89 results about "Axillary bud" patented technology

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

This invention discloses a method for rapid seedling cultivation of Eucommia ulmoides via tissue culture, relating to the field of Eucommia ulmoides breeding technology. The method includes the following steps: using young, tender stem segments with axillary buds from superior Eucommia ulmoides strains as explants, after surface disinfection, the segments are cut into sections with axillary buds and inoculated into an axillary bud induction medium to induce axillary bud germination; the germinated axillary buds are cut into segments and inoculated into a specific medium for adventitious bud induction and elongation culture; the adventitious buds after proliferation culture are separated and subjected to a two-step in-bottle rooting method or external adventitious root induction, ultimately obtaining complete regenerated Eucommia ulmoides plants. Beneficial effects: This invention develops a simplified Eucommia ulmoides tissue culture propagation method that shortens the production cycle, has a high propagation coefficient, rapid rooting, high adventitious root induction rate, and minimal basal callus. It can rapidly obtain high-quality Eucommia ulmoides seedlings, which is of great significance for the large-scale propagation of superior strains and varietal improvement research, while also providing technical support for the sustainable development of the Eucommia ulmoides industry.

The invention discloses a method for in-vitro preservation of common wild rice and Nwa wild rice, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a stem node with an axillary bud as an explant; soaking in 75% alcohol for 10-15 seconds, soaking in 10% 84 disinfectant for 15 minutes, performing vacuum treatment in 0.1% mercuric chloride solution under-0.1 Mpa for 5 minutes, and performing water bath at 40 DEG C for 20 minutes for combined sterilization; inserting the sterilized explant into a bud differentiation culture medium containing 2mg / L KT and 0.1 mg / L NAA, and culturing for 3-5 days under the conditions that the temperature is 23 DEG C and illumination is performed for 16 hours per day; cutting off buds growing to 3-5cm, and transferring the buds into a rooting culture medium; (5) carrying out test-tube plantlet propagation through stem node shearing or tillering cutting; the tissue culture seedlings with well developed root systems are hardened and then transplanted to a mixture of vermiculite and nutrient soil (v / v = 1 / 1). According to the method, the pollution rate is as low as 16.35%, the differentiation rate of the explant reaches 47.36%, the process is simple, convenient and efficient, the production period is short, the seed quality resources of common wild rice and Nilval wild rice can be effectively preserved, and the method has important significance on protection of precious wild rice resources.

The invention relates to the technical field of seedling raising, and discloses an efficient tissue culture and rapid propagation seedling raising method for xanthoceras sorbifolia bunge, which comprises the following steps: selecting and pretreating explants, selecting current-year robust branches of the xanthoceras sorbifolia bunge, cutting stem segments with axillary buds as the explants, cleaning, trimming and disinfecting the explants, performing surface disinfection on the pretreated explants, and performing tissue culture and rapid propagation seedling raising on the pretreated explants to obtain the xanthoceras sorbifolia bunge. When the Xanthoceras sorbifolia Bunge is subjected to efficient tissue culture and rapid propagation seedling culture, an operation standard considering thorough sterilization and tissue activity protection is established by standardizing an explant selection standard and executing a step-by-step surface disinfection procedure, so that the microbial contamination rate caused by incomplete disinfection of the explant can be reduced, meanwhile, tissue activity damage caused by excessive disinfection is reduced, and the survival rate of the Xanthoceras sorbifolia Bunge is improved. Therefore, sufficient and healthy sterile initial materials are guaranteed to be obtained, the problems of high primary culture pollution rate and induction starting failure are solved, and a reliable foundation is laid for establishment of a whole tissue culture and rapid propagation system.

The invention relates to the technical field of plant genetic engineering, and discloses a strawberry stolon regulation gene ZFP6 and application thereof, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. Researches prove that the ZFP6 gene is specifically expressed in axillary meristem of strawberry in the adult-plant stage, and the GA20ox4 gene can be directly or indirectly activated to start a biosynthesis pathway of GA and drive axillary buds to develop towards stolons. Through genetic interaction analysis of the ZFP6 gene, the GA20ox4 gene and the RGA1 gene, the function of the ZFP6 gene as an axillary bud fate decision upstream regulation factor is further verified, the blank of an endogenous regulation mechanism of the strawberry stolons is filled, and the ZFP6 gene is proved to be a molecular target for specifically regulating formation of the strawberry stolons. The gene sequence, the primer, the gene knockout expression vector and the matched technical system provided by the invention lay a foundation for strawberry molecular breeding and industrial application.

The present application relates to asparagus tissue culture technical field, specifically to a kind of asparagus tissue culture rapid propagation seedling cultivation method.A kind of asparagus tissue culture rapid propagation seedling cultivation method, comprising: the disinfection and browning prevention treatment of explant, preparation of composite antibacterial agent, preparation of culture medium, asparagus explant culture and transplanting.The present application adopts ozone fumigation to combine ultraviolet light disinfection, synergistic sterilization, covers surface and deep layer, reduces explant injury and browning.Browning prevention agent inhibits polyphenol oxidase, removes free radicals, reduces browning, promotes axillary bud germination.Add composite antibacterial agent in induction culture medium, destroys microbial cell membrane, has antibacterial and antioxidant effects, and guarantees explant growth.Compound rooting agent contains activated carbon, chitosan and sucrose, adsorbs harmful substances, stimulates root differentiation and provides energy, improves rooting rate and plant robustness.Overall, it improves the success rate of asparagus tissue culture.

This invention relates to the field of gene regulation technology, specifically to the application of the RhATHB12 gene in regulating axillary bud germination in roses, along with its kit and regulation method. The nucleotide sequence of the RhATHB12 gene is shown in SEQ ID NO.1. Regulation involves silencing, knocking out, or inhibiting the expression of the RhATHB12 gene, which may promote axillary bud germination; overexpression of RhATHB12, which may inhibit axillary bud germination. This gene and its regulation method are of paramount importance for cut rose breeding and for improving the quality and efficiency of the industry.

The invention discloses a phoebe bournei stem in-vitro rapid propagation method, and relates to the technical field of forest tree tissue culture.The phoebe bournei semi-wood branch is used as an explant, two reagents are used for double-effect sterilization, and a sterile material is rapidly and efficiently obtained; plant growth regulators with different component combinations and concentrations are added for systematic research on axillary bud induction culture, adventitious bud subculture multiplication culture and rooting culture, and browning prevention and control and strong seedling culture tests are further carried out aiming at the browning phenomenon of phoebe bournei cluster buds in the adventitious bud subculture multiplication culture process; according to the method, effective measures for preventing phoebe bournei subculture browning and a method for improving seedling quality are screened, a phoebe bournei stem in-vitro rapid propagation technical system is successfully constructed, a reliable method is provided for phoebe bournei precious resource preservation and large-scale propagation, and a technical reference is provided for in-vitro culture research of other phoebe tree species.

The application discloses a kind of methods for efficient rapid propagation of gem slippers oakleaf hydrangea, belong to the technical field of plant rapid propagation.The current year's healthy stem section with axillary bud or apical bud of gem slippers oakleaf hydrangea is used as explant material, after surface disinfection, inoculated in adventitious bud induction and proliferation medium to carry out induction and proliferation culture of adventitious bud;Then the single bud body cluster after proliferation is separated and transferred to synchronous medium to carry out elongation, seedling and rooting synchronous culture of adventitious bud, after domestication and transplanting, finally obtain the healthy gem slippers oakleaf hydrangea regenerated plant.The beneficial effects of the application are: a kind of gem slippers oakleaf hydrangea propagation method is provided, which is fast, efficient and can maintain the excellent characteristics of the mother plant, and provides certain technical support for germplasm conservation, seedling scale propagation and later genetic improvement of gem slippers oakleaf hydrangea.

The application discloses a method for disinfecting and inducing regeneration of a single excellent plant of Malus baccata, and comprises the following steps: S1, disinfecting the terminal bud or / and axillary bud of the Malus baccata; S2, inoculating the terminal bud or / and axillary bud into a modified MS culture medium, and culturing under preset conditions to obtain uncontaminated terminal bud or / and axillary bud; S3, inoculating the uncontaminated terminal bud or / and axillary bud into an induction culture medium, and culturing under preset induction conditions to obtain a cluster bud, wherein the components of the induction culture medium comprise a modified MS as a base, and 6-BA, IPA, prochloraz, cysteine hydrochloride and L-arginine are added; and S4, dividing the cluster bud into single plants, inoculating the single plants into a subculture medium, and culturing under preset culture conditions to obtain Malus baccata single seedlings. Compared with the prior art, the method selects a disinfection method with a high survival rate and a modified MS culture medium with obvious plant life characteristics by taking the terminal bud or / and axillary bud of the Malus baccata as explant material, so that more uncontaminated Malus baccata terminal buds or / and axillary buds which grow well can be obtained, and the tissue culture can be carried out for a long time, thereby providing more materials for the tissue culture of the Malus baccata.

The application discloses a guayule propagation culture medium and a tissue culture rapid propagation method thereof, and belongs to the technical field of tissue culture, and the culture medium comprises MS+0.07 mg / L 6-BA+1 g / L activated carbon+2 g / L agar. The method comprises the following steps: primary induction culture: stem segment explants are induced into seedlings; propagation culture: tissue culture seedlings obtained through the primary culture are selected, stems of axillary buds are taken, and subculture propagation culture is carried out to obtain multiple shoots; rooting culture: the multiple shoots are cultured to obtain rooted tissue culture seedlings; and transplanting: the rooted tissue culture seedlings are transplanted to obtain seedlings. The application can solve the technical problems that the subculture propagation process is extremely easy to vitrify and the propagation coefficient is low, can solve the problems that the excellent seed source of guayule is stable in property and rapid propagation, and realizes large-scale production of high-quality and non-toxic guayule seedlings.

The application discloses a method for constructing a Vietnamese terminalia regenerative system, and comprises the following steps: Vietnamese terminalia stem segment test materials are obtained by grafting seedling saplings with superior branches, semi-lignified stem segments with axillary buds are used as explants, and after disinfection, the explants are inoculated into an induction culture medium to induce axillary buds, then the induced axillary buds are subjected to micro-branch cutting to carry out proliferation and rooting culture, and a large number of Vietnamese terminalia regenerative plants can be obtained. The method has the advantages of high propagation efficiency, high phenotype consistency, good genetic stability and the like, greatly shortens the production cycle of Vietnamese terminalia seedlings, reduces the variation risk of the seedlings, and provides high-quality seedlings for ecological afforestation and landscaping.

The invention discloses a clematis tissue culture method which comprises the following steps: (1) sterilizing a clematis explant, and inoculating the clematis explant to an axillary bud induction culture medium with the morphological lower end upward for culture; (2) inoculating the explant cultured in the step (1) to a hormone-free culture medium for culturing; (3) inoculating the explant cultured in the step (2) to a rooting culture medium for culturing; and (4) domesticating and transplanting the explants cultured in the step (3) to obtain clematis plants. By means of the tissue culture method, high-quality rapid rooting of the clematis explant can be achieved, meanwhile, a complete plant with a thick and strong root system can be effectively induced, weather conditions do not need to be considered, a large number of high-quality clematis seedlings can be produced within a short time, market requirements are met, and great benefits are brought to industrial production.

The invention relates to the technical field of rice high-yield cultivation, in particular to a regulation and control method for promoting axillary bud differentiation and increasing yield of ratooning rice. The regulation and control method comprises the following steps that S1, 7-10 days before ratooning rice is harvested in the first season, an axillary bud differentiation promoting regulation factor A is sprayed, and the axillary bud differentiation promoting regulation factor A comprises S-abscisic acid, 6-benayl aminopurine and methionine; s2, 2-5 days after harvesting in the first season, an axillary bud differentiation promoting regulatory factor B is sprayed, and the axillary bud differentiation promoting regulatory factor B comprises gibberellic acid, S-abscisic acid, 6-benayl aminopurine and methionine; and S3, when 30-50% of the crevasse in the regeneration season, a yield-increasing-promoting regulation factor C is sprayed, and the yield-increasing-promoting regulation factor C comprises gibberellic acid, S-abscisic acid and methionine. The regulation and control method is applied to ratooning rice, can regulate differentiation of axillary buds of inverted stumps of rice, improve germination uniformity of axillary buds in the regeneration season and the basic seedling number in the field, increase the number of effective ears in the regeneration season, promote filling and fruiting, and improve the yield.

The invention discloses a tissue culture method for reducing browning of cercis chinensis, which comprises the following steps: S1, cleaning and disinfecting stems with axillary buds of cercis chinensis, and taking the axillary buds from a sodium sulfite solution; s2, carrying out primary culture on the axillary buds to obtain aseptic seedlings, and carrying out anti-browning culture and growth promoting culture on the aseptic seedlings to obtain cercis chinensis tissue culture seedlings; a culture medium used in the anti-browning culture comprises the following components: improved MS, IBA, 2, 4-D, rutin and sodium sulfite. Compared with the prior art, the method has the advantages that the aseptic seedling obtaining rate is increased by 81.67% by improving the disinfection method, the transplanting survival rate of the cercis chinensis aseptic seedlings obtained through anti-browning growth-promoting culture is 88.33%, and the surviving cercis chinensis aseptic seedlings have dark green or cyan leaves and more robust stems.

The invention provides an efficient asexual propagation method of ficus pandurata suitable for simple condition application. The method comprises the following steps: (1) explant disinfection: soaking a stem with an axillary bud in a composite disinfectant containing mercuric chloride; (2) primary culture: inoculating the disinfected stem into a starting culture medium containing cytokinin and auxin for culture; (3) multiplication culture: transferring the callus formed by primary culture to a differential culture medium to induce cluster buds; (4) carrying out vector rooting, namely cutting robust buds obtained by multiplication culture, and inserting the robust buds into a rooting culture medium containing microencapsulated auxin; and (5) transplanting and hardening seedlings: treating the tissue culture seedlings with a protective solution, and transplanting the treated tissue culture seedlings to a compound matrix. The method can realize substantive transformation from a specialized tissue culture technology to basic-level production, and solves the industrialization bottlenecks such as equipment threshold and operation complexity.

The invention relates to the technical field of plant bud growth pruning. The invention particularly relates to a pine pruning and bud picking tool capable of reducing rosin outflow and a bud picking method. Comprising a barrel, an extrusion rod is slidably arranged in the barrel, a moving ring is slidably arranged at one end of the extrusion rod, connecting strips are hinged to the two sides of the moving ring, extrusion blocks are hinged to the other ends of the connecting strips, the number of the extrusion blocks is two, and a first plate is slidably arranged in the middle of one extrusion block; through rotary extrusion of the extrusion blocks, axillary buds penetrating into the lower part of the cylinder can be extruded, so that axillary bud tissues are extruded and pushed downwards, original ecology in the axillary buds is destroyed, growth of the axillary buds is inhibited, and the axillary buds are prevented from being damaged. And the rotating column is rotatably arranged to clamp the edge part of the axillary bud, so that the axillary bud can be rotatably folded, and the axillary bud pulp is prevented from resetting again.

The invention discloses an acer palmatum twig cuttage method, and belongs to the technical field of plant cuttage, and the method comprises the following steps: controlling a greenhouse environment according to set temperature and humidity conditions; a deepened cuttage hole tray is placed on the cuttage seedling bed, a cuttage medium is formed by mixing vermiculite and perlite according to a set proportion, and after being disinfected, the medium stands in a greenhouse and is loaded into the tray; the method comprises the following steps: selecting current-year semi-lignified coppice shoots, washing the coppice shoots with clear water after harvesting, cutting leaves in half during spike making, reserving a pair of axillary buds, cutting the axillary buds into cutting spike sections with the length of 5-8cm under the buds, and putting the cutting spike sections into a bactericide; soaking the lower part of the cutting bud with a rooting agent for a set time; the cutting depth is 1 / 2 of the length of the spike section, keeping the temperature and humidity in the greenhouse after cutting, and spraying a bactericide and a rooting agent according to set time; after the root system is mature and brown, transplanting into a pot. According to the cuttage method, the rooting period of the acer palmatum is shortened and only needs 20-30 days, and the rooting rate of the acer palmatum is increased and can reach 50% or above.

The invention provides a first season rice harvesting stubble height and ratooning rice yield prediction method, and relates to the technical field of agricultural planting. The method comprises four core modules including first rice variety whole growth period selection, first-season rice harvesting stubble height determination, second-crop rice effective ear calculation and ratooning rice yield prediction, wherein the whole growth period of the first rice variety is determined through a formula (1) and a formula (2) in combination with regional climate characteristics, and the influence of cold dew wind is avoided; a 6-9-point sampling method is adopted to observe the lengths of base nodes and inverted 1-4 nodes of rice plants and axillary bud vitality, empirical values of fixed heights are abandoned, and the heights of low stubbles, medium stubbles and high stubbles are scientifically divided; on the basis of the first rice effective ears, the axillary bud ear percentage and the harvester pressure loss correction rate, second-crop rice effective ears are calculated through a series of formulas (4); and predicting the yield of ratooning rice by using a series of formulas (5) in combination with parameters such as the grain setting rate and the maturing rate. The method realizes whole-process mechanized dry direct seeding and ratooning rice cultivation, can increase the annual yield per unit area, saves the cost, and is suitable for large-scale planting of ratooning rice.

The invention discloses a tissue culture and rapid propagation method of salvia involucrata, and belongs to the technical field of plant rapid propagation. The method provided by the invention comprises the following steps: sterilizing a stem section with a bud point of Salvia involucrata, and then cleaning the stem section with sterile water to serve as an explant; the explants are placed in an axillary bud induction culture medium to be subjected to axillary bud induction multiplication culture, seedlings are obtained, and the axillary bud induction culture medium is a WPM culture medium added with 0.5-1.0 mg / L 6-BA and 0.1-0.2 mg / L NAA; the seedlings are transferred to a rooting culture medium to be cultured, the rooting culture medium is a WPM culture medium added with 0.06-0.08 mg / L of NAA, and rooted tissue culture seedlings are obtained; and hardening the rooted tissue culture seedlings, and transplanting the tissue culture seedlings into a matrix. The method has the advantages of being not limited by environment and seasons, high in propagation speed, large in propagation coefficient, neat and consistent in propagated offspring and high in transplanting survival rate, and has good application prospects.

The invention relates to the technical field of agricultural biology, and discloses an efficient in-vitro regeneration and rapid propagation method for Shannong No.1 rootstocks, and the method comprises the steps of explant selection and pretreatment, sterile disinfection, axillary bud germination and induction, adventitious bud proliferation, rooting culture and acclimatization and transplanting. The browning rate and the pollution rate of the explants are effectively reduced by combining full-dark culture at the initial induction stage; gibberellin A3 is creatively added into a proliferation culture medium to be matched with 6-benzylamino adenine and indole-3-butyric acid, so that a bud seedling vitrification phenomenon is prevented, and stem elongation is promoted; in the rooting stage, dark treatment is combined with high-concentration auxin induction, so that the rooting rate and the root system quality are improved. According to the method, an efficient and stable regeneration system is established, the method has the advantages of high propagation coefficient, robust seedling, high transplanting survival rate and the like, and reliable technical support is provided for large-scale propagation of the Shannong No.1 rootstock.

The invention provides a rice stump rolling treatment method for improving uniformity and grain number per ear of ratooning rice, which comprises the following steps: carrying out field management on first-season rice by adopting a conventional cultivation mode, and harvesting the first-season rice by adopting a combine harvester according to conventional operation; after harvesting in the first season is completed, a beating machine is immediately adopted to conduct one-time full-coverage even rolling on the whole-field rice stubble, physical pressure is applied through a rolling shaft of the beating machine to integrally press down and flatten the upright rice stubble, and the whole-field rice stubble is evenly stressed; the field soil moisture is regulated and controlled according to the axillary bud germination process of the rice stump base and the growth requirements of regeneration season rice plants; corresponding fertilizers are dressed in different growth stages of the regeneration season rice plants, and comprehensive field management is carried out. The rice stumps in the whole field are uniformly rolled after harvesting in the first season, the problems that seedlings emerge irregularly in the regeneration season due to mechanical rolling and spikes are small and few in grain due to high stump remaining are effectively solved, the uniformity is remarkably improved, large spike formation is promoted, and the method has the comprehensive advantages of being easy and convenient to operate, low in cost and easy to popularize.

The invention relates to the technical field of fresh cut flower cultivation, and provides a method for promoting growth of axillary buds of cut-flower roses by applying potassium ions and calcium ions with proper concentrations, which comprises the following steps: applying a fertilizer with the potassium ion concentration of 1.5-3.0 mmol / L and the calcium ion concentration of 3.0-4.5 mmol / L after pruning the cut-flower roses; according to the method for promoting the growth of the axillary buds of the cut-flower roses, provided by the invention, by applying potassium and calcium ions with proper concentration, on one hand, the dormancy of the axillary buds is relieved, and after the potassium and calcium ions with proper concentration are applied, the axillary buds start to grow quickly, so that the harvesting time of the next crop of flowers is obviously advanced; on the other hand, the height of the plants is increased within the same growth time, and therefore the quality of the cut-flower roses is improved.

The application discloses a direct organogenesis genetic transformation method based on spinach stem tips and axillary buds, and relates to the technical field of plant genetic transformation. pCAMBIA1305-GUS As a plant expression vector, the Agrobacterium GV3101 (pSoup-p19) is used as a receptor Agrobacterium to establish a stable genetic transformation system of spinach. The application utilizes the regeneration capacity of the spinach stem tip meristem and axillary bud meristem, can improve the regeneration efficiency and transformation efficiency, and significantly shortens the genetic transformation cycle. The system can be used for the function research of key regulation genes of agronomic traits of spinach and the cultivation of excellent spinach varieties.

The invention belongs to the technical field of plant tissue culture, and discloses a method for cultivating detoxified tissue culture seedlings of trichosanthes kirilowii maxim and the detoxified tissue culture seedlings obtained by the method. The method comprises the following steps: taking a terminal bud or an axillary bud of a trichosanthes kirilowii Maxim plant, performing surface disinfection, and stripping a 0.3-0.5 mm stem tip under a sterile condition to serve as an explant; inoculating the stem tips into a primary culture medium for induction culture, wherein the primary culture medium is an MS culture medium added with 1.0 mg / L 6-BA and 0.2 mg / L NAA; after the adventitious buds germinate, sequentially performing multiplication culture, subculture and rooting culture to obtain complete tissue culture seedlings; and finally carrying out acclimatization and transplantation. According to the method, a systematic and efficient stem tip detoxification culture system is established for trichosanthes kirilowii maxim for the first time, and by optimizing explant selection, disinfection procedures, a key culture medium formula and culture conditions, rapid propagation is achieved, and meanwhile the survival rate of the trichosanthes kirilowii maxim is increased. Various viruses such as cucurbit light mottle mosaic virus (CuMMV) and zucchini green mottle mosaic virus (ZGMMV) carried in a trichosanthes kirilowii Maxim plant can be efficiently removed, and the detoxification rate can reach 96% or above.

The invention discloses an establishment method of a hippuris vulgaris callus regeneration system, and belongs to the technical field of plant tissue culture. The method comprises the following steps: (1) selecting healthy stem segments of hippuris hispida with axillary buds, cleaning the healthy stem segments, and sequentially carrying out surface sterilization on the healthy stem segments by adopting 75% ethanol and a 0.1% mercuric chloride solution; (2) inoculating the sterilized explant into a callus induction culture medium, and inducing to generate a callus; (3) transferring the callus to a proliferation culture medium with the same formula for amplification; (4) transferring the proliferated calluses into an adventitious bud differentiation culture medium to induce adventitious buds; (5) separating the adventitious buds and inoculating the adventitious buds into a rooting and seedling strengthening culture medium to promote rooting and seedling strengthening; and (6) hardening the rooted seedlings and then transplanting the rooted seedlings. The method disclosed by the invention has the advantages of simple culture process, high proliferation efficiency, short period and the like, is suitable for large-scale production of aseptic seedlings, and can provide an effective technical platform for researches such as aquatic ecological restoration, medicine feed raw material supply, subsequent genetic transformation, germplasm preservation and the like.

The application discloses application of an OsDrAp1 gene in regulating axillary bud germination of rices of the same stem, and belongs to the technical field of biotechnology.The nucleotide sequence of the OsDrAp1 gene is shown as SEQ ID NO.1, the germination of the regenerated buds of knockout and overexpression materials of the OsDrAp1 is observed, it is found that the germination rate of the knockout plants is significantly improved, and the germination rate of the overexpression materials is significantly reduced, it is proved that the OsDrAp1 negatively regulates the axillary bud germination of the rices of the same stem, and the OsDrAp1 can be used in breeding research of the rices of the same stem.

The invention belongs to the technical field of plant asexual propagation, and relates to a method for rapid propagation by utilizing triarrhena lutarioriparia stem tip tissue culture. The method comprises the following steps: (S1) cultivating triarrhena lutarioriparia stem segments with axillary buds into seedlings through cuttage, taking stem tips, and disinfecting the stem tips with a botanical disinfectant to obtain triarrhena lutarioriparia stem tip explants; (S2) inoculating the triarrhena lutarioriparia stem tip explant into a callus induction culture medium containing 10-20mg / L of tea polyphenol, and inducing to form a callus; (S3) cutting the callus, inoculating the cut callus to a differential medium containing 0.5-0.8 g / L of proline and 3-5g / L of trehalose, and differentiating to obtain triarrhena lutarioriparia differentiated seedlings; (S4) transferring the triarrhena lutarioriparia differentiated seedlings into a rooting culture medium to induce rooting, so as to obtain triarrhena lutarioriparia stem tip tissue culture seedlings. According to the method, the stem tips of the cutting seedlings serve as explants, a specific disinfection process and an optimized culture medium are comprehensively adopted, a stable and efficient triarrhena lutarioriparia rapid propagation system is constructed, the problems that traditional explants are limited by seasons, the seedling rate is low and the like are solved, and technical support is provided for industrial propagation of triarrhena lutarioriparia.