196 results about "Galactose" patented technology

The invention provides a method for separating and purifying galactose and glucose by using a simulated moving bed chromatography system, and relates to the technical field of saccharide separation and purification. According to the method, the simulated moving bed chromatography system is used for separating and purifying the galactose, operation can be carried out under the set temperature condition, the separation and purification efficiency of the galactose is remarkably improved, continuous large-scale efficient separation and purification of the galactose can be achieved, the separation and purification effect is good, the yield is high, and the product quality is good. According to the method, pure water is used as an eluent, and analyzed and purified compounds are free of damage, low in toxicity, rich in source and low in cost.

The invention discloses a preparation method of soybean milk powder for promoting immune regulation, and belongs to the technical field of soybean product processing. Performing biological enzymolysis; modifying soybean protein; compounding functional components; and preparing the microcapsule. An ultrasonic-enzyme composite extraction technology is applied, and the enrichment rate of components with immunocompetence is increased. According to the method, the Maillard reaction is performed on the soybean protein and the polysaccharide by utilizing an ultrasonic-assisted heating technology, so that the emulsifying characteristic of the soybean protein isolate is improved, and the immunoregulation promoting performance of the soybean protein isolate is greatly improved. According to the invention, through a microcapsule embedding technology, glycosylated soybean protein is wrapped in the particles by a wall material formed by galactooligosaccharide and resistant dextrin, the stability and bioavailability are improved, the microencapsulation efficiency is greater than 95%, and the immunoregulation promotion performance of the microencapsulated soybean protein is effectively exerted. By adopting spray drying, inactivation of immune active components caused by high temperature is avoided.

The application discloses a Dendrobium nobile Lindl polysaccharide component group, a preparation method thereof and application of the Dendrobium nobile Lindl polysaccharide component group in immunoregulation. Four polysaccharide components with definite structures, namely DNP-0-1 (>100 kDa), DNP-0-2 (30-100 kDa), DNP-0-3 (<30 kDa) and DNP-1-1 (<50 kDa), are synchronously separated from Dendrobium nobile Lindl by combining ultrasonic extraction process optimized by a response surface method with DEAE-cellulose column chromatography and ultrafiltration membrane series grading technology. After structure characterization and in-vitro immunological activity evaluation, the four components all have immunoregulatory activity, and the activity of DNP-0-1 is optimal. Pearson correlation analysis shows that the content of galactose and the molecular weight are extremely significantly positively correlated with the immunological activity, and the content of glucose and mannose is significantly negatively correlated with the activity. The application establishes an integrated research system of 'preparation by grading-structure characterization-activity screening-structure-activity analysis', and provides a material basis and evaluation basis for the development of immunoregulatory drugs or functional foods.

Methods and compositions use a galactooligosaccharide (GOS) for increasing absorption of zinc, such as zinc naturally present in ingredients of the composition and / or present in a food product, a meal or a diet, wherein the food product, meal or diet is combined with the composition and / or consumed separately from the composition. Some embodiments are directed to a milk-based composition comprising zinc intrinsically present in the milk, further comprising milk protein, and further comprising a mixture of lactose, glucose, galactose and the galactooligosaccharide (GOS). In some embodiments, the milk-based composition does not contain exogeneous zinc, such that the only zinc in the composition is the zinc intrinsically present in the milk.

The application discloses a ginseng polysaccharide, a preparation method and application, and the ginseng polysaccharide is ginseng polysaccharide GP60, which is composed of glucose, galactose and arabinose, and the molar ratio of each monosaccharide is glucose: galactose: arabinose = 77.1: 16.8: 6.1; a new preparation method is adopted; after soaking and twice decocting, different proportions of 95% ethanol are added into the filtrate in batches; the obtained precipitate is added with water to prepare a solution with a relative density of 1.1 g / ml; a certain amount of the solution is taken, a certain proportion of chloroform and n-butanol is added, and a polysaccharide aqueous solution is prepared; the polysaccharide aqueous solution is added with 95% ethanol until the alcohol content reaches 60%; the solution is statically placed for 12 hours; centrifugal separation is carried out; the precipitate is collected; the collected precipitate is sequentially washed with 95% ethanol, anhydrous ethanol, isopropyl alcohol and ether; the solvent is volatilized; and the ginseng polysaccharide GP60 is obtained. The ginseng polysaccharide GP60 is non-toxic, has the effect of preventing light aging, and can be widely applied in the fields of food or health food, anti-light aging skin care products, washing and caring products and anti-light aging medicines.

This invention relates to the use of a natural terpene compound in pharmaceuticals, said natural terpene compound having the structure shown in formula (I), and having the function of preparing a compound for the prevention or treatment of hypoxia-inducible factor-1. α Use in medicines for diseases associated with abnormally elevated activity. This invention has discovered that the compound possesses hypoxia-inducible factor-1. α It exhibits inhibitory activity, effectively suppressing macrophage inflammatory responses and hepatocyte damage. It also demonstrates significant protective function against lipopolysaccharide / galactosamine-induced acute liver injury in mice, and can be used as a lead compound for the preparation of new drugs or drugs for the prevention and treatment of acute liver injury.

The application discloses a group of antibodies specifically recognizing IgA1 with galactose deficiency and related biological materials and application thereof, and belongs to the technical field of antibodies. The main problem to be solved by the application is how to obtain anti-Gd-IgA1 antibodies with high specificity. In order to solve the above technical problem, the application provides antibodies or antigen binding fragments thereof, wherein the antibodies are antibody B0032, antibody B0027, antibody B0025 or antibody B0041. Compared with the existing antibody KM55, the obtained antibodies have higher affinity with antigens, the diagnostic sensitivity and specificity of IgA nephropathy are improved, and the antibodies are expected to be more effectively used for the diagnosis of IgA nephropathy, monitoring of treatment efficacy, judgment of prognosis and the like.

The invention discloses a method for improving yield of anthocyanin extracted by an enzymic method, according to the method, phospholipase is added and used to act on a cell membrane structure, so that the extraction rate of the anthocyanin and the extraction rate of galactoside in the anthocyanin are obviously improved, and the extraction effect of the anthocyanin is greatly improved through a three-stage enzymolysis system. The total enzymolysis extraction time is within 4 h, and compared with 6-7 h of a traditional enzymatic extraction process, the production period is greatly shortened.

The invention provides high-activity crataegus pinnatifida fruit vinegar and a preparation method thereof.The preparation method comprises the steps that crataegus pinnatifida fruits are juiced, then a composite chelating agent composed of polyglucuronic acid and monopotassium phosphate is added for chelating treatment, and a polysaccharide-metal ion cross-linked network is removed; then adding endo-beta-1, 4-glucanase to carry out linear polysaccharide directional enzymolysis, so that the viscosity of the fruit juice is obviously reduced; after the components are adjusted, double modified yeast beta-glucan microspheres are added, yeast is inoculated for alcoholic fermentation, the microspheres are subjected to double grafting modification through galactooligosaccharide and theaflavin, and active components in the crabapple can be directionally captured and stabilized through hydrophilic and hydrophobic effects respectively; after alcohol fermentation is finished, acetic bacteria are inoculated for acetic fermentation, and finally, filtering and sterilization are performed. According to the method disclosed by the invention, by virtue of a synergistic strategy of breaking a network firstly, then carrying out enzymolysis debonding and then carrying out microsphere loading, the fermentation mass transfer efficiency is effectively improved, and meanwhile, the retention rate of functional components such as polyphenol and saponin in the fruit vinegar is increased.

The present application provides a kind of planting method of reducing allelochemicals and improving polysaccharide content of Rhizoma Polygonati, belongs to plant cultivation technical field.The planting method is planted in acidic sandy loam soil, and "biochar C" is applied to the soil 1-2 times in September-October of the second and third years, and the fruits mature, the stems are laid down after the third year, and then it can be harvested."Biochar C" is a modified, loaded with salicylic acid and UDP-D-galactose biochar.The present application not only helps to improve the physicochemical properties and bacterial community diversity of Rhizoma Polygonati planting soil, thereby reducing the allelochemicals content of Rhizoma Polygonati rhizome, but also can improve the polysaccharide content of rhizome, which helps to improve the quality of Rhizoma Polygonati and enhance its efficacy.

The present invention relates to a composition for preventing or treating lupus or glomerulonephritis comprising a recombinant stabilized Galectin-9 protein. Specifically, the recombinant stabilized Galectin-9 protein of the present invention has been confirmed to exhibit safety in a systemic lupus erythematosus (SLE) animal model, reduce skin lesions, lymphadenopathy, and proteinuria caused by lupus, ameliorate lupus nephritis and glomerulonephritis, and decrease the concentration of anti-dsDNA antibodies in plasma. Accordingly, the recombinant stabilized Galectin-9 protein of the present invention can be effectively used as an active ingredient in a composition for preventing or treating lupus or glomerulonephritis.

The present application relates to a kind of brain health nutrition capsule and its preparation method, belong to the technical field of functional health food.The preparation method includes the following steps: taking walnut kernel powder, DHA algal oil microcapsule powder, phosphatidylserine, ginkgo biloba extract and oligosaccharides are mixed, soft material is prepared by adding purified water, granulation is extruded by screen, and granule core is obtained by drying;Granule core is coated, and brain nutrition core granule is obtained by drying;Prepare base nutrition layer granule;Brain nutrition core granule and base nutrition layer granule are mixed, and composite granule is obtained, and composite granule is filled into capsule.The present application not only solves the three major obstacles of oxidation, precipitation and vitamin degradation of high polyphenol walnut base formula in gastric acid environment, but also unexpectedly builds from stomach protection to intestinal microecological optimization, finally realizes the cascade effect of brain nutrition efficient absorption, meets the core needs of teenagers brain health and intelligence, and truly realizes the effect of brain health and intelligence.

[Problem] To provide a composition for regulating blood pressure for a healthy person. [Solution] A blood-pressure-regulating composition for a healthy person, said composition being characterized by containing a guar gum degradation product, and being characterized in that: 70 mass% or more of the guar gum degradation product has an average molecular weight within the average molecular weight range of 1.0 × 103 to 1.0 × 105; the composition has a viscosity of 10 mPa·s or less when a 2 mass% aqueous solution at 37°C is measured at 10 rpm using an E-type viscometer, or has a viscosity of 5 mPa·s or less when the aqueous solution is measured at 20 rpm, or has a viscosity of 3 mPa·s or less when the aqueous solution is measured at 30 rpm; the composition is obtained by hydrolyzing a galactomannan polysaccharide which contains guar-derived endosperm and in which the content ratio (galactose:mannose) of galactose and mannose is within the range of 1:1.3 to 1:2, by using a microorganism-derived β-mannanase to reduce the molecular weight of the polysaccharide; and the composition contains at least 70 mass% of dietary fiber in terms of the dietary fiber content stipulated in the enzyme-HPLC method.

The invention relates to a method for improving PD-1 titer produced by Chinese hamster ovary cells. The method comprises the following steps: inoculating the Chinese hamster ovary cells into an initial culture medium for culturing; after the culture is performed for 48 hours, feeding a fed-batch culture medium every day, and maintaining the glucose concentration in the culture medium to be 1.3-1.7 g / L, the galactose concentration to be 0.6-1.0 g / L and the L-sodium lactate concentration to be 14-16 mM; the culture temperature is reduced, a fed-batch culture medium is fed every day, the glucose concentration in the culture medium is maintained at (2-2.8) g / L, the galactose concentration is maintained at (1.7-2.2) g / L, and cells are harvested. Galactose is introduced to serve as a second carbon source, and control over the culture initial metabolism environment and the carbon source in the culture stage is combined, so that the maximum accumulation amount of lactic acid is obviously reduced, the cell culture period is remarkably prolonged, and the maximum titer of the PD-1 antibody is remarkably improved compared with an existing process.

The invention relates to the field of food processing, and particularly discloses a bread fermentation accelerant and application thereof in bread baking. The bread fermentation accelerant comprises isomaltooligosacharide, galactooligosaccharide and fructo-oligosaccharide in a mass ratio of 5: (2-4): (1-2). The bread fermentation accelerant has the advantages that the bread fermentation accelerant can be used for bread with the high dietary fiber content, the fermentation capacity of the bread with the high dietary fiber content is improved, the specific volume of the bread is increased, and the bread is full and complete in appearance, soft and elastic in texture, spongy in tissue, uniform in air hole, good in ductility, golden in color and luster, fragrant, soft and delicious.

The present invention relates to a nutritional composition for infants and young children comprising a digestible carbohydrate component wherein the digestible carbohydrate component comprises a mixture of free galactose and at least one digestible carbohydrate selected from the group consisting of maltodextrin and glucose syrup, and with reduced or no lactose and glucose. The invention also relates to the use of a nutritional composition according to the invention for improving the body composition or the development of the body composition.

The invention discloses asterias amurensis glucan as well as a preparation method and application thereof, and belongs to the technical field of biological medicine, the asterias amurensis glucan is obtained by combining an enzymolysis method with anion exchange column chromatography and gel column chromatography purification, the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose, and the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose. A terminal alpha-D-glucose branch chain is arranged at the O-6 position of a main chain, and meanwhile, a small amount of mannose and galactose are contained. Moreover, it is proved for the first time that the asterias amurensis glucan can serve as an intestinal barrier protective agent, and oxidative damage, caused by H2O2, of intestinal epithelial cells is improved by up-regulating an Nrf2 signal channel and tight junction protein. Therefore, the asterias amurensis glucan prepared by the invention has a huge potential of being developed into medicines and health-care products for protecting intestinal tracts.

The invention belongs to the technical field of microbial engineering, and particularly relates to a composite yeast agent and application thereof. The composite yeast agent is composed of a saccharomyces cerevisiae liquid inoculant, a geotrichum galactose yeast liquid inoculant and a Lodderomyces elongisporus liquid inoculant according to a volume ratio of 1: 1: 1. The invention provides a composite yeast agent which can effectively improve the content of flavor substances, particularly the content of esters and the composition of rich esters in a wine body when being used for brewing rice wine, and particularly, the method can enrich the ester content and the composition of the esters in the rice wine, and the ester content and the composition of the esters in the rice wine are obviously improved compared with a control group. And the produced wine body has rich and harmonious rice fragrance, prominent ester fragrance, mellow and soft sweet taste and prominent style, and has important application value for improving the quality of the rice wine.

Methods and compositions for treating and / or preventing gastrointestinal disorders and / or improving digestive comfort in an individual in need thereof, the composition comprising a low sugar, low lactose, milk-based composition comprising galactooligosaccharide (GOS).

The objective of this invention is to provide a means for controlling the proliferation of *Streptobacter* spp. A composition for controlling the proliferation of *Streptobacter* spp. is provided, comprising any one selected from kohlbiose and oligosaccharides with kohlbiose as a constituent sugar. In one embodiment, the composition comprises galactosylkohlbiose as any one selected from kohlbiose and oligosaccharides with kohlbiose as a constituent sugar.

This invention discloses a preparation process for a homogeneous immunomodulatory polysaccharide from *Gynostemma pentaphyllum* and its application in the preparation of anti-melanoma drugs and immunomodulators, relating to the field of pharmaceutical manufacturing technology. The preparation process includes four steps: compound enzyme-ultrasound synergistic extraction, fractional alcohol precipitation to remove proteins, DEAE-52 ion exchange column chromatography, and Sephacryl S-400 gel permeation chromatography purification. The resulting homogeneous polysaccharide (GOP-3) has a weight-average molecular weight of 12±1 kDa and is composed of mannose, glucose, and galactose in a specific ratio. The GOP-3 of this invention has a weight-average molecular weight (Mw) of 12.8 kDa, a dispersion (Mw / Mn) < 1.2, a uronic acid content of 23.5%, a well-defined structure, and is suitable for drug application. Furthermore, GOP-3 can significantly promote the secretion of IL-12 by dendritic cells and induce the differentiation of naive T cells into Th1 cells. Simultaneously, compared with a full-dose PD-1 monoclonal antibody, the combination of GOP-3 and a half-dose monoclonal antibody not only reduces the risk of immune-mediated pneumonia caused by antibody drugs but also increases the tumor inhibition rate by more than 40%.

The invention discloses a rehmannia neutral homogeneous polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine preparations. The polysaccharide mainly comprises galactose, glucose and fructose, the molar ratio of the galactose to the glucose to the fructose is 2.72: 1.17: 1.11, and the weight-average molecular weight of the polysaccharide is 2152 Da. The rehmannia neutral polysaccharide can play a role in treating osteoporosis by increasing bone mineralization amount and bone mineral density; the proliferation and directional osteogenic differentiation of the bone marrow mesenchymal stem cells BMSCs can be promoted, and the obvious application effect is achieved in the aspect of preparing the bone defect treatment medicine through the stem cells. The rehmannia neutral homogeneous polysaccharide shows a huge application prospect in the aspects of preparation of medicines for treating osteoporosis and stem cell treatment (especially bone regeneration treatment based on mesenchymal stem cells). A new candidate substance and a research direction are provided for developing an efficient and safe bone metabolism disease treatment strategy derived from traditional Chinese medicines.

The invention discloses sialylated-N-acetyl lactosamine and synthesis thereof, sialic acid (Neu5Ac) and N-acetyl lactosamine (LacNAc) or sialic acid (Neu5Ac), galactose (Gal), N-acetyl glucosamine (GlcNAc) and the like are used as raw materials, and the sialylated-N-acetyl lactosamine is synthesized through a one-step method. The method for catalytically synthesizing 6 '-SLN and 3'-SLN through a novel one-pot multi-enzyme catalytic system containing an NTP cyclic regeneration system is developed, the method is low in production cost and high in synthesis efficiency, and a foundation is laid for industrialization of sialic acid oligosaccharides such as 6 '-SLN and 3'-SLN.

The invention relates to a method for establishing a blood-brain barrier organ aging model and an intervention method for the model, and the method comprises the following steps: S1, model preparation: preparing cryopreserved brain microvascular endothelial cells, resuscitating the cryopreserved brain microvascular endothelial cells, preparing a culture bottle containing a culture medium for later use, preparing a culture chip and an inducer, and treating the culture chip and the inducer for later use; s2, cell culture: taking the recovered brain microvascular endothelial cells in S1, inoculating the brain microvascular endothelial cells in a culture bottle, culturing and carrying out cell passage to obtain brain microvascular endothelial cells in a logarithmic phase; s3, constructing a blood-brain barrier, inoculating the brain microvascular endothelial cells in the logarithmic phase obtained in the S2 to a chip, and culturing to obtain a blood-brain barrier model; and S4, constructing an aging model, taking the blood-brain barrier model in S3, and performing injury treatment to obtain the blood-brain barrier organ aging model. A D-galactose drug group is used for successfully inducing the aging injury of the blood-brain barrier organ model, and a reliable in-vitro blood-brain barrier organ aging injury model is provided.

The present application relates to the field of bioengineering and microbial technology, and relates to a yeast surface display construction method of a double-enzyme system. AGA1 The gene promoter is replaced by a strong constitutive promoter TEF1 and a glucose-responsive promoter HXT7 ; a double-enzyme free expression vector containing a galactose-inducible promoter GAL1 driven detoxification enzyme gene is constructed, and is transformed into a modified chassis cell to obtain a double-enzyme co-display engineering bacterium; the chassis protein AGA1 is expressed in a culture medium, and YtdepA and YtdepB are induced in a galactose-induced culture medium, so that the YtdepA and YtdepB are co-displayed on the yeast surface through the coupling mechanism of AGA1-AGA2. The strong constitutive promoter TEF1 and the glucose-repressed promoter HXT7 are coupled with the galactose-inducible promoter GAL1 respectively, so that the chassis protein and the detoxification enzyme are matched in the expression timing and intensity, and the detoxification enzyme is displayed on the yeast surface.

The invention provides a composition for improving learning memory, spatial memory and / or cognitive functions and application thereof. The composition disclosed by the invention comprises DHA (docosahexaenoic acid) and galactose, and the mass ratio of the DHA to the galactose is 1: (8-260). The invention also provides a food containing the composition, and an application of the composition or the food in preparation of a product helpful for improving spatial memory, working memory and / or cognitive function.

This invention discloses a sulfated arabinoglycoside, its preparation method, and its applications, belonging to the field of biomedical technology. The technical solution includes a sulfated arabinoglycoside derived from *Streptococcus linearis* polysaccharide; a degree of polymerization of 2-5; arabinose as the main monosaccharide, and containing galactose; the molar percentage of arabinose is not less than 80%, and the molar percentage of galactose is not more than 20%; the main chain is composed of (1→4)-β-L-pyranose arabinose groups, with the sulfate groups of the arabinose mainly substituted at the C-3 position. This invention is applied to inhibiting the abnormal aggregation of human pancreatic islet amyloid peptide (hIAPP), solving the problem of the lack of safe and effective intervention methods for the abnormal aggregation of human pancreatic islet amyloid peptide in existing technologies. It features stable raw material sources, high safety, a simple and mild preparation method suitable for large-scale preparation, and the ability to inhibit the abnormal aggregation of human pancreatic islet amyloid peptide and reduce pancreatic β-cell damage.

The invention discloses an additive formula capable of remarkably reducing the proportion of G0F-N in antibody glycosylation, and the additive formula comprises 2-mercaptoethanol, hydrocortisone, galactose, mannose, 1, 4-diaminobutane dihydrochloride and N-acetylcysteine. The use method comprises the following steps: preparing 1 mM mother liquor from 2-mercaptoethanol by using original liquid, preparing 1 mM mother liquor from hydrocortisone by using absolute ethyl alcohol, preparing 200 g / L mother liquor from galactose by using ultrapure water, preparing 100 g / L mother liquor from mannose by using ultrapure water, preparing 1 g / L mother liquor from 1, 4-diaminobutane dihydrochloride by using ultrapure water, and preparing 1 g / L mother liquor from N-acetylcysteine by using ultrapure water. Wherein 2-mercaptoethanol and hydrocortisone are used as an additive group 1 for combined use, galactose and mannose are used as an additive group 2 for combined use, and 1, 4-diaminobutane dihydrochloride and N-acetylcysteine are used as an additive group 3 for combined use. In the cell culture process, the additives are combined according to different concentrations to achieve the effect of remarkably reducing G0F-N in antibody glycosylation.

The invention relates to the technical field of food processing, in particular to a functional oligosaccharide-containing glycolipid double-control composite tablet candy, which is prepared from the following raw materials: functional oligosaccharide, a lipid control component, a sweet base, microcrystalline cellulose, magnesium stearate, monoglyceride and beta cyclodextrin, the functional oligosaccharide is selected from one or more of fructo-oligosaccharide, isomaltooligosaccharide and galactooligosaccharide; the fat control component is selected from one or more of phytosterol ester and conjugated linoleic acid glyceride; the sweet base material comprises maltitol and isomaltulose; the raw materials can also comprise a mulberry leaf extract. According to the invention, a compound system of the functional oligosaccharide, the special fat control component and the auxiliary sugar control component is adopted, and all the components form a synergistic effect, so that not only can the glycemic index be effectively reduced, but also the fat absorption can be obviously inhibited, the double control of the sugar and the fat in a real sense is realized, and compared with the existing single-function product, the efficacy is more comprehensive and more obvious.