78 results about "Bacillus spores" patented technology

Fertilizer compositions for plant production are described, comprised of decontaminated manure and Bacillus spores, preferably a humic acid derived from lignite and, optionally, one or more of N compounds, P compounds, K compounds, and combinations of two or more of these compounds. Preferred compositions are those wherein the ingredients are blended into an admixture resulting in a granular product. Other preferred compositions are those blended into an admixture resulting in a powdered product. Preferably, the ingredients are formed into hardened prills or pellets. Processes for production and use are also presented.

Compositions including an isolated Bacillus species, spores or an extracellular product of B. coagulans, suitable for topical application, for inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus are disclosed. Methods of inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus by topical application of compositions that include an isolated Bacillus species, spores or an extracellular product of a B. coagulans strain are disclosed.

The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.

Fertilizer compositions for plant production are described, comprised of decontaminated manure and Bacillus spores, preferably a humic acid derived from lignite and, optionally, one or more of N compounds, P compounds, K compounds, and combinations of two or more of these compounds. Preferred compositions are those wherein the ingredients are blended into an admixture resulting in a granular product. Other preferred compositions are those blended into an admixture resulting in a powdered product. Preferably, the ingredients are formed into hardened prills or pellets. Processes for production and use are also presented.

The biological functionality of living microbial spores is modified using phenotypic engineering to endow the resulting modified spores with novel functionality that extends the usefulness of the spores for a variety of practical applications including, for example, sterility testing, the release of active compounds, and cell-based biosensing systems. A preferred embodiment entails engineering Bacillus spores to acquire synthetic new functions that enable the modified spores to sense and rapidly transduce specific germination signals in their surroundings. The newly acquired functions allow the spores to perform, for example, as self-reporters of cellular viability, self-indicating components of cell-based biosensors, and in other analytical systems.

The present invention provides monoclonal antibodies which are highly specific for Bacillus spores. Also provided are peptides derived from those monoclonal antibodies. Both the antibodies and peptides are highly specific and can discriminate between spores of potentially lethal organisms such as Bacillus anthracis and other harmless but closely related bacilli and provide a very powerful tool in the construction of detection instruments as counter measures.

The invention relates to the field of culture waste treatment, in particular to a tetracycline degrading bacterium and application thereof to the livestock and poultry excrement composting treatment. The bacillus cereus can be used for tetracycline degradation; through external amplification culture, degrading bacterium liquid with the thalli number being 1.0*10<9 to 10> CFU / mL is obtained; the bacterium liquid is used in the livestock and poultry excrement composting treatment according to the solid-to-liquid ratio of a certain proportion; tetracycline residue can be effectively degraded; the ecological risk of the soil after the application of livestock and poultry excrement composting products can be favorably reduced or even eliminated.

The invention discloses a process for fermenting and producing a feeding enzyme complex preparation by using dextran waste liquid, and the product thereof. The fermentation production process uses strains of aspergillus niger (05012) and strains of bacillus spore subtilis as enzyme-producing strains for production; the dextran production waste liquid is blended and then used as a raw material of liquid fermentation; fermentation liquid of organic substances containing higher concentration thalli and enzymes and the like are obtained by filtering through an inorganic membrane; the solid fermentation process is adopted in the post-fermentation stage; agricultural byproducts such as wheat bran and the like are subjected to flash sterilization, used as fermentation raw materials, and subjected to solid fermentation in a ventilating fermentation tank; after the fermentation, the product of the feeding enzyme complex preparation is formed through the procedures of drying, crushing, granulating (for stabilizing the enzyme activity through the granulating process) and packaging. The process has the advantages of greatly reducing the treatment cost of the dextran waste liquid, and completely utilizing the dextran waste liquid to really realize the zero discharge of the production waste water of the dextran and improve the types and the activity of the enzymes in the fermentation products simultaneously.

The present invention relates to safe concentrated medical waste treating process and apparatus with high temperature steam. The treating process includes high temperature steam treatment of crushed medical waste, and purifying treatment of the treated tail gas. The treating apparatus includes a feeding set, a sealed automatic crushing set, a high temperature sterilizing chamber and a tail gas purifying set connected successively. The present invention ensures the medical waste treating effect in high temperature steam, has the economic performance of treatment considered at the same time, and adopts the heat resisting thermophilic fat bacillus spore as the indicating bacteria to reach the sterilizing rate not lower than 99.999 %.

The invention relates to an efficient heavy metal soil restoration agent. The efficient heavy metal soil restoration agent is prepared from the following raw materials in parts by weight: 3-7 parts of phosphate compounds, 9-16 parts of straw, 3-7 parts of medical stone, 12-20 parts of polyamide fiber, 5-8 parts of sodium bismuthate, 6-13 parts of sodium sulfide, 1.2-2.5 parts of Bacillus pasteurii spore powder, 6-25 parts of turf powder, 0.3-0.6 part of powdery bacterial powder carrier, 0.5-1.2 parts of fruit residues, 0.1-0.25 part of saw dust, 3-6 parts of calcium oxide and 2-5 parts of a magnesium-containing preparation. By virtue of the efficient heavy metal soil restoration agent, polluted soil can be effectively restored in a short time, heavy metals are inactivated, the soil restoration function is comprehensive and strong, and the soil environment can be effectively improved.

The biological functionality of living microbial spores is modified using phenotypic engineering to endow the resulting modified spores with novel functionality that extends the usefulness of the spores for a variety of practical applications including, for example, sterility testing, the release of active compounds, and cell-based biosensing systems. An embodiment entails engineering Bacillus spores to acquire synthetic new functions that enable the modified spores to sense and rapidly transduce specific germination signals in their surroundings. The newly acquired functions allow the spores to perform, for example, as self-reporters of cellular viability, self-indicating components of cell-based biosensors, and in other analytical systems. Also disclosed are methods for testing adequate sterility of a system by using engineered spores.

The invention relates to a preparation method of bacillus spores. The preparation method comprises the following steps: inoculating 1 part of bacillus stearothermophilus into 10 parts of a nutrient-rich culture medium, and carrying out culturing for 12h at the temperature of 50-55 DEG C and the speed of 150-250 rpm / min; adding with 90 parts of the nutrient-rich culture medium, and carrying out culturing for 12h, thus obtaining nutritive cell culture fluid; adding 1 part of an inorganic ion culture medium to 1 part of the nutritive cell culture fluid, and carrying out culturing for 12h at the temperature of 55-60 DEG C and the speed of 100-150 rpm / min; and adding 1 part of the inorganic ion culture medium every 12h till the ratio of the total parts of the added inorganic ion culture medium to the nutrient-rich culture medium is 7:1 to 10:1, thus obtaining the culture fluid containing the bacillus spores. According to the scheme, only two culture media are needed, the operation is simple, and the repeatability is good; the culture time is shortened, and the consumed time is only 60-72 h; the yield of the spores achieves 10<9> / ml, and the spore rate is 90% or above.

The invention provides a Bacillus edaphicus CGMCC No.2315 which is high in salt tolerance, heat resistance and adversity-resistance, can secrete various phytohormones such as indopropionic acid, zeatin, gibberellin, and the like, and can control nematodes and various fungal diseases. The invention also provides a method for producing the spore powder of the Bacillus edaphicus, which effectively reduces the death rate of the core bacterial strains, enables the survival rate of the core bacterial strains to reach 93 percent above and prolongs the guarantee period of the spore powder to more than 12 months. The multi-functional bacterial strain and the production method of the spore powder thereof are suitable for producing biological organic fertilizer, ecological formula fertilizer, biological pesticide, soil improvement agent and other biological preparations.

The present invention is based on the provision of non-pathogenic Bacillus spores comprising: (i) a polynucleotide sequence encoding a phagosome membrane-rupturing agent; and (ii) a polynucleotide sequence encoding at least one further heterologous polypeptide. These may be used to deliver heterologous polypeptides to cells and in particular to phagocytic cells. Pharmaceutical compositions, vaccines and medicaments comprising the spores are provided and may be used for a variety of purposes including in immunisation and vaccination.

The invention discloses a preparation method and application of a cement-based material additive. The preparation method comprises the following steps: preparing a calcium chloride solution and a sodium bicarbonate solution with equal mass to prepare porous calcium carbonate microspheres; inoculating bacillus lysine into a culture medium, and adding a spore efficient transforming agent into a culture solution; putting the calcium carbonate microspheres into a lysine bacillus spore culture solution, and fully standing until the concentration of the spore culture solution tends to be stable so as to obtain calcium carbonate microspheres loaded with microbial spores; dissolving the calcium carbonate microspheres loaded with the microbial spores into a mixed solution of sodium alginate and barium silver, uniformly stirring, and coating the surface layers of the calcium carbonate microspheres through spray drying. Results show that the cement-based material additive prepared by the method is suitable for maritime work environments, can effectively fill and block cracks of cement-based materials, improves the durability of engineering and prolongs the service life of the engineering.

The invention relates to optimization of a methyl nutritional type bacillus spore-production fermentation medium. The fermentation medium consists of the following components: 0.5-0.6% of corn meal, 0.08-0.2% of corn starch, 0.9-1.2% of bran, 0.18-0.54% of glucose, 0.05-0.15% of soybean cake powder, 0.16-0.2% of soybean peptone, 0.005-0.006% of KH2PO4, 0.006-0.008% of (NH4)2SO4, 0.004-0.005% of MnSO4, 0.0005-0.002% of CaCO3, 0.005-0.006% of MgSO4.7H2O, and the balance of water. The culture medium provided by the invention not only can meet the requirement of a shake flask fermentation experiment but also can successfully amplify the result of shake flask fermentation to a fermentation tank. Compared with the culture medium before optimization, the spore conversion rate and the spore yield of the formula of the culture medium are improved by 1-10 times, and the formula of the culture medium adopts low-price agriculture byproducts, so that the production cost is greatly reduced, and a foundation is laid for large-scale industrial production of wettable powder.

The invention provides a bacillus promoter for wastewater treatment and a preparation method thereof. The method is implemented by taking fruits as a fermentation substrate through the steps of material preparation and pretreatment; primary bacteria cultivation, and prior fermentation; secondary bacteria cultivation, and medium-term fermentation; coarse filtration, and late fermentation; deploying, and curing; fine filtration, concentration, filling and the like, so that a finished promoter product is obtained finally. The promoter provided by the invention can make bacilli become dominant bacteria in a biochemical reactor for sewage treatment, maintain the high activity of bacilli, and provide necessary trace elements for the germination and formation of bacillus spores.

The present invention relates to peptides that bind to the bacterial spores, such as B. anthracis, B. subtilis and B. cereus spores. The present invention also relates to method of identifying such peptides using phage display ligand screening system. The present invention further relates to the use of such peptides for the detection of bacterial spores, such as Bacillus anthracis spores.

The invention discloses an insect-resistant medicament capable of controlling amaralin biological sources of corn borers, which comprises the following components in percent by weight: 5-9% of amaralin, 2-5% of matrine, 4-8% of citrus oil, 35-45% of beauveria bassiana spore solution and 40-50% of bacillus cereus spore solution. The insect-resistant medicament preferably comprises the following best components in percent by weight: 7% of amaralin, 3% of matrine, 5% of citrus oil, 40% of beauveria bassiana spore solution and 45% of bacillus cereus spore solution. Through actual field planting, effects of effectively inhibiting corn borer eggs and larvae as well as expelling imagoes can be achieved, and crops can be induced to generate protectin, thereby promoting the robust growth of the crops and improving the quality of the crops.

The invention discloses a preparation method of probiotics for feed, and belongs to the technical field of microorganisms. In order to solve the problems of limited effect and high manufacturing process cost of probiotics for the existing feed, the method comprises the following steps: preparing a culture medium and sterilizing; inoculating a fermenting agent; carrying out fermentation culture for24-48 hours; enriching the thalli and suspending the thalli into a bacterial suspension by using normal saline; preparing a protective agent; carrying out heat stress treatment on the bacterial suspension; and spray drying with a fluidized bed: adding the bacterial liquid added with the protective agent into a spray fluidization dryer for drying to obtain the mixed bacterial preparation. Mixed fermentation culture of the strains is carried out by utilizing the demand difference of the strains on oxygen. The probiotics for the feed are prepared on the basis of a mixed bacteria fermentation technology, a heat stress technology and a spray drying zone fluidized bed technology. Compared with a preparation method of probiotics for a traditional feed, the preparation method disclosed by the invention has the advantages that the types of the probiotics are enriched, the synergistic effect among strains is promoted, the formation of bacillus spores is promoted, and the viability of thalli isimproved.

The invention relates to the technical field of biological bacterial fertilizers, in particular to a growth-promoting biological bacterial fertilizer for saline-alkali land. The preparation of the fertilizer comprises the steps of: A, production of a compound microbial agent; and B: preparation of the growth-promoting biological bacterial fertilizer: mixing the compound microbial agent prepared inthe step A, turf soil, urea, a calcium magnesium phosphate fertilizer, vermiculite, potassium sulfate and attapulgite according to a proportion for 1.5 to 2 hours under stirring, performing granulation, drying and sieving to obtain the grwoth-promoting biological bacterial fertilizer for saline-alkali land. Through the above technical scheme, the compound microbial agent is obtained by performingliquid fermentation and solid fermentation to prepare Trichoderma asperellum solid inoculum, Bacillus subtilis solid inoculum and Bacillus licheniformis solid inoculum, mixing the three solid inoculaaccording to a ratio of 1:1:1 and performing complete crushing; and then the growth-promoting biological bacterial fertilizer for saline-alkali land is prepared by adding the turf soil, the urea, thecalcium magnesium phosphate fertilizer, the vermiculite, the potassium sulfate and the attapulgite into the compound microbial agent and performing mixing under stirring, granulation, drying and sieving. The growth-promoting biological bacterial fertilizer for saline-alkali land achieves a Suaeda glauca survival rate of 95% or more through a Suaeda glauca planting growth-promoting experiment in saline-alkali land.

The invention discloses a bacillus pasteurii and bacillus agglutinans combined soil solidification method, which comprises the following steps of bacillus pasteurii bacterium liquid and bacillus agglutinans bacterium liquid providing, sand sample mineralization and sand sample solidification. The bacillus pasteurii is used for inducing calcium carbonate to be precipitated into pores of sand particles; primary solidification is performed; then, exopolysaccharides secreted by the bacillus agglutinans in the life cycle are used for being continuously filled into the pores of the sand particles for secondary solidification; the solidification capability is enhanced; good study and application values are realized. By using the bacillus pasteurii and bacillus agglutinans combined soil solidification method, inside particle connection of the solidified sand is compact; the bonding effect is better; the cohesive force is increased. After the combined soil solidification method is used for solidifying the sand, the whole strength of the solidified sand is high. In addition, the combined soil solidification method has the characteristic that the brittleness damage generated by the single soil solidification by the bacillus pasteurii is avoided.

The present invention relates to peptides that bind to the bacterial spores, such as B. anthracis, B. subtilis and B. cereus spores. The present invention also relates to method of identifying such peptides using phage display ligand screening system. The present invention further relates to the use of such peptides for the detection of bacterial spores, such as Bacillus anthracis spores.

The invention discloses a strain domestication method for improving the bacillus spore production rate. The method comprises the following steps of activating bacillus; carrying out sub-culturing on the activated bacillus according to a sequence from a normal culture medium to a poor culture medium, and then increasing nutritive material concentration to culturing in the normal culture medium sequentially, wherein three culture media or more are set according to the concentration, and continuous passage is carried out for three generations or more. According to the strain domestication methodfor improving the bacillus spore production rate provided by the invention, the culture and domestication time is shortened to a great extent, the method is simple to operate, and the bacillus spore production rate can be improved to 96 percent according to the method provided by the invention.

The invention relates to the technical field of crop seedling cultivation, in particular to a culture medium for saline-alkali tolerant cotton seedling cultivation. The culture medium comprises gardensoil, vermiculite, carbonized saw dust, peat soil, coconut shred fibers, wormcast, calcium bentonite, hydrolyzed polyacrylonitrile, organic acids, quick-acting fertilizers, probiotics flora, organicSAP and a humic acid water-retaining agent. The carbonized saw dust is a product of fresh saw dust subjected to dry distillation, condensate mixing and fermentation composting, and the quick-acting fertilizers contain alkali-hydrolysable nitrogen, rapidly available phosphorus, rapidly available potassium and compound trace element fertilizers; the organic acids include glacial acetic acid, citricacid, polymaleic acid and oxalic acid; the probiotics flora includes bacillus laterosporus, phosphorus-solubilizing bacteria, gram positive actinomycetes, lactobacillus and rhodopseudomonas palustris.The culture medium can be used as a seedbed medium for saline-alkali tolerant cotton seedling cultivation, and drought tolerance and saline-alkali tolerance of cotton can be improved after seedling transplanting.

The invention relates to a fermentation method of bacillus. A culture medium adopted in a fermentation process comprises substances in parts by weight as follows: 150-300 parts of yeast fermentation waste, 3-6 parts of sodium chloride, 3-6 parts of corn flour, 0.3-0.5 parts of manganese sulfate monohydrate and 700-800 parts of water. The invention further relates to an application of bacillus spore powder prepared with the method. The bacillus spore powder is prepared from bacillus fermentation liquor obtained by fermentation and then compounded with other plant nutrients such as seaweed extract, finally, bio-organic fertilizer is obtained by compounding, the soil aggregate structure is greatly improved, a microbial environment of soil is improved, soil diseases are effectively prevented,and application quantity of pesticides is reduced; furthermore, investment of chemical fertilizer in China can be reduced, organic matter content of soil is obviously increased, biological activity ofsoil is improved, and the product has quite important effects of improving soil fertility, increasing crop yield, improving crop quality and enhancing stress resistance of crops.

The present invention provides a process for efficiently producing sporangia of Bacillus popilliae containing spores and parasporal bodies having controlling effects on Scarabaeidae insects, and a control agent and controlling method for Scarabaeidae insects obtained by said production process. In a process for producing sporangia of Bacillus popilliae containing spores and parasporal bodies by culturing Bacillus popilliae in a medium containing an adsorbent, the medium contains 0.2-4.0% by weight of glutamic acid.

The invention relates to the technical field of microorganisms, and in particular to a bacillus firmus inoculant. Each gram of the inoculant contains 1x10<10-12> viable bacillus spores; and the bacillus firmus is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC NO.1.2010. The inoculant can be used for preventing and controlling root-knot nematode disease in such crops as tobacco, banana, tomato and the like; and the inoculant is safe to human and animals, good in environmental compatibility, free from residue of micro toxicity and free from pollution, and drug resistance of harmful pest cannot be caused easily.

The invention relates to a method for purifying bacillus spores. The method includes the steps that the bacillus spores are washed with bacterium-free water and concentrated; the product is washed with double salt washing liquid; the product is incubated with an enzyme solution; the product is washed with a surfactant solution; the product is washed with an enzyme inhibitor solution; the product is washed again with bacterium-free water; the product is put into a water bath at the temperature of 80 DEG C and sterilized; the finally-obtained bacillus spores are suspended with the bacterium-free water and stored. According to method, the purity of the spores is greatly increased while the quantity of the prepared spores is guaranteed; by means of the purifying method, active substances such as thallus nutritive cells, medium residual substances and protease released by fracture nutritive cells can be effectively removed, and induced transformation of the substances for the spores is effectively avoided; long-stem storage of the spore suspension liquid can be better achieved; the technology is easy to operate, easy to magnify, small in attenuation, good in repeatability and easy to industrially produce.