Tissue culture and tachytelic propagating method for alpine rhododendron

A technique of tissue culture and Rhododendron, which is applied in the field of tissue culture and rapid propagation of alpine Rhododendron to achieve the effect of fast growth, bright and pure flower color, and good branching

Inactive Publication Date: 2005-01-26
上海市闵行区农业科学研究所
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  • Abstract
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Problems solved by technology

Alpine Rhododendron usually has no seeds and mainly propagates by cuttings, which are affected by...

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0040] The species for breeding is the pink variety of alpine Rhododendron, named "Rocket", which originated from Bremen, Germany.

[0041] Adopt the test-tube seedling artificial planting seedling raising method of tender stem segment axillary bud culture method and " two-step method ", it comprises the following steps:

[0042] 1. Adopt the culture method of tender stem section axillary bud:

[0043] (1) Preparation of explants

[0044] a. Plant the imported seedlings in plastic flower pots for maintenance, and cut off the top part of the branches after survival to promote branch growth;

[0045] b. When the newly pumped branches grow to 5-7 cm, and the leaves just turn green and flatten out, cut off the branches, remove the leaves, rinse with tap water for 15 minutes, wash twice with detergent and dry;

[0046] c. Sterilize with 75% alcohol for 1 minute on the ultra-clean bench in the sterile room; sterilize with 1:50 Xinjieer liquid for 20 minutes; then sterilize with 0....

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Abstract

The invention relates to alpine rhododendron tissue culture quick-propagation method. All the medium is prepared according to following material: medium for inducing explant: 1/4MS, MS ferric salt, VitB50.2mg, lactalbumin hydrolysate 300mg, sucrose 30g+Kt1.0mg/L; medium for propagating cluster sprout: 1/2MS, Kt0.5mg/L; medium for rooting: 1/2MS, NAA5mg/L, active carbon 0.5%. The method of alpine rhododendron tissue culture quick-propagation is characterized as test tube sprout provisional planting method adopting tender stem segment axillary bud culture method and 'two-step' method. The advantages of the invention lie in: the growth is not influenced by season, plant has strong resistance to stress, and the plant has big colorful flowers.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method of rhododendron alpine, belonging to the technical field of flower cultivation. Background technique [0002] Alpine Rhododendron (Rhododendron radinum) is a high-grade flower plant of Rhododendron genus shrubs in the family Rhododendronaceae. It is native to the alpine dense forests in the central and western regions of my country. There are many kinds of flower colors such as red, pink, purple, yellow, white, etc., which are gorgeous and luxurious, and are deeply loved by people. Alpine Rhododendron usually has no seeds and is mainly propagated by cuttings, which are affected by the material of the mother plant and the breeding season, and often cannot meet the needs of the market. Contents of the invention [0003] The purpose of the present invention is to provide a method for rapid propagation of rhododendron alpine tissue culture which has a fast propagation speed and is not aff...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 汤桂钧
Owner 上海市闵行区农业科学研究所
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