2163 results about "Nicotiana tabacum" patented technology

The present invention generally relates to tobacco and tobacco products having a reduced amount of nicotine and/or tobacco specific nitrosamines (TSNA). More specifically, several ways to make tobacco plants that have reduced nicotine and TSNA levels have been discovered. Embodiments include tobacco harvested from said tobacco plants, cured tobacco from said tobacco plants, tobacco products made with said cured tobacco and methods of making these compositions.

The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.

Disclosed herein are GNGN and G1/G2 antibodies that recognize and bind various FcRs and C1q. Also disclosed herein are glycan-optiminzed antibodies, predominantly of the GNGN or G1/G2 glycoform, with enhanced Fcγ receptor binding achieved through CHO, Nicotiana benthamiana and yeast manufacturing systems. Nucleic acids encoding these antibodies, as well as expression vectors and host cells including these nucleic acids are also disclosed herein. Methods and pharmaceutical compositions including the monoclonal antibodies are provided herein for the prevention and/or therapeutic treatment of viral infections, cancers and inflammatory diseases.

A method of modifying the content of certain chemical compounds in tobacco materials is provided, the method including treatment of a tobacco plant component with at least one probiotic. For example, the method may modify the asparagine content in tobacco materials, which can result in a modification in acrylamide production when the tobacco material is exposed to elevated temperatures. The type of tobacco plant component treated according to the invention can be a tobacco seed, a tobacco seedling, an immature live plant, a mature live plant, a harvested plant, or a portion thereof. Examples of probiotics include probiotic species of the genera bifidobacterium, lactobacillus, enterococcus, proionobacterium, bacillus, saccharomyces, streptococcus, and mixtures thereof. Smoking articles and other tobacco products including such probiotic-treated tobacco materials are also provided.

The invention discloses a hametz used for fermenting tobacco and applications thereof. The hametz is characterized by comprising three strains of Bacillus subtilis, lactobacillus and microzyme, the viable count ratio of which is 10 to 50: 2 to 30: 2 to 30. The hametz can ferment waste material of the tobacco or the flavor used for producing natural cigarettes by using the tobacco and can be used for tobacco fermentation. The flavor used for the natural cigarettes prepared by the hametz has a velvet fragrance and can remarkably increase the fragrance, reduce the unpurified gas and reduce the thrill as well when the flavor is used in the cigarettes, thus leading the smoke gas to be lingering and smooth. The hametz used for fermenting tobacco leaves can remarkably shorten the alcoholizing time and improve the quality of the tobacco leaves.

A method of modifying the content of certain chemical compounds in tobacco materials is provided, the method including treatment of a tobacco plant or portion thereof with at least one enzyme. For example, the method may modify the content of tobacco smoke toxicant precursors in tobacco materials, which can result in a modification in toxicant production when the tobacco material is exposed to elevated temperatures. The type of tobacco plant or portion thereof treated according to the invention can be, for example, a tobacco seed, a tobacco seedling, an immature live plant, a mature live plant, a harvested plant, or a plant derivative. Smoking articles and other tobacco products including such enzyme-treated tobacco materials are also provided.

The invention discloses Bacillus subtilis, which is named Bacillus subtilis. The Bacillus subtilis was collected in China General Microbiological Culture Collection Center on January 15th, 2009, with a collection number of CGMCC No.2843. The Bacillus subtilis strain disclosed by the invention is screened from tobacco leaf habitats. Experiments prove that the fermentation liquor of the strain has a strong inhibition effect on fungal pathogens such as Alternaria alternate and pepper anthracnose and demonstrates high prevention effect on Alternaria alternate, tobacco black shank, pepper anthracnose, tomato wilt, phytophthora melonis Katsura, verticillium dahlia and the like and has high growth promoting effects on tobacco, pepper, cucumber, tomato and eggplant. Therefore, the Bacillus subtilis disclosed by the invention can be used for preparing biological bactericides for preventing and controlling fungi in tobacco and vegetables and can be used for preparing growth promoting agents for promoting the growth of tobacco or vegetables.

The invention discloses an electronic cigarette liquid rich in plant flavone. The electronic cigarette liquid comprises the following raw materials in percentage by mass: 50-70% of propylene glycol, 5-10% of glycerinum, 5-15% of deionized water, 5-15% of natural flavone extract, 4-8% of tobacco extract, 2-6% of tobacco essence and 2-6% of thickener, wherein the natural flavone extract is the extract obtained by distilling by vapour or extracting a plant mixture by ethyl alcohol, and the total flavonoid content is 20-50% by total flavonoid content; the plant mixture is composed of chuzhou chrysanthemum, iris tectorum roots, propolis, orange peel, grape, peanut coat, lily, radix puerariae, scutellaria baicalensis, roxburgh rose and green tea. The electronic cigarette liquid disclosed by the invention is capable of meeting the smoking needs of smokers; meanwhile, the rich plant flavone has the effects of resisting oxidization, preventing aging, and adjusting immunity, and also has a healthcare effect on heart and cerebral vessels to a certain extent.

Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

The invention discloses a composition for preventing and treating virus diseases of crops, which is prepared from a medicament A for preventing and treating virus diseases, a medicament B for killing pests with piercing-sucking mouthparts, a plant growth regulator, a plant nutrient ingredient C, an assistant and a carrier. The medicament-fertilizer composition realizes multiple purposes, can prevent and treat virus diseases and control the main transmitting vectors of the virus diseases such as aphids, plant hoppers and the like, is capable of adjusting the growth of crops and complementing nutrition to crops, improves the disease resistance of crops while enhancing the yield and quality of crops, and can be used for preventing and treating virus diseases of crops including rice, tobacco, cucumbers, tomatoes and the like.

Four genes, A622, NBB1, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBB1, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.

A microbial preparation for preventing and treating the bacterial wilt, damping-off, etc of plant and promoting growth of palnt contains paenibacillus polymyxa or the fermented liquid obtained by culturing the bacterium.

The invention relates to the field of pesticides, in particular to ultralow volume liquid containing benzoylurea insecticide, which comprises active ingredients, solvent and adjuvant. The active ingredient I is optional one of lufenuron, diflubenzuron, triflumuron, chlorfluazuron and chlorbenzuron belonging to benzoylurea, and the active ingredient II is insecticide of another type of function mechanism. The ultralow volume liquid containing benzoylurea insecticide has the advantages of simplicity in processing, high efficiency, water saving, long-term effect, a target being not easy to resist the insecticide, low environmental pollution, synergy and the like. Application of ultralow volume spray, low volume spray or ultralow volume static spray is well effective in controlling ostrinia furnacalis, sugarcane borer, locust, peach fruit borer, lesser green leaf hopper, striped flea beetle, bean fly, diamond back moth, prodenia litura, cabbage caterpillar, leaf beetle, thrip, leaf miner, inchworm, red spider and the like, and the ultralow volume liquid containing benzoylurea insecticide can be used for effectively controlling various pests on wheat, maize, vegetables, tobacco plants, tea plants, fruit trees, cotton plants, woods and sugarcanes.

The invention relates to an application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary arbuscular mycorrhizal fungi fungicide is inoculated and propagated separately, and propagule spore density of an arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per gram of dry soil. A float breeding method is adopted to conduct arbuscular mycorrhizal fungi fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step inoculation, and the mixed fungicide is mixed by propagated acaulospora mellea, glomus mosseae and glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal fungus is close to a natural state, and fungus cooperate and play a better role of growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic system built during a seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.

A method for preparing a tobacco flavor extract from cured tobacco materials. The tobacco flavor extract has reduced level of tobacco specific nitrosamines (TSNAs) and/or nicotine. The tobacco flavor extract is suitable for use in an electronic smoking device to provide a tobacco taste for a user of the electronic smoking device. The method preserves volatile and semi-volatile flavor components that can be released during a low-temperature vaporization process occurring in the electronic smoking device.

The invention discloses a new technology for curing the red flower gold dollar tobacco leaf to be yellow and fragrant, belonging to the technical field of tobacco preparation. The technology comprises the following steps of: a first stage: (1) a leaf changing period: drying a ball at 37-38 DEG C and wetting the ball at 35-36 DEG C till that the leaf is turned to be yellow at 7-8 level and the leaf is softened and withered, and (2) a withering period: drying the ball at 42 DEG C and wetting the ball at 37-38 DEG C till that the leaf is turned to be yellow at 9-10 level and a main vein is soften; a second stage: (1) a vein changing period: drying the ball at 45-46 DEG C and wetting the ball at 37-38 DEG C till that the yellow leaf and the yellow vein are changed to be the status of a small winding drum, and (2) a period for curing the dried leaf to be fragrant: drying the ball at 52 DEG C and wetting the ball at 39-40 DEG C for 12+/-0.5h till that the dried leaf is changed to be a winding drum; and a third stage: drying the ball at 65 DEG C and wetting the ball at 40-42 DEG C till that the vein of the full baked tobacco leaf is dried. The new curing technology can obviously reduce the green smoke rate and the curing phenomena of the green vein, the green leaf, the floating green and the like, and can improve the fragrant quality and the appearance quality of the tobacco leaf.

Transgenic chloroplast technology could provide a viable solution to the production of Insulin-like Growth Factor I (IGF-I), Human Serum Albumin (HSA), or interferons (IFN) because of hyper-expression capabilities, ability to fold and process eukaryotic proteins with disulfide bridges (thereby eliminating the need for expensive post-purification processing). Tobacco is an ideal choice because of its large biomass, ease of scale-up (million seeds per plant), genetic manipulation and impending need to explore alternate uses for this hazardous crop. Therefore, all three human proteins will be expressed as follows: a) Develop recombinant DNA vectors for enhanced expression via tobacco chloroplast genomes b) generate transgenic plants c) characterize transgenic expression of proteins or fusion proteins using molecular and biochemical methods d) large scale purification of therapeutic proteins from transgenic tobacco and comparison of current purification/processing methods in E. coli or yeast e) Characterization and comparison of therapeutic proteins (yield, purity, functionality) produced in yeast or E. coli with transgenic tobacco f) animal testing and pre-clinical trials for effectiveness of the therapeutic proteins. Mass production of affordable vaccines can be achieved by genetically engineering plants to produce recombinant proteins that are candidate vaccine antigens. The B subunits of Enteroxigenic E. coli (LTB) and cholera toxin of Vibrio cholerae (CTB) are examples of such antigens. When the native LTB gene was expressed via the tobacco nuclear genome, LTB accumulated at levels less than 0.01% of the total soluble leaf protein. Production of effective levels of LTB in plants, required extensive codon modification. Amplification of an unmodified CTB coding sequence in chloroplasts, up to 10,000 copies per cell, resulted in the accumulation of up to 4.1% of total soluble tobacco leaf protein as oligomers (about 410 fold higher expression levels than that of the unmodified LTB gene). PCR and Southern blot analyses confirmed stable integration of the CTB gene into the chloroplast genome. Western blot analysis showed that chloroplast synthesized CTB assembled into oligomers and was antigenically identical to purified native CTB. Also, GM1,-ganglioside binding assays confirmed that chloroplast synthesized CTB binds to the intestinal membrane receptor of cholera toxin, indicating correct folding and disulfide bond formation within the chloroplast. In contrast to stunted nuclear transgenic plants, chloroplast transgenic plants were morphologically indistinguishable from untransformed plants, when CTB was constitutively expressed. The introduced gene was stably inherited in the subsequent generation as confirmed by PCR and Southern blot analyses. Incrased production of an efficient transmucosal carrier molecule and delivery system, like CTB, in transgenic chloroplasts makes plant based oral vaccines and fusion proteins with CTB needing oral administration a much more practical approach.

The invention discloses a green biological seedling raising matrix, relating to the seedling raising matrix technology. The preparation method comprises the following steps of: mixing straw powder, organic fertilizer and nutrient soil to be uniform, adding water until the water content is 45-55%, adding compound bacteria and then mixing to be uniform, stacking for fermenting, turning when the temperature increases to 50 +/- 2 DEG C, turning and cooling for 1-2 days when the temperature is increased to 50 DEG C again, adding microelements and growth hormone, stirring to be uniform, and packaging. The invention has the beneficial effects that: (1) the matrix is rich in organic matters and beneficial biological germs, has comprehensive nutrition and is loose and soft; (2) the matrix is seed-saving and labour-saving and can shorten seedling raising period; (3) the matrix can be used to ensure uniform and strong seedlings and high sprout rate; (4) the matrix can protect roots and hold water and fertilizer and has good air permeability; (5) the matrix has the functions of promoting flower bud differentiation and improving the yield; and (6) the matrix has the effect of resisting soil diseases. The matrix disclosed by the invention is applicable to seedling raising of vegetable, melon and fruit, flower and plant, tea, tobacco and cotton.

The invention provides a formula of a cured tobacco flavor, and pertains to the technical field of flavors. The formula of the cured tobacco flavor takes vanillin, isoamyl isovaleriate, aglaia odorate flower concrete extract, rhodinol and Maillard reactant as the main raw materials. The formula is applied to the tobacco to cause the cigarette to have a novel and nice fragrance, in particular to cause the cigarette to have a pure and mellow fragrance, thus obviously improving the impure fragrance and the smoke taste and causing the characteristic of the cigarette fragrance to be obvious. The formula has available flavor raw materials, and does not need to increase devices and change the production process. The addition amount is 1.2-2.5 per mill of the tobacco usually.

The microbial fertilizer is mixture of fermented liquids separately with photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria. It is prepared through inoculating photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria separately to the first stage culture medium, and culturing to obtain the first stage bacterial seeds; inoculating the first stage bacterial seeds separately to second stage culture medium, and culturing to obtain the second stage bacterial seeds; and inoculating the second stage bacterial seeds separately to the fermenting culture medium, and culturing to obtain fermented bacterial liquids; and mixing the fermented bacterial liquids in certain proportion to obtain the microbial fertilizer. The present invention also relates to the application of the microbial fertilizer in controlling diseases of tobacco and trees and the usage.

The invention relates to an overwintering preserving breeding method for an aphidius gifuensis and belongs to the technical field of biological control. The overwintering preserving breeding method for the aphidius gifuensis solves the problem that in the prior art, overwintering preservation is performed on the aphidius gifuensis difficultly, and the heavy demand for the aphidius gifuensis in a tobacco planting season in a coming year is difficult to meet. The overwintering preserving breeding method comprises a first step of greenhouse aphidius gifuensis selection which comprises cultivation of host plants, collecting of an aphidius gifuensis source, aphidius gifuensis inoculation in a greenhouse, and purification and rejuvenation; a second step of winter greenhouse aphidius gifuensis preservation which comprises cultivation of aphidius gifuensis preserving plants, aphidius gifuensis inoculation in the greenhouse, and purification and rejuvenation; and a third step of expanding propagation in the greenhouse. The aphidius gifuensis is successfully accumulated and preserved during the time from finish of massive reproduction of the aphidius gifuensis in August to April in a coming year and before massive reproduction of the aphidius gifuensis, and the aphidius gifuensises with enough quantity and good quality are provided for massive reproduction of the aphidius gifuensis in a next year.

The invention relates to phosphorus-solubilizing fungi and application thereof to preparation of a biological fungus fertilizer, belonging to the technical field of microbial fertilizers. The microorganism in the invention is a penicillium oxalicum Mo-Po strain which is preserved at the China General Microbiological Culture Collection Center on September 25th, 2010 with a collection number of CGMCC No. 4209. An ITS4 gene sequence of the Mo-Po strain is a main characteristic basis for identifying the strain, wherein the sequence number of the sequence is HQ703581 in a Gene Bank database. Tobacco waste can be thoroughly decomposed and converted into the biological fungus fertilizer by the microorganism with a composting fermentation method. The prepared microorganism has a good effect of promoting plant growth and can be used for substituting a chemical phosphorus fertilizer. The prepared biological fungus fertilizer has the characteristics of low cost, high fertilizer efficiency, no residue and safety to people and livestock and is a high-efficiency biological fungus fertilizer in organic agricultural planting.

The present invention relates to a novel tobacco cultivar designated AOB 171, which has low to intermediate nicotine content. The invention provides seeds of the cultivar AOB 171, plants and parts thereof of the cultivar AOB 171, a tissue culture derived from the cultivar AOB 171, hybrids produced from cultivar AOB 171 and lines derived from cultivar AOB 171, as well as genetically modified forms of the foregoing plants and tissue culture. Also provided are methods of producing cultivar AOB 171 plants, cultivar AOB 171 hybrid plants, and tobacco lines derived from cultivar AOB 171. In addition, products produced from the plants of the present invention are provided.

The invention provides a novel microbial fertilizer special for tobaccos. The fertilizer comprises the following components in parts by weight: 5 to 25 parts of a complex microbial inoculant and 75 to 95 parts of a carrier, wherein the complex microbial inoculant contains 1.6 to 8 hundred million cfu/g of bacillus subtilis, 1.6 to 8 hundred million cfu/g of bacillus licheniformis, 0.4 to 2.0 hundred million cfu/g of trichoderma harzianum and 0.4 to 2.0 hundred million cfu/g of calicheamicin streptomyces hygroscopicus; the carrier is a powdered material which are made by performing microbe fermentation and decomposition on rape seed cakes, bean pulp cakes and sesame-seed cakes, and then performing drying and sieveing. The microbial fertilizer contains bacterial strains for antagonizing fungus pathogenic bacteria, bacterial strains for preventing and curing the breeding of soil pathogenic bacteria, bacterial strains for improving the soil environment and the like. The carrier is fermented three-cake fertilizer. The invention also provides a preparing method for the microbial fertilizer. The microbial fertilizer special for the tobaccos, provided by the invention, is powder, is capable of absorbing the water and preserving the moisture. The fertilizer is mainly used as the base fertilizer when fertilizing, the soil fertility can be improved, the nutrient absorption of crops is improved, the diseases of a plurality of crops can be prevented, and the quality and the yield of tobacco leaves can be effectively improved.

The invention discloses a tobacco active biological organic-inorganic special fertilizer and a preparation method thereof. The tobacco active biological organic-inorganic special fertilizer is prepared by the following steps of: selecting functional microbial strain and adding the following materials in percentage by weight: 50-70 percent of rapeseed meal, 20-40 percent of sesame seed meal and 10-20 percent of bean meal; mixing, then performing solid fermentation and performing enzymolysis on crude protein in cake meal to form a biological fermentation cake fertilizer with bioactive free amino acid and active small peptide; and then uniformly mixing with an activator and an inorganic fertilizer and chelating to prepare the tobacco active biological organic-inorganic special fertilizer. The tobacco active biological organic-inorganic special fertilizer is characterized by comprising the following raw materials in percentage by weight: 8-12 percent of activator, 20-30 percent of biological fermentation cake fertilizer and 55-75 percent of inorganic fertilizer. According to the invention, a method of combining an organic fertilizer with an inorganic fertilizer and combining nitrate nitrogen with ammonium nitrogen is adopted, the biological fermentation cake fertilizer with the rapeseed meal, sesame seed meal and bean meal as carriers is added, and the raw materials are mixed with the activator and the like to prepare a high-efficiency active fertilizer. The fertilizer has the characteristics of high nutrition, high activity and high efficiency; the tobacco plant root environment can be improved, the root system activity is enhanced, the leaf growth is promoted, the requirements of tobacco plant development on mineral nutrition are fully met, the yield of the tobacco plant and the quality of the tobacco leaf can be obviously improved, the environment pollution is reduced, and the soil is improved.

The invention provides a preparation method of an essence for tobacco, particularly a preparation method composite extract for fermentation type tobacco, belonging to the technical field of tobacco. The preparation method comprises the following steps: (1) pretreating cherokee rose, wolfberry fruit and Tamarindus indica Linn, and preparing a culture medium; (2) inoculating: inoculating flavor-producing yeast into the culture medium; (3) fermenting; and (4) separating to obtain the composite extract. In the invention, cherokee rose, wolfberry fruit and Tamarindus indica Linn are used as raw materials to produce the composite extract for fermentation type tobacco with fruit fragrance by using microbial fermentation technology. When being adding into cigarettes, the composite extract for fermentation type tobacco can enhance the fragrance and sweetness in the mouth, soften the smoke, lower the irritation of cigarettes, improve the mouthfeel and reduce the residues. The microbial fermentation technology is utilized to produce the essence for tobacco, thereby obtaining a new economic, efficient and orientable way for producing essence for natural tobacco, and developing a novel essencefor tobacco, which can not be produced by the traditional essence production method.

The invention relates to a seedling stage identification method for the resistance of tobacco to the bacterial wilt. The seedling stage identification method comprises the steps of heat preservation pool construction, pool water heating and circulation, wilt bacterium inoculation, tobacco seedling, disease state investigation and grading. The method replaces a conventional field identification result with a seedling stage identification result, identifies a great amount of material in a smaller area, carries out the identification a plurality of times annually, improves the reliability of the result, shortens the identification cycle, saves the site and test cost, and covers little floor space. The seedling stage identification method for the resistance of tobacco to the bacterial wilt is characterized by rapid disease development, good result repeatability, and the like. The seedling stage identification method for the resistance of tobacco to the bacterial wilt can effectively remove the interference on the resistance result caused by soil temperature difference, strain pathogenicity difference, strain quantity difference and other diseases, thereby benefiting the recurrence and comparison of results from different years and different labs. The difference between the attack rates of infection resistant varieties and the disease indexes is obvious; and the appraisal result of the resistance of the variety is basically consistent with the perennial resistance appraisal result obtained by others. The method has greater application potential in the resistance identification and resistance heredity rule study of disease resistant resources and breeding medium materials.

The invention discloses scorch sweet aroma electronic cigarette juice which comprises, by mass, 50% to 70% of propylene glycol, 5% to 10% of glycerin, 5% to 15% of deionized water, 5% to 15% of natural scorch sweet aroma style spice, 4% to 8% of scorch sweet aroma cigarette extracts, 2% to 6% of cigarette essence and 2% to 6% of thickeners, wherein the natural scorch sweet aroma style spice is composed of fenugreek extracts, endive extracts, malt extracts, coffee extracts, flos farfarae extracts, daisy extracts, magnolia flower extracts and fructus momordicae extracts in a mixed mode. Smoke generated by the electronic cigarette juice is close to smoke of a traditional cigarette, the typical scorch sweet aroma characteristic is achieved, fragrance is mellow, aftertaste promotes saliva secretion, and demands of a smoker for the smoke can be met; in addition, the flos farfarae extracts, the magnolia flower extracts, the fructus momordicae extracts, the endive extracts and the malt extracts are common throat wetting and cough relieving medicine, and irritation of the smoke to the mouth, the throat and the lungs can be effectively reduced.

The invention discloses an isoflavone compound in a tobacco rhizome and a preparation method and application thereof. The preparation method comprises the following steps of: crushing a tobacco rhizome sample, performing ultrasonic extraction for 3 to 5 times by using 95 percent ethanol, combining the extracts, filtering, performing reduced pressure concentration to obtain an extract, primarily separating the extract by using silica gel column chromatography, further separating the extract by adopting high performance liquid chromatography, and thus obtaining the required new compound. Antibacterial activity screening test results show that the compound has strong antibacterial effect on common proteus species, escherichia coli, staphylococcus, bacillus subtilis and the like. The compound used for cigarette tipping paper plays an obvious role in inhibiting microbes for polluting the cigarette tipping paper.