45 results about "Shikimic acid" patented technology

The invention discloses a cistanche deserticola extract for improving bone mineral density, the total content of echinacoside and verbascoside in the cistanche deserticola extract is greater than 25wt%, and the cistanche deserticola extract is obtained by adding distilled water into cistanche tubulosa coarse powder for extraction, filtration, filtrate centrifugation, concentration, extraction, concentration and impurity removal, dilution, after-treatment, concentration and drying. The total content of echinacoside and verbascoside in the cistanche deserticola extract for improving the bone mineral density is 25-30 wt%, and the cistanche deserticola extract is rich in shikimic acid, phenylpropionic acid and alkaloid components, so that the cistanche deserticola extract can stably play a role in improving the bone mineral density.

The invention relates to the technical field of preparation of L-tyrosine, and discloses a method for enhancing generation of L-tyrosine in nicotiana benthamiana and application. In order to relieve product negative feedback inhibition of a key enzyme in a production path of L-tyrosine in tobacco and to improve the yield of L-tyrosine in tobacco, DAHPS enzyme derived from microorganisms is subjected to L175Q site mutation and then is connected with a signal peptide AtRs1A; shikimic acid mutase / prebenzoate dehydrogenase TyrAfbr and tyrosine aminotransferase TyrB which are derived from microorganisms are introduced into the tobacco chassis after being connected with the signal peptide Rs1A, the expression has orthogonality, and transient expression is performed in the Bensi tobacco plant chassis by utilizing agrobacterium infection, so that the synthesis capability of L-tyrosine in tobacco chassis cells can be improved; a large amount of L-tyrosine can be directly generated in the tobacco chassis plastid.

The present application relates to the technical field of synthetic biology, in particular to a multi-module synergistic optimization of brown cyanidin and brevifolin production of saccharomyces cerevisiae engineering bacteria and its construction method and application. The saccharomyces cerevisiae engineering bacteria includes naringenin synthesis pathway, optimization pathway of shikimic acid, synergistic pathway of phenylalanine, 6-OH luteolin synthesis pathway, brown cyanidin and brevifolin synthesis pathway, multiple copies of SbF6H and MpalOMT2, NADPH optimization pathway, and SAM cycle optimization pathway. The saccharomyces cerevisiae engineering bacteria is used for producing brown cyanidin and brevifolin, and improving the yield of the two.

The invention discloses a waste lithium ion battery recycling method, and belongs to the technical field of recycling of waste lithium ion batteries. The method comprises the following steps: leaching the positive electrode powder of the waste lithium ion battery in a deep eutectic solvent, and carrying out solid-liquid separation to obtain a leaching solution rich in heavy metals; the eutectic solvent is prepared from shikimic acid, catechol and tetradecyl dimethyl benzyl ammonium chloride. The deep-eutectic solvent is adopted for leaching, the leaching rate of nickel, cobalt, manganese and other heavy metals can be increased, meanwhile, leaching of impurity metals is avoided, and then it is avoided that a large amount of waste water is generated due to impurity removal. The metal ions and the eutectic solvent are separated by using the ion exchange resin, so that the eutectic solvent is recovered and can be repeatedly used, and the pure metal solution is obtained.

This invention relates to the field of traditional Chinese medicine analysis, and particularly to a method for determining the content of multiple components in Terminalia chebula and constructing its characteristic fingerprint spectrum. The method provided by this invention can simultaneously determine chebulin, gallic acid, 4-galloshikimic acid, 5-galloshikimic acid, punicalin (punicalin A&B), corilagin, 1,3,6-tri-O-galloyl-β-D-glucose, chebulin, urolithin M5, chebulin tannic acid, chebulin-lintannic acid, and ellagic acid in Terminalia chebula. It exhibits high sensitivity, good precision, repeatability, and stability, and can be used to detect the tannin content in the extract at each step of the tannin extraction process, as well as for related detection in the study and quality evaluation of tannin components in Terminalia chebula. It can also be used to construct the characteristic fingerprint spectrum of Terminalia chebula. This method can also be used for determining the content of tannin compounds and constructing their characteristic fingerprint spectrum in Terminalia chebula, which contains similar tannin compounds to Terminalia chebula.

The invention discloses a drug-loaded nanoparticle as well as a preparation method and application thereof. The drug-loaded nanoparticle can be used for treating triple negative breast cancer, and specifically comprises a magnetic nanoparticle, an active component loaded in the magnetic nanoparticle, and an erythrocyte membrane coating the magnetic nanoparticle, wherein the active ingredients are selected from one or more of piperlongumine, polyphyllin II, erianin, pseudolaric acid, 6-gingerol, ginkgetin, solasonine, cucurbitacine B and lycorine. In practical application, the drug-loaded nanoparticles have good biological safety, long circulation capability and high tumor retention capability, and can generate efficient killing and treatment effects on triple-negative breast cancer by inducing cell apoptosis.

The application discloses a kind of recombinant escherichia coli for producing L-tyrosine and application, belong to genetic engineering and bioengineering technical field.The application knocks out aroP and tyrP gene, expresses the yddG gene of escherichia coli endogenous to modify the aromatic amino acid transport system of escherichia coli, then heterologous expression comes from bifidobacterium fpk and expresses the ppsA and tktA gene of escherichia coli endogenous to increase the precursor supply of shikimic acid pathway, again through the anti feedback gene expression aroG and tyrA 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase, branch acid mutase and prephenate dehydrogenase are removed feedback inhibition, increase the carbon flux of shikimic acid pathway.Knock out tyrR gene removes the repression of TyrR protein to shikimic acid pathway key enzyme, again knock out trpE and pheA gene to block partial side reaction of shikimic acid pathway, so that the synthesis flux of L-tyrosine increases.Finally knock out endogenous poxB gene to realize the modification of acetic acid pathway, realizes the stable fermentation performance of high glucose concentration.

The invention relates to a composition comprising shikimic acid and an extract of Prunus Mume, and its non-medical and medical use, in particular for use in the treatment of acne.

The application discloses a Pichia pastoris engineering bacterium for converting methanol to synthesize bakuchiol from scratch as well as construction and application of the bacterium. In different chromosomal neutral sites of the Pichia pastoris, a heterologous p-coumaric acid synthesis pathway is introduced, key genes of tyrosine and phenylalanine synthesis pathways of a heterologous shikimic acid pathway are knocked out, overexpressed or heterologously expressed, a high-yield p-coumaric acid strain is obtained, on the basis of the strain, a heterologous bakuchiol biosynthesis pathway is introduced, bakuchiol synthetase is overexpressed, key genes of a MVA pathway and genes of an exogenous acetyl coenzyme A supply pathway are endogenously overexpressed or heterologously expressed, after optimization of a methanol concentration and back complementation of a His4 gene of the strain, the yield of the bakuchiol is effectively increased to 91.2 mg / L, which is 59.8 times higher than that of an initial strain, and the yield of the bakuchiol in a 15L fermenter reaches 692.8 mg / L. The application has the characteristics of high conversion efficiency, low production cost, easy preparation, wide industrial application prospect and the like.

The present invention provides an aromatic compound composition containing at least an aromatic compound (A) having a measured bio-based content of 80% to 100% (inclusive), the measured bio-based content indicating the proportion of radioactive carbon 14C derived from a biomass resource, the measured bio-based content being determined on the basis of the concentration of radioactive carbon in circulating carbon at 1950 as measured in accordance with ASTM D6866-20, the aromatic compound (A) is phenol (A) or 4-hydroxybenzoic acid (A), and when the aromatic compound (A) is phenol (A), the aromatic compound composition is dissolved in pure water so that the concentration of the aromatic compound composition is 90 mass%, the absorbance at a wavelength of 425 nm is 0.001-0.70, and when the aromatic compound (A) is phenol (A), the absorbance at a wavelength of 425 nm is 0.001-0.70. When the aromatic compound (A) is 4-hydroxybenzoic acid (A), the aromatic compound composition has an absorbance of 0.015 or less at a wavelength of 425 nm when the aromatic compound composition is dissolved in ethanol at a concentration of 20 g / L, or when the total amount of the aromatic compound composition is 100 mass%, the absorbance is 0.015 or less at a wavelength of 425 nm. The content of shikimic acid in the aromatic compound composition is 0.012 mass% or less.

The invention relates to a hair care composition comprising malic acid, arginine and shikimic acid, and to the use of said components in the manufacture of a hair care composition.

The present invention relates to a recombinant microorganism, preferably a recombinant yeast, capable of producing caffeic acid comprising a heterologous gene coding for an enzyme of the hydrolase family capable of breaking, preferably of hydrolyzing, the caffeoyl-shikimate bond to produce caffeic acid from caffeoyl-shikimate. Said microorganism, preferably said recombinant yeast, may also be capable of producing ferulic acid from the caffeic acid obtained. The present invention also relates to a method for producing caffeic acid and a method for producing caffeic acid and / or ferulic acid, using microorganisms, preferably yeasts, according to the invention. Finally, the invention also relates to the use of microorganisms, preferably yeasts, according to the invention to produce caffeic acid and / or ferulic acid.

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral / antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral / antibacterial exceeding a 3 log 10 reduction in viral titer.

The invention relates to the technical field of synthetic biology, in particular to a multi-module collaborative optimization high-yield Jaceosidin and Eupatorin Saccharomyces cerevisiae engineering bacterium as well as a construction method and application thereof. The saccharomyces cerevisiae engineering bacteria comprise a synthetic route of naringenin, an optimized route of shikimic acid, a synergistic route of phenylalanine, a synthetic route of 6-OH luteolin, a synthetic route of jaceosidin and eupatorin, multiple copies of SbF6H and MpalOMT2, an optimized route of NADPH (nicotinamide adenine dinucleotide phosphate) and an optimized route of SAM circulation. The saccharomyces cerevisiae engineering bacterium is used for producing jaceosidin and eupatorin, and the yield of jaceosidin and eupatorin is increased.

The present invention pertains to a method for producing an antimicrobial composition preferably comprising piceol. Moreover, the invention relates to an antimicrobial composition comprising or consisting of piceol, preferably in an excess amount compared to picein, further plant-derived substances and optionally picein. The invention further relates to a cosmetic, personal care, household, home care, pet care or pharmaceutical product comprising said antimicrobial composition. Moreover, the invention relates to the use of a microorganism, preferably a lactobacillus species, for producing an antimicrobial composition comprising piceol from a composition, preferably a plant-extract, comprising picein. In addition, the invention relates to the use of shikimic acid for increasing the antimicrobial efficacy of piceol and / or to the use of piceol for increasing the antimicrobial efficacy of shikimic acid. Further embodiments and aspects of the invention are presented in the attached claims as well as in the description and examples below.

The invention discloses a selective reduction method of 5-O-methylsulfonyl ethyl shikimic acid ketal (acetal), which can be used for preparing a Tamiflu key intermediate (Roch epoxy intermediate). A series of ketal (acetal) compounds I-1-10 and a reducing agent Et3SiH are subjected to selective reduction reaction under mild conditions under the action of a catalyst AlCl3 to obtain C-3 allylic position various substituted ether compounds II-1-10, and the yield is 65-97% (see figure 1 and table 1).

The application belongs to the technical field of microorganisms, and discloses application of shikimic acid in preparation of antibacterial preparations for inhibiting staphyloxin synthesis. It is found that shikimic acid can inhibit synthesis of staphyloxin in methicillin-resistant Staphylococcus aureus (MRSA), reduce tolerance of the bacteria to oxidative stress, induce accumulation of intracellular active oxygen, and damage cell membrane structure and function, including reducing cell membrane order, inducing membrane depolarization, increasing membrane permeability, and leading to ATP leakage and energy metabolism disorder. A new strategy and preparation direction are provided for clinical treatment of multi-drug resistant Staphylococcus aureus infection, and the application has important theoretical value and application prospect.

The application provides a genetically engineered bacterium for promoting production of phenazine-1-carboxylic acid by enhancing a synthesis pathway, and uses a green needle Pseudomonas aeruginosa genetically engineered bacterium QPCA Delta DTH as a starting strain, introduces an aroC gene into a site where a hppA gene is knocked out in the green needle Pseudomonas aeruginosa genetically engineered bacterium QPCA Delta DTH to obtain a strain QPCA Delta DTH:C; and / or introduces a phzIR gene into a Detg gene site of the strain QPCA Delta DTH:C to obtain a QPCA Delta DTH:C:IR genetically engineered bacterium. The aroC gene and the phzIR gene are integrated into the genome to enhance a phenazine synthesis precursor pathway, a shikimic acid pathway and a phenazine synthesis pathway, so that the yield of phenazine-1-carboxylic acid of the initial strain is increased from 4787.2 mg / L to 7746 mg / L.

The invention relates to application of shikimic acid in jujube tree field callus induction regeneration buds. Wherein the concentration of the shikimic acid is 20 to 60 mg / L; adding the shikimic acid into an inducer for use; in the process of inducing regenerated buds by calluses in the field of jujube trees, the shikimic acid can increase the budding number of the regenerated buds. By screening a large number of compounds, it is found that when shikimic acid is added into the inducer, the budding number of jujube tree field callus induced regeneration buds can be greatly increased, and a solid foundation is laid for polyploid breeding and transgenic breeding of jujube tree field callus.

The present disclosure discloses a method for constructing a chicory poria complex fingerprint, including (1) preparing a sample solution of a chicory poria complex, (2) preparing a mixed control solution using chlorogenic acid, 6'-O-β-D-glucosylgentiopicroside, swerthiamarin, gentiopicroside, swelloside, 3-O-caffeoylshikimic acid, neoastilbin, astilbin, rutin, isoastilbin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside, and narcissine, and (3) injecting the sample solution and the mixed control solution into a high-performance liquid chromatograph, respectively, and performing a similarity analysis on the resulting chromatograms to obtain a chicory poria complex fingerprint. The present disclosure also provides a chicory poria complex fingerprint constructed by the construction method and its application to identifying and / or evaluating the quality of chicory poria complexes. The disclosed chicory poria compound fingerprint can comprehensively, objectively, and accurately detect and evaluate the quality of chicory poria compound, thereby ensuring safe and effective medication for patients.

This invention discloses a genetically engineered bacterium for synthesizing protocatechuic acid and its applications, belonging to the field of gene recombination technology. The genetically engineered bacterium for synthesizing protocatechuic acid disclosed in this invention is a recombinant *Corynebacterium glutamicum* strain based on *Corynebacterium glutamicum* ATCC13032, or a *Corynebacterium glutamicum* strain modified with a chassis microbial substrate, including the shikimic acid pathway; and / or the DAHP pathway; and / or the 4-HBA pathway. The highest concentration of protocatechuic acid obtained by fermentation of the recombinant *Corynebacterium glutamicum* constructed in this invention can reach 85.16 g / L, which is the highest yield of protocatechuic acid synthesized de novo by biological methods reported in related studies to date. This has great potential for the industrial application of high-yield protocatechuic acid by biological methods. The genetically engineered bacterium constructed in this invention is safe and non-toxic, and can efficiently produce protocatechuic acid using glucose as a substrate through microbial fermentation. Furthermore, the culture medium composition is simple, batches are stable, and production costs are low.

The invention relates to a polyolefin adhesive for stainless steel and a preparation method thereof, and belongs to the technical field of adhesives. According to the invention, quinic acid and shikimic acid are firstly used for modifying sodium carbonate, then modified calcium carbonate and ramie fiber are used as fillers for filling so as to construct an efficient stress transfer system, and meanwhile, maleic anhydride grafted polyethylene is also added for assisting filling of the fillers, so that the impact resistance of the prepared adhesive is effectively improved; besides, malonaldehyde is added to reduce the activity of molecules in the material and limit molecular motion, vinyl neodecanoate with a side group is doped to increase the steric hindrance of molecular motion in the system, and hexanediol diglycidyl ether is doped to increase the density of a cross-linked network of the system, so that the composite material has excellent mechanical properties. The adhesive prepared by the method has relatively high peel strength.

The present invention pertains to a method for producing an antimicrobial composition preferably comprising piceol. Moreover, the invention relates to an antimicrobial composition comprising or consisting of piceol, preferably in an excess amount compared to picein, further plant-derived substances and optionally picein. The invention further relates to a cosmetic, personal care, household, home care, pet care or pharmaceutical product comprising said antimicrobial composition. Moreover, the invention relates to the use of a microorganism, preferably a lactobacillus species, for producing an antimicrobial composition comprising piceol from a composition, preferably a plant-extract, comprising picein. In addition, the invention relates to the use of shikimic acid for increasing the antimicrobial efficacy of piceol and / or to the use of piceol for increasing the antimicrobial efficacy of shikimic acid. Further embodiments and aspects of the invention are presented in the attached claims as well as in the description and examples below.

The invention provides a patchouli alcohol glucoside derivative as well as a preparation method and application thereof, the structural formula of the patchouli alcohol glucoside derivative is shown as a formula I. The invention also provides a preparation method of the patchouli alcohol glucoside derivative and application of the patchouli alcohol glucoside derivative in preparation of antidepressant drugs. The dissolution rate of the patchouli alcohol glucoside derivative in water is increased by more than 1000 times compared with that of patchouli alcohol, pharmacological tests prove that the antidepressant activity of the patchouli alcohol glucoside derivative is obviously higher than that of patchouli alcohol, the patchouli alcohol glucoside derivative can be used for preparing antidepressant drugs, and a new choice is provided for the antidepressant drugs: in the formula, R is selected from any one of glucoside, rhamnoside and moss phenol glucoside.

The application discloses a genetically engineered bacterium with high yield of gallic acid and application thereof, and belongs to the technical field of gene recombination.The genetically engineered bacterium with high yield of gallic acid is prepared from Yarrowia lipolytica ATCC MYA-2613 as a starting strain, and the ku70 gene is knocked out; the gene coding 4-hydroxyphenylpyruvate dioxygenase is expressed; the shikimic acid pathway is strengthened; and three copies of tHMGR and the lysine defect are integrated.In 2L fermenter fed-batch fermentation, the yield of gallic acid is 33.71g / L, which is more than 10 times higher than the highest yield of gallic acid of 2.8g / L of the genetically engineered Yarrowia lipolytica reported at present in 2L fermenter fed-batch fermentation, and the application lays a solid foundation for industrialization of Yarrowia lipolytica for producing gallic acid, and has an industrial application prospect.

The invention discloses a genetically engineered bacterium for high yield of uromelanoic acid and application thereof, and belongs to the technical field of gene recombination. The invention discloses a genetically engineered bacterium for high yield of uromelanoic acid, which is characterized in that yarrowia lipolytica ATCC MYA-2613 is used as an original strain, and a ku70 gene is knocked out; the method comprises the following steps: expressing a gene for coding 4-hydroxyphenylpyruvate dioxygenase; intensifying a shikimic acid pathway; and the tHMGR of three copies is integrated, and leucine defects are supplemented. The Yarrowia lipolytica genetically engineered bacterium has the advantages that the Yarrowia lipolytica genetically engineered bacterium has the advantages that the Yarrowia lipolytica genetically engineered bacterium is high in yield, the yield of the uromelanic acid is 33.71 g / L when the Yarrowia lipolytica genetically engineered bacterium is subjected to fed-batch fermentation in 2L fermentation tanks and is increased by more than 10 times as compared with the highest yield 2.8 g / L of the uromelanic acid when the Yarrowia lipolytica genetically engineered bacterium is subjected to fed-batch fermentation in the 2L fermentation tanks, and solid foundations are laid for industrialization of Yarrowia lipolytica

This invention belongs to the field of feed additive technology, and particularly relates to a feed additive that reduces antibiotic resistance genes in animal feces, its preparation method, and its application. The feed additive comprises the following components in the indicated mass ratios: rosmarinic acid 10-15%, 5-O-caffeoylshikimic acid 10-15%, Lycopus lucidus flavonoids 8-12%, tea polyphenols 7-10%, DL-α-tocopherol 7-10%, hydroxypropyl-β-cyclodextrin 22-28%, microcapsule wall material 18-22%, EDTA-2Na 0.8-1.2%, and citrate-sodium citrate buffer 1.5-2.2%. This feed additive not only exhibits clear antibacterial activity against Escherichia coli and Salmonella, but also improves the growth performance of weaned piglets, reduces the diarrhea rate in weaned piglets, and decreases the abundance of antibiotic resistance genes in their feces, showing promising application prospects.

The invention discloses an exopolysaccharide-based multibenefit probiotic microcapsule with a core-shell structure and a preparation method of the exopolysaccharide-based multibenefit probiotic microcapsule. The microcapsule inner core is formed by crosslinking probiotic exopolysaccharides, vitamin B3 and iron ions and is used for embedding probiotics; the shell is formed by compounding sodium alginate and probiotic exopolysaccharides, and is subjected to ionic gel curing molding by dropwise adding a calcium chloride solution. According to the method, the encapsulation efficiency of the probiotics can be remarkably improved, and the microcapsules are endowed with good pH response release characteristics, so that the probiotics are effectively protected, and targeted release of the probiotics in intestinal tracts is realized. In addition, in the system, the probiotic exopolysaccharide further has an anti-inflammatory effect, vitamin B3 can promote metabolism of probiotics to generate shikimic acid, the overall anti-inflammatory effect of the probiotic microcapsule is further enhanced, and iron ions further have the physiological function of effectively preventing and improving anemia. The microcapsule prepared by the invention has a wide application prospect in the fields of functional foods and probiotic preparations.