50 results about "Hybridoma cell" patented technology

The present invention provides a novel anti-Aβ antibody capable of detecting an Aβ related peptide, a method for measuring an Aβ related peptide in a biological sample by immunochemically using the above-mentioned novel anti-Aβ antibody, and a kit. A monoclonal antibody that recognizes an Aβ related peptide, and is produced by a hybridoma deposited under the accession number NITE BP-02998 at the NITE Patent Microorganisms Depositary of the National Institute of Technology and Evaluation. An antibody-immobilized carrier that includes a carrier and the monoclonal antibody defined in claim 1 bound to the carrier. A kit for measuring an Aβ related peptide, comprising the antibody-immobilized carrier.

This invention relates to a hybridoma cell line that secretes a Cy3.5 monoclonal antibody and its application, belonging to the field of immunoassay. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46741. The Cy3.5 monoclonal antibody secreted by this cell line exhibits excellent affinity and sensitivity to Cy3.5, with an IC50 of [missing value]. 50 Reaching 10 ng / mL, the IC50 for Cy5.5 50 An immunoassay product based on this hybridoma cell line, with a concentration of up to 50 ng / mL, can be used for the detection of Cy3.5 and its analogues, providing an efficient detection method and means for the residual detection of Cy3.5.

This invention provides an anti-CD16 antibody, the amino acid sequence of which is selected from SEQ ID NO. 1-3 in the heavy chain CDR region and from SEQ ID NO. 4-6 in the light chain CDR region. This invention belongs to the field of antibodies. It provides a protein sequence of an anti-human CD16 mouse monoclonal antibody, and designs a gene expression base sequence based on the sequenced protein sequence. A stable CHO cell line for recombinant expression of this gene is established. The purified antibody can specifically recognize the human CD16 antigen, and the antibody expression yield is much higher than that of antibodies prepared using traditional hybridoma cells. Furthermore, by replacing mouse IgG1 with rabbit IgG while retaining the variable region, a stable CHO cell line for recombinant expression of the rabbit monoclonal antibody is established. The purified antibody maintains its specific recognition of the human CD16 antigen.

This invention relates to a hybridoma cell line secreting a monoclonal antibody against pendimethalin and its application, belonging to the field of immunoassay. The hybridoma cell line secreting the pendimethalin monoclonal antibody of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46744. The pendimethalin monoclonal antibody secreted by this hybridoma cell line is used for the analysis and detection of pendimethalin residues in food safety testing. The pendimethalin monoclonal antibody cell line obtained by this invention can be used for immunoassay detection, exhibiting good detection sensitivity and specificity (IC50) for pendimethalin. 50 The value was 1.87 ng / mL.

This invention belongs to the field of biotechnology, providing a mouse anti-human ELL2 monoclonal antibody, its preparation method, and its applications. This invention successfully obtained a 2E1 anti-human ELL2 monoclonal antibody and established a hybridoma cell line capable of stably producing this antibody. The binding affinity (KD) of the obtained 2E1 monoclonal antibody to human ELL2 protein reached the picomolar level (approximately 10^-3 kDa). ‑12 The antibody (M) exhibits extremely strong binding ability. Cross-reactivity tests confirmed that it binds only to human ELL2, and shows no binding signal with highly homologous human ELL1, ELL3, and mouse ELL2, thus completely solving the specificity problem of existing technologies.

The present application relates to a hybridoma cell strain secreting a chlorinated nitrogen aminophenazone monoclonal antibody and its application, and belongs to the technical field of immune detection. The hybridoma cell strain secreting a chlorinated nitrogen aminophenazone monoclonal antibody was preserved in the China General Microbiological Culture Collection Center on April 24, 2026, and the address of the preservation is No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 46821. The monoclonal antibody secreted by the hybridoma cell strain of the present application has good detection sensitivity (IC 50 Value is 0.27 ng / mL) to chlorinated nitrogen aminophenazone, and can be used for the residual detection of chlorinated nitrogen aminophenazone.

This invention relates to a hybridoma cell line that secretes a monoclonal antibody against azoxystrobin and its application, belonging to the field of immunochemistry. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46742, and classified as a monoclonal cell line. The monoclonal antibody secreted by the invented hybridoma cell line exhibits good detection sensitivity (IC50) against azoxystrobin. 50 With a value of 0.049 ng / mL, it can be used for the detection of antimicrobial residues.

The application provides an abnormal prothrombin detection kit and application thereof, and relates to the technical field of in-vitro detection.The application provides a kit for abnormal prothrombin detection, which comprises an abnormal prothrombin antibody, wherein the abnormal prothrombin antibody is derived from a hybridoma cell strain 7E8-4-4-1 of fusion of myeloma cells and mouse B cells, and the hybridoma cell strain 7E8-4-4-1 is preserved in the China General Microbiological Culture Collection Center on October 28, 2025, with a preservation number of CGMCC No.46709.The kit has a large detection range, a small lower limit of detection and high accuracy, and has great application potential in the detection of abnormal prothrombin.

This invention relates to a hybridoma cell line that secretes a monoclonal antibody against Basic Yellow O and its application, belonging to the field of immunoassay. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46740. This invention obtains the Basic Yellow O hapten through multiple screenings and experiments, then prepares the Basic Yellow O complete antigen from the hapten, and uses this complete antigen to immunize animals to obtain a hybridoma cell line. The Basic Yellow O monoclonal antibody secreted by this cell line exhibits excellent affinity and sensitivity to Basic Yellow O, with an IC50 value of [missing information - likely a value]. 50 With a concentration of 1 ng / mL, it can be used to prepare immunoassay products for Basic Yellow O, providing an efficient detection method and means for the detection of Basic Yellow O residues in food.

This invention relates to a hybridoma cell line that secretes monoclonal antibodies against BHT and its analogues, belonging to the field of immunoassay technology. The monoclonal antibodies secreted by the hybridoma cell line of this invention exhibit good sensitivity to BHT and its analogues. The monoclonal antibodies of this invention can simultaneously detect BHT, BHA, and TBHQ, and have an IC50 value for BHT. 50 The concentration was 83.08 ng / mL, and the IC50 for BHA was... 50 The concentration was 92.2 ng / mL, and the IC50 for TBHQ was [missing value]. 50 With a concentration of 86.0 ng / mL, the monoclonal antibody of this invention can simultaneously detect trace amounts of BHT, BHA, and TBHQ, and can be used for the residual detection of BHT, BHA, and TBHQ.

The application discloses K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies, hybridoma cell strains thereof and application of the K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies, sequence information of the K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies is shown as SEQ NO:1-SEQ NO:56, preservation numbers of the hybridoma cell strains are CCTCC:C2023365, CCTCC:C2023366, CCTCC:C2023367, CCTCC:C2024340, CCTCC:C2024341, CCTCC:C2024342 and CCTCC:C2024343 respectively, the K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies provided by the application can be specifically combined with capsular polysaccharide of Klebsiella, are favorable for typing of Klebsiella pneumoniae in clinic, and can prove excellent effects of the K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies in preventing and treating infection of Klebsiella pneumoniae in in-vivo and in-vitro tests, and the application provides a way for solving problems of Klebsiella pneumoniae infection and multiple drug resistance, has remarkable significance for preventing, diagnosing and treating Klebsiella pneumoniae infection, and has important value for developing a new generation of Klebsiella pneumoniae drugs.

The application belongs to the field of animal bacteriology and molecular biology technology, and relates to preparation of a hybridoma cell and application thereof. A monoclonal antibody is prepared through intracellular Lawsonia intracellularis outer membrane protein OmpA expressed by prokaryotes, and IFA, WB and blocking ELISA and other immunological detection methods for detecting or diagnosing Lawsonia intracellularis are established through the expressed intracellular Lawsonia intracellularis outer membrane protein OmpA and the prepared monoclonal antibody. The blocking ELISA for detecting serum antibodies of Lawsonia intracellularis can detect serum samples of suspected Lawsonia intracellularis infection from different species, and does not exist cross reaction with positive serum of common pig pathogens, has high sensitivity and specificity, is good in repeatability, and is suitable for high-throughput detection of serum samples.

This invention discloses a hybridoma cell line that secretes a canine OSMRβ monoclonal antibody and its applications. The monoclonal antibody binds to the extracellular terminal region of canine OSMRβ cells, exhibiting high affinity and specificity, and can be used for immunofluorescence detection, flow cytometry, and ELISA. The monoclonal antibody of this invention shows significant application potential in the development of canine OSMRβ detection reagents and diagnostic reagents for diseases and symptoms related to canine OSMRβ.

The application provides a hybridoma cell strain secreting a monoclonal antibody against a alginate mannuronic acid tetrasaccharide epitope, a monoclonal antibody and application. In order to enhance the immunogenicity of the mannuronic acid tetrasaccharide, a glycoconjugate KLH-1 is used as an immunogen to inject and immunize a mouse, so that the mouse is stimulated to produce a specific immune response against the mannuronic acid tetrasaccharide epitope, then spleen cells of the immunized mouse are fused with myeloma cells, and after screening, an initial hybridoma cell strain is obtained; then after subcloning and specific screening, a hybridoma cell strain capable of stably secreting a monoclonal antibody of the target mannuronic acid tetrasaccharide epitope in alginate is obtained, and a monoclonal ascites antibody is further obtained. The monoclonal ascites antibody shows specific recognition and combination ability for pseudomonas aeruginosa, and the combination activity is related to the expression level of alginate on the surface of the bacteria. Therefore, the monoclonal ascites antibody can be used as a precise detection and diagnosis tool for pseudomonas aeruginosa, and can also be applied to the antibacterial treatment of pseudomonas aeruginosa infection.

This invention relates to a hybridoma cell line, its secreted mouse anti-human Hb monoclonal antibody, and its applications, belonging to the fields of immunology and in vitro diagnostics. A hybridoma cell line is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: C2023339. The hybridoma cell line prepared by this invention can stably secrete mouse anti-human Hb monoclonal antibodies. The mouse anti-human Hb monoclonal antibodies secreted by the hybridoma cell line have characteristics such as small batch-to-batch variability and high affinity. The detection kit prepared using the mouse anti-human Hb monoclonal antibody secreted by the hybridoma cell line of this invention has a wide linear range, high specificity, and good positive / negative differentiation.

The present application relates to a hybridoma cell strain secreting a dimethomorph monoclonal antibody and its application, and belongs to the field of food safety immune detection. The hybridoma cell strain DBL secreting a dimethomorph monoclonal antibody has been preserved in the China General Microbiological Culture Collection Center on April 17, 2025, with a preservation number of CGMCC No. 46510, a preservation address of No. 3, Beichen West Road, Chaoyang District, Beijing, and a classification designation of a monoclonal cell strain. The dimethomorph monoclonal antibody obtained by using the strain to secrete can be used for the analysis and detection of dimethomorph residues in food safety detection. The dimethomorph monoclonal antibody cell strain obtained by the present application can be used for immune analysis and detection, and has good detection sensitivity (IC 50 value of 104.49 ng / mL) for dimethomorph.

The invention discloses a salmonella H: i epitope specific monoclonal antibody and application thereof, the salmonella H: i epitope specific monoclonal antibody is secreted by hybridoma cells with the preservation number of CCTCC NO: C202645, and the antibody has the advantages of high titer and strong specificity. According to the blocking ELISA detection kit constructed by the antibody and the detection method of the blocking ELISA detection kit, the antibody containing H: i antigen epitope salmonella in infected animal serum can be specifically detected, the antibody does not react with antibodies containing no H: i antigen epitope salmonella and non-salmonella, the sensitivity, specificity and stability are good, and the detection method is simple and convenient. And a new method is provided for direct serological detection of H: i antigen epitope-containing salmonella infection of mouse typhimurium, kafenkii and the like.

This invention provides a monoclonal antibody against the VP2 protein of a mandarin fish two-segmented RNA virus, its preparation method, and its application. The VP2 protein was obtained by expression and purification using recombinant plasmids. Mice were immunized three times, and mice with the highest serum titer were selected for intraperitoneal booster immunization against VP2 protein. Mouse spleen cells and SP2 / 0 myeloma cells were fused, and hybridoma cell lines secreting VP2 protein antibodies were screened. These cells were then subcloned to obtain four stable hybridoma cell lines secreting anti-VP2 protein antibodies, and their heavy and light chain variable region sequences were determined. Mice were injected intraperitoneally with Freund's incomplete adjuvant, followed by the injection of the hybridoma cell lines. Ascites fluid was collected from the mice, extracted, purified, and used to obtain the monoclonal antibody against the VP2 protein. Using these four monoclonal antibodies, three highly conserved and specific antigenic epitopes of the VP2 protein of the mandarin fish two-segmented RNA virus were screened and identified. This invention provides support for establishing specific serological methods for mandarin fish two-segmented RNA viruses and for further research on the biological function and clinical diagnosis of VP2.

The present application relates to a kind of bifenthrin secreting monoclonal antibody hybridoma cell strain and its application, belong to immune detection technical field.The present application is prepared by screening and obtains a kind of bifenthrin secreting monoclonal antibody hybridoma cell strain, the monoclonal antibody of hybridoma cell strain of the present application has good sensitivity and specificity to bifenthrin, specifically, the IC 50 Value of the monoclonal antibody of the present application to bifenthrin is 1.84 ng / mL, and the cross reactivity of bifenthrin analog (imidacloprid, buprofezin, pymetrozine, chlorbenzuron, diflubenzuron, metalaxyl) is less than 10%, so low concentration bifenthrin can be accurately detected.

This invention discloses a monoclonal antibody against porcine pseudorabies virus gE protein, a hybridoma cell line, and their applications, belonging to the field of biotechnology. The monoclonal antibody 3G10 comprises a light chain and a heavy chain; the amino acid sequence of the variable region of the light chain is shown in SEQ ID NO.1, and the amino acid sequence of the variable region of the heavy chain is shown in SEQ ID NO.8. This invention obtains a hybridoma cell line 3G10 that secretes a specific monoclonal antibody against porcine pseudorabies virus gE protein, and establishes a gE antibody blocking ELISA method and a gE antibody blocking ELISA kit based on the monoclonal antibody 3G10 secreted by this cell line. This method exhibits high sensitivity and specificity for detecting porcine pseudorabies virus gE antibodies, providing a new approach for the monitoring and control of porcine pseudorabies.

The application relates to the technical field of immunoglobulin, in particular to an anti-human RB protein monoclonal antibody and application thereof, a hybridoma cell strain and a kit. The monoclonal antibody is secreted by a hybridoma cell strain OTI12B5, and the hybridoma cell strain OTI12B5 is preserved in the China General Microbiological Culture Collection Center on April 26, 2025, and the preservation number is CGMCC No.46357. The hybridoma cell strain OTI12B5 provided in the application can stably secrete the anti-human RB protein monoclonal antibody, and the anti-human RB protein monoclonal antibody can be specifically combined with the RB protein, significantly improves the specificity, accuracy and reliability of RB protein immunodetection, can be used for labeling of the RB protein, and therefore can detect the RB protein content in tissues and dynamically evaluate the change of the RB protein in targeted treatment.

The present application relates to a hybridoma cell strain, monoclonal antibody, test strip, kit and application thereof for feline leukemia virus, and belongs to the field of immunological technology. The present application uses feline leukemia virus P27 protein to immunize mice, and obtains specific hybridoma cell strains FeLV-06D8F10 strain and FeLV-02B6H12 strain from mouse ascites, purifies monoclonal antibodies, and applies them in detecting feline leukemia virus. The test strip described in the present application adopts the principle of colloidal gold immunochromatography, the kit detection method described in the present application is simple and easy to operate, can simultaneously detect feline leukemia virus antigens in different media of saliva and blood of cats, and realizes detection of feline leukemia virus antigens in different media. The saliva of pet cats in families is easier to collect, realizes self-detection of pet cats in families, and has a broad application prospect.

This invention provides a neutralizing monoclonal antibody 3E3 against the hemagglutinin protein of H7 subtype influenza virus and its applications. This antibody specifically recognizes the hemagglutinin protein of H7 subtype influenza virus and exerts an antiviral effect through neutralization. The hybridoma cells that produce this monoclonal antibody are obtained by fusing BALB / C mouse spleen lymphocytes immunized with purified hemagglutinin protein and mouse myeloma cells SP2 / 0 through a process of cloning, selection, and passage, ultimately resulting in cells that stably secrete antibody 3E3. The amino acid sequence of the heavy chain variable region of the antibody is shown in SEQ ID No. 2, and the amino acid sequence of the light chain variable region is shown in SEQ ID No. 4. 3E3 can effectively bind to, neutralize, and treat H7 subtype influenza virus infection.

The application discloses a monoclonal antibody and application. A monoclonal antibody is secreted by a hybridoma cell strain 6B12G10, and an antigen epitope recognized by the monoclonal antibody is aa130-aa134 of a P30 protein of a gene I type African swine fever virus. An amino acid aa131 of the P30 protein is E. The application prepares a monoclonal antibody capable of identifying the P30 antigen of the gene I type African swine fever virus; the monoclonal antibody can directly distinguish the gene I type and the gene II type, the gene I / II recombinant type ASFV by using an indirect immunofluorescence technology; for type identification after infection of an ASFV weak strain, the application establishes a blocking ELISA method, and for the first time, effectively distinguishes whether the gene I type ASFV is infected or the gene II type, the gene I / II recombinant type ASFV is infected, effectively fills a differential diagnosis blank in the domestic and foreign aspects, and has important significance for tracing a gene I type ASFV infection.

This invention relates to a hybridoma cell line that secretes linezolid monoclonal antibodies and its applications, belonging to the field of immunoassay technology. Through multiple screening processes, this invention has obtained a hybridoma cell line that secretes linezolid monoclonal antibodies. The linezolid monoclonal antibody secreted by this hybridoma cell line exhibits excellent affinity and sensitivity to linezolid, with a high IC50 value for linezolid. 50 The concentration reaches 50 ng / mL, and there is no cross-reactivity with linezolid structural analogs, such as terdizolid and contezolid. Therefore, the monoclonal antibody of this invention can be used to prepare immunoassay products for linezolid, providing an efficient detection method and means for the detection of linezolid residues in food.

The present application relates to the technical field of immunology and meat product adulteration meat source identification, and provides a hybridoma cell strain, an anti-fox skeletal muscle troponin monoclonal antibody produced by the hybridoma cell strain and application of the antibody, the preservation name of the hybridoma cell strain is: the hybridoma cell strain skTn-3A4 of the anti-fox skeletal muscle troponin monoclonal antibody, the preservation place is China Center for Type Culture Collection, the preservation date is October 10, 2023, and the preservation number is CCTCC NO: C2023302.The anti-fox skeletal muscle troponin monoclonal antibody skTn-3A4 provided by the present application can be used for preparing an enzyme-linked immunoassay kit, a colloidal gold chromatography test strip and an evanescent wave fluorescence immune sensor for detecting fox skeletal muscle troponin, so that the fox skeletal muscle troponin in food can be rapidly and sensitively detected.

This invention discloses a high-affinity mouse anti-eel IgM monoclonal antibody, a hybridoma cell line, and its applications. The mouse anti-eel IgM monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The CDR region of the heavy chain variable region contains three sequences as shown in SEQ ID NO. 1-3, and the CDR region of the light chain variable region contains three sequences as shown in SEQ ID NO. 4-6. The mouse anti-eel IgM monoclonal antibody can be secreted by the hybridoma cell line ArIgMH-33G9D2G7C12, with accession number CCTCC NO: C202545. The mouse anti-eel IgM monoclonal antibody provided by this invention can be used for flow cytometry to identify eel IgM. + This monoclonal antibody, developed for B cells, can be used for the specific detection of secretory IgM in eels via ELISA and Western blot. It exhibits high specificity, sensitivity, and affinity, making it suitable for structural analysis of eel IgM and detection of immune response levels. This lays the foundation for in-depth research on the eel immune system and the establishment of methods for evaluating vaccine efficacy using antibody levels as an indicator.

The present application relates to a hybridoma cell strain secreting anti-reserpine monoclonal antibody and its application, and belongs to the technical field of drug safety immune detection. The hybridoma cell strain secreting anti-monoclonal antibody has been preserved in the China General Microbiological Culture Collection Center on April 17, 2025, with a preservation number of CGMCC No. 46508, a preservation address of No. 3, Xibaixili, Chaoyang District, Beijing, and a classification designation of monoclonal cell strain. The reserpine monoclonal antibody cell strain obtained by the present application can be used for reserpine analysis and detection in drugs and food, has good detection sensitivity (IC 50 value of 0.579 ng / mL) and specificity (cross rate of less than 1% for related structural analogues), can realize the detection of reserpine in drugs, can assist clinical diagnosis, and has practical application value.

This invention provides a hybridoma cell line, an anti-H. pylori monoclonal antibody, and their applications. The hybridoma cell line, named OTI2C8, is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 45713. The anti-H. pylori monoclonal antibody is secreted by the hybridoma cell line OTI2C8. The hybridoma cell line OTI2C8 described in this invention can stably secrete an anti-H. pylori monoclonal antibody that specifically binds to Helicobacter pylori (H. pylori), significantly improving the specificity, sensitivity, accuracy, and reliability of H. pylori immunodetection. It is applicable to the detection of H. pylori infection in various tissues.