772 results about "Elution" patented technology

The invention discloses an online component detection method and system based on a salvia miltiorrhiza extracting solution chromatography elution process, and relates to the technical field of traditional Chinese medicine quality control. The method comprises the following steps: carrying out an elution experiment on a salvia miltiorrhiza extracting solution based on a column chromatography technology to obtain a sample set; acquiring near infrared spectrum and ultraviolet visible spectrum data in the sample; matching a spectrum fusion model according to the type of the to-be-detected component, and constructing different spectrum fusion models based on different fusion strategies for the near infrared spectrum and the ultraviolet visible spectrum; and processing data of the near infrared spectrum and the ultraviolet visible spectrum by using different spectrum fusion models to obtain the content of the to-be-detected component. On the basis of NIRS and UV-Vis technologies, online detection and analysis are carried out in the elution process of the salvia miltiorrhiza extracting solution by adopting a spectrum fusion and water spectrum omics method, and dynamic tracking and intelligent terminal point judgment of the salvia miltiorrhiza water-soluble active ingredients are realized.

The invention discloses an efficient separation and purification method of MLnM type medium-chain and long-chain triglyceride, and belongs to the technical field of separation and purification. The efficient separation and purification method comprises the following steps: S1, preparing a crude product; s2, mixing the crude product with an adsorbent for static adsorption; and S3, performing column chromatography elution on the static adsorption product, and performing dynamic chromatography to separate the MLnM type triglyceride. According to the efficient separation and purification method of the MLnM type medium-long-chain triglyceride, a neutral aluminum oxide static adsorption and dynamic column chromatography coupling process is adopted, the purity of the MLnM type triglyceride can be greatly improved and is far higher than that of other adsorbents, the whole process steps are clear, the steps of raw material preparation, static adsorption, dynamic chromatography and the like are included, and the method is suitable for industrial production. The operation process is easy for standardized and large-scale production, and does not need to depend on complex equipment such as molecular distillation, supercritical CO2 extraction and the like.

A method for quality control checking a gas chromatography apparatus at a rig site, the GC apparatus including at least a main column and a makeup flow line in fluid communication with a detector manifold. The GC apparatus measures a first chromatogram of a gas sample with a first makeup gas flow rate and a second chromatogram of the gas sample with a second makeup gas flow rate, wherein the second makeup gas flow rate is greater than the first makeup gas flow rate. The elution times of at least one common peak in the first and second chromatograms are compared to indicate a leak or an obstruction in the makeup flow line or the detector manifold.

The invention discloses a system and a method for rapidly measuring trace F <-> and Cl <-> in water based on chromatographic gradient elution. The system comprises a water storage tank, an anion trapper, an eluent generator, a six-way sample injection valve, a guard column, an analytical column, a suppressor, a conductivity detector and a chromatographic workstation, an outlet of the water storage tank is communicated with an inlet of the leacheate generator through the anion trapper, an outlet of the leacheate generator is communicated with an inlet of the guard column through the six-way sample injection valve, and an outlet of the guard column is communicated with the conductivity detector through the analytical column and the suppressor. The chromatographic work station is connected with the leacheate generator, the six-way sample injection valve, the suppressor and the conductivity detector, and the system and the method can realize rapid measurement of strongly corrosive anions F <-> and Cl <-> in water and rapid elution of other anions, so that the measurement time of each sample is reduced by at least 40% or more.

The invention provides a device and a method for detecting antibiotics in aquaculture water, and belongs to the technical field of environmental pollutant detection. According to the device disclosed by the invention, a Fe3O4 (at) GO magnetic nano adsorption material is packaged in a micro-fluidic chip reaction chamber, and a magnetic sticker is innovatively adopted to realize immobilization and replaceable design of an adsorbent; the integrated electromagnetic oscillation module drives the adsorbent to vibrate in the microchannel, and the mass transfer capture efficiency of antibiotics such as sulfonamides, quinolones and tetracyclines is remarkably improved. According to the method, an adsorption-elution-detection integrated process is initiated for the first time, the pretreatment time is shortened to be within 10 minutes from the hour level, synchronous detection of 0.01-5 [mu] g / L ultra-trace multi-component antibiotics in 5-10 [mu] L of a water sample is achieved, compared with a traditional method, the detection precision is remarkably improved, the matrix interference resistance is high, and the method is suitable for large-scale popularization and application. The method is suitable for real-time online monitoring of multiple antibiotic residues in the aquaculture environment.

The invention discloses an online pre-column derivatization coupled liquid chromatography-mass spectrometry amino acid detection method, and relates to the technical field of analysis and detection, and the method comprises the following steps: obtaining a to-be-detected sample, and carrying out protein hydrolysis treatment to obtain hydrolysate; mixing the hydrolysate with a derivatization reagent, carrying out on-line derivatization reaction through multiple sections of reaction zones which are connected in series, and maintaining different pH environments in the reaction zones; an online detection unit is arranged at an outlet of the reaction zone to monitor the concentration of derivative products and adjust reaction conditions in real time; performing chromatographic separation on the derivative reaction liquid; switching an ion source ionization polarity mode according to an elution time window to carry out mass spectrometry detection; and substituting the mass spectrum response signal into the standard curve to calculate the amino acid concentration value. The method realizes automatic derivation and high-sensitivity detection of amino acid, and has the advantages of simplicity and convenience in operation, controllable reaction conditions and high detection efficiency.

The invention relates to the technical field of medical detection, in particular to a method for detecting aldosterone, cortisol, deoxycorticosterone and cortisone in plasma through LC-MS / MS. The method comprises the following steps: (a) mixing a plasma sample with an internal standard solution to obtain a mixed solution; (b) adding the mixed solution into the magnetic bead suspension, sequentially carrying out adsorption, weak washing, strong washing and elution, and collecting an eluent; (c) carrying out liquid nitrogen blowing and redissolving on the eluent, and then carrying out LC-MS / MS detection; wherein the surfaces of the magnetic beads are modified with steroid adsorption groups, and the target detection objects are aldosterone, cortisol, deoxycorticosterone and cortisone. The detection method is convenient and simple, the detection efficiency is higher than that of a traditional method, and a feasible solution is provided for promoting popularization and application of the LC-MS / MS technology in clinical practice.

The invention relates to the technical field of rare earth element separation methods, in particular to a method for separating ytterbium and lutetium by using a phosphate adsorbent, which comprises the following steps: loading a phosphate solution on a porous solid-phase carrier by using a vacuum impregnation method, and carrying out reduced pressure evaporation and drying to obtain a phosphate solid adsorbent; filling an exchange column with the solid adsorbent to obtain the exchange column filled with the solid adsorbent; enabling a nitric acid solution containing ytterbium and lutetium to flow through an exchange column filled with a solid adsorbent to complete a column adsorption process; and performing elution, performing desorption by using a desorption solution to obtain the regenerated exchange column filled with the solid adsorbent, respectively collecting the ytterbium solution and the lutetium solution, completing the separation process, and repeating the steps. By adopting the steps, the separation of ytterbium and lutetium is directly realized from the nitric acid solution containing ytterbium Yb and lutetium Lu through column adsorption separation, leaching and desorption processes, and high-purity ytterbium and lutetium products are prepared.

The invention relates to the field of water quality monitoring of a secondary loop of a nuclear power plant, in particular to a synchronous detection system and method for ammonia and hydrazine. The method comprises the following steps: removing impurities in a sample by utilizing filtration and reverse chromatography; the sample is quantified to 240-260 [mu] L; carrying out gradient elution on the sample by using a methanesulfonic acid solution as an eluent; wherein in the first stage, the concentration of a methanesulfonic acid solution is controlled to be 13-18 mmol / L, and a sample is washed; in the second stage, the concentration of the methanesulfonic acid solution is controlled to be 55-70 mmol / L, and the sample is Drawing a low-concentration curve based on the 10-500 [mu] g / L standard solution, and drawing a high-concentration curve based on the 500-3000 [mu] g / L standard solution; and measuring the ionic conductivity values of NH4 < + > and N2H5 < + >, automatically selecting a corresponding concentration curve according to the peak area of the ionic conductivity values, and calculating the concentration of ammonia and hydrazine. According to the invention, the synchronous detection of ammonia and hydrazine concentration is realized, the analysis process is simplified, the use of toxic and harmful substance phenol and the generation of toxic and harmful waste liquid of yellow azo compound are solved, the measurement accuracy is improved, and the relative measurement error can be controlled within + / -2.0%.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a method for determining the content of five flavonoid components in a hypericum perforatum medicinal material. The content determination method comprises the following steps: preparing rutin, hyperoside, isoquercitrin, quercitrin and quercetin into a mixed reference solution; extracting a hypericum perforatum medicinal material by using an extraction solvent to prepare a test solution; the mixed reference substance solutions and the test solution with different volumes are subjected to high performance liquid chromatography respectively, a mobile phase A is acetonitrile, a mobile phase B is a phosphoric acid aqueous solution with the volume concentration of 0.08-0.12%, and elution gradient is adopted. The content determination method has the advantages of high sensitivity, simplicity and convenience in operation, low cost and high detection speed.

The invention provides low-molecular-weight tea polysaccharide and a preparation method and application thereof, and belongs to the technical field of tea polysaccharide extraction.The preparation method of the low-molecular-weight tea polysaccharide comprises the following steps that 1, tea powder, compound enzyme and water are mixed to obtain an enzymolysis system, and enzymolysis is conducted to obtain enzymatic hydrolysate; 2) performing ultrasonic-microwave synergistic extraction on the enzymatic hydrolysate, and then boiling and extracting to obtain an extracting solution; (3) inoculating compound microorganisms into the extracting solution, and fermenting to obtain fermentation liquor; 4) performing ultrafiltration and concentration on the fermentation liquor to obtain a tea polysaccharide solution; 5, the tea polysaccharide solution is subjected to affinity membrane chromatography and elution, eluent is subjected to reverse filtration through a nanofiltration membrane, trapped fluid is collected, and the low-molecular-weight tea polysaccharide is obtained.The molecular weight of the low-molecular-weight tea polysaccharide ranges from 2 KDa to 5 KDa, and the low-molecular-weight tea polysaccharide can be more easily absorbed by the human body and has the effect of rapidly and remarkably adjusting blood sugar.

The invention relates to the field of quality detection of traditional Chinese medicine preparations and specifically provides a method for constructing a characteristic spectrum of nardostachys radix and a preparation thereof and a quality detection method. In the characteristic spectrum construction method, chromatographic conditions include: using acetonitrile and an aqueous solution containing formic acid as mobile phases for gradient elution, the gradient elution program includes: 0-5 min-40 min-50 min, and the volume percentage of acetonitrile in the mobile phase is: 10%-10%-35%-50%. Under the specific elution program, 9 common characteristic peaks are obtained, and good separation of the common characteristic peaks is achieved. The obtained characteristic spectrum has a stable baseline, good characteristic peak shape, good separation effect, and strong characteristic component specificity. The method provides a basis for quality detection and control of nardostachys radix and a preparation thereof, achieves characterization of the overall components of nardostachys radix and a preparation thereof, and has stable reproducibility.

The invention discloses a method for determining bedaquiline fumarate clean verification target residues, and relates to the technical field of instrument analysis of chemical pharmacy. The method comprises the following steps: preparing a sample wiping solution; determining the content of bedaquiline fumarate in the sample wiping solution by adopting a liquid chromatography; chromatographic conditions are as follows: a chromatographic column is a VP-ODS C18 column; mobile phases: a potassium hexafluorophosphate aqueous solution and acetonitrile, wherein the concentration of the potassium hexafluorophosphate aqueous solution is 0.01-0.03 mol / L; the elution mode is isocratic elution; the flow rate is 1 ml / min to 2 ml / min; the column temperature is 28-32 DEG C; the method has good specificity and sensitivity for determination of bedaquiline fumarate cleaning verification target residues, is simple and convenient to operate, rapid in determination, simple and easy to implement, and overcomes the defects that TOC, chemical titration methods, conductivity methods, pH methods and the like which are commonly used at present do not have specificity.

The invention relates to the field of analysis and detection, and discloses a method for detecting multiple chelating agents in daily chemical products, which comprises the following steps: methylating a to-be-detected sample by using a diazomethane methylation reagent to obtain a to-be-detected solution; carrying out liquid chromatography-mass spectrometry detection on the to-be-detected liquid by adopting a specific elution program to obtain a chromatogram; and performing qualitative and / or quantitative analysis on the chelating agent in the to-be-detected sample according to the chromatogram. By adopting the detection method disclosed by the invention, efficient detection of various chelating agents (including DTPA, DTPMP, EDTMPA, HDTEPA, EGTA, HEDP, EDTA, NTA, GLDA, PBTC, MGDA, IDS, HEEDTA, HPAA, DTPA-OH, CA and ATMP) can be realized, meanwhile, interference of other components in daily chemical products on detection can be avoided, and relatively high detection sensitivity and accuracy of the chelating agents can be realized.

The present application belongs to a kind of medical radioisotope preparation field, specifically related to a kind of method for extracting lead-212 and bismuth-212 in thorium-232 decay chain, solution containing thorium-232 decay chain material is passed through anion exchange resin column, the solution containing thorium-232 decay chain material includes halogen ion, and the concentration of hydrogen ion in the solution containing thorium-232 decay chain material is greater than 0.01mol / L, lead-212 and bismuth-212 are absorbed by anion exchange resin column, and other nuclides directly pass through anion exchange resin column and are not absorbed, lead-212 and bismuth-212 are desorbed from anion exchange resin column by elution reagent, and lead-212 and bismuth-212 are separated and extracted;The present application can extract lead-212 and bismuth-212 simultaneously, and the extraction speed is fast, the purity is high, and the product is not easy to cause organic pollution.

The invention belongs to the technical field of data processing, and discloses an elution behavior prediction method and system, terminal equipment and a storage medium, and the method comprises the following steps: collecting a target liquid separated out in a simulated column chromatography elution process for multiple times at equal time intervals; carrying out near infrared spectrum collection on the obtained simulated collection samples in batches and carrying out preprocessing, training a long-short-term memory network combination model by using a sliding time window mechanism and using a plurality of batches of obtained simulated spectrum data sequenced according to time, and predicting a column chromatography elution process by using the trained model. According to the method, the key nodes in the column chromatography elution process are predicted, so that the key nodes in the elution behavior can be efficiently and accurately identified, and an operator can accurately master the elution time of a target component.

The invention relates to the technical field of analysis and detection, and discloses a construction method of a fingerprint spectrum of relevant characteristic peaks of rhizoma coptidis paste and radix rehmanniae recen, and the construction method comprises the following steps: S1, preparing a reference solution and a test solution; and S2, injecting the reference substance solution and the test solution into a high performance liquid chromatograph, adopting a chromatographic column with octadecylsilane chemically bonded silica as a filler, taking acetonitrile as a mobile phase A, taking a 0.0125 mol / L monopotassium phosphate solution as a mobile phase B, eluting according to a specified gradient program, and detecting by adopting an ultraviolet detector to obtain the fingerprint spectrum. By means of the provided construction method of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen, the sequence and the mutual relation of the characteristic peaks can be indicated, and the effect of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen is effectively represented.

The application provides a construction method and application of a mistletoe preparation characteristic spectrum, wherein the construction method of the mistletoe preparation characteristic spectrum adopts a high performance liquid chromatography method to obtain a characteristic spectrum of a to-be-detected substance; and specific chromatographic conditions of the high performance liquid chromatography method are provided, wherein an octadecylsilane bonded silica gel is used as a filler of a chromatographic column; a detection wavelength is 248-252 nm; methanol is used as a mobile phase A, and a 0.09-0.11% phosphoric acid aqueous solution is used as a mobile phase B, and elution is performed according to a specific gradient program. The characteristic spectrum obtained by the construction method is comprehensive in material information, and fully represents key characteristic chemical components of the mistletoe preparation and common adulterated water extraction preparations of the mistletoe; differences in chemical components of the mistletoe and the preparation thereof and different adulterants characterized by the method can realize overall quality control of the mistletoe and identification of adulterated mistletoe.

The invention relates to a method for detecting genotoxic impurities of rupatadine fumarate, which is characterized in that a phosphate buffer solution is matched with acetonitrile, a high performance liquid chromatography method is adopted, a chromatographic column is a silica gel bonded octadecylsilane column, a gradient program is adopted for elution, and the content of the rupatadine fumarate genotoxic impurities in the rupatadine fumarate genotoxic impurities in the rupatadine fumarate genotoxic impurities is detected. Effective separation between rupatadine fumarate and genotoxic impurities and between genotoxic impurities can be achieved, accurate quantitative analysis can be achieved, and the quality of drugs can be effectively controlled.

This invention discloses an online pre-column derivatization coupled with liquid chromatography-mass spectrometry (LC-MS) for amino acid detection, relating to the field of analytical detection technology. The method includes: obtaining a sample for protein hydrolysis to obtain a hydrolysate; mixing the hydrolysate with a derivatizing reagent and performing an online derivatization reaction through multiple reaction zones arranged in series, each zone maintaining a different pH environment; setting an online detection unit at the reaction zone outlet to monitor the concentration of the derivatized product and adjust the reaction conditions in real time; performing chromatographic separation of the derivatized reaction solution; switching the ionization polarity mode of the ion source according to the elution time window for mass spectrometry detection; and substituting the mass spectrometry response signal into a standard curve to calculate the amino acid concentration value. This invention achieves automated derivatization and high-sensitivity detection of amino acids, with advantages such as simple operation, controllable reaction conditions, and high detection efficiency.

The application discloses a crystalline hyperbranched multi-arm copolymer, a self-supporting graphene film and a preparation and application thereof. The crystalline hyperbranched multi-arm copolymer is prepared by the following method: a hyperbranched macromolecular initiator is obtained by using an alpha-diimine palladium catalyst to catalyze ethylene and a bromine-containing functional monomer in a one-step "chain removal" copolymerization in a water-free grade solvent; and then the hyperbranched macromolecular initiator is used to initiate atom transfer radical polymerization of an acrylate functional monomer to obtain the crystalline hyperbranched multi-arm copolymer. The crystalline hyperbranched multi-arm copolymer, graphite powder and a solvent are mixed in proportion, graphene initial dispersion liquid is obtained through ultrasonic and centrifugal separation, and then the self-supporting graphene film is obtained through filtration, elution and drying in sequence. The application provides an application of the self-supporting graphene film as an electrothermal film, and the self-supporting graphene film has excellent electrothermal performance and mechanical strength.

The application discloses a method for determining 15 new psychoactive substances in saliva by solid phase supported liquid-liquid extraction (SLE) combined with ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS / MS), which specifically uses a mixed solution of methyl tert-butyl ether and n-hexane as an elution solvent, adopts solid phase supported liquid-liquid extraction to pretreat saliva samples, and then adopts UPLC-MS / MS to detect the collected eluent, so as to realize qualitative and quantitative determination of the new psychoactive substances in saliva. The method has the advantages of rapidness, accuracy, high sensitivity and simple operation, the detection limit of the method is 0.2-0.5 ng / mL, can meet the domestic and foreign limit requirements, and provides technical support for monitoring and investigation of the new psychoactive substances in saliva.

A method for measuring the concentration of an analyte on the basis of a periodically variable external regulation force, and an oscillator system, which aim to solve the problem of non-specific adsorption in existing immunoassay techniques leading to reduced sensitivity and specificity in the measurement of analyte concentrations in samples. The measurement method comprises: obtaining a detection system, and setting a periodically variable external regulation force; binding both a capture probe and a detection probe with an analyte in a sample; making labeled particles, which are bound to the detection probe, perform periodic reciprocating motion under the action of the external regulation force; and by means of a monitoring and imaging apparatus, recording motion imaging signals of the labeled particles to select specifically bound molecules, and compiling statistics to calculate the proportion of the specifically bound molecules so as to obtain the analyte concentration. A specifically bound analyte can be accurately selected, thereby enhancing the capability and detection sensitivity of specific detection of analytes. The method has no complex elution processes, has a short detection response time, and exhibits high-sensitivity detection capability in complex environments.

The invention relates to the technical field of breast milk oligosaccharide detection, and provides a breast milk oligosaccharide high-coverage analysis method based on liquid chromatography-high resolution mass spectrometry, which comprises the following steps: detecting breast milk oligosaccharide in a to-be-detected sample solution by using a UPLC-HRMS method, comparing fragment ions, retention time, primary parent ion accurate mass number and isotope distribution, and determining the breast milk oligosaccharide coverage degree of the to-be-detected sample solution according to the fragment ions, the retention time, the primary parent ion accurate mass number and the isotope distribution. The structure identification of the breast milk oligosaccharide in the breast milk sample is realized; chromatographic conditions in the UPLC-HRMS method are as follows: a 50mmol / L ammonium formate aqueous solution is taken as a mobile phase A, pure acetonitrile is taken as a mobile phase B, the elution mode is gradient elution, and the content of the mobile phase B is calculated by volume percent. According to the technical scheme, the problems of low coverage and low sensitivity during breast milk oligosaccharide detection and analysis in related technologies are solved.

The invention relates to the technical field of analysis and detection of gas dissolved in oil in an electric power system, and discloses an online monitoring system for gas dissolved in oil and a headspace elution split-flow concentration oil-gas separation method thereof, the system comprises an oil cylinder, a stepping motor, a buffer chamber, a chromatographic analysis module, a standard gas steel cylinder, a heating control module, a valve, a gas pump and a pipeline; wherein the oil cylinder and the stepping motor are horizontally arranged, a lead screw of the stepping motor is connected with a piston in the oil cylinder, and the heating control modules are arranged on the outer sides of the oil cylinder and the chromatographic analysis module; the invention also provides a headspace elution split-flow concentration oil-gas separation method of the online monitoring system for dissolved gas in oil, the headspace elution split-flow concentration oil-gas separation does not need to complete rapid headspace degassing under an ultimate vacuum condition, the degassing time is effectively shortened, and the method is similar to a conventional offline degassing method, so that the cost is reduced. The influence of the vacuum degree and the vacuum degassing rate on an analysis result does not need to be considered, and the device has the advantages of simple structure and good repeatability.

The invention discloses a method for determining anthracene and phenanthrene oxides and polycyclic aromatic hydrocarbon pollutants in tea leaves, and belongs to the technical field of tea leaf component determination, by using the method, whether a tea leaf sample contains anthraquinone, phenanthrenequinone, multiple polycyclic aromatic hydrocarbons and other pollutants can be accurately determined at the same time, and quantitative analysis can be performed. The method has the characteristics of high detection efficiency, accurate analysis result and good repeatability; comprising the following steps: S1, respectively taking reference substances to prepare reference substance stock solutions with different numbers, taking a blank tea reference substance, and carrying out extraction, magnetic separation, extraction and elution to obtain a blank matrix extracting solution; s2, taking each reference substance stock solution, respectively preparing reference substance solutions with different numbers by using a blank matrix extracting solution, and sequentially putting the reference substance solutions into a gas chromatograph-tandem time-of-flight mass spectrometer for determination to obtain spectrum information of each reference substance; and S3, collecting data information of each reference substance from the map information of each reference substance, and then establishing a comparison database.

This invention discloses a method and application for extracting soluble collagen from bones unearthed from archaeological sites, belonging to the field of archaeological sample processing technology. Specifically: 1) After pretreatment, the blocky ancient bone sample is dried at low temperature and ground into bone powder; the bone powder is added to an acidic solution for ultrasonic treatment, centrifuged, and the supernatant is filtered to obtain the sample to be loaded onto a column; 2) The sample to be loaded onto the column is subjected to chromatographic separation, using a gradient elution with equilibration buffer and elution buffer, and a UV detector is used to monitor protein peaks, collecting the target protein solution that elutes within 20-30 minutes; 3) Finally, the target protein solution is concentrated and purified using an ultrafiltration tube to obtain soluble collagen. This invention can obtain a sufficient amount of collagen from ancient deteriorated bone samples, significantly improving sample utilization and providing reliable technical support for bioarchaeological research.

Disclosed is a method for filtering a fibrinogen composition, including the following steps: a) purifying the fibrinogen composition by chromatographic purification using an elution buffer comprising arginine; b) optionally, at least one step of filtering the fibrinogen composition obtained by chromatographic elution in step a), on a filter having a pore size of between 0.08 μm and 0.22 μm, c) filtering the fibrinogen composition obtained by chromatographic elution in step a), or optionally obtained in step b), on a symmetrical filter having a pore size of between 15 nm and 25 nm, and preferably between 18 nm and 22 nm, and d) recovering the resulting fibrinogen solution, the filtering method being carried out without adding arginine after step a), at a high capacity and without a prior freezing and / or thawing step.

The invention relates to the technical field of floridoside and an isomer monomer thereof, and discloses a method for preparing high-purity floridoside and an isomer monomer thereof on a large scale, which is used for carrying out large-scale preparation and purification research on the high-purity floridoside and the isomer monomer thereof and preparing the floridoside and the isomer monomer thereof from a porphyra haitanensis crude extract. And the structure and purity of the compound are identified. The method comprises the following steps: firstly, by utilizing a DART-MS technology, through a single factor experiment, optimizing experiment parameters such as extraction times and elution, and obtaining optimal extraction efficiency; and secondly, based on a two-step purification method of a prep-HPLC (Prep-High Performance Liquid Chromatography) technology, enriching the target compound by using a reversed-phase (C18) mode stationary phase in the first step, and separating the pair of isomers by using a normal-phase (NH3) mode stationary phase in the second step. The obtained floridoside and isomer monomers thereof are subjected to structure and purity identification by mass spectrometry (MS) and nuclear magnetism (NMR). Results are as follows: the two compounds are identified as floridoside and isomers thereof through high-resolution mass spectrometry, and the purities of the floridoside and the isomers thereof are up to 99.5% or above.

The present disclosure is directed to methods of performing affinity chromatography. The injection elution methods disclosed herein afford rapid and robust elution of a target analyte from an affinity chromatography column, therefore improving workflow efficiency. Further, the single injection elution methods result in highly concentrated samples, mitigating the need for sample concentration or manipulation.