Tissue culture and tachytelic propagating method for alpine rhododendron
A technique of tissue culture and Rhododendron, which is applied in the field of tissue culture and rapid propagation of alpine Rhododendron to achieve the effect of fast growth, bright and pure flower color, and good branching
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[0040] The species for breeding is the pink variety of alpine Rhododendron, named "Rocket", which originated from Bremen, Germany.
[0041] Adopt the test-tube seedling artificial planting seedling raising method of tender stem segment axillary bud culture method and " two-step method ", it comprises the following steps:
[0042] 1. Adopt the culture method of tender stem section axillary bud:
[0043] (1) Preparation of explants
[0044] a. Plant the imported seedlings in plastic flower pots for maintenance, and cut off the top part of the branches after survival to promote branch growth;
[0045] b. When the newly pumped branches grow to 5-7 cm, and the leaves just turn green and flatten out, cut off the branches, remove the leaves, rinse with tap water for 15 minutes, wash twice with detergent and dry;
[0046] c. Sterilize with 75% alcohol for 1 minute on the ultra-clean bench in the sterile room; sterilize with 1:50 Xinjieer liquid for 20 minutes; then sterilize with 0....
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