178 results about "Axillary bud" patented technology

The invention discloses an application of an RhRAP2.4 gene in regulation of axillary bud germination of Chinese rose. Relates to the technical field of biology, and provides application of a product for inhibiting expression of an RhRAP2.4 gene or transcriptional translation protein of the RhRAP2.4 gene in promoting germination of rosa chinensis axillary buds, and the nucleotide sequence of the RhRAP2.4 gene is shown as SEQ ID NO.1. According to expression results of the RhRAP2.4 in different site buds of rosa chinensis branches, the RhRAP2.4 in the axillary buds is instantaneously silenced, and the rosa chinensis axillary bud germination is promoted. It is found that abnormal expression of the RhRAP2.4 can influence germination and growth of the axillary buds, silent RhRAP2.4 can inhibit growth of the axillary buds, and overexpressed RhRAP2.4 can promote growth of the axillary buds.

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The invention discloses a tissue culture method of a citral type cinnamomum bodinieri stem with an axillary bud, and relates to the technical field of forest tree tissue culture. The tissue culture method comprises the following steps: selecting a current-year semi-lignified stem on a citral type cinnamomum bodinieri excellent single plant for disinfection treatment, and inoculating the stem on an axillary bud primary induction culture medium for axillary bud primary induction culture; after stem axillary buds in the axillary bud primary induction culture medium germinate, stem segments with axillary buds are soaked with a phycocyanobilin solution and then inoculated into the adventitious bud induction culture medium, red light irradiation treatment is conducted, conventional adventitious bud induction culture is conducted after red light irradiation, adventitious bud subculture multiplication culture, strong seedling culture and rooting induction culture are conducted, and the stem segments with the axillary buds are obtained. And finally, hardening seedlings and transplanting. According to the tissue culture method provided by the invention, the adventitious bud multiplication coefficient can reach more than 5, the rooting rate reaches up to 90.00%, the average rooting number also reaches 3.44, and the survival rate after two months is more than 85%. The invention provides support for better development and utilization of citral type Cinnamomum bodinieri, and has important significance for popularization and application of citral type Cinnamomum bodinieri.

The invention relates to the technical field of agricultural breeding, in particular to a bamboo reed saline-alkaline resistant screening and stress-resistant tissue culture seedling raising method which comprises the following steps: step 1, pretreatment; step 2, dynamic salt stress screening: placing the seed stems in a mixed salt-alkali solution with gradient increasing, culturing, measuring the bud length and root length growth rate of axillary buds of the seed stems, fitting a salt concentration-growth rate curve, and extracting a semi-inhibitory concentration IC50 and a maximum tolerance concentration MTC; step 3, salt tolerance grade classification; 4, carrying out stress-resistant tissue culture seedling culture: carrying out in-vitro culture on the axillary bud explant of the high-salt-resistant variety in a culture medium, and inducing clumpy buds and rooting in combination with a salt-resistant inducer; 5, domestication and transplanting: performing gradual salt stress domestication on the tissue culture seedlings, transplanting the tissue culture seedlings into a saline-alkaline environment, and monitoring the adaptability of the tissue culture seedlings in the saline-alkaline environment. According to the method disclosed by the invention, the efficient cultivation system of the suitable-to-grow bamboo reed seedlings in the saline-alkali soil is established by fusing dynamic stress response analysis and stress resistance phenotype directional strengthening technologies.

The invention discloses a dendrobium officinale tissue culture and rapid propagation seedling raising method, which belongs to the field of biotechnology, and comprises the following steps: S1, explant treatment: selecting a dendrobium officinale plant with good growth vigor and no obvious pest and disease damage, carrying out high temperature stress treatment at 40-45 DEG C for 6-8 days, then taking the tender stem of the dendrobium officinale plant subjected to high temperature stress treatment as the explant, and carrying out tissue culture and rapid propagation on the explant; directly transferring the explant to a low temperature of 2-6 DEG C for stress treatment for 8-12 hours; s2, callus induction: taking the explant treated in the step S1, disinfecting the explant, taking the explant without axillary buds, cutting the explant into small blocks, inoculating the small blocks to a callus induction culture medium, and carrying out dark culture for 12-15 days; the callus induction culture medium comprises 1.5 to 3 mg / L of MS + 2, 4-D, 2 to 3 mg / L of KT and 0.1 to 0.3 mg / L of NAA; s3, bud differentiation induction and rooting induction. According to the tissue culture and rapid propagation method for the dendrobium officinale, the browning rate of the callus during callus induction can be effectively reduced, and the tissue culture seedling raising efficiency is improved.

The invention discloses application of an OsDrAp1 gene in regulation and control of ratooning rice axillary bud germination, and belongs to the technical field of biology. The nucleotide sequence of the OsDrAp1 gene is shown as SEQ ID NO.1. According to the invention, by observing the regeneration bud germination of a knockout and overexpression material of the OsDrAp1, the germination rate of a knockout plant is obviously improved, and the germination rate of the overexpression material is obviously reduced, so that the OsDrAp1 is proved to negatively regulate the regeneration rice axillary bud germination, and the OsDrAp1 can be applied to the breeding research of the regeneration rice.

The invention discloses an artemisia apiacea tissue culture and one-step seedling forming method, which comprises the following steps: cutting stems with axillary buds of artemisia apiacea as explants, and carrying out sterilization and inoculation pretreatment; inoculating the explants on an induction culture medium for culturing, and inducing the explants to generate cluster buds; transferring the germinated cluster buds to a proliferation culture medium for proliferation culture of the cluster buds; and cutting the proliferated non-rooted seedlings, and transferring to a rooting culture medium for rooting culture. According to the method, single high-concentration excitation 6-BA is used as a hormone for inducing the culture medium, axillary bud stem explants can be directly induced to generate cluster buds, and the generation of calluses is remarkably reduced. Meanwhile, in the subculture multiplication culture, after the subculture stage and the concentration of the used 6-BA are flexibly adjusted, the generation of calluses and the generation of vitrification phenomena can be further reduced. According to the method, the cluster buds can be rapidly rooted, so that a large number of seedlings are obtained, and the method has the remarkable advantages of saving time and manpower and material resource cost in production.

This disclosure provides a number of sequences involved in axillary bud growth in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences or transgenes of such sequences, and tobacco products made from tobacco leaf harvested from such plants.

The invention discloses a tomato axillary bud real-time identification, measurement and removal point positioning method. The method comprises the following steps: acquiring RGB and depth images of a tomato plant by using a mobile phone or RGB-D sensor equipment; a lightweight model is adopted to carry out instance segmentation on the RGB image, and axillary buds and branches are identified and segmented. And then, aligning the binary mask of the axillary bud with the depth image, constructing corresponding three-dimensional point cloud data, and calculating the length of the axillary bud and judging the removability based on whether the point cloud and the axillary bud region contain axillary bud bifurcation. And after determining that the axillary buds can be removed, further determining axillary bud removal points. By means of the method, agricultural management equipment can rapidly, automatically and accurately obtain the axillary bud length and judge the removability of the axillary bud, and therefore tomato pruning operation is efficiently completed.

This invention discloses a method for rapid seedling cultivation of Eucommia ulmoides via tissue culture, relating to the field of Eucommia ulmoides breeding technology. The method includes the following steps: using young, tender stem segments with axillary buds from superior Eucommia ulmoides strains as explants, after surface disinfection, the segments are cut into sections with axillary buds and inoculated into an axillary bud induction medium to induce axillary bud germination; the germinated axillary buds are cut into segments and inoculated into a specific medium for adventitious bud induction and elongation culture; the adventitious buds after proliferation culture are separated and subjected to a two-step in-bottle rooting method or external adventitious root induction, ultimately obtaining complete regenerated Eucommia ulmoides plants. Beneficial effects: This invention develops a simplified Eucommia ulmoides tissue culture propagation method that shortens the production cycle, has a high propagation coefficient, rapid rooting, high adventitious root induction rate, and minimal basal callus. It can rapidly obtain high-quality Eucommia ulmoides seedlings, which is of great significance for the large-scale propagation of superior strains and varietal improvement research, while also providing technical support for the sustainable development of the Eucommia ulmoides industry.

The invention discloses a method for in-vitro preservation of common wild rice and Nwa wild rice, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a stem node with an axillary bud as an explant; soaking in 75% alcohol for 10-15 seconds, soaking in 10% 84 disinfectant for 15 minutes, performing vacuum treatment in 0.1% mercuric chloride solution under-0.1 Mpa for 5 minutes, and performing water bath at 40 DEG C for 20 minutes for combined sterilization; inserting the sterilized explant into a bud differentiation culture medium containing 2mg / L KT and 0.1 mg / L NAA, and culturing for 3-5 days under the conditions that the temperature is 23 DEG C and illumination is performed for 16 hours per day; cutting off buds growing to 3-5cm, and transferring the buds into a rooting culture medium; (5) carrying out test-tube plantlet propagation through stem node shearing or tillering cutting; the tissue culture seedlings with well developed root systems are hardened and then transplanted to a mixture of vermiculite and nutrient soil (v / v = 1 / 1). According to the method, the pollution rate is as low as 16.35%, the differentiation rate of the explant reaches 47.36%, the process is simple, convenient and efficient, the production period is short, the seed quality resources of common wild rice and Nilval wild rice can be effectively preserved, and the method has important significance on protection of precious wild rice resources.

The invention relates to the technical field of chrysanthemum breeding methods, in particular to a method for accelerating chrysanthemum breeding by using a liquid culture medium, which comprises the following steps: cutting chrysanthemum stalks into square blocks of 1cm * 1cm in a culture box, flatly laying the stalks upwards on an induction culture solution soaking base, cutting stem tissues with axillary buds into small sections of 2-3cm, and obliquely inserting the small sections into the induction culture solution for culturing, mS + 6-BA 2.0 mg / L +, NAA 0.1 mg / L, alpha-naphthylacetic acid 2-4 mg / L, 6-benzylaminopurine and cane sugar are selected as culture solutions, in the propagation culture of the chrysanthemum, stem seedling sections grow fast, each section generates a plurality of clustered seedlings, the average multiplication coefficient is 4-7 times, the height of the stem seedlings is different, the clustered seedlings are 5-7 cm high when the seedlings are cultured in the nutrient solution for 2 months, and the adventitious bud multiplication rate of the chrysanthemum seedlings is increased. The method has the effect of accelerating chrysanthemum reproduction.

The invention relates to the technical field of seedling raising, and discloses an efficient tissue culture and rapid propagation seedling raising method for xanthoceras sorbifolia bunge, which comprises the following steps: selecting and pretreating explants, selecting current-year robust branches of the xanthoceras sorbifolia bunge, cutting stem segments with axillary buds as the explants, cleaning, trimming and disinfecting the explants, performing surface disinfection on the pretreated explants, and performing tissue culture and rapid propagation seedling raising on the pretreated explants to obtain the xanthoceras sorbifolia bunge. When the Xanthoceras sorbifolia Bunge is subjected to efficient tissue culture and rapid propagation seedling culture, an operation standard considering thorough sterilization and tissue activity protection is established by standardizing an explant selection standard and executing a step-by-step surface disinfection procedure, so that the microbial contamination rate caused by incomplete disinfection of the explant can be reduced, meanwhile, tissue activity damage caused by excessive disinfection is reduced, and the survival rate of the Xanthoceras sorbifolia Bunge is improved. Therefore, sufficient and healthy sterile initial materials are guaranteed to be obtained, the problems of high primary culture pollution rate and induction starting failure are solved, and a reliable foundation is laid for establishment of a whole tissue culture and rapid propagation system.

The invention discloses a tissue culture method of a medicinal plant divaricate saposhnikovia root. The method comprises the following steps: taking a stem section with buds as an explant, adding 30g / L of sucrose, 8g / L of agar and 0.5 mg / L of 6-BA into an MS (Murashige and Skoog) culture medium as an induction culture medium, and ensuring that the pH value is A rooting culture medium is formed by adding 30 g / L of sucrose, 8 g / L of agar and 0.5 mg / L of IBA into an MS culture medium, and the pH value is 5.8. By using the method, the axillary bud induction rate is 100%, the multiplication coefficient reaches 2.29, and the average stem height is 1.1 cm; the rooting rate reaches 91%, the rooting coefficient reaches 17.25, and the formed roots are dense and thick and are suitable for transplanting. The method not only accelerates the breeding speed of the divaricate saposhnikovia root, but also keeps excellent characteristics of parents, is of great significance to rapid breeding and genetic improvement, and lays a technical foundation for commercial production.

The invention discloses a method for in-vitro induction of tulip tree cluster buds and regeneration seedlings. The method comprises the following steps: firstly, cutting 3-4cm of a plant material containing terminal buds or axillary buds from a liriodendron twig, disinfecting, removing plant endophyte, then cutting 0.5-1cm of the terminal buds or axillary buds as explants, then putting the explants on a cluster bud induction culture medium for induction culture for about 30 days, then cutting off the induced cluster buds, and putting the induced cluster buds on an elongation culture medium for culture; when the buds extend to be rootless seedlings with the length of 2-3 cm, the seedlings are transferred to a rooting culture medium for rooting induction, and the seedlings can root after being cultured in the rooting culture medium for about 40 days. According to the method, terminal buds and axillary buds are used as explants, growth of endophytes is effectively controlled, a cluster bud induction culture medium, a bud elongation culture medium and a rooting culture medium are screened out, an in-vitro regeneration system is established, and technical support is provided for propagation of liriodendron plants.

The invention relates to the technical field of plant genetic engineering, and discloses a strawberry stolon regulation gene ZFP6 and application thereof, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. Researches prove that the ZFP6 gene is specifically expressed in axillary meristem of strawberry in the adult-plant stage, and the GA20ox4 gene can be directly or indirectly activated to start a biosynthesis pathway of GA and drive axillary buds to develop towards stolons. Through genetic interaction analysis of the ZFP6 gene, the GA20ox4 gene and the RGA1 gene, the function of the ZFP6 gene as an axillary bud fate decision upstream regulation factor is further verified, the blank of an endogenous regulation mechanism of the strawberry stolons is filled, and the ZFP6 gene is proved to be a molecular target for specifically regulating formation of the strawberry stolons. The gene sequence, the primer, the gene knockout expression vector and the matched technical system provided by the invention lay a foundation for strawberry molecular breeding and industrial application.

The present application relates to asparagus tissue culture technical field, specifically to a kind of asparagus tissue culture rapid propagation seedling cultivation method.A kind of asparagus tissue culture rapid propagation seedling cultivation method, comprising: the disinfection and browning prevention treatment of explant, preparation of composite antibacterial agent, preparation of culture medium, asparagus explant culture and transplanting.The present application adopts ozone fumigation to combine ultraviolet light disinfection, synergistic sterilization, covers surface and deep layer, reduces explant injury and browning.Browning prevention agent inhibits polyphenol oxidase, removes free radicals, reduces browning, promotes axillary bud germination.Add composite antibacterial agent in induction culture medium, destroys microbial cell membrane, has antibacterial and antioxidant effects, and guarantees explant growth.Compound rooting agent contains activated carbon, chitosan and sucrose, adsorbs harmful substances, stimulates root differentiation and provides energy, improves rooting rate and plant robustness.Overall, it improves the success rate of asparagus tissue culture.

This invention relates to the field of gene regulation technology, specifically to the application of the RhATHB12 gene in regulating axillary bud germination in roses, along with its kit and regulation method. The nucleotide sequence of the RhATHB12 gene is shown in SEQ ID NO.1. Regulation involves silencing, knocking out, or inhibiting the expression of the RhATHB12 gene, which may promote axillary bud germination; overexpression of RhATHB12, which may inhibit axillary bud germination. This gene and its regulation method are of paramount importance for cut rose breeding and for improving the quality and efficiency of the industry.

The invention discloses a phantom liquidambar formosana axillary bud tissue culture proliferation method, which takes phantom liquidambar formosana axillary buds as tissue culture materials and improves the propagation speed of phantom liquidambar formosana seedlings. After axillary buds of the 'phantom' liquidambar formosana hance are collected and treated, the most suitable culture medium and growth regulator are screened through primary culture, subculture and rooting culture, so that tissue culture proliferation of the 'phantom' liquidambar formosana hance is completed. According to the method, the population cardinal number of the phantom liquidambar formosana hance can be rapidly increased, the problem that the grafting speed is not high due to the fact that branches of the phantom liquidambar formosana hance are thickened slowly is solved, and a clonal germplasm resource nursery of the phantom liquidambar formosana hance can be built early.

The invention discloses a plant tissue culture method of edible chrysanthemum, and relates to the technical field of tissue culture of edible chrysanthemum, and the plant tissue culture method comprises the following steps: selecting an edible chrysanthemum tender stem as an explant, washing with an SDS surfactant, flushing with running water, placing under an ultra-clean sterile condition, disinfecting for 10 minutes with a 0.1% mercury bichloride solution, shaking, then flushing with sterile water, and drying to obtain the plant tissue culture method of the edible chrysanthemum. Placing on sterile absorbent paper for dry absorption, cutting into segments with 1-2 leaves and length of 2-3cm, and vertically inserting the lower parts of the segments into a rooting culture medium; the method comprises the following steps: carrying out sterile rapid cultivation, starting tender stem culture, growing green protuberant differentiated tender stems on the fifth day, and enabling the plant height to be 3-5cm after about one month, and carrying out subculture proliferation, namely shearing the tender stems into small sections with one section, one bud and one leaf, inserting the base parts of the cut sections into a culture medium, forming axillary buds into new plants after 3-4 weeks, and repeatedly shearing and propagating. The edible chrysanthemum tissue culture method is high in propagation efficiency, rapid and large-scale propagation can be realized, excellent characters of female parents can be kept, the method is not limited by seasons, annual production can be realized, the survival rate and quality of seedlings are ensured, and the stress resistance is enhanced.

The present invention relates to the technical field of fruit industry, and specifically discloses a regulation method and application for promoting and strengthening the flower formation of citrus. The regulation method is to spray flower-promoting regulator A during the ripening stage of autumn shoots of citrus; spray flower-promoting regulator B from 1 week before the appearance of flower buds in the following year to the flower bud stage of citrus. The components and spraying concentration of the flower-promoting regulator A are: S-abscisic acid 15-50 μg / ml, prohexadione-calcium 25-100 μg / ml, chlorophyllin iron 0.05-1 μg / ml, and the balance is an agriculturally acceptable adjuvant; the components and spraying concentration of the flower-promoting regulator B are: S-abscisic acid 1-35 μg / ml, 6-benzylaminopurine 10-70 μg / ml, and chlorophyllin iron 0.1-2 μg / ml, and the balance is an agriculturally acceptable adjuvant. The present invention can regulate the balance of flower-forming hormones in axillary buds of the tree body, effectively increase the flower formation amount of citrus in the following year, improve the quality of flower buds and the flowering uniformity, reduce the proportion of deformed flowers, reduce the difficulty of fruit retention, increase the citrus yield and improve the quality.

The invention provides an idesia grafting seedling raising method, relates to the technical field of grafting seedling raising, and adopts the technical scheme that the method comprises the steps of scion type determination, grafting method determination, grafting time determination and the like. According to the statistical data of the survival rate, the determination of appearance growth indexes of the grafted seedlings, and the fruit yield and quality, the optimal grafting mode is determined to be grafting in an axillary bud cut-grafting mode at the beginning of spring. The grafting combination most suitable for idesia polycarpa branch grafting is researched from different grafting periods and different grafting methods, a set of grafting method suitable for idesia polycarpa branches is established, and a set of feasible grafting method capable of achieving industrialized seedling raising is provided for asexual propagation of idesia polycarpa. And the method has important practical significance on the popularization of improved varieties of idesia polycarpa, the preservation of germplasm resources and the improvement of industrial quality and efficiency.

The cherry tomato planting method comprises a step of pruning branches with double stems so as to obtain two main branches, and is characterized by further comprising a step of repeatedly pinching and thinning buds on the main branches when inflorescence of a second spike is obviously visible, and specifically, the first spike inflorescence and the second spike inflorescence are obviously visible. When two inflorescences grow on the main branch and the length of the inflorescence of the next inflorescence is more than 3cm or three buds exist on the main branch, performing terminal bud pinching treatment on a new shoot which normally grows on the inflorescence of the next inflorescence, meanwhile, reserving axillary buds of adjacent leaves under the inflorescence of the next inflorescence as extension branches of the main branch for culture, and thinning all the rest axillary buds. In the step of repeated pinching and bud thinning, pinching is carried out every two ear inflorescences, so that the ear length and the fruit setting rate of a main branch from the first ear inflorescence to the last ear inflorescence are gradually increased, and the fruit setting rate and the yield of cherry tomato plants are effectively improved; the inflorescence is treated every two spikes, so that the inflorescence stretching, flowering and fruit setting capacities are guaranteed to the maximum extent, and meanwhile, the moderate growth vigor of the plants is well coordinated.

The invention discloses a phoebe bournei stem in-vitro rapid propagation method, and relates to the technical field of forest tree tissue culture.The phoebe bournei semi-wood branch is used as an explant, two reagents are used for double-effect sterilization, and a sterile material is rapidly and efficiently obtained; plant growth regulators with different component combinations and concentrations are added for systematic research on axillary bud induction culture, adventitious bud subculture multiplication culture and rooting culture, and browning prevention and control and strong seedling culture tests are further carried out aiming at the browning phenomenon of phoebe bournei cluster buds in the adventitious bud subculture multiplication culture process; according to the method, effective measures for preventing phoebe bournei subculture browning and a method for improving seedling quality are screened, a phoebe bournei stem in-vitro rapid propagation technical system is successfully constructed, a reliable method is provided for phoebe bournei precious resource preservation and large-scale propagation, and a technical reference is provided for in-vitro culture research of other phoebe tree species.

The invention discloses a centella asiatica polyploidy seedling factory production method based on micro-organ cutting-water culture combination, which comprises the following steps: (1) selecting tetraploid centella asiatica aseptic seedlings as materials, transplanting rooting seedlings of the tetraploid centella asiatica aseptic seedlings into nutrient soil for culture, and cutting growing stolons into stem segments with axillary buds; (2) micro-organ cutting culture: cutting the stem sections treated in the step (1) on an improved MS solid culture medium for culture; simultaneously germinating new buds and new roots; transferring the seedlings into an improved MS liquid culture medium for water culture, transferring the obtained seedlings to outdoor for seedling hardening, and then performing field transplanting and field planting. The invention provides an efficient, economic and large-scale propagation scheme for new germplasm after genetic improvement of centella asiatica, so that large-scale industrialized production can be realized in a short time, and the method has a wide industrial application prospect.

The invention provides a method for inhibiting the growth of axillary buds after tobacco topping, which utilizes an axillary bud specific promoter to drive the overexpression of NtMYB306 protein in tobacco, so that the growth of the axillary buds after tobacco topping is inhibited, the growth of tobacco main stem leaves is further promoted, and the growth quality and yield of the tobacco leaves are improved.