57results about "Esterified saccharide compounds" patented technology

The present application relates to a kind of efficient active separation and purification method and purposes of elaeagnus conferta polyphenol, comprising: elaeagnus conferta powder is extracted with ethanol solution, after ultrasonic extraction, centrifugal supernatant is obtained, repeat multiple times after being combined, concentrated by rotary evaporation, and obtain elaeagnus conferta polyphenol concentrated extract;Elaeagnus conferta polyphenol concentrated extract is centrifuged, and the supernatant is used as chromatography sample liquid;Chromatography sample liquid is added to Flash liquid chromatography by liquid sample loading mode, is separated by gradient elution mode to chromatographic column, and each elution component is collected in turn, and concentrated respectively;The concentrated solution of each elution component is added to chromatographic column again to carry out second gradient elution separation, and chromatographic column separation elution is repeated three times, and three kinds of purified elaeagnus conferta polyphenol components are obtained after freeze drying.The present application realizes the high-resolution fractionation elution of polyphenol monomer with high structural similarity by the synergistic regulation of mobile phase polarity gradient and elaeagnus conferta polyphenol molecular hydrophobic interaction.

The present invention provides a method of isolating arjunetin from the bark of Terminalia arjuna. The invention provides a simplified, cost-effective method and scalable method for isolation of arjunetin from the Terminalia bark. The present invention encompasses an ethanolic extract of arjunetin that comprises arjunetin with 95% purity. The ethanolic extract of arjunetin is obtained from solvent extraction without using column chromatography. Arjunetin has various therapeutic applications, anti-diabetic, anti-inflammatory, anti-cancer, and anti-viral drugs.

The disclosure belongs to the technical field of organic synthesis and discloses a glycosylation and alkylation method involving a benziodoxole reagent. In the glycosylation method or alkylation method, a raw material (glycosyl donor or alkylation reagent) under a combined action of the benziodoxole reagent and an acid catalyst are activated and then reacted with a nucleophilic reagent to obtain a corresponding glycosylation product or alkylation product. In the method of the disclosure, by introducing the benziodoxole reagent and working in combination with an acid catalyst, efficient reaction is achieved without the need to use a precious metal heavy metal catalyst.

The application discloses a selenoglycoside compound and a synthesis method, and aims at a series of technical defects in the existing selenoglycoside synthesis technology, such as high reagent toxicity, strong irritability, poor stability, harsh reaction conditions, complicated operation, insufficient stereoselectivity, poor substrate universality and difficulty in industrial application, and provides a green and efficient selenoglycoside compound synthesis method with strong universality, which has remarkable beneficial technical effects. The core reaction substrate adopted in the application is a glycosyl selenosulfonate and a (hetero) aryl boronic acid, both of which are conventional reagents easy to obtain, have no irritating odor, and have excellent chemical stability and environmental tolerance, thereby avoiding the use of high-toxicity, high-irritability and low-stability reagents such as diselenide, selenol and glycosyl stannane in the traditional synthesis process from the source, greatly reducing the safety control cost and three-waste treatment pressure in the synthesis process, and meeting the development requirements of green chemistry.

The application discloses an enzyme method for synthesizing unnatural sugar and application thereof, and belongs to the technical field of organic chemical synthesis. The unnatural sugar comprises an unnatural sugar obtained by introducing an acyl group into a hydroxyl group at the 6th position of a bare sugar through an acylation reaction; and the bare sugar is obtained by performing derivatization modification on an amide site of a natural sugar. The synthesis method uses a SubC enzyme as a catalyst to realize selective acylation of the hydroxyl group at the 6th position of the bare sugar, and synthesizes the unnatural sugar with an ideal yield. The unnatural sugar can be applied to glycomics metabolic labeling and mass spectrometry analysis as a marker, has excellent biological safety, specificity and labeling efficiency, can avoid non-specific labeling, and has no obvious cytotoxicity.

The present invention relates to a process of separating an acidic sophorolipid, or a lactonic sophorolipid, especially an acidic sophorolipid enriched with acetylated acidic sophorolipid from a lactonic / acidic sophorolipid-containing product and the acidic sophorolipid, especially the acid-ic sophorolipid enriched with acetylated acidic sophorolipid obtained by said process.

The invention provides fluoroalkoxyalkylene dihydroimidazo[5,1-d]tetrazinone compounds and related compounds, pharmaceutical compositions, and their use in treating cancer.

The application discloses a full synthesis method of fortimicin B and derivatives thereof, and specifically, the method comprises the following steps: 3-ester group-2-pyrone and N-substituted-2-oxazolone are subjected to asymmetric reverse electron demand Diels-Alder reaction to generate a bridged lactone intermediate, then a series of transformations are performed to obtain an amino cyclic polyol fragment fortimicin B derivative; amino aldehyde and halogenated amino acid ester are subjected to carbon-carbon bond generation reaction mediated by divalent chromium and monovalent cobalt to obtain an amino alcohol intermediate, and subsequent transformation is performed to obtain an amino sugar fragment 6-isomer-erythromycin amine derivative; under the action of a gold catalyst, stereoselective glycosidation reaction occurs between the amino cyclic polyol fragment fortimicin B derivative and the amino sugar fragment 6-isomer-erythromycin amine derivative, ring opening and deprotection steps are performed, and fortimicin B and derivatives thereof are obtained. The synthesis route is short, the operation is simple, raw materials and reagents are easy to obtain, the synthesis route has the advantages of modularity and divergence, and fortimicin B and derivatives thereof which are difficult to synthesize according to the prior art can be synthesized.

The application designs and synthesizes polysaccharide construction monomers (mannose, galactose or glucose) containing azide group and terminal alkyne group at the same time, realizes monosaccharide molecular coupling through copper catalyzed azide-alkyne click chemistry reaction (CuAAC), the rate of copper catalyzed azide-alkyne click chemistry is fast, the yield is high, the product is stable, there is no by-product, the synthesis of polysaccharide molecules composed of single kind of polysaccharide construction monomers and sequence controllable polysaccharide molecules composed of different kinds of polysaccharide construction monomers can be realized. Similarly, the azide-alkyne click chemistry is used for modifying fluorescent molecules at the end of the polysaccharide molecule, and the synthesis of sequence controllable polysaccharide molecules with fluorescent properties is realized.

The present invention relates to the field of modified proteins, immunogenic compositions and vaccines comprising the modified proteins, their manufacture and the use of such compositions in medicine. More particularly, it relates to a modified Sap2 (Secreted Aspartyl Proteinase 2 of Candida albicans') protein. The modified Sap2 protein can be used as a carrier protein for other antigens, particularly saccharide antigens or other antigens lacking T cell epitopes.

The application belongs to the technical field of health food, and particularly relates to a chelated iron donkey-hide gelatin sugar peptide composition and a preparation method thereof. The preparation method comprises the following steps: adding a protease to a gel solution for enzymolysis, performing ultrafiltration grading after the enzymolysis is completed, collecting a concentrated solution, and obtaining a donkey-hide gelatin peptide enzymolysis solution; adding an ethanol solution of a ribose derivative to the donkey-hide gelatin peptide enzymolysis solution for a Maillard reaction, adjusting the pH after the reaction is completed, and obtaining a donkey-hide gelatin peptide solution; adding a ferrous chloride solution to the donkey-hide gelatin peptide solution for a stirring reaction, removing unreacted ferrous chloride by nanofiltration treatment after the reaction is completed, and obtaining modified chelated iron donkey-hide gelatin sugar peptide powder after freeze-drying of the nanofiltration concentrated solution; adding beta-cyclodextrin to a phosphate buffer solution, adding the modified chelated iron donkey-hide gelatin sugar peptide powder and vitamin C, and obtaining the chelated iron donkey-hide gelatin sugar peptide composition through ultrasonic treatment, freeze-drying and grinding. The composition has a high iron content.

New use of combination of extraction of Noni and stevia reveals improvement effects on heart failure in animals.

The present invention belongs to the field of preparation and use of oligosaccharide active substances. Provided are a preparation method for a high-acyl gellan oligosaccharide and the use thereof in immune induction. The preparation method uses composite oxidation hydrolysis technology and uses food-grade and high-purity raw materials, and by means of steps of addition of reagents in specific ratios, strict control on hydrolysis conditions, concentration, impurity removal, and membrane filtration and purification, etc., successfully prepares the high-acyl gellan oligosaccharide with the degree of polymerization of 4-10. Preparing a solution from said oligosaccharide and spraying leaves of Arabidopsis with same can significantly activate the salicylic acid signaling pathway of Arabidopsis and improve the activity of related enzymes in vivo, thereby activating the immune system of the plant, effectively resisting the invasion of pathogenic bacteria, and reducing a disease index. Hence, the high-acyl gellan oligosaccharide is an efficient plant immunity inducer material. The present invention not only provides a novel effective substance for the field of plant immune induction but also develops a corresponding preparation method, thereby exhibiting broad application prospects.

Provided are novel compounds, as well as pharmaceutical compositions, methods of synthesis, and methods of use, that include a polymeric carbohydrate backbone, one or more mannose-binding C-type lectin receptor targeting moieties, and a nitrogen-containing bisphosphonate compound attached to the backbone via thiol-maleimide conjugation. The thiol-maleimide conjugation of the bisphosphonate to the polymeric carbohydrate backbone provides methods of use of the compounds and compositions thereof to release a therapeutic payload upon internalization into mannose-binding C-type lectin receptor expressing cells, such as tumor-associated macrophages (TAMs), for the treatment of various diseases, such as cancer, autoimmune diseases, and inflammatory diseases.

This invention provides a method for extracting active ingredients from *Erigeron breviscapus*, belonging to the field of pharmaceutical technology. Based on the differences in the physicochemical properties of the compounds, this invention first achieves preliminary separation of caffeic acid esters and scutellarin, followed by further purification and separation based on the properties of the two types of substances. Specifically, this invention uses ethanol-water solutions of different concentrations to extract caffeic acid esters stepwise, and uses macroporous and microporous resins for preliminary separation of the caffeic acid esters. Scutellarin is then preliminarily separated through acid-base treatment. Finally, high-purity scutellarin, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and scutellarin are obtained through reversed-phase industrial chromatography. This invention achieves the extraction and separation of multiple active ingredients from *Erigeron breviscapus*, and all solvents used in the extraction and separation process are recyclable, resulting in low cost.

A kind of chestnut burr tannin and its extraction method and application belong to the technical field of natural product extraction.The method is that after the dry chestnut burr is crushed, it is mixed with buffer solution of specific pH value, and biological enzymes such as cellulase and pectinase are added for enzymolysis under mild conditions, the cell wall structure is effectively destroyed, and then inactivation, filtration, vacuum concentration and spray drying are carried out to obtain chestnut burr tannin product.The present application significantly improves the extraction efficiency and product purity of tannin by optimizing the key parameters such as buffer system, type and amount of enzyme, solid-liquid ratio, reaction temperature and time.The whole process condition is mild, which avoids the damage of high temperature and strong acid and alkali to the structure of tannin, reduces the use of organic solvent, and has the advantages of green environmental protection, high efficiency, easy to scale production, etc.The prepared chestnut burr tannin can be used in water treatment, leather making, medicine and food fields, and provides an effective way for the resource high-value utilization of chestnut burr waste.

Disclosed are a device and a method for preparing a sucrose-6-ester. The device includes a tank body, a heating pipe, an annular cooling apparatus, and a motor, wherein the annular cooling device and the heating pipe are arranged in the tank body in nested manner; the annular cooling apparatus includes a condensation inner wall, a condenser pipe, and a condensation outer wall that are arranged in nested manner; a distillation chamber is formed between the heating pipe and the tank body, a condensation chamber is formed between the heating pipe and the condensation outer wall, and a hollow portion of the condensation inner wall forms a reaction chamber; the heating pipe and the condensation inner wall are drove by the motor to rotate; the heating pipe is provided with a vapor outlet an evaporation residue channel is formed at an end of the heating pipe away from the feed inlet.

Fibroblast activation protein (FAP) targeting compounds; methods for imaging cancer and fibrosis; and methods for treating fibrosis, inflammatory diseases / disorders and cancer.

This invention discloses a polysaccharide esterified carbon dioxide / crude oil blending agent, its preparation method, and its application, relating to the technical field of carbon dioxide enhanced oil recovery (CEAR). The blending agent is an oligopolysaccharide esterified derivative or a mixture containing oligopolysaccharide esterified derivatives. The oligopolysaccharide molecule has one C12-18 long-chain ester group at one site and C2-C4 short-chain ester groups at the remaining sites. The blending agent is used to reduce the minimum miscibility pressure between crude oil and carbon dioxide. The preparation method involves using long-chain alkyl oligopolysaccharide esters and short-chain anhydrides as raw materials. Chain oligopolysaccharides are added to the reactor at a feed ratio of 1:3a+1, and cyclic oligopolysaccharides are added at a feed ratio of 1:3a-1. Copper perchlorate is used as a catalyst, and the reaction is carried out at 90-140℃ for 12-36 hours. After the reaction, the resulting product does not require purification to obtain the blending agent. The raw materials of this invention are readily available, the synthetic route is short, and the product can be used directly after the reaction without purification.

The present invention provides a method for obtaining a dealkoxyphenylated product in high yield from a substrate such as a sugar bonded via an oxygen atom to an alkoxyphenyl group. By reacting a lambda 3 -iodide with a substrate bonded via an oxygen atom to a phenyl group substituted with a C1-C5 alkoxy group in the para or ortho position in a fluorine-containing alcohol and water, a dealkoxyphenylated product can be obtained in high yield under mild conditions.

This invention belongs to the field of biomedical technology, specifically relating to a dihydroporphyrin e6 conjugate and its applications. Using dihydroporphyrin e6 and N-p-toluenesulfonyl ethylenediamine as raw materials, an N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6 conjugate is prepared by condensation; then, the N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6 conjugate is condensed with excess galactosamine hydrochloride to prepare an N-p-toluenesulfonyl ethylenediamine-dihydroporphyrin e6-digalactosamine conjugate. This conjugate exhibits improved water solubility and can target liver cancer cells and the intracellular endoplasmic reticulum, showing potential application value in the field of photodynamic immunotherapy for liver cancer.

The application discloses a chlorination process of sucrose-6-acetate, which comprises the following steps: preparing a Vilsmeier reagent by using DMF and chlorosulfuric acid under low-temperature conditions, releasing sulfur dioxide by heating, mixing the Vilsmeier reagent with 1,1,2-trichloroethane as a reaction raw material liquid, mixing the reaction raw material liquid with a pre-cooled DMF solution of sucrose-6-acetate through a pipeline mixer, performing high-temperature chlorination reaction and removing hydrogen chloride by using a three-stage continuous reactor, and sequentially neutralizing the chlorination liquid by using ammonia water and sulfuric acid, so that the target product can be obtained by controlling pH=7-7.5. The process flow is simple, the continuous process of low-temperature mixing, high-temperature reaction and neutralization reaction is realized, the complicated process of separating hydrogen chloride and sulfur dioxide can be omitted, the product yield is improved, the number of production equipment is reduced, the production cost is lowered, and the process has good industrial application value.