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2163 results about "Nicotiana tabacum" patented technology

Nicotiana tabacum, or cultivated tobacco, is an annually-grown herbaceous plant. It is found only in cultivation, where it is the most commonly grown of all plants in the genus Nicotiana, and its leaves are commercially grown in many countries to be processed into tobacco. It grows to heights between 1 and 2 meters. Research is ongoing into its ancestry among wild Nicotiana species, but it is believed to be a hybrid of Nicotiana sylvestris, Nicotiana tomentosiformis, and possibly Nicotiana otophora.

Application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation

InactiveCN103125251AImprove qualityImprove mineral nutritionHorticulture methodsBiotechnologySporeling
The invention relates to an application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary arbuscular mycorrhizal fungi fungicide is inoculated and propagated separately, and propagule spore density of an arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per gram of dry soil. A float breeding method is adopted to conduct arbuscular mycorrhizal fungi fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step inoculation, and the mixed fungicide is mixed by propagated acaulospora mellea, glomus mosseae and glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal fungus is close to a natural state, and fungus cooperate and play a better role of growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic system built during a seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.
Owner:SOUTHWEST UNIVERSITY

New technology for curing red flower gold dollar tobacco leaf to be yellow and fragrant

The invention discloses a new technology for curing the red flower gold dollar tobacco leaf to be yellow and fragrant, belonging to the technical field of tobacco preparation. The technology comprises the following steps of: a first stage: (1) a leaf changing period: drying a ball at 37-38 DEG C and wetting the ball at 35-36 DEG C till that the leaf is turned to be yellow at 7-8 level and the leaf is softened and withered, and (2) a withering period: drying the ball at 42 DEG C and wetting the ball at 37-38 DEG C till that the leaf is turned to be yellow at 9-10 level and a main vein is soften; a second stage: (1) a vein changing period: drying the ball at 45-46 DEG C and wetting the ball at 37-38 DEG C till that the yellow leaf and the yellow vein are changed to be the status of a small winding drum, and (2) a period for curing the dried leaf to be fragrant: drying the ball at 52 DEG C and wetting the ball at 39-40 DEG C for 12+/-0.5h till that the dried leaf is changed to be a winding drum; and a third stage: drying the ball at 65 DEG C and wetting the ball at 40-42 DEG C till that the vein of the full baked tobacco leaf is dried. The new curing technology can obviously reduce the green smoke rate and the curing phenomena of the green vein, the green leaf, the floating green and the like, and can improve the fragrant quality and the appearance quality of the tobacco leaf.
Owner:HUBEI CHINA TOBACCO IND

Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenic plastids

Transgenic chloroplast technology could provide a viable solution to the production of Insulin-like Growth Factor I (IGF-I), Human Serum Albumin (HSA), or interferons (IFN) because of hyper-expression capabilities, ability to fold and process eukaryotic proteins with disulfide bridges (thereby eliminating the need for expensive post-purification processing). Tobacco is an ideal choice because of its large biomass, ease of scale-up (million seeds per plant), genetic manipulation and impending need to explore alternate uses for this hazardous crop. Therefore, all three human proteins will be expressed as follows: a) Develop recombinant DNA vectors for enhanced expression via tobacco chloroplast genomes b) generate transgenic plants c) characterize transgenic expression of proteins or fusion proteins using molecular and biochemical methods d) large scale purification of therapeutic proteins from transgenic tobacco and comparison of current purification/processing methods in E. coli or yeast e) Characterization and comparison of therapeutic proteins (yield, purity, functionality) produced in yeast or E. coli with transgenic tobacco f) animal testing and pre-clinical trials for effectiveness of the therapeutic proteins. Mass production of affordable vaccines can be achieved by genetically engineering plants to produce recombinant proteins that are candidate vaccine antigens. The B subunits of Enteroxigenic E. coli (LTB) and cholera toxin of Vibrio cholerae (CTB) are examples of such antigens. When the native LTB gene was expressed via the tobacco nuclear genome, LTB accumulated at levels less than 0.01% of the total soluble leaf protein. Production of effective levels of LTB in plants, required extensive codon modification. Amplification of an unmodified CTB coding sequence in chloroplasts, up to 10,000 copies per cell, resulted in the accumulation of up to 4.1% of total soluble tobacco leaf protein as oligomers (about 410 fold higher expression levels than that of the unmodified LTB gene). PCR and Southern blot analyses confirmed stable integration of the CTB gene into the chloroplast genome. Western blot analysis showed that chloroplast synthesized CTB assembled into oligomers and was antigenically identical to purified native CTB. Also, GM1,-ganglioside binding assays confirmed that chloroplast synthesized CTB binds to the intestinal membrane receptor of cholera toxin, indicating correct folding and disulfide bond formation within the chloroplast. In contrast to stunted nuclear transgenic plants, chloroplast transgenic plants were morphologically indistinguishable from untransformed plants, when CTB was constitutively expressed. The introduced gene was stably inherited in the subsequent generation as confirmed by PCR and Southern blot analyses. Incrased production of an efficient transmucosal carrier molecule and delivery system, like CTB, in transgenic chloroplasts makes plant based oral vaccines and fusion proteins with CTB needing oral administration a much more practical approach.
Owner:AUBURN UNIV +1

Green biological seedling raising matrix

The invention discloses a green biological seedling raising matrix, relating to the seedling raising matrix technology. The preparation method comprises the following steps of: mixing straw powder, organic fertilizer and nutrient soil to be uniform, adding water until the water content is 45-55%, adding compound bacteria and then mixing to be uniform, stacking for fermenting, turning when the temperature increases to 50 +/- 2 DEG C, turning and cooling for 1-2 days when the temperature is increased to 50 DEG C again, adding microelements and growth hormone, stirring to be uniform, and packaging. The invention has the beneficial effects that: (1) the matrix is rich in organic matters and beneficial biological germs, has comprehensive nutrition and is loose and soft; (2) the matrix is seed-saving and labour-saving and can shorten seedling raising period; (3) the matrix can be used to ensure uniform and strong seedlings and high sprout rate; (4) the matrix can protect roots and hold water and fertilizer and has good air permeability; (5) the matrix has the functions of promoting flower bud differentiation and improving the yield; and (6) the matrix has the effect of resisting soil diseases. The matrix disclosed by the invention is applicable to seedling raising of vegetable, melon and fruit, flower and plant, tea, tobacco and cotton.
Owner:徐贵阁

Overwintering preserving breeding method for aphidius gifuensis

The invention relates to an overwintering preserving breeding method for an aphidius gifuensis and belongs to the technical field of biological control. The overwintering preserving breeding method for the aphidius gifuensis solves the problem that in the prior art, overwintering preservation is performed on the aphidius gifuensis difficultly, and the heavy demand for the aphidius gifuensis in a tobacco planting season in a coming year is difficult to meet. The overwintering preserving breeding method comprises a first step of greenhouse aphidius gifuensis selection which comprises cultivation of host plants, collecting of an aphidius gifuensis source, aphidius gifuensis inoculation in a greenhouse, and purification and rejuvenation; a second step of winter greenhouse aphidius gifuensis preservation which comprises cultivation of aphidius gifuensis preserving plants, aphidius gifuensis inoculation in the greenhouse, and purification and rejuvenation; and a third step of expanding propagation in the greenhouse. The aphidius gifuensis is successfully accumulated and preserved during the time from finish of massive reproduction of the aphidius gifuensis in August to April in a coming year and before massive reproduction of the aphidius gifuensis, and the aphidius gifuensises with enough quantity and good quality are provided for massive reproduction of the aphidius gifuensis in a next year.
Owner:YUXI TABACOO COMPANY OF YUNNAN PROVINCE

Microbial fertilizer special for tobaccos, and preparing method thereof

The invention provides a novel microbial fertilizer special for tobaccos. The fertilizer comprises the following components in parts by weight: 5 to 25 parts of a complex microbial inoculant and 75 to 95 parts of a carrier, wherein the complex microbial inoculant contains 1.6 to 8 hundred million cfu/g of bacillus subtilis, 1.6 to 8 hundred million cfu/g of bacillus licheniformis, 0.4 to 2.0 hundred million cfu/g of trichoderma harzianum and 0.4 to 2.0 hundred million cfu/g of calicheamicin streptomyces hygroscopicus; the carrier is a powdered material which are made by performing microbe fermentation and decomposition on rape seed cakes, bean pulp cakes and sesame-seed cakes, and then performing drying and sieveing. The microbial fertilizer contains bacterial strains for antagonizing fungus pathogenic bacteria, bacterial strains for preventing and curing the breeding of soil pathogenic bacteria, bacterial strains for improving the soil environment and the like. The carrier is fermented three-cake fertilizer. The invention also provides a preparing method for the microbial fertilizer. The microbial fertilizer special for the tobaccos, provided by the invention, is powder, is capable of absorbing the water and preserving the moisture. The fertilizer is mainly used as the base fertilizer when fertilizing, the soil fertility can be improved, the nutrient absorption of crops is improved, the diseases of a plurality of crops can be prevented, and the quality and the yield of tobacco leaves can be effectively improved.
Owner:HUNAN TAIGU BIOTECH

Tobacco active biological organic-inorganic special fertilizer and preparation method thereof

The invention discloses a tobacco active biological organic-inorganic special fertilizer and a preparation method thereof. The tobacco active biological organic-inorganic special fertilizer is prepared by the following steps of: selecting functional microbial strain and adding the following materials in percentage by weight: 50-70 percent of rapeseed meal, 20-40 percent of sesame seed meal and 10-20 percent of bean meal; mixing, then performing solid fermentation and performing enzymolysis on crude protein in cake meal to form a biological fermentation cake fertilizer with bioactive free amino acid and active small peptide; and then uniformly mixing with an activator and an inorganic fertilizer and chelating to prepare the tobacco active biological organic-inorganic special fertilizer. The tobacco active biological organic-inorganic special fertilizer is characterized by comprising the following raw materials in percentage by weight: 8-12 percent of activator, 20-30 percent of biological fermentation cake fertilizer and 55-75 percent of inorganic fertilizer. According to the invention, a method of combining an organic fertilizer with an inorganic fertilizer and combining nitrate nitrogen with ammonium nitrogen is adopted, the biological fermentation cake fertilizer with the rapeseed meal, sesame seed meal and bean meal as carriers is added, and the raw materials are mixed with the activator and the like to prepare a high-efficiency active fertilizer. The fertilizer has the characteristics of high nutrition, high activity and high efficiency; the tobacco plant root environment can be improved, the root system activity is enhanced, the leaf growth is promoted, the requirements of tobacco plant development on mineral nutrition are fully met, the yield of the tobacco plant and the quality of the tobacco leaf can be obviously improved, the environment pollution is reduced, and the soil is improved.
Owner:湖南金叶众望科技股份有限公司

Preparation method of composite extract for fermentation type tobacco

InactiveCN102226129ALess irritatingIncrease sweetness and fragranceTobacco preparationEssential-oils/perfumesMicroorganismYeast
The invention provides a preparation method of an essence for tobacco, particularly a preparation method composite extract for fermentation type tobacco, belonging to the technical field of tobacco. The preparation method comprises the following steps: (1) pretreating cherokee rose, wolfberry fruit and Tamarindus indica Linn, and preparing a culture medium; (2) inoculating: inoculating flavor-producing yeast into the culture medium; (3) fermenting; and (4) separating to obtain the composite extract. In the invention, cherokee rose, wolfberry fruit and Tamarindus indica Linn are used as raw materials to produce the composite extract for fermentation type tobacco with fruit fragrance by using microbial fermentation technology. When being adding into cigarettes, the composite extract for fermentation type tobacco can enhance the fragrance and sweetness in the mouth, soften the smoke, lower the irritation of cigarettes, improve the mouthfeel and reduce the residues. The microbial fermentation technology is utilized to produce the essence for tobacco, thereby obtaining a new economic, efficient and orientable way for producing essence for natural tobacco, and developing a novel essencefor tobacco, which can not be produced by the traditional essence production method.
Owner:HUBEI CHINA TOBACCO IND +1

Method for identifying resistance to tobacco bacterial wilt during seedling stage

The invention relates to a seedling stage identification method for the resistance of tobacco to the bacterial wilt. The seedling stage identification method comprises the steps of heat preservation pool construction, pool water heating and circulation, wilt bacterium inoculation, tobacco seedling, disease state investigation and grading. The method replaces a conventional field identification result with a seedling stage identification result, identifies a great amount of material in a smaller area, carries out the identification a plurality of times annually, improves the reliability of the result, shortens the identification cycle, saves the site and test cost, and covers little floor space. The seedling stage identification method for the resistance of tobacco to the bacterial wilt is characterized by rapid disease development, good result repeatability, and the like. The seedling stage identification method for the resistance of tobacco to the bacterial wilt can effectively remove the interference on the resistance result caused by soil temperature difference, strain pathogenicity difference, strain quantity difference and other diseases, thereby benefiting the recurrence and comparison of results from different years and different labs. The difference between the attack rates of infection resistant varieties and the disease indexes is obvious; and the appraisal result of the resistance of the variety is basically consistent with the perennial resistance appraisal result obtained by others. The method has greater application potential in the resistance identification and resistance heredity rule study of disease resistant resources and breeding medium materials.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI
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