1215 results about "Sucrose" patented technology

The invention relates to a preparation method and application of a carbon dot-based nano enzyme. The preparation method of the carbon dot-based nano-enzyme comprises the following steps: taking sucralose and B vitamins as raw materials, and preparing the carbon dot-based nano-enzyme through a one-pot hydrothermal method. The preparation method of the carbon dot-based nano-enzyme provided by the invention is simpler in catalytic condition and higher in safety. The carbon dot-based nano-enzyme prepared by the invention has the advantages of high oxidase-like activity, low drug resistance, high biocompatibility and the like, can inhibit multi-drug-resistant bacteria, and can be applied to the aspects of aquatic product preservation and the like.

The invention relates to the technical field of food processing, and in particular relates to a sparrow chensinensis Kangpu tea as well as a preparation method and application thereof, the preparation method comprises the following steps: extracting sparrow chensinensis and water according to a ratio of 1: (50-100) at 70-90 DEG C for 40-60 minutes, filtering, and adding cane sugar to obtain a fermentation substrate; the preparation method comprises the following steps: preparing seed liquid, namely activating casei paracasei, phytobacterium plantarum, acetobacter pasteurianus and kluyveromyces marxianus into seed liquid, inoculating the seed liquid into a substrate according to the inoculum size of 0.5-1.5%, fermenting for 5-10 days under the conditions that the temperature is 37 DEG C and the pressure is 0.03-0.05 MPa, and sterilizing (optionally drying) to obtain the parochetus ocellatus Kangpu tea. The preparation method of the parochetus ocellatus Kangpu tea is accurate and controllable in fermentation, safe and stable; the product retains the nutrition of the raw materials, the bioavailability of polyphenol after fermentation is improved, the antioxidant activity is enhanced, the flavor is soft, caffeine is avoided, and the product is suitable for a wide range of people and can be used for preparing antioxidant functional food.

The invention relates to the technical field of microorganisms, in particular to bifidobacterium bifidum BGLP1 capable of regulating sugar digestion and producing GLP-1 at high yield and application of the bifidobacterium bifidum BGLP1. The preservation number of the bifidobacterium bifidum BGLP1 is CGMCC (China General Microbiological Culture Collection Center) No.35718, and the bifidobacterium bifidum BGLP1 can be used for preparing the feed additive. The strain has the capability of inhibiting alpha-glucosidase and alpha-amylase; sTC-1 cells are stimulated to secrete GLP-1, and the blood glucose reducing pathway capacity of the intestinal tract is activated; the activity of pancreatic lipase is obviously inhibited, and the dietary fat absorption capability can be effectively reduced; redundant cane sugar is converted into exopolysaccharide, so that the probiotic function capability is improved; the capability of converting white fat into brown fat is promoted; macrophages are activated, and the body immune function is improved. In addition, the bifidobacterium bifidum BGLP1 also has relatively strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body.

The invention discloses a preparation method of high-viscosity cationic starch, which belongs to the technical field of modified starch, and comprises the following steps: crosslinking epichlorohydrin, ball-milling and cationizing; the epoxy chloropropane crosslinking comprises the following steps: mixing starch, calcium hydroxide and deionized water, stirring at 40-50 DEG C, adding sodium stearoyl lactylate, stirring at 50-55 DEG C, naturally cooling to room temperature, adding sodium chloride and epoxy chloropropane, stirring, dropwise adding a potassium hydroxide aqueous solution, stirring, adjusting the pH value to 6, filtering, taking filter residues, washing and drying to obtain crosslinked starch; in the step of ball milling, calcium hydroxide, sucrose fatty acid ester SE-11 and deionized water are mixed and then subjected to ball milling, and a ball-milled material is obtained; the prepared high-viscosity cationic starch is high in paste liquid viscosity and cationic substitution degree, good in paste liquid viscosity stability, uniform in substitution degree and paste liquid viscosity, high in reaction efficiency in cationization and low in alkali consumption.

The invention belongs to the technical field of gel. The invention provides high-strength starch sodium octenylsuccinate gel and a preparation method thereof.The preparation method comprises the steps that S1, corn and cassava starch are mixed according to the mass ratio of 1: (4.4-4.6) to obtain a mixture, and then the mixture and soft water are dispersed according to the mass ratio of 1: 1.1 to obtain starch milk; s2, adjusting the pH value of the starch milk to 9.0 by using a sodium hydroxide solution, then adding octenyl succinic anhydride at 25 DEG C, then adding sucrose ester and pectin, neutralizing the pH value to 4.5 by using an HC1 solution, and carrying out suction filtration, washing and crushing to obtain starch sodium octenyl succinate powder; and S3, gelatinizing the starch sodium octenylsuccinate powder, and storing the gelatinized starch sodium octenylsuccinate powder at low temperature to obtain the high-strength starch sodium octenylsuccinate gel. According to the invention, by reducing the ratio of corn starch to cassava starch, the viscosity of the prepared starch sodium octenylsuccinate is further improved while a lower gelatinization temperature is ensured.

The invention relates to the technical field of functional food processing, and discloses magnesium-rich gel soft sweets and a preparation method thereof. The jelly sweets comprise the following components in parts by weight: 3 to 15 weight percent of first gelling agent, 0.2 to 2 weight percent of second gelling agent, 20 to 50 weight percent of dietary fiber raw material, 10 to 40 weight percent of sugar alcohol raw material, 3 to 10 weight percent of acidity regulator, 0.3 to 1 weight percent of buffer salt, 0.4 to 2 weight percent of essence, 0.4 to 2 weight percent of pigment, 10 to 30 weight percent of mineral raw material and 0 to 0.5 weight percent of anti-sticking oil. The gel soft sweets are rich in magnesium element, and are suitable for people with magnesium deficiency, electrolyte supplementation after exercises and auxiliary conditioning of sleep disorders. The gel soft sweets are good in chewiness and coordinated in sour and sweet taste, sugar alcohol is partially replaced with low-GI dietary fibers, calorie intake is reduced, satiety is enhanced, intestinal health is supported, the taste is elastic, high-power sweeteners of sucralose and stevioside are not added, and the gel soft sweets are easily accepted by mass consumers.

The invention discloses stem cell freeze-dried powder for promoting skin healing and a preparation method of the stem cell freeze-dried powder. The freeze-dried powder comprises an effective component, a freeze-drying protective agent group and a polymer protective agent group, the effective component is umbilical cord mesenchymal stem cell conditioned medium freeze-dried supernatant, the protective agent group is selected from one or more of cane sugar, trehalose, lactose and mannitol, and the polymer protective agent group is selected from one or more of polyvinylpyrrolidone (PVP) and glucan. The preparation method comprises the following steps: collection of a conditioned culture medium, preparation of a freeze-drying protective agent solution, mixing treatment, low-temperature pre-freezing and freeze-drying, collection and packaging of freeze-dried powder and the like. The method can effectively retain stem cell-derived active factors and enhance the stability and redissolvability of the freeze-dried powder, and is suitable for skin wound repair, regenerative medicine and other scenes.

The invention belongs to the technical field of antibacterial polyurethane, and particularly relates to a preparation method of antibacterial polyurethane foam. The preparation method comprises the following steps: (1) adding 0.5-1 part by weight of water, 1-3 parts by weight of a catalyst, 1-6 parts by weight of a foam stabilizer and 0.5-1 part by weight of an inorganic antibacterial filler into 100 parts by weight of antibacterial polyether under the condition of 25-35 DEG C to obtain a combined material; (2) adding 100-110 parts by weight of polyisocyanate into the combined material obtained in the step (1) under the condition of 25-35 DEG C, stirring, foaming and curing to obtain polyurethane foam; the number-average molecular weight of the antibacterial polyether is 550-650, guanidine hydrochloride, cane sugar, diethylene glycol and ethidene diamine are used as initiators and react with epoxypropane to prepare the antibacterial polyether, and the mass fraction of epoxypropane in the antibacterial polyether is 75-80%. The foam prepared by the method is high in strength and high in antibacterial property.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

The invention provides a sore-throat relieving and detoxification granule and a preparation method thereof, and belongs to the technical field of medicines, the sore-throat relieving and detoxification granule comprises the following components: mint volatile oil, beta-cyclodextrin, a sore-throat relieving and detoxification extract, a filler and sucralose, meanwhile, the mint volatile oil and the beta-cyclodextrin are prepared into an inclusion compound, the inclusion compound and other raw materials are mixed and granulated, and the sore-throat relieving and detoxification granule is obtained. According to the sore-throat-relieving and detoxifying granules and the preparation method thereof, the stability of mint volatile oil in the sore-throat-relieving and detoxifying granules is improved, and the antipyretic and anti-inflammatory effects of the sore-throat-relieving and detoxifying granules are enhanced.

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

The invention relates to polygonatum cyrtonema polysaccharide and application thereof. The novel polysaccharide PRP is extracted and purified from polygonatum cyrtonema and is neutral inulin type polysaccharide. The PRP takes a node alpha-D-glucose-(22)-beta-D-fructose similar to cane sugar as a center. The C6 site of glucose has an additional beta-fructose (2-> 6) substituent, and two chains extend out of the core through a (2-> 1)-beta-fructose bond to form a Y-type moderate branch structure. Compared with other selenium nanoparticles with stable polysaccharides, the selenium nanoparticles coated with polygonatum polysaccharide provided by the invention have smaller initial particle size, more negative zeta potential, stable batch repeatability and excellent long-term stability.

The invention provides a sweet substance screening method and application, and relates to the field of sweet substance development and application, and the sweet substance screening method is characterized by comprising the following steps: providing a sweet substance, analyzing the sweet substance by adopting an electronic tongue technology and a molecular docking technology to obtain first information and second information of the sweet substance, and constructing a bimodal model at the same time, obtaining a screening result; according to the technical scheme provided by the invention, taste features of various common sweet substances are analyzed in a mode of combining an electronic tongue technology and a molecular docking technology, so that taste feature differences of the sweet substances and sucrose can be effectively compared; a machine learning prediction model based on taste attribute values of nine electronic tongue sweet substances and four molecular docking characteristic values is established, and sensory evaluation is used for verification; sweet substances similar to cane sugar in taste are rapidly screened, and the screening accuracy is improved. Based on the method, a thought is provided for excavation and development of novel sweet substances.

The invention relates to the field of vegetable preservation, and discloses a preparation method of a volatile oil preservative and application of the volatile oil preservative in artemisia selengensis preservation, and the method comprises the following steps: step 1, weighing waste leaves and stems of artemisia selengensis, adding a sodium chloride solution, crushing with a juicer, then transferring into a round-bottom flask, enabling the round-bottom flask to be hermetically connected with a volatile oil steam distillation device to extract artemisia selengensis volatile oil, receiving volatile oil by using a centrifugal tube, and transferring the upper layer artemisia selengensis volatile oil by using a pipette; and step 2, weighing a hydrophilic emulsifier sucrose ester and chitosan powder, slowly adding the hydrophilic emulsifier sucrose ester and chitosan powder into the acetic acid solution, stirring while adding to avoid agglomeration, stirring at room temperature until the hydrophilic emulsifier sucrose ester and chitosan powder are completely dissolved to form a transparent or slightly milky white solution, adding the extracted artemisia selengensis volatile oil and glycerol, stirring in a sealed manner at room temperature, and degassing to obtain the artemisia selengensis volatile oil preservative. According to the method, a closed loop of waste recycling, preservative and homologous vegetable preservation can be realized, and the shelf life of artemisia selengensis is prolonged; meanwhile, the artemisia selengensis volatile oil has a certain compensation effect on the loss of the flavor of the clean artemisia selengensis.

The invention discloses preparation and application of a rabies virus G protein mRNA vaccine freeze-drying preparation, and relates to the field of veterinary biological products. According to the invention, rabies virus G protein mRNA is prepared on a large scale through an in-vitro transcription technology, and an mRNA-LNP (lipid nanoparticle) delivery system is successfully constructed by adopting a microfluidic encapsulation process. Through freeze-drying protective agent formula screening (including cane sugar, trehalose and mannitol) and freeze-drying procedure parameter optimization (including pre-freezing temperature, main drying rate and secondary drying residual moisture control), the freeze-dried mRNA vaccine preparation which can stably exist under the condition of 4 DEG C is finally obtained.

The invention discloses a yak milk casein ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and relates to the technical field of medicines and health-care products. The preparation method of the yak milk casein ACE inhibitory peptide comprises the following steps: taking yak milk casein as a substrate, adding NaCl and cane sugar to prepare a fermentation culture medium, inoculating a high-yield protease strain into the fermentation culture medium for constant-temperature fermentation, deactivating enzyme after fermentation, centrifugally collecting supernate, and freeze-drying to obtain the yak milk casein ACE inhibitory peptide. The yak milk casein ACE inhibitory peptide oral liquid contains sodium carboxymethyl cellulose, cane sugar, citric acid and yak milk casein ACE inhibitory peptide, the invention also provides an application of the yak milk casein ACE inhibitory peptide in preparation of drugs or health-care products for assisting in lowering blood pressure. The yak milk casein ACE inhibitory peptide disclosed by the invention has an antihypertensive function and good palatability, fills the blank of a yak milk deep processing product market, and provides a technical path for extension of yak milk from a traditional dairy product to the biological medicine field.

The invention relates to the technical field of beverages, and particularly discloses a high-protein beverage containing psicose and a preparation method of the high-protein beverage. The high-protein beverage containing psicose is prepared from the following raw materials in percentage by mass: 13 to 17 percent of supplementary protein, 4 to 5 percent of an emulsifier, 3.10 to 3.14 percent of a flavoring agent, 0.02 to 0.06 percent of a thickening agent, 0.005 to 0.015 percent of a stabilizer, 0.008 to 0.01 percent of a coloring agent, 0.04 to 0.08 percent of an enzyme preparation and the balance of water, the flavoring agent is prepared from D-psicose, erythritol, sucralose and stevioside. The D-psicose in the flavoring agent is extremely low in calorie, does not cause increase of blood sugar and insulin, and has a unique taste, and the erythritol, the sucralose and the stevioside are matched, so that the taste of sucrose is reduced on the premise of low calorie, and the problem of bitter taste of a sugar substitute beverage is solved, so that the flavor of the protein beverage is improved.

The invention belongs to the technical field of seasoning processing, and particularly discloses salad dressing capable of improving the stability of natural pigments and a preparation method of the salad dressing. The salad dressing capable of improving the stability of the natural pigment comprises the following raw materials: high fructose corn syrup, yolk liquid, white granulated sugar, potassium sorbate, lemon concentrated juice, table vinegar, water, glycerol, the natural pigment, titanium dioxide, sucrose fatty acid ester, edible salt, xanthan gum, soybean oil, acetylated distarch adipate, sodium ascorbate, mustard powder and a rosemary extract. The HLB (Hydrophile Lipophile Balance) value of the sucrose fatty acid ester is The synergistic effect of all the components is remarkable, multiple defense lines of oxidation resistance, system stability, corrosion prevention and the like are constructed, and the probability of natural pigment fading in the salad dressing storage process is reduced.

The invention discloses a preparation method and application of lithium iron phosphate, and belongs to the field of lithium ion battery positive electrode materials. The preparation method comprises the following steps: (1) pre-sintering a lithium iron phosphate precursor; (2) uniformly mixing the pre-sintered lithium iron phosphate precursor with a lithium source, a phosphorus source, a doping element and a carbon source, grinding, and carrying out spray drying to obtain a spray material; and (3) carrying out sintering treatment on the spray material, and carrying out crushing and demagnetizing treatment after cooling to obtain the lithium iron phosphate. The pre-sintering temperature in the step (1) is 500-750 DEG C, and the pre-sintering time is 4-8 hours. The lithium source in the step (2) is one or more of lithium carbonate, lithium hydroxide, lithium dihydrogen phosphate and lithium phosphate; the phosphorus source is one or more of phosphoric acid, lithium dihydrogen phosphate and iron phosphate; the carbon source is at least two of glucose, cane sugar, polyethylene glycol and starch. The lithium iron phosphate provided by the invention can achieve the effects of low specific surface area, high compaction density and excellent electrochemical performance.

The invention provides a teak camel moth artificial feeding feed and a teak camel moth artificial feeding method, the feed comprises the following components by weight: 8% of wheat germ powder, 4% of soybean meal, 4% of yeast powder, 2% of casein, 2% of sucrose, 1.8% of agar powder, 1.5% of cellulose, 0.8% of Webster salt, 0.6% of vitamin C, 0.2% of sorbic acid, 0.3% of methyl p-hydroxybenzoate, 0.1% of cholesterol, 0.1% of choline chloride, 0.05% of inositol, 0.01% of folic acid, and the balance of water. And the balance of deionized water. The invention further provides an artificial feeding method of teak camelmoths, and the artificial feeding method comprises the steps of 1, adult mating and egg laying, 2, egg disinfection and incubation and 3, larva rearing. The feed does not contain host material teak leaves, and full-artificial continuous large-scale feeding of the teak camelmoths can be achieved.

The application relates to the technical field of cough-relieving medicines, and discloses a high-efficiency cough-relieving loquat particle composition which is composed of the following components in parts by weight: loquat leaf extract 30-40 parts; white front extract 10-15 parts; platycodon grandiflorum extract 6-10 parts; white mulberry bark extract 18-25 parts; stemona sessiliflora extract 7-10 parts; menthol 0.1-0.5 part; polyethylene glycol 2-5 parts; mannitol 8-12 parts; ethyl cellulose 3-6 parts; hydroxypropyl methyl cellulose 1-3 parts; phosphatidylcholine 1-3 parts; polysorbate 80 1-3 parts; cross-linked sodium carboxymethyl cellulose 4-6 parts; and sucrose 40-60 parts. The double dispersion system combining the immediate-release component and the sustained-release component is adopted, the solubility and the initial release speed of the traditional Chinese medicine extract are remarkably improved through the addition of polyethylene glycol and mannitol, and the technical effect that the drug effect appearing time is remarkably shortened is achieved. Compared with the technical scheme relying on only a single release mode in the prior art, the deficiency that the drug effect is slow and the symptoms of the patient cannot be timely relieved is solved.

The invention provides a method for detecting related substances of sucrose octasulfate salt auxiliary materials, and particularly relates to the field of medicines. An SCX cation exchange chromatography filler and a C18 filler are mixed according to the ratio of (0.8-1.2): (3-5) to serve as a filling agent; the specification of a chromatographic column is CAPCELLPAK CR C18 + SCX 5 [mu] m, 250 * 4.6 mml.D.; 18-22 mM / L ammonium formate and 0.08-0.12% formic acid are used as a mobile phase A, and acetonitrile is used as a mobile phase B; a detector is CAD, the spray temperature is High, the collection frequency half-peak width is 0.1-0.2 min, the column temperature is 28-32 DEG C, the flow rate is 0.8-1.2 ml / min, and the sample injection volume is 4-6 [mu] l; wherein acetonitrile and water in a ratio of (3-5): (5-7) are used as diluents, and gradient elution is carried out; the method provided by the invention can effectively separate and accurately analyze each impurity, has strong specificity and good separation degree, and can effectively control the product quality.

The invention belongs to the technical field of solenopsis invicta prevention and control, and particularly relates to solenopsis invicta food bait as well as a preparation method and application thereof. The invention provides red imported fire ant food bait. The red imported fire ant food bait is prepared from energy main materials and auxiliary materials, the energy main material consists of the following components in percentage by weight: 50-70% of insect protein powder, 5-20% of corn flour, 9-11% of peanut oil, 9-11% of fish meal and 9-11% of cane sugar. The invention also provides an attractant matched with the red imported fire ant food bait. According to the invention, the efficiency of finding bait by the red imported fire ants can be improved, meanwhile, the recruitment behaviors of the worker ants are further increased, more worker ants are lured, and the comprehensive trapping effect is good.

Hair Styling Compositions and Processes: The disclosure relates to compositions comprising (a) at least one sucrose ester; (b) at least one nonionic polysaccharide thickening agent; (c) at least one cationic surfactant; (d) at least one emulsifier selected from fatty alcohols, esters, or a combination thereof; (e) optionally, at least one C3-C8 polyol selected from diols and triols; and (f) optionally, at least one fatty compound. The compositions may be used as leave-in hair styling compositions. The disclosure also relates to kits comprising the compositions and processes for using the compositions. Figure for the abstract: Figure 1

A liquid composition containing, as a sucrose fatty acid ester in liquid form, a sucrose fatty acid ester in which the constituent fatty acid has 16 or more carbon atoms, the liquid composition comprising terpenes and a monohydric alcohol and / or a dihydric alcohol, wherein the content of the terpenes is 0.08-1.2 parts by weight relative to 1 part by weight of the sucrose fatty acid ester, the content of the monohydric alcohol and / or the dihydric alcohol is 0.50-5.0 parts by weight relative to 1 part by weight of the sucrose fatty acid ester, and the content of the sucrose fatty acid ester is 15-80% by weight of the total amount of the liquid composition.

The method comprises the following steps: (1) peeling seeds, disinfecting the peeled seeds with 75% ethanol and 10% NaClO, and inoculating the disinfected seeds to a hormone-free MS culture medium; (2) after the seeds germinate, cutting a hypocotyl which grows robustly, and cutting the hypocotyl into sections of 1cm as explants; (3) inoculating the hypocotyl to an induction medium (MS + 1.5 mg / L 6-BA + 1.5 mg / L 2, 4-D + 0.5 mg / L NAA + 30 g / L cane sugar + 7 g / L agar with pH of 6.2), and culturing for 30 days under the conditions of 25 + / -2 DEG C and illumination for 12 h / d to obtain a white green callus with a good state, and the induction rate reaches 71.1%; and (4) transferring the callus to a proliferation culture medium (MS + 1.5 mg / L 6-BA + 0.5 mg / L NAA + 30g / L sucrose + 7g / L agar, pH being 6.2), and continuously culturing for 30 days to realize expansion proliferation of the callus. The method is easy and convenient to operate and good in repeatability, and an effective way is provided for rapid propagation, germplasm preservation and secondary metabolite production of the rheum himalayana.

The invention discloses an embedding method for stabilizing and slowly releasing beta-carotene. The embedding method comprises the following steps: selecting soybean oil; the stirring conditions are as follows: 300 r / min and 75 DEG C; preparing a water phase: stirring 80 kg of maltodextrin and 20 kg of cane sugar at 50 DEG C until 200 kg of distilled water is completely dissolved; the homogenizing conditions are as follows: the high-speed mixing and shearing emulsifying machine shears for 5 minutes at the speed of 17000 r / min, and the high-pressure homogenizing machine homogenizes for 2 minutes at the pressure of 20 MPa; the spray drying conditions are as follows: the pressure is 0.5 MPa, the inlet temperature is 170 DEG C, and the flow rate is 10 mL / min. The particle size D of the optimized beta-carotene microcapsule product is 95lt; according to the present invention, the prepared nano-particles have characteristics of uniform particle size, 300 nm, embedding rate of 94.2%, good stability in the 90-day storage period, and significantly improved solubility.

The invention discloses a method for improving the biomass and flavone content of a saussurea involucrata cell culture, which comprises the following steps of: culturing yellow-white saussurea involucrata callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS, cane sugar, naphthylacetic acid, 6-benzyladenine, coconut extract and agar in a container, adding distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the biomass of the saussurea involucrata callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.

An object of the present invention is to provide a recombinant yeast that can highly express a target protein in a tightly regulated manner without using methanol, and to provide a method for producing a target protein using the recombinant yeast, a kit for producing the recombinant yeast, and a method for producing the recombinant yeast. According to the present invention, there is provided a recombinant yeast containing a first exogenous sucrose-inducible promoter for expressing a target protein.

The invention discloses application of 4-isopropyltoluene to improvement of depression-like behaviors of mice. An experiment adopts a chronic constraint stress method to construct a depression model, through behavioral detection such as a sucrose preference test, a tail suspension test and a novel inhibition ingestion test, and in combination with molecular biology means such as immunofluorescent staining and Western blot, the anti-depression effect and potential mechanism of 4-isopropyltoluene are systematically evaluated, and the anti-depression effect and potential mechanism of 4-isopropyltoluene are evaluated. Results show that 4-isopropyltoluene plays a significant anti-depression role by inhibiting neuroinflammation, enhancing glial cell support function and activating AKT signal pathway, and has the potential of being developed into a novel anti-depression candidate drug.