5959 results about "Enzyme" patented technology

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.

Multiple enzymes may be present in one or more active areas of an electrochemical analyte sensor for detecting one or more different analytes. In particular, an analyte sensor may comprise a sensor tail configured for insertion into a tissue and one or more working electrodes having a glucose-responsive active area and an ethanol-responsive active area to detect glucose and ethanol in vivo.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.

Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.

The invention discloses a nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of nano-enzymes. According to the preparation method, natural polyphenol, a cerium source and polyethylene glycol are mixed to prepare the novel polyphenol nano-enzyme, the preparation method is simple and easy to implement, the preparation cost is low, the prepared nano-enzyme has good biocompatibility, and a new way is provided for effective treatment of inflammatory diseases.

The invention discloses a multi-layer controllable degradation coating composition as well as a preparation method and application thereof, and belongs to the field of biodegradable high polymer materials and coating compositions. The coating composition comprises an outer protective layer and an inner functional layer, and the inner functional layer is arranged between a substrate and the outer protective layer and comprises a plurality of microcapsules. The key innovation lies in that the microcapsule encapsulates an enzyme capable of catalytically degrading the outer protective layer and is configured to be released after a predetermined delay time. The pH buffer system is integrated in the inner functional layer, and an optimal catalytic microenvironment is created for released enzyme, so that the outer protective layer is actively and quickly degraded, and programmable time-controlled removal is realized. The problem that the degradation rate of an existing biodegradable material is passive and uncontrollable is solved, and the coating has a remarkable application value in the field of seed coating or fertilizer controlled release by endowing the coating with a unique two-stage degradation characteristic.

The invention provides recombinant escherichia coli with high yield of ergothioneine and a method for preparing ergothioneine, the recombinant escherichia coli co-expresses ergothioneine anabolic enzymes Egt1 and Egt2, the methyltransferase structural domain of the Egt1 is derived from trichoderma reesei, mycobacterium smegmatis EgtD or rhizopus nigricans, and the methyltransferase structural domain of the Egt2 is derived from the trichoderma reesei, the mycobacterium smegmatis EgtD or the rhizopus nigricans. The histidine sulfoxide synthase structural domain is derived from trichoderma reesei, schizosaccharomyces pombe or rhizopus nigricans, and the Egt2 is derived from claviceps purpurea, neurospora crassa or aspergillus niger. After the recombinant escherichia coli obtained by screening is cultured for 68 hours in a 5L fermentation tank, the yield of ergothioneine can reach 5g / L or above; wherein after recombinant escherichia coli co-expressing Egt1 enzyme (the structural domain of methyltransferase is derived from mycobacterium smegmatis EgtD, and the structural domain of histidine sulfoxide synthase is derived from trichoderma reesei) and Egt2 enzyme (derived from neurospora crassa) are cultured in a 5L fermentation tank for 68 hours, the yield of ergothioneine can reach 8.7 g / L, and the production intensity reaches 127.9 mg / (L.h).

The invention discloses a method and a system for adjusting a tea leaf fixation process and a medium, and aims to realize cooperative regulation and control of enzyme activity and aroma substances in a fixation process. The method comprises the following steps: collecting multi-modal sensing information in a fixation process, and carrying out feature extraction on the multi-modal sensing information to obtain multi-dimensional features; dynamically constructing an LSTM prediction model based on the multi-dimensional features, and outputting an enzyme activity prediction value sequence; the enzyme activity predicted value sequence is input into a fuzzy PID controller for reinforcement learning optimization, a temperature-rotating speed adjusting instruction is generated, and adjusting parameters of the controller are dynamically updated through a reward function of the chlorophyll retention rate and the energy consumption ratio; according to the temperature-rotating speed adjusting instruction, adjusting the hot air temperature of each temperature area of the enzyme deactivation chamber, the rotating speed of the enzyme deactivation cylinder and the axial segmentation wind speed; and collecting fixation end point judgment information, and terminating fixation when a preset condition is met. According to the invention, the problems of unstable quality and overhigh energy consumption caused by parameter solidification in the traditional enzyme deactivation process are solved, and dynamic optimization and accurate control of the enzyme deactivation process are realized.

The invention discloses a formate dehydrogenase mutant and application thereof in catalytic synthesis of D-mannitol, and belongs to the technical field of bioengineering. According to the formate dehydrogenase mutant provided by the invention, the 18th-site glutamic acid of wild-type formate dehydrogenase with an amino acid sequence shown as SEQ ID NO.2 is mutated into proline, the 57th-site asparagine is mutated into glutamic acid, the 70th-site histidine is mutated into tryptophan, the 235th-site serine is mutated into threonine, and the 316th-site valine is mutated into threonine, so that the formate dehydrogenase mutant is high in enzyme activity and catalytic activity; the coenzyme circulation efficiency during the production of D-mannitol can be obviously improved, so that the yield of D-mannitol can be effectively improved. When the genetically engineered bacterium co-expressed by the formate dehydrogenase mutant and the mannitol dehydrogenase mutant is used for catalyzing 150 g / L fructose to produce D-mannitol, the yield of D-mannitol within 15 h can reach 140 g / L or above.

The invention discloses preparation and application of bionic cerium-based polyphenol nano-enzyme for treating rheumatoid arthritis, and belongs to the field of medical preparations. The method comprises the following steps: respectively complexing cerium ions and tannic acid or epicatechin gallate to prepare cerium-based polyphenol nano-enzyme, and then preparing the cerium-based polyphenol nano-enzyme wrapped by a macrophage membrane. And loading the cerium-based polyphenol nano-enzyme wrapped by the macrophage membrane into the hydrogel to prepare the hyaluronic acid-based hydrogel. The cerium-based polyphenol nano-enzyme and the hyaluronic acid-based hydrogel are characterized, it is verified that the cerium-based polyphenol nano-enzyme and the hyaluronic acid-based hydrogel are successfully synthesized, the particle size of CeTA is about 22 nm, the particle size of M-CeTA is increased to 273 nm, the particle size of CeECG is about 95 nm, and the particle size of M-CeECG is increased to 292 nm. In-vitro experiments prove that the M-CeTA and the M-CeECG do not show obvious cytotoxicity when being less than 80 mu g / mL, and have an obvious inhibition effect on expression of cell inflammatory factors on inflammatory cells.

Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.

The invention discloses a method for efficiently screening saline-alkaline tolerant rice. According to the method, controllable indoor saline-alkali environment simulation conditions are constructed, various salts are mixed in proportion to prepare solutions with different salinity gradients, the pH value is adjusted, and temperature, humidity and illumination parameters are accurately controlled; the method comprises the following steps: disinfecting rice seeds, carrying out a germination test, transplanting rice seedlings, and regularly measuring growth indexes; in the treatment process, physiological indexes such as malondialdehyde content, antioxidant enzyme activity, proline content and the like and the expression level of salt and alkali tolerance related genes are detected, key indexes are screened by applying statistics and multivariate analysis methods, and an evaluation system is established for comprehensive score screening. The method can quickly perform saline-alkali stress treatment on a large number of rice seeds or seedlings, is not limited by natural environment, shortens the screening period, can accurately simulate different saline-alkali soil environments, scientifically evaluates the saline-alkali tolerance of rice from multiple dimensions, and provides an efficient and reliable technical means for breeding saline-alkali tolerant rice varieties.

The invention provides an expansible ring enzyme mutant and an application of the expansible ring enzyme mutant in synthesis of G-7-ADCA. A series of mutants with penicillin G ring expansion activity are obtained through ancestor enzyme sequence reconstruction, directed evolution and the like, and the mutants can directly expand the ring of the substrate penicillin G to generate G-7-ADCA. Therefore, the expansive ring enzyme mutants have important application value in industry.

The invention relates to a preparation method of Pt monatomic nano-enzyme and application of Pt monatomic nano-enzyme in cardiac troponin I. The preparation method comprises the following steps: 1) ultrasonically dispersing cobalt salt, selenium powder, chloroplatinic acid, urea and sodium chloride in an alcohol solvent, heating and stirring until the solvent is completely volatilized to obtain a solid mixture, and grinding the solid mixture into powder; and 2) placing the powder in a heating device, carrying out Joule heat treatment in an air atmosphere, washing a reactant, and carrying out vacuum drying to obtain the Pt monatomic nano-enzyme. Se doping is introduced in the one-step chemical reaction process to construct oxygen vacancies, rapid heating and structural shaping are achieved in combination with Joule heat treatment, the Se-doped Co3O4-loaded Pt monatomic catalyst is obtained, the oxygen vacancies and Se doping sites are synergistically used as anchoring sites, Pt monatomic atoms are effectively fixed, and the loading capacity and dispersity of the Pt monatomic atoms are improved. The obtained material shows excellent POD-like enzyme activity, can be used for constructing a high-sensitivity colorimetric immunoassay system, and realizes rapid and accurate detection of cTnI.

The invention discloses injectable hydrogel with redox activity and electroactivity as well as a preparation method and application of the injectable hydrogel, and belongs to the technical field of medical materials. The preparation method comprises the following steps: reacting methacrylic anhydride and polysaccharide molecules in a solvent to prepare a double-bond functional polymer; the preparation method comprises the following steps: firstly, pre-polymerizing dopamine in a solvent, and then adding MOF (Metal Organic Framework) to carry out polymerization reaction, so as to prepare dopamine modified MOF; reacting the dopamine modified MOF, EDOT and ferric salt in a solvent, and collecting the precipitate to prepare a nano filler; the preparation method comprises the following steps: jointly dispersing the double-bond functional polymer, active polypeptide, a photoinitiator and nano filler into a solvent, and carrying out photocuring cross-linking. The preparation condition is mild, gel forming is rapid, the method is simple, the prepared hydrogel has injectable performance and conductive performance, catalytic activity of natural enzyme can be simulated, active oxygen removal can be achieved in the early stage of myocardial ischemia, electrical conduction of infarction parts can be enhanced, and effective recovery of cardiac functions can be achieved.

The invention discloses a hydroxysteroid dehydrogenase mutant with improved thermal stability, a screening method and application, key hotspot amino acid residues related to the thermal stability are rapidly identified based on computer-aided design technologies such as hydroxysteroid dehydrogenase structure prediction, PROSS and the like, so that a limited site-directed mutant library is constructed; and further screening to obtain a dominant mutant. The semi-inactivation temperature (T5105) of the obtained mutant is increased by 1.0-7.7 DEG C compared with that before mutation, wherein the semi-inactivation temperatures (T5105) of the mutants A126I and A132H are increased by 7.7 DEG C and 3.7 DEG C respectively. And the relative enzyme activity of the mutant reaches 99.86-136.73% of the relative enzyme activity before mutation. The invention provides a new method for rapidly improving the thermal stability of the enzyme, and lays a foundation for the industrial application of the hydroxysteroid dehydrogenase.

The invention belongs to the technical field of enzyme engineering, and discloses an MHETase-EstD9 mutant and an application of the MHETase-EstD9 mutant. The invention provides an MHETase-EstD9 mutant, the amino acid sequence of the MHETase-EstD9 mutant is shown as SEQ ID No.2, and on the basis of the mutant, the amino acid of the MHETase-EstD9 mutant is further mutated to design five MHETase-EstD9 mutants. At 70 DEG C, compared with wild type MHETase-EstD9, the BHET degradation activity of the MHETase-EstD9 mutant provided by the invention is improved by 15% or more, and the highest BHET degradation activity can be improved by 24 times; meanwhile, the thermal stability of the MHETase-EstD9 mutant is also improved to a certain extent, and the Tm value is maximally improved by 18.0 DEG C. The method can be applied to the fields of degradation of PET, MHET or BHET, preparation of degradation agents of PET and degradation products thereof, TPA recovery and the like, and has important environmental benefits, economic benefits and social benefits.

The invention discloses a method for extracting astaxanthin by using a biological enzyme, which comprises the following steps of: decomposing cell walls of haematococcus pluvialis at a certain temperature by using the biological enzyme, releasing astaxanthin from the haematococcus pluvialis, dissolving out the astaxanthin by using a certain solvent, and separating and purifying to obtain powdery astaxanthin. According to the method disclosed by the invention, wall breaking is carried out by combining ultrasound and biological enzyme, and a mixed solvent of ethanol and Tween 80 is adopted, so that the extraction rate of astaxanthin reaches 98.1%, the wall breaking time is shortened, and the efficiency is improved.

The invention discloses a composite denitrification accelerant and a preparation method thereof, and belongs to the technical field of sewage treatment. The carbon source composite material is prepared from the following raw materials in parts by weight: 30-50% of a carbon source, 2-5% of vitamins, 2-5% of amino acids, 1-3% of a biological stimulant, 1-2% of signal molecules, 1-5% of metal elements, 3-5% of a pH regulator and 25-60% of water. Through metabolites such as vitamins, amino acids and biological stimulants, growth and reproduction of denitrifying bacteria are promoted, and functions of related enzymes in the denitrification process are improved. The signal molecule optimizes the metabolic activity of bacteria by regulating and controlling the expression of genes, related to the denitrification process, of the denitrifying bacteria. The metal ions can enhance the activity of the denitrifying enzyme, so that the efficiency of denitrification treatment is improved. According to the denitrification accelerant, the denitrification process is promoted through the synergistic effect of three aspects of growth and reproduction of denitrifying bacteria, expression of denitrification genes and improvement of activity of denitrification-related enzymes.

The invention discloses a Burkholderia gladioli (Burkholderia gladioli) CT-5 strain and an application of the Burkholderia gladioli CT-5 strain, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242732, and the preservation date is December 05, 2024. The Burkholderia gladioli (Burkholderia gladioli) CT-5 provided by the invention has a remarkable prevention and treatment effect (Plt, Plt) on idesia root rot caused by Fusarium solani (Fusarium solani), and the Burkholderia gladioli (Burkholderia gladioli) CT-5 has a remarkable prevention and treatment effect on idesia root rot caused by Fusarium solani (Busarium solani). The compound has the advantages that the compound can be used for inhibiting pathogenic bacteria of various plants, and the compound can be used for inhibiting pathogenic bacteria of various plants, such as Schizophyllum commune, Diaporthe erens, Colletotrichum acutatum and the like, and has a remarkable inhibiting effect on other various plant pathogenic bacteria such as the Schizophyllum commune, the Diaporthe erens, the Colletotrichum acutatum and the like. In a pot experiment, the biomass, soil nutrients, SOD, POD and CAT enzyme activities of idesia polycarpa seedlings in a CT-5 treatment group are remarkably improved compared with those of a pathogenic bacteria control group (FP) (Plt; 0.05), which indicates that the strain can promote plant growth and activate the activity of defensive enzyme in the body, so that effective control of idesia root rot is realized, and powerful theoretical basis and technical support are provided for sustainable development of idesia.

Methods for stimulating plant growth and / or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and / or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

The invention discloses a monoclonal antibody BY017 combined with African swine fever virus P30 protein and application of the monoclonal antibody BY017. The invention provides an IgG antibody which is composed of a light chain and a heavy chain. CDR1, CDR2 and CDR3 in the heavy chain variable region are sequentially shown as the 47 to 54 , the 72 to 79 and the 118 to 126 in SEQ ID NO: 1; cDR1, CDR2 and CDR3 in a light chain variable region are sequentially as shown in the 49 to 60 , the 78 to 80 and the 117 to 124 in SEQ ID NO: 3. The invention also protects the application of any one of the IgG antibodies in preparation of products. The product has the functions that the product is used for being combined with an African swine fever virus P30 protein fragment; the antibody is used for binding African swine fever virus P30 protein; the antibody is used for binding African swine fever virus. The IgG antibody provided by the invention can be used for multiple purposes of western blot, immunohistochemistry, flow cytometry, ELISA (Enzyme-Linked Immunosorbent Assay) and the like, and can be used as an important tool for ASFV detection and structural analysis of P30 protein of ASFV detection.

The invention belongs to the technical field of biology, and particularly relates to application of a PdbUNE12 gene in improving the salt tolerance of populus sutchuenensis and a method for improving the salt tolerance of populus sutchuenensis. The nucleotide sequence of the PdbUNE12 gene is as shown in SEQ ID NO. 1 (Sequence Identifier Number 1). The invention finds that the overexpressed PdbUNE12 gene can reduce the electrolyte permeability of a plant under salt stress, can reduce the malondialdehyde content of the plant under salt stress, and can improve the SOD enzyme activity of the plant under salt stress, so that the invention provides the application of the PdbUNE12 gene in improving the salt tolerance of populus sutchuenensis.

The invention discloses an intelligent gradient extraction process and method for nutritional ingredients in yak bone marrow, and belongs to the technical field of bioactive substance extraction. The extraction method comprises the following four steps: gradient pressure supercritical degreasing, double-enzyme synergistic gradient enzymolysis, acid concentration gradient demineralization and temperature gradient gelatin extraction, and through the process coupling design of gradient pressure degreasing, buffer enzymolysis, graded acidolysis and membrane separation of gelatin, the high yield of the product in the previous stage is ensured; active structures of follow-up components are reserved to the greatest extent. Meanwhile, a sensing-decision-execution closed-loop system is constructed, real-time data sources such as NIR moisture sensing, online OPA detection and XRD in-situ analysis are integrated, technological parameters are dynamically adjusted through a multi-objective optimization algorithm, and global optimization of the yield, the purity and the energy consumption is achieved.