35 results about "Bacillus spores" patented technology

The invention relates to a concrete anti-cracking self-repairing agent in the technical field of building materials, and aims to solve the problem that concrete cracks are difficult to self-repair. According to the technical scheme, the self-repairing agent is characterized by comprising a microbial repairing unit formed by ZSM-5 type zeolite molecular sieve loaded bacillus pasteurii spores, mesoporous silica particles loaded with L-calcium aspartate and Fe3O4 (at) SiO2 core-shell nanoparticles which are fixed through an in-situ polymerization coating layer to form a magnetic thermal response capsule layer coating the surface of the microbial repairing unit, pLGA microspheres containing yeast extract are positioned on an interface of zeolite and hydrogel, and a trehalose-anhydrous calcium chloride eutectic compound is used as a directional mineralization accelerant. The concrete anti-cracking self-repairing agent provided by the invention is mainly used for intelligent identification and self-repairing of concrete cracks, and efficient repairing is realized through a microbial mineralization, calcium ion slow release and magnetic thermal response synergistic mechanism.

This invention relates to a microbial mineralization composite material for inkjet 3D printing and its preparation method. The composite material comprises powder materials and a binder. The powder materials, by weight, include: 70-85 parts magnesium phosphate cement powder, 10-25 parts fine aggregate, 0.5-3 parts a porous protective carrier loaded with urease-producing Bacillus licheniformis spores, 0.05-0.5 parts a nutrient protectant, and 1-5 parts a calcium source. The binder, by weight, includes: 94-98 parts deionized water, urea, 0.02-0.2 parts nutrients, 0.01-0.5 parts a pH adjuster, 0.5-5 parts a viscosity adjuster, and 0.01-0.2 parts a surface tension adjuster. The concentration of urea in the binder is 0.02-0.10 mol / L. This invention solves the problems of existing inkjet 3D printing materials having internal pores, microcracks, and weak interlayer interfaces after molding, limiting the improvement of the mechanical properties of printed parts in the later stages, and the difficulty in adapting the microbial mineralization system to the inkjet 3D printing process.

The present invention is in the field of hard surface cleaning compositions. Particularly, it relates to an aqueous hard surface cleaning composition with a good environmental profile that remains stable on storage and provides efficient cleaning and hygiene benefit that sustain for a longer period. There is a need for an improved liquid hard surface cleaning composition with a good environmental profile that provides efficient cleaning and hygiene benefit which sustains for a longer period. It has been found that select Bacillus spore in combination with select copolymers and surfactant provide a storage stable composition. The spore remains viable for germination after storage under various condition and provides efficient cleaning and hygiene efficacy that sustain for a longer period.

The application belongs to the field of cosmetics, and particularly relates to a bacillus fermentation product for repairing and moisturizing, and a preparation method and application thereof. The preparation method of the bacillus fermentation product for repairing and moisturizing comprises the following steps: S1, culturing bacillus in a culture medium to obtain bacillus spores; S2, treating the bacillus spores obtained in step S1 with lysozyme and protease, and then treating the bacillus spores with an ultrahigh-pressure homogenizer to obtain a spore lysis product; and S3, centrifuging, concentrating and heating the spore lysis product obtained in step S2 to obtain the bacillus fermentation product for repairing and moisturizing. The spore lysis product is applied to the field of cosmetic raw material preparation, and the bacillus fermentation product obtained by the application has excellent repairing and moisturizing effects, can inhibit staphylococcus aureus, and has the effect of balancing skin microecology.

The invention provides a prebiotic milk tea solid beverage and a preparation method thereof. Comprising the following steps: (1) preparing fiber mesh powder coated with probiotic spores; (2) preparing immobilized yeast gel microspheres; (3) drying the gel microspheres in a fluidized bed, and spraying a coating solution until the weight of the microspheres is increased by 18% to obtain immobilized yeast microspheres; (4) mixing the fiber mesh powder coated with the probiotic spores, the immobilized yeast microspheres, MCT powder and vanillin to obtain mixed powder; and putting the mixed powder into a fluidized bed, spraying a binder, and granulating until the particle size is 200-300 microns and the water content is 4.3-4.5%, thereby obtaining the prebiotic milk tea solid beverage. According to the invention, four functional factors of prebiotics (GOS / FOS), probiotics (lactobacillus spores and yeast), high protein and tea polyphenol are integrated, and 1 + 1gt among the functional factors is realized through cascade reaction of yeast metabolism, spore activation and bacterial enzyme synergism; 2, through the synergistic effect, the intestinal flora balance and the body metabolism health are jointly regulated;

The invention relates to the technical field of environmental sludge treatment, in particular to sediment environment in-situ remediation particles and a preparation method thereof. The soil conditioner is prepared from the following raw materials: zeolite powder, volcanic rock powder, bentonite, silica sol, a functional component loaded precursor and bacillus spore powder. The sediment in-situ remediation particles are of a core-shell structure, activated zeolite-volcanic rock serves as an inner core to construct a porous framework, Fe / Mn and Mg / Ca loading sites are combined, pollutant adsorption, fixation and passivation are achieved, the sediment microenvironment is improved, conditions are created for outer-layer bacillus bioremediation, and space-time synergy of physicochemical and bioremediation is formed; a preparation route of curing nucleation and then low-temperature coating is adopted, the contradiction between mineral roasting and microorganism temperature intolerance is solved, the spore activity is guaranteed, and the loss resistance of the spore is improved.

Process for detecting gram-negative bacteria and / or fungi contaminants in a detergent comprising gram-positive bacteria comprising the subsequent steps of: Providing a detergent comprising intentionally added spores of Bacillus sp; wherein the amount of spores is from 1x102 to 1x1014 CFU / g of detergent; and Adding a sample of the detergent to a liquid growth medium, wherein the medium comprises an effective amount of gram-positive bacteria selective growth inhibitor, and wherein the medium comprises an effective amount of disinfectant neutralizer to provide a liquid growth mixture; and Incubating the liquid growth mixture for from 6 hours to 48 hours at a temperature of from 18 to 46 degrees Celsius; and Diluting the growth mixture by from 3- to 100-fold to provide a liquid growth mixture dilution; and Lysing cells present in the liquid growth mixture dilution; and Applying an ATP-dependent bioluminescence assay to determine the amount of gram-negative and / or fungi contaminants in the detergent provided at step a.

The application relates to the field of improving severely salinized soil, and discloses a soil improver and a preparation method and application thereof. The method comprises the following steps: mixing agricultural and forestry wastes and bacillus spores, and sequentially carrying out anaerobic composting and aerobic composting, wherein the agricultural and forestry wastes are a mixture of at least one of corncob, mushroom residue, peanut shell and crop straw and sweet sorghum residue. The composition provided by the application not only has the functions of water and fertilizer conservation, soil fertility improvement, soil structure improvement and biological diversity improvement, but also can promote plant growth, reduce the total salt content, pH value, alkalinity, conductivity and sodium ion exchange capacity of the soil.

Particulate laundry additive composition having a plurality of particles wherein each of said particles has a mass between about 1 mg to about 5000 mg, the composition including:i) at least about 60% by weight of the composition of particle A, wherein particle A includes at least 40% by weight of the particle of a carrier, from about 0.1% to about 10% by weight of the particle of perfume and the particle is substantially free of Bacillus spores and enzymes; andii) at least about 2% by weight of the composition of particle B, wherein particle B includes at least about 40% by weight of the particle of a carrier, from about 0.0001% to about 5% by weight of the particle of Bacillus spores and enzymes and less than about 0.01% by weight of the particle of perfume.

The present invention provides an improved low-pH fabric care composition that provides freshness and odor prevention effects even after the fabric has been washed, i.e., during storage and use of the fabric. [Solution] A liquid fabric care composition comprising an organic acid and / or a salt thereof, a Bacillus spore, an aromatic material including an aldehyde-based fragrance raw material, and water, wherein the composition comprises less than 10% by weight of the composition, selected from the group consisting of cleaning surfactants, bleaching agents, fabric softening materials and mixtures thereof, the composition has a stock pH of about 2 to about 4, the composition is added during the rinse cycle of a laundry process, and the composition comprises 0.001% to 1.0% by weight of a sulfur-containing compound selected from sulfate compounds, bisulfate compounds or combinations thereof.

The present disclosure relates to laundry additives comprising a perfume, bacterial spores, and an enzyme. A particulate laundry additive composition having a plurality of particles wherein each of the particles has a mass of between about 1 mg to about 5000 mg, the composition comprising: i) at least about 60% by weight of the composition of particles A, wherein particle A comprises at least 40% of a carrier by weight of the particle, from about 0.1% to about 10% of a perfume by weight of the particle, and the particle is substantially free of Bacillus (Bacillus) spores and enzymes; and ii) at least about 2%, by weight of the composition, of a particle B, wherein the particle B comprises at least about 40%, by weight of the particle, of a carrier, about 0.0001% to about 5%, by weight of the particle, of bacillus spores and enzymes, and less than about 0.01%, by weight of the particle, of a perfume.

The invention relates to the field of microorganisms, in particular to a method for delaying spore formation of bacillus and application. Sodium selenite is added into a culture medium in the initial logarithmic growth stage of bacillus fermentation culture, so that the formation of spores is delayed; the concentration of the selenite is 0.01 to 0.5 mM. The method achieves a good effect in the treatment or prevention of ulcerative colitis, colorectal cancer and intestinal stress. The invention not only provides a basis for exploring a molecular mechanism of selenium-regulated spore formation, but also verifies that the survival competitiveness and the treatment efficiency of the probiotics can be improved through simple culture medium optimization, and provides a feasible new strategy for enhancing the curative effect of a probiotic therapy in intestinal diseases such as ulcerative colitis and the like.

The present invention relates to an aqueous fabric cleaning composition. Particularly, it relates to malodour reduction from soft surfaces like fabric which may cleaned using a laundering process to deliver this benefit. This has been achieved through a fabric cleaning composition comprising a combination of a bacillus bacterial spore and select chelating agent in an aqueous surfactant containing composition.

The present invention provides a method for producing a fermentation product, comprising culturing recombinant exospore-producing Bacillus cells that express a fusion protein of interest in exospores in a medium containing a carbon source and a nitrogen source at a total concentration of 20 g / L or more, to obtain a fermentation broth containing a high titer of recombinant Bacillus spores.

The present invention relates to a self-repairing shrinkage-reducing water reducer and its preparation, and recycled aggregate concrete. The water reducer comprises the following raw material components in parts by weight: 150 to 250 parts of water reducer; 10 to 50 parts of air-entraining agent; 20 to 100 parts of retarder; 50 to 100 parts of alkaline powder; 40 to 120 parts of calcium lactate; 20 to 60 parts of rice husk ash; 50 to 100 parts of dry powder of aerobic alkali-tolerant Bacillus spores; 50 to 100 parts of auxiliary agent; 20 to 80 parts of dispersant; 150 to 200 parts of solvent; 400 to 600 parts of water. The shrinkage-reducing water reducer of the present invention plays a role in optimizing the properties of recycled aggregates, reducing the influence of mud content and old mortar on the workability of recycled concrete, thereby improving the fluidity of concrete, etc.; in addition, aerobic alkali-tolerant Bacillus spores use rice husk ash as a bio-based carrier, which can achieve a good self-repair effect, can effectively fill the cracks and defects in recycled aggregates, bridge the concrete on both sides of the cracks and the fiberglass of recycled fan blades, significantly enhance the bonding force in the crack area, and effectively relieve the stress concentration problem caused by shrinkage stress.

Methods and systems for pretreatment of organic waste prior to large-scale production of lactic acid from the organic waste are provided, which employ dried or partially-dried compositions of Bacillus coagulans spores. The methods and systems advantageously provide partial conversion of the organic waste to L-lactic acid already at the pretreatment stage, thereby producing an improved feedstock material for large- scale production of L-lactic acid.

The application discloses a modified zeolite powder-based sediment repairing agent, which comprises 25-35 parts of zeolite powder, 15-25 parts of yellow sand, 15-20 parts of calcium peroxide powder and 15-25 parts of a microbial agent carrier complex in terms of mass fraction; the zeolite powder contains neodymium elements; and the microbial agent carrier complex contains carbon powder, attapulgite, bacillus spores, EM bacteria, nitrifying bacteria and denitrifying bacteria. The application further discloses a method for preparing the above sediment repairing agent.

Provided are compositions derived from Beauveria bassiana, Brevibacillus brevis, Streptomyces spororaveus, Paenibacillus elgii, or Streptomyces sp. fermentation, having algicidal properties, and method of use thereof.

The invention belongs to the technical field of agricultural microbial technology and traditional Chinese medicinal material cultivation, and particularly relates to a silicon dioxide-containing crocus sativus seed ball disease-resistant growth-promoting microbial coating agent and application thereof. The microbial coating agent is prepared from a nano silicon dioxide material and thalli or bacterial liquid of composite bacillus by taking chitosan as a film-forming agent and taking trehalose as a protective agent through physical mixing and ultrasonic dispersion processes. The nano silicon dioxide, the composite bacillus, the chitosan and a trehalose film forming system are combined to form a stable biological functional coating with good air permeability, and the biological functional coating has the effects of pathogen inhibition, growth promotion, field planting protection and the like, and is suitable for seedball disease prevention, quality improvement and green and efficient cultivation of saffron and other tuber medicinal plants.

The present invention relates to an oral composition comprising the superoxide dismutase (SOD) enzyme and / or Bacillus spp. strain spores, and a use thereof for improving oral health or managing oral hygiene. The composition may be useful for preventing or treating oral diseases such as oral pain, halitosis, tartar, plaque, gingivitis, and periodontitis, without causing adverse effects in the oral cavity.

The invention discloses a preparation method of an organic foliar fertilizer, which comprises the following steps: preserving the heat of fermentation liquor for producing single-cell protein thalli at 62-68 DEG C for 100-120 minutes, treating by using 18-20 Hz and 150-200 W ultrasonic waves, performing enzymolysis by using neutral protease and nuclease, and adding 0.1-0.3 wt% of chitosan, 0.1-1.0 wt% of nano humic acid and a trace element additive into the obtained supernate; and adding 0.05-0.20 wt% of dimethyl silicone oil and 0.1-0.2 wt% of polyglycerol ester, and carrying out high-pressure homogenization. Adding 0.10 to 0.15 weight percent of bacillus subtilis spores, and uniformly mixing; and adjusting the pH value to obtain the organic foliar fertilizer. According to the method disclosed by the invention, the supernate of the methylobacterium single-cell protein fermentation liquor is effectively utilized, and the comprehensive economic benefit is improved. The prepared organic foliar fertilizer is easy to adhere and absorb, and can improve the granular structure of soil.

The present invention relates to an aqueous composition for reduction of malodour particularly primary malodur from surfaces. It is especially useful for malodour reduction from soft surfaces like fabric which may be sprayed with the aqueous composition and allowed to remain thereon. Optionally such fabric may thereafter be laundered using any known laundering process to deliver enhanced malodour reduction benefits. This is achieved through use of an aqueous surfactant containing composition that comprises a bacillus bacterial spore along with select chelating agent.

The invention relates to a lateral flow immune nucleic acid detection sensor and a preparation method and application thereof.According to the sensor, fluorescent microspheres marked by nucleic acid aptamers serve as detection probes, a multiple signal amplification platform is combined, and the novel multiple signal amplification sensor for multiple fluorescent lateral flow immune nucleic acid detection is constructed. And high-sensitivity and high-specificity multiple quantitative amplification detection of the concentration of a target object is realized. Compared with traditional monoclonal antibody paired multiple immunochromatography detection, the fluorescent microspheres labeled by spore particle aggregation nucleic acid of bacillus and the fluorescent microspheres labeled by single-chain binding protein (RPA1) are used as multiple signal amplification sensors, so that the signal intensity on a detection line is remarkably enhanced, and the sensitivity of the detection method is greatly improved.

The present invention relates to a composition for cleaning a surface while controlling insects. The composition is useful on hard surfaces in home, such as those on appliances, furniture, floors, walls and toilets, as well as on soft surfaces, such as garments, interior decorations, bed sheets and blankets. The use of the composition on such a surface ensures long-lasting cleaning after surface cleaning and prevents insects from being attracted to such a surface for up to 24 hours. This is accomplished by the use of a cleaning composition comprising spores of bacteria of the genus Bacillus, selected essential oil ingredients, and a surfactant.

The invention belongs to the technical field of remediation of contaminated soil by microbial agents, and particularly relates to a vegetable bacillus X24-3 strain and application thereof. The vegetable bacillus strain X24-3 is classified and named as the vegetable bacillus strain, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.35229, and the preservation date is July 14, 2025. The invention further discloses a preparation method of the vegetable bacillus strain X24-3. The screened X24-3 bacterial strain can efficiently degrade phthalic acid esters in various environments, so that the X24-3 bacterial strain can be used for bioremediation of polluted soil, and the X24-3 bacterial strain is adaptive to actual farmland multi-component pollution scenes. The X24-3 strain provided by the invention is high in degradation efficiency, the remediation period is remarkably shortened, and a technical support is provided for efficient and green remediation of PAEs pollution of farmland soil.

The application provides a production process of recycled concrete, which comprises the following raw materials in parts by weight: cement 35-45 parts, recycled aggregate 120-130 parts, mixed fiber 11-13 parts, river sand 66-70 parts, modified glue powder 16-20 parts, water reducing agent 7-9 parts, water 30-40 parts and bacillus spore liquid 1-3 parts; the mixed fiber comprises basalt fiber, polyformaldehyde fiber and wood fiber in a mass ratio of 1:4-8:2-5; the recycled concrete of the application further improves the performance of the recycled concrete by adding effective components such as the mixed fiber, the modified glue powder and the bacillus spore liquid, solves the problems of large shrinkage rate and poor impermeability of the recycled concrete, improves the crushing index of the recycled aggregate, and further improves the compressive strength of the concrete.

This invention discloses a method for producing Bacillus coagulans spore powder, providing a high-concentration, high-quality spore powder production method for a heat-resistant, multifunctional, tea tree phosphate-solubilizing biocontrol Bacillus coagulans strain. This method can ferment and produce high-viable-spore, highly stress-resistant mature spore fermentation broth, high-viable-spore, centrifuged concentrated spore powder, and high-viable-spore, high-quality spore powder of strain PPX-13 (CGMCC No. 7.608). Preservation tests show that the PPX-13 high-viable-spore, high-quality spore powder product has a shelf life of one year when stored at low cost in a dark, room-temperature environment. Based on the established industrial-scale production process, it can be preliminarily estimated that each ton of high-viable-spore, highly stress-resistant mature spore fermentation broth can produce 8.7 kg of high-viable-spore, centrifuged concentrated spore powder and 392.81 kg of PPX-13 high-concentration, high-quality spore powder product, enabling large-scale industrial fermentation production of Bacillus coagulans spore powder.

The present invention belongs to the field of microbial technology, and specifically relates to an antagonistic composite bacillus agent for Gastrodia elata continuous cropping obstacle and its application. Specifically, the agent provided by the present invention is a microbial fermentation broth, and the microorganisms are Bacillus amyloliquefaciens SWFU000435 and Bacillus velezensis SWFU000440; the Bacillus amyloliquefaciens SWFU000435 is classified and named as Bacillus amyloliquefaciens Bacillus amyloliquefaciens , deposit number: CGMCC NO.33089; the Bacillus velezensis SWFU000440, classified and named as Bacillus velezensis Bacillus velezensis , deposit number: CGMCC NO.33088. Applying the microbial agent of the present invention can not only reduce the incidence of Gastrodia elata diseases, but also effectively increase the yield of Gastrodia elata and alleviate the economic losses caused by continuous cropping obstacles.

A surface treatment composition comprising: a) from about 1x102 to about 1x109 CFU / g of the composition of Bacillus spores; b) from about 0.01% to about 5% by weight of the composition of a cationic polyglucan.

The present invention is in the field of liquid cleaning compositions. There is a need for an improved liquid cleaning composition with a good environmental profile that provides efficient cleaning and hygiene benefit which sustains for a longer period. It has been found that select sequestrants and surfactants which also contain a Bacillus spore provides a stable liquid cleaning composition. The composition provides long lasting cleaning and hygiene benefit. The present invention further provides a method of cleaning a surface and use of select sequestrants for stabilizing a Bacillus bacterial spore in a surfactant containing liquid cleaning composition.