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Method for producing wood sugar product

A production method and technology for xylose, applied in the production of sugar, sugar production, microorganism-based methods, etc., can solve the problems of inability to crystallize, affecting xylose crystallization, accelerating the aging and death of yeast, etc.

Active Publication Date: 2010-03-03
SHENGQUAN HEALTANG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patented technology also has the following two defects: (1) xylose hydrolyzate or xylose mother liquor are directly added to Saccharomyces cerevisiae without pretreatment so that fermentation is not easy to carry out. Toxic substances, such as: pigment substances with benzene rings, polymer compounds, heavy metal ions, chlorides, etc.
These poisons will accelerate the aging and death of yeast, so that the fermentation time is long and incomplete
(2) Glucose is only removed, and galactose is also contained in the sugar solution. The existence of galactose affects the crystallization of xylose, thereby reducing the crystallization yield or even failing to crystallize
[0005] In summary, in the prior art, there is no production method with low cost and high efficiency that can realize large-scale industrial production of high-purity L-arabinose and xylose / xylitol

Method used

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Examples

Experimental program
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Effect test

example 1

[0227] Example 1, The specific process parameters for the xylose fraction to use Saccharomyces cerevisiae to ferment glucose and galactose to produce ethanol under anaerobic conditions are as follows: the percentages are mass percentages.

[0228] (1) first adjust the sugar concentration of the xylose syrup to 10-40%, preferably 20-35%;

[0229] (2) The composition of the strain culture medium is: urea or ammonium sulfate: 0.01~0.5%, preferably 0.05~0.2%; potassium dihydrogen phosphate: 0.01~0.5%, preferably 0.01~0.2%; magnesium sulfate: 0.01~0.4% , preferably 0.01 to 0.2%;

[0230] (3) The parameters of the fermentation process are: pH: 2.5~5, temperature 33~45°C, no air or a small amount of air (0.1~0.3vvm) at the beginning of fermentation, when the cell concentration reaches 10 8 per ml, stop the ventilation, and carry out anaerobic fermentation, and the fermentation time is 8 to 22 hours;

[0231] (4) Conversion results: glucose removal rate 97.15-98.5%, galactose remo...

example 2

[0232] Example 2, The xylose fraction uses Saccharomyces cerevisiae to ferment under aerobic conditions to remove glucose and galactose to generate ethanol, carbon dioxide and water. The specific process parameters are as follows: the percentages are mass percentages.

[0233] (1) first adjust the sugar concentration of the xylose liquid to 8-30%, preferably 10-25%;

[0234] (2) The composition of the strain medium is: ammonium sulfate: 0.1~3.5%, preferably 0.5~2.5%; potassium dihydrogen phosphate: 0.1~5%, preferably 0.5-3%; magnesium sulfate: 0.05~4%, preferably 0.2-2%; corn steep liquor (calculated as dry matter): 0.5-20%, preferably 1-10%;

[0235] (3) The parameters of the fermentation process are: temperature 25-45°C, preferably 30-40; pH: 3.5-5.5, air volume 0.2-0.5vvm, fermentation time 10-28h;

[0236] (4) Conversion results: the removal rate of glucose is 95%-98%, the removal rate of galactose is 50%-70%, the yield of ethanol is 90-95%, and the yield of xylose is 96-...

example 3

[0237] Example 3, The xylose fraction uses shikimic acid-deficient Bacillus subtilis to ferment under aerobic conditions to remove glucose and galactose to generate D-ribose. The specific process parameters are as follows: the percentages are mass percentages.

[0238] (1) Medium and culture conditions:

[0239] a. Incline medium: glucose 0.5-2%, preferably 0.8-1.6%; peptone 0.4-2%, preferably 0.8-1.5%; yeast extract 0.1-1%, preferably 0.2-0.6%; sodium chloride 0.1-1.2% , preferably 0.2-0.8%; agar 0.8-3%, preferably 1.5-2.5%; pH 6.0-8.0, preferably 6.8-7.5; culture temperature 30-40°C, preferably 32-38°C; culture time 12-36h, preferably 18~24h.

[0240] B, seed culture medium: glucose 1~3%, preferably 1.5~2.2%; Corn steep liquor (in dry matter) 1~3.5%, preferably 1.5~2.5%; Yeast extract 0.1~1%, preferably 0.2~0.6%; Dipotassium hydrogen phosphate 0.1-1%, preferably 0.2-0.7%; potassium dihydrogen phosphate 0.1-0.8%, preferably 0.1-0.5%; pH 6.0-8.0, preferably 6.8-7.5; cultur...

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Abstract

The invention relates to a method for producing a wood sugar product and a byproduct of ethanol or D-ribose or citric acid. The method takes hydrolysis liquid, wood sugar mother liquor and / or production waste liquid containing pentose of agricultural or forestry waste as the raw materials, comprising the following steps: (a) preprocessing feed liquid; (b) colour spectrum separation: separating thefeed liquid into arabinose fraction feed liquid and wood sugar fraction feed liquid; (c) removing mixed sugar at least containing glucose and galactolipin; and (d) carrying out aftertreatment on thewood sugar fraction feed liquid to obtain the wood sugar product. The wood sugar product comprises xylitol and D-wood sugar. The invention has low production cost and high efficiency and is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the production technology of xylose, especially the production of xylose products by using hemicellulose hydrolyzate, xylose mother liquor and production waste liquid containing five-carbon sugar as raw materials, and simultaneously producing glucose such as ethanol or D-ribose or citric acid Methods of Fermentation Products. The xylose product of the present invention also includes xylitol in addition to D-xylose. technical background [0002] Xylose products such as xylose and xylitol are more and more popular as food sweeteners. Many health care functions of xylose and xylitol have been confirmed by scientific research, such as lowering blood sugar and preventing dental caries. [0003] Plant tissues are mostly composed of cellulose, hemicellulose, lignin and similar substances, among which hemicellulose is mainly composed of L-arabinose and D-xylose. [0004] Chinese patent CN1320122C discloses a method for extracting xy...

Claims

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Application Information

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IPC IPC(8): C07H3/02C07H1/06C07H1/08C07C31/18C07C29/141C07C29/74C13K13/00B01D15/08C12P7/06C12P19/02C12P7/48C12P7/18C12R1/125C12R1/685C12R1/865C12R1/78C12R1/645C12R1/74C12R1/88C12R1/72
CPCY02E50/17Y02E50/10
Inventor 唐一林江成真高绍丰张茜韩文彬马军强
Owner SHENGQUAN HEALTANG
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