546 results about "Galactose" patented technology

The invention discloses application of burdock root polysaccharide in preparation of a medicine for treating cognitive impairment, and belongs to the technical field of traditional Chinese medicines. Experiments prove that the burdock root polysaccharide can improve cognitive impairment and autonomous exploration ability of D-galactose induced cognitive impairment model mice, increase the activity of superoxide dismutase in serum of the mice, reduce the content of malondialdehyde, reduce the content of hippocampus interleukin-1beta and tumor necrosis factor-alpha in brains of the mice, and improve the cognitive impairment activity of the mice. According to the present invention, with the composition, the loss of the neuron in the hippocampal region of the cognitive impairment model mouse can be reduced so as to achieve the neuron protection effect, such that the good treatment effect on the cognitive impairment can be provided, and the new approach is provided for the development of the cognitive impairment-related drug.

The invention discloses a preparation method of an oral liver protection composition containing ergothioneine, and relates to the technical field of ergothioneine application. The oral liver protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.5 to 5 parts of the ergothioneine, 10 to 30 parts of silibinin, 20 to 50 parts of a phospholipid complex, 5 to 15 parts of a liver targeting carrier, 2 to 8 parts of a disintegrating agent, 1 to 5 parts of an adhesive and 2 to 4 parts of an antioxidant. Free radicals are efficiently captured through a polycyclic conjugated pi electron system of the antioxidant, a hydrogen bond network is formed by a heteroatom bridging effect of the antioxidant, a galactose group of the liver targeting carrier and the disintegrating agent, and the stability of the system is synergistically improved; the phospholipid complex is embedded with a hydrophobic drug and a hydrophilic carrier, and the drug is accurately delivered to the liver by combining the specific recognition capability of the liver targeting carrier; the ergothioneine and the silybin form a bidirectional synergistic liver protection mechanism by removing reactive oxygen free radicals and regulating and controlling cell signal channels respectively.

The invention relates to the technical field of natural compounds, in particular to ganoderma leukemia polysaccharide and a preparation method thereof. The ganoderma leukemia polysaccharide comprises galactose, glucose and mannose, and the molar ratio of the galactose to the mannose to the glucose is (1.5-3.5): (15-30): (65-85); the ganoderma leukemia polysaccharide comprises:-> 6)-Glcp-(1-> dominant residues; (-> 6)-Manp-(1->,-> 4)-Glcp-(1-> residue; and-> 4, 6)-Manp-(1->,-> 3, 6)-Galp-(1->. The ganoderma leukemia polysaccharide has anti-oxidation and detoxification capabilities, can avoid neurodegenerative diseases, reduce lipid accumulation and prolong the service life, and also has high-temperature resistance.

The invention provides a milk powder composition containing breast milk oligosaccharide and easy to digest and absorb. The milk powder composition comprises the breast milk oligosaccharide, OPN, galactooligosaccharide, lactoferrin, alpha-whey protein, multivitamins, lactose, vegetable oil and compound minerals, wherein the weight ratio of the lactoferrin to the OPN to the breast milk oligosaccharide to the galactooligosaccharide is 1: (2-4): (2-50): (4-100). The composition can effectively recover and improve the intestinal barrier function caused by inflammatory factors, can be well absorbed by infants with poor digestion and absorption functions, reduces diarrhea and promotes skeletal development.

The invention relates to an extraction method and application of a sarcodia montagneana extract for inhibiting bacteria, removing dandruff, controlling oil and relieving, and belongs to the technical field of sarcodia montagneana extracts. The extraction method of the sarcodia montagneana extract comprises the following steps: cleaning fresh sarcodia montagneana with deionized water, soaking the cleaned sarcodia montagneana in a compound solution, grinding the soaked sarcodia montagneana to obtain slurry, freeze-drying the slurry, putting the freeze-dried slurry into a supercritical fluid extractor, adjusting corresponding temperature and pressure, and then extracting the freeze-dried sarcodia montagneana by using a supercritical fluid extractor; and fully contacting the supercritical fluid with the sarcodia montagneana powder, performing extraction treatment, and adjusting pressure through a pressure reducer for separation to obtain the sarcodia montagneana extract. The sarcodia montagneana extract contains galactose, fucose, mannose and glucuronic acid, has a remarkable removal effect on ROS and DPPH free radicals, has high antioxidant activity, can serve as a natural antioxidant and has good effects of inhibiting bacteria, removing dandruff, controlling oil and relieving.

The invention provides a method for synthesizing D-psicose through fermentation of escherichia coli by using D-glucose and D-xylose. The method comprises the following steps: by taking escherichia coli as a host cell, over-expressing a galactose permease gene galP, a glucokinase gene glk, a 6-phosphate glucose isomerase gene pgi, a D-psicose-6-phosphate epimerase gene alsE with an SUMO tag and a D-psicose-6-phosphate phosphatase gene a6PP, and constructing a path for synthesizing D-psicose by taking D-glucose and D-xylose as substrates; the synthetic route of D-psicose is optimized by knocking out a D-glucose phosphorylation transport pathway gene ptsG, knocking out D-fructose-6-phosphokinase genes pfkA and pfkB, silencing a glucose-6-phosphate dehydrogenase gene zwf, overexpressing glutamate transhydrogenase genes gdh1 and gdh2, knocking out a UDP-glucose-4-epimerase gene galE and knocking out a transport protein key gene fryA in D-psicose; and finally, the optimal proportion of mixed sugar fermentation is explored, so that efficient synthesis of D-psicose from escherichia coli through mixed sugar fermentation of D-glucose and D-xylose is realized.

The invention provides a method for separating and purifying galactose and glucose by using a simulated moving bed chromatography system, and relates to the technical field of saccharide separation and purification. According to the method, the simulated moving bed chromatography system is used for separating and purifying the galactose, operation can be carried out under the set temperature condition, the separation and purification efficiency of the galactose is remarkably improved, continuous large-scale efficient separation and purification of the galactose can be achieved, the separation and purification effect is good, the yield is high, and the product quality is good. According to the method, pure water is used as an eluent, and analyzed and purified compounds are free of damage, low in toxicity, rich in source and low in cost.

The invention relates to a saccharomyces cerevisiae galactose inducible promoter, an expression vector and application thereof, and belongs to the technical field of biology. The invention provides a method for improving the expression intensity of a galactose inducible promoter in saccharomyces cerevisiae, 1-7 upstream activation sequences are inserted into the galactose inducible promoter PGAL1, and the related upstream activation sequences are derived from different types of yeast galactose inducible promoters. Galactose inducible unidirectional and bidirectional promoter libraries and expression vectors are respectively constructed on the basis, the optimal unidirectional inducible promoter UIP-A2 is obtained through screening, the strength of the optimal unidirectional inducible promoter UIP-A2 is 2.37 times that of PGAL1, and the induction strength of the optimal bidirectional inducible promoter in two directions is 6.02 times and 3.87 times that of the optimal bidirectional inducible promoter before modification respectively; by using the method provided by the invention, the expression intensity of the galactose inducible promoter in the saccharomyces cerevisiae can be remarkably improved, a powerful tool is provided for efficiently expressing exogenous genes by the saccharomyces cerevisiae, and the method has a wide application prospect.

The invention discloses saccharomyces cerevisiae recombinant bacteria for efficiently producing terpenoids as well as a construction method and application of the saccharomyces cerevisiae recombinant bacteria. According to the construction method, saccharomyces cerevisiae rich in terpenoid precursor farnesyl pyrophosphate is taken as a starting strain, the GAL80 protein level in a galactose induction system is regulated and controlled by introducing an N-Degron strategy, and meanwhile, a glucose concentration response type promoter HXT1 is introduced to effectively regulate and control the expression of GAL80 from the transcriptional level; the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids can be obtained. The strategy is applied to a saccharomyces cerevisiae engineering strain of beta-elemene and patchouli alcohol, and a remarkable effect is achieved. And in the high-density fermentation process of the saccharomyces cerevisiae, the yield of the beta-elemene is obviously increased and reaches 4818.6 mg / L. The invention provides a solid research basis for sustainable biosynthesis of terpenoids, and has a wide application prospect.

The invention discloses allium fistulosum and bolete polysaccharide as well as a preparation method and application thereof. The allium fistulosum and bolete polysaccharide is a glycopolymer composed of arabinose, xylose, mannose, glucose and galactose. The allium fistulosum bolete polysaccharide disclosed by the invention has strong antioxidant capacity, has a stimulation and activation effect on macrophage immune response, has an inhibition effect on alpha-glucosidase, has the effects of resisting oxidation, enhancing immunity and reducing blood sugar, and can be applied to development of health-care foods and functional foods.

The present invention belongs to the field of enzyme engineering and food engineering technology, and specifically relates to the directed evolution and site-directed mutagenesis of a lactase enzyme molecule and its application in fresh milk. The amino acid sequence of the lactase mutant obtained by the present invention is shown in SEQ ID NO.4. Through the directed artificial evolution of the lactase molecule, a new lactase molecule is obtained that can efficiently convert lactose in fresh milk and synthesize oligosaccharides at a relatively low temperature, and is rapidly inactivated at a subsequent elevated temperature (such as pasteurization). This facilitates the highly nutritious in situ bioconversion of lactose in fresh milk into prebiotic oligosaccharides, further improving the nutritional value of fresh milk and expanding the fresh milk consumer group.

The invention discloses an application of cordyceps sobolifera polysaccharide in preparation of a medicine for intervening in male dyszoospermia and reproductive injury, the molecular weight of the cordyceps sobolifera polysaccharide is 2.92 * 10 < 4 > Da, the cordyceps sobolifera polysaccharide is composed of galactose, mannose and glucose according to the molar ratio of 68.125: 43.875: 12, a novel cordyceps sobolifera homogeneous polysaccharide component CCP-1 is separated and purified, and the cordyceps sobolifera polysaccharide can be used for preparing a medicine for intervening in male dyszoospermia and reproductive injury. The influence on reproductive injury of a cyclophosphamide-induced immunocompromised male mouse and a potential mechanism thereof are discussed through an HPT axis; an apoptosis inducer is used for inducing mouse spermatogont-GC-1spg cells to construct an apoptosis model to discuss the influence on cell apoptosis; the understanding of the cordyceps sobolifera polysaccharide CCP-1 as a potential health supplement for intervening male dyszoospermia and reproductive injury is improved.

Children's formula milk containing added human milk oligosaccharides, and a preparation method therefor. Raw materials of the children's formula milk include cow's milk, phospholipids, compound nutrients, whey protein powder, prebiotics, and / or condensed milk; and the prebiotics comprise human milk oligosaccharides, galacto-oligosaccharides, and fructo-oligosaccharides. The children's formula milk provides main nutritional requirements for children, and also has the effects of enhancing immunity and regulating gut microbiota.

The invention discloses a preparation method of soybean milk powder for promoting immune regulation, and belongs to the technical field of soybean product processing. Performing biological enzymolysis; modifying soybean protein; compounding functional components; and preparing the microcapsule. An ultrasonic-enzyme composite extraction technology is applied, and the enrichment rate of components with immunocompetence is increased. According to the method, the Maillard reaction is performed on the soybean protein and the polysaccharide by utilizing an ultrasonic-assisted heating technology, so that the emulsifying characteristic of the soybean protein isolate is improved, and the immunoregulation promoting performance of the soybean protein isolate is greatly improved. According to the invention, through a microcapsule embedding technology, glycosylated soybean protein is wrapped in the particles by a wall material formed by galactooligosaccharide and resistant dextrin, the stability and bioavailability are improved, the microencapsulation efficiency is greater than 95%, and the immunoregulation promotion performance of the microencapsulated soybean protein is effectively exerted. By adopting spray drying, inactivation of immune active components caused by high temperature is avoided.

The invention provides a whitening composition, whitening essence and a preparation method thereof, the whitening composition is prepared from the following raw materials in parts by weight: 10 to 15 parts of extract compound, 3 to 5 parts of compound polysaccharide, 2 to 4 parts of galactose yeast-like bacterium fermentation product filtrate, 1 to 3 parts of rhodopseudomonas palustris liquid, 0.1 to 0.3 part of soluble collagen and 0.5 to 1 part of superoxide dismutase; the extract compound is prepared from the following raw materials in parts by weight: 5 to 7 parts of radix cynanchi atrati extract, 3 to 5 parts of vitex agnus linteus extract, 1 to 3 parts of mussel extract, 2 to 4 parts of wild soybean seed extract and 1 to 3 parts of chamomilla recutita flower extract. The components of the whitening composition are reasonably compounded, the whitening composition is mild and non-irritant, synergistic interaction is achieved, rapid first-aid whitening can be achieved, 15-min first-aid whitening can be achieved, the skin can be restored to be bright and white, and the freckle removing and whitening effects can be comprehensively achieved by damaging melanocytes, inhibiting melanin generation and promoting melanin degradation. Meanwhile, the repairing, moisturizing and soothing effects are considered, no irritation is caused, and the safety is high.

The invention is applicable to the technical field of biology, and provides a method for rapidly extracting plant rhizome extracellular vesicles from monosaccharide and compound enzyme. According to the method, the plant extracellular vesicles are extracted by an enzymolysis-monosaccharide solution extraction coupling method for the first time, and the defect that an extraction method is single is overcome. According to the method, the conditions are mild, the primary property, the integrity and the biological activity of the vesicle structure are kept, the extraction purity is high, and the particle size distribution is uniform and concentrated in the range of 20-300 nm. The total protein extraction amounts of glucose, galactose and fructose solutions are respectively increased by 36.09% + / -1.18%, 37.65% + / -2.83% and 36.83% + / -2.59% compared with those of PBS (Phosphate Buffer Solution) extraction treatment. In addition, the'single ultracentrifugation 'purification process overcomes the problem of time consumption of traditional gradient centrifugation, and the time is shortened by more than 3h compared with that of a traditional extraction method. The method breaks through the technical bottleneck of large-scale production of the plant extracellular vesicles, and has great application and popularization values.

The present invention relates to a D-galactopyranose compound of formula (1)whereinthe pyranose ring is α-D-galactopyranose, and these compounds are high affinity galectin-1 and / or galectin 3 inhibitors for use in treatment of inflammation; fibrosis; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer; metastasising cancers; autoimmune diseases, metabolic disorders; heart disease; heart failure; pathological angiogenesis; eye diseases; atherosclerosis; metabolic diseases; diabetes type I; diabetes type II; insulin resistance; Diastolic heart failure; asthma; liver disorders.

The invention discloses an analysis method for determining the content of galactosamine in sulfated polysaccharide based on high-temperature hydrochloric acid hydrolysis and an ion chromatograph method, which comprises the following steps: hydrolyzing a test sample with hydrochloric acid at high temperature to release galactosamine, separating with an ion chromatograph and a sugar analysis column, detecting with a pulsed ampere detector, and determining the content of galactosamine in sulfated polysaccharide according to a galactosamine reference substance. And quantitatively analyzing the content of galactosamine in the test sample. The method disclosed by the invention has the advantages of simplicity, rapidness, accuracy, high efficiency, no need of derivation treatment and the like.

The invention belongs to the technical field of gene engineering, and particularly relates to a genetically engineered bacterium of galactosylceramide as well as a construction method and application of the genetically engineered bacterium of galactosylceramide. On the basis of an existing delta pyrG aspergillus oryzae auxotroph strain, the metabolic flux of neutral glycosphingolipid is enhanced through overexpression of an AoCerS gene; meanwhile, the expression of a neutral sphingolipid glucosylceramide (GlcCers) synthetase gene AoUGCG is inhibited, so that the common intermediate metabolite flux of the GlcCers and galactosylceramide (GalCers) flows to the synthesis of the GalCers. The GalCers relative content of the constructed aspergillus oryzae engineering strain is increased by more than two times compared with that of a control strain, and is increased by about 70% compared with that of the control strain.

The invention relates to an antibody drug conjugate as well as a preparation method and application thereof, the preparation method of the antibody drug conjugate comprises the following steps: S10, obtaining a wild type antibody, and carrying out hydrolysis reaction on galactose at the tail end of a sugar chain of the wild type antibody to obtain an antibody of which the tail end is four GlcNAc sugar types; s20, obtaining B4GALT1 (Y286L), mixing the UDP-GalNAz, the B4GALT1 (Y286L) and the antibody, and carrying out an enzymatic reaction so as to transfer the azido galactose to the tail end of a carbohydrate chain of the antibody, so as to obtain a carbohydrate chain modified antibody; and S30, mixing a target drug with the carbohydrate chain modified antibody, and carrying out a one-step click chemical reaction to obtain the antibody drug conjugate. The galactose hydrolase can retain four GlcNAc sites, and after the galactose hydrolase is connected with GalNAz, four target drug molecules can be combined, the DAR value of the antibody drug conjugate is increased, and the drug activity of the antibody drug conjugate is improved.

The invention provides a bifidobacterium animalis subspecies HX-BA21, and the preservation number of the bifidobacterium animalis subspecies HX-BA21 is CGMCC (China General Microbiological Culture Collection Center) NO.32958. The invention discloses bifidobacterium animalis subspecies HX-BA21 containing exosome metabiotics and an exosome-containing fermentation compound obtained by fermenting gastrodia elata through the HX-BA21. The fermentation compound comprises a probiotic protective layer (a core layer), an exosome delivery layer (a shell layer) and a prebiotics sustained release layer (a cocoon layer) from inside to outside. The probiotic protection layer comprises a sodium alginate-milk fat globule membrane compound and animal bifidobacterium subsp.animalis; the exosome delivery layer comprises a gelatin-chitosan compound and a gastrodia elata and probiotic mixed exosome; the prebiotic sustained-release layer comprises an octenyl succinic anhydride starch / polyvinyl alcohol compound and inulin. The compound can start cell repair by improving the cell migration rate, up-regulate DNA helicase and telomere related gene expression, delay premature senescence and delay cell senescence caused by D-galactose by adjusting the oxidative stress level.

The present invention provides methods for glycosylation and compound preparation. The compounds include galactosylated, sialylated, fucosylated, and N-acetylglucosaminated compounds from simple oligosaccharides and sugars of animal, plant, or microbial origin. In certain embodiments, the invention provides for trinucleotide-free enzymatic production of oligosaccharides starting from simple sugars, including plant-based sugars. The present invention also provides methods of enzymatic production of fucosylated oligosaccharides and fucosylated antibody-glycan conjugates from ordinary enzymes. The production may be a cell-free, one-pot synthesis using an enzyme and, in some embodiments, an immobilized enzyme. The synthesis is a highly customizable and efficient cell-free manufacturing process. In some embodiments, lactose derivatives and human milk oligosaccharides (HMOs) are produced.

The invention provides coconut oil essence hand cream and a preparation method thereof. The hand cream is prepared from the following raw materials in parts by weight: 18 to 24 parts of coconut oil essence, 12 to 15 parts of compound tremella extract, 3 to 6 parts of fig fruit extract, 7 to 10 parts of grape seed oil, 6 to 9 parts of olive fruit oil, 4 to 7 parts of squalane, 5 to 8 parts of fucoidin, 3 to 6 parts of galactooligosaccharide, 2 to 4 parts of dipotassium glycyrrhizinate, 10 to 14 parts of propylene glycol, 12 to 15 parts of glycerol, 0.01 to 0.05 part of preservative, 10 to 14 parts of sucrose fatty acid ester, 13 to 17 parts of sorbitan olivate, 0.2 to 0.6 part of essence and 140 to 180 parts of water. Through the mutual synergistic effect of the raw materials such as the coconut oil essence, the composite tremella extract, the fig extract, the grape seed oil, the olive fruit oil, the squalane, the fucoidin and the galactooligosaccharide, moisture of hand skin can be effectively locked, a protective layer is formed on the surface layer of the skin, loss of moisture of the hand skin is reduced, and the hand cream has a good hand moisturizing effect. Therefore, the hand skin is kept in a moisturized state, and the problems of skin dryness, desquamation, cracking and the like are effectively reduced.

The invention relates to a beta-galactosidase mutant with improved thermal stability and pH stability, and belongs to the technical field of enzyme engineering. The beta-galactosidase derived from Kluyveromyces lactis is subjected to molecular modification, a potential mutation site is determined by using an alanine scanning strategy, beta-galactosidase mutants Pro433Ala and Asp441Ala of which the thermal stability and the pH stability are simultaneously improved are obtained through site-directed mutagenesis screening, the catalytic efficiency of the mutants is improved to 1.03-1.18 times compared with that of the mutants before optimization, and the beta-galactosidase mutant can be used for preparing beta-galactosidase. Wherein the maximum half-life period of the mutant Asp441Ala under the condition of 50 DEG C reaches 91.5 minutes and is increased by 213.4% compared with that of a wild type, the problems of poor catalytic thermal stability and poor pH stability of beta-galactosidase in the prior art are solved, and a brand-new tool is provided for obtaining galactooligosaccharide by using beta-galactosidase to hydrolyze lactose.

The invention relates to the technical field of functional food preparation, and discloses solid soft sweets capable of remarkably relieving asthenopia and a preparation method of the solid soft sweets, and every 100 parts by mass of the soft sweets comprise the following components: 5.8-6.2 parts of lutein ester microcapsules, the wall material of which is starch octenyl succinate with the substitution degree of 0.02-0.04; the emulsifier is lecithin of which the purity is greater than or equal to 90%, and the particle size lt of the emulsifier is 1t; the particle size is 200 nm; 75 to 85 parts of blueberry anthocyanin-VC compound freeze-dried powder; 18 to 22 parts of lycium barbarum polysaccharide; 45 to 55 parts of galactooligosaccharide; 8 to 12 parts of medium chain triglyceride; and a colloid base material. Through collaborative design of a hydrophobic modified wall material and a gradient mixing process, a multi-level protection network of active ingredients is constructed, so that the retention rate of fat-soluble nutrients in the processing and storage period is increased to 90% or above.

The invention discloses a substance composition. The present invention relates to a conjugate comprising a deglycosylated IgA-binding moiety, a cell receptor-binding moiety that binds to a hepatocyte or other degraded cell of a patient or subject through an asialoglycoprotein receptor (ASGPR) on the surface of the hepatocyte or other degraded cell, and optionally a linker moiety that links the deglycosylated IgA-binding moiety and the cell receptor-binding moiety, wherein the composition of matter can be used to remove galactose deficient IgA1 in a patient or subject.

The invention provides polygonatum kingianum polysaccharide and application thereof in a medicine for treating diabetes mellitus, the molecular weight of the polygonatum kingianum polysaccharide is 2365Da, and the polygonatum kingianum polysaccharide is uniform polysaccharide; the polygonatum kingianum polysaccharide is prepared from arabinose, fructose, galactose, glucose and ribose according to the mass ratio of 39.19: 24.28: 2.85: 1.37: 3.38, the polygonatum kingianum polysaccharide is simple in preparation method, low in cost and free of pollution, and the obtained polysaccharide is high in purity; an in-vitro lipid-lowering and blood-glucose-lowering activity experiment result of the polygonatum kingianum polysaccharide shows that the polygonatum kingianum polysaccharide can obviously reduce lipid accumulation of human liver cancer HepG2 cells and obviously improve glucose consumption of insulin-resistant cells, the polygonatum kingianum polysaccharide has good in-vitro lipid-lowering and blood-glucose-lowering activity, and a new way is provided for treating and preventing diabetes mellitus.

The invention relates to the technical field of pharmaceutical compositions, and particularly discloses a proteolipid composite supplement with synergistic hangover alleviating and liver protecting functions and a preparation method thereof. The health-care food comprises the following components in percentage by mass: 10-25% of a Pu'er tea extract, 15-30% of glucose syrup, 5-15% of a hovenia acerba extract, 5-15% of a radix puerariae extract, 8-20% of corn peptide, 5-15% of oyster peptide, 3-10% of earthworm peptide, 1-5% of malic acid, 2-8% of multivitamins, 1-5% of an electrolyte compound, 3-10% of prebiotic fibers and 0.5-3% of gamma-aminobutyric acid. The compound vitamins comprise B1, B2, B6, B12, folic acid and vitamin C; the electrolyte compound is a nanoscale electrolyte compound and is an organic chelate containing potassium, magnesium and zinc; the prebiotic fiber is prepared by compounding galactooligosaccharide and resistant dextrin; through compounding design of active peptide components, plant active ingredients and functional factors, the active peptide improves the activity of key enzymes in ethanol metabolism in a targeted manner, and alcohol decomposition is accelerated.

The invention relates to the technical field of dairy product processing, in particular to high-protein native-dietary-fiber-rich zero-lactose milk and a preparation method thereof.The method comprises the following steps that 1, raw milk is treated through a reverse osmosis membrane, and RO penetrating fluid and RO retention fluid are obtained; 2) adding a proper amount of No.1 lactase into the RO retention solution, and hydrolyzing until the lactose concentration is less than or equal to 2g / 100mL and the galactooligosaccharide concentration is more than or equal to 3g / 100mL; 3) pasteurizing the hydrolyzed product, and inactivating the No.1 lactase to obtain a semi-finished product milk liquid containing the native dietary fibers; and 4) adding a proper amount of No.2 lactase into the semi-finished product milk liquid, and hydrolyzing until the lactose concentration is less than 0.5 g / 100mL to obtain the high-protein raw milk rich in native dietary fiber and zero in lactose. According to the method, lactase is subjected to enzymolysis on the premise that the sweetness of milk is not improved, galactooligosaccharide (dietary fiber) is generated in a natural enzymolysis mode, and the natural nutritional value of the product is improved.

The invention discloses zero-lactose milk containing galactooligosaccharide and a preparation method of the zero-lactose milk, and belongs to the technical field of dairy products. By means of the synergistic effect of the immobilized lactase and the acerola cherry powder extract, efficient hydrolysis of lactose is achieved, the lactose content is reduced to 0.1-0.5 g / 100 mL, meanwhile, 1.0-2.5 g / 100 mL of galactooligosaccharide is generated, and the lactase is especially suitable for people with lactose intolerance to drink. The preparation method comprises the following steps: pasteurizing the raw milk at 60-65 DEG C for 20-40 minutes, and then cooling; adding 0.01 to 0.3 percent of immobilized lactase and 0.001 to 0.1 percent of acerola cherry powder extract under the condition of 20 to 50 DEG C, and stirring and reacting for 10 to 60 minutes; then heating at the temperature of 80-120 DEG C for 5-20 minutes for enzyme deactivation; performing ultrasonic homogenization (200 to 500W, 20 to 70kHz, 10min), cooling and filling; and finally, carrying out ultrahigh pressure sterilization for 5 minutes at 500 MPa and 28 DEG C to ensure that the product is sterile. The stability and the repeated utilization rate of the enzyme are improved through the immobilized enzyme technology, and due to the addition of the acerola cherry powder extract, the product is endowed with natural oxidation resistance while the lactose is synergistically hydrolyzed.