The invention relates to the technical field of crop seedling cultivation, in particular to a culture medium for saline-alkali tolerant cotton seedling cultivation. The culture medium comprises gardensoil, vermiculite, carbonized saw dust, peat soil, coconut shred fibers, wormcast, calcium bentonite, hydrolyzed polyacrylonitrile, organic acids, quick-acting fertilizers, probiotics flora, organicSAP and a humic acid water-retaining agent. The carbonized saw dust is a product of fresh saw dust subjected to dry distillation, condensate mixing and fermentation composting, and the quick-acting fertilizers contain alkali-hydrolysable nitrogen, rapidly available phosphorus, rapidly available potassium and compound trace element fertilizers; the organic acids include glacial acetic acid, citricacid, polymaleic acid and oxalic acid; the probiotics flora includes bacillus laterosporus, phosphorus-solubilizing bacteria, gram positive actinomycetes, lactobacillus and rhodopseudomonas palustris.The culture medium can be used as a seedbed medium for saline-alkali tolerant cotton seedling cultivation, and drought tolerance and saline-alkali tolerance of cotton can be improved after seedling transplanting.