886 results about "Hybridoma cell" patented technology

The invention relates to a mouse anti-PBP2a hybridoma cell strain, a monoclonal antibody and application of the mouse anti-PBP2a hybridoma cell strain, staphylococcus aureus PBP2a protein is extracted as an antigen, after the high-purity antigen is obtained, a good immunoreaction is aroused in a mouse body, then a hybridoma technology is adopted, two cell strains with high affinity and specificity are screened, and the corresponding antibody is prepared; the two antibodies are used as a gold-labeled antibody and a coating antibody in a colloidal gold immunoassay kit, and the kit can be used for detecting PBP2a protein in methicillin-resistant staphylococcus aureus and can be used for developing related in-vitro diagnostic reagents.

The invention relates to the technical field of biomedicine, in particular to an anti-influenza A virus NP protein antibody pair and application thereof. The antibody pair comprises two monoclonal antibodies; the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; one monoclonal antibody in the monoclonal antibody pair is named as a 10G10 antibody, and the other monoclonal antibody is named as a 11F8 antibody. The invention also discloses a nucleic acid molecule for coding the monoclonal antibody and a hybridoma cell strain for secreting the monoclonal antibody. In addition, the invention also discloses a kit for detecting influenza virus protein, which comprises the monoclonal antibody pair, the 10G10 antibody is used as a capture antibody, and the 11F8 antibody is used as a labeled antibody. The antibody for resisting the influenza A virus NP protein provided by the invention has relatively good specificity on the influenza virus protein.

The invention belongs to the field of monoclonal antibodies, and particularly relates to a mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody, a hybridoma cell strain secreting the monoclonal antibody and application, a CDR region of a heavy chain variable region of the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody comprises three sequences shown in SEQ ID NO.1-3, and a CDR region of a light chain variable region of the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody comprises three sequences shown in SEQ ID NO.4-6; the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody can be obtained by being secreted by a hybridoma cell strain PFM-1B7C12A1B2 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: C2024234. The mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody provided by the invention can be used for Western blot and ELISA specific detection of IgM in a pelteobagrus fulvidraco secretion form, and can be used for flow cytometry identification of pelteobagrus fulvidraco IgM + B cells. The monoclonal antibody is good in specificity, high in sensitivity and strong in affinity, can be used for structural analysis, immune response level detection and the like of pelteobagrus fulvidraco IgM, and lays a foundation for in-depth study of a pelteobagrus fulvidraco immune system and establishment of a vaccine immune effect evaluation method taking the antibody level as an index.

The invention provides an FWCH2 monoclonal antibody and a preparation method and application of a kit of the FWCH2 monoclonal antibody. A monoclonal antibody hybridoma cell strain FWCH2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46112, and the prepared monoclonal antibody can effectively recognize the FWCH2.

The invention discloses a porcine reproductive and respiratory syndrome virus N protein antigen epitope peptide and a monoclonal antibody thereof. The amino acid sequence of the antigen epitope peptide is as shown in SEQ ID No. 11; the heavy chain variable region of the monoclonal antibody comprises CDR1-3 as shown in SEQ ID No.1 to SEQ ID No.3; the light chain variable region comprises CDR1-3 as shown in SEQ ID No. 4 to 6; the monoclonal antibody reacts with a variety of PRRSV strains such as classical PRRSV, HP-PRRSV, NADC30PRRSV-like, NADC34PRRSV-like, European PRRSV and the like, and the conservative property is good; the antibody can be obtained by biological means such as conventional genetic engineering, and the problem that the genetic sequence of the antibody is easy to lose due to long-term cryopreservation of traditional hybridoma cells is avoided; optimization of the antibody on the gene or protein level is facilitated, and the specificity and affinity of the antibody are further improved.

The invention discloses a bovine rotavirus monoclonal antibody and a preparation method of a bovine rotavirus quantum dot immunochromatography test strip, and belongs to the technical field of bovine rotavirus monoclonal antibodies. According to the invention, a hybridoma cell strain BRV 3G9 is utilized, and a monoclonal antibody is prepared by adopting an ascites method; the BRV 3G9 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46138. The BRV 3G9 is named as BRV 3G9. According to the present invention, the concentrated live virus immunized mouse is adopted to prepare the monoclonal antibody with strong immunoreaction, and the BRV quantum dot immunochromatography test strip is researched and developed on the basis of the monoclonal antibody, and has advantages of convenient use, high efficiency, high sensitivity, strong specificity and the like, and can achieve the rapid clinical differential diagnosis of calf diarrhea pathogens, the problem that grassroots veterinarians lack a clinical rapid detection technology is solved, the diagnosis and treatment level and the prevention and treatment effect are improved, and technical guarantee is provided for efficiently preventing and treating calf diarrhea and guaranteeing calf health.

The invention relates to an anti-PRV gD (pseudorabies virus gD) protein neutralizing monoclonal antibody, a hybridoma cell and application of the anti-PRV gD protein neutralizing monoclonal antibody. The anti-PRV gD neutralizing monoclonal antibody is prepared by constructing a PRV gD eukaryotic expression vector, transduction the PRV gD eukaryotic expression vector into a CHO (Chinese hamster ovary) cell for expression and then carrying out animal immunization by taking a PRV gD recombinant protein and an attenuated live vaccine as immunogens. The neutralizing monoclonal antibody or hybridoma cell provided by the invention lays a foundation for the development of a rapid detection technology of the pseudorabies virus and the gD protein thereof, has wide research and application values and commercial use values in immunodetection related to the pseudorabies virus, and has important guiding significance for vaccine development based on the pseudorabies virus.

The invention provides a monoclonal antibody capable of recognizing a recombinant EGF-CRM197 vaccine and a preparation method thereof, on the basis of researching, developing and preparing the recombinant EGF-CRM197 vaccine, after the recombinant EGF-CRM197 vaccine is further immunized and inoculated to a mouse, mouse spleen cells and mouse myeloma cells are fused, and the monoclonal antibody capable of recognizing the recombinant EGF-CRM197 vaccine is obtained. And screening a hybridoma cell strain which generates a specific monoclonal antibody for recognizing the recombinant EGF-CRM197 vaccine, and sequencing to obtain an antibody sequence. After the hybridoma cells are cultured in a mouse body, the monoclonal antibody for recognizing the recombinant EGF-CRM197 vaccine can be prepared in vitro through methods of chromatographic purification and the like. The method can be used for detecting the release titer of the recombinant EGF-CRM197 vaccine and evaluating the immune efficacy after the recombinant EGF-CRM197 vaccine is immunized.

The invention discloses a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof, and relates to the field of genetic engineering, in particular to a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof. The preservation number of the hybridoma cell strain P27-A1 is CGMCC (China General Microbiological Culture Collection Center) No. 46352. The hybridoma cell strain P27-A1 is prepared from feline leukemia virus p27, and a monoclonal antibody secreted by the hybridoma cell strain P27-A1 recognizes a linear epitope antigen of feline leukemia virus p27 protein; the kit for the feline leukemia virus p27 comprises the monoclonal antibody A1 secreted by the hybridoma cell strain P27-A1. The monoclonal antibody secreted by the hybridoma cell strain can greatly improve the sensitivity of the p27 protein for detecting feline leukemia virus. The invention provides the monoclonal antibody secreted by the hybridoma cell strain for identification, and the monoclonal antibody can be used for preparing a diagnostic kit such as a colloidal gold test strip.

The invention belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain and a monoclonal antibody for detecting or identifying MDV-1 and application of the hybridoma cell strain and the monoclonal antibody. The hybridoma cell strain MDV-1Mab-pp38-E2 is obtained through screening by taking pp38 protein of MDV-1 as immunogen, the preservation number is CCTCC NO: C202501, the monoclonal antibody generated by the hybridoma cell strain can recognize I-type chicken Marek's disease virus strains with different serum, and the monoclonal antibody can be used for identifying the type I chicken Marek's disease virus strains with different serum types. The kit has good specificity and sensitivity and can be used for exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines without cross reaction with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common poultry disease viruses, and the kit has good specificity and sensitivity and can be applied to exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines. The kit can also be used for clinical identification, virus content determination and epidemiological investigation of serotype I chicken Marek's disease virus.

The invention provides a K1143 monoclonal antibody and a preparation method and application of a kit of the K1143 monoclonal antibody. The monoclonal antibody hybridoma cell strain K1143 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46110, and the prepared monoclonal antibody can effectively recognize K1143. The invention further discloses a preparation method of the monoclonal antibody K1143.

The invention provides a ZN428 monoclonal antibody and a preparation method and application of a kit of the ZN428 monoclonal antibody. The monoclonal antibody hybridoma cell strain ZN428 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46111, and the prepared monoclonal antibody can effectively recognize the ZN428.

The invention relates to the technical field of biological medicine, in particular to a monoclonal antibody of BRVA virus VP7 protein and an epitope and application of the monoclonal antibody. The invention provides a hybridoma cell strain (preservation number: CCTCC NO: C202570). A monoclonal antibody prepared from the hybridoma cell strain can be combined with VP7 protein of G6, G8 and G10 type BRVA; the antigen epitope amino acid sequence of the monoclonal antibody is SEQ ID NO: 1. The monoclonal antibody and the antigen epitope peptide provided by the invention have broad-spectrum neutralizing activity, and provide a new strategy and tool for prevention and control of BRVA.

The invention discloses preparation and application of an antibody capable of detecting various IMP variants, and belongs to the field of biological medicine. According to the invention, a conserved epitope sequence of IMP enzyme is obtained according to comparison of amino acid sequences of all IMP variants, and an antibody of a spatial epitope is prepared through structural analysis of the epitope. After a mouse is immunized with IMP immunogen protein, hybridoma cells secreting antibodies are obtained through multi-technology platform screening, specific antibody pairs MK35G9 and MK56G2 which are high in affinity and can be combined with multiple IMP variants are obtained after sequencing, and corresponding CDR is obtained through analysis. According to the present invention, the IMP enzyme detection product development is performed based on the immunochromatography platform, such that advantages of high sensitivity, good specificity, covering of a variety of IMP variants, and rapid and convenient detection are provided, and important help is provided for clinical auxiliary diagnosis and medication guidance.

The invention provides an SCGB1D4 monoclonal antibody and a preparation method and application of a kit of the SCGB1D4 monoclonal antibody. An applicant is preserved in the China General Microbiological Culture Collection Center (CGMCC) through a hybridoma cell strain SCGB1D4, and the preservation number is CGMCC No.45958. The prepared monoclonal antibody can effectively recognize the SCGB1D4.

The invention belongs to the field of biology, and discloses a monoclonal antibody of avian influenza virus PB2 protein, the monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and can specifically bind to influenza virus matrix protein PB2, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO.1; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO. 2; the hybridoma cell has relatively high sensitivity and specificity, and can be specifically combined with at least five subtype avian influenza PB2 proteins; meanwhile, the invention also provides application and drugs of the labeled compound, the hybridoma cell and the monoclonal antibody.

The invention discloses an anti-HCV (hepatitis C virus) core antigen monoclonal antibody, a hybridoma cell strain and application, and particularly relates to the hybridoma cell strain screened according to a common conserved sequence of core proteins of different genotypes of HCV, the antibody secreted by the hybridoma cell strain is applied to HCV antigen detection, different genotypes of hepatitis C viruses can be detected, and the monoclonal antibody has good inclusiveness and can be applied to detection of HCV antigens. The technical effect can be realized by only two antibodies, so that the production cost of the HCV antigen detection kit can be reduced, the production difficulty can be reduced, and the production efficiency can be improved.

A specific protein fragment capable of representing salmonella as an antigen is obtained through screening and experimental verification, a mouse is immunized with the specific protein fragment as the antigen, and hybridoma cells capable of stably secreting monoclonal antibodies are obtained through a cell fusion technology. And finally, the anti-salmonella monoclonal antibody with high titer and good specificity is prepared. Similarly, the specific protein fragment is used for immunizing a rabbit to obtain the anti-salmonella polyclonal antibody. On the basis of a mouse monoclonal antibody and a rabbit polyclonal antibody, a salmonella colloidal gold immunochromatography detection method is established, the detection method is good in specificity, and a rapid, simple and convenient detection means is provided for detection of salmonella in food.

The invention discloses a monoclonal antibody targeting all CD45 subtypes and application of the monoclonal antibody, and belongs to the field of monoclonal antibody preparation. The hybridoma cell strain capable of stably secreting all anti-human CD45 subtype antibodies is successfully obtained by stably expressing short and long molecular subtypes of human CD45 extracellular regions in L929 mouse fibroblasts, immunizing BALB / c mice with L929 cells expressing the two molecular subtypes of human CD45, and fusing splenocytes with SP20 myeloma cells after the serum titer reaches the standard. And the monoclonal antibody targeting all subtypes of CD45 is obtained. The monoclonal antibody provided by the invention shows a high-affinity binding characteristic with all human CD45 molecular subtypes, has important application value in the aspect of human CD45 molecular detection, and can be used as a therapeutic antibody in the fields of tumor immunotherapy, autoimmune disease treatment, transplant rejection resistance and the like.

The invention discloses a cell strain capable of generating antibodies for detecting Pseudomonas amygdali and diseases caused by the Pseudomonas amygdali and application of the cell strain. The preservation number of the cell strain is CCTCC (China Center For Type Culture Collection) NO: C2024169 and / or CCTCC NO: C2024170. The monoclonal antibody generated by the monoclonal antibody hybridoma cell strain can be prepared into handheld colloidal gold rapid test paper, and the pseudomonas amygdali in a sample can be rapidly and sensitively detected on site.

The invention provides an R3HD4 monoclonal antibody and a preparation method and application of a kit of the R3HD4 monoclonal antibody. The monoclonal antibody hybridoma cell strain R3HD4 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46114, and the prepared monoclonal antibody can effectively recognize R3HD4. The invention further discloses a preparation method of the monoclonal antibody.

The invention provides a CK052 monoclonal antibody and a preparation method and application of a kit of the CK052 monoclonal antibody. The monoclonal antibody hybridoma cell strain CK052 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46107. The prepared monoclonal antibody and the kit thereof can effectively recognize the CK052.

The invention relates to a hybridoma cell strain capable of secreting a napropamide-resistant monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is October 31, 2024, and the preservation number is CGMCC No.46242. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody has the advantages that the hybridoma cell strain Tau4 secreting the napropamide The napropamide-resistant monoclonal antibody secreted by the cell strain is used for analyzing and detecting napropamide residues in food safety detection. The napropamide-resistant monoclonal antibody cell strain obtained by the invention can be used for immunoassay detection, and has better detection sensitivity and specificity (IC50 value is 1.13 ng / mL) on napropamide. The result of the invention can be used for preparing an immunodetection kit and a colloidal gold test strip of napropamide, and provides a powerful detection method and means for detecting the residual quantity of napropamide in foods such as fruits, vegetables and the like.

The invention relates to the technical field of biology, in particular to an anti-B7-H3 monoclonal antibody, a hybridoma cell strain and application thereof. The invention provides a monoclonal antibody. Amino acid sequences of three CDR regions of a heavy chain of the monoclonal antibody respectively have amino acid sequences shown as SEQ ID NO: 11, 12 and 13; the amino acid sequences of the three CDR regions of the light chain of the gene respectively have the amino acid sequences shown as SEQ ID NO: 6, 7 and 8. The anti-B7-H3 monoclonal antibody provided by the invention can be used for specifically recognizing a B7-H3 recombinant protein expressed by eukaryotic expression and a B7-H3 protein expressed on a cell membrane. A B7-H3 detection kit constructed by the anti-B7-H3 monoclonal antibody is high in sensitivity and good in specificity, and has a better application effect in various detections including enzyme-linked immunosorbent assay, flow cytometry, immunofluorescence and the like.

The invention relates to a hybridoma cell strain capable of secreting a mefos monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. According to the invention, the hybridoma cell strain capable of secreting the fenofos monoclonal antibody is obtained through screening, the fenofos monoclonal antibody has high specificity and high sensitivity to the fenofos, IC50 to the fenofos is 0.231 ng / mL, and the fenofos monoclonal antibody has no cross reaction to structural analogues of the fenofos, such as triazophos, diazinon, phorate, acetyl phorate, methamidophos and the like; therefore, low-concentration isoprophos can be accurately detected.

The invention provides an anti-human BCMA monoclonal antibody and a hybridoma cell strain, the antibody has strong affinity with a BCMA antigen, the Kd value is 1.892 * 10 <-8 > M, and the antibody can compete with a commodity BCMA antibody 19F2 to combine with a target cell; the antibody can be specifically combined with a BCMA positive cell line H929, and has no cross reaction with a BCMA negative cell line Raji and peripheral blood source T cells; the monoclonal antibody 2D2 not only can be used for detecting cells expressing human BCMA, but also can be applied to tumor immunotherapy of targeted human BCMA, and has diagnosis and treatment values.

The invention relates to the technical field of biological medicine, in particular to a monoclonal antibody of bovine coronavirus S2 protein, an epitope of the monoclonal antibody and application of the epitope. The invention provides a hybridoma cell strain (preservation number: CCTCC (China Center For Type Culture Collection) NO: C202510). The cell strain can secrete a monoclonal antibody which is targeted and combined with bovine coronavirus S2 protein; the amino acid sequence of the epitope identified by the monoclonal antibody is shown as SEQ ID NO: 1. Experiments prove that the monoclonal antibody has neutralizing activity, and the targeting epitope of the monoclonal antibody is a key neutralizing epitope. The invention provides a new strategy and tool for antibody drug and vaccine research and development as well as diagnosis and detection of the bovine coronavirus.

The invention provides a TM125 monoclonal antibody and a preparation method and application of a kit of the TM125 monoclonal antibody. The monoclonal antibody hybridoma cell strain TM125 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46109, and the prepared monoclonal antibody can effectively recognize the TM125.

The invention discloses a hybridoma cell strain 1D6 as well as a monoclonal antibody and application thereof. The invention also discloses an application of the monoclonal antibody for resisting the Tilletia foetida in the detection of teliospore of the Tilletia foetida. The invention also discloses a single base sequence DNA single chain modified Tilletia foetida monoclonal antibody. The invention also discloses a detection kit. The monoclonal antibody as well as the marker and the kit thereof show high specific recognition capability on Tilletia foetida. The method for detecting the single base by mass spectrometry has a signal amplification effect and is simple and convenient in detection operation, so that the sensitivity and the detection efficiency of the method are remarkably improved. With the progress and continuous popularization and application of the mass spectrum technology, the technology has wide application potential and prospect.

The invention provides a hybridoma cell strain secreting a clotrimazole monoclonal antibody and application of the hybridoma cell strain, and relates to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to clotrimazole, the IC50 value of clotrimazole is 7.386 ng / mL, and the monoclonal antibody has good sensitivity; moreover, the clotrimazole monoclonal antibody has no cross reaction on functional analogues of clotrimazole, such as miconazole, econazole, ketoconazole, bifonazole and sertaconazole, and has good specificity. Therefore, the clotrimazole monoclonal antibody secreted by the hybridoma cell strain disclosed by the invention can be used for accurately detecting low-concentration clotrimazole.