134 results about "Axillary bud" patented technology

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The invention discloses a tissue culture method of a citral type cinnamomum bodinieri stem with an axillary bud, and relates to the technical field of forest tree tissue culture. The tissue culture method comprises the following steps: selecting a current-year semi-lignified stem on a citral type cinnamomum bodinieri excellent single plant for disinfection treatment, and inoculating the stem on an axillary bud primary induction culture medium for axillary bud primary induction culture; after stem axillary buds in the axillary bud primary induction culture medium germinate, stem segments with axillary buds are soaked with a phycocyanobilin solution and then inoculated into the adventitious bud induction culture medium, red light irradiation treatment is conducted, conventional adventitious bud induction culture is conducted after red light irradiation, adventitious bud subculture multiplication culture, strong seedling culture and rooting induction culture are conducted, and the stem segments with the axillary buds are obtained. And finally, hardening seedlings and transplanting. According to the tissue culture method provided by the invention, the adventitious bud multiplication coefficient can reach more than 5, the rooting rate reaches up to 90.00%, the average rooting number also reaches 3.44, and the survival rate after two months is more than 85%. The invention provides support for better development and utilization of citral type Cinnamomum bodinieri, and has important significance for popularization and application of citral type Cinnamomum bodinieri.

The invention relates to the technical field of agricultural breeding, in particular to a bamboo reed saline-alkaline resistant screening and stress-resistant tissue culture seedling raising method which comprises the following steps: step 1, pretreatment; step 2, dynamic salt stress screening: placing the seed stems in a mixed salt-alkali solution with gradient increasing, culturing, measuring the bud length and root length growth rate of axillary buds of the seed stems, fitting a salt concentration-growth rate curve, and extracting a semi-inhibitory concentration IC50 and a maximum tolerance concentration MTC; step 3, salt tolerance grade classification; 4, carrying out stress-resistant tissue culture seedling culture: carrying out in-vitro culture on the axillary bud explant of the high-salt-resistant variety in a culture medium, and inducing clumpy buds and rooting in combination with a salt-resistant inducer; 5, domestication and transplanting: performing gradual salt stress domestication on the tissue culture seedlings, transplanting the tissue culture seedlings into a saline-alkaline environment, and monitoring the adaptability of the tissue culture seedlings in the saline-alkaline environment. According to the method disclosed by the invention, the efficient cultivation system of the suitable-to-grow bamboo reed seedlings in the saline-alkali soil is established by fusing dynamic stress response analysis and stress resistance phenotype directional strengthening technologies.

The invention discloses a dendrobium officinale tissue culture and rapid propagation seedling raising method, which belongs to the field of biotechnology, and comprises the following steps: S1, explant treatment: selecting a dendrobium officinale plant with good growth vigor and no obvious pest and disease damage, carrying out high temperature stress treatment at 40-45 DEG C for 6-8 days, then taking the tender stem of the dendrobium officinale plant subjected to high temperature stress treatment as the explant, and carrying out tissue culture and rapid propagation on the explant; directly transferring the explant to a low temperature of 2-6 DEG C for stress treatment for 8-12 hours; s2, callus induction: taking the explant treated in the step S1, disinfecting the explant, taking the explant without axillary buds, cutting the explant into small blocks, inoculating the small blocks to a callus induction culture medium, and carrying out dark culture for 12-15 days; the callus induction culture medium comprises 1.5 to 3 mg / L of MS + 2, 4-D, 2 to 3 mg / L of KT and 0.1 to 0.3 mg / L of NAA; s3, bud differentiation induction and rooting induction. According to the tissue culture and rapid propagation method for the dendrobium officinale, the browning rate of the callus during callus induction can be effectively reduced, and the tissue culture seedling raising efficiency is improved.

The invention discloses application of an OsDrAp1 gene in regulation and control of ratooning rice axillary bud germination, and belongs to the technical field of biology. The nucleotide sequence of the OsDrAp1 gene is shown as SEQ ID NO.1. According to the invention, by observing the regeneration bud germination of a knockout and overexpression material of the OsDrAp1, the germination rate of a knockout plant is obviously improved, and the germination rate of the overexpression material is obviously reduced, so that the OsDrAp1 is proved to negatively regulate the regeneration rice axillary bud germination, and the OsDrAp1 can be applied to the breeding research of the regeneration rice.

The invention discloses an artemisia apiacea tissue culture and one-step seedling forming method, which comprises the following steps: cutting stems with axillary buds of artemisia apiacea as explants, and carrying out sterilization and inoculation pretreatment; inoculating the explants on an induction culture medium for culturing, and inducing the explants to generate cluster buds; transferring the germinated cluster buds to a proliferation culture medium for proliferation culture of the cluster buds; and cutting the proliferated non-rooted seedlings, and transferring to a rooting culture medium for rooting culture. According to the method, single high-concentration excitation 6-BA is used as a hormone for inducing the culture medium, axillary bud stem explants can be directly induced to generate cluster buds, and the generation of calluses is remarkably reduced. Meanwhile, in the subculture multiplication culture, after the subculture stage and the concentration of the used 6-BA are flexibly adjusted, the generation of calluses and the generation of vitrification phenomena can be further reduced. According to the method, the cluster buds can be rapidly rooted, so that a large number of seedlings are obtained, and the method has the remarkable advantages of saving time and manpower and material resource cost in production.

The invention discloses a tomato axillary bud real-time identification, measurement and removal point positioning method. The method comprises the following steps: acquiring RGB and depth images of a tomato plant by using a mobile phone or RGB-D sensor equipment; a lightweight model is adopted to carry out instance segmentation on the RGB image, and axillary buds and branches are identified and segmented. And then, aligning the binary mask of the axillary bud with the depth image, constructing corresponding three-dimensional point cloud data, and calculating the length of the axillary bud and judging the removability based on whether the point cloud and the axillary bud region contain axillary bud bifurcation. And after determining that the axillary buds can be removed, further determining axillary bud removal points. By means of the method, agricultural management equipment can rapidly, automatically and accurately obtain the axillary bud length and judge the removability of the axillary bud, and therefore tomato pruning operation is efficiently completed.

This invention discloses a method for rapid seedling cultivation of Eucommia ulmoides via tissue culture, relating to the field of Eucommia ulmoides breeding technology. The method includes the following steps: using young, tender stem segments with axillary buds from superior Eucommia ulmoides strains as explants, after surface disinfection, the segments are cut into sections with axillary buds and inoculated into an axillary bud induction medium to induce axillary bud germination; the germinated axillary buds are cut into segments and inoculated into a specific medium for adventitious bud induction and elongation culture; the adventitious buds after proliferation culture are separated and subjected to a two-step in-bottle rooting method or external adventitious root induction, ultimately obtaining complete regenerated Eucommia ulmoides plants. Beneficial effects: This invention develops a simplified Eucommia ulmoides tissue culture propagation method that shortens the production cycle, has a high propagation coefficient, rapid rooting, high adventitious root induction rate, and minimal basal callus. It can rapidly obtain high-quality Eucommia ulmoides seedlings, which is of great significance for the large-scale propagation of superior strains and varietal improvement research, while also providing technical support for the sustainable development of the Eucommia ulmoides industry.

The invention discloses a method for in-vitro preservation of common wild rice and Nwa wild rice, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a stem node with an axillary bud as an explant; soaking in 75% alcohol for 10-15 seconds, soaking in 10% 84 disinfectant for 15 minutes, performing vacuum treatment in 0.1% mercuric chloride solution under-0.1 Mpa for 5 minutes, and performing water bath at 40 DEG C for 20 minutes for combined sterilization; inserting the sterilized explant into a bud differentiation culture medium containing 2mg / L KT and 0.1 mg / L NAA, and culturing for 3-5 days under the conditions that the temperature is 23 DEG C and illumination is performed for 16 hours per day; cutting off buds growing to 3-5cm, and transferring the buds into a rooting culture medium; (5) carrying out test-tube plantlet propagation through stem node shearing or tillering cutting; the tissue culture seedlings with well developed root systems are hardened and then transplanted to a mixture of vermiculite and nutrient soil (v / v = 1 / 1). According to the method, the pollution rate is as low as 16.35%, the differentiation rate of the explant reaches 47.36%, the process is simple, convenient and efficient, the production period is short, the seed quality resources of common wild rice and Nilval wild rice can be effectively preserved, and the method has important significance on protection of precious wild rice resources.

The invention relates to the technical field of seedling raising, and discloses an efficient tissue culture and rapid propagation seedling raising method for xanthoceras sorbifolia bunge, which comprises the following steps: selecting and pretreating explants, selecting current-year robust branches of the xanthoceras sorbifolia bunge, cutting stem segments with axillary buds as the explants, cleaning, trimming and disinfecting the explants, performing surface disinfection on the pretreated explants, and performing tissue culture and rapid propagation seedling raising on the pretreated explants to obtain the xanthoceras sorbifolia bunge. When the Xanthoceras sorbifolia Bunge is subjected to efficient tissue culture and rapid propagation seedling culture, an operation standard considering thorough sterilization and tissue activity protection is established by standardizing an explant selection standard and executing a step-by-step surface disinfection procedure, so that the microbial contamination rate caused by incomplete disinfection of the explant can be reduced, meanwhile, tissue activity damage caused by excessive disinfection is reduced, and the survival rate of the Xanthoceras sorbifolia Bunge is improved. Therefore, sufficient and healthy sterile initial materials are guaranteed to be obtained, the problems of high primary culture pollution rate and induction starting failure are solved, and a reliable foundation is laid for establishment of a whole tissue culture and rapid propagation system.

The invention relates to the technical field of plant genetic engineering, and discloses a strawberry stolon regulation gene ZFP6 and application thereof, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. Researches prove that the ZFP6 gene is specifically expressed in axillary meristem of strawberry in the adult-plant stage, and the GA20ox4 gene can be directly or indirectly activated to start a biosynthesis pathway of GA and drive axillary buds to develop towards stolons. Through genetic interaction analysis of the ZFP6 gene, the GA20ox4 gene and the RGA1 gene, the function of the ZFP6 gene as an axillary bud fate decision upstream regulation factor is further verified, the blank of an endogenous regulation mechanism of the strawberry stolons is filled, and the ZFP6 gene is proved to be a molecular target for specifically regulating formation of the strawberry stolons. The gene sequence, the primer, the gene knockout expression vector and the matched technical system provided by the invention lay a foundation for strawberry molecular breeding and industrial application.

The present application relates to asparagus tissue culture technical field, specifically to a kind of asparagus tissue culture rapid propagation seedling cultivation method.A kind of asparagus tissue culture rapid propagation seedling cultivation method, comprising: the disinfection and browning prevention treatment of explant, preparation of composite antibacterial agent, preparation of culture medium, asparagus explant culture and transplanting.The present application adopts ozone fumigation to combine ultraviolet light disinfection, synergistic sterilization, covers surface and deep layer, reduces explant injury and browning.Browning prevention agent inhibits polyphenol oxidase, removes free radicals, reduces browning, promotes axillary bud germination.Add composite antibacterial agent in induction culture medium, destroys microbial cell membrane, has antibacterial and antioxidant effects, and guarantees explant growth.Compound rooting agent contains activated carbon, chitosan and sucrose, adsorbs harmful substances, stimulates root differentiation and provides energy, improves rooting rate and plant robustness.Overall, it improves the success rate of asparagus tissue culture.

This invention relates to the field of gene regulation technology, specifically to the application of the RhATHB12 gene in regulating axillary bud germination in roses, along with its kit and regulation method. The nucleotide sequence of the RhATHB12 gene is shown in SEQ ID NO.1. Regulation involves silencing, knocking out, or inhibiting the expression of the RhATHB12 gene, which may promote axillary bud germination; overexpression of RhATHB12, which may inhibit axillary bud germination. This gene and its regulation method are of paramount importance for cut rose breeding and for improving the quality and efficiency of the industry.

The invention discloses a phantom liquidambar formosana axillary bud tissue culture proliferation method, which takes phantom liquidambar formosana axillary buds as tissue culture materials and improves the propagation speed of phantom liquidambar formosana seedlings. After axillary buds of the 'phantom' liquidambar formosana hance are collected and treated, the most suitable culture medium and growth regulator are screened through primary culture, subculture and rooting culture, so that tissue culture proliferation of the 'phantom' liquidambar formosana hance is completed. According to the method, the population cardinal number of the phantom liquidambar formosana hance can be rapidly increased, the problem that the grafting speed is not high due to the fact that branches of the phantom liquidambar formosana hance are thickened slowly is solved, and a clonal germplasm resource nursery of the phantom liquidambar formosana hance can be built early.

The present invention relates to the technical field of fruit industry, and specifically discloses a regulation method and application for promoting and strengthening the flower formation of citrus. The regulation method is to spray flower-promoting regulator A during the ripening stage of autumn shoots of citrus; spray flower-promoting regulator B from 1 week before the appearance of flower buds in the following year to the flower bud stage of citrus. The components and spraying concentration of the flower-promoting regulator A are: S-abscisic acid 15-50 μg / ml, prohexadione-calcium 25-100 μg / ml, chlorophyllin iron 0.05-1 μg / ml, and the balance is an agriculturally acceptable adjuvant; the components and spraying concentration of the flower-promoting regulator B are: S-abscisic acid 1-35 μg / ml, 6-benzylaminopurine 10-70 μg / ml, and chlorophyllin iron 0.1-2 μg / ml, and the balance is an agriculturally acceptable adjuvant. The present invention can regulate the balance of flower-forming hormones in axillary buds of the tree body, effectively increase the flower formation amount of citrus in the following year, improve the quality of flower buds and the flowering uniformity, reduce the proportion of deformed flowers, reduce the difficulty of fruit retention, increase the citrus yield and improve the quality.

The invention provides an idesia grafting seedling raising method, relates to the technical field of grafting seedling raising, and adopts the technical scheme that the method comprises the steps of scion type determination, grafting method determination, grafting time determination and the like. According to the statistical data of the survival rate, the determination of appearance growth indexes of the grafted seedlings, and the fruit yield and quality, the optimal grafting mode is determined to be grafting in an axillary bud cut-grafting mode at the beginning of spring. The grafting combination most suitable for idesia polycarpa branch grafting is researched from different grafting periods and different grafting methods, a set of grafting method suitable for idesia polycarpa branches is established, and a set of feasible grafting method capable of achieving industrialized seedling raising is provided for asexual propagation of idesia polycarpa. And the method has important practical significance on the popularization of improved varieties of idesia polycarpa, the preservation of germplasm resources and the improvement of industrial quality and efficiency.

The invention discloses a phoebe bournei stem in-vitro rapid propagation method, and relates to the technical field of forest tree tissue culture.The phoebe bournei semi-wood branch is used as an explant, two reagents are used for double-effect sterilization, and a sterile material is rapidly and efficiently obtained; plant growth regulators with different component combinations and concentrations are added for systematic research on axillary bud induction culture, adventitious bud subculture multiplication culture and rooting culture, and browning prevention and control and strong seedling culture tests are further carried out aiming at the browning phenomenon of phoebe bournei cluster buds in the adventitious bud subculture multiplication culture process; according to the method, effective measures for preventing phoebe bournei subculture browning and a method for improving seedling quality are screened, a phoebe bournei stem in-vitro rapid propagation technical system is successfully constructed, a reliable method is provided for phoebe bournei precious resource preservation and large-scale propagation, and a technical reference is provided for in-vitro culture research of other phoebe tree species.

The invention discloses a centella asiatica polyploidy seedling factory production method based on micro-organ cutting-water culture combination, which comprises the following steps: (1) selecting tetraploid centella asiatica aseptic seedlings as materials, transplanting rooting seedlings of the tetraploid centella asiatica aseptic seedlings into nutrient soil for culture, and cutting growing stolons into stem segments with axillary buds; (2) micro-organ cutting culture: cutting the stem sections treated in the step (1) on an improved MS solid culture medium for culture; simultaneously germinating new buds and new roots; transferring the seedlings into an improved MS liquid culture medium for water culture, transferring the obtained seedlings to outdoor for seedling hardening, and then performing field transplanting and field planting. The invention provides an efficient, economic and large-scale propagation scheme for new germplasm after genetic improvement of centella asiatica, so that large-scale industrialized production can be realized in a short time, and the method has a wide industrial application prospect.

The invention provides a method for inhibiting the growth of axillary buds after tobacco topping, which utilizes an axillary bud specific promoter to drive the overexpression of NtMYB306 protein in tobacco, so that the growth of the axillary buds after tobacco topping is inhibited, the growth of tobacco main stem leaves is further promoted, and the growth quality and yield of the tobacco leaves are improved.

The invention discloses a pruning method for promoting blackberry fruiting under an open field efficient cultivation condition, which is used for performing the following pruning management on the blackberries cultivated in the open field in winter after fruits are harvested: (1) pruning basal branches: for the basal branches of the blackberries, selecting 1-2 branches with the branch thickness of more than 20mm, reserving 1-2 branches as trunks, and directly pruning and removing the rest basal branches; (2) secondary lateral branch pruning: retaining 4-5 secondary lateral branches with the thickness of more than 10 mm and the length of more than 80 cm on the basal branches, pruning and removing all the other secondary branches, pruning the retained secondary lateral branches, and controlling the length between 70 cm and 80 cm; 15-20 tertiary lateral branches with the thickness being 7 mm or above and the length being 50 cm or above on the secondary lateral branches are reserved, moderate pruning is conducted on the tertiary lateral branches, the length is controlled to range from 45 cm to 50 cm, 3-5 axillary buds are reserved on each branch, and the other tertiary lateral branches are directly pruned and removed. According to the method, the blackberries cultivated in the open field are pruned in winter, meanwhile, fertilizer and water regulation in spring and summer of the next year is combined, the balance between vegetative growth and reproductive growth of the blackberries can be effectively regulated, flower bud differentiation of the blackberries is promoted, the number and quality of blackberry fruits are improved, and the maximum economic benefits are achieved.

The invention relates to the technical field of agriculture, in particular to a chrysanthemum hardwood cutting propagation method which comprises the following steps: selecting main branches containing axillary buds at the lower parts of chrysanthemum plants at the last flowering stage of autumn, and pruning the main branches into cutting slips according to the standard that the length is 6-8cm, the main branches contain 3-4 sections, and 1-2 leaves are reserved at the upper parts; turf and perlite are evenly mixed according to the volume ratio of (2-3): 1, a potassium permanganate solution is used for disinfection for 48-72 hours, the mixture is laid on a cutting bed after disinfection is completed, and the laying thickness ranges from 8 cm to 10 cm; the cutting slips are inserted into the substrate, the growth temperature of the cutting slips is kept at 17-25 DEG C in the daytime and 5-12 DEG C at night, and the humidity is kept at 70-80%. Main branches containing axillary buds at the lower parts of chrysanthemum plants at the last flowering stage of autumn are selected as cutting slips, so that the survival rate of hard wood cutting of chrysanthemum can be effectively improved, and the root index, plant height and dry weight of cutting seedlings are improved.

The application discloses a kind of methods for efficient rapid propagation of gem slippers oakleaf hydrangea, belong to the technical field of plant rapid propagation.The current year's healthy stem section with axillary bud or apical bud of gem slippers oakleaf hydrangea is used as explant material, after surface disinfection, inoculated in adventitious bud induction and proliferation medium to carry out induction and proliferation culture of adventitious bud;Then the single bud body cluster after proliferation is separated and transferred to synchronous medium to carry out elongation, seedling and rooting synchronous culture of adventitious bud, after domestication and transplanting, finally obtain the healthy gem slippers oakleaf hydrangea regenerated plant.The beneficial effects of the application are: a kind of gem slippers oakleaf hydrangea propagation method is provided, which is fast, efficient and can maintain the excellent characteristics of the mother plant, and provides certain technical support for germplasm conservation, seedling scale propagation and later genetic improvement of gem slippers oakleaf hydrangea.

The application discloses a method for disinfecting and inducing regeneration of a single excellent plant of Malus baccata, and comprises the following steps: S1, disinfecting the terminal bud or / and axillary bud of the Malus baccata; S2, inoculating the terminal bud or / and axillary bud into a modified MS culture medium, and culturing under preset conditions to obtain uncontaminated terminal bud or / and axillary bud; S3, inoculating the uncontaminated terminal bud or / and axillary bud into an induction culture medium, and culturing under preset induction conditions to obtain a cluster bud, wherein the components of the induction culture medium comprise a modified MS as a base, and 6-BA, IPA, prochloraz, cysteine hydrochloride and L-arginine are added; and S4, dividing the cluster bud into single plants, inoculating the single plants into a subculture medium, and culturing under preset culture conditions to obtain Malus baccata single seedlings. Compared with the prior art, the method selects a disinfection method with a high survival rate and a modified MS culture medium with obvious plant life characteristics by taking the terminal bud or / and axillary bud of the Malus baccata as explant material, so that more uncontaminated Malus baccata terminal buds or / and axillary buds which grow well can be obtained, and the tissue culture can be carried out for a long time, thereby providing more materials for the tissue culture of the Malus baccata.

The application discloses a guayule propagation culture medium and a tissue culture rapid propagation method thereof, and belongs to the technical field of tissue culture, and the culture medium comprises MS+0.07 mg / L 6-BA+1 g / L activated carbon+2 g / L agar. The method comprises the following steps: primary induction culture: stem segment explants are induced into seedlings; propagation culture: tissue culture seedlings obtained through the primary culture are selected, stems of axillary buds are taken, and subculture propagation culture is carried out to obtain multiple shoots; rooting culture: the multiple shoots are cultured to obtain rooted tissue culture seedlings; and transplanting: the rooted tissue culture seedlings are transplanted to obtain seedlings. The application can solve the technical problems that the subculture propagation process is extremely easy to vitrify and the propagation coefficient is low, can solve the problems that the excellent seed source of guayule is stable in property and rapid propagation, and realizes large-scale production of high-quality and non-toxic guayule seedlings.

The application discloses a method for constructing a Vietnamese terminalia regenerative system, and comprises the following steps: Vietnamese terminalia stem segment test materials are obtained by grafting seedling saplings with superior branches, semi-lignified stem segments with axillary buds are used as explants, and after disinfection, the explants are inoculated into an induction culture medium to induce axillary buds, then the induced axillary buds are subjected to micro-branch cutting to carry out proliferation and rooting culture, and a large number of Vietnamese terminalia regenerative plants can be obtained. The method has the advantages of high propagation efficiency, high phenotype consistency, good genetic stability and the like, greatly shortens the production cycle of Vietnamese terminalia seedlings, reduces the variation risk of the seedlings, and provides high-quality seedlings for ecological afforestation and landscaping.

The invention discloses a synergistic auxiliary agent for improving the tobacco axillary bud inhibition rate of a bud inhibitor and a preparation method and application thereof, the synergistic auxiliary agent is prepared by compounding plant polyphenol, potassium fulvate and an organic silicon defoamer according to the mass ratio of (14-16): (8-10): 1, and the synergistic auxiliary agent can be cooperatively used with a C12-C14 mixed alcohol bud inhibitor. By enhancing the wetting selectivity of the liquid medicine on tobacco axillary bud villus, the bud inhibition rate is remarkably increased, and the risk of phytotoxicity is reduced. Field tests show that after compounding, the bud inhibition rate reaches 97% or above, the fresh and heavy bud inhibition effect reaches 96%, and the cost is reduced by 30%-40% compared with that of a traditional chemical agent. The method has the advantages of environmental protection, safety and economy, and is suitable for organic tobacco production.

The present invention belongs to the field of plant culture technology, and in particular to a method for inducing the growth of adventitious roots from the terminal buds of a Masson pine adult tree, the method comprising: (1) generating axillary buds by terminal bud induction culture; (2) generating adventitious roots by axillary bud induction culture; wherein, in step (1), a first culture medium is used for cultivation, and the first culture medium is a DCR culture medium containing 6-benzylaminopurine. The method does not need to be rejuvenated, saves steps and time compared with existing adult tree regeneration methods, and inducing axillary buds and breeding from the terminal buds, the bud induction rate and the single bud induction rate are high, and the growth is good; in addition, the adventitious root hairs obtained by the method are numerous, and the roots are thick and strong.

The invention discloses a clematis tissue culture method which comprises the following steps: (1) sterilizing a clematis explant, and inoculating the clematis explant to an axillary bud induction culture medium with the morphological lower end upward for culture; (2) inoculating the explant cultured in the step (1) to a hormone-free culture medium for culturing; (3) inoculating the explant cultured in the step (2) to a rooting culture medium for culturing; and (4) domesticating and transplanting the explants cultured in the step (3) to obtain clematis plants. By means of the tissue culture method, high-quality rapid rooting of the clematis explant can be achieved, meanwhile, a complete plant with a thick and strong root system can be effectively induced, weather conditions do not need to be considered, a large number of high-quality clematis seedlings can be produced within a short time, market requirements are met, and great benefits are brought to industrial production.

The invention relates to the technical field of rice high-yield cultivation, in particular to a regulation and control method for promoting axillary bud differentiation and increasing yield of ratooning rice. The regulation and control method comprises the following steps that S1, 7-10 days before ratooning rice is harvested in the first season, an axillary bud differentiation promoting regulation factor A is sprayed, and the axillary bud differentiation promoting regulation factor A comprises S-abscisic acid, 6-benayl aminopurine and methionine; s2, 2-5 days after harvesting in the first season, an axillary bud differentiation promoting regulatory factor B is sprayed, and the axillary bud differentiation promoting regulatory factor B comprises gibberellic acid, S-abscisic acid, 6-benayl aminopurine and methionine; and S3, when 30-50% of the crevasse in the regeneration season, a yield-increasing-promoting regulation factor C is sprayed, and the yield-increasing-promoting regulation factor C comprises gibberellic acid, S-abscisic acid and methionine. The regulation and control method is applied to ratooning rice, can regulate differentiation of axillary buds of inverted stumps of rice, improve germination uniformity of axillary buds in the regeneration season and the basic seedling number in the field, increase the number of effective ears in the regeneration season, promote filling and fruiting, and improve the yield.