77 results about "Shikimic acid" patented technology

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The invention relates to a yarrowia lipolytica strain for producing tyrosol as well as a construction method and application of the yarrowia lipolytica strain, and belongs to the technical field of genetic engineering. According to the present invention, ScADH6, ScARO10F138L and D218G genes are expressed in the yarrowia lipolytica W29 [delta] KU70; the shikimic acid feedback inhibition is relieved by expressing the Y1ARO3K225L gene, the Y1ARO4K2214 gene and the Y1AR07G139S gene; pmLAAD, EcTyrAM53I and A354V genes are introduced, so that the conversion from FAD to FADH2 is promoted, and the supply of NADH (Nicotinamide Adenine Dinucleotide Hormone) in cytoplasm is enhanced; a 4-hydroxyphenylpyruvate dioxygenase (HPD) gene is knocked out; and overexpressing Y1DHS1, Y1DHS2 and Y1DHS3 genes of a shikimic acid pathway to obtain the yarrowia lipolytica strain HY5 for producing tyrosol. The invention lays a foundation for industrial production of tyrosol, and has important social significance and economic value.

The invention discloses a cistanche deserticola extract for improving bone mineral density, the total content of echinacoside and verbascoside in the cistanche deserticola extract is greater than 25wt%, and the cistanche deserticola extract is obtained by adding distilled water into cistanche tubulosa coarse powder for extraction, filtration, filtrate centrifugation, concentration, extraction, concentration and impurity removal, dilution, after-treatment, concentration and drying. The total content of echinacoside and verbascoside in the cistanche deserticola extract for improving the bone mineral density is 25-30 wt%, and the cistanche deserticola extract is rich in shikimic acid, phenylpropionic acid and alkaloid components, so that the cistanche deserticola extract can stably play a role in improving the bone mineral density.

The invention relates to the technical field of preparation of L-tyrosine, and discloses a method for enhancing generation of L-tyrosine in nicotiana benthamiana and application. In order to relieve product negative feedback inhibition of a key enzyme in a production path of L-tyrosine in tobacco and to improve the yield of L-tyrosine in tobacco, DAHPS enzyme derived from microorganisms is subjected to L175Q site mutation and then is connected with a signal peptide AtRs1A; shikimic acid mutase / prebenzoate dehydrogenase TyrAfbr and tyrosine aminotransferase TyrB which are derived from microorganisms are introduced into the tobacco chassis after being connected with the signal peptide Rs1A, the expression has orthogonality, and transient expression is performed in the Bensi tobacco plant chassis by utilizing agrobacterium infection, so that the synthesis capability of L-tyrosine in tobacco chassis cells can be improved; a large amount of L-tyrosine can be directly generated in the tobacco chassis plastid.

The present application relates to the technical field of synthetic biology, in particular to a multi-module synergistic optimization of brown cyanidin and brevifolin production of saccharomyces cerevisiae engineering bacteria and its construction method and application. The saccharomyces cerevisiae engineering bacteria includes naringenin synthesis pathway, optimization pathway of shikimic acid, synergistic pathway of phenylalanine, 6-OH luteolin synthesis pathway, brown cyanidin and brevifolin synthesis pathway, multiple copies of SbF6H and MpalOMT2, NADPH optimization pathway, and SAM cycle optimization pathway. The saccharomyces cerevisiae engineering bacteria is used for producing brown cyanidin and brevifolin, and improving the yield of the two.

The invention discloses a waste lithium ion battery recycling method, and belongs to the technical field of recycling of waste lithium ion batteries. The method comprises the following steps: leaching the positive electrode powder of the waste lithium ion battery in a deep eutectic solvent, and carrying out solid-liquid separation to obtain a leaching solution rich in heavy metals; the eutectic solvent is prepared from shikimic acid, catechol and tetradecyl dimethyl benzyl ammonium chloride. The deep-eutectic solvent is adopted for leaching, the leaching rate of nickel, cobalt, manganese and other heavy metals can be increased, meanwhile, leaching of impurity metals is avoided, and then it is avoided that a large amount of waste water is generated due to impurity removal. The metal ions and the eutectic solvent are separated by using the ion exchange resin, so that the eutectic solvent is recovered and can be repeatedly used, and the pure metal solution is obtained.

This invention relates to the field of traditional Chinese medicine analysis, and particularly to a method for determining the content of multiple components in Terminalia chebula and constructing its characteristic fingerprint spectrum. The method provided by this invention can simultaneously determine chebulin, gallic acid, 4-galloshikimic acid, 5-galloshikimic acid, punicalin (punicalin A&B), corilagin, 1,3,6-tri-O-galloyl-β-D-glucose, chebulin, urolithin M5, chebulin tannic acid, chebulin-lintannic acid, and ellagic acid in Terminalia chebula. It exhibits high sensitivity, good precision, repeatability, and stability, and can be used to detect the tannin content in the extract at each step of the tannin extraction process, as well as for related detection in the study and quality evaluation of tannin components in Terminalia chebula. It can also be used to construct the characteristic fingerprint spectrum of Terminalia chebula. This method can also be used for determining the content of tannin compounds and constructing their characteristic fingerprint spectrum in Terminalia chebula, which contains similar tannin compounds to Terminalia chebula.

The invention discloses a drug-loaded nanoparticle as well as a preparation method and application thereof. The drug-loaded nanoparticle can be used for treating triple negative breast cancer, and specifically comprises a magnetic nanoparticle, an active component loaded in the magnetic nanoparticle, and an erythrocyte membrane coating the magnetic nanoparticle, wherein the active ingredients are selected from one or more of piperlongumine, polyphyllin II, erianin, pseudolaric acid, 6-gingerol, ginkgetin, solasonine, cucurbitacine B and lycorine. In practical application, the drug-loaded nanoparticles have good biological safety, long circulation capability and high tumor retention capability, and can generate efficient killing and treatment effects on triple-negative breast cancer by inducing cell apoptosis.

The application discloses a kind of recombinant escherichia coli for producing L-tyrosine and application, belong to genetic engineering and bioengineering technical field.The application knocks out aroP and tyrP gene, expresses the yddG gene of escherichia coli endogenous to modify the aromatic amino acid transport system of escherichia coli, then heterologous expression comes from bifidobacterium fpk and expresses the ppsA and tktA gene of escherichia coli endogenous to increase the precursor supply of shikimic acid pathway, again through the anti feedback gene expression aroG and tyrA 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase, branch acid mutase and prephenate dehydrogenase are removed feedback inhibition, increase the carbon flux of shikimic acid pathway.Knock out tyrR gene removes the repression of TyrR protein to shikimic acid pathway key enzyme, again knock out trpE and pheA gene to block partial side reaction of shikimic acid pathway, so that the synthesis flux of L-tyrosine increases.Finally knock out endogenous poxB gene to realize the modification of acetic acid pathway, realizes the stable fermentation performance of high glucose concentration.

The invention relates to a composition comprising shikimic acid and an extract of Prunus Mume, and its non-medical and medical use, in particular for use in the treatment of acne.

The application discloses a Pichia pastoris engineering bacterium for converting methanol to synthesize bakuchiol from scratch as well as construction and application of the bacterium. In different chromosomal neutral sites of the Pichia pastoris, a heterologous p-coumaric acid synthesis pathway is introduced, key genes of tyrosine and phenylalanine synthesis pathways of a heterologous shikimic acid pathway are knocked out, overexpressed or heterologously expressed, a high-yield p-coumaric acid strain is obtained, on the basis of the strain, a heterologous bakuchiol biosynthesis pathway is introduced, bakuchiol synthetase is overexpressed, key genes of a MVA pathway and genes of an exogenous acetyl coenzyme A supply pathway are endogenously overexpressed or heterologously expressed, after optimization of a methanol concentration and back complementation of a His4 gene of the strain, the yield of the bakuchiol is effectively increased to 91.2 mg / L, which is 59.8 times higher than that of an initial strain, and the yield of the bakuchiol in a 15L fermenter reaches 692.8 mg / L. The application has the characteristics of high conversion efficiency, low production cost, easy preparation, wide industrial application prospect and the like.

The invention discloses escherichia coli capable of producing sakuranetin as well as a construction method and application of the escherichia coli. The escherichia coli capable of producing sakuranetin is introduced into a sakuranetin de novo synthesis path, a shikimic acid metabolism path is further reconstructed, 87.47 mg / L sakuranetin can be synthesized by fermentation in a shake flask only with glucose as a substrate, and the yield is the highest yield in currently reported escherichia coli. The invention further provides a method for producing sakuranetin through fermentation by taking the kelp extracting solution as the substrate, 57.4 mg / L sakuranetin can be synthesized by taking the kelp extracting solution as the substrate, so that high-value conversion of food industrial wastes can be effectively realized, and the production cost can be reduced.

The invention provides a method for improving the sugar-acid conversion rate in a shikimic acid fermentation production process. Comprising the following steps: S1, respectively preparing a seed culture medium and a fermentation culture medium, wherein the fermentation culture medium contains 0.1-0.4 g / L of methyl-alpha-D-glucopyranoside; s2, a seed culture stage: sequentially inoculating the escherichia coli bottle-combining seed solution into a primary seed tank and a secondary seed tank, and respectively culturing for a period of time; s3, fermentation culture stage: supplementing the culture solution after seed culture into a three-stage fermentation tank, and controlling the pH value of the fermentation solution to be 6.7-6.9 within 0-12 hours; the pH value of the fermentation liquor is 6.4-6.6 when the fermentation liquor is discharged from 12h to the tank, and co-culturing for 42-54h to obtain the fermentation liquor containing shikimic acid. Through double means of culture medium formula design, seed culture and fermentation culture process design, the conversion rate of synthesizing shikimic acid from glucose is synergistically increased, the overall operation is simple, the dosage of used methyl-alpha-D-glucopyranoside is small, the cost is low, more shikimic acid can be converted, and for medicine production enterprises, the method has a very good application prospect. The obvious cost-reducing and effect-increasing effects are realized.

The invention discloses a method for synthesizing shikimic acid from shikimic acid dehydrogenase of a plant and microorganisms, which uses a shikimic acid synthesis method based on a shikimic acid dehydrogenase gene of the plant, and is characterized by comprising the following steps: S1, obtaining a shikimic acid dehydrogenase gene sequence from the plant; s2, performing codon optimization on the gene; s3, inserting the optimized shikimic acid dehydrogenase gene into an expression vector; s4, transferring the expression vector into escherichia coli engineering bacteria to express shikimic acid dehydrogenase; and S5, synthesizing shikimic acid through an in-vitro catalytic reaction. The screened enzyme has the advantages of few side reactions, no inhibition by high-concentration products and high activity. A recombinant strain for producing shikimic acid is obtained by utilizing the enzyme through a metabolic engineering means. The strain does not use plasmids, has strong genetic stability, does not need additional aromatic amino acids in a fermentation medium, has low production cost, high yield and yield and few metabolic byproducts, and has important industrial application value.

The present invention provides an aromatic compound composition containing at least an aromatic compound (A) having a measured bio-based content of 80% to 100% (inclusive), the measured bio-based content indicating the proportion of radioactive carbon 14C derived from a biomass resource, the measured bio-based content being determined on the basis of the concentration of radioactive carbon in circulating carbon at 1950 as measured in accordance with ASTM D6866-20, the aromatic compound (A) is phenol (A) or 4-hydroxybenzoic acid (A), and when the aromatic compound (A) is phenol (A), the aromatic compound composition is dissolved in pure water so that the concentration of the aromatic compound composition is 90 mass%, the absorbance at a wavelength of 425 nm is 0.001-0.70, and when the aromatic compound (A) is phenol (A), the absorbance at a wavelength of 425 nm is 0.001-0.70. When the aromatic compound (A) is 4-hydroxybenzoic acid (A), the aromatic compound composition has an absorbance of 0.015 or less at a wavelength of 425 nm when the aromatic compound composition is dissolved in ethanol at a concentration of 20 g / L, or when the total amount of the aromatic compound composition is 100 mass%, the absorbance is 0.015 or less at a wavelength of 425 nm. The content of shikimic acid in the aromatic compound composition is 0.012 mass% or less.

The invention relates to a hair care composition comprising malic acid, arginine and shikimic acid, and to the use of said components in the manufacture of a hair care composition.

The present invention relates to a recombinant microorganism, preferably a recombinant yeast, capable of producing caffeic acid comprising a heterologous gene coding for an enzyme of the hydrolase family capable of breaking, preferably of hydrolyzing, the caffeoyl-shikimate bond to produce caffeic acid from caffeoyl-shikimate. Said microorganism, preferably said recombinant yeast, may also be capable of producing ferulic acid from the caffeic acid obtained. The present invention also relates to a method for producing caffeic acid and a method for producing caffeic acid and / or ferulic acid, using microorganisms, preferably yeasts, according to the invention. Finally, the invention also relates to the use of microorganisms, preferably yeasts, according to the invention to produce caffeic acid and / or ferulic acid.

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral / antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral / antibacterial exceeding a 3 log 10 reduction in viral titer.

The invention relates to the technical field of synthetic biology, in particular to a multi-module collaborative optimization high-yield Jaceosidin and Eupatorin Saccharomyces cerevisiae engineering bacterium as well as a construction method and application thereof. The saccharomyces cerevisiae engineering bacteria comprise a synthetic route of naringenin, an optimized route of shikimic acid, a synergistic route of phenylalanine, a synthetic route of 6-OH luteolin, a synthetic route of jaceosidin and eupatorin, multiple copies of SbF6H and MpalOMT2, an optimized route of NADPH (nicotinamide adenine dinucleotide phosphate) and an optimized route of SAM circulation. The saccharomyces cerevisiae engineering bacterium is used for producing jaceosidin and eupatorin, and the yield of jaceosidin and eupatorin is increased.

The present invention pertains to a method for producing an antimicrobial composition preferably comprising piceol. Moreover, the invention relates to an antimicrobial composition comprising or consisting of piceol, preferably in an excess amount compared to picein, further plant-derived substances and optionally picein. The invention further relates to a cosmetic, personal care, household, home care, pet care or pharmaceutical product comprising said antimicrobial composition. Moreover, the invention relates to the use of a microorganism, preferably a lactobacillus species, for producing an antimicrobial composition comprising piceol from a composition, preferably a plant-extract, comprising picein. In addition, the invention relates to the use of shikimic acid for increasing the antimicrobial efficacy of piceol and / or to the use of piceol for increasing the antimicrobial efficacy of shikimic acid. Further embodiments and aspects of the invention are presented in the attached claims as well as in the description and examples below.

The invention discloses recombinant escherichia coli, a construction method thereof and application of the recombinant escherichia coli in production of 3-dehydroshikimic acid, and belongs to the technical field of bioengineering. According to the recombinant escherichia coli, escherichia coli FMME-DHS03 is used as an original strain, pyruvate kinase gene pykA is knocked out, excessive 3-dehydroquinic acid synthase gene aroB, transketolase gene tktA and galactose permease gene galP are added, the finally obtained recombinant strain is fermented for 40 h, the yield of 3-dehydroshikimic acid reaches 130.9 g / L, and the yield of glucose reaches 0.55 g / g. The process for producing the 3-dehydroshikimic acid through fermentation is simple and easy to operate, free of additional antibiotics, short in period, high in yield, low in culture medium cost and suitable for industrial production.

The application discloses a Yarrowia lipolytica strain for synthesizing (2S)-naringenin and application thereof, and belongs to the field of genetic engineering and bioengineering technology. The application improves the yield of (2S)-naringenin by 60% through semi-rational modification of a key enzyme CHS, and further integrates the modified CHS into the yeast genome. In addition, the application promotes the conversion of coumaric acid to (2S)-naringenin by strengthening the shikimic acid pathway and enhancing the supply of auxiliary factors, so that the yield of (2S)-naringenin reaches 776 mg / L after 96 hours of fermentation of the constructed strain in a 24-hole plate, and the yield reaches 8.65 g / L in a 5L fermenter. The application lays a foundation for subsequent biosynthesis of flavonoids, and has potential value and significance for the development of synthetic biology.

The invention discloses a selective reduction method of 5-O-methylsulfonyl ethyl shikimic acid ketal (acetal), which can be used for preparing a Tamiflu key intermediate (Roch epoxy intermediate). A series of ketal (acetal) compounds I-1-10 and a reducing agent Et3SiH are subjected to selective reduction reaction under mild conditions under the action of a catalyst AlCl3 to obtain C-3 allylic position various substituted ether compounds II-1-10, and the yield is 65-97% (see figure 1 and table 1).

The application belongs to the technical field of microorganisms, and discloses application of shikimic acid in preparation of antibacterial preparations for inhibiting staphyloxin synthesis. It is found that shikimic acid can inhibit synthesis of staphyloxin in methicillin-resistant Staphylococcus aureus (MRSA), reduce tolerance of the bacteria to oxidative stress, induce accumulation of intracellular active oxygen, and damage cell membrane structure and function, including reducing cell membrane order, inducing membrane depolarization, increasing membrane permeability, and leading to ATP leakage and energy metabolism disorder. A new strategy and preparation direction are provided for clinical treatment of multi-drug resistant Staphylococcus aureus infection, and the application has important theoretical value and application prospect.

The invention relates to the use of shikimic acid in the manufacture of a cleaning composition and a cleaning composition comprising 0.001-0.250% wt. of said shikimic acid and wherein the pH of said cleaning composition is in the range of 2.5-12.

The application provides a genetically engineered bacterium for promoting production of phenazine-1-carboxylic acid by enhancing a synthesis pathway, and uses a green needle Pseudomonas aeruginosa genetically engineered bacterium QPCA Delta DTH as a starting strain, introduces an aroC gene into a site where a hppA gene is knocked out in the green needle Pseudomonas aeruginosa genetically engineered bacterium QPCA Delta DTH to obtain a strain QPCA Delta DTH:C; and / or introduces a phzIR gene into a Detg gene site of the strain QPCA Delta DTH:C to obtain a QPCA Delta DTH:C:IR genetically engineered bacterium. The aroC gene and the phzIR gene are integrated into the genome to enhance a phenazine synthesis precursor pathway, a shikimic acid pathway and a phenazine synthesis pathway, so that the yield of phenazine-1-carboxylic acid of the initial strain is increased from 4787.2 mg / L to 7746 mg / L.

The invention relates to application of shikimic acid in jujube tree field callus induction regeneration buds. Wherein the concentration of the shikimic acid is 20 to 60 mg / L; adding the shikimic acid into an inducer for use; in the process of inducing regenerated buds by calluses in the field of jujube trees, the shikimic acid can increase the budding number of the regenerated buds. By screening a large number of compounds, it is found that when shikimic acid is added into the inducer, the budding number of jujube tree field callus induced regeneration buds can be greatly increased, and a solid foundation is laid for polyploid breeding and transgenic breeding of jujube tree field callus.

The present disclosure discloses a method for constructing a chicory poria complex fingerprint, including (1) preparing a sample solution of a chicory poria complex, (2) preparing a mixed control solution using chlorogenic acid, 6'-O-β-D-glucosylgentiopicroside, swerthiamarin, gentiopicroside, swelloside, 3-O-caffeoylshikimic acid, neoastilbin, astilbin, rutin, isoastilbin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside, and narcissine, and (3) injecting the sample solution and the mixed control solution into a high-performance liquid chromatograph, respectively, and performing a similarity analysis on the resulting chromatograms to obtain a chicory poria complex fingerprint. The present disclosure also provides a chicory poria complex fingerprint constructed by the construction method and its application to identifying and / or evaluating the quality of chicory poria complexes. The disclosed chicory poria compound fingerprint can comprehensively, objectively, and accurately detect and evaluate the quality of chicory poria compound, thereby ensuring safe and effective medication for patients.

This invention discloses a genetically engineered bacterium for synthesizing protocatechuic acid and its applications, belonging to the field of gene recombination technology. The genetically engineered bacterium for synthesizing protocatechuic acid disclosed in this invention is a recombinant *Corynebacterium glutamicum* strain based on *Corynebacterium glutamicum* ATCC13032, or a *Corynebacterium glutamicum* strain modified with a chassis microbial substrate, including the shikimic acid pathway; and / or the DAHP pathway; and / or the 4-HBA pathway. The highest concentration of protocatechuic acid obtained by fermentation of the recombinant *Corynebacterium glutamicum* constructed in this invention can reach 85.16 g / L, which is the highest yield of protocatechuic acid synthesized de novo by biological methods reported in related studies to date. This has great potential for the industrial application of high-yield protocatechuic acid by biological methods. The genetically engineered bacterium constructed in this invention is safe and non-toxic, and can efficiently produce protocatechuic acid using glucose as a substrate through microbial fermentation. Furthermore, the culture medium composition is simple, batches are stable, and production costs are low.