25 results about "Fermentable sugar" patented technology

The invention relates to the technical field of wine brewing, and discloses a bamboo leaf powder added distilled liquor and a preparation method thereof, and the distilled liquor is prepared from 75-85 parts of grain powder and 15-25 parts of enzyme-deactivated bamboo leaf powder. The preparation method comprises the following steps: mixing the raw materials to obtain a dry mixture; adding a compound enzyme preparation containing cellulase and pectinase, preserving heat at 50-55 DEG C, performing enzymolysis and moistening, destroying a fiber structure and converting fermentable sugar; cooking the moistened material, and adding a saccharifying enzyme for saccharifying; primary fermentation is performed at 28-32 DEG C, and then secondary fermentation is performed at 22-25 DEG C; during distillation, foreshot with the expected wine yield of 1.5%-2.0% is intercepted and abandoned to remove methanol, fractions with the volume of 50% or above are collected to obtain raw wine, and the wine is obtained through ageing. The raw material utilization rate and the wine yield are improved through enzymolysis, the methanol content is controlled through temperature-variable fermentation and precise cut distillation, the raw and green taste of bamboo leaves is eliminated, and the finished wine has the unique composite style of bamboo fragrance and grain fragrance.

The present invention produces fermented kombucha beverages with reduced alcohol content by utilizing allulose or analogues thereof as the primary fermentable sugar. These rare sugars limit ethanol production during fermentation while preserving flavor, probiotic benefits, and acidity. The invention further encompasses the incorporation of Kratom (Mitragyna speciosa) extracts or alkaloids into the fermentation process or final product, enabling a low-alcohol kombucha with potential enhanced wellness properties. The resulting beverage maintains an alcohol content below 0.5% by volume, making it suitable for non-alcoholic labeling and broader consumer appeal. The invention is preferably a bottled beverage.

The invention is suitable for traditional Chinese medicine residue resource utilization and biological refinery technology, and provides a preparation method of a high-protein and high-fat feed. The method comprises the following steps: adding a certain amount of cheap nitrogen source into plant source wet traditional Chinese medicine residues rich in starch to prepare a fermentation culture medium, inoculating trichoderma reesei and lipomyces starkeyi for mixed culture, and performing combined action on cellulase produced by the trichoderma reesei and amylase produced by the lipomyces starkeyi on the traditional Chinese medicine residues, so as to obtain a fermentation product. And rapidly degrading into fermentable sugar which is efficiently used for microbial growth and grease and protein production, then carrying out solid-liquid separation, and drying the solid to obtain the high-protein and high-grease feed. Wet traditional Chinese medicine residues rich in starch are used as raw materials, cellulase and amylase production, enzyme hydrolysis and fermentation are highly integrated, the number of equipment is reduced, the production and processing flow is simplified, and the production cost of the high-protein feed is remarkably reduced. The fermented product serving as the feed is rich in protein and microbial oil, the fatty acid composition of the microbial oil is similar to that of vegetable oil, the nutritional ingredients of the feed are further improved, and rapid growth of poultry can be efficiently promoted. The method can promote the resource utilization of the cheap traditional Chinese medicine residues, provides a low-cost new method for improving the feed quality, and has remarkable economic benefits.

The invention discloses an Actinomyces sp. Strain, a separation and purification method and application of the Actinomyces sp. Strain in straw degradation, the Actinomyces sp. Strain is Actinomyces sp. And has been preserved in the China General Microbiological Culture Collection Center on July 3, 2025, and the preservation number of the Actinomyces sp. Strain is CGMCC No. 46460. As facultative anaerobic bacteria, the Actinomycete disclosed by the invention can be used for realizing efficient degradation under an anaerobic or aerobic condition in a single system, so that the process is remarkably simplified. In the practical application process, the strain can be used as a functional strain to be added into straw compost, organic matter decomposition is promoted, the composting period is shortened, and meanwhile the humus content is increased; fermentable sugar is generated by pretreating straws and is used for producing bioethanol or biogas; and the degraded straw residues can enhance the water-retaining property and microbial activity of the soil.

A method is disclosed for providing a c5 / c6 product from a lignocellulosic material, said method comprising the steps of: (i) pretreatment of the lignocellulosic material; (ii) solid / liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction; (iii) enzymatic hydrolysis of the fiber from said first solid fraction from step (b) using an enzyme composition capable of degrading lignocellulosic material, thereby providing a c5 / c6 fiber paste comprising c5 and / or c6 sugars; (iv) solid / liquid separation of the c5 / c6 fiber paste from step (c) into a second solid fraction and a second liquid fraction; and optionally (v) combining said first liquid fraction and said second liquid fraction for enzymatic hydrolysis of mixed sugar (msh), thereby providing a c5 / c6 msh product.

The gynura formosana fermentation equipment comprises a fermentation tank, a support is rotationally connected to the fermentation tank, a mounting table is fixedly mounted on one side of the support, a driving assembly is mounted on the mounting table, the output end of the driving assembly is connected with one end of the fermentation tank to drive the fermentation tank to rotate, and the fermentation tank comprises a tank body; a stirring assembly is mounted in the tank body. The gynura formosana fermentation equipment has the advantage of efficient mixing, and the problems that in the using process of existing gynura formosana fermentation equipment, gynura formosana raw materials and lactic acid bacteria strains are inconvenient to efficiently mix, lactic acid bacteria cannot make uniform contact with fermentable sugar on the surfaces of gynura formosana cut sections, strains in part of areas cannot grow in time, and the fermentation effect is poor are solved. And local concentration nonuniformity of metabolites can be caused.

This invention discloses a method for promoting the hydrolysis of lignocellulose into fermentable sugars, belonging to the field of biorefining technology. The method includes the following steps: (1) adding ammonium sulfate to the lignocellulose raw material, then mixing it with a glycerol-water mixture, and pretreating it at a certain temperature and time to destroy the dense structure of the lignocellulose; (2) adding ammonium sulfate and cellulase to the pretreated system, and carrying out a saccharification reaction under suitable conditions to convert it into fermentable sugars. This invention solves the problems of high energy consumption, high pollution, and low saccharification and enzymatic hydrolysis efficiency of traditional pretreatment by leveraging the solubilizing, crystallinity-reducing, and biocatalyst-protecting effects of glycerol and the synergistic effect of ammonium sulfate. Compared with traditional hot water pretreatment, the yield of fermentable sugars is significantly increased, and the pretreatment process does not require strong acids or bases; glycerol can be recycled and reused, significantly reducing production costs and possessing significant industrial application value.

The invention discloses a preparation method of corn vinegar through lactic acid bacteria synergistic fermentation in combination with ultrasonic-microwave accelerated aging treatment, the method adopts an ultrasonic-microwave accelerated aging treatment technology to prepare novel corn vinegar, corn is taken as a raw material, liquefaction and saccharification stages are optimized through a two-enzyme method, and efficient conversion from corn starch to fermentable sugar is realized; lactobacillus plantarum is used for synergistically performing alcoholic fermentation to generate a plurality of flavor substances such as lactic acid and ethyl lactate; and the aging process of the corn vinegar is accelerated by combining ultrasonic-microwave aging accelerating treatment. Wherein ultrasonic waves can initiate a local high-temperature and high-pressure effect and a medium cavitation effect, so that polar molecules are orderly arranged, the molecular activation energy is improved, reactions such as esterification, condensation and oxidation reduction are accelerated, the energy consumption is relatively low, and the green production requirement is met; the microwave treatment breaks the association state of molecular groups in the original system through a microwave generator, and further accelerates the alkyd esterification process. The method solves the problems of unstable components and inharmonious smell in newly brewed corn vinegar.

The present application relates to fruit wine brewing technical field, particularly to a kiwi fruit wine and its brewing method. The present application takes kiwi fruit dregs as raw material, mixes the dregs with water, then adds fermentable sugar and pectinase for first enzymolysis, after first low-temperature fermentation, adds pectinase again for second enzymolysis, adds fermentable sugar and wine yeast seed culture solution for second low-temperature fermentation, then adds honey for third low-temperature fermentation, finally ages and filters to obtain kiwi fruit wine. The present application realizes high-value utilization of kiwi fruit dregs through twice enzymolysis and three times gradient low-temperature fermentation, the product has moderate alcohol content, good active ingredient retention, excellent flavor, and is suitable for industrial production.

Previously, methods have been described for increasing the amount of fermentable sugars in a fermentation substrate by treating it with a combination of an enzyme having transglucosidase activity and an enzyme having glucoamylase activity to hydrolyze oligosaccharides and / or polysaccharides that are not hydrolyzed by glucoamylase alone during fermentation. This method is most effective using a fermentation substrate containing small amounts of maltose and maltotriose.

A process for producing a fermentation product and a concentrated protein co-product from a starch-containing material e.g. by (a) liquefying a starch-containing material at a temperature above the initial gelatinization temperature of the starch with an alpha-amylase to produce a liquefied mash; (b) subjecting the liquefied mash to a protein concentration process to produce a concentrated protein co-product, a soluble starch stream and a sugar stream; (c) saccharifying the soluble starch stream and the sugar stream with a glucoamylase to produce a fermentable sugar; (d) fermenting the sugar with a fermenting organism to produce the fermentation product, wherein at least one separation step is performed after the liquefaction in step (a) resulting in a liquid portion comprising soluble starch / oligosacchrides and an insoluble portion comprising solids from the liquefied mash, said insoluble portion undergoing said protein concentration process.

A process for producing beer, the process comprising; (i) providing a mash comprising malt and water, (ii) adding a calcium carbonate material in an amount of from about 30 ppmw to about 70 ppmw, based on the total weight of the mash; and (iii) mashing the mash at a temperature of about 50°C to about 70°C and at a pH from about 5 to about 6 to produce an intermediate product comprising a wort and solids, wherein the wort comprises water and fermentable sugars; wherein the calcium carbonate material is; added to the mash comprising malt and water directly; or added to water used to form the mash; and wherein the calcium carbonate material comprises in the range of about 0.3 wt.% to about 0.75 wt.% Mg2+, based on the total weight of the calcium carbonate material, as measured by inductively coupled plasma.

The application discloses a method for synchronously and efficiently metabolizing mixed sugar based on a Pichia pastoris mixed flora and application thereof. The Pichia pastoris mixed flora comprises at least one Pichia pastoris engineering strain for xylose metabolism and one Pichia pastoris engineering strain for oligosaccharide metabolism. By means of functional division of the strains, the interference of glucose signal on the xylose metabolic pathway is physically and genetically isolated, and the synchronous metabolism of xylose and cellulosic oligosaccharide is realized. The metabolic burden of each strain is reduced, the exclusive pathway of each strain can be optimized, the pathway competition and the imbalance of auxiliary factors are avoided, the overall fermentation rate and the sugar conversion rate are improved. The mixed flora system has a certain buffering capacity to environmental fluctuations and is more stable than a single strain. The strategy is particularly suitable for complex lignocellulose hydrolysate, and can effectively solve the problem of asynchronous utilization of multiple fermentable sugars in the lignocellulose hydrolysate and improve the total utilization rate of raw materials. In the process of simultaneous saccharification and fermentation of the mixed flora, the utilization rate of xylose and cellulosic oligosaccharide is improved, and the integrated biorefinery cycle is shortened.

The invention relates to a method for high-density culture of saccharomyces cerevisiae by using potato residue saccharified liquid. The method comprises the following steps: S0, providing potato residues; the method comprises the following steps: S1, adding pectinase to degrade pectin; s2, medium-temperature amylase and cellulase are added for synchronous liquefaction and cellulose pretreatment; s3, glucoamylase and hemicellulase are added for deep saccharification; s4, performing solid-liquid separation to obtain potato saccharified liquid; and S5, taking the potato saccharified liquid as a main carbon source, inoculating a saccharomyces cerevisiae seed solution, and carrying out continuous fed-batch fermentation. According to the method, a synergistic enzymolysis strategy is adopted, firstly, pectinase is utilized for pretreatment to destroy a cell wall structure, then medium-temperature amylase and cellulase are subjected to synergistic liquefaction, and finally, saccharifying enzyme and hemicellulase are combined for deep saccharification, so that starch, cellulose and other components which are tightly combined in the potato residues are fully released and converted into fermentable sugar.

The invention discloses a coupling in-situ enzyme hydrolysis method for low-organic-acid pretreatment of agricultural and forestry wastes under the assistance of a low-concentration auxiliary agent. The method comprises the following steps: by taking crushed agricultural and forestry waste as a raw material, adding an aqueous solution containing a low-concentration auxiliary agent and organic acid into the raw material, reacting at 140-185 DEG C for 0.5-4.0 hours for pretreatment, cooling to 45-55 DEG C after the reaction is finished, adding cellulase accounting for 5-25 mg / g of the raw material into the raw material, hydrolyzing at 50 DEG C for 72 hours, filtering, washing, and drying to obtain the cellulose. And after enzyme hydrolysis is finished, carrying out solid-liquid separation to obtain solid residues and enzyme hydrolysate, adjusting the pH value of the enzyme hydrolysate, and carrying out oil-producing fermentation. According to the method, organic acid pretreatment is assisted by the aid of the auxiliaries, so that pollution or fermentation inhibition caused by alkali, solvents or inorganic acid is avoided, meanwhile, the enzyme hydrolysis efficiency is improved by the aid, and fermentable sugar with higher concentration is obtained.

The application discloses a method for co-producing high-quality lignin and fermentable sugar by glyoxylic acid-assisted eutectic solvent pretreatment of wood fiber, and belongs to the technical field of clean separation and high-value utilization of wood fiber raw materials. In the application, a glyoxylic acid-assisted acidic eutectic solvent system is used to pretreat wood fiber raw materials, the pretreatment liquid is subjected to rotary evaporation, and proper deionized water is added to precipitate lignin, which is subjected to water washing and freeze drying to obtain recovered lignin. Compared with a system without the addition of glyoxylic acid, the application can significantly improve the dissociation effect of the acidic eutectic solvent on the wood fiber raw materials at a lower glyoxylic acid addition amount, greatly improves the lignin removal rate and the quality of the lignin, and the introduction of glyoxylic acid helps to retain the carbohydrate retention rate of the wood fiber raw materials, and more than 90% of the carbohydrates are converted into corresponding monosaccharides through subsequent enzymatic saccharification.

The application discloses a coupling method for producing fructo-oligosaccharide and feed yeast by using water extract of moringa oleifera branch, and belongs to the field of high-value utilization of agricultural and forestry by-products and biological processing technology. The method comprises the following steps: water extraction is performed on the moringa oleifera branch to obtain a water extract of the moringa oleifera branch containing fermentable sugar; then, a fructosyltransferase is used for enzymatic reaction to obtain an enzyme cut solution containing fructo-oligosaccharide; then, the enzyme cut solution is inoculated with saccharomyces cerevisiae for fermentation; after the fermentation is completed, the fermentation liquid is separated into solid and liquid, the supernatant is a fructo-oligosaccharide solution, and the part of the bacteria is centrifuged, washed and dried to obtain feed yeast. The method realizes efficient utilization of natural sugar components in the moringa oleifera branch and the synergistic preparation of fructo-oligosaccharide and feed yeast, and has the advantages of wide raw material sources, mild process, high added value and high resource utilization rate.

The invention discloses a compound bacteria fermented fruit and vegetable marinade and a preparation method thereof, and particularly relates to the technical field of fermentation engineering. The method comprises the following steps: firstly, adding a proper amount of water into raw materials rich in fermentable sugar, sulfur-containing flavored vegetables and prebiotic sources, and beating into paste to serve as fruit and vegetable-based bottom mud; inoculating lactic acid bacteria and saccharomycetes which are subjected to induction culture into the fruit and vegetable-based bottom mud, and performing aerobic fermentation and anaerobic fermentation to obtain a fermentation product; finally, adding common food additives into a fermentation product, performing low-temperature refrigeration and after-ripening, performing heat treatment at 85-95 DEG C for 15-30 seconds, killing viable bacteria, and stopping fermentation; and cooling to room temperature to obtain the compound bacteria fermented fruit and vegetable marinade. According to the method disclosed by the invention, lactic acid bacteria (lactobacillus plantarum and lactobacillus fermentum) and saccharomycetes (Wickerhamomyces anomalus) are subjected to induced culture respectively, so that the lactic acid bacteria and the saccharomycetes are subjected to adaptive pre-domestication based on interaction signals, and the ecological compatibility and metabolism synergistic efficiency of the lactic acid bacteria and the saccharomycetes in a co-fermentation system are remarkably improved.

The invention relates to a method for dispensing an alcohol-free beer from a beer container comprising 4-10,000 L of the alcohol-free beer until the container is emptied during a dispensing period wherein the dispensing period exceeds 12 hours and wherein the beer container is maintained at ambient temperature during the dispensing period, said alcohol-free raw beer comprising: 5-150 g / L fermentable sugar, the components are selected from glucose, maltose, fructose, cane sugar and combinations thereof; 0.1 to 50 mg / L of one or more glycolipids selected from the group consisting of:-a glycofatty acid according to formula (I)-a lactone obtained by molecular lactonization of a glycofatty acid according to formula (I)-a physiologically acceptable salt of a glycofatty acid according to formula (I); -an ester of a sugar fatty acid according to formula (I); and 0.1 to 50 mg / L of one or more reduced iso-alpha acids selected from the group consisting of a dihydroiso-alpha acid, a tetrahydroiso-alpha acid and a hexahydroiso-alpha acid. By incorporating this combination of glycolipids and reduced iso-alpha acids into the beer, undesirable yeast fermentation in non-alcoholic raw beer can be prevented very effectively without any adverse effect on the quality of the raw beer.

The invention discloses a method for preparing fermentable sugar from forest biomass, which comprises the following steps: 1) cleaning, drying and crushing forest biomass to obtain forest biomass powder; 2) dispersing the forest biomass powder in an aldehyde acid aqueous solution for hydrothermal pretreatment, and then carrying out solid-liquid separation to obtain a solid containing glucan and lignin and a hydrolysate containing xylose; and 3) washing, drying and ball-milling the solid containing glucan and lignin, adding cellulase and a surfactant, carrying out enzymolysis, and carrying out solid-liquid separation to obtain a solid containing lignin and an enzymatic hydrolysate containing fermentable sugar. Directional removal of hemicellulose in the forest biomass and efficient enzymolysis conversion of cellulose into fermentable sugar are achieved through a chemical-physical synergistic mechanism, and the method has the advantages of being good in raw material adaptability, easy and convenient to operate, single in reagent, environmentally friendly and the like and has wide industrial application prospects in the field of biomass refining.

The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

The application discloses a method for pretreating Sargassum biomass by ultrasonic waves combined with sodium sulfite, which uses ultrasonic waves combined with sodium sulfite solution to pretreat Sargassum biomass raw materials; when the ultrasonic waves propagate in the biomass, high-intensity acoustic vibrations are generated, which can cause collisions and friction between the biomass particles, thereby breaking the cell wall and cellulose structure, and being beneficial to the sodium sulfite group to attack the aliphatic side chain of lignin and replace the hydroxyl functional group. The pretreatment of the biomass by the sodium sulfite can remove the lignin and hemicellulose in the Sargassum biomass, and at the same time, make the biomass structure become more porous and loose, reduce the mechanical strength, and increase the accessibility of cellulase. High-concentration fermentable sugar is obtained through the hydrolysis of cellulose-decomposing enzymes. In addition, a buffer solution is selected for the enzymolysis process, because it has better biocompatibility, reduces the potential toxicity of the system to the enzyme, and at the same time, as an organic acid, is easy to biodegrade and has little influence on the environment.

The invention discloses a special silage composite additive for pennisetum sinese and an application method of the special silage composite additive, and belongs to the technical field of feed silage and micro-ecological regulation and control. The compound additive comprises food-grade or feed-grade organic acid, green juice fermentation liquor and a fermentable sugar source, and preferably comprises malic acid, green juice fermentation liquor and molasses. Based on the mass fraction of the fresh pennisetum sinese to be ensiled, the content of the organic acid is 0.1%-1.5%, the content of the green juice fermentation liquor is 0.3%-5.0%, and the content of the fermentable sugar source is 0.3%-5.0%. The green juice fermentation liquor is obtained by adding a fermentable sugar source into fresh pennisetum sinese juice filtrate and then carrying out anaerobic fermentation. The composite additive and chopped pennisetum sinese raw materials are uniformly mixed and are subjected to sealed anaerobic fermentation, so that early acidification of silage can be promoted, lactic acid accumulation can be improved, the proportion of ammoniacal nitrogen can be reduced, generation of decay metabolites can be inhibited, and the fermentation quality and storage stability of the pennisetum sinese silage can be improved. The invention has great significance in the field of feed silage.