and kaurene, branched chain alcohol such as isobutanol and organic acid such as 3-hydracrylic acid. Further, carbon metabolic flow of pyruvic acid flowing to the target product is dredged, and synthesis of the mitochondrial region indoor target product is finally realized. The characterization of a group of rhodotorula mitochondrial signal peptides disclosed by the invention can realize the synthesis of mitochondrial regional chamber target products, and lays a foundation for the research on the realization of metabolic engineering transformation in subcellular organelles.

The invention discloses a PEG lipid compound and application thereof. The structure of the PEG lipid compound is shown as a formula (I), and x is a positive integer selected from 11-17. The PEG lipid compound provided by the invention has good biocompatibility and excellent in-vivo mRNA delivery efficiency. The PEG lipid compound disclosed by the invention is high in synthesis efficiency and low in cost, and is convenient for large-scale production and commercial application. According to the present invention, the delivery effect of the PEG lipid compound corresponding to the LNP formula exceeds the delivery effect of the commercialized ionizable lipid SM-102 and the commercialized PEG lipid DMG-PEG2000; # imgabs0 #

Engineering bacteria for expressing an alpha-glucose phosphate mutase gene, a glucose phosphate mutase gene, a difunctional enzyme glucose phosphate isomerase / mannose 6-phosphate isomerase gene and a mannose 6-phosphate phosphatase gene are used as a whole-cell catalyst, starch or a starch derivative is used as a substrate, phosphate and Mg < 2 + > are added, and the whole-cell engineering bacteria are obtained. A whole-cell catalysis system is constructed, and starch or starch derivatives can be efficiently catalyzed to synthesize D-mannose. According to the method, the D-mannose is prepared through whole-cell catalysis, and the method has the advantages of being low in cost, low in pollution, high in yield and the like and is suitable for industrial production of the D-mannose.

The invention provides a method for constructing planar chiral [2, 2] paracyclophane by cobalt-catalyzed asymmetric carbon-hydrogen bond activation-kinetic resolution, which comprises the following steps: by using a cobalt catalyst, a chiral ligand and an additive, taking a graphite felt electrode as an anode and taking a platinum sheet electrode as a cathode, carrying out racemization on [2, 2] paracyclophane under the condition of direct-current electrolysis, so as to obtain the planar chiral paracyclophane. The preparation method comprises the following steps: carrying out asymmetric carbon-hydrogen bond dehydrogenation coupling / kinetic resolution on 2, 2] paracyclophane formamide and aryl acid, and after the reaction is finished, carrying out corresponding post-treatment to obtain planar chiral [2, 2] paracyclophane formamide and planar chiral oxopara-[2, 2] paracyclophane; according to the method, electrochemical synthesis of planar chiral oxara-[2, 2] paracyclophane is realized through a method for catalyzing asymmetric carbon-hydrogen bond activation by virtue of a crustal high-yield transition metal, a reaction substrate is wide in universality and excellent in stereoselectivity, and a product and a split and recycled raw material can have an excellent ee value (up to gt; 99% ee) and a splitting factor (up to 1057).

The invention discloses a method for preparing CaLa2S4 infrared ceramic powder with higher purity and better crystallinity, which comprises the following steps: synthesizing a precursor in a wet chemical path, carrying out thermal decomposition reaction under a relatively low-temperature condition, and carrying out low-temperature vulcanization to form a CaLa2S4 phase, thereby preparing the CaLa2S4 infrared ceramic powder with higher purity and better crystallinity. According to the method, a CaLa2S4 precursor is chemically synthesized through a liquid phase reaction, thioacetamide serves as a catalyst and an end-capping reagent, impurity elements in the precursor are removed through a thermal decomposition reaction, then the stoichiometric ratio of sulfur is recovered through a low-temperature vulcanization method, and the submicron CaLa2S4 powder is obtained through vulcanization. The method is simple in preparation process, high in synthesis efficiency and low in raw material cost, and the prepared CaLa2S4 powder has the advantages of being high in purity, good in crystallinity and sinterability, small in particle size and the like.

The invention discloses an MMLV reverse transcriptase mutant, which belongs to the technical field of biology and is obtained by mutation design of specific sites of wild type MMLV reverse transcriptase. The mutation site comprises one or a combination of more of A32, S56, E69, L139, T163, T197, H204, S232, N249, T306, F309, T330, G424, L435, D449, D524, E562, D583, L603, H642 and T664, and the mutation site comprises one or a combination of more of the A32, the S56, the E69, the L139, the T163, the T197, the H204, the S232, the N249, the T306, the F309, the T330, the G424, the L435, the D449, the Compared with the existing MMLV reverse transcriptase, the MMLV reverse transcription mutant disclosed by the invention has higher reverse transcription temperature and rate. The invention also discloses a preparation method of the MMLV reverse transcriptase mutant and application of the MMLV reverse transcriptase mutant in reverse transcription reaction.

The invention relates to a preparation method and application of shape memory organic hydrogel with heterogeneous phase change, and belongs to the technical field of material scientificity, the organic hydrogel is prepared through a one-step emulsion thermal polymerization method, and the organic hydrogel not only shows remarkable stretchability under the phase change exceeding 1100%, but also has good shape memory performance under the phase change exceeding 1100%. The prepared electromagnetic shielding material has the advantages of excellent mechanical property, excellent stability, excellent thermal mechanical property, excellent recoverability, good shape memory effect, excellent electromagnetic absorption capability and electromagnetic shielding performance with absorption as a main component.

The invention discloses an ROS-responsive prostate cancer targeting prodrug as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The prodrug is formed by covalently linking a prostate cancer targeting peptide SYEELR, a ROS (reactive oxygen species) response type ketal thiol (TK) connexon and an active molecule Devimistat. The preparation method comprises the following two steps: firstly, coupling Devimistat with a TK linker to obtain an intermediate Dev-TK, and then coupling the intermediate Dev-TK with an SYEELR peptide fragment to obtain a target product. The prodrug can specifically break and release Devimistat in a tumor high active oxygen microenvironment, and active targeting delivery is realized by virtue of the SYEELR peptide. The invention provides a novel prostatic cancer treatment strategy with accurate targeting, controllable release, enhanced curative effect and toxicity reduction potential.

The invention relates to the technical field of optical fiber communication, and discloses a clock recovery system and method for a multi-aperture coherent optical signal, and the system comprises an interpolator which obtains coherent optical signals received through a plurality of different apertures, and carries out the preliminary adjustment of the clock phase of the coherent optical signals; the MIMO equalizer equalizes and synthesizes a plurality of coherent optical signals of which the clock phases are readjusted, and the error detector tracks the clock phases of the transmitted coherent optical signals in real time and extracts the clock phase error of each coherent optical signal relative to the transmitted coherent optical signals in the synthesized coherent optical signals. The clock generation chip is used for generating a reference clock with a corresponding clock phase according to the clock phase error of each coherent optical signal, so that the clock phase of each coherent optical signal is readjusted through the reference clock, and at the moment, the coherent optical signals with the readjusted clock phases are balanced and synthesized; the quality of the synthesized multi-aperture signal can be improved.

The invention discloses a catechol side group-containing polylysine derivative as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving catechol acid in an organic phase, firstly adding NHS, stirring and dissolving until the materials are uniformly mixed, then adding EDC.HCl, and stirring and reacting to obtain a catechol-NHS intermediate solution; (2) dissolving polylysine or a salt thereof in a water phase, and adjusting the pH value to 6.0-7.0 by using an alkaline solution to obtain a solution of polylysine or a salt thereof; and (3) adding the intermediate solution obtained in the step (1) into the polylysine or the salt solution thereof obtained in the step (2), and maintaining the pH value of the reaction system at 5.0-6.0 by using an alkaline solution to obtain the catechol side group-containing polylysine derivative. The preparation method is short in reaction time and mild in reaction condition, and the obtained product is high in grafting rate and high in raw material conversion rate and has a good application prospect in the aspects of tissue adhesives and the like.

The invention relates to the technical field of enzyme engineering, and discloses a pantothenic acid synthetase mutant and application thereof. According to the invention, site-specific mutagenesis is carried out on 62nd, 123rd and 189th amino acids of pantothenic acid synthase from escherichia coli, and site-specific and directed mutagenesis is carried out through an alanine scanning strategy near an active pocket to change protein amino acid residues; the 62nd phenylalanine is mutated into isoleucine and / or the 123rd amino acid arginine is mutated into glutamine and / or the 189th arginine is changed into isoleucine, so that the efficiency of catalyzing the D-panthenol by the pantothenic acid synthetase is improved, and the yield of the D-panthenol is increased. Finally, when the sites are mutated at the same time, recombinant escherichia coli of the pantothenic acid synthetase can improve the efficiency of catalyzing synthesis of D-panthenol by the pantothenic acid synthetase by 1.32 times, and the yield of a genetic engineering strain carrying the constructed pantothenic acid synthetase mutant in shake flask fermentation of panthenol is improved by 3.65 times.

The invention provides a continuous production method of 2-acrylamido-2-methylpropanesulfonic acid and application of the 2-acrylamido-2-methylpropanesulfonic acid. The continuous production method comprises the following steps: S1, adding part of acrylonitrile into a reaction kettle in advance; s2, continuously adding the residual acrylonitrile into the mixing kettle, adding a pre-prepared fuming sulfuric acid solution, mixing, and controlling the mixing temperature at-10-10 DEG C to obtain a first mixed solution; s3, continuously adding the first mixed solution obtained in the step S2 into a supergravity reactor, gasifying isobutene at a constant speed, and introducing the gasified isobutene into the supergravity reactor for reaction to obtain a second mixed solution containing 2-acrylamido-2-methylpropanesulfonic acid; and S4, adding a second mixed solution containing 2-acrylamido-2-methylpropanesulfonic acid into the reaction kettle, continuously reacting, and after the reaction is finished, filtering and drying to obtain the 2-acrylamido-2-methylpropanesulfonic acid. The 2-acrylamido-2-methylpropanesulfonic acid prepared by adopting the production method is high in purity and high in product synthesis efficiency, and meanwhile, rectifying still residues generated in the reaction process are relatively low.

The invention belongs to the field of lipid delivery carriers, and particularly relates to a cationic lipid compound as well as a preparation method and application thereof. The cationic lipid compound is designed and synthesized, the lipid nanoparticle composition formed by the cationic lipid compound has the advantages in at least one aspect of the aspects of encapsulation efficiency, biological safety, transfection efficiency protein expression in cells and organisms and the like, and the problems existing in the prior art are solved. The method has non-important significance on development of gene therapy products. In order to improve the drug delivery effect of a gene vector and simplify the synthesis steps of the gene vector, the invention provides a cationic lipid compound as well as a preparation method and application thereof. The cationic lipid compound has positive electricity and can enter cells more easily, so that drugs are better delivered, a better treatment effect is generated, and the cationic lipid compound is simple to synthesize and easy to prepare and has practical application value.

The invention discloses a stereoisomer pair with near-infrared two-region luminescence property, a fluorescent probe as well as a preparation method and application of the stereoisomer pair. The structural general formula of the stereoisomer pair is as follows: # imgabs0 #, wherein X <-> comprises any one of ClO4 <->, BF4 <->, CF3COO <->, Cl <->, Br <->, I <-> and NO3 <->; and R1 and R2 independently comprise any one of # imgabs1 # and # imgabs2 #. The stereoisomer with the near-infrared second-region luminescence property shows obvious optical / physicochemical property difference in the aspects of absorption / fluorescence emission wavelength, fluorescence quantum efficiency and responsiveness, one of the stereoisomers shows high-sensitivity and high-specificity responsiveness in the physiological environment, and the other stereoisomer shows high-sensitivity and high-specificity responsiveness in the physiological environment. And the other isomer hardly has responsiveness. Meanwhile, the stereoisomer shows obviously different biomedical activities in the process of inducing pyroptosis of cells, and has wide application prospects in the fields of biological imaging, drug screening and development and the like.

The invention relates to the field of image generation, in particular to an AIGC-based advertising map generation method, which comprises the following steps of: periodically detecting an associated element proportion and an associated element complex coefficient of a target generated image in each target image combination so as to determine a combination state of each target image combination; determining an image analysis strategy according to the combination state; when an element combination analysis mode is adopted to extract optimal generation elements, the optimal generation elements are determined according to the reference evaluation coefficients, and the combination coefficients of the key analysis elements are adjusted; when the preferential generation elements are extracted in a preference analysis mode, the preferential generation elements of the target image combination are determined according to the reference evaluation coefficient and the reference use frequency; under the image generation condition, the target combination elements are determined according to the optimal indexes, the target element set is determined according to the reference combination coefficients, and the effectiveness of the image synthesis result and the image synthesis efficiency are improved.

The invention provides a photocathode for catalytic production of hydrogen peroxide, which comprises a conductive substrate and an organic polymer photocathode material film formed on the surface of the conductive substrate, and a monomer unit of an organic polymer contains a functional group capable of reducing oxygen into hydrogen peroxide through photoelectrocatalysis. The invention further provides a preparation method and application of the photocathode.

The invention provides a voice signal synthesis method and device, electronic equipment and a storage medium, and relates to the technical field of voice synthesis, the method predicts the amplitude spectrum and phase spectrum of a voice signal at a frame level, and avoids the periodic noise problem caused by an up-sampling structure with large calculation amount and transposition convolution. Moreover, according to the method, the frame-level fundamental frequency is obtained from the input voice signal Mel spectrum, the voiced sound frames and the non-voiced sound frames are determined, and the reference amplitude spectrum is generated in an energy information distribution mode, so that extra amplitude spectrum information can be provided for predicting the amplitude spectrum and the phase spectrum of the voice signal frame by frame, the prediction difficulty is reduced, and the prediction efficiency is improved. Therefore, the synthesis efficiency and quality of the voice signal are improved.