1288 results about "Axillary bud" patented technology

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.

The present invention discloses a tobacco cultivation method. Said method is characterized by that it adopts a molecular biological technique to identify key gene capable of promoting axillary bud germination and growth after the tip is pruned, then uses nocuity inducible promoter to drive antisense RNA and make it promptly express after the tip is pruned so as to inhibit the expression of said key gene capable of promoting axillary bud germination and growth and make the axillary bud can not be germinated or grown.

The invention discloses a plant regeneration method for butterfly orchid, belonging to the field of butterfly orchid planting. The plant regeneration method comprises the following steps of: selecting pedicel axillary bud stems with length of 2-5 centimeters on butterfly orchid plants; carrying out induction culture on stem apexes for 2-3 weeks for gradually forming protocorms after 25-30 days; continuously splitting the protocorms to implement subculture acceleration reproduction; moving seedlings with height of one centimeter to a strong seedling culture medium to be cultured for 3-6 months; and inducing the seedlings to root for 20-25 days, and transplanting the strong seedlings with height of 8-10 centimeters, wherein the survive rate of the seedlings is higher than 95%, nursery-grown plants grow well after surviving. The plant regeneration method enables the excellent butterfly orchids to form sterile strains within a short time for implementing large-scale industrial production, can be produced yearly, has a high reproduction speed, and provides novel, fresh, fragrant and colorful flowers with various colors and consistent model. Moreover, a production technology with high technical content is provided to enterprises, so that the plants are consistent in size for facilitating control on flowering phases and uniform management of industrialization, the production costs of the enterprises are reduced, and giant economical benefits are created.

The invention relates to a rapid propagation method for tissue cultivation of a dendrobium candidum stem, belonging to the technical field of artificial cultivation of dendrobe. The method comprises the following steps of preparing an explant, inducing axillary bud and protocorm, conducting propagation cultivation, and rooting and seeding cultivation. The rapid propagation method has the beneficial effects that the technical problems of insufficient explant sterilization, high pollution rate, incapability of conducting axenic cultivation, slow protocorm differentiation, less differentiation, long propagation period, high production cost and the like can be well solved. Compared with the prior art, the rapid propagation method has the advantages that rapid propagation can be achieved; and the propagation period can be reduced by over 60 days. In addition, according to the rapid propagation method, seeding genetic traits are stable, the variation is small, the variety is pure, and the parent characteristics and features can be well kept. Thus, excellent seeding guarantee is provided for development of plant industry of dendrobium candidum, and the rapid propagation method has good economic benefit and social benefit.

The invention discloses a tissue culture rapid propagation method of liquorice. The axillary bud stems of the liquorice are taken as the explants to establish a liquorice in-vitro rapid propagation system, and a foundation is laid for the large-scale production of the liquorice. The stem with the axillary buds is taken as the explant, and the tissue culture rapid propagation of the liquorice is realized by use of the processes of multiple shoot induction, multiplication culture, root and shoot induction, test-tube plantlet acclimatization and transplant and the like; the technical support is provided for the industrialization development of the liquorice.

The invention relates to alpine rhododendron tissue culture quick-propagation method. All the medium is prepared according to following material: medium for inducing explant: 1/4MS, MS ferric salt, VitB50.2mg, lactalbumin hydrolysate 300mg, sucrose 30g+Kt1.0mg/L; medium for propagating cluster sprout: 1/2MS, Kt0.5mg/L; medium for rooting: 1/2MS, NAA5mg/L, active carbon 0.5%. The method of alpine rhododendron tissue culture quick-propagation is characterized as test tube sprout provisional planting method adopting tender stem segment axillary bud culture method and 'two-step' method. The advantages of the invention lie in: the growth is not influenced by season, plant has strong resistance to stress, and the plant has big colorful flowers.

The invention discloses a rapid propagation technique of Dendrobium candidum axillary buds. According to the technique, Dendrobium candidum axillary buds are used as explants directly to induce fasciculated buds; influences of combinations of different types of hormones with different concentration and different natural extracts on survival and growth of axillary buds, induction and multiplication of fasciculated buds and seedlings are compared, the quantity and quality of seedlings growing from fasciculated buds are researched and explored, the optimal culture medium formula in each stage is screened, and therefore, a fasciculated-bud-way high-efficiency rapid propagation system is established, so that plants obtained through rapid propagation are consistent with parent plants genetically so as to provide basis for restoration of wild population of Dendrobium candidum, and furthermore, wild resources are protected. Compared with vegetative propagation, the rapid propagation technique provided by the invention greatly improves propagation factor. A high-efficiency rapid propagation system of Dendrobium candidum which is an endangered plant provides a technical probability for the large-scale production and is beneficial to the restoration of wild population.

The invention relates to a tender stem callus plant regeneration induction culture medium for lonicera caerulea and solves the problems of low plant differentiation rate and high culture medium cost problem of the auxiliary bud or stem tip culture, which is the main tissue culture of lonicera caerulea. The culture medium consists of a large amount of macroelements, microelements and hormone, wherein the hormone consists of 6-benzyl aminopurine at a concentration of 1.0 to 2.0mg/L and indolebutyric acid or naphthylacetic acid at a concentration of 0.05 to 0.2mg. When the LD1 culture medium of the invention is used, the tender stems of lonicera caerulea can be easily induced to form calla, and the induction rates of the calla are all over 90 percent; and the probability of the induction of the calla into plants is high, the plant differentiation rate is up to 50 to 82 percent which is 66.6 to 173.3 percent higher than that of the conventional Murashige-Skoog (MS) culture medium, and the cost is saved by 45.1 percent compared with the reference MS culture medium.

The invention provides a method of rapid breeding of kiwi fruit with large seed by tissue culture. The invention comprises steps of selecting and treating explant, inducing culture medium, root culturing and replanting, etc. The invention takes MS culture medium as basis and adds BA, NAA, GA, ZT and other external plant growth regulator to induce the growth of axillary bud of the said kiwi fruit and strengthen sprout and root. The root growth rate is as high as 100%, the average bud height is 6.28 cm and the average replanting survival rate is 91% after 35 days culture. The invention solves the technique problem of rapid breeding of said kiwi fruit and provides a method of short period, high breeding rate and low cost for the industrial production of kiwi fruit with large seed.

The invention relates to a method for rapid propagation and timely flowering of China rose, and relates to the technical field of flower planting. The method comprises the following steps: in January and February, scissoring annual stingless wild rose branches, and reserving one or two bud points to be taken as stocks for grafting; selecting annual China rose branches as scions, reserving one or two bud joints, cutting the upper ends of the scions to be flat, cutting the lower ends of the scions to be inclined planes with the angles of 30-45 degrees, wherein the distances between pruning wounds and axillary buds are 1 cm; putting the cut stocks and scions in nutrient solution and soaking for 10-20 minutes, then grafting the soaked stocks with the scions together, tightening grafting ports with plastic films, and then daubing rooting powder on the roots of the stocks and inserting the roots of the stocks in soil; beginning to transplant two months later after the stocks are alive through cottage, irrigating and fertilizing according to a conventional management method 15-20 days later, wherein the China rose can be flowered in May. The method is simple and feasible; compared with a traditional grafting method, the survival rate can reach more than 90%, nursery stocks grow strongly, the China rose can be flowered timely, the planting cost is reduced, and the economic benefit is improved.

The invention discloses a modified medium for improving propagation of stems of Dendrobium officinale and a propagation method. The medium comprises MS (Murashige and Skoog) basal medium, cane sugar 20g/l, agar 6g/L, 6-BA (benayl aminopurine) 2mg/l, NAA (alpha-naphthaleneacetic acid) 0.2-0.5mg/l, banana mash 15-20g/l, potato mash 15-20g/l, and common tap water replacing distilled water. The modified MS medium is used to propagate the stems with axillary buds of Dendrobium officinale, alkaloid and polysaccharide content of Dendrobium officinale is evidently increased, and Dendrobium officinale grow normally. By the method, consumption of cane sugar is reduced, the tap water is used to replace the distilled water, and accordingly tissue culture technology for Dendrobium officinale is more convenient, more efficient and resource-saving.

The invention relates to a tissue culture propagating method of Manihot esculenta Crantz. As one of the global three-top tuber crops and the number one alimentarn crop in Africa, the Manihot esculenta Crantz is currently ranked as a vital biological energy crop in our country. Stem section vegetative cottage is commonly adopted for propagation, and the low propagation rate thereof is a restrictive factor for popularizing the new variety and shortening the breeding period. The method of the invention comprises the following steps: the Manihot esculenta Crantz stem section with stem apexes or lateral buds is taken as an explant, and the Manihot esculenta Crantz tissue culture plantlets are quickly obtained in virtue of the micro-propagation method; young leaves, stem apexes and axillary buds of the sterile plantlets obtained from micro-propagation are taken as explants, somatic embryo generation and plant regeneration are induced, thus the Manihot esculenta Crantz somatic cell regeneration system is established. By adopting the method, the Manihot esculenta Crantz has the advantages of high propagation rate, no genotype dependence, and the like; the method has high value on the quick propagation and large-scale production of the Manihot esculenta Crantz improved variety in a short term, and establishes a technical basis for the Manihot esculenta Crantz transgene and other breeding.

The invention relates to a vegetative propagation method for cattleya, in particular to a vegetative propagation method for butterfly orchid. The vegetative propagation method for butterfly orchid, provided by the invention, comprises the following steps of: by taking pedicel and axillary bud of butterfly orchid as explant, inoculating the explant into a culture medium for culturing; and performing subculture, rooting culture and transplanting culture on the induced new buds in turn. According to the method provided by the invention, the tillering of the butterfly orchid is realized in an artificially prepared culture medium; the single-stalk reproduction feature of the butterfly orchid is broken through; an induced pedicel seedling can be obtained without hurting a butterfly orchid stock plant according to the method; and the pedicel of the same stock plant can be repeatedly used. The seedlings produced according to the method are robust, trim and consistent; the source problem of excellent butterfly orchid seedlings during production is solved; and more economic and social benefits can be created.

The invention provides a method for quickly planting cynodon dactylon on an ecological concrete slope. The method comprises the following steps of 1 slope surface preparing; 2 lattice frame constructing, wherein multiple lattice frames adjacent to one another are poured and are all backfilled with planting matrixes, the planting matrixes are leveled, tamped and covered with first non-woven fabric; 3 ecological concrete pouring; 4 cynodon dactylon planting, wherein the stolons of cynodon dactylon are sheared into cynodon dactylon stems; 5 curing. According to the method, the cynodon dactylon which is good in stress resistance, resistant to trampling and poor soil and high in recovery capacity is adopted to serve as the planting species, the survival rate is high, and the selected cynodon dactylon is at least two years old; after twenty days, the coverage rate of the cynodon dactylon can reach 80 percent, the cynodon dactylon stems grow well, adventitious roots are grown, axillary buds on the stems begin to grow into branches on the ground, and new stolons begin to tiller; after thirty days, the vegetation coverage rate is more than 95 percent. By adopting the method, vegetation landscapes can be quickly formed, the slope ecology environment can be improved, and slope ecology protection can be achieved.

The invention belongs to the field of plant tissue cultivation, and particularly relates to a method of cutting propagation of a peony immature stem. The method includes the steps of material selection, cutting cultivation, indoor management, oversummer maintenance, overwinter management and strong seedling cultivation. By using low-frequency ultrasonic waves to conduct processing, rooting can be promoted, plant cell division can be remarkably accelerated and induced, the cell growth is stimulated, protein synthesis of protoplast can be accelerated, and the adventitious bud reproducibility can be improved. Due to the fact that intermittent irradiation is adopted, no drastic cavitation effect can be produced, and partial damage to cells caused by the ultrasonic waves can be avoided. Due to the fact that cultivation and rooting are conducted in the dark cultivation process, growth inhibitor is greatly reduced, and rooting is facilitated. The complete and specific method including the steps of oversummer maintenance, auxiliary bud inducing, overwinter management and strong seedling cultication is provided, and therefore application of the peony rapid cutting propagation technology in actual production is promoted, and the method has significance in large-scale production of peony seedlings.

The invention relates to a forest tree seedling breeding technology and in particular relates to a method for suppressing browning of a carya cathayensis sarg explant. The method is characterized by selecting a carya cathayensis sarg stem with axillary buds as an explant in spring, disinfecting the explant, then inoculating the explant into a culture medium to which catechins and anti-browning agents are added, and firstly culturing a tissue culture vessel in the low temperature dark environment and then transferring the tissue culture vessel to the routine culture environment at the initial stage of inoculation. The method can conduce to obvious reduction of the browning rate of the carya cathayensis sarg explant and improvement of inductivity of the carya cathayensis sarg explant, is simple and convenient to operate, has a better browning suppression effect, and provides a technical support for tissue culture and asexual reproduction of carya cathayensis sarg.

The invention belongs to the technical field of plant tissue culture and particularly relates to a method for building a bamboo reed tissue culture system. The method comprises the steps of (1) explant disinfection, wherein axillary buds of bamboo reeds are fully soaked in 75% of ethanol for 10 seconds and then moved into 0.2% of mercury bichloride solution for 10 minutes; (2) primary culture, wherein a culture medium is formed by adding cane sugar 30g/L and agar 8g/L in MS, then adding 6-BA 5mg/L and IBA 0.5mg/L and finally adjusting potential of hydrogen (pH) to 5.8; (3) secondary culture, wherein a culture medium and culture conditions of the secondary culture are the same as the culture medium and culture conditions for the primary culture; (4) strong bud and root culture, wherein the buds are cultured in the secondary culture medium for one month to finish strong bud culture and then planted in a rooting culture medium, and the rooting culture medium is formed by adding IBA 0.5 mg/L into the MS; and (5) domestication and transplantation, wherein the domestication is performed by using a floating bed domestication system for one week and then transplantation is performed. By means of the method, the efficient breeding of the bamboo reeds can be achieved.

The invention discloses a method for grafting of a cucumber on a black-seed pumpkin in Yunnan, belonging to the field of crop breeding. According to the method, the black-seed pumpkin in Yunnan is used as a stock, a growing point and axillary buds on two sides of the stock are removed by a blade, a special bamboo stick of 0.5cm is vertically inserted into the center of the growing point and is maintained in a stem, a cucumber seedling is taken, the under-cotyledon part is removed by obliquely cutting at 0.4-0.6cm under the cotyledon of the cucumber seedling for an angle of 25-30 degrees, the bamboo stick is taken out, the cucumber seedling is inserted into a grafting hole, and the cotyledon of the cucumber and the cotyledon of the stock are arranged in a cross-shaped vertical shape; and after grafting is performed, moisture is kept for 3-5 days in an enclosed environment in a greenhouse, the temperature is maintained at 25-30 DEG C at daytime and 18-20 DEG C at night for seedling hardening, then ventilation is performed gradually, and complete ventilation is performed on the ninth or tenth day. The black-seed pumpkin in Yunnan is used as the stock in the method, diseases which can easily occur to certain cucumber seedlings can be eliminated completely, and the stress tolerance and disease resistance can be better improved. Since direct insertion is adopted for grafting, the rate of incidence of adventive roots is very low, and the survival rate of grafted seedlings can be guaranteed to be up to 98 percent.

The invention provides a pruning, reshaping and yield-increasing method of kiwi fruit in a mountain land. The method comprises the following steps: shearing the tip of the main stem of the kiwi fruit so that two buds at the upper end are cultured to form main branches and tendrils which respectively grow along the two sides of a shed frame; shearing and removing unnecessary main branches and tendrils and binding the branches and tendrils in parallel at the end of the year; in the second year, leaving axillary buds at the even number positions of the main branches and tendrils to be used as fruiting mother branches in the same year, and eliminating the axillary buds at the odd number positions of the main branches and tendrils to be used as the fruiting mother branches in the next year; and after fruiting on the fruiting mother branches and harvesting, pruning the fruiting mother branches in the end of the same year so that new buds can grow in the next year and become growing branches, in such a way, alternatively culturing and pruning the axillary buds at the even and odd number positions and the fruiting mother branches, and culturing and pruning repeatedly and circularly annually. The method provided by the invention has the beneficial effects that unnecessary branches are sheared and removed annually and abstemiously according to different positions and a planned way, the unnecessary axillary buds are eliminated, fruiting branches after harvesting are sheared and removed, the fruiting mother branches and the growing branches alternatively develop and mature annually, and nutrition substances in soil are concentrated to mainly be supplied to fruits, so that the yield per mu is improved.

The present invention discloses tissue culture process of alternate leaf cajeputtree as one kind of perfume plant. The tissue culture process includes the following steps: cutting axillary bud as the explant from the mother body, washing and sterilizing; the subsequent inducing culturing in inducing culture medium comprising 7/10MS-MS, 6-BA 0.1-0.3 mg/L and NAA 0.1-0.3 mg/L; secondary culturing in secondary culture medium comprising 7/10 MS-MS, 6-BA 0.4-0.6 mg/L and NAA 0.05-0.15 mg/L; and final rooting culturing in rooting culture medium comprising 2/5MS -3/5MS, and IBA 0.5-0.7 mg/L. The tissue culture process has the culturing conditions including illumination intensity of 2500-3000Lux, and temperature of 26+/-1 deg.c. The tissue culture process is suitable for fast propagation in industrial scale.

The invention discloses an asexual rapid propagation method for radix glycyrrhizae. The asexual rapid propagation method comprises the following steps: inoculating an explant of a radix glycyrrhizae stem section into a culture medium for cultivation to obtain an aseptic seedling; then cutting the aseptic seedling into a terminal bud explant, a stem section explant with one axillary bud and an explant with one axillary bud and a root, inoculating the explants into the same culture medium for cultivation to obtain a subculture-multiplicative aseptic seedling; and transplanting the subculture-multiplicative aseptic seedling into the culture medium to realize asexual rapid propagation for the radix glycyrrhizae. The formula of the used culture medium is that IBA with the concentration of 0.1mg/L is added to the MS culture medium, and the using amounts of KNO3 and NH4NO3 in the MS culture medium are halved respectively, and the pH value of the MS culture medium is 5.8. According to the asexual rapid propagation method, the root-inducing rate of the aseptic seedling is 100%, so that seedling emergence can be realized after 40 days, the seedling can be transplanted into a large field after 50 days, and the survival rate in the large field is higher than 80%. The asexual rapid propagation method can be suitable for rapid propagation of a plurality of excellent strain systems of a plurality of radix glycyrrhizae varieties, and has remarkable ecological, economic and social benefits in sustainable development of the radix glycyrrhizae.

The invention provides a method for tissue culturing and reproducing the millettia speciosa champ germchit. By using the tissue culture method and taking the fresh apical bud and the axillary bud of the wild millettia speciosa champ as the explant, the explant is disinfected and sterilized by the 75 percent alcohol and 0.1 percent mercuric chloride solution; after that, according to different periods, different culture medium formulas are adopted respectively for the bud induction culture, bud subculture proliferation and the rooting culture to obtain the container seedling of the millettia speciosa champ, wherein, the bud induction culture medium has a formula of MS+6-BA0.4mg.L-1+IBA0.2mg.L-1+3 percent white granulated sugar+0.35 percent agar, the formula of the bud subculture proliferation culture medium is MS+6-BA0.6mg.L-1+NAA0.5mg.L-1+3 percent white granulated sugar+0.35 percent agar, and the formula of the rooting culture medium is 1/2MS+ABT10.8mg.L-1+IBA0.4mg.L-1+3 percent white granulated sugar+0.35 percent agar; moreover, when the height of the container seedling is grown about 2 cm, the container seedling is again transplanted to a seedbed of a greenhouse for culturing; after reaching over 20cm, the container seedling is the grown seedling of the millettia speciosa champ germchit.

The invention relates to a tobacco field management method for improving quality of upper tobacco leaves. By the adoption of a cultivation method that the tops of tobacco are sleeved with plastic bags instead of topping, from the first blooming stage to the full blooming stage of the tobacco, all inflorescences together with three to five small tobacco leaves at the topmost portion are sleeved with shading materials instead of traditional manual topping, and therefore flowers and the leaves at the tops of the tobacco gradually die under the high-temperature, high-humidity and dark environment in the bags. Compared with the prior art, the tobacco field management method has the advantages that the apical dominance of the tobacco fades away, nutrients of the tobacco and coordination of chemical components of the tobacco leaves are guaranteed, and the effect of improving the quality of the upper tobacco leaves is obvious; the apical dominance of tobacco plants fades away, growth and development of axillary buds are restrained, the use quantity of suckercides is reduced, and quality safety of the tobacco leaves is improved; if bud picking is carried out manually, the frequency of bud picking is reduced, and labor is relieved; the bags are made of blue or black plastic materials convenient to obtain, cost is low, the bags are cleared away together with tobacco stems later, and no environment pollution is caused.

The invention discloses a non-tube method for fast breeding aleurite seedling, comprising: taking disinfected stem carrying axillary bud of 2-3cm length as explant; enclosing its morphological upper notch, immersing its morphological lower notch in indolebutyric acid solution, cutting it in sand disk, dressing nutritional solution to keep sand disk wet, culturing it for 16. 2-20. 2 days under conditions of 25-28 Deg. C, 70-80% of relative humidity, 2000-3000 lx lighting strength and light period of 12 hour lighting/ 12 hour dark, and getting rooting and integral plant. The invention is characterized by fast breeding, free from influence of national factors such as season and region, low variation rate, simple process and low cost.

The invention relates to a method for quickly breeding an Akebia trifoliata Koidz fruit seedling in a test tube, comprising the following steps of: inducing Akebia trifoliata Koidz to generate cluster buds, placing the cluster buds into a culture medium for rooting and culturing; then culturing a plant to the height of about 7cm at the temperature of 25 +/- 2 DEG C in an illumination environment; taking out the cluster buds from the culture medium; cutting the seedling in the test tube with a scalpel into stem sections with axillary buds of about 1cm for rooting and culturing; and then illuminating the plant to grow into about 7cm again, i.e. illuminating again and transplanting the plant to a cultivation substrate for growing. By adopting a genetic engineering technology of plant cells, the method can greatly shorten the breeding time, quicken the breeding progress and obtain a new species with more ideal characteristics purposefully.