12 results about "Axillary bud" patented technology

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

This invention relates to the field of gene regulation technology, specifically to the application of the RhATHB12 gene in regulating axillary bud germination in roses, along with its kit and regulation method. The nucleotide sequence of the RhATHB12 gene is shown in SEQ ID NO.1. Regulation involves silencing, knocking out, or inhibiting the expression of the RhATHB12 gene, which may promote axillary bud germination; overexpression of RhATHB12, which may inhibit axillary bud germination. This gene and its regulation method are of paramount importance for cut rose breeding and for improving the quality and efficiency of the industry.

The application discloses a method for constructing a Vietnamese terminalia regenerative system, and comprises the following steps: Vietnamese terminalia stem segment test materials are obtained by grafting seedling saplings with superior branches, semi-lignified stem segments with axillary buds are used as explants, and after disinfection, the explants are inoculated into an induction culture medium to induce axillary buds, then the induced axillary buds are subjected to micro-branch cutting to carry out proliferation and rooting culture, and a large number of Vietnamese terminalia regenerative plants can be obtained. The method has the advantages of high propagation efficiency, high phenotype consistency, good genetic stability and the like, greatly shortens the production cycle of Vietnamese terminalia seedlings, reduces the variation risk of the seedlings, and provides high-quality seedlings for ecological afforestation and landscaping.

The application belongs to the technical field of reed cultivation and relates to an efficient cultivation method for improving yield and quality of reed, specifically comprising the following steps: tissue culture of female reed stems with axillary buds, induction, multiplication, rooting to obtain tissue culture seedlings; transplanting in a seedling raising substrate containing a compound microbial agent, and spraying high-phosphorus nutrient solution and balanced nutrient solution in stages; before field transplanting, digging a planting ditch, dividing the dug soil into surface soil and deep soil, and mixing the surface soil and deep soil with organic fertilizer, inorganic fertilizer, wood ash and the like respectively to prepare bottom layer, middle layer and surface layer fertilizers, and applying the fertilizers into the ditch according to different depths; during the tillering period, applying high-nitrogen compound fertilizer by ditching, during the jointing period, applying high-potassium water-soluble fertilizer in combination with irrigation, and during the inflorescence formation to grain filling period, spraying silicon-potassium foliar fertilizer. The application significantly improves the germination rate, survival rate, biological yield and stem quality of reed through tissue culture, functional substrate seedling raising, three-dimensional layered fertilization and periodical precise water and fertilizer management.

This invention belongs to the field of plant tissue culture and rapid propagation technology. A method for establishing a rapid propagation system for *Symplocos buergeriana* includes inducing shoot growth using *Symplocos buergeriana* stem segments as explants, inducing and proliferating axillary buds on the stem segments, inducing callus tissue, inducing adventitious bud differentiation from callus tissue, and cultivating adventitious bud roots. The stem segment axillary bud induction and proliferation employ an integrated culture process, enabling directional induction of axillary bud germination and mass proliferation of clustered axillary buds without the need to change the specialized proliferation culture medium. Axillary bud differentiation and propagation are completed sequentially, rapidly obtaining a large number of robust clustered axillary buds. This invention's method for establishing a rapid propagation system for *Symplocos buergeriana* screens out an optimal culture medium formula and plant growth regulator ratio that is not limited by genotype differences and is universally applicable. It establishes for the first time a complete, stable, genotype-independent, highly versatile, and highly efficient tissue culture regeneration system for *Symplocos buergeriana*, providing reliable technical support for the protection of *Symplocos buergeriana* germplasm resources, large-scale rapid propagation of superior germplasm, and industrialized seedling production.

This invention discloses a suitable cultivation substrate for the growth of *Cymbidium goeringii* and its application, belonging to the field of plant cultivation technology. The cultivation substrate is composed of a single type of cedar sawdust and is used for *Cymbidium goeringii* cultivation. Using the cedar sawdust cultivation substrate, after 6 months of transplanting, the number of *Cymbidium goeringii* seedlings reached 2.20±0.45 seedlings / plant, higher than that of sphagnum moss substrate (1.20±0.45 seedlings / plant), coconut fiber substrate (1.80±0.84 seedlings / plant), and other tested substrates. This indicates that cedar sawdust can effectively promote the germination of axillary buds in *Cymbidium goeringii*, which is beneficial for the rapid propagation of seedlings.

This invention discloses a method for creating a long, fleshy-stemmed, multi-axillary-bud Chinese kale germplasm. The method comprises the following steps: A1) crossing kale with a purple, cylindrical, fleshy-stemmed Chinese kale to obtain the F1 generation; A2) self-pollinating the F1 generation plants to obtain the F2 generation; A3) selecting plants from the F2 generation with purple leaves or veins and continuously self-pollinating them to obtain homozygous lines, resulting in purple, long, fleshy-stemmed, multi-axillary-bud Chinese kale; or selecting plants from the F2 generation without purple leaves or veins and continuously self-pollinating them to obtain green, long, fleshy-stemmed, multi-axillary-bud Chinese kale. The Chinese kale germplasm created by this method exhibits excellent characteristics of multiple harvests and high yield, laying the foundation for the breeding of new varieties of long, fleshy-stemmed, multi-axillary-bud Chinese kale.

This invention discloses the application of the rose gene RhMYB1 in regulating axillary bud germination in roses. Relating to the field of molecular biology, this invention provides the application of products that inhibit the expression of the RhMYB1 gene or its transcribed and translated protein in regulating axillary bud germination in roses. The nucleotide sequence of the RhMYB1 gene is shown in SEQ ID NO.1. Based on the expression results of the RhMYB1 gene at different sites in rose buds, this invention found that its expression level is higher in upper buds (i.e., active buds). Transient silencing of the RhMYB1 gene in axillary buds showed that silencing the RhMYB1 gene significantly inhibited the germination and growth of axillary buds, while overexpression of RhMYB1 promoted the growth of axillary buds.

This disclosure provides a number of sequences involved in axillary bud growth in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences or transgenes of such sequences, and tobacco products made from tobacco leaf harvested from such plants.

This invention provides a method for rapid propagation of *Vernicia fordii* via tissue culture and its culture medium, belonging to the field of plant tissue culture technology. By optimizing explant sterilization, the active ingredients and concentrations in the primary induction medium, proliferation medium, and rooting medium, the culture conditions at each stage, and the substrate and temperature and humidity control for hardening and transplanting, this invention can significantly improve the survival rate, axillary bud germination rate, proliferation coefficient, rooting rate, and transplant survival rate of *Vernicia fordii* tissue culture.

This invention belongs to the field of plant genetic engineering technology, specifically relating to a method for inducing transgenic roots of Lycium barbarum mediated by Agrobacterium rhizogenes, comprising the following steps: S1, preparation of sterile explants of Lycium barbarum; S2, preparation of infecting bacterial solution; S3, pretreatment and negative pressure inoculation; S4, co-culture and gradient sterilization; S5, delayed culture and gradient resistance screening; S6, identification of positive hairy roots and root strengthening culture. This invention uses semi-lignified root-cutting stem segments with axillary buds as explants. The wound surface formed by the 45° oblique cut increases the infection site of Agrobacterium. Combined with negative pressure assisted inoculation technology, the transformation efficiency of Agrobacterium is significantly improved. Experimental verification shows that the induction rate of hairy roots of Lycium barbarum in this invention can reach more than 85%, and the positive transformation rate can reach more than 70%, which is far higher than the average level of 30-40% of the prior art.