59 results about "Fermentable sugar" patented technology

The invention discloses a method for preparing fermentable sugar from recyclable low-concentration p-toluenesulfonic acid pretreated lignocellulose, which comprises the following steps: firstly, pretreating lignocellulose by using a low-concentration p-toluenesulfonic acid aqueous solution, and carrying out solid-liquid separation to obtain hemicellulose hydrolysate and a pretreated solid component; then saccharifying and pretreating the solid component by using cellulase to obtain hydrolysate rich in glucose, adsorbing the hemicellulose hydrolysate by using hypercrosslinked adsorption resin, carrying out solid-liquid separation to obtain hydrolysate rich in xylose, and carrying out desorption treatment on the resin by using industrial ethanol to obtain xylose; and performing rotary evaporation on the desorbed liquid to remove the industrial ethanol to obtain p-toluenesulfonic acid. According to the method, hemicellulose is efficiently degraded to obtain derived sugar of hemicellulose, meanwhile, the saccharification efficiency of cellulase is improved, p-toluenesulfonic acid is recycled through the hypercrosslinked adsorption resin, and the method has the advantages of being high in fermentable sugar yield, recyclable in acid catalyst, low in cost, environmentally friendly and the like.

A method for lignocellulose hydrolysis is provided. The method includes: crushing an agroforestry biomass feedstock, extracting with a toluene-ethanol solution, and drying to obtain a de-extracted feedstock; weighing a metal salt and glycerol for mixing to obtain a type IV deep eutectic solvent; weighing the de-extracted feedstock and adding into the type IV deep eutectic solvent, and reacting for 2 h-4 h at a temperature of 80° C.-120° C. and a stirring speed of 300 rpm-500 rpm to obtain a pretreatment mixture; performing a solid-liquid separation to the pretreatment mixture to obtain a filter residue and a filtrate, and then drying the filter residue after washing to obtain a lignocellulose residue after pretreatment; and performing enzymatic hydrolysis reaction to the lignocellulose residue to obtain fermentable sugar.

The invention relates to the technical field of wine brewing, and discloses a bamboo leaf powder added distilled liquor and a preparation method thereof, and the distilled liquor is prepared from 75-85 parts of grain powder and 15-25 parts of enzyme-deactivated bamboo leaf powder. The preparation method comprises the following steps: mixing the raw materials to obtain a dry mixture; adding a compound enzyme preparation containing cellulase and pectinase, preserving heat at 50-55 DEG C, performing enzymolysis and moistening, destroying a fiber structure and converting fermentable sugar; cooking the moistened material, and adding a saccharifying enzyme for saccharifying; primary fermentation is performed at 28-32 DEG C, and then secondary fermentation is performed at 22-25 DEG C; during distillation, foreshot with the expected wine yield of 1.5%-2.0% is intercepted and abandoned to remove methanol, fractions with the volume of 50% or above are collected to obtain raw wine, and the wine is obtained through ageing. The raw material utilization rate and the wine yield are improved through enzymolysis, the methanol content is controlled through temperature-variable fermentation and precise cut distillation, the raw and green taste of bamboo leaves is eliminated, and the finished wine has the unique composite style of bamboo fragrance and grain fragrance.

The invention relates to a protein feed prepared from biologically refined distiller's grains and a preparation method thereof, and relates to the technical field of bioengineering and feed resource development. The method comprises the following steps: (1) sequentially carrying out acid pretreatment and double-enzyme synergistic hydrolysis on the white spirit vinasse to obtain hydrolysate containing fermentable sugar; (2) adding a candida utilis yeast liquid and urea, and carrying out carbon-nitrogen synergistic liquid fermentation to obtain a fermentation liquid with the viable count of candida utilis greater than or equal to 4.0 * 10 < 8 > CFU / mL; and drying. According to the method, the white spirit vinasse is subjected to diluted acid high-pressure pretreatment and double-enzyme synergistic hydrolysis, then candida utilis is utilized for carbon-nitrogen synergistic liquid fermentation, efficient utilization of glucose and xylose is achieved, and the mycoprotein yield is larger than or equal to 0.70 g / L / h; according to the method, all-component high-value utilization of the distiller's grains is realized, and a process path with industrial production potential is provided for preparing the protein feed by biorefining the distiller's grains.

A low-cost process is provided to render lignocellulosic biomass accessible to cellulase enzymes, to produce fermentable sugars. Some variations provide a process to produce ethanol from lignocellulosic biomass (such as sugarcane bagasse or corn stover), comprising introducing a lignocellulosic biomass feedstock to a single-stage digestor; exposing the feedstock to a reaction solution comprising steam or liquid hot water within the digestor, to solubilize the hemicellulose in a liquid phase and to provide a cellulose-rich solid phase; refining the cellulose-rich solid phase, together with the liquid phase, in a mechanical refiner, thereby providing a mixture of refined cellulose-rich solids and the liquid phase; enzymatically hydrolyzing the mixture in a hydrolysis reactor with cellulase enzymes, to generate fermentable sugars; and fermenting the fermentable sugars to produce ethanol. Many alternative process configurations are described. The disclosed processes may be employed for other fermentation products.

This invention relates to a protein feed prepared from biorefined baijiu lees and its preparation method, belonging to the fields of bioengineering and feed resource development technology. It includes the following steps: (1) subjecting the baijiu lees to acid pretreatment and dual-enzyme synergistic hydrolysis sequentially to obtain a hydrolysate containing fermentable sugars; (2) adding Candida utilis culture and urea for carbon-nitrogen synergistic liquid fermentation to obtain a Candida utilis viable count ≥4.0 × 10⁻⁶. 8 Fermentation broth with CFU / mL; drying is then performed. This invention pretreats baijiu (Chinese liquor) lees under high pressure with dilute acid, followed by synergistic hydrolysis with two enzymes, and then utilizes *Candida utilis* for carbon-nitrogen synergistic liquid fermentation, achieving efficient utilization of glucose and xylose, with a cell protein yield ≥0.70 g / L / h. This invention realizes the high-value utilization of all components of baijiu lees, providing a process route with industrial-scale production potential for the biorefining of baijiu lees to prepare protein feed.

The present invention produces fermented kombucha beverages with reduced alcohol content by utilizing allulose or analogues thereof as the primary fermentable sugar. These rare sugars limit ethanol production during fermentation while preserving flavor, probiotic benefits, and acidity. The invention further encompasses the incorporation of Kratom (Mitragyna speciosa) extracts or alkaloids into the fermentation process or final product, enabling a low-alcohol kombucha with potential enhanced wellness properties. The resulting beverage maintains an alcohol content below 0.5% by volume, making it suitable for non-alcoholic labeling and broader consumer appeal. The invention is preferably a bottled beverage.

The present invention discloses a method for preparing fuel ethanol from straw biomass and the fuel ethanol, belonging to the technical field of fuel ethanol production. It includes: dissolving and hydrolyzing straw and concentrated sulfuric acid to obtain a saccharified solution, separating out a sugar-rich solution in a simulated moving bed chromatography system, and then mixing it with Saccharomyces cerevisiae SEB3 for fermentation, and generating fuel ethanol through distillation. The method for preparing fuel ethanol from straw biomass provided by the present invention can obtain fermentable sugars under normal pressure and low temperature conditions by using concentrated acid hydrolysis, and has the remarkable advantages of low energy consumption, low toxic by-products, and no need to input cellulase. And Saccharomyces cerevisiae SEB3 is used for fermentation. This strain has the ability of co-fermenting all sugars, can realize the fermentation of all sugars, synchronously convert all sugars into ethanol, the sugar consumption efficiency reaches more than 90%, and the ethanol recovery rate reaches more than 80%, and it is applicable to industrial production.

The invention discloses a full-value protein biological feed production method, and relates to the technical field of protein feed production, the production method comprises the following steps: double-strain co-fermentation: cooling fermentable syrup to 38-42 DEG C, adjusting the pH value to 6.5-7.0, inoculating a mixed strain of Thermoactinomyces vulgaris and Candida utilis, fermenting for 18-24 hours under the condition that the dissolved oxygen is maintained to be 30-60%, and carrying out double-strain co-fermentation; in the process, the pH, dissolved oxygen and temperature are monitored in real time by an on-line sensor controlled by a PLC (Programmable Logic Controller), and materials are automatically supplemented; and membrane separation and concentration: enabling the fermentation liquor to pass through a PVDF (Polyvinylidene Fluoride) ultrafiltration membrane with the molecular weight cutoff of 10kDa-30kDa, and intercepting thalli and protein aggregates by a membrane concentration multiple of 5-10 to obtain a membrane concentrated solution with the protein concentration of more than or equal to 50g / L. According to the method, agricultural waste is efficiently converted into complete protein through double-bacterium synergistic fermentation, the problems that traditional single-bacterium fermentation is low in yield and unbalanced in nutrition are solved, and the protein yield is increased.

The invention relates to the technical field of microbial fermentation and bio-fertilizers, and discloses a compound microbial fertilizer prepared by treating rotten vegetable leaves with a compound culture medium and a fermentation method of the compound microbial fertilizer. 5-8 parts of a fermentable sugar source; 2-4 parts of a composite nitrogen source; 1-1.5 parts of an inorganic mineral substance; 0.5 to 0.8 part of a biological activator; 0.3 to 0.5 part of a buffering agent; the vegetable juice is prepared by mixing Chinese cabbages, tomatoes and soybeans according to the mass ratio of 2: 1: 1, and enzymolysis treatment is carried out on the Chinese cabbages, the tomatoes and the soybeans. By optimizing the proportion of the carbon source, the nitrogen source, the inorganic mineral substances and the biological activating agent, the nutrition supply capacity of the culture medium is enhanced, and meanwhile, efficient synergistic proliferation of dominant flora is achieved in combination with a multi-stage fermentation strategy. The culture medium not only can improve the growth rate and metabolic activity of microorganisms, but also can keep a stable physical and chemical environment in the fermentation process, and improves the quality and application effect of a final product.

The invention discloses a method for efficiently saccharifying brewing sorghum straws, which comprises the following steps: pretreating the brewing sorghum straws by adopting steam explosion under the conditions that the explosion pressure is 0.9-1.5 MPa, the pressure maintaining time is 4-8 minutes, the particle size of a material is 3-9 cm and the water content of the material is 30-70%; carrying out enzymolysis saccharification on the brewing sorghum straws subjected to steam explosion treatment, wherein the initial solid content is 10-20%, the enzyme addition amount is 20-50 FPU / g, the enzymolysis temperature is 45-55 DEG C, and the material particle size is 10-30 meshes; in the enzymolysis saccharification process, materials are supplemented in stages to regulate and control the solid content, the materials are supplemented at least once, and the final solid content is 35-40%. According to the method disclosed by the invention, the biomass conversion efficiency is remarkably improved through system optimization pretreatment and an enzymolysis process, and the solid content is improved and the fermentable sugar concentration is remarkably improved in combination with stepped material supplementation.

The present invention belongs to the field of microbial technology, and particularly relates to a Lactobacillus sakei room-temperature coagulated carrot yogurt and a preparation method thereof. The present invention provides a preparation method of room-temperature coagulated carrot yogurt, and the preparation method comprises the following steps: mixing carrot juice and milk evenly, adding fermentable sugar to obtain a mixed solution; homogenizing and performing high-temperature sterilization on the mixed solution, and cooling to room temperature to obtain a sterilized solution; adding a starter to the sterilized solution for fermentation to obtain room-temperature coagulated carrot yogurt. The preparation method provided by the present invention has low cost and significantly shortens the preparation time; the prepared yogurt has a good curd state, a unique flavor and a delicate taste.

The invention discloses a preparation method of a chestnut distilled liquor with fruity flavor, belonging to the field of chestnut distilled liquor brewing. The invention uses 100% chestnuts as raw materials, and through the Maillard reaction during baking, ketone and aldehyde flavor substances are generated. At the same time, a four-enzyme preparation three-stage saccharification process is adopted, generating abundant fermentable sugars and amino acids, providing substrates for yeast metabolism, and the content of flavor substances generated by fermentation metabolism is relatively high. No flavoring and flavor substances are added in the production process. The produced chestnut distilled liquor contains ethyl octanoate with brandy aroma, ethyl lactate with floral, fruity and sweet aroma, ethyl acetate which is the main flavor substance of light-flavor liquor with banana or apple aroma. At the same time, the furfural content is relatively high, and the liquor body also contains a faint soy sauce aroma. The complex flavor substances effectively enrich the flavor of the chestnut distilled liquor, making up for the defect of insufficient aroma of distilled liquor made from a single raw material, having the unique elegant and harmonious fruity and floral aroma of chestnuts and a strong liquor aroma, and at the same time also containing a faint soy sauce aroma.

The invention is suitable for traditional Chinese medicine residue resource utilization and biological refinery technology, and provides a preparation method of a high-protein and high-fat feed. The method comprises the following steps: adding a certain amount of cheap nitrogen source into plant source wet traditional Chinese medicine residues rich in starch to prepare a fermentation culture medium, inoculating trichoderma reesei and lipomyces starkeyi for mixed culture, and performing combined action on cellulase produced by the trichoderma reesei and amylase produced by the lipomyces starkeyi on the traditional Chinese medicine residues, so as to obtain a fermentation product. And rapidly degrading into fermentable sugar which is efficiently used for microbial growth and grease and protein production, then carrying out solid-liquid separation, and drying the solid to obtain the high-protein and high-grease feed. Wet traditional Chinese medicine residues rich in starch are used as raw materials, cellulase and amylase production, enzyme hydrolysis and fermentation are highly integrated, the number of equipment is reduced, the production and processing flow is simplified, and the production cost of the high-protein feed is remarkably reduced. The fermented product serving as the feed is rich in protein and microbial oil, the fatty acid composition of the microbial oil is similar to that of vegetable oil, the nutritional ingredients of the feed are further improved, and rapid growth of poultry can be efficiently promoted. The method can promote the resource utilization of the cheap traditional Chinese medicine residues, provides a low-cost new method for improving the feed quality, and has remarkable economic benefits.

The invention discloses an Actinomyces sp. Strain, a separation and purification method and application of the Actinomyces sp. Strain in straw degradation, the Actinomyces sp. Strain is Actinomyces sp. And has been preserved in the China General Microbiological Culture Collection Center on July 3, 2025, and the preservation number of the Actinomyces sp. Strain is CGMCC No. 46460. As facultative anaerobic bacteria, the Actinomycete disclosed by the invention can be used for realizing efficient degradation under an anaerobic or aerobic condition in a single system, so that the process is remarkably simplified. In the practical application process, the strain can be used as a functional strain to be added into straw compost, organic matter decomposition is promoted, the composting period is shortened, and meanwhile the humus content is increased; fermentable sugar is generated by pretreating straws and is used for producing bioethanol or biogas; and the degraded straw residues can enhance the water-retaining property and microbial activity of the soil.

The invention relates to the technical field of automatic control, in particular to a low-sugar fruit juice production method based on enzyme catalysis dynamic control, which comprises the following steps: step 1, synchronously acting multiple varieties of fruit pulp with pre-enzyme activity factors under a controlled gas-liquid equilibrium replacement condition to obtain homogenized pre-enzyme pulp; step 2, enabling the homogenized pre-enzymatic fruit pulp to form a steady-state multi-interface layer in an alternate pulse ventilation and rotating film pressurization mode, so as to obtain prefabricated fruit pulp; 3, based on the real-time online detection data of the prefabricated fruit pulp, calling a dynamic load segmented enzyme quantity regulation and control algorithm to generate a fruit pulp fermentable sugar content predicted value sequence so as to realize non-linear deceleration reduction of the fermentable sugar content, and obtaining a fruit pulp product; and 4, carrying out instantaneous cold pressing on the fruit pulp product to form semi-permeable liquid layer composite particles, and completing one-time packaging, so as to obtain the stable low-sugar fruit juice finished product. Under the condition of not adding an external sweetening agent, the sugar content control, the flavor quality and the nutrition retention are considered.

A method is disclosed for providing a c5 / c6 product from a lignocellulosic material, said method comprising the steps of: (i) pretreatment of the lignocellulosic material; (ii) solid / liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction; (iii) enzymatic hydrolysis of the fiber from said first solid fraction from step (b) using an enzyme composition capable of degrading lignocellulosic material, thereby providing a c5 / c6 fiber paste comprising c5 and / or c6 sugars; (iv) solid / liquid separation of the c5 / c6 fiber paste from step (c) into a second solid fraction and a second liquid fraction; and optionally (v) combining said first liquid fraction and said second liquid fraction for enzymatic hydrolysis of mixed sugar (msh), thereby providing a c5 / c6 msh product.

The gynura formosana fermentation equipment comprises a fermentation tank, a support is rotationally connected to the fermentation tank, a mounting table is fixedly mounted on one side of the support, a driving assembly is mounted on the mounting table, the output end of the driving assembly is connected with one end of the fermentation tank to drive the fermentation tank to rotate, and the fermentation tank comprises a tank body; a stirring assembly is mounted in the tank body. The gynura formosana fermentation equipment has the advantage of efficient mixing, and the problems that in the using process of existing gynura formosana fermentation equipment, gynura formosana raw materials and lactic acid bacteria strains are inconvenient to efficiently mix, lactic acid bacteria cannot make uniform contact with fermentable sugar on the surfaces of gynura formosana cut sections, strains in part of areas cannot grow in time, and the fermentation effect is poor are solved. And local concentration nonuniformity of metabolites can be caused.

The invention discloses a deep-eutectic solvent-based electrolyte containing Lewis acid, which is characterized in that the electrolyte comprises deep-eutectic solvent and Lewis acid, the deep-eutectic solvent is choline chloride and ethylene glycol, and the Lewis acid accounts for 0.0-1.0% of the mass of the deep-eutectic solvent. The invention further discloses the eutectic solvent-based electrolyte containing the Lewis acid and the method for deconstructing the lignocellulose biomass at the normal temperature by using the eutectic solvent-based electrolyte. The conditions are mild, the selectivity is high, and the method is environment-friendly; in addition, the obtained hemicellulose hydrolysate is rich in fermentable sugar (xylose, glucose and the like) and can be directly used for producing value-added products through microbial fermentation, and full-component utilization of lignocellulose is achieved.

A method for producing bio-oil using cellulosic or lignocellulosic biomass as input is provided. Microwave hydrolysis of cellulosic or lignocellulosic biomass is used as a pretreatment process to extract fermentable sugars from the cellulosic or lignocellulosic biomass. The oleaginous yeast is fermented using the extracted fermentable sugars to produce the bio-oil.

The present application relates to a method for producing astaxanthin by using lignocellulosic raw materials, which comprises: sequentially carrying out steam explosion treatment, enzymatic hydrolysis treatment and fermentation treatment on the lignocellulosic raw materials to produce astaxanthin; and the fermentation bacteria used in the fermentation treatment include red Phaffia and a gene engineered Escherichia coli for producing pyruvic acid. The method for producing astaxanthin according to the present application is characterized in that after the lignocellulosic raw materials are subjected to steam explosion treatment, the hemicellulose is partially degraded, which is beneficial to subsequent enzymatic catalysis or microbial utilization, then enzymatic hydrolysis is carried out to form a high-concentration fermentable sugar solution mixed with five-carbon sugars and six-carbon sugars, and finally fermentation treatment is carried out by using the fermentable sugar as a carbon source. In order to improve the astaxanthin synthesis capacity of the red Phaffia, the present application creatively adopts a co-fermentation mode of the red Phaffia and the Escherichia coli, and uses the pyruvic acid synthesized by the Escherichia coli as a promoter to strengthen the astaxanthin synthesis of the red Phaffia, so that the astaxanthin yield is significantly improved.

The invention provides a method for extracting alcohol by using a by-product after garlic oil production, which comprises a pretreatment step, a fermentation step, a distillation step and a refining step, and through the combination of crushing and high-temperature cooking, starch in the garlic by-product is fully gelatinized, the cell wall structure is destroyed, and the alcohol content is reduced; more importantly, infectious microbes possibly carried in the raw materials are effectively killed, and a solid foundation is laid for subsequent pure fermentation; amylase and saccharifying enzyme are selectively added for enzymolysis, so that macromolecular carbohydrates are further accurately degraded into fermentable sugars which can be directly utilized by yeast, the saccharification rate of the raw materials is remarkably improved, and a core guarantee is directly provided for improving the final ethanol yield. Through combination of normal pressure rectification and pressurized rectification, ethanol is separated and concentrated, and through deep processing units such as molecular sieve dehydration or blending and aging, diversified alcohol products from industrial fuel and medical disinfection to high-end drinking can be flexibly produced by the same basic process, so that the application range and the economic value are expanded.

This invention discloses a method for promoting the hydrolysis of lignocellulose into fermentable sugars, belonging to the field of biorefining technology. The method includes the following steps: (1) adding ammonium sulfate to the lignocellulose raw material, then mixing it with a glycerol-water mixture, and pretreating it at a certain temperature and time to destroy the dense structure of the lignocellulose; (2) adding ammonium sulfate and cellulase to the pretreated system, and carrying out a saccharification reaction under suitable conditions to convert it into fermentable sugars. This invention solves the problems of high energy consumption, high pollution, and low saccharification and enzymatic hydrolysis efficiency of traditional pretreatment by leveraging the solubilizing, crystallinity-reducing, and biocatalyst-protecting effects of glycerol and the synergistic effect of ammonium sulfate. Compared with traditional hot water pretreatment, the yield of fermentable sugars is significantly increased, and the pretreatment process does not require strong acids or bases; glycerol can be recycled and reused, significantly reducing production costs and possessing significant industrial application value.

The invention relates to a method for separating and purifying fermentable sugar from enzymatic hydrolysate, and belongs to the technical field of biochemical engineering. The method for separating and purifying the fermentable sugar from the enzymatic hydrolysate comprises the following steps: S1, filtering and concentrating the enzymatic hydrolysate to obtain a mixed sugar solution; s2, uniformly mixing the mixed sugar solution obtained in S1 with a flocculating agent solution (a flocculating agent is a cationic flocculating agent), performing flocculation, and removing precipitate to obtain a sugar solution I; s3, sequentially carrying out activated carbon adsorption treatment and diatomite adsorption treatment on the sugar solution I obtained in S2 to obtain a sugar solution II; s4, the sugar solution II obtained in S3 is sequentially subjected to cation exchange resin adsorption treatment and anion exchange resin adsorption treatment, and fermentable sugar is obtained. According to the method, substances for inhibiting microbial growth and metabolism in the enzymatic hydrolysate of the cellulose biomass material can be effectively removed, and high-quality fermentable sugar can be obtained.

A method for efficiently hydrolyzing lignocellulosic feedstocks in the presence of additives. The present disclosure relates to an improved method for enzymatically hydrolyzing a lignocellulosic feedstock, comprising the steps of impregnating a lignocellulosic feedstock to obtain an impregnated slurry, adjusting the concentration and pH of the treated slurry to obtain a stream, adding an additive to the stream to obtain an additive-treated stream, and enzymatically hydrolyzing the additive-treated stream to obtain an enzymatically hydrolyzed stream containing fermentable sugars. The present disclosure also discloses a step of co-fermenting the enzymatically hydrolyzed stream to obtain a fermentation product. The improved method for enzymatically hydrolyzing a lignocellulosic feedstock of the present invention is characterized in that the addition of an additive to the stream increases the efficiency of enzymatic hydrolysis by at least 7%.

The invention belongs to the technical field of biomass resource utilization, and particularly relates to a preparation method of corn straw fermentable sugar based on hot alkali pretreatment and enzymolysis coupling, which comprises the following steps: (a) hot alkali pretreatment: mixing crushed corn straw with an alkali solution, and treating at high temperature; (b) washing and detoxification: washing the solid residues obtained after pretreatment in the step (a) for several times; (c) enzymolysis: adding the residues washed in the step (b) into a buffer solution, then adding cellulase, and carrying out oscillatory reaction to prepare the corn straw fermentable sugar. Compared with the prior art, the method disclosed by the invention solves the problems of insufficient / excessive pretreatment, high detoxification water consumption or extensive control and low enzymolysis sugar production efficiency in the prior art. According to the scheme, the stable high saccharification efficiency is realized on the premise of ensuring the low threshold value of the inhibitor and the controlled water consumption / energy consumption.

The invention discloses a preparation method of corn vinegar through lactic acid bacteria synergistic fermentation in combination with ultrasonic-microwave accelerated aging treatment, the method adopts an ultrasonic-microwave accelerated aging treatment technology to prepare novel corn vinegar, corn is taken as a raw material, liquefaction and saccharification stages are optimized through a two-enzyme method, and efficient conversion from corn starch to fermentable sugar is realized; lactobacillus plantarum is used for synergistically performing alcoholic fermentation to generate a plurality of flavor substances such as lactic acid and ethyl lactate; and the aging process of the corn vinegar is accelerated by combining ultrasonic-microwave aging accelerating treatment. Wherein ultrasonic waves can initiate a local high-temperature and high-pressure effect and a medium cavitation effect, so that polar molecules are orderly arranged, the molecular activation energy is improved, reactions such as esterification, condensation and oxidation reduction are accelerated, the energy consumption is relatively low, and the green production requirement is met; the microwave treatment breaks the association state of molecular groups in the original system through a microwave generator, and further accelerates the alkyd esterification process. The method solves the problems of unstable components and inharmonious smell in newly brewed corn vinegar.

A method is described for increasing the amount of fermentable sugars in a fermentation substrate by treating with a combination of an enzyme having transglucosidase activity and an enzyme having glucoamylase activity to hydrolyze oligosaccharides and / or polysaccharides that are typically unhydrolyzable by glucoamylases alone during fermentation. The method is most effective for fermentation substrates containing small amounts of maltose and maltotriose.