1714 results about "Carbohydrate" patented technology

The invention relates to the field of enzyme engineering, in particular to modified xylanase, a DNA (deoxyribonucleic acid) molecule, a recombinant plasmid and a transfected cell. The modified xylanase protected by the application is formed by replacing a deletion segment of a receptor enzyme with a substitution segment, the receptor enzyme is wild type xylanase, the sequence of the receptor enzyme is SEQ ID NO: 1, the deletion segment comprises a carbohydrate binding module of the receptor enzyme, the substitution segment is a carbohydrate binding module of a donor enzyme, the donor enzyme is a carbohydrate active enzyme, and the donor enzyme is a carbohydrate active enzyme. The sequence of the donor enzyme is one of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The carbohydrate binding module of the carbohydrate active enzyme with relatively high activity is replaced into the receptor enzyme, so that the binding capacity of the receptor enzyme and the carbohydrate is improved, and the activity of the receptor enzyme is improved.

Compositions and methods for treating diseases associated with aberrant glycosylation of cell surface molecules and expression of tumor-associated carbohydrate antigens (TACAs) are provided. Also provided are fusion proteins having specificity for tumor-associated carbohydrate antigen (TACA) comprising a half-life extender molecule, vectors encoding the TACA-fusion proteins, and recombinant cells comprising the TACA-specific fusion proteins.

The invention discloses a method for regulating the length-diameter ratio of calcium sulfate hemihydrate whiskers prepared from ardealite, and belongs to the field of ardealite resource utilization. According to the invention, carbohydrates, alcohols and other substances containing hydroxyl groups are used as the crystal modifier, and the calcium sulfate hemihydrate whisker is prepared by regulating and controlling phosphogypsum through a normal-pressure hydrothermal method, so that the length-diameter ratio of the whisker is regulated and controlled within a certain range. The method solves the problems that the length-diameter ratio of the whisker in the calcium sulfate hemihydrate whisker product prepared from ardealite is not uniform and cannot be regulated and controlled, the preparation process is tedious, the corrosivity is strong due to excessive salt solution in the reaction system, and the waste liquid has great harm to the environment, and has a good application prospect.

The present disclosure provides a recombinant yeast host cell for converting a carbohydrate to ethanol and acetone. The recombinant yeast host cell comprises an engineered metabolic pathway to convert acetyl-CoA to acetone, a downregulated native alcohol dehydrogenase (ADH) gene, and a heterologous alcohol dehydrogenase.

The invention provides a food composition and application thereof in promoting development of intestinal tract glycocalyx layers. The food composition comprises protein, fat, carbohydrate and dietary fiber, and further comprises lactose-N-neotetraose and 3 '-sialic acid lactose, and the ratio of the lactose-N-neotetraose to the 3'-sialic acid lactose is (1: 1)-(3: 1) by weight. In the food composition disclosed by the invention, the lactose-N-neotetraose and the 3 '-sialic acid lactose can be used for up-regulating the transcriptional level of genes, such as has1, has2 and has3, related to the development of the intestinal glycocalyx and the transcriptional level of ext2, so that the development of the intestinal glycocalyx is synergistically promoted.

The invention relates to the technical field of formula foods for special medical purposes, and discloses a low-GI formula food suitable for diabetics and a preparation method thereof, the low-GI formula food comprises the following raw materials by weight: 4-6% of protein, 2-4% of fat, 10-15% of carbohydrate, 2.5-4.5% of dietary fiber, 0.3-0.5% of mineral, 0.3-0.5% of vitamin, 3-5% of food additive, and the balance purified water. The full-nutrition formula food for the diabetes mellitus with special medical application meets the metabolic characteristics and nutritional requirements of people suffering from the diabetes mellitus in China, and provides a more scientific nutrition intervention means for people suffering from the diabetes mellitus in China.

The present invention belongs to the technical field of microorganisms, and provides a Bacillus subtilis FNFH_BS08 and use thereof. On the one hand, the present invention provides a Bacillus subtilis FNFH_B808, on the other hand, the present invention provides use of the Bacillus subtilis FNFH_BS08. The Bacillus subtilis FNFH_BS08 of the present invention can efficiently degrade main antigen proteins and non-starch polysaccharides in soybean meal by secreting highly active carbohydrate enzymes and proteases, thus improving the overall nutritional parameters of the soybean meal, improving the nutritional value of the soybean meal, and reaching a leading level in the field of fermentation of soybean meal by microorganisms.

The present invention relates to a novel composition for use as a whipping agent and for the production of whipped food products. The novel composition is characterized by the use of polyols as the carbohydrate source. The present invention further relates to a method of preparing the novel whipping agent.

A method for the manufacture of an oat peptide isolate, the oat peptide isolate comprising at least 80% w / w peptides, and less than 0.1% w / w total soluble fibre. The method comprises combining an oat protein concentrate having a protein concentration of at least 40% w / w with an enzyme capable of facilitating carbohydrate hydrolysis, removing the sugar fraction, and combining with an enzyme capable of peptide formation.

The invention discloses a method for detecting organic carbon components in soil based on gas chromatography-mass spectrometry, and particularly relates to the technical field of soil detection. The method comprises the following steps: pretreating fresh soil to obtain a fresh soil sample and an air-dried soil sample, respectively extracting organic carbon components to obtain a DOC extracting solution and an LOC extracting solution, and sequentially and respectively carrying out purification treatment, concentration treatment and derivatization on the DOC extracting solution and the LOC extracting solution to obtain a DOC to-be-detected solution and an LOC to-be-detected solution; respectively detecting the DOC to-be-detected liquid and the LOC to-be-detected liquid in a GC-MS instrument to obtain a total ion flow chromatogram, and carrying out qualitative and quantitative analysis to obtain organic carbon component data. Through step-by-step extraction, selective derivatization and GC-MS accurate analysis, molecular level separation, identification and quantification of water-soluble organic carbon DOC and oxidizable organic carbon LOC in soil are achieved, the method is particularly suitable for evaluating contribution of carbohydrate, amino acid and organic acid to the carbon turnover rate, and the method is good in selectivity and high in sensitivity.

The invention belongs to the technical field of food, and particularly relates to a high-satiety liquid protein composition as well as a preparation method and application thereof. The high-satiety liquid protein composition is characterized by being prepared from the following raw materials in parts by weight: 800 to 1000 parts of protein, 110 to 160 parts of vegetable oil, 700 to 800 parts of carbohydrate, 120 to 150 parts of dietary fiber, 5 to 15 parts of vitamin, 1 to 8 parts of mineral substance, 35 to 40 parts of emulsifier, 12 to 15 parts of stabilizer, 17 to 20 parts of pH regulator and 5 to 10 parts of essence. Under the comprehensive action of all the components, the liquid protein composition can keep the milk state stable, fat components are not subjected to lactation separation, protein components are not subjected to sedimentation and layering, the macroscopic state is stable, and nutrients in the product are uniformly distributed.

The invention discloses a feed for improving earthworm breeding yield and a preparation method thereof, and belongs to the technical field of earthworm breeding, the feed comprises the following components: 30-50 parts of fermented livestock and poultry manure, 20-30 parts of crop straw, 10-20 parts of soybean curb residue, 10-15 parts of corn flour, 5-10 parts of wheat bran, 3-5 parts of bone meal, 2-4 parts of shell powder, 0.5-1 part of a microbial agent, 1-2 parts of brown sugar, and a proper amount of water. The fermented livestock and poultry manure is rich in various mineral elements and organic nutrients such as nitrogen, phosphorus and potassium, and is an important source of nutrients required by earthworm growth; the crop straws are rich in cellulose and can promote intestinal peristalsis of the earthworms and help digestion; the soybean curb residues, the corn flour and the wheat bran are rich in nutritional ingredients such as protein and carbohydrate, and sufficient energy is provided for the earthworms; the bone meal and the shell powder contain a large amount of mineral substances such as calcium and phosphorus, so that the development of bones and bodies of the earthworms is facilitated; the microbial agent can regulate microbial flora in the feed and promote the absorption and utilization of the earthworms to nutrient substances.

The invention provides a method for efficiently purifying lignin by using a composite alkali system, and relates to the technical field of lignin extraction and purification, wood fiber biomass is used as a raw material, KOH / thiourea composite alkali liquor with a specific mass ratio is adopted, and directional dissociation reaction is carried out under the conditions that the solid-to-liquid ratio is 1: (5-15), the temperature is 60-150 DEG C and inert gas protection is adopted; and after the reaction is ended, through a gradient acidification process (controlling the pH to be 10-12 and the pH to be 3-5 in stages) and by combining centrifugal filtration and water washing, ash content and carbohydrate residues are remarkably reduced, and high-quality lignin with the purity being greater than or equal to 91.16% is obtained. The method solves the problems of low purity and high impurity residue in the traditional process, and provides core raw material guarantee for lignin-based capacitor carbon.

The invention discloses a food nutritional ingredient detection method, and relates to the technical field of food nutritional ingredient detection.The food nutritional ingredient detection method comprises the steps that time influence parameters of the degradation rate K, the biological value and the digestion rate under the food storage time T are obtained through a data collection module, and carbohydrates in the initial state of food are detected and analyzed through an analysis and detection module; the method comprises the following steps: calculating and evaluating the nutritional value of food in storage time T by using a calculation and evaluation module based on nutritional ingredient content parameters of protein and fat, time influence parameters and nutritional ingredient content parameters, and adjusting a food formula and storage conditions based on the nutritional value so as to improve the nutritional value of the food. According to the method, the time factor and the correlation coefficient are introduced, dynamic evaluation of the nutritional ingredient change of the food in the storage process is achieved, the evaluation accuracy is improved, a comprehensive evaluation system is established, the experimental process is simplified, and the model complexity is optimized.

The invention relates to the technical field of biology, and particularly discloses a method for removing lactulose-based isomers in an oligosaccharide separation and purification process. The method comprises the following steps: taking oligosaccharide fermentation liquor for producing oligosaccharide through fermentation by using lactose as a substrate, sequentially carrying out thallus removal, decoloration, electrodialysis desalination, ion exchange resin desalination, concentration, sequential simulated moving bed chromatographic purification, ultrafiltration, re-concentration and spray drying, and detecting the oligosaccharide and the lactulose-based isomer in the oligosaccharide. According to the method disclosed by the invention, the content of lactulose-based isomers in the separation and purification process is greatly reduced, so that the product purity is effectively improved; the method can be applied to the synthesis process of other milk oligosaccharides such as fucose lactose (FL), lactose-N-tetraose (LNT), lactose-N-neotetraose (LNnT), lactose-N-fucopentasaccharide (LNPFI) and the like or other carbohydrates containing a lactosyl group, has a very high application value, and accords with laws and regulations.

The invention describes a food or flour composition derived from fungal fermentation of plant material, the flour composition having a total metabolizable carbohydrate level of between 0.1-50% by weight. Also described are processes for the manufacture of the food or flour compositions using fungal fermentation having a total metabolizable carbohydrate level of between 0.1-50% by weight.

The invention relates to a particulate composition comprising, or substantially consisting of, urea, an inhibitor, preferably a urease inhibitor and / or a nitrification inhibitor, and an additive, wherein the additive comprises components (i), (ii), and optionally (iii): (i) one or more polyhydric alcohols; preferably one or more carbohydrates, preferably selected from monosaccharides, disaccharides, and oligosaccharides; (ii) a first polymer, preferably an amine-containing polymer; more preferably a polyvinylamine or a polyalkyleneimine; and (ii) optionally, a second polymer, preferably a polar polymer; more preferably a water-soluble polar polymer. The invention further relates to a process for producing the particulate composition, an additive for a particulate composition, and the use of the particulate composition as a fertilizer.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The present disclosure relates to low density, high protein, grain-based ready-to-eat food pieces, compositions including such food pieces, and methods of making such food pieces. The pieces include a grain flour in an amount of about 28% to about 55% by dry weight, about 28% to about 40% by dry weight protein, where at least a portion of the protein ingredients include a protein with a PDCAAS greater of at least 60%, such as pea protein, fava bean protein, or soybean protein, and about 10% to about 35% by dry weight low DE carbohydrate.

Disclosed herein are apparatuses and methods that can provide a delayed bolus calculator for use in closed loop diabetes therapy that can account for additional factors not taken into account in regular meal bolus calculations when a user wants to deliver a meal bolus a period of time after a meal was consumed. A delayed bolus calculator can enable the user to enter a period of time since the meal was consumed in addition to the number of carbohydrates consumed in a meal. This enables the system to account for the amount of increased insulin from the closed loop algorithm in response to the meal and / or the time since the meal to ensure that the risk of hypoglycemia is mitigated while still reducing hyperglycemia.

The present invention relates to the technical field of feeds and particularly relates to a piglet weaning transition feed containing lactic acid bacteria and saccharomycetes compound fermented soybean meal and a preparation method thereof, and the piglet weaning transition feed comprises the following components in parts by weight: 15-20 parts of compound fermented soybean meal, 45-50 parts of corn, 10-15 parts of wheat, 5-7 parts of fish meal, 3-5 parts of whey powder, 3-4 parts of vegetable oil, 1-2 parts of an amino acid supplement and 2-3 parts of a mineral premix, 1-2 parts of a vitamin premix and 1.5-2 parts of a calcium-phosphorus supplement; wherein the compound fermented soybean meal is prepared by performing compound fermentation on soybean meal through lactic acid bacteria and saccharomycetes, and a complementary and symbiotic microbial ecological system is formed by carefully selecting and optimizing the combination proportion of three lactic acid bacteria and two saccharomycetes, so that the fermentation efficiency and stability are improved, and the functionality of a fermentation product is enhanced. Different strains complement each other in the aspects of carbohydrate metabolism, pH tolerance and functional characteristics, and nutrition competition is reduced.

The invention discloses a method for producing D-pantoic acid by immobilizing D-pantoic acid lactone hydrolase fusion protein, and belongs to the technical field of biology. The insoluble seed pumpkin acidic polysaccharide PPFS3 is extracted from an agricultural waste, namely seed pumpkin pulp, meanwhile, a fusion protein constructed by connecting a carbohydrate binding module and D-pantoic acid lactone hydrolase is provided, and the fusion protein and the seed pumpkin acidic polysaccharide PPFS3 have specific binding capacity; a simple, convenient and low-cost immobilized enzyme and recombinant protein purification method can be established. The formed immobilized fusion protein can be used for efficient and continuous production of D-pantoic acid.

The invention discloses a method for converting livestock and poultry manure into an organic fertilizer through microbial fermentation, and belongs to the technical field of livestock and poultry manure conversion. The method comprises the steps that firstly, the livestock and poultry manure is subjected to physical sorting, lime or monopotassium phosphate is added according to the pH value detection result, and the pH value is adjusted to 6.5-7.5; after determining nutrient components of the feces, adding soluble salt trace elements such as zinc sulfate and mixed carbohydrates of cane sugar and glucose; traditional strains of bacillus, lactic acid bacteria, saccharomycetes and actinomycetes are mixed with novel strains separated from the periphery of a livestock and poultry farm, optimized culture is conducted through a two-step culture method, and plant extracts such as lily extracts or livestock and poultry intestinal metabolites such as short-chain fatty acid are added in the culture process. The fermentation process adopts a rotatable fermentation tank body, and comprises three stages of fermentation of rapid heating, high-temperature stabilization and cooling decomposition. And finally, low-temperature vacuum drying, crushing, granulation and addition of a coating material are performed to prepare the organic fertilizer, so that efficient resource utilization of the livestock and poultry manure is realized.

The invention relates to a fluorescent array sensor, in particular to a difunctional fluorescent array sensor containing a modified high-molecular polymer, a preparation method of the difunctional fluorescent array sensor and application of the difunctional fluorescent array sensor in bacterial detection and fermentation carbohydrate detection. The array sensor comprises at least two different fluorescent sensing units, each fluorescent sensing unit is a PN-coumarin dye compound formed by covalent interaction of PN and coumarin dye, PN is a nitrophenylboronic acid modified high-molecular polymer PEI, the coumarin dye is 6, 7-diphenyl-1, 3, 4-triazole-3-carboxylic acid, and the coumarin dye is a coumarin dye compound. The compound is selected from the group consisting of 2, 7-dihydroxy-2H-benzopyran-2-one ES or 4-methylesculetin MS. The compound fluorescent array sensor disclosed by the invention can simultaneously realize distinguishing detection of thirteen types of bacteria related to IBS and fourteen FODMAPs, and has the advantages of high sensitivity, short detection time, low cost, good repeatability, high accuracy, no need of professional technicians and the like.

Various processes for the pyrolysis of carbohydrates to prepare products such as glycolaldehyde are described. Also, various catalysts and processes for preparing catalysts useful for carbohydrate pyrolysis are described.

Non-aqueous membrane emulsion methods for producing polymeric and polymer-coated microparticles are provided. Some embodiments provide methods for producing a sustained release or controlled release microparticle by combining micronized protein powder and a polymer into a hydrocarbon solvent to form a non-aqueous first solution, agitating the first non-aqueous solution to form a suspension, feeding the suspension into a dispersion pump, wherein the suspension is infused through a porous membrane into a continuous phase comprising a fluorocarbon liquid and a fluorosurfactant to form a hydrocarbon-in-fluorocarbon emulsion. The hydrocarbon solvent, the fluorocarbon liquid, and the fluorosurfactant are removed, and the microparticles are collected.

The invention discloses a preparation method for directly preparing 2, 5-furandicarboxaldehyde from a carbohydrate, and the preparation method comprises the following steps: reacting a mixture I containing the carbohydrate, a catalyst, an organic phase solvent and a water phase solution in an oxygen-containing atmosphere to obtain the 2, 5-furandicarboxaldehyde, the catalyst comprises a component I and a component II; the component I is selected from at least one of aluminum chloride, indium chloride and chromium chloride; and the component II is an organic hydrophobic modified manganese oxide. According to the method, a 5-hydroxymethylfurfural intermediate product generated by reaction is directly oxidized without separation to prepare 2, 5-furandicarboxaldehyde, so that side reactions such as polymerization and the like are reduced; the carbohydrates are dissolved in the water phase and cannot be catalyzed and oxidized by the hydrophobic oxidation catalyst dispersed in the organic phase to generate byproducts, and the method has the advantages of low cost, mild reaction conditions, high product yield and the like.

The invention provides clarified defatted coconut milk, a preparation method thereof and application of the clarified defatted coconut milk in tea beverages. The preparation method of the degreased coconut milk comprises the following steps: S1, crushing and squeezing coconut meat to obtain coconut milk; s2, the coconut milk is filtered and centrifuged, a grease layer and an insoluble substance layer are removed, and degreased coconut milk is obtained; s3, sequentially adding pectinase and protease into the degreased coconut milk for enzymolysis, and filtering to obtain filtered pulp; and S4, adding a compound enzyme preparation into the filtered slurry, carrying out ultrasonic treatment, enzyme deactivation and filtration, centrifuging the filtrate, taking a clear juice layer, and filtering to obtain the clarified degreased coconut milk. According to the method, coconut pulp is taken from coconuts with proper fruit age and is juiced to prepare the coconut milk, and then proper filtration, clarification and centrifugal extraction conditions are combined, so that carbohydrates in the coconut milk can be better reserved, and substances such as protein and pectin in the coconut milk can be effectively removed or degraded, so that the degreased coconut milk becomes clear, and the quality of the coconut milk is improved. And turbidity or precipitation can be effectively avoided after the defatted coconut milk tea beverage is compounded.

The invention relates to the technical field of fermentation engineering, in particular to a method for analyzing carbohydrates in a 2 '-fucosyllactose product. The carbohydrate analysis method comprises the following steps: aiming at a 2 '-fucosyllactose product, separating carbohydrates through ion exchange chromatography, and the conditions are as follows: a mobile phase A comprises 4-6mM NaOH and 1-3mM NaAC; the mobile phase B is prepared from 80 to 120 mM of NaOH and 80 to 120 mM of NaAC; the elution procedure is a specific gradient elution procedure. According to the carbohydrate analysis method of the 2 '-fucosyllactose product, provided by the invention, miscellaneous sugar with relatively high similarity with the 2'-fucosyllactose in the 2 '-fucosyllactose product can be effectively separated, so that the analysis efficiency and accuracy of carbohydrates in the 2'-fucosyllactose product can be effectively improved; the method has important significance in the related production field of 2 '-fucosyllactose.

The invention discloses a lamb feeding method for increasing polyunsaturated fatty acid in meat. According to the invention, lactobacillus reuteri is supplemented into the basic feed for sheep, so that polyunsaturated fatty acids in mutton can be increased, the tenderness of the mutton can be improved, and the proportion and area ratio of I-type muscle fibers in muscles can be increased. Meanwhile, the technical scheme also can regulate the rumen microbial community structure of the sheep, promote the degradation of rumen carbohydrates and the generation of muscle aromatic compounds, and regulate the metabolism of sheep muscle proteins. The proper addition amount of lactobacillus reuteri in the basic feed for sheep is determined, the obtained optimized feed can improve polyunsaturated fatty acid and tenderness of mutton, the slaughter performance of sheep is enhanced, and the economic income of pasturing households and the economic benefit level of enterprises can be improved.