539 results about "Hybridoma cell" patented technology

The invention discloses a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof, and relates to the field of genetic engineering, in particular to a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof. The preservation number of the hybridoma cell strain P27-A1 is CGMCC (China General Microbiological Culture Collection Center) No. 46352. The hybridoma cell strain P27-A1 is prepared from feline leukemia virus p27, and a monoclonal antibody secreted by the hybridoma cell strain P27-A1 recognizes a linear epitope antigen of feline leukemia virus p27 protein; the kit for the feline leukemia virus p27 comprises the monoclonal antibody A1 secreted by the hybridoma cell strain P27-A1. The monoclonal antibody secreted by the hybridoma cell strain can greatly improve the sensitivity of the p27 protein for detecting feline leukemia virus. The invention provides the monoclonal antibody secreted by the hybridoma cell strain for identification, and the monoclonal antibody can be used for preparing a diagnostic kit such as a colloidal gold test strip.

The invention belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain and a monoclonal antibody for detecting or identifying MDV-1 and application of the hybridoma cell strain and the monoclonal antibody. The hybridoma cell strain MDV-1Mab-pp38-E2 is obtained through screening by taking pp38 protein of MDV-1 as immunogen, the preservation number is CCTCC NO: C202501, the monoclonal antibody generated by the hybridoma cell strain can recognize I-type chicken Marek's disease virus strains with different serum, and the monoclonal antibody can be used for identifying the type I chicken Marek's disease virus strains with different serum types. The kit has good specificity and sensitivity and can be used for exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines without cross reaction with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common poultry disease viruses, and the kit has good specificity and sensitivity and can be applied to exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines. The kit can also be used for clinical identification, virus content determination and epidemiological investigation of serotype I chicken Marek's disease virus.

The invention relates to the technical field of biological medicine, in particular to a monoclonal antibody of BRVA virus VP7 protein and an epitope and application of the monoclonal antibody. The invention provides a hybridoma cell strain (preservation number: CCTCC NO: C202570). A monoclonal antibody prepared from the hybridoma cell strain can be combined with VP7 protein of G6, G8 and G10 type BRVA; the antigen epitope amino acid sequence of the monoclonal antibody is SEQ ID NO: 1. The monoclonal antibody and the antigen epitope peptide provided by the invention have broad-spectrum neutralizing activity, and provide a new strategy and tool for prevention and control of BRVA.

A specific protein fragment capable of representing salmonella as an antigen is obtained through screening and experimental verification, a mouse is immunized with the specific protein fragment as the antigen, and hybridoma cells capable of stably secreting monoclonal antibodies are obtained through a cell fusion technology. And finally, the anti-salmonella monoclonal antibody with high titer and good specificity is prepared. Similarly, the specific protein fragment is used for immunizing a rabbit to obtain the anti-salmonella polyclonal antibody. On the basis of a mouse monoclonal antibody and a rabbit polyclonal antibody, a salmonella colloidal gold immunochromatography detection method is established, the detection method is good in specificity, and a rapid, simple and convenient detection means is provided for detection of salmonella in food.

The invention discloses a monoclonal antibody targeting all CD45 subtypes and application of the monoclonal antibody, and belongs to the field of monoclonal antibody preparation. The hybridoma cell strain capable of stably secreting all anti-human CD45 subtype antibodies is successfully obtained by stably expressing short and long molecular subtypes of human CD45 extracellular regions in L929 mouse fibroblasts, immunizing BALB / c mice with L929 cells expressing the two molecular subtypes of human CD45, and fusing splenocytes with SP20 myeloma cells after the serum titer reaches the standard. And the monoclonal antibody targeting all subtypes of CD45 is obtained. The monoclonal antibody provided by the invention shows a high-affinity binding characteristic with all human CD45 molecular subtypes, has important application value in the aspect of human CD45 molecular detection, and can be used as a therapeutic antibody in the fields of tumor immunotherapy, autoimmune disease treatment, transplant rejection resistance and the like.

The invention relates to a hybridoma cell strain capable of secreting a napropamide-resistant monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is October 31, 2024, and the preservation number is CGMCC No.46242. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody has the advantages that the hybridoma cell strain Tau4 secreting the napropamide The napropamide-resistant monoclonal antibody secreted by the cell strain is used for analyzing and detecting napropamide residues in food safety detection. The napropamide-resistant monoclonal antibody cell strain obtained by the invention can be used for immunoassay detection, and has better detection sensitivity and specificity (IC50 value is 1.13 ng / mL) on napropamide. The result of the invention can be used for preparing an immunodetection kit and a colloidal gold test strip of napropamide, and provides a powerful detection method and means for detecting the residual quantity of napropamide in foods such as fruits, vegetables and the like.

The invention relates to the technical field of biology, in particular to an anti-B7-H3 monoclonal antibody, a hybridoma cell strain and application thereof. The invention provides a monoclonal antibody. Amino acid sequences of three CDR regions of a heavy chain of the monoclonal antibody respectively have amino acid sequences shown as SEQ ID NO: 11, 12 and 13; the amino acid sequences of the three CDR regions of the light chain of the gene respectively have the amino acid sequences shown as SEQ ID NO: 6, 7 and 8. The anti-B7-H3 monoclonal antibody provided by the invention can be used for specifically recognizing a B7-H3 recombinant protein expressed by eukaryotic expression and a B7-H3 protein expressed on a cell membrane. A B7-H3 detection kit constructed by the anti-B7-H3 monoclonal antibody is high in sensitivity and good in specificity, and has a better application effect in various detections including enzyme-linked immunosorbent assay, flow cytometry, immunofluorescence and the like.

The invention relates to a hybridoma cell strain capable of secreting a mefos monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. According to the invention, the hybridoma cell strain capable of secreting the fenofos monoclonal antibody is obtained through screening, the fenofos monoclonal antibody has high specificity and high sensitivity to the fenofos, IC50 to the fenofos is 0.231 ng / mL, and the fenofos monoclonal antibody has no cross reaction to structural analogues of the fenofos, such as triazophos, diazinon, phorate, acetyl phorate, methamidophos and the like; therefore, low-concentration isoprophos can be accurately detected.

The invention provides an anti-human BCMA monoclonal antibody and a hybridoma cell strain, the antibody has strong affinity with a BCMA antigen, the Kd value is 1.892 * 10 <-8 > M, and the antibody can compete with a commodity BCMA antibody 19F2 to combine with a target cell; the antibody can be specifically combined with a BCMA positive cell line H929, and has no cross reaction with a BCMA negative cell line Raji and peripheral blood source T cells; the monoclonal antibody 2D2 not only can be used for detecting cells expressing human BCMA, but also can be applied to tumor immunotherapy of targeted human BCMA, and has diagnosis and treatment values.

The invention relates to the technical field of biological medicine, in particular to a monoclonal antibody of bovine coronavirus S2 protein, an epitope of the monoclonal antibody and application of the epitope. The invention provides a hybridoma cell strain (preservation number: CCTCC (China Center For Type Culture Collection) NO: C202510). The cell strain can secrete a monoclonal antibody which is targeted and combined with bovine coronavirus S2 protein; the amino acid sequence of the epitope identified by the monoclonal antibody is shown as SEQ ID NO: 1. Experiments prove that the monoclonal antibody has neutralizing activity, and the targeting epitope of the monoclonal antibody is a key neutralizing epitope. The invention provides a new strategy and tool for antibody drug and vaccine research and development as well as diagnosis and detection of the bovine coronavirus.

The invention relates to a hybridoma cell strain capable of secreting dextromethorphan monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain disclosed by the invention has good sensitivity and specificity on dextromethorphan, wherein the IC50 value on the dextromethorphan is 0.162 ng / mL. The monoclonal antibody provided by the invention has no cross reaction on structural analogues of dextromethorphan, such as fentanyl, thilazine, papaverine, morphine and medetomidine, and has good specificity, so that the dextromethorphan with low concentration can be accurately detected.

The invention discloses a pertussis recombinant protein and a monoclonal antibody and application thereof.The pertussis recombinant protein is synthesized by selecting an S1 subunit of pertussis PT and a part of sequences of FHA protein, the pertussis recombinant protein is expressed through a prokaryotic expression system and purified, the protein serves as an antigen to immunize a mouse, and the pertussis recombinant protein is obtained. Positive hybridoma cell strains 3B10, 3D9 and 5F7 capable of stably secreting the antibody are obtained by adopting a hybridoma technology, the preservation numbers are respectively CCTCC NO: C202567, CCTCC NO: C202568 and CCTCC NO: C202569, and the obtained monoclonal antibody has relatively high affinity and detection sensitivity to pertussis PT and FHA recombinant proteins, is used for detecting a bordetella pertussis antigen, is strong in specificity and high in sensitivity, can be used for instant detection, and can be applied to clinical application. And the method has a relatively high application value.

The invention discloses a herbicide-resistant protein GAT hybridoma cell strain, an antibody generated by the herbicide-resistant protein GAT hybridoma cell strain and an application of the herbicide-resistant protein GAT hybridoma cell strain. The herbicide-resistant protein GAT hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46333. The preparation method comprises the following steps: a) purifying prokaryotic expression to obtain GAT recombinant protein; b) immunizing animals: immunizing BALB / c mice by taking the GAT recombinant protein as an antigen; c) cell fusion: splenocytes of the immune BALB / c mice are collected and fused with SP2 / 0 cells; and d) cell strain establishment: carrying out subcloning through a limited dilution method, carrying out ELISA detection after 5-7 days of subcloning until a hybridoma cell strain capable of stably secreting a positive antibody is screened out, and carrying out amplification, re-culture and storage. The monoclonal antibody secreted by the hybridoma cell strain lays a foundation for detection of herbicide-resistant protein GAT in transgenic crops, so that development, seed production and seed conservation of transgenic gat crops are supported, and stable industrial promotion of the transgenic gat crops and sustainable application of related transformants are guaranteed.

The invention belongs to the technical field of immunology, and particularly relates to an anti-mandarin fish immune globulin D (IgD) monoclonal antibody as well as a preparation method and application thereof. The invention provides a hybridoma cell strain 5C7 capable of secreting an anti-fish IgD monoclonal antibody or an antigen binding fragment thereof for the first time, the hybridoma cell strain 5C7 is preserved in China Center for Type Culture Collection on January 16, 2025, and the preservation number is CCTCC NO: C202550. The anti-mandarin fish IgD monoclonal antibody or the antigen binding fragment thereof can be subjected to specific reaction with mandarin fish secreting type IgD protein and B cell surface film type IgD, can be applied to detection of IgD in a mandarin fish serum sample, and can also be applied to detection of IgD + B cell surface markers.

The invention discloses a porcine reproductive and respiratory syndrome virus M protein antigen epitope peptide and a monoclonal antibody thereof. The amino acid sequence of the antigen epitope peptide is as shown in SEQ ID No. 11; the heavy chain variable region of the monoclonal antibody comprises CDR1-3 as shown in SEQ ID No.1 to SEQ ID No.3; the light chain variable region comprises CDR1-3 as shown in SEQ ID No. 4 to 6; the monoclonal antibody reacts with a plurality of PRRSV strains such as HP-PRRSV, NADC30PRRSV, NADC34PRRSV and the like, and the conservative property is good; the antibody can be obtained by biological means such as conventional genetic engineering, and the problem that the genetic sequence of the antibody is easy to lose due to long-term cryopreservation of traditional hybridoma cells is avoided; optimization of the antibody on the gene or protein level is facilitated, and the specificity and affinity of the antibody are further improved.

The invention discloses a monoclonal antibody, a hybridoma cell secreting the monoclonal antibody and application of the hybridoma cell, and belongs to the field of peptides. The invention aims to provide a monoclonal antibody and a hybridoma cell secreting the monoclonal antibody. The hybridoma cell secreting the monoclonal antibody disclosed by the invention is a hybridoma cell 3G3, and the preservation number of the hybridoma cell 3G3 in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.46345. The monoclonal antibody and the BVDV antibody detection kit based on the monoclonal antibody and the blocking ELISA method can be used for detecting a neutralizing antibody of the BVDV, and have the characteristics of strong specificity, good sensitivity, high sensitivity, high accuracy and the like.

The invention relates to a medetomidine hapten, a monoclonal antibody, a hybridoma cell strain and application thereof, and belongs to the technical field of immunodetection. A medetomidine complete antigen is obtained by synthesizing a medetomidine hapten, a mouse is used as an immune animal to prepare a hybridoma cell strain secreting a medetomidine monoclonal antibody, the hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.46518. The medetomidine monoclonal antibody secreted by the hybridoma cell strain has better detection sensitivity and detection specificity to medetomidine, the IC50 value is 10.42 ng / mL, and the cross reaction rate to various medetomidine analogues is lt; the method can be applied to preparation of medetomidine detection products, and provides a reliable tool for immunodetection of medetomidine.

Use of impedance and / or dielectric spectroscopy for analysis of a eukaryotic cell expressing an endogenous or recombinant peptide or polypeptide. A sensing apparatus 30 includes a main channel 34 thro

The invention provides an anti-duck adenovirus 3 type monoclonal antibody, a hybridoma cell strain and application thereof, the anti-duck adenovirus 3 type monoclonal antibody is secreted by a hybridoma cell strain 2F12, the classification name of the hybridoma cell strain 2F12 is Hybridoma cell line 2F12, the hybridoma cell strain 2F12 is preserved in China Center for Type Culture Collection on November 13, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CGMCC NO. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: The blocking ELISA kit for detecting the duck adenovirus 3 antibody, which is prepared by utilizing the monoclonal antibody, is strong in specificity, high in sensitivity and good in repeatability, and has a very good application prospect in the aspects of epidemiological investigation of the duck adenovirus 3, vaccine immune efficacy evaluation and diagnosis of the duck adenovirus 3 disease.

An anti-BDCA-2 antibody, a preparation method therefor, and a use thereof, relating to the field of antibodies. The preparation method for the anti-BDCA-2 antibody comprises: carrying out ELISA detection and screening on a hybridoma cell culture supernatant and an antigen protein to obtain a positive clone, and subcloning the positive clone to obtain the anti-BDCA-2 antibody. The anti-BDCA-2 antibody comprises a heavy chain and a light chain, wherein a variable region of the heavy chain and a variable region of the light chain are selected from amino acid sequences as shown in SEQ ID NOs: 1-22. The anti-BDCA-2 antibody and related drugs can be used for inhibiting the production of IFN-α by pDCs and preventing the over-activation of the pDCs. The anti-BDCA-2 antibody can effectively inhibit the activation of pDC cells and the production of IFN-α, and has great potential in resisting virus infection and preventing certain autoimmune diseases.

The invention discloses an anti-streptomycin monoclonal antibody and application thereof, and relates to the technical field of streptomycin detection. According to the invention, a hybridoma cell strain capable of stably secreting an anti-streptomycin monoclonal antibody is screened, and the preservation number of the hybridoma cell strain is CGMCC (China General Microbiological Culture Collection Center And screening to obtain the specific and sensitive streptomycin monoclonal antibody. Immunodetection experiments prove that the streptomycin monoclonal antibody provided by the invention has high specificity to streptomycin, high detection sensitivity to streptomycin and wide linear detection range. Therefore, the anti-streptomycin monoclonal antibody provided by the invention can be used for developing streptomycin detection kits, test strips and other products, and a detection tool and means are provided for rapid detection of streptomycin and other antibiotic residues in animal products, aquatic products and agriculture.

The invention provides an anti-BDCA-2 antibody as well as a preparation method and application thereof, and belongs to the field of antibodies. The invention provides a preparation method of an anti-BDCA-2 antibody, which comprises the following steps: carrying out ELISA detection screening on a hybridoma cell culture supernatant and an antigen protein to obtain a positive clone, and carrying out subcloning on the positive clone to obtain the anti-BDCA-2 antibody. The anti-BDCA-2 antibody comprises a heavy chain and a light chain, and a variable region of the heavy chain and a variable region of the light chain are selected from amino acid sequences as shown in SEQ ID NO: 1-22. The anti-BDCA-2 antibody and related drugs provided by the invention can be used for inhibiting generation of IFN-alpha by pDC and preventing excessive activation of pDC. The anti-BDCA-2 antibody produced by the invention can effectively inhibit the activation of pDC cells and the generation of IFN-alpha, and has great potential in resisting virus infection and preventing some autoimmune diseases.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain capable of secreting a clostridium perfringens ETX monoclonal antibody with neutralizing activity and application of the hybridoma cell strain. On the basis of natural toxin, an indirect ELISA (enzyme-linked immunosorbent assay) detection method is established by utilizing a non-toxic ETX single amino acid site protein mutant, and a series of hybridoma cell strains secreting an anti-clostridium perfringens ETX monoclonal antibody are screened; the hybridoma cell strain can stably and efficiently secrete a monoclonal antibody with neutralizing activity and a monoclonal antibody without neutralizing resistance respectively, and large-scale batch production can be realized. According to the present invention, the clostridium perfringens ETX monoclonal antibody is firstly utilized to establish the ETX antigen ELISA detection method, and the method has characteristics of simple sample operation, low cost, rapid reaction, strong specificity and the like, can provide the reference for the diagnosis of clostridium perfringens, and provides the basis for the research of the related vaccine potency test substitution method.

The invention relates to the technical field of biology, in particular to an anti-porcine CD82 protein monoclonal antibody, a hybridoma cell strain and application, the monoclonal antibody is prepared by the following steps: expressing and purifying a CD82 (EC2) region by utilizing a prokaryotic expression system, preparing by taking the purified recombinant protein as an immunogen, and carrying out indirect ELISA (enzyme-linked immuno sorbent assay) and Flow Cytometry screening to obtain the monoclonal cell strain capable of secreting an IgG2a type antibody. The purified antibody is detected through an indirect ELISA method, sensitive reaction still exists when the antibody concentration reaches 31.25 ng / ml, PAM cells capable of expressing endogenous CD82 protein are detected through flow cytometry, and the result shows that the mAb has good marking capacity for the PAM cells, indicating that the mAb of the CD82 protein capable of being used for flow cytometry is successfully prepared. A biological tool is provided for further analyzing the function and mechanism of the CD82 protein.

The application discloses a hybridoma cell strain secreting an anti-tilapia interleukin-2 monoclonal antibody and an application thereof, and the hybridoma cell strain 1C11F5 is preserved in the China Center for Type Culture Collection (CCTCC) on March 9, 2024, and the preservation number is CCTCC NO: C202470. The application can specifically identify the tilapia IL-2 protein and the lymphocyte group secreting IL-2, is helpful for further exploration of T cell activation and proliferation, and provides an important immunological tool for adaptive immune mechanism research of hard fish (such as tilapia and the like).

The application discloses a hybridoma cell strain 6F92E10, a monoclonal antibody thereof and application thereof, and belongs to the technical field of biotechnology.The application provides a hybridoma cell strain secreting an anti-mucinophilic Akkermansia monoclonal antibody.The antibody secreted by the hybridoma cell strain only combines with AKKermansia and does not cross-react with other common intestinal bacteria.In addition, the application solves how to detect the adhesion and colonization of mucinophilic Akkermansia in the colon of a mouse by using the prepared monoclonal antibody through an indirect immunofluorescence localization experiment, and expands the application range of the antibody.

The present application relates to a hybridoma cell strain secreting psoralen and isopsoralen monoclonal antibody. The hybridoma cell strain of the present application is preserved in China General Microbiological Culture Collection Center on April 18, 2024, the preservation address is No. 1, Xibaixili, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 45909. The psoralen and isopsoralen monoclonal antibody secreted by the hybridoma cell strain of the present application has good sensitivity and specificity to psoralen and isopsoralen, so that the psoralen and isopsoralen can be efficiently and rapidly detected.

This invention discloses an antibody that specifically recognizes Toxoplasma gondii surface antigens and its applications. The invention utilizes hybridoma technology to establish a hybridoma cell line by fusing Balb / C mouse spleen cells with SP2 / 0 cells. Monoclonal antibodies were prepared from these cells using in vitro culture and in vivo ascites induction methods, yielding the monoclonal antibody SH12-S7. This invention provides clinical and research institutions with an antibody that specifically recognizes Toxoplasma gondii surface antigens. It can be used for ELISA assays to detect clinical samples, for immunohistochemical assays to diagnose pathological specimens, and provides a convenient research tool for toxoplasmosis research departments. It has broad application value in the diagnosis and detection of definitive host infections and provides technical support for epidemiological investigations and evaluation of control effects of Toxoplasma gondii.

The present invention provides a novel anti-Aβ antibody capable of detecting an Aβ related peptide, a method for measuring an Aβ related peptide in a biological sample by immunochemically using the above-mentioned novel anti-Aβ antibody, and a kit. A monoclonal antibody that recognizes an Aβ related peptide, and is produced by a hybridoma deposited under the accession number NITE BP-02998 at the NITE Patent Microorganisms Depositary of the National Institute of Technology and Evaluation. An antibody-immobilized carrier that includes a carrier and the monoclonal antibody defined in claim 1 bound to the carrier. A kit for measuring an Aβ related peptide, comprising the antibody-immobilized carrier.

The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.