2158 results about "Nicotiana tabacum" patented technology

The invention relates to the technical field of agricultural production data prediction, and discloses a tobacco plant number prediction method based on deep learning, and the method comprises the steps: collecting tobacco field environment data through a soil humidity sensor, a weather sensor, a laser radar point cloud sensor, a multispectral sensor and other multi-source agricultural sensors, carrying out the multi-source feature fusion through a dynamic feature selection network, and generating an environment feature vector. And inputting the model into a multi-modal time sequence prediction model adopting a space-time attention mechanism, and modeling a tobacco plant growth trend. And constructing an adaptive weighted regression model according to a prediction result, and dynamically adjusting the weight by using an improved particle swarm optimization algorithm. And constructing a hierarchical correction model to optimize prediction data. A real-time prediction updating method is provided, and newly-added data can be processed. The multispectral sensor data is subjected to dimension reduction, correlation modeling and interpolation fusion processing. According to the method, multi-source data is fully utilized, the accuracy, real-time performance and stability of tobacco plant number prediction are improved, and powerful support is provided for tobacco planting management.

The invention relates to the technical field of tobacco logistics and intelligent control, and discloses an intelligent temperature and humidity regulation and control system and method for tobacco transportation, and the system comprises a multi-dimensional environment sensing module, a self-adaptive strategy generation module and a closed-loop efficiency verification module. The method comprises the following steps: capturing three-dimensional space parameters in a transportation carrier in real time through distributed sensing nodes, outputting a structured environment situation matrix, and providing a space-time reference for a regulation and control decision; after receiving the environmental perception data, executing dynamic strategy optimization, establishing a transportation stage-spatial position two-dimensional regulation and control strategy library, processing sensitivity differences of tobacco leaves in different regions by adopting an asymmetric weighting algorithm, and generating a regulation and control scheme containing a gradient regulation instruction; real-time evaluation and strategy iteration of the regulation and control effect are achieved, microenvironment response detection is deployed, tobacco physical characteristic change data are collected, and a triangular verification model is constructed. The method effectively controls moisture content distribution, inhibits browning and mildew risks, and maximally retains tobacco leaf quality characteristics.

The present invention relates to the field of tobacco breeding, and more particularly to molecular markers for screening for spotted wilt-resistant tobacco plants free of linkage drag and their applications. A method for screening spotted wilt-resistant tobacco plants or germplasm with reduced linkage drag is provided, comprising: (a) screening for spotted wilt-resistant tobacco plants or germplasm and isolating nucleic acid thereof; (b) detecting a first linkage drag locus marker and / or a second linkage drag locus marker in the isolated nucleic acid; the first linkage drag locus marker comprising the NaChr4_2M marker shown in SEQ ID No. 10 and / or the NaChr4_8M marker shown in SEQ ID No. 11; the second linkage drag locus marker comprising the NaChr3_62.6M marker shown in SEQ ID No. 7 and / or the NaChr3_64.6M marker shown in SEQ ID No. 8; and (c) selecting a spotted wilt-resistant tobacco plant or germplasm that does not contain the first linkage drag locus marker and / or the second linkage drag locus marker. This method can be used to obtain spotted wilt-resistant tobacco plants free of linkage drag, providing superior varieties with high spotted wilt resistance for tobacco leaf production.

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.

The invention discloses a target segment for preventing and treating tomato brown fruit wrinkling virus in tobacco, double-stranded RNA (Ribonucleic Acid) and application of the target segment and the double-stranded RNA. Wherein the nucleotide sequence of the target segment is as shown in SEQ ID NO.3, or a sequence which is as shown in SEQ ID NO.3, has at least 90% of homology and has coded amino acid as shown in SEQ ID NO.4 is substituted. The invention also discloses a double-stranded RNA of a targeted interference target section, and the double-stranded RNA can significantly inhibit the proliferation of the tomato brown crinkled fruit virus in tobacco by injecting or smearing tobacco leaves, so that the double-stranded RNA can be effectively applied to prevention and control of tobacco diseases caused by the tomato brown crinkled fruit virus. The method for preventing and controlling the tomato brown crinkled fruit virus in the tobacco by using the double-stranded RNA is safe, harmless and efficient, and provides a new path for preventing and controlling plant virus diseases by using RNAi (Ribonucleic Acid Interference).

The invention relates to the technical field of inspection unmanned aerial vehicle flight control, and discloses an unmanned aerial vehicle inspection method and system for a tobacco field, and the method comprises the steps: obtaining multi-source remote sensing image data of a preset tobacco planting region; detecting tobacco plants in the remote sensing image data, and performing spatial clustering on detection results to obtain a plurality of tobacco plant cluster targets; and generating an initial inspection path according to the position coordinate and the vegetation index of the tobacco plant cluster target. And in the flight process, the flight pose and environmental parameters of the target unmanned aerial vehicle and the deviation angle between the unmanned aerial vehicle and the current path section are collected in real time. And when the adjustment condition is satisfied, automatically generating an improved path, and adjusting the flight attitude and the camera angle of the target unmanned aerial vehicle. And identifying whether an abnormal condition exists in the image data acquired in the flight process. The tobacco planting scene-oriented inspection method integrating multi-source remote sensing perception, adaptive path planning and intelligent identification is realized.

The invention discloses application of an NtTCP7 gene in cold resistance of tobacco, and belongs to the technical field of molecular biology. According to the application of the NtTCP7 gene disclosed by the invention in the cold resistance of the tobacco, the sequence of the NtTCP7 gene is as shown in SEQ ID NO. 1. The NtTCP7 gene negatively regulates and controls the cold resistance of the tobacco, and the cold resistance of the tobacco is remarkably improved by knocking out the gene. Excavation and functional identification of the gene provide important gene resources and theoretical basis for low-temperature-resistant breeding of tobacco.

The invention discloses a Trichoderma auritum GUWM22 strain and application thereof, and belongs to the field of microorganisms. The preservation number of the trichoderma auritum GUWM22 is CCTCC (China Center for Type Culture Collection) NO: M20242639, the preservation date is November 26, 2024, the preservation unit is China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China. Experiments prove that the GUWM22 has a very strong inhibition effect on six pathogenic bacteria (phytophthora nicotianae, phytophthora capsici, botryosphaeria dothidea, alternaria alternata, phomopsis and colletotrichum capsici), the bacteriostasis rate reaches 78% or above, in addition, the GUWM22 also has a good prevention and treatment effect on anthracnose of picked peppers (line peppers), the prevention effect is 82.11%, and it can be seen that the GUWM22 can be used for preventing and treating anthracnose of the picked peppers (line peppers). The Trichoderma aureum GUWM22 is a new biocontrol Trichoderma strain with good potential. The invention provides a new strain resource and an environment-friendly and efficient biocontrol approach for effective prevention and treatment of pepper anthracnose.

The invention belongs to the field of biological agents, and particularly relates to bacillus siamensis and application thereof in the aspects of plant disease control, growth promoting and yield increasing. The bacillus siamensis XNC-3 provided by the invention is wide in antibacterial spectrum, can effectively inhibit various plant pathogenic fungi including phytophthora parasitica var nicotianae, has broad-spectrum antibacterial activity, and can be used for preventing and treating plant diseases, especially tobacco diseases. The bacillus siamensis has a promoting effect on tobacco seedling growth and seed germination, has growth-promoting and yield-increasing effects on tobacco, can remarkably increase the tobacco yield and improve the tobacco quality, is safe to the environment and has a very good application prospect.

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention provides a large-scale tobacco field planting whole growth period automatic monitoring method and system based on unmanned aerial vehicle remote sensing. According to the method, an advanced image segmentation model and a target detection algorithm are fused, and automatic extraction of a tobacco field area, tobacco plant target identification, growth stage classification judgment and planting normalization verification are realized. Firstly, unmanned aerial vehicle remote sensing images covering the whole growth period of tobacco plants are collected; then automatically completing field segmentation through an image segmentation model, and extracting a tobacco field area; identifying the tobacco plants by using the target detection model and judging the growth stages of the tobacco plants; secondly, further judging whether a planting behavior exists in the target area or not, and identifying the number of tobacco plants by adopting a model in a'planted 'state; and finally, judging whether the plants conform to a standardized planting standard or not by combining regional distribution characteristics. The tobacco plant detection and management system has the characteristics of high automation and intelligence, the tobacco plant detection and management efficiency can be remarkably improved, and powerful technical support is provided for refined decision making and large-scale popularization of modern tobacco planting.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention relates to cloning of a stress-resistant gene LpbHLH144 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH144 of Lilium pumilum DC. Comprising the following steps: (1) cloning a target gene; (2) constructing an expression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH144 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the functions of overexpression of the LpbHLH144 for improving the saline-alkaline and drought stress of the tobacco are identified through transgenic tobacco, the functions of the lilium tenuifolium bHLH gene when the lilium tenuifolium bHLH gene is subjected to adversity stress are disclosed, a foundation is laid for further application of the lilium tenuifolium in lily breeding, and a theoretical basis is provided for the functions and effects of bHLH transcription factors.

The invention discloses a Brucella with antibacterial activity and application of the Brucella. The invention discloses Burkholderia sp.HNIV0701, and the preservation number of the Burkholderia sp.HNIV0701 is GDMCC No: 66007. The Burkholderia sp.HNIV0701 disclosed by the invention has a stable, efficient and broad-spectrum antibacterial property, has an inhibition effect on a plurality of plant pathogenic bacteria such as Botryosphaeria dothidea, Phytophthora nicotianae, Fusarium solani, Alternaria alternata, Thanatepora cucurbitae, Ralstonia solanacearum and the like, and also can be used for inhibiting Staphylococcus aureus and / or Listeria monocytogenes, so that the Burkholderia sp.HNIV0701 can be used for inhibiting a plurality of plant pathogenic bacteria such as the Botryosphaeria dothidea, the Phytophthora nicotianae, the Fusarium solanum, the Alternaria solanacearum and the like. The Brucella HNIV0701 can hydrolyze organic phosphorus and can be used for soil improvement. The Brucella HNIV0701 has nitrogen fixation capability and has the potential of promoting plant growth.

The invention relates to the technical field of quality detection of flue-cured tobacco leaves, in particular to an intelligent grading method, medium and system for appearance quality of flue-cured tobacco leaves, and the method comprises the steps: obtaining high-resolution multi-angle flue-cured tobacco leaf image data; noise in the image is removed through Gaussian filtering, and image quality is enhanced through histogram equalization; using a convolutional neural network to extract multi-level appearance features such as color, vein and integrity of the flue-cured tobacco leaves; carrying out binarization segmentation on the image, and extracting an accurate edge contour and leaf meridian features of the flue-cured tobacco leaves in combination with morphological operation; a deep convolutional neural network is utilized to fuse the multi-dimensional features, grading evaluation is carried out on the appearance quality of the cured tobacco leaves, and scores are output; the tobacco leaf local abnormal area identification method can be widely applied to quality detection in tobacco production, and has the advantages of high efficiency, intelligence and accuracy.

The invention discloses a key gene identification method related to tobacco nitrogen response, which comprises the following steps: S1, setting four nitrogen fertilizer gradient treatments on the basis of same phosphorus and potassium fertilization by adopting a field experiment of a completely random block; s2, randomly taking the 6th to 8th leaves of the five plants in each area, and dividing a sample into two parts: quickly freezing one part with liquid nitrogen, and storing at-80 DEG C for RNA (Ribonucleic Acid) extraction and transcriptome sequencing; one part is used for measuring the nitrogen content, and after baking and drying treatment, a KjeltecTM8100 automatic nitrogen analyzer is used for measuring; s3, nitrogen content determination: determining the nitrogen content in the treated sample by using an automatic nitrogen analyzer KjeltecTM8100; according to the method, high-throughput transcriptome sequencing and weighted gene co-expression network analysis (WGCNA) are combined, so that not only can gene expression maps of flue-cured tobacco leaves treated by different nitrogen fertilizers be comprehensively captured, but also gene modules with similar expression modes can be mined from a global perspective.

The invention provides an analysis method for high-throughput evaluation of tobacco nicotine synthesis capability, and belongs to the technical field of nicotine synthesis capability analys.The analysis method comprises the steps that tobacco seeds are disinfected, germinated and cultured for 14 days to obtain seedlings, the seedlings are transferred to a control medium and an induction medium added with 3 [mu] M of methyl jasmonate to be cultured for 15 days, and the seedlings are obtained; the method comprises the following steps: removing water from an overground part for 15 minutes at 105 DEG C, drying at 65 DEG C until the weight is constant, grinding the overground part into powder, adding a NaOH solution and ethyl acetate containing an internal standard, carrying out vortex mixing uniformly, carrying out ultrasonication for 15 minutes, taking supernate, filtering to obtain sample extract liquor, inputting peak area data into an intelligent prediction model based on a hierarchical weight mechanism, and processing to obtain prediction correction data, the nicotine concentration of a sample is calculated by combining gas chromatography detection with a standard curve, the concentration is corrected by utilizing predicted correction data, finally, nicotine content data is calculated according to the corrected concentration and the dry weight of the sample, and high-precision evaluation of the tobacco nicotine synthesis capability is realized.

The present disclosure relates to tobacco plants, plant parts, seeds, compositions and methods related to modulating expression of novel genes in tobacco to modulate alkaloid levels.

The present invention relates to a polypeptide CYS3 that affects seed germination. The CYS3 polypeptide molecule affects the germination of immature tobacco seeds. Immature tobacco seeds picked 20 days in advance after pollination are used as test materials. The CYS3 polypeptide is expressed and then treated in the tobacco seeds. It is found that the CYS3 protein has a germination-promoting effect on the immature tobacco seeds, providing a practical technology for promoting tobacco seed germination and seedling emergence in production.

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention discloses a short peptide PRP1 as well as a preparation method and application thereof. Belongs to the technical field of gene engineering. According to the application, an oligopeptide which is derived from unknown protein and can regulate and control the resistance of tobacco to the potato Y virus disease is obtained through research and identification, the oligopeptide is named as PVY residual peptide (PRP1), the amino acid sequence of the oligopeptide is ATKHIVGSIVAS, the oligopeptide PRP1 is prepared through a chemical synthesis method, and the oligopeptide is injected into the tobacco at the seedling stage, so that the PVY virus quantity can be effectively reduced, and the resistance of the tobacco to the potato Y virus disease can be improved.

The invention discloses a chelerythrine-containing compound microbial agent and application thereof, and belongs to the technical field of biological control. The invention provides a chelerythrine-containing compound microbial agent to solve the technical problems that in the prior art, only a single microbial agent and a plant extract are used, the biological control effect is poor, the stability is low, and the target spot is single. The compound microbial agent is prepared from a mortierella alpina extract, a trichoderma hairy extract and chelerythrine according to a specific compounding ratio. The compound microbial agent provided by the invention can delay the development of drug resistance of pathogenic bacteria through a multi-target effect, has better stability and stronger control effect, reduces the use amount of chemical pesticides, reduces the harm to the environment and human body, can be applied to tobacco planting production, can effectively reduce the control cost of tobacco black shank, and has broad application prospects. The economic benefit of tobacco planting is improved.

The present invention discloses a strain of Trichoderma wumengense GUWM22 and its application, which belong to the field of microorganisms. The deposit number of the Trichoderma wumengense GUWM22 is CCTCC NO: M20242639, the deposit date is November 26, 2024, the deposit unit is the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan, China. It has been verified that the GUWM22 bacteria have a strong inhibitory effect on 6 pathogens (Phytophthora nicotianae, Phytophthora capsici, Botrytis cinerea, Alternaria alternata, Phomopsis simiana and Echinops planifolia), and the inhibition rate is more than 78%. In addition, GUWM22 also has a good prevention and control effect on post-harvest anthracnose of pepper (strip pepper), with a prevention effect of 82.11%. It can be seen that Trichoderma wumengense GUWM22 is a new species of biocontrol Trichoderma with good potential. The present invention provides a new strain resource and an environmentally friendly and efficient biocontrol approach for the effective prevention and control of pepper anthracnose.

The invention relates to the technical field of agricultural microorganisms. The invention provides a compound microorganism bacterium suspension, a bacterium agent, a tobacco seed coating agent as well as a preparation method and application of the compound microorganism bacterium suspension. The compound microorganism bacterium suspension comprises a rice seed endophytic bacterium spiraceae R3 bacterium suspension and a rice seed endophytic bacterium stenotrophomonas R5 bacterium suspension; the mass ratio of R3 to R5 is 1: (0.5-1.5); the endophytic bacterium herbapiril R3 of the rice seeds is Herbapiril sp. R3, and the preservation number of the herbapiril R3 is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the rice seed endophytic bacterium stenotrophomonas R5 is Shenotrophomonas sp. R5, and the preservation number of the rice seed endophytic bacterium stenotrophomonas R5 is CCTCC (China Center for Type Culture Collection) NO: M 20231758. The compound microbial suspension is used as a seed coating agent to coat tobacco seeds, and growth of tobacco root systems can be remarkably promoted.

The invention relates to the technical field of tobacco production, and discloses a tobacco nitrogen fertilizer application method based on ion sensing, and the method comprises the following steps: soil foundation determination; applying a base fertilizer; transplanting and fertilizing; field topdressing; fertilizing in a vigorous growth period; management after fertilization: the nitrogen content of the soil is measured again 7-10 days after each time of topdressing, the fertilization frequency and dosage are adjusted according to monitoring data, stable nitrogen supply of the tobacco is ensured, and tobacco reviving or nitrogen fertilizer waste caused by excessive fertilization is avoided. By monitoring nitrogen ions in soil in real time, application of the nitrogen fertilizer is accurately regulated and controlled, the utilization efficiency of the fertilizer is improved, and tobacco growth is optimized. The precise fertilization method based on the ion selective electrode technology aims at solving the problems that in traditional fertilization, the nitrogen fertilizer dosage is difficult to precisely control, waste is caused, and tobacco leaves turn green. By monitoring the soil nitrogen ion concentration in real time and adjusting the fertilization amount according to the growth stage, the nitrogen fertilizer utilization efficiency is improved, the environmental pollution is reduced, the tobacco growth environment is optimized, and the tobacco yield and quality are improved.

The invention belongs to the field of microbial natural product pesticides, and particularly discloses a polyketone compound as well as a preparation method and application thereof. The compound disclosed by the invention is prepared from a fermentation crude extract of a strain of tobacco endophytic fungus Aspergillus japonicus TE-739D, and the compound disclosed by the invention is prepared from the fermentation crude extract of the tobacco endophytic fungus Aspergillus japonicus TE-739D. The chemical structure of the compound is identified through a modern spectroscopy technology, such as a high resolution mass spectrum HRESIMS and a superconducting nuclear magnetic resonance spectrum NMR, and the compound is named as japonidiene C. The invention further discloses a preparation method of the compound. The polyketone compound japondiene C has an obvious insecticidal activity on 2-instar larvae of spodoptera frugiperda, and can be used for preventing and treating the second instar larvae of the spodoptera frugiperda.

The invention discloses a panax notoginseng MYB transcription factor gene PnMYB8, the nucleotide sequence of the gene PnMYB8 is as shown in SEQ ID NO: 1, an MYB transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnMYB8 gene has the capability of improving fungal infection resistance of plants, the PnMYB8 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the panax notoginseng MYB transcription factor gene PnMYB8 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Cylindrocarpon destructor), the fusarium latericum (Fusarium latericum), the fusarium equiseti (Fusarium equiseti) and the phoma herbarum (Phoma herbarum) is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Fusarium latericum) is obviously enhanced.

The invention provides a microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening and a preparation method of the microbial agent type bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening comprises the following raw materials: an organic material, a microbial agent, an inorganic nutritional ingredient, vinasse and an additive, wherein the organic material is composed of livestock and poultry manure, crop straw, tobacco waste and a fermentation inoculant; the additive is prepared from humic acid, seaweed extract and amino acid. The organic fertilizer contains rich organic materials and humic acid, the content of organic matters in soil can be increased, the granular structure of the soil can be improved, the growth of soil-borne pathogenic bacteria can be inhibited by the added multiple beneficial microorganisms, the micro-ecological environment of the soil can be improved by the photosynthetic bacteria, and the incidence rate of diseases such as tobacco black shank and bacterial wilt can be reduced.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention relates to cloning of a stress-resistant gene LpbHLH75 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH75 of Lilium pumilum DC. The lilium tenuifolium stress resistance key genes are mined through transcriptome data analysis, and the functions of the lilium tenuifolium stress resistance key genes are studied. Comprising the following steps: (1) cloning a target gene; (2) constructing a plant overexpression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH75 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the function of overexpression of the LpbHLH75 on tobacco salt and alkali and drought stress is identified through transgenic tobacco, the function of the lilium tenuifolium bHLH gene under adversity stress is disclosed, a new gene source is provided for lilium tenuifolium genetic engineering breeding, a reference is provided for later lilium tenuifolium stress-resistant germplasm resource research, and the lilium tenuifolium bHLH gene is developed. And a foundation is laid for exploring a gene capable of enhancing stress resistance in the lilium tenuifolium and a stress resistance mechanism of the gene.